276 results about "Gibberellin" patented technology

The invention belongs to the technical field of biological agents, and particularly relates to ginsenoside variant flavobacterium DG-16 and application thereof in relieving continuous cropping obstacles of angelica sinensis and improving the quality of the angelica sinensis. According to the ginsenoside variant flavobacterium DG-16 disclosed by the invention, the growth of angelica sinensis is promoted by increasing the contents of jasmonic acid and cytokinin hormones, reducing the contents of gibberellin and salicylic acid hormones and promoting the circulation and utilization of nitrogen; in addition, the flavobacterium ginsenoside variant DG-16 can significantly increase the chlorophyll content, is beneficial to photosynthesis of the angelica sinensis, effectively changes the physiological and biochemical states of the angelica sinensis, can resist root rot of the angelica sinensis so as to relieve successive cropping obstacles and improve the quality, and has a significant effect.

The invention discloses a preparation technology of a special cistanche inoculation block with a seed dormancy breaking self-induction function by combining a cistanche seed dormancy breaking inducer and hydrogel, and relates to the technical field of cistanche cultivation. The specific technical process is as follows: preparation of a dormancy breaking inducer solution, preparation of a hydrogel block containing the dormancy breaking inducer and cistanche seeds, and preparation of an inoculation block. According to the method, gibberellin and cytokinin are used as dormancy breaking inducers, and a yeast aqueous extract is used as an auxiliary agent. The problems of seed dormancy breaking, germination promoting and water retention inoculation are effectively solved, seed dormancy breaking does not need to be conducted in advance before inoculation, meanwhile, hydrogel has high water absorption and water retention capacity, the problem that dormancy breaking seeds are inactivated due to the fact that the dormancy breaking seeds cannot germinate and parasitize in time after being inoculated due to water shortage is effectively solved, and the survival rate of the seeds is increased. The inoculation efficiency is improved to 75% or above from about 20% of existing inoculation paper inoculation, and the method has a good popularization prospect and has very important significance in promoting the industrial development of cistanche deserticola.

The invention provides a grifola frondosa liquid culture medium as well as a culture method and application thereof, and relates to the technical field of fermentation. The invention provides a grifola frondosa liquid culture medium, which contains glucose, peptone, KH2P2O4, MgSO4, vitamin B1, gibberellin and tyrosol, can significantly improve the growth of grifola frondosa thalli and the secretion of exopolysaccharides, and can be used for the preparation of food, drugs and health care products.

The present invention discloses the use of a gibberellin-deficient mutant in promoting spore production in arbuscular mycorrhizal fungi. The present invention utilizes the hairy roots of the gibberellin-deficient mutant as a host plant to inoculate the arbuscular mycorrhizal fungi, significantly increasing the spore production of the arbuscular mycorrhizal fungi. The hairy roots of the gibberellin-deficient mutant can significantly increase the spore production of the arbuscular mycorrhizal fungi under both normal pH conditions and acidic conditions. The gibberellin-deficient mutant can be widely used to promote spore production in arbuscular mycorrhizal fungi. The present invention also provides a preparation and method for promoting spore production in arbuscular mycorrhizal fungi. The preparation and method can propagate the arbuscular mycorrhizal fungi under sterile conditions with high propagation efficiency, significantly increase the spore production of the arbuscular mycorrhizal fungi, are simple to operate, low in cost, and have a short cycle time, thus having broad prospects for promotion and application.

The invention relates to the technical field of rice seed germination accelerating systems and methods. Seed selection is conducted, specifically, rice seeds which are full and free of damage are screened, and impurities are removed; the rice seeds are soaked in a plant growth regulator mixed solution to be subjected to oscillation treatment, the mixed solution is composed of gibberellin with the concentration being 20-50 mg / L, pimacol with the concentration being 5-15 mg / L and polyoxyethylene lauryl ether with the concentration being 0.01-0.03%, the liquid temperature is kept at 30 + / -2 DEG C, and the treatment time is 2-4 hours; gradient disinfection: primary disinfection: putting the pretreated seeds into a 0.3-0.5% hydrogen peroxide solution at the temperature of 40-50 DEG C, and stirring for 15-20 minutes; secondary disinfection: transferring to a 0.8-1.2% sodium hypochlorite solution at the temperature of 25-30 DEG C, adding a 0.05-0.1 mol / L phosphate buffer solution to adjust the pH value to 6.8-7.2, and standing for 40-60 minutes; the disinfected seeds are put into magnetized water containing 0.1%-0.3% of chitosan derivatives and 0.05%-0.15% of sodium alginate, the magnetic field intensity ranges from 300 gauss to 500 gauss, and ultrasonic waves of 30 kHz to 50 kHz are adopted for assisting permeation for 1 h to 2 h; the rice seed germination accelerating system and method are high in germination accelerating efficiency.

The invention belongs to the technical field of plant seedling raising, and relates to a method for breaking dormancy of iris ensata thunb seeds and accelerating germination, which comprises the following steps: (1) soaking disinfected iris ensata thunb seeds in hydrogen peroxide with the concentration of 3-7wt% for 15-30min; and (2) at 25-30 DEG C, soaking with a mixed solution of gibberellin 3 and indoleacetic acid, and accelerating germination. Hard seed coats are broken through H2O2 soaking treatment, seed coat obstacles and physiological dormancy of the iris ensata thunb seeds are broken through combination of a GA3 + IAA seed soaking mode and fine sand covering germination acceleration, hormones needed by seed germination are supplemented, germination inhibitors existing in the seeds are inhibited, dormancy of the iris ensata thunb seeds is comprehensively relieved, the germination rate of the iris ensata thunb seeds is remarkably increased, and the germination rate of the iris ensata thunb seeds is increased. The germination rate is increased to 80% or above, the germination time can be further shortened, and technical support is provided for large-scale production and application of iris ensata thunb.

The invention relates to the technical field of flower flowering phase regulation and control, and discloses a regulator and a method for promoting secondary flowering of crape myrtle, the regulator comprises 50 parts by weight of benzylaminopurine, 4.6-5 parts by weight of compound sodium nitrophenolate and more than or equal to 100 parts by weight of an amino acid composition; or 18 parts by weight of benzylaminopurine, 18 parts by weight of gibberellin, 4.6-5 parts by weight of compound sodium nitrophenolate and more than or equal to 100 parts by weight of an amino acid composition. The regulator provided by the invention comprehensively acts on multiple aspects of cell division, dormancy breaking, hormone regulation, nutrition supply, stress resistance enhancement and the like to jointly promote secondary blooming of the crape myrtle, increase the blooming amount and prolong the blooming period, and by virtue of multiple aspects of effects of pruning, fertilization, insect killing, plant growth regulator regulation and the like, the yield of the crape myrtle is increased. Secondary blooming of the crape myrtle is promoted, the blooming rate, the blooming duration and the blooming amount of secondary blooming are increased, and the growth vigor of the crape myrtle in the next year is not affected.

The present invention relates to the field of allergens and more specifically to a novel isolated allergen from the species Cupressaceae shown to be a primary sensitizer for severe peach allergy mediated by the protein Pru p7. The allergen is homologous with Pru P7 in peach, a protein belonging to the Gibberelin Regulating Proteins (GRPs). The novel allergen also 5 shares high sequence homology among other species from the Cupressaceae family and there is therefore also provided additional novel allergens in two other species of the Cupressaceae family. The novel allergens find use in the in vitro diagnosis of, treatment and / or prevention of Type 1 Cupressaceace pollen allergy and Cupressaceace pollen-associated food allergies.

The invention discloses a method for adjusting the pore opening degree of Shanghai green, and belongs to the technical field of plant detection. Shanghai leaves are placed in 20mL of open liquid containing (1) 0.5% potassium chloride and 100 [mu] M / L calcium chloride or (2) 0.5% potassium chloride and 50 [mu] M / L brassinolide or (3) 0.5% potassium chloride and 10 [mu] M / L cytokinin or (4) 0.5% potassium chloride and 5 [mu] M / L gibberellin or (5) 0.5% potassium chloride and 100 [mu] M / L methyl jasmonate, culture is performed under illumination, and the illumination time is set to be 5h, so that the pore opening degree of Shanghai is remarkably increased. Compared with a control group (containing 0.5% of potassium chloride), the pore openness of the treatment is respectively increased by 33.5%, 43.5%, 43.4%, 27.1% and 70.2%. The method disclosed by the invention lays a technical foundation for rapidly screening out the exogenous environmental stimulation factor (environical stimuli) capable of increasing the opening degree of the pore.

The invention discloses a nutrition regulating agent for regulating the mature period of citrus fruits and application of the nutrition regulating agent. The nutrition regulating agent is prepared from potassium nitrate, monopotassium phosphate, magnesium sulfate, ferric sodium ethylenediaminetetraacetate, zinc sodium ethylenediaminetetraacetate, boric acid, ammonium molybdate, gibberellin, abscisic acid and diatomite which is supplemented to 100 parts. The invention further provides a preparation method of the nutrition regulating agent and application of the nutrition regulating agent in regulating the mature period of citrus fruits, and the application mode is that when the diameter of the citrus fruits is 40-50 mm, the preparation is diluted by 600-800 times to be sprayed on leaf surfaces, once every 7 days and twice continuously. Through the synergistic effect of mineral nutrient elements and low-concentration hormones, bidirectional precise regulation and control of the citrus mature period are achieved, fruits can be promoted to mature in advance or later for 7-15 days, the sugar-acid ratio and color of the fruits can be remarkably improved, meanwhile, the problems of tree vigor decline and quality decline caused by single hormone treatment are solved, and the method is suitable for large-scale popularization and application. The method has the characteristics of high efficiency, safety and simple operation.

The invention discloses a seedling raising and transplanting method for mangrove plant Aegiceras corniculatum based on external application of gibberellin, and belongs to the technical field of saline-alkali plant cultivation and mud flat ecological restoration. The method mainly comprises the four stages of seed disinfection and gibberellin soaking, germination cultivation, seedling cultivation, salinity domestication and mud flat transplanting. After being disinfected, the seeds are soaked in a gibberellin solution with the concentration being 300 mg / L, gibberellin with the same concentration is sprayed regularly during germination, and the germination rate and the germination speed are increased. In the seedling stage, humus soil and mud flat bottom mud mixed matrix is adopted for cultivation, and growth is promoted by spraying 100 mg / L gibberellin to leaf surfaces and injecting 25 mg / L gibberellin to rhizosphere. After seedlings are formed, the seedlings are subjected to step-by-step salinity domestication (5 per thousand to 30 per thousand), gibberellin spraying and rhizosphere injection are carried out at regular intervals, and the stress resistance of the seedlings is enhanced. The method can effectively shorten the seedling raising period of Aegiceras corniculatum, improve the seedling raising efficiency, enhance the adaptive capacity of seedlings to the tidal zone environment, and facilitate rapid recovery of a mangrove forest ecosystem.

The invention provides an under-forest ecological allium victorialis planting method and application, and the method comprises the following steps: after gibberellin treatment and low-temperature sand storage germination acceleration of seeds, sowing and seedling raising are performed in an under-forest or sunshelter high bed with the canopy density of 0.3-0.5, and 2-year-old seedlings are cultivated; transplanting in early April or late September according to the line spacing of 15 cm and the plant spacing of 4-6 cm, and cultivating to obtain 3-4-year-old strong seedlings; then a forest stand with the canopy density of 0.5-0.7 is selected, and field planting is conducted according to the line spacing of 20-30 cm and the plant spacing of 15-30 cm; through intertillage weeding, fertilization and pest and disease management, seedlings are harvested by adopting a three-year ring harvesting method or tender leaves are harvested by adopting a leaf breaking method. According to the method, the problems of low emergence rate, unstable yield, uneven quality and the like caused by direct planting in a wild environment are effectively solved, high-quality, stable-yield and efficient production of allium victorialis is realized, and a reliable mode is provided for utilization of northern forests and development of under-forest economy.

The invention provides a daphne giraldii seed composite treatment solution with epimedium polysaccharide as a main effective component and application, and belongs to the technical field of plant seed treatment, the daphne giraldii seed composite treatment solution comprises 100-300 mg / L of gibberellin, 0.1-100 mg / L of epimedium polysaccharide, 15-35 mg / L of 6-benzylamino adenine, 50-150 mg / L of oligosaccharide, 1-30 mg / L of benzothiadiazole, 20-60 mg / L of a bactericide and 0-1 mL / L of a surfactant. According to the application, the daphne giraldii seeds are treated with the daphne giraldii seed composite treating fluid. According to the composite treatment liquid and the treatment method, the germination rate and the germination potential of the seeds can be remarkably improved, mildewing in the stratification period is effectively inhibited, the growth potential of seedlings is enhanced, a standardized and repeatable operation process is provided, and the composite treatment liquid and the treatment method are suitable for large-scale production.

The present application is suitable for the field of molecular biology and biotechnology, and provides a maize gibberellin 13-hydroxylase gene ZmGA13ox1 and application thereof. The maize gibberellin 13-hydroxylase gene ZmGA13ox1 has a nucleotide sequence shown as SEQ ID NO:1 in the sequence listing. The maize gibberellin 13-hydroxylase protein is encoded by the maize gibberellin 13-hydroxylase gene ZmGA13ox1, and has an amino acid sequence shown as SEQ ID NO:2 in the sequence listing. The present application provides the nucleotide sequence of the ZmGA13ox1 gene in the maize inbred line B73 and the amino acid sequence information of the protein encoded thereby, and uses the agrobacterium-mediated transformation technology to introduce a plant expression vector containing the gene into Arabidopsis thaliana, and cultivates a homozygous T3 generation transgenic Arabidopsis thaliana plant. The experimental results show that the overexpression of the ZmGA13ox1 gene in Arabidopsis thaliana has a promoting effect on the root growth.

The invention discloses a germination accelerating method for pendant flower seeds with different flower colors. According to the method disclosed by the invention, the fact that different flower colors of pendant flowers have different sensitivities to different germination accelerating modes is revealed and found for the first time, white pendant flower seeds and red pendant flower seeds are subjected to germination accelerating in a clear water seed soaking manner, so that a relatively good germination effect can be obtained, and pink pendant flower seeds are subjected to germination accelerating in a gibberellin aqueous solution seed soaking manner, so that a relatively good germination effect can be obtained; a good germination effect can be obtained by accelerating germination of the red and white pennycress seeds by soaking the seeds after skin damage, and a good germination effect can be obtained by accelerating germination of the red powder pennycress seeds by soaking the seeds in clear water and then depositing and accelerating germination. Technical support is provided for formulating a personalized scheme in the process of artificially breeding the pendant flowers and improving the seed germination rate and the seedling quality.

The present invention is directed to mixtures comprising 6-benzyladenine, gibberellic acid 4 / 7 and a third compound selected from the group consisting of proline and malic acid. The present invention is further directed to methods of increasing plant, growth, yield and / or quality comprising applying sequentially or concurrently an effective amount of mixtures of the present invention to the plant or an area where the plant will grow.

The application of a soybean GmGASA1 gene, the application relates to the application of a gibberellin regulation related protein GmGASA1 coding gene of soybean.The application of the soybean GmGASA1 gene, namely the application of improving the protein content, the seed amino acid content and the grain weight of plants; the application of the soybean GmGASA1 gene in plant breeding; the application of the soybean GmGASA1 gene in cultivating transgenic plants.The experimental results show that three transgenic lines are obtained by overexpressing the GmGASA1 gene in soybean, the protein content and the grain weight of the transformed soybean seeds are significantly improved compared with the seeds of the untransformed receptor plants, which indicates that the GmGASA1 gene can regulate the protein content, the seed amino acid content and the grain weight of seeds.The GmGASA1 gene can be used for improving the quality of soybean and increasing the yield of soybean.The application of the GmGASA1 gene can effectively realize the breeding of new soybean varieties and the creation of new germplasm.

The invention relates to a method for out-of-season regulation and control of blooming of curcuma alismatifolia. Comprising the following steps: after planting curcuma alismatifolia, applying a gibberellin antagonist or a gibberellin inhibitor in a vegetative growth period; light supplementation is started before the illumination in the greenhouse is lower than a light compensation point, light supplementation is conducted for 3.5-4.5 hours every day, and the light supplementation intensity is 80-100 mu mol / m < 2 > s; after field planting, the temperature of the greenhouse in the daytime is not lower than 20 DEG C, and the temperature at night is not lower than 15 DEG C. By controlling the temperature and prolonging the illumination, the method for regulating and controlling the blooming of the curcuma alismatifolia in an out-of-season mode is established, out-of-season cultivation of the curcuma alismatifolia is achieved, the curcuma alismatifolia can bloom before the spring festival and appear on the market during the spring festival, the requirements of people for flower appreciation and flower using in festivals are met, and the flower market in the festivals is enriched.

The application discloses a new method for synthesizing dihydrogibberellin without protecting carboxyl. The application provides a new method for synthesizing dihydrogibberellin without protecting carboxyl, which comprises the following steps: 1) mixing a compound shown in formula II with a catalyst A in an organic solvent B, and carrying out a hydrogen reduction reaction under a hydrogen atmosphere to obtain a compound shown in formula III; 2) mixing the compound shown in formula III with a saturated aqueous solution of a base in an organic solvent C, and carrying out a selective deacetylation reaction to obtain a compound shown in formula IV; and 3) mixing the compound shown in formula IV with triphenyl phosphine and an elimination reagent in an organic solvent D, and carrying out a dehydration elimination reaction to obtain dihydrogibberellin shown in formula V. The application can simplify the synthesis method of dihydrogibberellin and is more beneficial to large-scale production and synthesis.

The invention provides a composite initiator for improving the germination rate of teasel seeds and application of the composite initiator, and belongs to the technical field of plant seeds. The composite initiator disclosed by the invention is prepared from gibberellin GA3, polyethylene glycol 6000 and CaCl2. Experiments show that various germination indexes of the single initiator PEG6000 and CaCl2 are superior to those of GA3, different proportions of the composite initiator have obvious influence on the germination indexes, the germination rate of seeds treated by the composite initiator provided by the invention is the highest, and the initiation effect on the length of the overground part of teasel and the weight of a single plant is superior to that of the single initiator. According to the method, the germination rate of the teasel seeds can be increased, meanwhile, the growth of teasel seedlings can be effectively promoted, and the seedling strengthening effect is achieved.

The invention relates to the technical field of gene engineering, in particular to a ZmPIF4 gene with heat resistance and application of the ZmPIF4 gene, the nucleotide sequence of the ZmPIF4 gene is shown as SEQ ID NO.1, the ZmPIF4 gene is mainly located in a cell nucleus and possibly plays an important role in the cell nucleus signal transduction process, and under high temperature stress, the ZmPIF4 gene can serve as a heat-resistant gene. The corn ZmPIF4 gene can respond to high-temperature stress through up-down regulation expression, the corn ZmPIF4 gene can increase the GA content, JA content and IAA content of corn plants, gibberellin has the most prominent effect of accelerating cell elongation and promoting cell expansion, and therefore the high-temperature stress resistance of the corn plants can be improved.

The invention discloses a dandelion seed coating agent and a preparation method and application thereof.The seed coating agent comprises a growth regulator, a bactericide and an insecticide, the growth regulator comprises 2, 4-epibrassinolide, monopotassium phosphate and gibberellin, the bactericide comprises difenoconazole, metalaxyl-M and fludioxonil, the insecticide comprises abamectin and thiamethoxam, and the insecticide comprises a thiamethoxam aqueous solution. The seed coating agent is prepared from the following effective components in percentage by mass: 1 to 2 percent of 2, 4-epibrassinolide, 1 to 2 percent of monopotassium phosphate, 0 to 0.1 percent of gibberellin, 0.1 to 0.3 percent of difenoconazole, 0.1 to 0.2 percent of metalaxyl-M, 0.1 to 0.2 percent of fludioxonil, 0.1 to 0.3 percent of thiamethoxam, 0.1 to 0.3 percent of abamectin and the balance of water. After seeds are treated by the seed coating, pathogenic bacteria carried by the dandelion seeds can be killed, the activity and disease resistance of the dandelion seeds are remarkably improved, and the vegetative growth yield of dandelions is increased.

The invention relates to the technical field of grape seed germination methods, and discloses a method for promoting rapid germination of grape seeds, which comprises the following steps: collecting ripe grape fruits, stripping the seeds, washing with clear water for 3-5 times, drying the seeds in the shade, sub-packaging the seeds in centrifugal tubes, preserving at 4 DEG C for later use, placing the seeds in 75% alcohol, oscillating for 5 minutes, washing with sterile water for 3-5 times, and taking out the seeds. Transferring into a 1% sodium hypochlorite solution, oscillating for 30 minutes at the speed of 150r / min, cleaning with sterile water for 3-5 times, cutting off beaks and cotyledon ends of the seeds by using disinfection scissors, simultaneously cutting off the beaks and the cotyledon ends, not performing treatment, immersing the treated seeds into a 150mg / L gibberellin solution, oscillating for 8 hours at the room temperature at the speed of 150r / min, cleaning with sterile water for 3-5 times, absorbing moisture with filter paper, laying the filter paper and sterile gauze in a culture dish, and transplanting the seeds into the culture dish. Adding sterile water until the seeds are wet and free of accumulated water, setting three repetitions for each treatment group, uniformly placing 50 seeds for each repetition, culturing at 25 + / -2 DEG C, alternating illumination / darkness for 12 hours, supplementing water every 3 days, and recording the germination number and the root length.

The invention provides a method for inducing butterfly orchid to bloom, which mainly researches the secondary blooming characteristic of butterfly orchid, and promotes the blooming of butterfly orchid and shortens the secondary blooming time of butterfly orchid in combination with the use of a plant growth regulator and an environment management method. According to the technical scheme, the method is mainly used for evenly spraying an exogenous growth regulator on leaf surfaces, the exogenous growth regulator is a solution of gibberellin or cytokinin, methyl jasmonate and indole-3-acetic acid, and meanwhile environment management is matched, so that the purpose of shortening the flowering time of phalaenopsis amabilis is achieved.

The invention provides a rapid dormancy breaking method for sand whip seeds and dormancy breaking sand whip seeds, and relates to the technical field of plant seed treatment.The method comprises the following steps that 1, physical initiation treatment is conducted, specifically, the disinfected sand whip seeds are placed in warm water at the temperature of 45-55 DEG C to be soaked for 10-14 h; (2) chemical initiation treatment: soaking the seeds treated in the step (1) in a gibberellin solution with the mass concentration of 0.03%-0.07% for 20-28 hours; (3) biological seed dressing treatment: carrying out seed dressing on the seeds treated in the step (2) and a trichoderma harzianum fungicide, wherein the mass of the fungicide is 0.05-0.15% of the mass of the seeds; according to the method, the dormancy mechanism of the sand penis seeds can be effectively broken, germination of the seeds is promoted, the germination rate of the sand penis seeds treated by the optimized method can reach 87.00% on the 21st day, the germination synchronism of the seeds can be higher, and the problem of irregular natural germination is solved.

This invention discloses a method for cultivating long-branch Buddha's Hand fruit by cutting branches. The method uses a special variety called "Sunshine" and cultivates high-quality long-branch Buddha's Hand fruit by controlling the density of fruiting branches, spraying a 1500-fold dilution of 3% naphthaleneacetic acid during the bud stage to induce finger-shaped splitting, spraying a gibberellin-chlorpyrifos-potassium dihydrogen phosphate compound solution during fruit development to synergistically thicken the fruit stalk, adjusting the branch direction in summer to keep the fingertips pointing upwards, and combining this with midday shading. This method results in a 26.7% increase in fruit stalk diameter, a 6.4% increase in the number of fruits with open fingers, a 100.2% increase in the number of long-branch fruits, a 20.0% reduction in the number of fruits with fruit surface burns, and an 8.8% reduction in the number of fruits that fall off after harvest. The number of fresh-cut long-branch marketable fruits increases by 100.2%, and the long-branch marketable fruit rate reaches over 70%. Overcoming the bottlenecks of traditional cultivation such as weak fruiting ability of long branches, thin and weak fruit stalks that are easy to fall off, and easy damage to the fruit surface, the cultivated long-branch Buddha's Hand fruit has the advantages of being resistant to fruit drop, having a smooth fruit surface, having a well-developed fruit shape, and having suitable branches. It can be directly used for cut flower scenarios such as vase decoration and bouquet matching, creating a new category of Buddha's Hand cut fruit and significantly enhancing its ornamental and economic value.

The invention belongs to the technical field of plant breeding, and discloses a breeding method for improving the mutagenesis efficiency of stem tips of woody plants. The CRISPR-Cas9 ribonucleoprotein complex and plant hormones 6-benzyladenine, gibberellin and jasmonic acid are co-encapsulated and then accurately delivered to stem tip meristem of woody ornamental plants such as acer truncatum, pteroceltis tatarinowii, crape myrtle and tassel, and the gene mutation obtaining rate of the stem tip meristem is effectively increased; the intelligent environment control system is used for dynamically optimizing illumination, temperature, humidity and oxidative stress, activating a plant endogenous DNA repair pathway and further enhancing homologous recombination efficiency, meanwhile, through collaborative optimization of induction hormone signals and environmental stress, the stress resistance is improved, the leaf color, the plant type and the growth vigor can be accurately regulated and controlled, and the yield of the plant is increased. According to the method, efficient, accurate and stress-resistant linked woody plant stem tip mutation breeding is realized.

The invention discloses an underground propagule composite excitant for ecological restoration and a preparation method thereof, and relates to the technical field of excitant preparation. The fertilizer is prepared from the following raw materials in parts by weight: 0.2 to 0.3 part of gibberellin, 0.2 to 0.5 part of 6-benayl aminopurine, 0.2 to 0.3 part of indolebutyric acid, 0.5 to 0.7 part of sodium nitroprusside, 0.4 to 0.5 part of hydrogen peroxide, 1 to 2 parts of jasmonic acid sustained-release microspheres, 5 to 7 parts of compound amino acid, 1 to 3 parts of compound microbial agent sustained-release microspheres, 2 to 3 parts of seaweed extract, 10 to 13 parts of humic acid, 2 to 3 parts of monopotassium phosphate, 60 to 74 parts of ethanol and 1000 parts of water. The underground propagule composite excitant provided by the invention is beneficial to promoting activation and germination of underground propagules, enhancing plant growth and stress resistance, improving soil micro-ecological functions, forming stable diversity communities and facilitating ecological restoration.

This invention belongs to the field of grape cultivation technology, specifically relating to a fruit enlargement agent for Daqing grapes, its preparation method, and its application. The fruit enlargement agent for Daqing grapes is prepared from an aqueous solution of gibberellin, thiabendazole, and brassinolide. Specifically, the concentration of gibberellin in the fruit enlargement agent is 20 mg / L~25 mg / L, the concentration of thiabendazole is 1.0 mg / L~1.25 mg / L, and the concentration of brassinolide is 0.025 μg / L~0.050 μg / L. The fruit enlargement agent of this invention, combined with fruit thinning treatment, can increase the weight of a single Daqing grape berry by at least 20% while retaining the soluble solids content of the grape.

1.The name of the design product: packaging box (85% gibberellin acid crystal powder). 2.The use of the design product: used for packaging box containing 85% gibberellin acid crystal powder. 3.The design points of the design product: the combination of patterns and colors. 4.The picture or photo best indicating the design points: front view. 5.The design for which protection is sought contains colors.