530 results about "Gibberellin" patented technology

The invention belongs to the technical field of microorganisms, and particularly relates to bacillus mobilis DG-7 and application thereof in inhibiting bolting of angelica sinensis and improving disease resistance of angelica sinensis. The invention provides a strain of bacillus mobilis DG-7, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.31089. The bacillus mobilis DG-7 and the microbial inoculum thereof provided by the invention can play roles in inhibiting early bolting of angelica sinensis and improving disease resistance by increasing the content of plant endogenous melatonin and salicylic acid, reducing the content of abscisic acid, auxin and gibberellin, reducing the content of total phenolic acid and reducing the expression of photoperiod-related genes. In addition, the bacillus mobilis DG-7 does not solubilize phosphorus, so that the promotion effect of high-concentration phosphorus on early bolting is effectively avoided; the laccase activity of the bacillus mobilis DG-7 is high, polyphenol substances can be effectively removed, and the bolting rate can be reduced.

The invention discloses a ganoderma lucidum culture solution and a culture method thereof. Comprising the following raw materials in parts: 30-40 parts of water, 10-20 parts of corn flour, 1-5 parts of ammonium nitrate, 1-5 parts of monopotassium phosphate, 1-5 parts of potassium sulfate, 1-5 parts of boric acid, 1-5 parts of manganese sulfate, 1-5 parts of zinc sulfate, 1-5 parts of copper sulfate, 1-5 parts of ferric sulfate, 1-5 parts of a short-acting growth regulator, 1-5 parts of amino acid, 1-5 parts of vitamins, 1-5 parts of a seaweed extract, 1-5 parts of honey and 1-5 parts of peaches and oranges. The short-acting growth regulator is prepared from gibberellin, auxin and cytokinin according to the ratio of (2-5): (1-8): (2-9), and the amino acid is prepared by mixing isoleucine, lysine, methionine and phenylalanine according to the ratio of (1-5): (2-8): (4-9): (5-7). The nutrient purity of the culture solution can be greatly increased, the fatality rate is reduced, cell elongation and rooting are promoted, plant metabolism and growth can be greatly promoted, and the quality of ganoderma lucidum is improved.

The invention belongs to the technical field of biological agents, and particularly relates to bacillus siamensis DG-39, a microbial agent and application of the bacillus siamensis DG-39 to inhibition of early bolting of angelica sinensis. According to the bacillus siamensis DG-39 and the microbial inoculum containing the bacillus siamensis DG-39, the physiological and biochemical states of angelica sinensis are effectively changed mainly by increasing the content of endogenous melatonin of angelica sinensis, reducing the content of abscisic acid, auxin and gibberellin and promoting circulation and utilization of nitrogen, and the photoperiod of the angelica sinensis can be effectively improved by reducing expression of related genes of the photoperiod. The effect of inhibiting early bolting of the angelica sinensis is achieved. Meanwhile, the bacillus siamensis DG-39 microbial inoculum has the characteristics of fixing nitrogen, not dissolving phosphorus and producing siderophore, and can promote the absorption and utilization of plants on nitrogen, so that the promotion effect of excessive phosphorus on bolting is avoided; meanwhile, the bacillus siamensis DG-39 laccase is high in activity, can remove phenolamine substances for promoting bolting, and is beneficial to reducing the bolting rate.

The invention relates to the technical field of microorganisms, in particular to paenibacillus mucilaginosus HX-02 and application of the paenibacillus mucilaginosus HX-02 in promoting crop growth. The preservation number of the paenibacillus mucilaginosus HX-02 is CGMCC (China General Microbiological Culture Collection Center) No. 30949. The application comprises increasing one or more of the plant height, the branch number, the weight or the number of crops. According to the invention, the paenibacillus mucilaginosus HX-02 is obtained through screening and separation, and can decompose lattice structures of soil minerals to release K, Fe, Mg, Si, Al and Mo; secreting cytokinin, gibberellin, kinetin, indoleacetic acid and acetylsalicylic acid; therefore, the invention has important application value in the field of agricultural development.

The invention belongs to the technical field of biology, and discloses a method and application for regulating and controlling the fermentation process of schizochytrium limacinum through plant hormones to improve the oil titer, and the method is characterized in that in the fermentation process of schizochytrium limacinum, one or more plant hormones are added into a culture medium to promote accumulation of oil. Plant hormones are added into a fermentation culture medium of schizochytrium limacinum, the plant hormones comprise gibberellin (GB), 6-benayl aminopurine (6-BAP), forchlorfenuron (CPPU) and brassinolide (BR), and the plant hormones promote grease accumulation by adjusting activity of enzymes related to fatty acid synthesis, so that the plant hormones can adjust metabolic pathways of the schizochytrium limacinum and promote grease accumulation. The synthesis efficiency and the yield of the grease are obviously improved.

The invention discloses burkholderia and application thereof in tobacco growth promotion, and belongs to the technical field of microorganisms. The Burkholderia sp. XCD4 is preserved in the China Center for Type Culture Collection (CCTCC), the preservation number of the Burkholderia sp. XCD4 is CCTCC NO: M 20241837, and the preservation time of the Burkholderia sp. XCD4 is August 23, 2024. The Burkholderia cepacia XCD4 is obtained through separation for the first time, the Burkholderia cepacia XCD4 has the good capacity of dissolving phosphorus and potassium, secreting IAA, producing siderophores, producing ACC deaminase and producing gibberellin, seed germination is promoted by remarkably improving the germination rate, germination potential, seedling length and root length of seeds, plant growth can be remarkably promoted, and the yield of plants is increased. Meanwhile, enrichment of phosphorus, potassium and other elements in tobacco planting plough layer soil can be reduced, and the utilization efficiency of the fertilizer is improved, so that the application prospect is good.

The invention discloses a method for simultaneously detecting residual quantity of 19 plant growth regulators in traditional Chinese medicinal materials, and belongs to the technical field of traditional Chinese medicinal material detection. The 19 plant growth regulators in the detected traditional Chinese medicinal materials are gibberellins, 4-chlorophenoxyacetic acid, 6-benzyladenine, paclobutrazol, 3-indolebutyric acid, thidiazuron, 2, 4-dihydroxyacetophenone and 2, 4-dihydroxyacetophenone respectively. The pesticide is prepared from the following raw materials: sodium 4-dichlorphenoxyacetate, forchlorfenuron, 3-indoleacetic acid, 4-fluorophenoxyacetic acid, isopentenyl adenine, imazalil, N-N '-diphenylurea, mepiquat chloride, diethyl aminoethyl hexanoate, 1-naphthylacetic acid amine, brassin, abscisic acid and 3-indolepropionic acid. According to the method, the QUECHERS traditional Chinese medicinal material sample on-machine pretreatment technology is applied, the ultra-high performance liquid chromatography-tandem mass spectrometry is established to measure the residual quantity of the 19 plant growth regulators in the medicinal materials, and the detection method is simple, clear, few in operation steps, extremely easy to operate, good in linear relation, good in sensitivity and high in accuracy.

The invention provides a treatment method for promoting germination of Cinnamomum tamala seeds, and relates to the technical field of artificial breeding of Cinnamomum tamala. The treatment method mainly comprises the following steps: carrying out ice-water ultrasonic treatment on fresh Cinnamomum camphora fruits, removing fake peels to obtain seeds, carrying out shock cooling variable-temperature pretreatment on the seeds, preparing a nutrient solution from gibberellin, tocopheryl acetate, N-acetylglucosamine, compound vitamins and compound amino acids, wetting paper by using the nutrient solution, wrapping the seeds, carrying out sowing management and the like. According to the method, the defects in the prior art are overcome, the germination rate of fresh camellia oleifera seed sowing is effectively increased, growth of early germination seedlings of camellia oleifera is promoted, and a good guiding effect is provided for artificial breeding and planting of camellia oleifera.

The invention discloses a special leaf fertilizer for regenerative fragrant rice and application of the special leaf fertilizer. The special leaf fertilizer for the regenerated fragrant rice contains regulating substances such as triacontanol, gibberellin, diethyl aminoethyl hexanoate, pimacol, paclobutrazol and amino acid, and also contains microelement fertilizers such as zinc sulfate, ferrous sulfate, copper sulfate, manganese sulfate and ammonium molybdate. The special foliar fertilizer for the regenerated fragrant rice is appropriate in component and proper in proportion, and the yield of the regenerated fragrant rice can be effectively increased and the content of fragrant substances of the regenerated fragrant rice can be increased by applying the foliar fertilizer after first season fragrant rice is harvested. The special leaf fertilizer for the regenerated fragrant rice has the effects of increasing yield and aroma, and is beneficial for improving quality and efficiency of rice industry production.

The invention provides an organic liquid suspension fertilizer as well as a preparation method and application thereof. Comprising the following components in parts by weight: 10-15 parts of chitosan mother liquor, 12-16 parts of amino acid fermentation mother liquor, 23-27 parts of gibberellin mushroom dreg fermentation liquor, 30-35 parts of vinasse fermentation liquor, 5-10 parts of potassium humate, 1-3 parts of a surfactant, 12-15 parts of a nitrogen fertilizer, 10-14 parts of a phosphate fertilizer, 26-31 parts of a potassium fertilizer, 3-8 parts of a trace element mixture and 35-40 parts of small molecule water. The organic liquid suspension fertilizer prepared by the invention can be used as a crop fertilizer, especially can improve the quality of potatoes and the yield and sugar content of beet, and reduces brown spot.

The invention belongs to the technical field of agricultural biology, and particularly relates to a GmGAI7 gene and application of the GmGAI7 gene in soybeans. A jy425 dwarfing mutant is obtained by screening and identifying an EMS (Enhanced Mass Spectrometry) mutagenesis group of JY47 (JY47), then BSA (Bovine Serum Albumin) sequencing analysis and fine positioning are carried out on a filial generation of jy425 * JN18 to find and verify that SNP variation exists at a chr18: 3281001 site of a soybean genome, so that non-synonymous mutation (gTc / gAc) of amino acid occurs, and a molecular marker is developed aiming at the site by a KASP method; meanwhile, it is found that the soybeans are insensitive to gibberellin due to mutation (T-> A) of the GmGAI7 gene, and the plant height of the soybeans is remarkably reduced. Therefore, cognition on ideal plant types of soybeans is widened, important genetic resources are provided for molecular marker-assisted breeding, and the breeding process is more accurate, efficient and controllable.

The invention belongs to the technical field of biological agents, and particularly relates to ginsenoside variant flavobacterium DG-16 and application thereof in relieving continuous cropping obstacles of angelica sinensis and improving the quality of the angelica sinensis. According to the ginsenoside variant flavobacterium DG-16 disclosed by the invention, the growth of angelica sinensis is promoted by increasing the contents of jasmonic acid and cytokinin hormones, reducing the contents of gibberellin and salicylic acid hormones and promoting the circulation and utilization of nitrogen; in addition, the flavobacterium ginsenoside variant DG-16 can significantly increase the chlorophyll content, is beneficial to photosynthesis of the angelica sinensis, effectively changes the physiological and biochemical states of the angelica sinensis, can resist root rot of the angelica sinensis so as to relieve successive cropping obstacles and improve the quality, and has a significant effect.

The invention discloses a method for seed production and seedling breeding of clematis orientalis. The method comprises five key steps of seed shell pretreatment, seed treatment, soil transferring and planting, plant transplanting and seedling culture management. According to the method, seed shells are creatively polished by abrasive paper until the seed shells are thinned but seed kernels are not damaged, and the seed germination time is shortened to 14.08 days and the germination rate is increased to 78.33% in combination with a synergistic treatment technology of soaking in a gibberellin solution. By designing a stepped seedling culture container system and matching with a peat-perlite-vermiculite matrix ratio, accurate matching between root development and container space is realized. By adopting a temperature and humidity intelligent regulation and control and staged water and fertilizer management scheme and combining with precise application of a water-soluble fertilizer and an aoGreen A5 slow-release fertilizer, the survival rate and growth vigor of seedlings are remarkably improved. The method breaks through the traditional breeding bottleneck, shortens the breeding period, improves the seed germination rate, realizes factory-like efficient production of clematis seedlings, and has remarkable economic benefits and ecological values.

The invention discloses a preparation technology of a special cistanche inoculation block with a seed dormancy breaking self-induction function by combining a cistanche seed dormancy breaking inducer and hydrogel, and relates to the technical field of cistanche cultivation. The specific technical process is as follows: preparation of a dormancy breaking inducer solution, preparation of a hydrogel block containing the dormancy breaking inducer and cistanche seeds, and preparation of an inoculation block. According to the method, gibberellin and cytokinin are used as dormancy breaking inducers, and a yeast aqueous extract is used as an auxiliary agent. The problems of seed dormancy breaking, germination promoting and water retention inoculation are effectively solved, seed dormancy breaking does not need to be conducted in advance before inoculation, meanwhile, hydrogel has high water absorption and water retention capacity, the problem that dormancy breaking seeds are inactivated due to the fact that the dormancy breaking seeds cannot germinate and parasitize in time after being inoculated due to water shortage is effectively solved, and the survival rate of the seeds is increased. The inoculation efficiency is improved to 75% or above from about 20% of existing inoculation paper inoculation, and the method has a good popularization prospect and has very important significance in promoting the industrial development of cistanche deserticola.

The invention relates to the technical field of biological prevention and treatment, and discloses a composition for preventing and treating rice sheath blight disease, which is prepared from the following raw materials in parts by weight: 80 to 90 parts of validamycin, 30 to 45 parts of traditional Chinese medicine extract paste, 10 to 20 parts of traditional Chinese medicine raw medicine, 15 to 19 parts of white carbon black, 10 to 17 parts of copper sulfate, 12 to 16 parts of chitosan, 9 to 15 parts of gibberellin, 5 to 9 parts of tebuconazole, 1 to 5 parts of compound sodium nitrophenolate, 2 to 4 parts of emulsifier and 1 to 3 parts of synergist. Gleditsia sinensis, sanguisorba officinalis, flos rhododendri mollis and sapindus mukorossi peel in the traditional Chinese medicine raw medicines are used for synergistically killing bacteria, enhancing the immunity of plants and delaying the decomposition of validamycin, and meanwhile, extracts of honeysuckle, dandelion, croton and artemisia annua in the traditional Chinese medicine extract are complementary with validamycin in the aspects of sterilization, virus resistance and plant resistance improvement; therefore, the effect of validamycin is more durable, the duration of single application is longer, and the manpower and material resource cost is saved.

The invention relates to the technical field of agricultural planting, in particular to a method for breaking dormancy of cistanche seeds and improving germination rate, which comprises the following steps: pretreating the cistanche seeds for 24 hours by using 10mg / L fluridone, and then culturing the cistanche seeds by using 25mg / L gibberellin solution. According to the method for breaking the dormancy of the cistanche deserticola seeds and improving the germination rate, the cistanche deserticola seeds are pretreated with fluridone of 10 mg / L for 24 hours, then the germination effect of the cistanche deserticola seeds cultured with a gibberellin solution of 25 mg / L is optimal, and the germination rate of the cistanche deserticola seeds is the highest and is 59.43%.

The invention provides a planting management method for prolonging the flowering phase of rich Ning camellia nitidissima, and relates to the technical field of planting management of rich Ning camellia nitidissima in the flowering phase. The management method mainly comprises the steps that fertilizer composed of manure, 15-15-15 nitrogen-phosphorus-potassium compound fertilizer, pomelo peel fermentation materials, cooked hawthorn powder and bamboo charcoal is arranged, and fertilization treatment is conducted after camellia nitidissima is shaped and pruned; then, a nutritional agent composed of gibberellin, carbenicillin, indoleacetic acid, curcumin, alpha-pinene, ethyl isothiocyanate, glyceryl trioleate, cane sugar, urea, multivitamins and organic components is arranged for drip irrigation at the beginning of May when flower buds appear and the full-bloom stage begins, and after the flower buds turn yellow, the nutritional agent is sprayed on leaf surfaces and the flower buds for multiple times. The method overcomes the defects in the prior art, effectively prolongs the flowering period of the rich Ning camellia nitidissima, further promotes the growth of flower buds of the camellia nitidissima, and comprehensively improves the ornamental value of rich Ning camellia nitidissima planting.

The invention relates to the field of ultraviolet light absorbers, and discloses a research and development method of an ultraviolet light absorber with a novel structure. Comprising the following raw materials in parts by weight: 25 to 30 parts of pyrene-triazine hybrid, 10 to 20 parts of MOF loaded benzotriazole, 15 to 20 parts of silicon dioxide coated nano zinc oxide, 10 to 15 parts of carbon quantum dot modified titanium dioxide, 8 to 12 parts of PLGA microcapsules, 7 to 13 parts of chitosan-hyaluronic acid composite film, 12 to 18 parts of Eu < 3 + > coated MOF fluorescent material, 8 to 12 parts of gibberellin-nano selenium compound and 5 to 8 parts of ionic liquid PF6. The absorption wavelength range is widened by virtue of a unique structure of a pyrene-triazine hybrid, the stability and dispersity are improved by MOF-loaded benzotriazole through MOF pore channels, the conditions of agglomeration and poor stability of nano-zinc oxide are improved by coating nano-zinc oxide with silicon dioxide, and the photoresponse range of titanium dioxide is expanded by modifying titanium dioxide with carbon quantum dots.

The invention provides application of gibberellin oxidase and a coding gene thereof in regulation and control of plant height. The gibberellin oxidase protein has an amino acid sequence as shown in SEQ ID NO: 2. The protein is over-expressed in plants, and the plant height of the plants can be increased.

The invention discloses a podophyllum hexandrum callus rapid proliferation method, and belongs to the technical field of plant tissue culture, the podophyllum hexandrum seed dormancy is broken through a sand storage method, the seeds are soaked in distilled water and a gibberellin solution and then are fully and uniformly mixed with river sand, after storage and germination acceleration, the seeds are sown in soil added with an equal amount of matrix, and podophyllum hexandrum seedlings are obtained through culture; fresh leaves of podophyllum hexandrum seedlings are taken as explants, the explants are disinfected and cut into small pieces, the small pieces are cultured on an MS culture medium added with hormone for 30 d to induce callus, the induced callus is cultured and proliferated at the optimal temperature of 15 DEG C, and the callus is inoculated to the same fresh culture medium after being cultured for 20 d to prevent browning, so that the high-quality callus is obtained. And then replacing a fresh culture medium every 20 days to realize rapid and mass proliferation of the podophyllum hexandrum callus. Through the method, a large amount of high-quality podophyllum hexandrum callus can be rapidly obtained, and sufficient materials are provided for podophyllotoxin extraction and medicinal value development.

The invention discloses application of a'microorganism-crop 'interaction signal substance in preparation of a product, the'microorganism-crop' interaction signal substance is acetoin and 2, 3-butanediol, and the product is used for regulating and controlling a soil microbial community, improving abundance of soil phosphate solubilizing bacteria, improving activity of soil alkaline phosphatase and soil respiration intensity, and improving soil quality. The fertilizer can regulate and control metabolic pathways in crops, increase the contents of cane sugar, galactose, melibiose, dehydroascorbic acid, tryptophan, gibberellin, jasmonic acid, methyl jasmonate and ferulic acid in the crops, improve the growth and stress resistance of the crops, promote the growth of the crops, increase the yield of the crops, prevent and control crop diseases and improve the yield of the crops. The soil alkaline phosphatase activity and the microbial respiration intensity are improved. The product is a liquid fungicide, and comprises a bacterial liquid, a microorganism-crop interaction signal substance, and at least one of amino acid, seaweed essence and humic acid. The liquid fungicide can promote crop growth, improve crop stress resistance and effectively inhibit pathogenic bacteria.

The invention discloses a compound agent for improving high-temperature heat damage resistance in flowering and filling stages of rice and application of the compound agent. The effective components of the compound agent comprise 28-homobrassinolide and gibberellin GA4 + 7. Under the condition of high temperature, a compound agent mixed solution containing 0.03 mg / L of 28-homobrassinolide and 1.5 mg / L of gibberellin GA4 + 7 is sprayed one week before heading of the rice and at the full heading stage respectively. The compound agent can be used for reducing the harm of high-temperature heat damage in the flowering stage and the filling stage to the rice, and is beneficial to improving the maturing rate and the thousand seed weight, so that the rice yield is remarkably increased. The compound agent can be applied to planting areas where high-temperature heat damage frequently occurs in the flowering stage and the filling stage of rice, and has wide application value.

The invention discloses a cultivation method for improving quality and aroma of flue-cured tobaccos. The cultivation method comprises the steps that 1, the flue-cured tobaccos are planted in rainy tobacco areas in the south; 2, preparing a foliar fertilizer; the leaf fertilizer is prepared from the following components in parts by mass: 1000 parts of tobacco stem leaching liquor, 100 to 500 parts of amino acid raw powder, 50 to 100 parts of potassium nitrate, 50 to 100 parts of monopotassium phosphate, 0.005 to 0.05 part of gibberellin and 100 to 250 parts of glucose; the foliar fertilizer is prepared by adding the components in proportion and fully and uniformly mixing the components. 3, before and after topping of flue-cured tobacco plants, the prepared foliar fertilizer is used for at least one time of foliar spraying operation on fresh tobacco leaves of flue-cured tobacco. According to the quality-improving and aroma-enhancing cultivation method for the flue-cured tobacco, nitrogen, phosphorus and potassium nutrient elements and plant hormones, namely gibberellin and glucose are reasonably added into the leaf fertilizer, so that sufficient growth, natural maturity and chemical component coordination regulation of the tobacco leaves are realized, and the aroma quality of the flue-cured tobacco is improved.

The invention discloses a gene ZmKO1 for regulating flowering and pollination of corn male flower spikelet and application of the gene ZmKO1 in corn male sterility hybrid seed production, and belongs to the field of plant genetic engineering. The invention provides a gene ZmKO1 for regulating and controlling flowering and pollination of maize male flower spikelet, and the phenomenon of nuclear male sterility can be caused by mutation of the gene ZmKO1. It is also clear that on the basis of the ZmKO1 gene locus, a CRISPR-Cas9 technology or a mutant gene backcross transformation technology is utilized, and cultivation of the maize nuclear male sterile line can be achieved. The invention also finds that in the spinning period of the sterile line, the male flower fertility of the sterile line can be recovered by spraying exogenous gibberellin to tassels, normal selfing propagation can be realized, and the sterile line can be economically and efficiently maintained as a maintainer line. Therefore, the cell nucleus male sterile line created based on the ZmKO1 gene locus has the characteristic of dual purposes and can be used for maize male sterility hybrid seed production, and the invention also provides a technical system for realizing maize two-line method sterile hybrid seed production by utilizing ZmKO1 gene mutation.

The invention provides a grifola frondosa liquid culture medium as well as a culture method and application thereof, and relates to the technical field of fermentation. The invention provides a grifola frondosa liquid culture medium, which contains glucose, peptone, KH2P2O4, MgSO4, vitamin B1, gibberellin and tyrosol, can significantly improve the growth of grifola frondosa thalli and the secretion of exopolysaccharides, and can be used for the preparation of food, drugs and health care products.

The present invention discloses the use of a gibberellin-deficient mutant in promoting spore production in arbuscular mycorrhizal fungi. The present invention utilizes the hairy roots of the gibberellin-deficient mutant as a host plant to inoculate the arbuscular mycorrhizal fungi, significantly increasing the spore production of the arbuscular mycorrhizal fungi. The hairy roots of the gibberellin-deficient mutant can significantly increase the spore production of the arbuscular mycorrhizal fungi under both normal pH conditions and acidic conditions. The gibberellin-deficient mutant can be widely used to promote spore production in arbuscular mycorrhizal fungi. The present invention also provides a preparation and method for promoting spore production in arbuscular mycorrhizal fungi. The preparation and method can propagate the arbuscular mycorrhizal fungi under sterile conditions with high propagation efficiency, significantly increase the spore production of the arbuscular mycorrhizal fungi, are simple to operate, low in cost, and have a short cycle time, thus having broad prospects for promotion and application.

The invention discloses a selenium-rich day lily fertilizer which comprises the following components in percentage by weight: 1.8-2.1 parts of a sodium selenite solution, 5.5-6.5 parts of a fulvic acid solution, 1.4-1.6 parts of a gibberellin 3 solution, 0.4-0.6 part of orange peel essential oil and 980-1000 parts of water, and discloses a preparation method and a use method of the selenium-rich day lily fertilizer. The problem that no fertilizer for selenium-rich day lily exists is solved.

The invention provides klebsiella oxytoca as well as a fungicide and application thereof, and relates to the field of microorganisms. The Klebsiella oxytoca is specifically Klebsiella oxytoca NA-K1, and the preservation number of the Klebsiella oxytoca NA-K1 is CGMCC (China General Microbiological Culture Collection Center) No.32130. The Klebsiella oxytoca NA-K1 can be used for preparing the acid-producing The Klebsiella oxytoca NA-K1 provided by the invention has a relatively comprehensive growth promoting function: the Klebsiella oxytoca NA-K1 can produce heteroauxin at high yield, also has the capability of producing gibberellin, can effectively solubilize phosphorus and potassium, and also has the capability of fixing nitrogen. In addition, the klebsiella oxytoca NA-K1 can resist extreme environments such as acid and alkali, high salt and the like, and is high in stress resistance. Based on a relatively comprehensive growth promoting function and relatively strong stress resistance of the strain, soil nutrients can be increased, the fertilizer utilization rate can be increased, the absorption of plants to the soil nutrients can be promoted, experiments prove that the strain can be successfully planted in rhizosphere soil of cucumbers and spinaches, the growth of the cucumbers and the spinaches can be promoted, the yield can be increased, and the strain has great application potential.

The invention relates to the technical field of rice seed germination accelerating systems and methods. Seed selection is conducted, specifically, rice seeds which are full and free of damage are screened, and impurities are removed; the rice seeds are soaked in a plant growth regulator mixed solution to be subjected to oscillation treatment, the mixed solution is composed of gibberellin with the concentration being 20-50 mg / L, pimacol with the concentration being 5-15 mg / L and polyoxyethylene lauryl ether with the concentration being 0.01-0.03%, the liquid temperature is kept at 30 + / -2 DEG C, and the treatment time is 2-4 hours; gradient disinfection: primary disinfection: putting the pretreated seeds into a 0.3-0.5% hydrogen peroxide solution at the temperature of 40-50 DEG C, and stirring for 15-20 minutes; secondary disinfection: transferring to a 0.8-1.2% sodium hypochlorite solution at the temperature of 25-30 DEG C, adding a 0.05-0.1 mol / L phosphate buffer solution to adjust the pH value to 6.8-7.2, and standing for 40-60 minutes; the disinfected seeds are put into magnetized water containing 0.1%-0.3% of chitosan derivatives and 0.05%-0.15% of sodium alginate, the magnetic field intensity ranges from 300 gauss to 500 gauss, and ultrasonic waves of 30 kHz to 50 kHz are adopted for assisting permeation for 1 h to 2 h; the rice seed germination accelerating system and method are high in germination accelerating efficiency.

The invention belongs to the technical field of plant biology, and particularly relates to a tissue culture medium and a tissue culture method for culturing ginseng stem cells. The invention provides a tissue culture medium and a tissue culture method for culturing ginseng stem cells. The tissue culture medium comprises a germination medium, a growth medium, an induction medium and a proliferation medium. Under the combined action of NAA, 2, 4-D, 6-BA, KT, gibberellin GA3, acid hydrolyzed casein, carbon nanotubes, activated carbon, reduced glutathione and vitamin C, the tissue culture medium provided by the invention is beneficial to induction and proliferation of calluses and further conversion of the calluses into stem cells, so that browning of the calluses is effectively prevented. Results of the embodiment show that the tissue culture medium can induce ginseng seeds to germinate in a short time, the browning rate is 10%, the browning rate is low, a large number of ginseng stem cells can be rapidly obtained, and a foundation is laid for application of the ginseng stem cells.