395 results about "Gibberellin" patented technology

The invention belongs to the technical field of microorganisms, and particularly relates to bacillus mobilis DG-7 and application thereof in inhibiting bolting of angelica sinensis and improving disease resistance of angelica sinensis. The invention provides a strain of bacillus mobilis DG-7, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.31089. The bacillus mobilis DG-7 and the microbial inoculum thereof provided by the invention can play roles in inhibiting early bolting of angelica sinensis and improving disease resistance by increasing the content of plant endogenous melatonin and salicylic acid, reducing the content of abscisic acid, auxin and gibberellin, reducing the content of total phenolic acid and reducing the expression of photoperiod-related genes. In addition, the bacillus mobilis DG-7 does not solubilize phosphorus, so that the promotion effect of high-concentration phosphorus on early bolting is effectively avoided; the laccase activity of the bacillus mobilis DG-7 is high, polyphenol substances can be effectively removed, and the bolting rate can be reduced.

The invention belongs to the technical field of biological agents, and particularly relates to bacillus siamensis DG-39, a microbial agent and application of the bacillus siamensis DG-39 to inhibition of early bolting of angelica sinensis. According to the bacillus siamensis DG-39 and the microbial inoculum containing the bacillus siamensis DG-39, the physiological and biochemical states of angelica sinensis are effectively changed mainly by increasing the content of endogenous melatonin of angelica sinensis, reducing the content of abscisic acid, auxin and gibberellin and promoting circulation and utilization of nitrogen, and the photoperiod of the angelica sinensis can be effectively improved by reducing expression of related genes of the photoperiod. The effect of inhibiting early bolting of the angelica sinensis is achieved. Meanwhile, the bacillus siamensis DG-39 microbial inoculum has the characteristics of fixing nitrogen, not dissolving phosphorus and producing siderophore, and can promote the absorption and utilization of plants on nitrogen, so that the promotion effect of excessive phosphorus on bolting is avoided; meanwhile, the bacillus siamensis DG-39 laccase is high in activity, can remove phenolamine substances for promoting bolting, and is beneficial to reducing the bolting rate.

The invention belongs to the technical field of biological agents, and particularly relates to ginsenoside variant flavobacterium DG-16 and application thereof in relieving continuous cropping obstacles of angelica sinensis and improving the quality of the angelica sinensis. According to the ginsenoside variant flavobacterium DG-16 disclosed by the invention, the growth of angelica sinensis is promoted by increasing the contents of jasmonic acid and cytokinin hormones, reducing the contents of gibberellin and salicylic acid hormones and promoting the circulation and utilization of nitrogen; in addition, the flavobacterium ginsenoside variant DG-16 can significantly increase the chlorophyll content, is beneficial to photosynthesis of the angelica sinensis, effectively changes the physiological and biochemical states of the angelica sinensis, can resist root rot of the angelica sinensis so as to relieve successive cropping obstacles and improve the quality, and has a significant effect.

The invention discloses a method for seed production and seedling breeding of clematis orientalis. The method comprises five key steps of seed shell pretreatment, seed treatment, soil transferring and planting, plant transplanting and seedling culture management. According to the method, seed shells are creatively polished by abrasive paper until the seed shells are thinned but seed kernels are not damaged, and the seed germination time is shortened to 14.08 days and the germination rate is increased to 78.33% in combination with a synergistic treatment technology of soaking in a gibberellin solution. By designing a stepped seedling culture container system and matching with a peat-perlite-vermiculite matrix ratio, accurate matching between root development and container space is realized. By adopting a temperature and humidity intelligent regulation and control and staged water and fertilizer management scheme and combining with precise application of a water-soluble fertilizer and an aoGreen A5 slow-release fertilizer, the survival rate and growth vigor of seedlings are remarkably improved. The method breaks through the traditional breeding bottleneck, shortens the breeding period, improves the seed germination rate, realizes factory-like efficient production of clematis seedlings, and has remarkable economic benefits and ecological values.

The invention discloses a preparation technology of a special cistanche inoculation block with a seed dormancy breaking self-induction function by combining a cistanche seed dormancy breaking inducer and hydrogel, and relates to the technical field of cistanche cultivation. The specific technical process is as follows: preparation of a dormancy breaking inducer solution, preparation of a hydrogel block containing the dormancy breaking inducer and cistanche seeds, and preparation of an inoculation block. According to the method, gibberellin and cytokinin are used as dormancy breaking inducers, and a yeast aqueous extract is used as an auxiliary agent. The problems of seed dormancy breaking, germination promoting and water retention inoculation are effectively solved, seed dormancy breaking does not need to be conducted in advance before inoculation, meanwhile, hydrogel has high water absorption and water retention capacity, the problem that dormancy breaking seeds are inactivated due to the fact that the dormancy breaking seeds cannot germinate and parasitize in time after being inoculated due to water shortage is effectively solved, and the survival rate of the seeds is increased. The inoculation efficiency is improved to 75% or above from about 20% of existing inoculation paper inoculation, and the method has a good popularization prospect and has very important significance in promoting the industrial development of cistanche deserticola.

The invention relates to the field of ultraviolet light absorbers, and discloses a research and development method of an ultraviolet light absorber with a novel structure. Comprising the following raw materials in parts by weight: 25 to 30 parts of pyrene-triazine hybrid, 10 to 20 parts of MOF loaded benzotriazole, 15 to 20 parts of silicon dioxide coated nano zinc oxide, 10 to 15 parts of carbon quantum dot modified titanium dioxide, 8 to 12 parts of PLGA microcapsules, 7 to 13 parts of chitosan-hyaluronic acid composite film, 12 to 18 parts of Eu < 3 + > coated MOF fluorescent material, 8 to 12 parts of gibberellin-nano selenium compound and 5 to 8 parts of ionic liquid PF6. The absorption wavelength range is widened by virtue of a unique structure of a pyrene-triazine hybrid, the stability and dispersity are improved by MOF-loaded benzotriazole through MOF pore channels, the conditions of agglomeration and poor stability of nano-zinc oxide are improved by coating nano-zinc oxide with silicon dioxide, and the photoresponse range of titanium dioxide is expanded by modifying titanium dioxide with carbon quantum dots.

The invention discloses a podophyllum hexandrum callus rapid proliferation method, and belongs to the technical field of plant tissue culture, the podophyllum hexandrum seed dormancy is broken through a sand storage method, the seeds are soaked in distilled water and a gibberellin solution and then are fully and uniformly mixed with river sand, after storage and germination acceleration, the seeds are sown in soil added with an equal amount of matrix, and podophyllum hexandrum seedlings are obtained through culture; fresh leaves of podophyllum hexandrum seedlings are taken as explants, the explants are disinfected and cut into small pieces, the small pieces are cultured on an MS culture medium added with hormone for 30 d to induce callus, the induced callus is cultured and proliferated at the optimal temperature of 15 DEG C, and the callus is inoculated to the same fresh culture medium after being cultured for 20 d to prevent browning, so that the high-quality callus is obtained. And then replacing a fresh culture medium every 20 days to realize rapid and mass proliferation of the podophyllum hexandrum callus. Through the method, a large amount of high-quality podophyllum hexandrum callus can be rapidly obtained, and sufficient materials are provided for podophyllotoxin extraction and medicinal value development.

The invention discloses a cultivation method for improving quality and aroma of flue-cured tobaccos. The cultivation method comprises the steps that 1, the flue-cured tobaccos are planted in rainy tobacco areas in the south; 2, preparing a foliar fertilizer; the leaf fertilizer is prepared from the following components in parts by mass: 1000 parts of tobacco stem leaching liquor, 100 to 500 parts of amino acid raw powder, 50 to 100 parts of potassium nitrate, 50 to 100 parts of monopotassium phosphate, 0.005 to 0.05 part of gibberellin and 100 to 250 parts of glucose; the foliar fertilizer is prepared by adding the components in proportion and fully and uniformly mixing the components. 3, before and after topping of flue-cured tobacco plants, the prepared foliar fertilizer is used for at least one time of foliar spraying operation on fresh tobacco leaves of flue-cured tobacco. According to the quality-improving and aroma-enhancing cultivation method for the flue-cured tobacco, nitrogen, phosphorus and potassium nutrient elements and plant hormones, namely gibberellin and glucose are reasonably added into the leaf fertilizer, so that sufficient growth, natural maturity and chemical component coordination regulation of the tobacco leaves are realized, and the aroma quality of the flue-cured tobacco is improved.

The invention provides a grifola frondosa liquid culture medium as well as a culture method and application thereof, and relates to the technical field of fermentation. The invention provides a grifola frondosa liquid culture medium, which contains glucose, peptone, KH2P2O4, MgSO4, vitamin B1, gibberellin and tyrosol, can significantly improve the growth of grifola frondosa thalli and the secretion of exopolysaccharides, and can be used for the preparation of food, drugs and health care products.

The present invention discloses the use of a gibberellin-deficient mutant in promoting spore production in arbuscular mycorrhizal fungi. The present invention utilizes the hairy roots of the gibberellin-deficient mutant as a host plant to inoculate the arbuscular mycorrhizal fungi, significantly increasing the spore production of the arbuscular mycorrhizal fungi. The hairy roots of the gibberellin-deficient mutant can significantly increase the spore production of the arbuscular mycorrhizal fungi under both normal pH conditions and acidic conditions. The gibberellin-deficient mutant can be widely used to promote spore production in arbuscular mycorrhizal fungi. The present invention also provides a preparation and method for promoting spore production in arbuscular mycorrhizal fungi. The preparation and method can propagate the arbuscular mycorrhizal fungi under sterile conditions with high propagation efficiency, significantly increase the spore production of the arbuscular mycorrhizal fungi, are simple to operate, low in cost, and have a short cycle time, thus having broad prospects for promotion and application.

The invention relates to the technical field of rice seed germination accelerating systems and methods. Seed selection is conducted, specifically, rice seeds which are full and free of damage are screened, and impurities are removed; the rice seeds are soaked in a plant growth regulator mixed solution to be subjected to oscillation treatment, the mixed solution is composed of gibberellin with the concentration being 20-50 mg / L, pimacol with the concentration being 5-15 mg / L and polyoxyethylene lauryl ether with the concentration being 0.01-0.03%, the liquid temperature is kept at 30 + / -2 DEG C, and the treatment time is 2-4 hours; gradient disinfection: primary disinfection: putting the pretreated seeds into a 0.3-0.5% hydrogen peroxide solution at the temperature of 40-50 DEG C, and stirring for 15-20 minutes; secondary disinfection: transferring to a 0.8-1.2% sodium hypochlorite solution at the temperature of 25-30 DEG C, adding a 0.05-0.1 mol / L phosphate buffer solution to adjust the pH value to 6.8-7.2, and standing for 40-60 minutes; the disinfected seeds are put into magnetized water containing 0.1%-0.3% of chitosan derivatives and 0.05%-0.15% of sodium alginate, the magnetic field intensity ranges from 300 gauss to 500 gauss, and ultrasonic waves of 30 kHz to 50 kHz are adopted for assisting permeation for 1 h to 2 h; the rice seed germination accelerating system and method are high in germination accelerating efficiency.

The invention belongs to the technical field of plant seedling raising, and relates to a method for breaking dormancy of iris ensata thunb seeds and accelerating germination, which comprises the following steps: (1) soaking disinfected iris ensata thunb seeds in hydrogen peroxide with the concentration of 3-7wt% for 15-30min; and (2) at 25-30 DEG C, soaking with a mixed solution of gibberellin 3 and indoleacetic acid, and accelerating germination. Hard seed coats are broken through H2O2 soaking treatment, seed coat obstacles and physiological dormancy of the iris ensata thunb seeds are broken through combination of a GA3 + IAA seed soaking mode and fine sand covering germination acceleration, hormones needed by seed germination are supplemented, germination inhibitors existing in the seeds are inhibited, dormancy of the iris ensata thunb seeds is comprehensively relieved, the germination rate of the iris ensata thunb seeds is remarkably increased, and the germination rate of the iris ensata thunb seeds is increased. The germination rate is increased to 80% or above, the germination time can be further shortened, and technical support is provided for large-scale production and application of iris ensata thunb.

The invention relates to the technical field of seed treatment, and provides a pelleting method for alfalfa seeds used in a coastal saline-alkali district, and the alfalfa seeds sequentially comprise seeds, a hormone layer, a first oxygenation breathable layer, a nutrition layer, a second oxygenation breathable layer and an outer protection layer from inside to outside; the raw material of the hormone layer comprises gibberellin; the raw materials of the first oxygen-increasing breathable layer comprise an adhesive and a functional material; the raw material of the second oxygenation breathable layer comprises a functional material; the functional material comprises gypsum and / or calcium peroxide; the raw material of the outer protection layer comprises organic acid. By means of the technical scheme, the problem that the emergence rate of alfalfa seeds is low due to the fact that saline-alkali soil in a saline-alkali area is large in viscosity and poor in air permeability in the prior art is solved.

The invention relates to the technical field of flower flowering phase regulation and control, and discloses a regulator and a method for promoting secondary flowering of crape myrtle, the regulator comprises 50 parts by weight of benzylaminopurine, 4.6-5 parts by weight of compound sodium nitrophenolate and more than or equal to 100 parts by weight of an amino acid composition; or 18 parts by weight of benzylaminopurine, 18 parts by weight of gibberellin, 4.6-5 parts by weight of compound sodium nitrophenolate and more than or equal to 100 parts by weight of an amino acid composition. The regulator provided by the invention comprehensively acts on multiple aspects of cell division, dormancy breaking, hormone regulation, nutrition supply, stress resistance enhancement and the like to jointly promote secondary blooming of the crape myrtle, increase the blooming amount and prolong the blooming period, and by virtue of multiple aspects of effects of pruning, fertilization, insect killing, plant growth regulator regulation and the like, the yield of the crape myrtle is increased. Secondary blooming of the crape myrtle is promoted, the blooming rate, the blooming duration and the blooming amount of secondary blooming are increased, and the growth vigor of the crape myrtle in the next year is not affected.

The invention belongs to the technical field of plant growth regulators, and particularly relates to a plant growth regulator for inhibiting plant germination and application thereof, the plant growth regulator for inhibiting plant germination is obtained by compounding S-abscisic acid and dihydrogibberellin; in the plant growth regulator for inhibiting plant germination, the molar ratio of dihydrogibberellin to S-abscisic acid is (0.2-2): (0.1-1). By combining dihydrogibberellin with S-abscisic acid, the proportion of endogenous hormone gibberellin and abscisic acid in wheat grains can be effectively reduced, the activity of alpha-amylase is reduced, the content of soluble sugar is reduced, the dormancy capacity of the wheat grains is enhanced, and the germination rate of the wheat grains in the mature period is remarkably reduced.

The invention discloses a bletilla striata seedling raising method which comprises the following steps: selecting and pretreating seeds, selecting healthy and mature bletilla striata seeds, ensuring that the seeds are free of plant diseases and insect pests and seed coats are complete, soaking the screened mature seeds in warm water of 30 DEG C for 4 hours, promoting the seed coats to be softened, breaking seed dormancy, and raising the seedlings. Transferring the soaked seeds into a low-temperature environment of 4 DEG C for stratification treatment, wherein the stratification time is 15 days. A stratification medium is wet vermiculite, the humidity is kept at about 60%, and after stratification treatment, the seeds are soaked in a 0.05% gibberellin solution for 30 minutes to promote germination of germs. The invention relates to the technical field of plant seedling cultivation, in particular to a bletilla striata seedling cultivation method capable of designing differentiated pretreatment processes according to different propagation materials, achieving accurate management of temperature and humidity, illumination and fertility and improving the bletilla striata seedling cultivation effect.

The present invention relates to the field of allergens and more specifically to a novel isolated allergen from the species Cupressaceae shown to be a primary sensitizer for severe peach allergy mediated by the protein Pru p7. The allergen is homologous with Pru P7 in peach, a protein belonging to the Gibberelin Regulating Proteins (GRPs). The novel allergen also 5 shares high sequence homology among other species from the Cupressaceae family and there is therefore also provided additional novel allergens in two other species of the Cupressaceae family. The novel allergens find use in the in vitro diagnosis of, treatment and / or prevention of Type 1 Cupressaceace pollen allergy and Cupressaceace pollen-associated food allergies.

The invention discloses a method for adjusting the pore opening degree of Shanghai green, and belongs to the technical field of plant detection. Shanghai leaves are placed in 20mL of open liquid containing (1) 0.5% potassium chloride and 100 [mu] M / L calcium chloride or (2) 0.5% potassium chloride and 50 [mu] M / L brassinolide or (3) 0.5% potassium chloride and 10 [mu] M / L cytokinin or (4) 0.5% potassium chloride and 5 [mu] M / L gibberellin or (5) 0.5% potassium chloride and 100 [mu] M / L methyl jasmonate, culture is performed under illumination, and the illumination time is set to be 5h, so that the pore opening degree of Shanghai is remarkably increased. Compared with a control group (containing 0.5% of potassium chloride), the pore openness of the treatment is respectively increased by 33.5%, 43.5%, 43.4%, 27.1% and 70.2%. The method disclosed by the invention lays a technical foundation for rapidly screening out the exogenous environmental stimulation factor (environical stimuli) capable of increasing the opening degree of the pore.

The present invention discloses a method for recrystallization purification of gibberellin GA7, which relates to the field of production and extraction of plant growth regulators. In this method, the crude gibberellin GA7 is dissolved in ethanol, and after filtration, a gibberellin GA7 solution is obtained. The GA7 solution is decolorized with activated carbon and then filtered to obtain a decolorized gibberellin GA7 solution. After adjusting the pH of the decolorized gibberellin GA7 solution with hydrochloric acid, deionized water is slowly added until the fine powder of gibberellin GA7 no longer precipitates. After standing, filtration and drying are carried out. The mother liquor contains almost no gibberellin GA7. At the same time, after the mother liquor recovers ethanol, it is basically all water and does not require environmental protection treatment. This method is simple to operate, safe and environmentally friendly, has a short process flow, is easy to implement, and is suitable for industrial production.

The invention belongs to the technical field of plant cultivation, and particularly relates to a method for promoting vernalization of rape by using gibberellin. The invention discloses a method for promoting vernalization of winter rape by utilizing gibberellin, which comprises the following steps: (1) performing constant-temperature light-shielding germination treatment on brassica napus seed SC4 in gibberellin to obtain the brassica napus seed SC4 with white buds; and (2) culturing the brassica napus seed (Brassica napus seed) SC4 with the white buds under the condition of long sunshine. Under the long-day illumination condition, the flowering periods of the cabbage type rape seeds SC4 which are not vernalized are treated with the gibberellin concentration of 0.001 mol / L and 0.002 mol / L, the flowering periods are extremely remarkable, and the flowering periods of the materials are remarkably shortened.

The present invention relates to the technical field of seed coating agents, and particularly relates to a drought-resistant and saline-alkali-tolerant corn seed coating agent and a preparation method thereof, comprising the following raw materials: nano-polyacrylamide, polyvinyl alcohol, Mo@ZIF-8, modified MgAl-LDH, gibberellin, proline, citric acid, sodium carboxymethyl cellulose, and Bacillus subtilis. In the present invention, Mo@ZIF-8 and modified MgAl-LDH synergistically enhance the drought resistance and saline-alkali tolerance of corn seeds. Mo@ZIF-8 adsorbs Na + to prevent it from entering the seed epidermal cells, alleviating saline-alkali stress. Modified MgAl-LDH forms a hydration layer with water molecules to effectively retain water. The surface-grafted polydopamine helps to achieve dynamic water regulation, enhancing the drought resistance of corn seeds. The catalytic activity of Mo@ZIF-8 is enhanced through the photothermal effect, promoting the oxidation of Cl ‑ to release Cl2. The gradually released imidazole molecules from Mo@ZIF-8 reduce the alkalinity of the rhizosphere environment, improve the seed germination environment, optimize the rhizosphere microbial environment, and promote crop root growth.

The invention discloses a nutrition regulating agent for regulating the mature period of citrus fruits and application of the nutrition regulating agent. The nutrition regulating agent is prepared from potassium nitrate, monopotassium phosphate, magnesium sulfate, ferric sodium ethylenediaminetetraacetate, zinc sodium ethylenediaminetetraacetate, boric acid, ammonium molybdate, gibberellin, abscisic acid and diatomite which is supplemented to 100 parts. The invention further provides a preparation method of the nutrition regulating agent and application of the nutrition regulating agent in regulating the mature period of citrus fruits, and the application mode is that when the diameter of the citrus fruits is 40-50 mm, the preparation is diluted by 600-800 times to be sprayed on leaf surfaces, once every 7 days and twice continuously. Through the synergistic effect of mineral nutrient elements and low-concentration hormones, bidirectional precise regulation and control of the citrus mature period are achieved, fruits can be promoted to mature in advance or later for 7-15 days, the sugar-acid ratio and color of the fruits can be remarkably improved, meanwhile, the problems of tree vigor decline and quality decline caused by single hormone treatment are solved, and the method is suitable for large-scale popularization and application. The method has the characteristics of high efficiency, safety and simple operation.

The invention discloses a seedling raising and transplanting method for mangrove plant Aegiceras corniculatum based on external application of gibberellin, and belongs to the technical field of saline-alkali plant cultivation and mud flat ecological restoration. The method mainly comprises the four stages of seed disinfection and gibberellin soaking, germination cultivation, seedling cultivation, salinity domestication and mud flat transplanting. After being disinfected, the seeds are soaked in a gibberellin solution with the concentration being 300 mg / L, gibberellin with the same concentration is sprayed regularly during germination, and the germination rate and the germination speed are increased. In the seedling stage, humus soil and mud flat bottom mud mixed matrix is adopted for cultivation, and growth is promoted by spraying 100 mg / L gibberellin to leaf surfaces and injecting 25 mg / L gibberellin to rhizosphere. After seedlings are formed, the seedlings are subjected to step-by-step salinity domestication (5 per thousand to 30 per thousand), gibberellin spraying and rhizosphere injection are carried out at regular intervals, and the stress resistance of the seedlings is enhanced. The method can effectively shorten the seedling raising period of Aegiceras corniculatum, improve the seedling raising efficiency, enhance the adaptive capacity of seedlings to the tidal zone environment, and facilitate rapid recovery of a mangrove forest ecosystem.

The invention provides an under-forest ecological allium victorialis planting method and application, and the method comprises the following steps: after gibberellin treatment and low-temperature sand storage germination acceleration of seeds, sowing and seedling raising are performed in an under-forest or sunshelter high bed with the canopy density of 0.3-0.5, and 2-year-old seedlings are cultivated; transplanting in early April or late September according to the line spacing of 15 cm and the plant spacing of 4-6 cm, and cultivating to obtain 3-4-year-old strong seedlings; then a forest stand with the canopy density of 0.5-0.7 is selected, and field planting is conducted according to the line spacing of 20-30 cm and the plant spacing of 15-30 cm; through intertillage weeding, fertilization and pest and disease management, seedlings are harvested by adopting a three-year ring harvesting method or tender leaves are harvested by adopting a leaf breaking method. According to the method, the problems of low emergence rate, unstable yield, uneven quality and the like caused by direct planting in a wild environment are effectively solved, high-quality, stable-yield and efficient production of allium victorialis is realized, and a reliable mode is provided for utilization of northern forests and development of under-forest economy.

The invention discloses saccharomycetes G-1 and application thereof in degradation of gibberellin GA3, the Latin name of the saccharomycetes G-1 is Yarrowia lipolytica (Yarrowia lipolytica), the strain of the saccharomycetes G-1 is preserved in the China General Microbiological Culture Collection Center on December 1, 2024, and the preservation number is CGMCC No.32800. The invention further discloses a preparation method of the saccharomycetes G-1. The yeast G-1 is screened from a gibberellic acid fermentation filter residue organic fertilizer sample, the strain can grow in an inorganic salt culture medium with gibberellin GA3 as a unique carbon source, and the degradation efficiency of GA3 reaches 24% after the strain is cultured for 48 h; the microbial agent prepared from the microbial agent is applied to the composting process of fermentation filter residues, and the result shows that degradation of GA3 is obviously accelerated by adding 3% of the microbial agent to a treatment group. The strain has great application potential in the aspect of promoting harmless treatment of fermentation filter residues. The invention provides a new way for removing residual plant hormone gibberellin GA3 in soil and organic fertilizers, enriches a germplasm resource library of plant hormone degrading bacteria, and has a better application prospect.

The invention provides a daphne giraldii seed composite treatment solution with epimedium polysaccharide as a main effective component and application, and belongs to the technical field of plant seed treatment, the daphne giraldii seed composite treatment solution comprises 100-300 mg / L of gibberellin, 0.1-100 mg / L of epimedium polysaccharide, 15-35 mg / L of 6-benzylamino adenine, 50-150 mg / L of oligosaccharide, 1-30 mg / L of benzothiadiazole, 20-60 mg / L of a bactericide and 0-1 mL / L of a surfactant. According to the application, the daphne giraldii seeds are treated with the daphne giraldii seed composite treating fluid. According to the composite treatment liquid and the treatment method, the germination rate and the germination potential of the seeds can be remarkably improved, mildewing in the stratification period is effectively inhibited, the growth potential of seedlings is enhanced, a standardized and repeatable operation process is provided, and the composite treatment liquid and the treatment method are suitable for large-scale production.

The invention provides a method for improving hybrid breeding efficiency of small-fruit camellia oleifera, and belongs to the technical field of crop breeding. The method comprises the following steps: spraying a 1%-2.5% compound sodium nitrophenolate solution from the early stage of flower bud differentiation of a hybrid male parent to the stage that flower buds begin to expand; spraying a mixed solution of 10-20mg / L gibberellin and 0.1%-0.3% borax at the early bloom stage of the hybrid male parent; male parent pollen is collected for pollination in the full-bloom middle stage. The compound sodium nitrophenolate lays a good foundation for pollen germination and fruit development in the flower bud differentiation and flower development stages, gibberellin promotes pollen germination and pollen tube growth, borax plays an important role in the pollen tube growth and fertilization process and the later fruit development stage, and the compound sodium nitrophenolate, the gibberellin and the borax cooperate with one another to achieve the effect of promoting pollen germination and fruit development. The pollination and fruit development processes of the small-fruit camellia oleifera are jointly promoted, and the hybridization breeding efficiency is improved.

The present application is suitable for the field of molecular biology and biotechnology, and provides a maize gibberellin 13-hydroxylase gene ZmGA13ox1 and application thereof. The maize gibberellin 13-hydroxylase gene ZmGA13ox1 has a nucleotide sequence shown as SEQ ID NO:1 in the sequence listing. The maize gibberellin 13-hydroxylase protein is encoded by the maize gibberellin 13-hydroxylase gene ZmGA13ox1, and has an amino acid sequence shown as SEQ ID NO:2 in the sequence listing. The present application provides the nucleotide sequence of the ZmGA13ox1 gene in the maize inbred line B73 and the amino acid sequence information of the protein encoded thereby, and uses the agrobacterium-mediated transformation technology to introduce a plant expression vector containing the gene into Arabidopsis thaliana, and cultivates a homozygous T3 generation transgenic Arabidopsis thaliana plant. The experimental results show that the overexpression of the ZmGA13ox1 gene in Arabidopsis thaliana has a promoting effect on the root growth.

The invention discloses a germination accelerating method for pendant flower seeds with different flower colors. According to the method disclosed by the invention, the fact that different flower colors of pendant flowers have different sensitivities to different germination accelerating modes is revealed and found for the first time, white pendant flower seeds and red pendant flower seeds are subjected to germination accelerating in a clear water seed soaking manner, so that a relatively good germination effect can be obtained, and pink pendant flower seeds are subjected to germination accelerating in a gibberellin aqueous solution seed soaking manner, so that a relatively good germination effect can be obtained; a good germination effect can be obtained by accelerating germination of the red and white pennycress seeds by soaking the seeds after skin damage, and a good germination effect can be obtained by accelerating germination of the red powder pennycress seeds by soaking the seeds in clear water and then depositing and accelerating germination. Technical support is provided for formulating a personalized scheme in the process of artificially breeding the pendant flowers and improving the seed germination rate and the seedling quality.

The present invention is directed to mixtures comprising 6-benzyladenine, gibberellic acid 4 / 7 and a third compound selected from the group consisting of proline and malic acid. The present invention is further directed to methods of increasing plant, growth, yield and / or quality comprising applying sequentially or concurrently an effective amount of mixtures of the present invention to the plant or an area where the plant will grow.