Liquid fungicide containing micromolecule'microorganism-crop 'interaction signal substance and application thereof

Abstract

The invention discloses application of a'microorganism-crop 'interaction signal substance in preparation of a product, the'microorganism-crop' interaction signal substance is acetoin and 2, 3-butanediol, and the product is used for regulating and controlling a soil microbial community, improving abundance of soil phosphate solubilizing bacteria, improving activity of soil alkaline phosphatase and soil respiration intensity, and improving soil quality. The fertilizer can regulate and control metabolic pathways in crops, increase the contents of cane sugar, galactose, melibiose, dehydroascorbic acid, tryptophan, gibberellin, jasmonic acid, methyl jasmonate and ferulic acid in the crops, improve the growth and stress resistance of the crops, promote the growth of the crops, increase the yield of the crops, prevent and control crop diseases and improve the yield of the crops. The soil alkaline phosphatase activity and the microbial respiration intensity are improved. The product is a liquid fungicide, and comprises a bacterial liquid, a microorganism-crop interaction signal substance, and at least one of amino acid, seaweed essence and humic acid. The liquid fungicide can promote crop growth, improve crop stress resistance and effectively inhibit pathogenic bacteria.

Description

Technical Field

[0001] The present application relates to the field of biotechnology, and specifically to a liquid microbial agent containing a small molecule "microorganism-crop" interaction signal substance and its use, and more specifically to the use of the "microorganism-crop" interaction signal substance, the liquid microbial agent, a method for regulating crop performance, and a method for regulating soil performance. Background Art

[0002] With the rapid development of modern agriculture, crops are facing many challenges in their growth process, such as poor soil, frequent pests and diseases, stress from adverse conditions, etc. Therefore, developing a bio-microbial agent product that can effectively promote crop growth and improve stress resistance is of great significance to ensure the sustainable development of agricultural production.

[0003] At present, there are many types of biological agents on the market, but most of them have problems such as unstable effects and unclear mechanisms of action. In addition, although some products can temporarily increase crop yields, long-term use may lead to an imbalance in the soil microbial community, which in turn has a negative impact on the ecological environment.

[0004] Therefore, developing a new liquid microbial agent product that can not only promote crop growth and improve stress resistance, but also maintain the balance of soil microbial communities has become a research hotspot in the current field of agricultural biotechnology. Summary of the invention

[0005] The present application aims to solve at least one of the technical problems existing in the prior art to a certain extent. To this end, the present application provides a liquid microbial agent containing a small molecule "microorganism-crop" interaction signal substance, which can promote crop growth, improve crop resistance to adversity, and effectively regulate the structure of soil microbial communities and improve soil health.

[0006] This application is completed based on the following findings of the inventors:

[0007] "Microbe-crop" interaction signal substances are small molecule volatile substances, which are "microbe-crop" signal substances produced by microorganisms. They can promote crop growth and improve crop resistance. Among them, acetoin in the "microbe-crop" interaction signal substances can mainly promote growth and enhance resistance. Many microorganisms can convert sugars into acetoin during glycolysis to avoid excessive acidification. Acetoin can also regulate the ratio of NAD+ / NADH and store carbon sources. In agricultural ecosystems, acetoin, as a type of volatile substance released by root-promoting bacteria, has the effect of promoting plant growth. In addition, acetoin can also be used as an inducer to induce plant systemic resistance to pathogens and protect plants from the harm of some diseases. Studies have found that acetoin, as an important component of the volatile components released by Bacillus, has a good inhibitory effect on post-harvest corruption of citrus. At the same time, acetoin is also an insect pheromone, which can be added to pest killing devices as an insect attractant to effectively reduce crop pests and ensure crop yields. 2,3-Butanediol, a signal substance in the "microbe-crop" interaction, can be used as a growth regulator in crops. By adjusting the balance of plant growth hormones, 2,3-Butanediol can promote the growth of plant roots, improve crop drought and disease resistance, and increase photosynthesis efficiency, thereby promoting the overall growth and development of crops.

[0008] The inventors unexpectedly discovered that small molecule "microbe-crop" interaction signal substances can regulate soil microbial communities, especially increase the relative abundance of species such as Flavobacterium, Polaromonans, Lysobacter, Pseudomonas, Aminobacter, Sphingopyxis, Ensifer, and Sinorhizobium in rhizosphere soil. Further analysis found that small molecule "microbe-crop" signal substances can also promote the relative abundance of genes nirK, nosZ, narH / nxrB and napA involved in denitrification regulation in soil microorganisms, promote the relative abundance of genes gltB, gdhA and glnA / GLUL involved in organic nitrogen metabolism in soil microorganisms, promote the relative abundance of regulatory genes nirB and nasA for soil microbial dissimilation / assimilation of nitrate reduction to ammonia, and significantly increase the relative abundance of regulatory genes nrtA / cynA and nrtC / nasD for nitrate assimilation / nitrogen transport in soil microorganisms. It can regulate rhizosphere microorganisms (mainly reflected in the positive regulation of soil organic phosphorus mineralization and phosphorus transport capacity) and increase soil alkaline phosphatase activity and soil respiration intensity.

[0009] In addition, the inventors unexpectedly discovered that small molecule "microbe-crop" interaction signal substances can target and regulate Arabidopsis-related growth-promoting genes, target and regulate the physiological metabolic pathways of dwarf tomatoes, and increase the content of metabolites in crops.

[0010] Based on this, in the first aspect of the present application, the present application proposes a use of a "microorganism-crop" interaction signal substance in the preparation of a product, wherein the "microorganism-crop" interaction signal substance includes acetoin and 2,3-butanediol, and the product is used to: regulate soil microbial communities, and / or increase the abundance of soil phosphate-solubilizing bacteria, and / or increase soil phosphatase activity and soil respiration intensity, and / or regulate metabolic pathways in crops, and / or increase the content of sucrose, galactose, melibiose, dehydroascorbic acid, tryptophan, gibberellin, jasmonic acid, methyl jasmonate, and ferulic acid in crops, and / or improve crop growth and stress resistance, and / or promote crop growth, and / or increase crop yield, and / or prevent and control crop diseases. Therefore, the product prepared using the "microorganism-crop" interaction signal substance can promote crop growth, improve crop stress resistance, and effectively inhibit pathogens.

[0011] In the second aspect of the present application, the present application proposes a liquid bacterial agent. According to an embodiment of the present application, the liquid bacterial agent includes a bacterial liquid, a "microorganism-crop" interaction signal substance, and at least one of amino acids, seaweed essence, and humic acid. The liquid bacterial agent of the present application can promote crop growth, improve crop resistance, and effectively inhibit pathogens.

[0012] In a third aspect of the present application, the present application proposes a liquid bacterial agent. According to an embodiment of the present application, the liquid bacterial agent is selected from any one of the following groups:

[0013] 1) The liquid bacterial agent comprises bacterial liquid, "microorganism-crop" interaction signal substance and seaweed essence, and the weight ratio of the bacterial liquid, "microorganism-crop" interaction signal substance and seaweed essence is (30-70): (10-20): (1-4);

[0014] The microorganism in the bacterial solution is Bacillus Velez, and the number of viable bacteria of Bacillus Velez in the bacterial solution is 15-30 billion / mL.

[0015] The "microorganism-crop" interaction signal substance includes acetoin and 2,3-butanediol. In the "microorganism-crop" interaction signal substance, the concentration of acetoin is 30 g / L to 60 g / L, and the concentration of 2,3-butanediol is 10 g / L to 30 g / L.

[0016] The mass percentage of alginic acid in the seaweed essence is 20% to 40%;

[0017] 2) The liquid bacterial agent comprises bacterial liquid, "microorganism-crop" interaction signal substance, seaweed essence and composite amino acid, and the weight ratio of the bacterial liquid, "microorganism-crop" interaction signal substance, seaweed essence and composite amino acid is (30-70): (10-20): (0.5-2): (0.5-2);

[0018] The microorganism in the bacterial solution is Bacillus subtilis, and the number of viable bacteria of Bacillus subtilis in the bacterial solution is 15-25 billion / mL.

[0019] The "microorganism-crop" interaction signal substance includes acetoin and 2,3-butanediol. In the "microorganism-crop" interaction signal substance, the concentration of acetoin is 30 g / L to 60 g / L, and the concentration of 2,3-butanediol is 10 g / L to 30 g / L.

[0020] The mass percentage of alginic acid in the seaweed essence is 20% to 40%.

[0021] The mass percentage of free amino acids in the composite amino acids is 20% to 30%;

[0022] 3) The liquid bacterial agent comprises bacterial liquid, "microorganism-crop" interaction signal substance and humic acid, and the weight ratio of the bacterial liquid, "microorganism-crop" interaction signal substance and humic acid is (30-70): (10-20): (4-6);

[0023] The microorganism in the bacterial solution is Bacillus megaterium, and the number of viable bacteria of Bacillus megaterium in the bacterial solution is 5-10 billion / mL.

[0024] The "microorganism-crop" interaction signal substance includes acetoin and 2,3-butanediol. In the "microorganism-crop" interaction signal substance, the concentration of acetoin is 30 g / L to 60 g / L, and the concentration of 2,3-butanediol is 10 g / L to 30 g / L.

[0025] The mass percentage of fulvic acid in the humic acid is 20% to 40%;

[0026] 4) The liquid bacterial agent comprises bacterial liquid, "microorganism-crop" interaction signal substance, seaweed essence and composite amino acid, and the weight ratio of the bacterial liquid, "microorganism-crop" interaction signal substance, seaweed essence and composite amino acid is (30-70), (10-20), (2-3), (2-3);

[0027] The microorganisms in the bacterial solution are Bacillus subtilis and Bacillus megaterium, the number of live bacteria of Bacillus subtilis in the bacterial solution is 10-20 billion / mL, and the number of live bacteria of Bacillus megaterium in the bacterial solution is 100-100 million / mL.

[0028] The "microorganism-crop" interaction signal substance includes acetoin and 2,3-butanediol. In the "microorganism-crop" interaction signal substance, the concentration of acetoin is 30 g / L to 60 g / L, and the concentration of 2,3-butanediol is 10 g / L to 30 g / L.

[0029] The mass percentage of alginic acid in the seaweed essence is 20% to 40%.

[0030] The mass percentage of free amino acids in the composite amino acids is 20% to 30%;

[0031] 5) The liquid bacterial agent comprises bacterial liquid, "microorganism-crop" interaction signal substance and seaweed essence, and the weight ratio of the bacterial liquid, "microorganism-crop" interaction signal substance and seaweed essence is (30-70): (10-20): (1-2);

[0032] The microorganisms in the bacterial solution are Bacillus Velez and Bacillus mucilaginosus, the number of live bacteria of Bacillus Velez in the bacterial solution is 10-20 billion / mL, and the number of live bacteria of Bacillus mucilaginosus in the bacterial solution is 5-2 billion / mL.

[0033] The "microorganism-crop" interaction signal substance includes acetoin and 2,3-butanediol. In the "microorganism-crop" interaction signal substance, the concentration of acetoin is 30 g / L to 60 g / L, and the concentration of 2,3-butanediol is 10 g / L to 30 g / L.

[0034] The mass percentage of alginic acid in the seaweed essence is 20% to 40%;

[0035] 6) The liquid bacterial agent comprises bacterial liquid, "microorganism-crop" interaction signal substance and humic acid, and the weight ratio of the bacterial liquid, "microorganism-crop" interaction signal substance and humic acid is (30-70): (10-20): (4-6);

[0036] The microorganisms in the bacterial solution are Bacillus subtilis, Bacillus megaterium and Bacillus colloids. The number of live bacteria of Bacillus subtilis in the bacterial solution is 5-10 billion / mL, the number of live bacteria of Bacillus megaterium in the bacterial solution is 5-2 billion / mL, and the number of live bacteria of Bacillus colloids in the bacterial solution is 1-1 billion / mL.

[0037] The "microorganism-crop" interaction signal substance includes acetoin and 2,3-butanediol. In the "microorganism-crop" interaction signal substance, the concentration of acetoin is 30 g / L to 60 g / L, and the concentration of 2,3-butanediol is 10 g / L to 30 g / L.

[0038] The mass percentage of fulvic acid in the humic acid is 20% to 40%;

[0039] 7) The liquid bacterial agent comprises bacterial liquid, "microorganism-crop" interaction signal substance and seaweed essence, and the weight ratio of the bacterial liquid, "microorganism-crop" interaction signal substance and seaweed essence is (30-70): (10-20): (1-2);

[0040] The microorganisms in the bacterial solution are Bacillus Velez, Bacillus mucilaginosus and Bacillus laterosporus. The number of live bacteria of Bacillus Velez in the bacterial solution is 5-20 billion / mL, the number of live bacteria of Bacillus mucilaginosus in the bacterial solution is 100-500 million / mL, and the number of live bacteria of Bacillus laterosporus in the bacterial solution is 10-20 billion / mL.

[0041] The "microorganism-crop" interaction signal substance includes acetoin and 2,3-butanediol. In the "microorganism-crop" interaction signal substance, the concentration of acetoin is 30 g / L to 60 g / L, and the concentration of 2,3-butanediol is 10 g / L to 30 g / L.

[0042] The mass percentage of alginic acid in the seaweed essence is 20% to 40%.

[0043] The above-mentioned liquid bacterial agent of the present application can effectively promote crop growth and effectively regulate soil microecology, providing strong technical support for the sustainable development of modern agriculture.

[0044] In a fourth aspect of the present application, the present application proposes a liquid bacterial agent. According to an embodiment of the present application, the liquid bacterial agent is selected from any one of the following groups:

[0045] 1) The liquid bacterial agent comprises bacterial liquid, "microorganism-crop" interaction signal substance and seaweed essence, and the weight ratio of the bacterial liquid, "microorganism-crop" interaction signal substance and seaweed essence is (55-65): (10-20): 4;

[0046] The microorganism in the bacterial solution is Bacillus Velez, and the number of viable bacteria of Bacillus Velez in the bacterial solution is 20-30 billion / mL.

[0047] The "microorganism-crop" interaction signal substance includes acetoin and 2,3-butanediol. In the "microorganism-crop" interaction signal substance, the concentration of acetoin is 40g / L to 60g / L, and the concentration of 2,3-butanediol is 10g / L to 30g / L.

[0048] The mass percentage of alginic acid in the seaweed essence is 20% to 40%;

[0049] 2) The liquid bacterial agent comprises bacterial liquid, "microorganism-crop" interaction signal substance, seaweed essence and composite amino acid, and the weight ratio of the bacterial liquid, "microorganism-crop" interaction signal substance, seaweed essence and composite amino acid is (60-70): (10-20): 0.5: 0.5;

[0050] The microorganism in the bacterial solution is Bacillus subtilis, and the number of viable bacteria of Bacillus subtilis in the bacterial solution is 15-25 billion / mL.

[0051] The "microorganism-crop" interaction signal substance includes acetoin and 2,3-butanediol. In the "microorganism-crop" interaction signal substance, the concentration of acetoin is 30 g / L to 50 g / L, and the concentration of 2,3-butanediol is 10 g / L to 30 g / L.

[0052] The mass percentage of alginic acid in the seaweed essence is 20% to 40%.

[0053] The mass percentage of free amino acids in the composite amino acids is 20% to 30%;

[0054] 3) The liquid bacterial agent comprises bacterial liquid, "microorganism-crop" interaction signal substance and humic acid, and the weight ratio of the bacterial liquid, "microorganism-crop" interaction signal substance and humic acid is (30-40): (10-20): 4;

[0055] The microorganism in the bacterial solution is Bacillus megaterium, and the number of viable bacteria of Bacillus megaterium in the bacterial solution is 5-10 billion / mL.

[0056] The "microorganism-crop" interaction signal substance includes acetoin and 2,3-butanediol. In the "microorganism-crop" interaction signal substance, the concentration of acetoin is 40g / L to 60g / L, and the concentration of 2,3-butanediol is 10g / L to 30g / L.

[0057] The mass percentage of fulvic acid in the humic acid is 20% to 40%;

[0058] 4) The liquid bacterial agent comprises bacterial liquid, "microorganism-crop" interaction signal substance, seaweed essence and composite amino acid, and the weight ratio of the bacterial liquid, "microorganism-crop" interaction signal substance, seaweed essence and composite amino acid is (45-55), (10-20), 2.5, 2.5;

[0059] The microorganisms in the bacterial solution are Bacillus subtilis and Bacillus megaterium, the number of live bacteria of Bacillus subtilis in the bacterial solution is 10-20 billion / mL, and the number of live bacteria of Bacillus megaterium in the bacterial solution is 100-100 million / mL.

[0060] The "microorganism-crop" interaction signal substance includes acetoin and 2,3-butanediol. In the "microorganism-crop" interaction signal substance, the concentration of acetoin is 30 g / L to 50 g / L, and the concentration of 2,3-butanediol is 10 g / L to 20 g / L.

[0061] The mass percentage of alginic acid in the seaweed essence is 20% to 40%.

[0062] The mass percentage of free amino acids in the composite amino acids is 20% to 30%;

[0063] 5) The liquid bacterial agent comprises bacterial liquid, "microorganism-crop" interaction signal substance and seaweed essence, and the weight ratio of the bacterial liquid, "microorganism-crop" interaction signal substance and seaweed essence is (60-70): (10-20): 1;

[0064] The microorganisms in the bacterial solution are Bacillus Velez and Bacillus mucilaginosus, the number of live bacteria of Bacillus Velez in the bacterial solution is 10-20 billion / mL, and the number of live bacteria of Bacillus mucilaginosus in the bacterial solution is 500-1500 million / mL.

[0065] The "microorganism-crop" interaction signal substance includes acetoin and 2,3-butanediol. In the "microorganism-crop" interaction signal substance, the concentration of acetoin is 30 g / L to 40 g / L, and the concentration of 2,3-butanediol is 20 g / L to 30 g / L.

[0066] The mass percentage of alginic acid in the seaweed essence is 20% to 40%;

[0067] 6) The liquid bacterial agent comprises bacterial liquid, "microorganism-crop" interaction signal substance and humic acid: the weight ratio of the bacterial liquid, "microorganism-crop" interaction signal substance and humic acid is (40-60): (10-20): 6;

[0068] The microorganisms in the bacterial solution are Bacillus subtilis, Bacillus megaterium and Bacillus colloids, the number of live bacteria of Bacillus subtilis in the bacterial solution is 5-10 billion / mL, the number of live bacteria of Bacillus megaterium in the bacterial solution is 500-1500 million / mL, and the number of live bacteria of Bacillus colloids in the bacterial solution is 100-500 million / mL.

[0069] The "microorganism-crop" interaction signal substance includes acetoin and 2,3-butanediol. In the "microorganism-crop" interaction signal substance, the concentration of acetoin is 50 g / L to 60 g / L, and the concentration of 2,3-butanediol is 20 g / L to 30 g / L.

[0070] The mass percentage of fulvic acid in the humic acid is 20% to 40%;

[0071] 7) The liquid bacterial agent comprises bacterial liquid, "microorganism-crop" interaction signal substance and seaweed essence, and the weight ratio of the bacterial liquid, "microorganism-crop" interaction signal substance and seaweed essence is (30-40): (10-20): 1;

[0072] The microorganisms in the bacterial solution are Bacillus Velez, Bacillus mucilaginosus and Bacillus laterosporus. The number of live bacteria of Bacillus Velez in the bacterial solution is 5-15 billion / mL, the number of live bacteria of Bacillus mucilaginosus in the bacterial solution is 100-500 million / mL, and the number of live bacteria of Bacillus laterosporus in the bacterial solution is 100-150 million / mL.

[0073] The "microorganism-crop" interaction signal substance includes acetoin and 2,3-butanediol. In the "microorganism-crop" interaction signal substance, the concentration of acetoin is 30 g / L to 40 g / L, and the concentration of 2,3-butanediol is 10 g / L to 20 g / L.

[0074] The mass percentage of alginic acid in the seaweed essence is 20% to 40%.

[0075] The above-mentioned liquid bacterial agent of the present application can effectively promote crop growth and effectively regulate soil microecology, providing strong technical support for the sustainable development of modern agriculture.

[0076] In the fifth aspect of the present application, the present application proposes a method for regulating crop performance. According to an embodiment of the present application, the method comprises: contacting the crop with a "microorganism-crop" interaction signal substance, the liquid microbial agent described in the second aspect, or the liquid microbial agent described in the third aspect or the fourth aspect; wherein regulating crop performance comprises at least one of the following: regulating the metabolic pathway in the crop, increasing the content of sucrose, galactose, melibiose, dehydroascorbic acid, tryptophan, gibberellin, jasmonic acid, methyl jasmonate, and ferulic acid in the crop, improving the growth and stress resistance of the crop, promoting the growth of the crop, increasing the yield of the crop, and preventing and controlling crop diseases.

[0077] In the sixth aspect of the present application, the present application proposes a method for regulating soil properties. According to an embodiment of the present application, the method comprises: contacting the soil with a "microorganism-crop" interaction signal substance, the liquid bacterial agent described in the second aspect, or the liquid bacterial agent described in the third aspect or the fourth aspect; wherein regulating soil properties comprises at least one of the following: regulating the microbial community in the soil, increasing the abundance of soil phosphate-solubilizing bacteria, increasing the activity of soil alkaline phosphatase, and increasing the respiration intensity of microorganisms in the soil.

[0078] Additional aspects and advantages of the present application will be given in part in the description below, and in part will become apparent from the description below, or will be learned through the practice of the present application. BRIEF DESCRIPTION OF THE DRAWINGS

[0079] The above and / or additional aspects and advantages of the present application will become apparent and easily understood from the description of the embodiments in conjunction with the following drawings, in which:

[0080] Figure 1 The BAR diagram of soil species composition in the data analysis of Example 1 of the present application;

[0081] Figure 2 The relative abundance of genes involved in denitrification regulation of soil microorganisms in the data analysis of Example 1 of the present application;

[0082] Figure 3 The relative abundance of genes involved in organic nitrogen metabolism of soil microorganisms in the data analysis of Example 1 of the present application;

[0083] Figure 4 The relative abundance of genes involved in the regulation of dissimilatory / assimilatory nitrate reduction to ammonia reaction in soil microorganisms in the data analysis of Example 1 of the present application;

[0084] Figure 5 The relative abundance of genes involved in nitrate assimilation / nitrogen transport regulation of soil microorganisms in the data analysis of Example 1 of the present application;

[0085] Figure 6 The relative abundance of soil microorganisms involved in phosphorus stress regulation genes in the data analysis of Example 1 of the present application;

[0086] Figure 7 The relative abundance of soil microbial genes involved in phosphorus absorption and transport in the data analysis of Example 1 of the present application;

[0087] Figure 8 The relative abundance of the soil microbial genes involved in inorganic phosphorus solubilization and organic phosphorus mineralization in the data analysis of Example 1 of the present application;

[0088] Fig. 9 This is a heat map of the correlation between the two matrices of soil microorganisms and N and P related genes in the data analysis of Example 1 of the present application;

[0089] Fig.10 The data analysis of soil respiration intensity and alkaline phosphatase activity in the data analysis of Example 1 of the present application;

[0090] Fig.11 This is the relative abundance of Arabidopsis thaliana targeted genes in the data analysis of Example 2 of the present application. DETAILED DESCRIPTION

[0091] The embodiments of the present application are described in detail below. The embodiments described below are exemplary and are only used to explain the present application, and cannot be understood as limiting the present application.

[0092] It should be noted that the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features. Further, in the description of this application, unless otherwise specified, the meaning of "plurality" is two or more.

[0093] This application details

[0094] Definitions and general terms

[0095] In this document, the terms "include" or "comprising" are open expressions, that is, including the contents specified in the present application but not excluding other contents.

[0096] As used herein, the terms "optionally", "optional", "optionally", "optional" or "optional" generally mean that the subsequently described event or circumstance may but need not occur, and that the description includes instances where the event or circumstance occurs and instances where it does not occur.

[0097] In this article, the term "microorganism-crop interaction signal substance" refers to the chemical signal released when microorganisms and crops communicate. These signal substances can be small molecule compounds, polypeptides, proteins, RNA or combinations thereof. They play a key role in the interaction between microorganisms and crops, affecting the growth, development, disease resistance and adaptability of crops to the environment. Exemplarily, the "microorganism-crop" interaction signal substance is selected from acetoin and 2,3-butanediol.

[0098] In this article, the term "crop" refers to plants grown and harvested through agricultural activities. They are the main objects of agricultural production and are used to provide food, feed, industrial raw materials or ornamental purposes. Including but not limited to: food crops (such as wheat, rice), cash crops (such as cotton, tobacco), feed crops (such as corn), vegetable crops, fruit tree crops, etc.

[0099] In this article, the term "microbial agent" refers to a biological agent that contains one or more beneficial microorganisms, which can be a liquid microbial agent or a solid microbial agent. In this article, microbial agents include but are not limited to biofertilizer microbial agents, biocontrol microbial agents, biostimulant microbial agents, and composite microbial agents. Detailed description of the liquid microbial agent containing small molecule "microorganism-crop" interaction signal substances and its use in this application

[0100] The present application proposes a use of a "microorganism-crop" interaction signal substance, a liquid bacterial agent, a method for regulating crop performance, and a method for regulating soil performance, which will be described in detail below.

[0101] use

[0102] In the first aspect of the present application, the present application proposes a use of a "microorganism-crop" interaction signal substance in the preparation of a product, wherein the "microorganism-crop" interaction signal substance includes acetoin and 2,3-butanediol, and the product is used to: regulate soil microbial communities, and / or increase the abundance of soil phosphate-solubilizing bacteria, and / or increase the activity of soil alkaline phosphatase and soil respiration intensity, and / or regulate metabolic pathways in crops, and / or increase the content of sucrose, galactose, melibiose, dehydroascorbic acid, tryptophan, gibberellin, jasmonic acid, methyl jasmonate, and ferulic acid in crops, and / or improve the growth and stress resistance of crops, and / or promote the growth of crops, and / or increase the yield of crops, and / or prevent and control crop diseases. The inventors of the present application unexpectedly discovered the above functions of "microorganism-crop" interaction signal substances. Therefore, products prepared using "microorganism-crop" interaction signal substances can promote crop growth, improve crop stress resistance, and effectively inhibit pathogens.

[0103] According to an embodiment of the present application, the above-mentioned use may further include at least one of the following technical features:

[0104] According to an embodiment of the present application, the “microorganism-crop” interaction signal substance includes acetoin and 2,3-butanediol. Therefore, the "microbe-crop" interaction signal substance can regulate soil microbial communities, promote the relative abundance of genes nirK, nosZ, narH / nxrB and napA involved in denitrification regulation in soil microorganisms, promote the relative abundance of genes gltB, gdhA and glnA / GLUL involved in organic nitrogen metabolism in soil microorganisms, promote the relative abundance of regulatory genes nirB and nasA for soil microbial dissimilation / assimilation of nitrate reduction to ammonia, and significantly increase the relative abundance of regulatory genes nrtA / cynA and nrtC / nasD for nitrate assimilation / nitrogen transport in soil microorganisms. It can regulate rhizosphere microorganisms (mainly reflected in the positive regulation of soil organic phosphorus mineralization and phosphorus transport capacity), increase soil alkaline phosphatase activity and soil respiration intensity, target the regulation of Arabidopsis-related growth-promoting genes, and target the regulation of dwarf tomato physiological metabolic pathways and increase the content of metabolites in crops.

[0105] According to an embodiment of the present application, the acetoin is selected from D-(-)-acetoin and / or L-(+)-acetoin.

[0106] According to an embodiment of the present application, the acetoin is selected from a mixture of D-(-)-acetoin and L-(+)-acetoin.

[0107] According to an embodiment of the present application, in the “microorganism-crop” interaction signal substance, the mass ratio of acetoin to 2,3-butanediol is (30-60): (10-30).

[0108] According to an embodiment of the present application, in the “microorganism-crop” interaction signal substance, the concentration of acetoin is 30 g / L to 60 g / L, and the concentration of 2,3-butanediol is 10 g / L to 30 g / L.

[0109] According to an embodiment of the present application, the phosphate-solubilizing bacteria include Flavobacterium, Lysobacterium, and Aminobacterium.

[0110] According to an embodiment of the present application, the metabolic pathway includes galactose metabolism, ascorbic acid and aldehyde acid metabolism, tryptophan metabolism, diterpenoid biosynthesis, linolenic acid metabolism, and phenylpropanoid biosynthesis.

[0111] According to an embodiment of the present application, the product is a liquid product or a solid product.

[0112] According to an embodiment of the present application, the product is a bacterial liquid product.

[0113] According to the embodiments of the present application, the product is a liquid bacterial solution. Therefore, the use of a bacterial solution containing the above-mentioned "microorganism-crop" interaction signal substances can adjust the types of microorganisms, utilize their growth promotion, biological control, phosphate solubilization and other functions to promote crop growth, reduce the occurrence of crop diseases, and improve the nutrient absorption and utilization efficiency of crops.

[0114] Liquid inoculant

[0115] In the second aspect of the present application, the present application proposes a liquid bacterial agent. According to an embodiment of the present application, the liquid bacterial agent includes a bacterial liquid, a "microorganism-crop" interaction signal substance, and at least one of amino acids, seaweed essence, and humic acid. The bacterial liquid in the present application can promote crop growth, reduce the occurrence of crop diseases, and improve the nutrient absorption and utilization efficiency of crops by utilizing its growth promotion, biological control, and phosphorus solubilization functions; natural products such as amino acids, seaweed essence, and humic acid can effectively promote the stress resistance of crops and promote crop growth, thereby resisting external adversity and improving crop yield and quality. Moreover, as can be seen from the above, the "microorganism-crop" interaction signal substance has the functions of regulating soil microbial communities, improving crop alkaline phosphatase activity and soil respiration intensity, regulating metabolic pathways in crops, increasing the content of sucrose, galactose, melibiose, dehydroascorbic acid, tryptophan, gibberellin, jasmonic acid, methyl jasmonate, and ferulic acid in crops, improving crop growth and stress resistance, promoting crop growth, increasing crop yields, preventing and controlling crop diseases, and increasing soil alkaline phosphatase activity and microbial respiration intensity. Therefore, the liquid bacterial agent of the present application can promote crop growth, improve crop stress resistance, and effectively inhibit pathogens.

[0116] According to an embodiment of the present application, the liquid bacterial agent may further include at least one of the following technical features:

[0117] According to an embodiment of the present application, the bacterial liquid includes at least one of Bacillus subtilis, Bacillus velez, Bacillus megaterium, Bacillus mucilaginosus, and Bacillus laterosporus.

[0118] The functions of Bacillus subtilis include: inhibiting pathogens, multiplying rapidly in the soil, effectively inhibiting the growth of other pathogens, and preventing soil-borne diseases; improving the soil, decomposing soil organic matter, increasing effective nutrients, improving soil structure, and preventing compaction; promoting growth, producing hormone substances and vitamins, promoting crop growth, and improving yield and quality.

[0119] The functions of Bacillus Velez include: inhibiting pathogens, effectively inhibiting a variety of plant pathogens, and preventing and controlling diseases by secreting antibacterial active metabolites; promoting growth, secreting plant hormones and volatile compounds, and promoting the growth of a variety of crops.

[0120] The functions of Bacillus megaterium include: phosphorus solubilization, converting organic phosphorus into soluble phosphorus to promote plant absorption; antibacterial effect, producing antibacterial substances to prevent and control bacterial and fungal diseases; growth promotion and degradation, producing plant hormones and physiologically active substances to promote crop growth; at the same time, it can degrade harmful substances and purify water.

[0121] The functions of Bacillus subtilis include: decomposing minerals, decomposing potassium and silicon in minerals such as feldspar and mica and phosphorus in apatite to provide nutrients for crops; improving soil and fertilizer utilization, promoting the conversion of ineffective phosphorus and potassium in the soil, and improving fertilizer utilization.

[0122] The functions of Bacillus laterosporus include: inhibiting pathogens, secreting antibiotics, inhibiting the reproduction of harmful bacteria, and reducing crop diseases; promoting root growth, secreting active substances, promoting crop root growth, and improving absorption capacity.

[0123] According to an embodiment of the present application, the microorganism in the bacterial liquid is selected from Bacillus subtilis, Bacillus velez, Bacillus megaterium, Bacillus mucilaginosus, or Bacillus laterosporus.

[0124] According to an embodiment of the present application, the microorganisms in the bacterial liquid are selected from at least three of Bacillus subtilis, Bacillus velez, Bacillus megaterium, Bacillus mucilaginosus, and Bacillus laterosporus. Thus, it can be adjusted according to demand to improve various properties of crops.

[0125] According to an embodiment of the present application, the number of live bacteria in the bacterial solution is 1 billion / mL-30 billion / mL.

[0126] According to an embodiment of the present application, the “microorganism-crop” interaction signal substance includes acetoin and 2,3-butanediol.

[0127] In this article, without special explanation, the "microorganism-crop" interaction signal substance can be a microbial fermentation product obtained by microbial fermentation, and the microbial fermentation product contains acetoin and 2,3-butanediol; it can also be obtained by preparing acetoin and 2,3-butanediol. The specific type of the "microorganism-crop" interaction signal substance is not limited, as long as it contains acetoin and 2,3-butanediol, it is within the scope of protection of this application.

[0128] According to an embodiment of the present application, the acetoin is selected from D-(-)-acetoin and / or L-(+)-acetoin, preferably D-(-)-acetoin and L-(+)-acetoin.

[0129] According to an embodiment of the present application, in the "microorganism-crop" interaction signal substance, the mass ratio of acetoin and 2,3-butanediol is (30-60): (10-30), for example (30-50): (10-30), (30-40): (10-30), (40-60): (10-30), (40-50): (10-30), (50-60): (10-30), (30-50): (10~20), (30~40): (10~20), (40~60): (10~20), (40~50): (10~20), (50~60): (10~20), (30~50): (20~30), (30~40): (20~30), (40~60): (20~30), (40~50): (20~30), (50~60): (20~30).

[0130] According to an embodiment of the present application, in the "microorganism-crop" interaction signal substance, the concentration of acetoin is 30 g / L to 60 g / L, for example, 30 g / L, 31 g / L, 32 g / L, 33 g / L, 34 g / L, 35 g / L, 36 g / L, 37 g / L, 38 g / L, 39 g / L, 40 g / L, 41 g / L, 42 g / L, 43 g / L, 44 g / L, 45 g / L, 46 g / L, 47 g / L, 48 g / L, 49 g / L, 50 g / L, 51 g / L, 52 g / L, 53 g / L, 54 g / L, 55 g / L, 56 g / L, 57 g / L, 58 g / L, 59 g / L, 60 g / L or a range value between any two of these values ​​as endpoint values.

[0131] According to an embodiment of the present application, in the "microorganism-crop" interaction signal substance, the concentration of the 2,3-butanediol is 10 g / L to 30 g / L, for example, 10 g / L, 11 g / L, 12 g / L, 13 g / L, 14 g / L, 15 g / L, 16 g / L, 17 g / L, 18 g / L, 19 g / L, 20 g / L, 21 g / L, 22 g / L, 23 g / L, 24 g / L, 25 g / L, 26 g / L, 27 g / L, 28 g / L, 29 g / L, 30 g / L or a range value between any two of these values ​​as endpoint values.

[0132] According to an embodiment of the present application, the amino acid is selected from a single amino acid or a compound amino acid.

[0133] According to the embodiment of the present application, the mass percentage of free amino acids in the composite amino acid is 20% to 30%. Amino acids can promote the growth and development of crops. Amino acids can provide a direct source of nutrition for crops, stimulate and promote crops to germinate and root faster, strengthen seedlings and trees, promote flowers and fruits, thereby increasing the overall growth rate and yield of crops; they can also complex and stabilize fertilizer nutrients, promote stable supply of fertilizer nutrients, reduce nutrient waste, and improve nutrient absorption efficiency of crops; they can also activate the synthesis of crop active enzymes, improve the metabolic capacity and stress resistance of crops, such as drought resistance and disease resistance, so that crops can maintain a good growth state even in adverse environments; they can also promote increased production and income. Compared with other fertilizers, amino acid fertilizers are more effective in increasing crop yields and improving crop quality, bringing higher economic benefits to farmers.

[0134] According to the embodiments of the present application, the seaweed essence contains alginic acid. Alginic acid can promote the growth and development of seedlings. Alginic acid can increase the hormone content in plants, promote the growth and development of seedlings, and enable crops to obtain a good growth foundation in the early stages of growth; it can also enhance plant immunity, by improving the disease resistance of crops, reducing the occurrence of diseases, and ensuring the healthy growth of crops; it can also improve soil quality. Alginic acid can increase the number and activity of microorganisms in the soil, thereby improving soil fertility and permeability, and providing a better growth environment for crops; it can also improve crop yield and quality. By promoting crop growth and development and photosynthesis efficiency, alginic acid can increase crop yield and improve the quality of agricultural products.

[0135] According to an embodiment of the present application, the mass percentage of alginate in the seaweed essence is 20% to 40%, for example, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, 40% or a range value between any two of these values ​​as endpoint values.

[0136] According to the embodiments of the present application, the humic acid contains fulvic acid. Humic acid can improve the soil environment. Humic acid has colloidal properties, can absorb and complex harmful substances in the soil, improve the natural purification ability of the soil, and reduce pollution; it can also improve the microbial population in the soil and promote the growth and reproduction of beneficial bacteria; it can also stimulate plant growth. Humic acid contains a variety of active groups, which can stimulate plant physiological metabolism, promote early germination of seeds, high germination rate, accelerate nutrient circulation and absorption, make crops grow stronger, and photosynthesis stronger; it can also improve fertilizer efficiency. Humic acid has strong exchange and adsorption capacity, can reduce the loss of fertilizer nutrients, improve nitrogen fertilizer utilization rate, and make fertilizer effect better; it can also further improve the efficacy of pesticides and protect the environment. Humic acid has a good inhibitory effect on certain plant pathogens, can improve the efficacy of pesticides, reduce pesticide damage, and protect the environment at the same time.

[0137] According to an embodiment of the present application, the mass percentage of fulvic acid in the humic acid is 20% to 40%, for example, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, 40% or a range value between any two of the endpoint values.

[0138] According to an embodiment of the present application, the mass ratio of at least one of the amino acid, alginic acid, and humic acid, the bacterial liquid, and the "microorganism-crop" interaction signal substance is (1-10): (30-70): (5-20), for example (1-6): (30-70): (5-20), (1-10): (30-70): (10-20), (1-6): (30-70): (10-20), (1-4): (30-70): (10-20), (4-6): (30-70): (10-20).

[0139] According to an embodiment of the present application, the liquid bacterial agent is selected from any one of the following groups:

[0140] 1) The liquid bacterial agent includes bacterial liquid, "microorganism-crop" interaction signal substance, and seaweed essence,

[0141] Wherein, the microorganism in the bacterial liquid is Bacillus Velezii,

[0142] The "microorganism-crop" interaction signal substances include acetoin and 2,3-butanediol;

[0143] 2) The liquid bacterial agent includes bacterial liquid, "microorganism-crop" interaction signal substance, seaweed essence, and compound amino acids,

[0144] Wherein, the microorganism in the bacterial liquid is Bacillus subtilis,

[0145] The "microorganism-crop" interaction signal substances include acetoin and 2,3-butanediol;

[0146] 3) The liquid bacterial agent includes bacterial liquid, "microorganism-crop" interaction signal substance, and humic acid,

[0147] Wherein, the microorganism in the bacterial liquid is Bacillus megaterium,

[0148] The "microorganism-crop" interaction signal substances include acetoin and 2,3-butanediol;

[0149] 4) The liquid bacterial agent includes bacterial liquid, "microorganism-crop" interaction signal substance, seaweed essence, and compound amino acids,

[0150] Wherein, the microorganisms in the bacterial liquid are Bacillus subtilis and Bacillus megaterium,

[0151] The "microorganism-crop" interaction signal substances include acetoin and 2,3-butanediol;

[0152] 5) The liquid bacterial agent includes bacterial liquid, "microorganism-crop" interaction signal substance, and seaweed essence,

[0153] Wherein, the microorganisms in the bacterial liquid are Bacillus Velez and Bacillus mucilaginosus,

[0154] The "microorganism-crop" interaction signal substances include acetoin and 2,3-butanediol;

[0155] 6) The liquid bacterial agent includes bacterial liquid, "microorganism-crop" interaction signal substance, and humic acid,

[0156] Wherein, the microorganisms in the bacterial solution are Bacillus subtilis, Bacillus megaterium and Bacillus mucilaginosus,

[0157] The "microorganism-crop" interaction signal substances include acetoin and 2,3-butanediol;

[0158] 7) The liquid bacterial agent includes bacterial liquid, "microorganism-crop" interaction signal substance, and seaweed essence,

[0159] Wherein, the microorganisms in the bacterial solution are Bacillus Velez, Bacillus mucilaginosus and Bacillus laterosporus,

[0160] The “microorganism-crop” interaction signal substances include acetoin and 2,3-butanediol.

[0161] According to an embodiment of the present application, the liquid bacterial agent is a bacterial agent.

[0162] According to an embodiment of the present application, the liquid bacterial agent is a liquid bacterial agent.

[0163] According to an embodiment of the present application, the pH of the liquid bacterial agent is 5.0-8.0, for example, 5.0, 5.5, 6.0, 6.5, 7.0, 7.5, 8.0 or a range value between any two of these values ​​as endpoint values.

[0164] Liquid inoculant

[0165] In a third aspect of the present application, the present application proposes a liquid bacterial agent. According to an embodiment of the present application, the liquid bacterial agent is selected from any one of the following groups:

[0166] 1) The liquid bacterial agent comprises bacterial liquid, "microorganism-crop" interaction signal substance and seaweed essence, and the weight ratio of the bacterial liquid, "microorganism-crop" interaction signal substance and seaweed essence is (30-70): (10-20): (1-4);

[0167] The microorganism in the bacterial solution is Bacillus Velez, and the number of viable bacteria of Bacillus Velez in the bacterial solution is 15-30 billion / mL.

[0168] The "microorganism-crop" interaction signal substance includes acetoin and 2,3-butanediol. In the "microorganism-crop" interaction signal substance, the concentration of acetoin is 30 g / L to 60 g / L, and the concentration of 2,3-butanediol is 10 g / L to 30 g / L.

[0169] The mass percentage of alginic acid in the seaweed essence is 20% to 40%;

[0170] 2) The liquid bacterial agent comprises bacterial liquid, "microorganism-crop" interaction signal substance, seaweed essence and composite amino acid, and the weight ratio of the bacterial liquid, "microorganism-crop" interaction signal substance, seaweed essence and composite amino acid is (30-70): (10-20): (0.5-2): (0.5-2);

[0171] The microorganism in the bacterial solution is Bacillus subtilis, and the number of viable bacteria of Bacillus subtilis in the bacterial solution is 15-25 billion / mL.

[0172] The "microorganism-crop" interaction signal substance includes acetoin and 2,3-butanediol. In the "microorganism-crop" interaction signal substance, the concentration of acetoin is 30 g / L to 60 g / L, and the concentration of 2,3-butanediol is 10 g / L to 30 g / L.

[0173] The mass percentage of alginic acid in the seaweed essence is 20% to 40%.

[0174] The mass percentage of free amino acids in the composite amino acids is 20% to 30%;

[0175] 3) The liquid bacterial agent comprises bacterial liquid, "microorganism-crop" interaction signal substance and humic acid, and the weight ratio of the bacterial liquid, "microorganism-crop" interaction signal substance and humic acid is (30-70): (10-20): (4-6);

[0176] The microorganism in the bacterial solution is Bacillus megaterium, and the number of viable bacteria of Bacillus megaterium in the bacterial solution is 5-10 billion / mL.

[0177] The "microorganism-crop" interaction signal substance includes acetoin and 2,3-butanediol. In the "microorganism-crop" interaction signal substance, the concentration of acetoin is 30 g / L to 60 g / L, and the concentration of 2,3-butanediol is 10 g / L to 30 g / L.

[0178] The mass percentage of fulvic acid in the humic acid is 20% to 40%;

[0179] 4) The liquid bacterial agent comprises bacterial liquid, "microorganism-crop" interaction signal substance, seaweed essence and composite amino acid, and the weight ratio of the bacterial liquid, "microorganism-crop" interaction signal substance, seaweed essence and composite amino acid is (30-70), (10-20), (2-3), (2-3);

[0180] The microorganisms in the bacterial solution are Bacillus subtilis and Bacillus megaterium, the number of live bacteria of Bacillus subtilis in the bacterial solution is 10-20 billion / mL, and the number of live bacteria of Bacillus megaterium in the bacterial solution is 100-100 million / mL.

[0181] The "microorganism-crop" interaction signal substance includes acetoin and 2,3-butanediol. In the "microorganism-crop" interaction signal substance, the concentration of acetoin is 30 g / L to 60 g / L, and the concentration of 2,3-butanediol is 10 g / L to 30 g / L.

[0182] The mass percentage of alginic acid in the seaweed essence is 20% to 40%.

[0183] The mass percentage of free amino acids in the composite amino acids is 20% to 30%;

[0184] 5) The liquid bacterial agent comprises bacterial liquid, "microorganism-crop" interaction signal substance and seaweed essence, and the weight ratio of the bacterial liquid, "microorganism-crop" interaction signal substance and seaweed essence is (30-70): (10-20): (1-2);

[0185] The microorganisms in the bacterial solution are Bacillus Velez and Bacillus mucilaginosus, the number of live bacteria of Bacillus Velez in the bacterial solution is 10-20 billion / mL, and the number of live bacteria of Bacillus mucilaginosus in the bacterial solution is 5-2 billion / mL.

[0186] The "microorganism-crop" interaction signal substance includes acetoin and 2,3-butanediol. In the "microorganism-crop" interaction signal substance, the concentration of acetoin is 30 g / L to 60 g / L, and the concentration of 2,3-butanediol is 10 g / L to 30 g / L.

[0187] The mass percentage of alginic acid in the seaweed essence is 20% to 40%;

[0188] 6) The liquid bacterial agent comprises bacterial liquid, "microorganism-crop" interaction signal substance and humic acid, and the weight ratio of the bacterial liquid, "microorganism-crop" interaction signal substance and humic acid is (30-70): (10-20): (4-6);

[0189] The microorganisms in the bacterial solution are Bacillus subtilis, Bacillus megaterium and Bacillus colloids. The number of live bacteria of Bacillus subtilis in the bacterial solution is 5-10 billion / mL, the number of live bacteria of Bacillus megaterium in the bacterial solution is 5-2 billion / mL, and the number of live bacteria of Bacillus colloids in the bacterial solution is 1-1 billion / mL.

[0190] The "microorganism-crop" interaction signal substance includes acetoin and 2,3-butanediol. In the "microorganism-crop" interaction signal substance, the concentration of acetoin is 30 g / L to 60 g / L, and the concentration of 2,3-butanediol is 10 g / L to 30 g / L.

[0191] The mass percentage of fulvic acid in the humic acid is 20% to 40%;

[0192] 7) The liquid bacterial agent comprises bacterial liquid, "microorganism-crop" interaction signal substance and seaweed essence, and the weight ratio of the bacterial liquid, "microorganism-crop" interaction signal substance and seaweed essence is (30-70): (10-20): (1-2);

[0193] The microorganisms in the bacterial solution are Bacillus Velez, Bacillus mucilaginosus and Bacillus laterosporus. The number of live bacteria of Bacillus Velez in the bacterial solution is 5-20 billion / mL, the number of live bacteria of Bacillus mucilaginosus in the bacterial solution is 100-500 million / mL, and the number of live bacteria of Bacillus laterosporus in the bacterial solution is 10-20 billion / mL.

[0194] The "microorganism-crop" interaction signal substance includes acetoin and 2,3-butanediol. In the "microorganism-crop" interaction signal substance, the concentration of acetoin is 30 g / L to 60 g / L, and the concentration of 2,3-butanediol is 10 g / L to 30 g / L.

[0195] The mass percentage of alginic acid in the seaweed essence is 20% to 40%.

[0196] The microbial strains in the liquid microbial agent of the present application can decompose organic matter in the soil, release the nutrients required by crops, and secrete some growth-promoting functional substances to promote the development of crop roots and enhance the crop's ability to absorb nutrients, thereby accelerating the growth rate of crops and improving the growth quality of crops, thereby significantly increasing crop yields; at the same time, the microbial strains with disease-resistant functions can compete with pathogens for nutrients and space, inhibit the growth and reproduction of pathogens, and they can also secrete some substances with antibacterial effects to directly kill or inhibit the activity of pathogens, thereby effectively preventing and controlling the occurrence of crop diseases. Moreover, through the synergistic effect of natural substances such as amino acids, seaweed essence, humic acid, "microorganism-crop" interaction signal substances, and microbial agents, the activity of soil microorganisms can be significantly improved, by increasing the number and type of beneficial microorganisms in the soil, improving the abundance of indigenous beneficial bacteria in the soil, improving the soil microecological environment, so that the alkaline phosphatase activity and microbial respiration intensity in the soil are significantly enhanced, which can not only improve the fertility of the soil, promote the absorption and utilization of nutrients by crops, but also enhance the ecological function of the soil and maintain the ecological balance of the soil; it also has the function of promoting crop growth and increasing crop yield; and it has the function of preventing and controlling crop diseases, and it can also improve the activity of soil microorganisms, so that the alkaline phosphatase activity and microbial respiration intensity of the soil are significantly enhanced. Therefore, the liquid microbial agent of the present application can effectively promote crop growth and the effective regulation of soil microecology, and provides strong technical support for the sustainable development of modern agriculture.

[0197] In a fourth aspect of the present application, the present application proposes a liquid bacterial agent. According to an embodiment of the present application, the liquid bacterial agent is selected from any one of the following groups:

[0198] 1) The liquid bacterial agent comprises bacterial liquid, "microorganism-crop" interaction signal substance and seaweed essence, and the weight ratio of the bacterial liquid, "microorganism-crop" interaction signal substance and seaweed essence is (55-65): (10-20): 4;

[0199] The microorganism in the bacterial solution is Bacillus Velez, and the number of viable bacteria of Bacillus Velez in the bacterial solution is 20-30 billion / mL.

[0200] The "microorganism-crop" interaction signal substance includes acetoin and 2,3-butanediol. In the "microorganism-crop" interaction signal substance, the concentration of acetoin is 40g / L to 60g / L, and the concentration of 2,3-butanediol is 10g / L to 30g / L.

[0201] The mass percentage of alginic acid in the seaweed essence is 20% to 40%;

[0202] 2) The liquid bacterial agent comprises bacterial liquid, "microorganism-crop" interaction signal substance, seaweed essence and composite amino acid, and the weight ratio of the bacterial liquid, "microorganism-crop" interaction signal substance, seaweed essence and composite amino acid is (60-70): (10-20): 0.5: 0.5;

[0203] The microorganism in the bacterial solution is Bacillus subtilis, and the number of viable bacteria of Bacillus subtilis in the bacterial solution is 15-25 billion / mL.

[0204] The "microorganism-crop" interaction signal substance includes acetoin and 2,3-butanediol. In the "microorganism-crop" interaction signal substance, the concentration of acetoin is 30g / L to 50g / L, and the concentration of 2,3-butanediol is 10g / L to 30g / L.

[0205] The mass percentage of alginic acid in the seaweed essence is 20% to 40%.

[0206] The mass percentage of free amino acids in the composite amino acids is 20% to 30%;

[0207] 3) The liquid bacterial agent comprises bacterial liquid, "microorganism-crop" interaction signal substance and humic acid, and the weight ratio of the bacterial liquid, "microorganism-crop" interaction signal substance and humic acid is (30-40): (10-20): 4;

[0208] The microorganism in the bacterial solution is Bacillus megaterium, and the number of viable bacteria of Bacillus megaterium in the bacterial solution is 5-10 billion / mL.

[0209] The "microorganism-crop" interaction signal substance includes acetoin and 2,3-butanediol. In the "microorganism-crop" interaction signal substance, the concentration of acetoin is 40g / L to 60g / L, and the concentration of 2,3-butanediol is 10g / L to 30g / L.

[0210] The mass percentage of fulvic acid in the humic acid is 20% to 40%;

[0211] 4) The liquid bacterial agent comprises bacterial liquid, "microorganism-crop" interaction signal substance, seaweed essence and composite amino acid, and the weight ratio of the bacterial liquid, "microorganism-crop" interaction signal substance, seaweed essence and composite amino acid is (45-55), (10-20), 2.5, 2.5;

[0212] The microorganisms in the bacterial solution are Bacillus subtilis and Bacillus megaterium, the number of live bacteria of Bacillus subtilis in the bacterial solution is 10-20 billion / mL, and the number of live bacteria of Bacillus megaterium in the bacterial solution is 100-100 million / mL.

[0213] The "microorganism-crop" interaction signal substance includes acetoin and 2,3-butanediol. In the "microorganism-crop" interaction signal substance, the concentration of acetoin is 30g / L to 50g / L, and the concentration of 2,3-butanediol is 10g / L to 20g / L.

[0214] The mass percentage of alginic acid in the seaweed essence is 20% to 40%.

[0215] The mass percentage of free amino acids in the composite amino acids is 20% to 30%;

[0216] 5) The liquid bacterial agent comprises bacterial liquid, "microorganism-crop" interaction signal substance and seaweed essence, and the weight ratio of the bacterial liquid, "microorganism-crop" interaction signal substance and seaweed essence is (60-70): (10-20): 1;

[0217] The microorganisms in the bacterial solution are Bacillus Velez and Bacillus mucilaginosus, the number of live bacteria of Bacillus Velez in the bacterial solution is 10-20 billion / mL, and the number of live bacteria of Bacillus mucilaginosus in the bacterial solution is 500-1500 million / mL.

[0218] The "microorganism-crop" interaction signal substance includes acetoin and 2,3-butanediol. In the "microorganism-crop" interaction signal substance, the concentration of acetoin is 30g / L to 40g / L, and the concentration of 2,3-butanediol is 20g / L to 30g / L.

[0219] The mass percentage of alginic acid in the seaweed essence is 20% to 40%;

[0220] 6) The liquid bacterial agent comprises bacterial liquid, "microorganism-crop" interaction signal substance and humic acid: the weight ratio of the bacterial liquid, "microorganism-crop" interaction signal substance and humic acid is (40-60): (10-20): 6;

[0221] The microorganisms in the bacterial solution are Bacillus subtilis, Bacillus megaterium and Bacillus colloids, the number of live bacteria of Bacillus subtilis in the bacterial solution is 5-10 billion / mL, the number of live bacteria of Bacillus megaterium in the bacterial solution is 500-1500 million / mL, and the number of live bacteria of Bacillus colloids in the bacterial solution is 100-500 million / mL.

[0222] The "microorganism-crop" interaction signal substance includes acetoin and 2,3-butanediol. In the "microorganism-crop" interaction signal substance, the concentration of acetoin is 50g / L to 60g / L, and the concentration of 2,3-butanediol is 20g / L to 30g / L.

[0223] The mass percentage of fulvic acid in the humic acid is 20% to 40%;

[0224] 7) The liquid bacterial agent comprises bacterial liquid, "microorganism-crop" interaction signal substance and seaweed essence, and the weight ratio of the bacterial liquid, "microorganism-crop" interaction signal substance and seaweed essence is (30-40): (10-20): 1;

[0225] The microorganisms in the bacterial solution are Bacillus Velez, Bacillus mucilaginosus and Bacillus laterosporus. The number of live bacteria of Bacillus Velez in the bacterial solution is 5-15 billion / mL, the number of live bacteria of Bacillus mucilaginosus in the bacterial solution is 100-500 million / mL, and the number of live bacteria of Bacillus laterosporus in the bacterial solution is 100-150 million / mL.

[0226] The "microorganism-crop" interaction signal substance includes acetoin and 2,3-butanediol. In the "microorganism-crop" interaction signal substance, the concentration of acetoin is 30g / L to 40g / L, and the concentration of 2,3-butanediol is 10g / L to 20g / L.

[0227] The mass percentage of alginic acid in the seaweed essence is 20% to 40%.

[0228] As can be seen from the foregoing, the liquid microbial agent of the present application can effectively promote crop growth and effectively regulate soil microecology, providing strong technical support for the sustainable development of modern agriculture.

[0229] method

[0230] In the fifth aspect of the present application, the present application proposes a method for regulating crop performance. According to an embodiment of the present application, the method comprises: contacting the crop with a "microorganism-crop" interaction signal substance, the liquid microbial agent described in the second aspect, or the liquid microbial agent described in the third aspect or the fourth aspect; wherein regulating crop performance comprises at least one of the following: regulating the metabolic pathway in the crop, increasing the content of sucrose, galactose, melibiose, dehydroascorbic acid, tryptophan, gibberellin, jasmonic acid, methyl jasmonate, and ferulic acid in the crop, improving the growth and stress resistance of the crop, promoting the growth of the crop, increasing the yield of the crop, and preventing and controlling crop diseases.

[0231] In the sixth aspect of the present application, the present application proposes a method for regulating soil properties. According to an embodiment of the present application, the method comprises: contacting the soil with a "microorganism-crop" interaction signal substance, the liquid bacterial agent described in the second aspect, or the liquid bacterial agent described in the third aspect or the fourth aspect; wherein regulating soil properties comprises at least one of the following: regulating the microbial community in the soil, increasing the abundance of soil phosphate-solubilizing bacteria, increasing the activity of soil alkaline phosphatase, and increasing the respiration intensity of microorganisms in the soil.

[0232] In the seventh aspect of the present application, the present application proposes a method for preparing a small molecule "microorganism-crop" signal substance, which comprises: fermenting Bacillus subtilis LY-B01 to obtain a fermentation broth containing a small molecule "microorganism-crop" signal substance, wherein the concentration of acetoin in the fermentation broth is 30 to 60 g / L, and the concentration of 2,3-butanediol is 10 to 30 g / L.

[0233] In an optional embodiment of the present application, the fermentation temperature of the fermentation treatment is 35°C-37°C.

[0234] In an optional embodiment of the present application, the fermentation pH value of the fermentation treatment is 6.0-7.0.

[0235] In an optional embodiment of the present application, the culture time of the fermentation treatment is 48h-72h.

[0236] In an optional embodiment of the present application, the fermentation treatment is carried out in a fermentation tank, the tank pressure in the fermentation tank is 0.05KPa~0.08KPa, the dissolved oxygen content in the fermentation tank is 20%~25%, and the rotation speed in the fermentation tank is 180r / min~220r / min.

[0237] In an optional embodiment of the present application, the fermentation medium of the fermentation treatment includes the following components: 8% maltose, 2% yeast powder, 1.5% soy peptone, 0.5% sodium chloride, 0.4% potassium dihydrogen phosphate, 0.02% manganese sulfate, 0.02% zinc sulfate, 0.2% defoaming agent, and the balance is water.

[0238] In an optional embodiment of the present application, before the fermentation process, Bacillus subtilis LY-B01 seed liquid is prepared in advance.

[0239] In the eighth aspect of the present application, the present application proposes a method for preparing a small molecule "microorganism-crop" signal substance, which comprises: dissolving acetoin and 2,3-butanediol in water to obtain a solution containing the small molecule "microorganism-crop" signal substance; wherein the concentration of acetoin in the solution is 30 to 60 g / L, and the concentration of 2,3-butanediol is 10 to 30 g / L.

[0240] The scheme of the present application will be explained below in conjunction with the embodiments. It will be appreciated by those skilled in the art that the following embodiments are only used to illustrate the present application and should not be considered as limiting the scope of the present application. If no specific technology or conditions are indicated in the embodiments, the technology or conditions described in the literature in this area or the product specification are carried out. The reagents used or the instruments that do not indicate the manufacturer are all conventional products that can be obtained commercially.

[0241] The preparation method of the fermentation liquid of the small molecule "microorganism-crop" signal substance in the embodiment of the present application is as follows:

[0242] 1) Seed solution preparation: A strain of Bacillus subtilis LY-B01 was preserved in Sinochem Agriculture (Linyi) Research and Development Center Co., Ltd. The Bacillus subtilis LY-B01 preserved in the plate was inoculated into a 250 mL flask containing 100 ml of LB culture medium using an inoculation loop and cultured in a constant temperature incubator for 6 h at a temperature of 35° C. and a rotation speed of 180-220 rpm until the logarithmic growth phase.

[0243] 2) Fermentation medium was prepared according to the following formula (the following are all mass percentages): 8-15% maltose, 2% yeast powder, 1.5% soy peptone, 0.5% sodium chloride, 0.4% potassium dihydrogen phosphate, 0.02% manganese sulfate, 0.02% zinc sulfate, 0.2% defoamer, and the balance was water. The pH was adjusted to 7.0. Under 0.1 MPa, the medium was sterilized at 121° C. for 40-60 min together with the fermentation tank, and then cooled to normal pressure and temperature under reduced pressure for use.

[0244] 3) Liquid fermentation: Add the fermentation medium in step 2) into a pilot fermentation tank (15L-50L-500L), and inoculate the Bacillus subtilis LY-B01 seed liquid prepared in step 1) into the pilot fermentation tank (15L-50L-500L), maintain the tank pressure at 0.03KPa-0.08KPa at 35-37°C, ensure the dissolved oxygen content at 10%-25%, rotate at 180r / min, pH value at 6.0-7.0, culture for 48h-72h, and obtain a fermentation liquid containing small molecule "microorganism-crop" signal substances (hereinafter referred to as fermentation liquid) after the fermentation is completed.

[0245] 4) The acetoin content in the fermentation broth is 30-60 g / L, and the 2,3-butanediol content is 10-30 g / L.

[0246] Furthermore, the content and ratio of acetoin and 2,3-butanediol can be adjusted by controlling the maltose content and dissolved oxygen in the fermentation medium. The overall content of acetoin and 2,3-butanediol can be increased by increasing the maltose content in the medium, and the ratio of acetoin to 2,3-butanediol can be adjusted by adjusting the dissolved oxygen. Under high dissolved oxygen conditions, fermentation tends to produce acetoin, and under low dissolved oxygen conditions, fermentation tends to produce 2,3-butanediol. The rules obtained based on long-term production are as follows:

[0247] When the maltose content of the fermentation medium is 8%, the total content of acetoin and 2,3-butanediol is 40g / L. At this time, the dissolved oxygen in the fermentation needs to reach 25%. The final acetoin content of the fermentation liquid is 30g / L and the 2,3-butanediol content is 10g / L.

[0248] When the maltose content of the fermentation medium is 10%, the total content of acetoin and 2,3-butanediol is 50g / L. At this time, the dissolved oxygen in the fermentation needs to reach 25%. The final acetoin content of the fermentation liquid is 40g / L and the 2,3-butanediol content is 10g / L.

[0249] When the maltose content of the fermentation medium is 12%, the total content of acetoin and 2,3-butanediol is 60g / L. When the dissolved oxygen is 25%, the acetoin content in the fermentation broth is 50g / L and the 2,3-butanediol content is 10g / L; when the dissolved oxygen is 10%, the acetoin content in the fermentation broth is 30g / L and the 2,3-butanediol content is 30g / L; when the dissolved oxygen is 20%, the acetoin content in the fermentation broth is 40g / L and the 2,3-butanediol content is 20g / L.

[0250] When the maltose content of the fermentation medium is 13%, the total content of acetoin and 2,3-butanediol is 70g / L. When the dissolved oxygen is 22%, the acetoin content in the fermentation broth is 50g / L and the 2,3-butanediol content is 20g / L. When the maltose content of the fermentation medium is 15%, the total content of acetoin and 2,3-butanediol is 90g / L. When the dissolved oxygen is 18%, the acetoin content in the fermentation broth is 60g / L and the 2,3-butanediol content is 30g / L.

[0251] Example 1: Regulation of soil microbial communities by small molecule “microbe-crop” interaction signal substances

[0252] The rapeseed pot experiment was used to test the regulatory effect of "microbe-crop" interaction signal substances on soil microbial communities. Three samples of "microbe-crop" interaction signal substances were included:

[0253] Sample 1: The acetoin content is 60 g / L and the 2,3-butanediol content is 30 g / L.

[0254] Sample 2: The acetoin content is 40 g / L, and the 2,3-butanediol content is 20 g / L.

[0255] Sample 3: The acetoin content is 30 g / L, and the 2,3-butanediol content is 10 g / L.

[0256] Each pot was filled with 2 kg of soil. After rapeseed seedlings emerged, the seedlings were thinned out, leaving 3 seedlings in each pot. Samples 1, 2, and 3 containing small molecule "microorganism-crop" interaction signal substances were applied 10 days and 20 days after rapeseed seedlings emerged. The application amount was 1000 times diluted sample, and the application amount per pot was 500 mL. The control was flushed with the same amount of water. After the rapeseed grew for 45 days, it was harvested and the metagenomic analysis of the rapeseed rhizosphere soil was carried out. For details, see Figures 1 to 10 .

[0257] Among them, the analysis of the microbial community in the rhizosphere soil of rapeseed is as follows: Figure 1 As shown, it can be seen that the samples 1, 2 and 3 containing small molecule "microorganism-crop" signal substances can increase the relative abundance of species such as Flavobacterium, Polaromonans, Lysobacter, Pseudomonas, Aminobacter, Sphingopyxis, Ensifer and Sinorhizobium in the rhizosphere soil compared with the control.

[0258] Further analysis of the metagenomic data revealed that:

[0259] Compared with the control, samples 1-3 promoted the increase in the relative abundance of genes nirK, nosZ, narH / nxrB and napA involved in denitrification regulation in soil microorganisms (see Figure 2 ).

[0260] Compared with the control, samples 1-3 promoted the increase in the relative abundance of genes gltB, gdhA and glnA / GLUL involved in organic nitrogen metabolism in soil microorganisms (see Figure 3 ).

[0261] Compared with the control, samples 1-3 significantly increased the relative abundance of nirB and nasA, the regulatory genes that promote the dissimilatory / assimilation of nitrate reduction to ammonia in soil microorganisms (see Figure 4 ).

[0262] Compared with the control, the relative abundance of the regulatory genes nrtA / cynA and nrtC / nasD, which promote nitrate assimilation / nitrogen transport in soil microorganisms, in samples 1-3 increased significantly (see Figure 5 ).

[0263] Samples 1-3 increased the relative abundance of the phosphorus stress regulatory genes phoU and phoP in soil microorganisms compared with the control (see Figure 6 ).

[0264] Compared with the control, the relative abundance of genes ugpC, pstC and pstB, which promote phosphorus absorption and transport in soil microorganisms, increased in samples 1-3 (see Figure 7 ).

[0265] Compared with the control, samples 1-3 increased the relative abundance of genes phoD, phoW, phnM and phnA related to inorganic phosphorus solubilization and organic phosphorus mineralization in soil microorganisms (see Figure 8 ).

[0266] The results of the two matrix heat maps can be found in Fig. 9, indicating that Flavobacterium, Monosporus, Lysozyme, Lactobacillus, Pseudomonas, Aminobacterium, Sphingomyelinase, Massilia, Sword, and Sinorhizobium are positively correlated with the relative abundance of NP-related genes, and are significantly positively correlated with phpP and ugpC genes (phpP genes are involved in the mineralization of soil organic phosphorus, and ugpC genes are directly involved in the transport of P). The results show that the regulation of samples 1, 2, and 3 containing small molecule "microorganism-crop" signal substances on rhizosphere microorganisms is mainly reflected in the positive regulation of soil organic phosphorus mineralization and phosphorus transport capacity.

[0267] The results of soil alkaline phosphatase activity and soil respiration intensity were determined in Fig.10 Compared with the control, the alkaline phosphatase activity of samples 1, 2 and 3 containing small molecule "microorganism-crop" interaction signal substances increased by 24.8% (sample 1), 24.40% (sample 2) and 20.16% (sample 3), and the soil respiration intensity increased by 16.84% (sample 1), 12.71% (sample 2) and 9.97% (sample 3).

[0268] Example 2: Targeted gene regulation of Arabidopsis thaliana by small molecule “microbe-crop” interaction signal substances

[0269] The model crop Arabidopsis thaliana was used to test the regulatory effects of “microbe-crop” interaction signal substances on targeted genes. Three samples of “microbe-crop” interaction signal substances were included:

[0270] Sample 1: The acetoin content is 60 g / L and the 2,3-butanediol content is 30 g / L.

[0271] Sample 2: The acetoin content is 40 g / L, and the 2,3-butanediol content is 20 g / L.

[0272] Sample 3: 30 g / L acetoin content, 10 g / L 2,3-butanediol content. When Arabidopsis thaliana had 10 leaves, samples 1, 2 and 3 containing small molecule "microorganism-crop" signal substances were applied. The application amount was 1000 times diluted sample, and the application amount per pot was 500 mL. The control was applied with the same amount of water. 8 hours after the application, the Arabidopsis leaves were quickly frozen and ground with liquid nitrogen, and the Arabidopsis related growth genes were detected by quantitative PCR. The results are as follows Fig.11 .

[0273] The results showed that the application of samples containing small molecule "microbe-crop" interaction signal substances can target and regulate Arabidopsis-related growth-promoting genes. Compared with the control, the application of samples 1, sample 2, and sample 3 containing the small molecule "microbe-crop" interaction signal substances can increase the relative abundance of N metabolism-related genes NRT1.9, NRT2.5, and NRT1.5 by 4.2, 4.2, and 4.6 times (sample 1), 4.1, 4.0, and 4.5 times (sample 2), and 3.9, 3. .8 and 4.4 times (sample 3); the relative abundance of auxin synthesis genes YUC8, YUC3, YUC2, and TAA1 were increased by 2.8, 3.0, 3.4, and 3.7 times (sample 1), 2.7, 2.9, 3.3, and 3.5 times (sample 2), and 2.6, 2.8, 3.2, and 3.4 times (sample 3), respectively; the relative abundance of photosynthetic carbon fixation gene RCA was increased by 6.9 times (sample 1), 6.5 times (sample 1), and 6.3 times (sample 3).

[0274] Example 3: Regulation of Physiological Metabolism of Dwarf Tomato by Small-molecule “Microbe-Crop” Interaction Signal Substances

[0275] In the dwarf tomato pot experiment, samples containing small molecule "microbe-crop" interaction signal substances were applied at the 15-leaf stage. The samples included three:

[0276] Sample 1: The acetoin content is 60 g / L and the 2,3-butanediol content is 30 g / L.

[0277] Sample 2: The acetoin content is 40 g / L, and the 2,3-butanediol content is 20 g / L.

[0278] Sample 3: The acetoin content is 30 g / L, and the 2,3-butanediol content is 10 g / L.

[0279] The amount of irrigation was 500 mL per pot after diluting the sample 1000 times, and the control was the same amount of water. At 3, 5, and 8 days after irrigation, leaf tissues were collected, quickly frozen and ground with liquid nitrogen. Non-targeted metabolite analysis was performed using LC-MS to determine the effects of sample 1, sample 2, and sample 3 containing small molecule "microbe-crop" interaction signal substances on the physiological metabolism of dwarf tomatoes. At 3, 5, and 8 days after irrigation, the following metabolic pathways in leaf tissues were always positively regulated.

[0280]

[0281]

[0282] The aboveground fresh weight and root fresh weight of dwarf tomatoes were measured on the 8th day after the application of samples 1, 2 and 3. The aboveground fresh weight and root fresh weight of dwarf tomatoes applied with samples 1, 2 and 3 containing small molecule "microorganism-crop" interaction signal substances were 12.96g / plant and 3.78g / plant (sample 1), 12.15g / plant and 3.62g / plant (sample 2), and 11.99g / plant and 3.57g / plant (sample 3), respectively; the aboveground fresh weight and root fresh weight of the control tomatoes were only 10.95g / plant and 3.01g / plant, respectively.

[0283] The above results show that the application of samples 1, 2 and 3 containing small molecule "microorganism-crop" interaction signal substances can increase the content of sucrose, galactose and melibiose in crops by regulating galactose metabolism; can increase the content of dehydroascorbic acid in crops by regulating ascorbic acid and aldehyde metabolism; can increase the content of tryptophan in crops by regulating tryptophan metabolism; can increase the content of gibberellins in crops by regulating diterpenoid biosynthesis; can increase the content of jasmonic acid and methyl jasmonate in crops by regulating linolenic acid metabolism; can increase the content of ferulic acid in crops by regulating phenylpropanoid biosynthesis. Through this experiment, it can be seen that samples 1, 2 and 3 containing small molecule "microorganism-crop" interaction signal substances can positively regulate the physiological metabolic pathways related to dwarf tomatoes, increase the content of dwarf tomato related metabolites, and thus promote the growth of dwarf tomatoes.

[0284] Example 4: Single-bacteria liquid inoculant product

[0285] 1. Preparation of single-bacteria liquid inoculant product 1:

[0286] The Bacillus Velezii was deposited in the General Microbiology Center of China National Microbiological Culture Collection Administration with the deposit number CGMCC No. 21827. After liquid fermentation, the number of viable bacteria in the fermentation liquid was 15 billion / mL. The pH of the fermentation liquid was adjusted to 6, and then the fermentation liquid was concentrated 1 times by a disc centrifuge, and the number of viable bacteria reached 30 billion / mL, which was marked as material A.

[0287] Seaweed extract (purchased from Qingdao Haida Biological) was used as a natural product, wherein the alginate content of the seaweed extract was 30%, and was marked as material B.

[0288] The fermentation broth containing small molecule "microorganism-crop" signal substances (the content of acetoin is 50 g / L and the content of 2,3-butanediol is 20 g / L) is marked as material C.

[0289] After fully mixing 600kg of material A and 200kg of material C, add material B into the mixture, of which 40kg is added. In a temperature-controlled reactor or fermentation tank, keep the liquid temperature at about 25°C, and stir thoroughly for ≥4h to obtain a single-bacteria liquid inoculant product 1. The viable count of this product is marked as ≥10 billion / mL, and the 12-month viable count tracking is as follows:

[0290]

[0291] A tomato pot experiment was used to test the agronomic effect of liquid microbial agent product 1. Each pot was filled with 8 kg of soil. After the tomatoes emerged, the seedlings were thinned out, leaving 1 seedling in each pot, 20 pots / treatment, and a total of 140 pots of tomatoes. Liquid microbial agent product 1 (material A: material B: material C = 600:40:200) was applied to the tomatoes 15 days and 30 days after they emerged, respectively. At the same time, equal amounts of controls A, B, C, A+B, A+C, and B+C were applied. The application amount was 1000 times diluted, and the application amount per pot was 500 mL. The tomatoes were harvested after 45 days of growth, and the tomato plant height, aboveground fresh weight, underground fresh weight, rhizosphere soil alkaline phosphatase activity, and rhizosphere soil microbial respiration intensity were measured. The results are as follows:

[0292]

[0293] 2. Preparation of single-bacteria liquid inoculant product 2:

[0294] Bacillus subtilis was deposited in the General Microbiology Center of China National Microbiological Culture Collection Administration with the deposit number CGMCC No. 21826. After liquid fermentation, the number of viable bacteria in the fermentation liquid was 20 billion / mL. The pH of the fermentation liquid was adjusted to 7.0 and marked as material A.

[0295] Seaweed extract (purchased from Qingdao Haida Biological) and compound amino acids (purchased from Hebei Hongtao Biological) were used as natural products, wherein the alginate content of the seaweed extract was 30%, the free amino acid content of the compound amino acids was 25%, and the weight ratio of the seaweed extract to the compound amino acids was 1:1. The material was marked as material B.

[0296] The fermentation broth containing small molecule "microorganism-crop" signal substances (the content of acetoin is 40 g / L and the content of 2,3-butanediol is 20 g / L) is marked as material C.

[0297] After fully mixing 700kg of material A and 100kg of material C, add material B into the mixture, of which 10kg is added. In a temperature-controlled reactor or fermentation tank, keep the liquid temperature at about 25°C, and stir it fully for ≥4h to obtain a single-bacteria liquid inoculant product 2. The viable count of this product is marked as ≥10 billion / mL. The 12-month viable count tracking is as follows:

[0298]

[0299] The agronomic effect of liquid microbial agent product 2 was tested in a tomato pot experiment. Each pot was filled with 8 kg of soil. After the tomato seedlings emerged, the seedlings were thinned out, leaving 1 seedling in each pot, 20 pots / treatment, and a total of 140 pots of tomatoes. Liquid microbial agent product 2 (material A: material B: material C = 700:10:100) was applied 15 days and 30 days after the tomato seedlings emerged, and the same amount of control A, B, C, A+B, A+C, B+C was applied at the same time. The application amount was 1000 times diluted, and the application amount per pot was 500 mL. The tomatoes were harvested after 45 days of growth, and the tomato plant height, aboveground fresh weight, underground fresh weight, rhizosphere soil alkaline phosphatase activity, and rhizosphere soil microbial respiration intensity were measured. The results are as follows:

[0300]

[0301]

[0302] 3. Preparation of single-bacteria liquid inoculant product 3:

[0303] Bacillus megaterium was deposited in the General Microbiology Center of China National Microbiological Culture Collection Administration with the deposit number CGMCC No. 21828. After liquid fermentation, the number of live bacteria in the fermentation liquid was 3.5 billion / mL. The pH of the fermentation liquid was adjusted to 7.0, and then the fermentation liquid was concentrated 1 times by a disc centrifuge, and the number of live bacteria reached 7 billion / mL, which was marked as material A.

[0304] The fermentation broth containing small molecule "microorganism-crop" signal substances (the content of acetoin is 50 g / L and the content of 2,3-butanediol is 10 g / L) is marked as material B.

[0305] Humic acid (purchased from Shanxi Jinfeng Biology) was used as a natural product, wherein the fulvic acid content in the humic acid was 30%, and was marked as material C.

[0306] After fully mixing 300kg of material A and 100kg of material B, add material C into the mixture, of which 40kg of material C is added. In a temperature-controlled reactor or fermentation tank, keep the liquid temperature at about 25°C, and stir it for ≥4h to obtain a single-bacteria liquid inoculant product 3. The viable count of this product is marked as ≥1 billion / mL. The 12-month viable count tracking is as follows:

[0307]

[0308] 4. The preparation of single-bacteria liquid inoculant product 4 refers to single-bacteria liquid inoculant product 1. The only difference is that the source of the small molecule "microorganism-crop" signal substance is different. The small molecule "microorganism-crop" signal substance is prepared by formulation (containing 50 g / L of acetoin and 20 g / L of 2,3-butanediol). The specific preparation method is: after mixing acetoin (McLean, product number A800759) and 2,3-butanediol (McLean, product number B822252) in a weight ratio of 5:2, weigh 70 g of the mixed sample, dissolve it and make up to 1 L of water, and prepare single-bacteria liquid inoculant product 4 with reference to single-bacteria liquid inoculant product 1.

[0309] Example 5: Composite liquid bacterial agent product

[0310] 1. Preparation of composite liquid bacterial agent product 1:

[0311] The Bacillus subtilis was subjected to liquid fermentation, and the number of live bacteria in the fermentation liquid was 20 billion / mL, and the pH of the fermentation liquid was adjusted to 7.0; the Bacillus megaterium was subjected to liquid fermentation, and the number of live bacteria in the fermentation liquid was 3.5 billion / mL, and the pH of the fermentation liquid was adjusted to 7.0; the Bacillus subtilis and Bacillus megaterium fermentation liquids were fully mixed in a volume ratio of 5:1, and the mixture was marked as material A.

[0312] The fermentation broth containing small molecule "microorganism-crop" signal substances (the content of acetoin is 40g / L and the content of 2,3-butanediol is 10g / L) is marked as material B.

[0313] Seaweed extract (purchased from Qingdao Haida Biological) and compound amino acids (Hebei Hongtao Biological) were used as natural products, wherein the alginate content of the seaweed extract was 30%, the free amino acid content of the compound amino acids was 25%, and the weight ratio of the seaweed extract to the compound amino acids was 1:1. The material was marked as material C.

[0314] After fully mixing 500kg of material A and 200kg of material B, add material C into the mixture, of which 50kg of material C is added. In a temperature-controlled reactor or fermentation tank, keep the liquid temperature at about 25°C, and stir it fully for ≥4h to obtain the composite liquid bacterial agent product 1. The viable count of this product is marked as ≥10 billion / mL. The 12-month viable count tracking is as follows:

[0315]

[0316] The agronomic effect of the composite liquid microbial agent product 1 was tested in a tomato pot experiment. Each pot was filled with 8 kg of soil. After the tomatoes emerged, the seedlings were thinned out, leaving one seedling in each pot, 20 pots / treatment, and a total of 140 pots of tomatoes. Composite liquid microbial agent product 1 (material A: material B: material C = 500:200:50) was applied 15 days and 30 days after the emergence of tomatoes, respectively. At the same time, equal amounts of controls A, B, C, A+B, A+C, and B+C were applied. The application amount was 1000 times diluted with the product, and the application amount per pot was 500 mL. The tomatoes were harvested after 45 days of growth, and the tomato plant height, aboveground fresh weight, underground fresh weight, rhizosphere soil alkaline phosphatase activity, and rhizosphere soil microbial respiration intensity were measured. The results are as follows:

[0317]

[0318] 2. Preparation of composite liquid bacterial agent product 2:

[0319] The Bacillus Velez subtilis was subjected to liquid fermentation, and the number of live bacteria in the fermentation liquid was 15 billion / mL. The pH of the fermentation liquid was adjusted to 7.0, and then the fermentation liquid was concentrated 1 times by a disc centrifuge, and the number of live bacteria reached 30 billion / mL; the Bacillus colloids was subjected to liquid fermentation, and the number of live bacteria in the fermentation liquid was 2 billion / mL, and the pH of the fermentation liquid was adjusted to 7.0; the Bacillus Velez subtilis and the Bacillus colloids fermentation liquid were fully mixed in a volume ratio of 1:1 and marked as material A.

[0320] The fermentation broth containing small molecule "microorganism-crop" signal substances (the content of acetoin is 30 g / L and the content of 2,3-butanediol is 30 g / L) is marked as material B.

[0321] Seaweed extract (purchased from Qingdao Haida Biological) was used as a natural product, wherein the alginate content of the seaweed extract was 30%, and was marked as material C.

[0322] After fully mixing 700kg of material A and 200kg of material B, add material C into the mixture, of which 10kg of material C is added. In a temperature-controlled reactor or fermentation tank, keep the liquid temperature at about 25°C, and stir it for ≥4h to obtain the composite liquid bacterial agent product 2. The viable count of this product is marked as ≥5 billion / mL. The 12-month viable count tracking is as follows:

[0323]

[0324] The agronomic effect of the composite liquid microbial agent product 2 was tested by a tomato pot experiment. Each pot was filled with 8 kg of soil. After the tomatoes emerged, the seedlings were thinned out, leaving 1 seedling in each pot, 20 pots / treatment, and a total of 140 pots of tomatoes. Composite liquid microbial agent product 2 (material A: material B: material C = 700:200:10) was applied 15 days and 30 days after the emergence of tomatoes, and the same amount of control A, B, C, A+B, A+C, B+C was applied at the same time. The application amount was 1000 times diluted with the product, and the application amount per pot was 500 mL. The tomatoes were harvested after 45 days of growth, and the tomato plant height, aboveground fresh weight, underground fresh weight, rhizosphere soil alkaline phosphatase activity, and rhizosphere soil microbial respiration intensity were measured. The results are as follows:

[0325]

[0326]

[0327] 3. Preparation of composite liquid bacterial agent product 3:

[0328] The Bacillus subtilis was subjected to liquid fermentation, and the number of live bacteria in the fermentation liquid was 20 billion / mL, and the pH of the fermentation liquid was adjusted to 7.0; the Bacillus megaterium was subjected to liquid fermentation, and the number of live bacteria in the fermentation liquid was 3.5 billion / mL, and the pH of the fermentation liquid was adjusted to 7.0, and then the fermentation liquid was concentrated by 1 times through a disc centrifuge, and the number of live bacteria reached 7 billion / mL; the Bacillus microglobulus was subjected to liquid fermentation, and the number of live bacteria in the fermentation liquid was 2 billion / mL, and the pH of the fermentation liquid was adjusted to 7.0; the fermentation broths of Bacillus subtilis, Bacillus megaterium, and Bacillus microglobulus were fully mixed in a volume ratio of 2:1:1 and marked as material A.

[0329] The fermentation broth containing small molecule "microorganism-crop" signal substances (acetoin content is 60g / L, 2,3-butanediol content is 30g / L) is marked as material B.

[0330] Humic acid (purchased from Shanxi Jinfeng Biology) was used as a natural product, wherein the fulvic acid content in the humic acid was 30%, and was marked as material C.

[0331] After fully mixing 500kg of material A and 100kg of material B, add material C into the mixture, of which 60kg of material C is added. In a temperature-controlled reactor or fermentation tank, keep the liquid temperature at about 25°C, and stir it for ≥4h to obtain the composite liquid bacterial agent product 3. The viable count of this product is marked as ≥5 billion / mL. The 12-month viable count tracking is as follows:

[0332]

[0333] 4. Composite liquid bacterial agent product 4:

[0334] The Bacillus Velez subtilis was subjected to liquid fermentation, and the number of live bacteria in the fermentation liquid was 15 billion / mL. The pH of the fermentation liquid was adjusted to 7.0, and then the fermentation liquid was concentrated 1 times by a disc centrifuge, and the number of live bacteria reached 30 billion / mL; the Bacillus colloids was subjected to liquid fermentation, and the number of live bacteria in the fermentation liquid was 2 billion / mL, and the pH of the fermentation liquid was adjusted to 7.0; the Bacillus laterosporus was subjected to liquid fermentation, and the number of live bacteria in the fermentation liquid was 8 billion / mL, and the pH of the fermentation liquid was adjusted to 7.0; the fermentation broths of Bacillus Velez subtilis, Bacillus colloids, and Bacillus laterosporus were fully mixed in a volume ratio of 2:1:1 and marked as material A.

[0335] The fermentation broth containing small molecule "microorganism-crop" signal substances (the content of acetoin is 30 g / L and the content of 2,3-butanediol is 10 g / L) is marked as material B.

[0336] Seaweed extract (purchased from Qingdao Haida Biological) was used as a natural product, wherein the alginate content of the seaweed extract was 30%, and was marked as material C.

[0337] After fully mixing 300kg of material A and 200kg of material B, add material C into the mixture, of which 10kg of material C is added. In a temperature-controlled reactor or fermentation tank, keep the liquid temperature at about 25°C, and stir it fully for ≥4h to obtain composite liquid bacterial agent product 4. The viable count of this product is marked as ≥5 billion / mL, and the 12-month viable count tracking is as follows:

[0338]

[0339] 5. The preparation of composite liquid inoculant product 5 refers to composite liquid inoculant product 1, with the only difference being the source of the small molecule "microorganism-crop" signal substance. The small molecule "microorganism-crop" signal substance is prepared by formulation (containing 40 g / L of acetoin and 10 g / L of 2,3-butanediol). The specific preparation method is: after mixing acetoin (McLean, Product No. A800759) and 2,3-butanediol (McLean, Product No. B822252) in a weight ratio of 4:1, weigh 50 g of the mixed sample, dissolve it and make up to 1 L of water, and prepare composite liquid inoculant product 5 with reference to composite liquid inoculant product 1.

[0340] Example 6: Liquid product effect field test

[0341] A potato field trial (Atlantic variety) was conducted in Zhenglan Banner, Northwest China, to evaluate the biocontrol and growth-promoting functions of single-bacteria liquid inoculant products 1 to 4 prepared in Example 3 and composite liquid inoculant products 1 to 5 prepared in Example 4. The trial treatment included:

[0342] serial number deal with Dosage CK none none T1 Single bacteria liquid inoculant product 1 4L / mu / time T2 Single bacteria liquid inoculant product 2 4L / mu / time T3 Single bacteria liquid inoculant product 3 10L / mu / time T4 Compound liquid bacterial agent product 1 5L / mu / time T5 Compound liquid bacterial agent product 2 8L / mu / time T6 Compound liquid bacterial agent product 3 8L / mu / time T7 Compound liquid bacterial agent product 4 8L / mu / time T8 Single bacteria liquid inoculant product 4 4L / mu / time T9 Compound liquid bacterial agent product 5 8L / mu / time

[0343] Liquid bacterial agent was applied once at potato planting (ditch application), early expansion stage (root irrigation), and late expansion stage (root irrigation). Three replicates were set for each treatment, with each replicate covering 1 mu of land. The potato yield was measured at the harvest time, and the disease index of potato scab and black mole was calculated. Among them, the grading standard of potato scab and the calculation method of prevention effect are as follows:

[0344] The grading standard for diseased potatoes (lesions / potato surface) is as follows: Grade 0: no lesions; Grade 1: lesion area <10%; Grade 2: lesion area 10%-20%; Grade 3: lesion area 20%-50%; Grade 4: lesion area >50%;

[0345] Incidence rate (%) = number of diseased plants (tubes) / total number of investigated plants (tubes) × 100;

[0346] Disease index (%) = ∑ [number of diseased plants (disease-prone tubers) at each level × representative value at each level] / [total number of plants (tubes) surveyed × representative value of the highest level] × 100;

[0347] Control effect (%) = (control disease index - treatment disease index) / control disease index × 100.

[0348] The classification standards and prevention efficiency calculation methods for potato black mole disease are as follows:

[0349] The grading standard for diseased potatoes (lesions / ulcers) is as follows: Grade 0, no sclerotia on the surface of the tuber; Grade 1, sclerotia occupy 0% to 5% of the surface area; Grade 2, sclerotia occupy 6% to 35% of the surface area; Grade 3, sclerotia occupy 36% to 65% of the surface area; Grade 4, sclerotia occupy 66% to 95% of the surface area; Grade 5, sclerotia occupy more than 96% of the surface area;

[0350] Incidence rate (%) = number of diseased plants (tubes) / total number of investigated plants (tubes) × 100;

[0351] Disease index (%) = ∑ [number of diseased plants (disease-prone tubers) at each level × representative value at each level] / [total number of plants (tubes) surveyed × representative value of the highest level] × 100;

[0352] Control effect (%) = (control disease index - treatment disease index) / control disease index × 100.

[0353] The results of the field test showed that the potato yield of T1-T9 was significantly higher than that of CK. The yield results are as follows:

[0354]

[0355]

[0356] The results of field trials showed that T1, T2, T4-T9 had good control effects on scab and mole of potato tubers. The control effects are as follows:

[0357] serial number Effect of potato scab control Effect of potato tuber black mole disease prevention and control T1 55％ 47％ T2 53％ 46％ T3 3.1％ 11％ T4 49％ 54％ T5 51％ 47％ T6 54％ 45％ T7 55％ 47％ T8 41.9％ 45.5％ T9 39.5％ 41.3％

[0358] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present application. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine the different embodiments or examples described in this specification and the features of the different embodiments or examples, without contradiction.

[0359] Although the embodiments of the present application have been shown and described above, it can be understood that the above embodiments are exemplary and cannot be understood as limitations on the present application. Ordinary technicians in this field can change, modify, replace and modify the above embodiments within the scope of the present application.

Claims

1. Use of a "microorganism-crop" interaction signal substance in preparing a product, wherein the "microorganism-crop" interaction signal substance comprises acetoin and 2,3-butanediol, and the product is used for: Modulate soil microbial communities, and / or Increase the abundance of soil phosphate-solubilizing bacteria, and / or Increase soil alkaline phosphatase activity and soil respiration, and / or Regulate metabolic pathways in crops, and / or Increase the content of sucrose, galactose, melibiose, dehydroascorbic acid, tryptophan, gibberellin, jasmonic acid, methyl jasmonate, ferulic acid in crops, and / or Improve crop growth and stress resistance, and / or Promote crop growth, and / or Increase crop yields, and / or Control crop diseases, and / or Optionally, the “microorganism-crop” interaction signal substance comprises acetoin and 2,3-butanediol.

2. A liquid bacterial agent, characterized in that: Including bacterial liquid, "microbe-crop" interaction signal substances, and At least one of amino acid, seaweed extract and humic acid.

3. The liquid bacterial agent according to claim 2, characterized in that: The bacterial liquid includes at least one of Bacillus subtilis, Bacillus velez, Bacillus megaterium, Bacillus mucilaginosus, and Bacillus laterosporus; and / or, the microorganism in the bacterial liquid is selected from Bacillus subtilis, Bacillus velez, Bacillus megaterium, Bacillus mucilaginosus, or Bacillus laterosporus; and / or, the microorganisms in the bacterial solution are selected from at least three of Bacillus subtilis, Bacillus velez, Bacillus megaterium, Bacillus mucilaginosus, and Bacillus laterosporus; And / or, the number of live bacteria in the bacterial solution is 1 billion / mL-30 billion / mL.

4. The liquid bacterial agent according to claim 2, characterized in that: The "microorganism-crop" interaction signal substances include acetoin and 2,3-butanediol; Optionally, the acetoin is selected from D-(-)-acetoin and / or L-(+)-acetoin, preferably D-(-)-acetoin and L-(+)-acetoin; And / or, in the "microorganism-crop" interaction signal substance, the mass ratio of acetoin to 2,3-butanediol is (30-60): (10-30); And / or, in the "microorganism-crop" interaction signal substance, the concentration of acetoin is 30 g / L to 60 g / L, and the concentration of 2,3-butanediol is 10 g / L to 30 g / L; and / or, the amino acid is selected from a single amino acid or a composite amino acid; And / or, the mass percentage of free amino acids in the composite amino acids is 20% to 30%; And / or, the mass percentage of alginic acid in the seaweed essence is 20% to 40%; And / or, the mass percentage of fulvic acid in the humic acid is 20% to 40%.

5. The liquid bacterial agent according to any one of claims 2 to 4, characterized in that: The mass ratio of at least one of the amino acid, alginic acid, and humic acid, the bacterial solution, and the "microorganism-crop" interaction signal substance is (1-10): (30-70): (5-20).

6. The liquid bacterial agent according to claim 2, characterized in that: The liquid bacterial agent is selected from any one of the following groups: 1) The liquid bacterial agent includes bacterial liquid, "microorganism-crop" interaction signal substance, and seaweed essence, Wherein, the microorganism in the bacterial liquid is Bacillus Velezii, The "microorganism-crop" interaction signal substances include acetoin and 2,3-butanediol; 2) The liquid bacterial agent includes bacterial liquid, "microorganism-crop" interaction signal substance, seaweed essence, and compound amino acids, Wherein, the microorganism in the bacterial liquid is Bacillus subtilis, The "microorganism-crop" interaction signal substances include acetoin and 2,3-butanediol; 3) The liquid bacterial agent includes bacterial liquid, "microorganism-crop" interaction signal substance, and humic acid, Wherein, the microorganism in the bacterial liquid is Bacillus megaterium, The "microorganism-crop" interaction signal substances include acetoin and 2,3-butanediol; 4) The liquid bacterial agent includes bacterial liquid, "microorganism-crop" interaction signal substance, seaweed essence, and compound amino acids, Wherein, the microorganisms in the bacterial liquid are Bacillus subtilis and Bacillus megaterium, The "microorganism-crop" interaction signal substances include acetoin and 2,3-butanediol; 5) The liquid bacterial agent includes bacterial liquid, "microorganism-crop" interaction signal substance, and seaweed essence, Wherein, the microorganisms in the bacterial liquid are Bacillus Velez and Bacillus mucilaginosus, The "microorganism-crop" interaction signal substances include acetoin and 2,3-butanediol; 6) The liquid bacterial agent includes bacterial liquid, "microorganism-crop" interaction signal substance, and humic acid, Wherein, the microorganisms in the bacterial solution are Bacillus subtilis, Bacillus megaterium and Bacillus mucilaginosus, The "microorganism-crop" interaction signal substances include acetoin and 2,3-butanediol; 7) The liquid bacterial agent includes bacterial liquid, "microorganism-crop" interaction signal substance, and seaweed essence, Wherein, the microorganisms in the bacterial solution are Bacillus Velez, Bacillus mucilaginosus and Bacillus laterosporus, The "microorganism-crop" interaction signal substances include acetoin and 2,3-butanediol; And / or, the pH of the liquid bacterial agent is 5.0-8.

0.

7. A liquid bacterial agent, characterized in that: Select from any of the following groups: 1) The liquid bacterial agent comprises bacterial liquid, "microorganism-crop" interaction signal substance and seaweed essence, and the weight ratio of the bacterial liquid, "microorganism-crop" interaction signal substance and seaweed essence is (30-70): (10-20): (1-4); The microorganism in the bacterial solution is Bacillus Velez, and the number of viable bacteria of Bacillus Velez in the bacterial solution is 15-30 billion / mL. The "microorganism-crop" interaction signal substance includes acetoin and 2,3-butanediol. In the "microorganism-crop" interaction signal substance, the concentration of acetoin is 30 g / L to 60 g / L, and the concentration of 2,3-butanediol is 10 g / L to 30 g / L. The mass percentage of alginic acid in the seaweed essence is 20% to 40%; 2) The liquid bacterial agent comprises bacterial liquid, "microorganism-crop" interaction signal substance, seaweed essence and composite amino acid, and the weight ratio of the bacterial liquid, "microorganism-crop" interaction signal substance, seaweed essence and composite amino acid is (30-70): (10-20): (0.5-2): (0.5-2); The microorganism in the bacterial solution is Bacillus subtilis, and the number of viable bacteria of Bacillus subtilis in the bacterial solution is 15-25 billion / mL. The "microorganism-crop" interaction signal substance includes acetoin and 2,3-butanediol. In the "microorganism-crop" interaction signal substance, the concentration of acetoin is 30 g / L to 60 g / L, and the concentration of 2,3-butanediol is 10 g / L to 30 g / L. The mass percentage of alginic acid in the seaweed essence is 20% to 40%. The mass percentage of free amino acids in the composite amino acids is 20% to 30%; 3) The liquid bacterial agent comprises bacterial liquid, "microorganism-crop" interaction signal substance and humic acid, and the weight ratio of the bacterial liquid, "microorganism-crop" interaction signal substance and humic acid is (30-70): (10-20): (4-6); The microorganism in the bacterial solution is Bacillus megaterium, and the number of viable bacteria of Bacillus megaterium in the bacterial solution is 5-10 billion / mL. The "microorganism-crop" interaction signal substance includes acetoin and 2,3-butanediol. In the "microorganism-crop" interaction signal substance, the concentration of acetoin is 30 g / L to 60 g / L, and the concentration of 2,3-butanediol is 10 g / L to 30 g / L. The mass percentage of fulvic acid in the humic acid is 20% to 40%; 4) The liquid bacterial agent comprises bacterial liquid, "microorganism-crop" interaction signal substance, seaweed essence and composite amino acid, and the weight ratio of the bacterial liquid, "microorganism-crop" interaction signal substance, seaweed essence and composite amino acid is (30-70): (10-20): (2-3): (2-3); The microorganisms in the bacterial solution are Bacillus subtilis and Bacillus megaterium, the number of live bacteria of Bacillus subtilis in the bacterial solution is 10-20 billion / mL, and the number of live bacteria of Bacillus megaterium in the bacterial solution is 100-100 million / mL. The "microorganism-crop" interaction signal substance includes acetoin and 2,3-butanediol. In the "microorganism-crop" interaction signal substance, the concentration of acetoin is 30 g / L to 60 g / L, and the concentration of 2,3-butanediol is 10 g / L to 30 g / L. The mass percentage of alginic acid in the seaweed essence is 20% to 40%. The mass percentage of free amino acids in the composite amino acids is 20% to 30%; 5) The liquid bacterial agent comprises bacterial liquid, "microorganism-crop" interaction signal substance and seaweed essence, and the weight ratio of the bacterial liquid, "microorganism-crop" interaction signal substance and seaweed essence is (30-70): (10-20): (1-2); The microorganisms in the bacterial solution are Bacillus Velez and Bacillus mucilaginosus, the number of live bacteria of Bacillus Velez in the bacterial solution is 10-20 billion / mL, and the number of live bacteria of Bacillus mucilaginosus in the bacterial solution is 5-2 billion / mL. The "microorganism-crop" interaction signal substance includes acetoin and 2,3-butanediol. In the "microorganism-crop" interaction signal substance, the concentration of acetoin is 30 g / L to 60 g / L, and the concentration of 2,3-butanediol is 10 g / L to 30 g / L. The mass percentage of alginic acid in the seaweed essence is 20% to 40%; 6) The liquid bacterial agent comprises bacterial liquid, "microorganism-crop" interaction signal substance and humic acid, and the weight ratio of the bacterial liquid, "microorganism-crop" interaction signal substance and humic acid is (30-70): (10-20): (4-6); The microorganisms in the bacterial solution are Bacillus subtilis, Bacillus megaterium and Bacillus colloids. The number of live bacteria of Bacillus subtilis in the bacterial solution is 5-10 billion / mL, the number of live bacteria of Bacillus megaterium in the bacterial solution is 5-2 billion / mL, and the number of live bacteria of Bacillus colloids in the bacterial solution is 1-1 billion / mL. The "microorganism-crop" interaction signal substance includes acetoin and 2,3-butanediol. In the "microorganism-crop" interaction signal substance, the concentration of acetoin is 30 g / L to 60 g / L, and the concentration of 2,3-butanediol is 10 g / L to 30 g / L. The mass percentage of fulvic acid in the humic acid is 20% to 40%; 7) The liquid bacterial agent comprises bacterial liquid, "microorganism-crop" interaction signal substance and seaweed essence, and the weight ratio of the bacterial liquid, "microorganism-crop" interaction signal substance and seaweed essence is (30-70): (10-20): (1-2); The microorganisms in the bacterial solution are Bacillus Velez, Bacillus mucilaginosus and Bacillus laterosporus. The number of live bacteria of Bacillus Velez in the bacterial solution is 5-20 billion / mL, the number of live bacteria of Bacillus mucilaginosus in the bacterial solution is 100-500 million / mL, and the number of live bacteria of Bacillus laterosporus in the bacterial solution is 10-20 billion / mL. The "microorganism-crop" interaction signal substance includes acetoin and 2,3-butanediol. In the "microorganism-crop" interaction signal substance, the concentration of acetoin is 30 g / L to 60 g / L, and the concentration of 2,3-butanediol is 10 g / L to 30 g / L. The mass percentage of alginic acid in the seaweed essence is 20% to 40%.

8. A liquid bacterial agent, characterized in that: Select from any of the following groups: 1) The liquid bacterial agent comprises bacterial liquid, "microorganism-crop" interaction signal substance and seaweed essence, and the weight ratio of the bacterial liquid, "microorganism-crop" interaction signal substance and seaweed essence is (55-65): (10-20): 4; The microorganism in the bacterial solution is Bacillus Velez, and the number of viable bacteria of Bacillus Velez in the bacterial solution is 20-30 billion / mL. The "microorganism-crop" interaction signal substance includes acetoin and 2,3-butanediol. In the "microorganism-crop" interaction signal substance, the concentration of acetoin is 40g / L to 60g / L, and the concentration of 2,3-butanediol is 10g / L to 30g / L. The mass percentage of alginic acid in the seaweed essence is 20% to 40%; 2) The liquid bacterial agent comprises bacterial liquid, "microorganism-crop" interaction signal substance, seaweed essence and composite amino acid, and the weight ratio of the bacterial liquid, "microorganism-crop" interaction signal substance, seaweed essence and composite amino acid is (60-70): (10-20): 0.5: 0.5; The microorganism in the bacterial solution is Bacillus subtilis, and the number of viable bacteria of Bacillus subtilis in the bacterial solution is 15-25 billion / mL. The "microorganism-crop" interaction signal substance includes acetoin and 2,3-butanediol. In the "microorganism-crop" interaction signal substance, the concentration of acetoin is 30 g / L to 50 g / L, and the concentration of 2,3-butanediol is 10 g / L to 30 g / L. The mass percentage of alginic acid in the seaweed essence is 20% to 40%. The mass percentage of free amino acids in the composite amino acids is 20% to 30%; 3) The liquid bacterial agent comprises bacterial liquid, "microorganism-crop" interaction signal substance and humic acid, and the weight ratio of the bacterial liquid, "microorganism-crop" interaction signal substance and humic acid is (30-40): (10-20): 4; The microorganism in the bacterial solution is Bacillus megaterium, and the number of viable bacteria of Bacillus megaterium in the bacterial solution is 5-10 billion / mL. The "microorganism-crop" interaction signal substance includes acetoin and 2,3-butanediol. In the "microorganism-crop" interaction signal substance, the concentration of acetoin is 40g / L to 60g / L, and the concentration of 2,3-butanediol is 10g / L to 30g / L. The mass percentage of fulvic acid in the humic acid is 20% to 40%; 4) The liquid bacterial agent comprises bacterial liquid, "microorganism-crop" interaction signal substance, seaweed essence and composite amino acid, and the weight ratio of the bacterial liquid, "microorganism-crop" interaction signal substance, seaweed essence and composite amino acid is (45-55), (10-20), 2.5, 2.5; The microorganisms in the bacterial solution are Bacillus subtilis and Bacillus megaterium, the number of live bacteria of Bacillus subtilis in the bacterial solution is 10-20 billion / mL, and the number of live bacteria of Bacillus megaterium in the bacterial solution is 100-100 million / mL. The "microorganism-crop" interaction signal substance includes acetoin and 2,3-butanediol. In the "microorganism-crop" interaction signal substance, the concentration of acetoin is 30 g / L to 50 g / L, and the concentration of 2,3-butanediol is 10 g / L to 20 g / L. The mass percentage of alginic acid in the seaweed essence is 20% to 40%. The mass percentage of free amino acids in the composite amino acids is 20% to 30%; 5) The liquid bacterial agent comprises bacterial liquid, "microorganism-crop" interaction signal substance and seaweed essence, and the weight ratio of the bacterial liquid, "microorganism-crop" interaction signal substance and seaweed essence is (60-70): (10-20): 1; The microorganisms in the bacterial solution are Bacillus Velez and Bacillus mucilaginosus, the number of live bacteria of Bacillus Velez in the bacterial solution is 10-20 billion / mL, and the number of live bacteria of Bacillus mucilaginosus in the bacterial solution is 500-1500 million / mL. The "microorganism-crop" interaction signal substance includes acetoin and 2,3-butanediol. In the "microorganism-crop" interaction signal substance, the concentration of acetoin is 30 g / L to 40 g / L, and the concentration of 2,3-butanediol is 20 g / L to 30 g / L. The mass percentage of alginic acid in the seaweed essence is 20% to 40%; 6) The liquid bacterial agent comprises bacterial liquid, "microorganism-crop" interaction signal substance and humic acid: the weight ratio of the bacterial liquid, "microorganism-crop" interaction signal substance and humic acid is (40-60): (10-20): 6; The microorganisms in the bacterial solution are Bacillus subtilis, Bacillus megaterium and Bacillus colloids, the number of live bacteria of Bacillus subtilis in the bacterial solution is 5-10 billion / mL, the number of live bacteria of Bacillus megaterium in the bacterial solution is 500-1500 million / mL, and the number of live bacteria of Bacillus colloids in the bacterial solution is 100-500 million / mL. The "microorganism-crop" interaction signal substance includes acetoin and 2,3-butanediol. In the "microorganism-crop" interaction signal substance, the concentration of acetoin is 50g / L to 60g / L, and the concentration of 2,3-butanediol is 20g / L to 30g / L. The mass percentage of fulvic acid in the humic acid is 20% to 40%; 7) The liquid bacterial agent comprises bacterial liquid, "microorganism-crop" interaction signal substance and seaweed essence, and the weight ratio of the bacterial liquid, "microorganism-crop" interaction signal substance and seaweed essence is (30-40): (10-20): 1; The microorganisms in the bacterial solution are Bacillus Velez, Bacillus mucilaginosus and Bacillus laterosporus. The number of live bacteria of Bacillus Velez in the bacterial solution is 5-15 billion / mL, the number of live bacteria of Bacillus mucilaginosus in the bacterial solution is 100-500 million / mL, and the number of live bacteria of Bacillus laterosporus in the bacterial solution is 100-150 million / mL. The "microorganism-crop" interaction signal substance includes acetoin and 2,3-butanediol. In the "microorganism-crop" interaction signal substance, the concentration of acetoin is 30 g / L to 40 g / L, and the concentration of 2,3-butanediol is 10 g / L to 20 g / L. The mass percentage of alginic acid in the seaweed essence is 20% to 40%.

9. A method for regulating crop performance, characterized in that: include: The crops are contact-treated with a "microorganism-crop" interaction signal substance, the liquid bacterial agent described in any one of claims 2 to 6, or the liquid bacterial agent described in any one of claims 7 to 8; Wherein the crop performance regulation comprises at least one of the following: Regulate the metabolic pathways in crops, Increase the content of sucrose, galactose, melibiose, dehydroascorbic acid, tryptophan, gibberellin, jasmonic acid, methyl jasmonate and ferulic acid in crops. Improve the growth and stress resistance of crops, Promote the growth of crops, Increase crop yields, Prevent and control crop diseases.

10. A method for regulating soil properties, characterized in that: include: The soil is contact treated with a "microorganism-crop" interaction signal substance, the liquid bacterial agent according to any one of claims 2 to 6, or the liquid bacterial agent according to any one of claims 7 to 8; The soil performance control comprises at least one of the following: Regulate the microbial community in the soil, Increase the abundance of soil phosphate-solubilizing bacteria, Increase soil alkaline phosphatase activity, Improve the respiration intensity of microorganisms in the soil.

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