146 results about "Flora" patented technology

The invention discloses a microorganism comprehensive index construction and intelligent prediction method and system for a water treatment process, and the method comprises the steps: obtaining microorganism samples in different types of water treatment biological systems, obtaining a relative abundance matrix through 16S rRNA high-throughput sequencing, and building a deep learning model of a mapping relation between microorganisms and abundance distribution; carrying out system disturbance simulation by utilizing a deep learning model, evaluating the influence of microbial deletion on the community structure and function, and calculating a structure key score and a function key score; the microbial basic indexes and the key microbial derivative indexes are extracted as key microbial comprehensive indexes, and a pollutant removal performance prediction model with the key microbial comprehensive indexes as input is constructed and used for predicting the pollutant removal efficiency of the complex biological treatment process. According to the method, accurate evaluation and performance prediction of the operation states of different types of complex biological treatment processes can be realized, and a scientific basis and a universal method are provided for intelligent regulation and control of flora.

The invention discloses a grouting anchor rod suitable for microorganism induced mineralization reinforcement and an application method of the grouting anchor rod. The grouting anchor rod comprises a sleeve, an anchor head, a hollow stop-grouting plug, an arch-shaped base plate and a nut, and the anchor head is detachably installed at the anchoring end of the sleeve. Through combination of the sleeve and the hollow anchor rod, grouting channels which work independently and do not interfere with each other are formed, grouting materials and biological nutrient solutions containing urease-producing flora are injected into drill holes, pore water around anchor holes and the injected nutrient solutions are used for inducing calcium carbonate to precipitate in an anchoring area, CaCO3 crystals are deposited on an anchoring interface, and the anchoring effect is achieved. Pores are filled, the bonding strength of slurry and rock soil is improved, an anchoring layer is effectively prevented from being stripped, and the interface bonding performance is enhanced; a microorganism induced mineralization technology and an anchor rod physical reinforcement system are combined, and structure-biology-environment multi-scale collaborative reinforcement is achieved; and macrostructure stability and microcosmic particle consolidation are unified, and the enhanced, lasting, stable and eco-friendly slope supporting effect is achieved.

The invention relates to the technical field of microbial cultivation, and provides an intelligent prediction method and system for spatial distribution of environmental flora, experimental group characteristics such as environmental parameters, metabolite concentration, clean room grade and the like are collected through sensors, and standardized multi-modal characteristic data are obtained through preprocessing of edge computing nodes. And training and testing the deep learning model at the cloud to obtain an intelligent prediction model. And finally, inputting real-time pre-processed data into the model, and outputting and visualizing flora structure trend data. The invention also discloses a system of the environmental flora spatial distribution intelligent prediction method, the method and the system can realize real-time acquisition, rapid preprocessing and accurate modeling of data in a culture process, fully excavate non-linear correlation of environmental parameters, experimental group characteristics and flora evolution based on a deep learning model, and improve the prediction accuracy of the spatial distribution of the environmental flora. The change trend of the flora structure can be pre-judged in advance, data support is provided for timely adjusting culture conditions and avoiding pollution risks, the operation difficulty of experimenters is reduced, and the experiment efficiency and the result repeatability are improved.

The invention discloses a bottom mud ecological restoration material as well as a preparation method and application thereof. The material is composed of a degradable carrier, a treatment flora and a multifunctional filler. The carrier is porous particles die-cast by corn straws and the like; the treatment flora comprises photosynthetic bacteria, bacillus and nitrifying bacteria; the filler is prepared from non-nitrate type hydrotalcite-like compounds, calcium nitrate, nitrate type hydrotalcite-like compounds and a flocculating agent according to a specific mass ratio. The preparation method comprises the following steps: carrying out pressure casting on the carrier, adsorbing the flora, and rolling the filler to form balls. The restoration method comprises the steps that a river channel is cut off in the dry season to form a closed area, materials are put according to the area ratio of 10: 1 for preliminary restoration, and after standing is conducted for 1-5 days, the materials are put along with water flow according to the area ratio of 2: 1 for deep restoration. Through the physical, chemical and biological synergistic effect, the sediment redox environment can be effectively improved, pollutant release and degradation are promoted, operation is easy and convenient, and the risk of secondary pollution is low.

The present disclosure relates to shaped cementitious compositions that comprise a substrate for aquatic flora and / or fauna attachment; methods for providing a substrate for aquatic flora and / or fauna attachment comprising depositing a shaped cementitious composition into a body of water; and methods for making a shaped cementitious composition. The shaped cementitious composition is formed from a pourable cementitious mixture comprising: (a) a pozzolan comprising a natural pozzolan and / or a manmade pozzolan; (b) MgO and / or Mg(OH)2; (c) an aqueous solution comprising one or more accelerants; and (d) at least one aggregate. In some cases, the MgO and / or Mg(OH)2 of (b) is included in the aqueous solution of (c). And, in various cases, the manmade pozzolan comprises a slag.

The invention relates to a flora transplantation clinical pathway standardization management method, device and equipment and a medium. The method comprises the following steps: on the basis of clinical data of a patient and screening data of a donor, executing flora suitability evaluation by using a deep learning model and selecting an adaptive donor; obtaining a flora sample of an adaptive donor, and carrying out high-throughput sequencing; generating a donor flora quality evaluation score according to a preset flora composition diversity index based on a sequencing result; screening the flora samples of which the quality evaluation scores are higher than a preset quality threshold value to prepare a flora preparation; detecting activity parameters and diversity parameters of the flora preparation, and performing colonization potential classification prediction by combining clinical data of the patient and adopting a support vector machine algorithm so as to output a potential colonization success rate; and when the potential colonization success rate exceeds a preset success rate threshold, outputting a clinical transplantation scheme through a preset transplantation scheme generation algorithm based on the flora preparation and the clinical data of the patient. According to the method, through multi-stage intelligent decision making, the safety and the effectiveness are improved.

The invention discloses a watershed ecological restoration method and a comprehensive improvement method, and relates to the technical field of watershed ecological environment treatment. The method comprises the steps that a drainage basin digital twinborn model is constructed, an ecological scheduling algorithm is embedded, and collaborative distribution of ecological water demand and production water is achieved; a climate self-adaptive composite ecological buffer zone is constructed, and the pollution interception and vegetation survival ability under the extreme climate is improved; biomass and low-pollution bottom mud in the drainage basin are subjected to resourceful treatment, the remediation cost is reduced, and secondary pollution is reduced; local functional florae are screened, and a slow-release microbial inoculum is prepared and added by adopting a composite embedding technology, so that the removal effect of refractory organic matters is enhanced; and multi-technology closed-loop regulation and control are realized through a digital twin model, and the repair process is dynamically optimized. Through multi-technology collaborative integration, the limitation of traditional fragmentation treatment is broken through, the ecological restoration effect and treatment economy of the drainage basin are remarkably improved, and the method is suitable for complex treatment scenes such as agricultural branch drainage basins.

The invention provides a preparation process of fen-flavor floating beam black tea, which comprises the following steps: constructing a multi-strain directional fermentation system consisting of debaryomyces hansenii, saccharomycopsis fibuligera and lactobacillus plantarum, and supplementing with seaweed kelose as a synergist; the problems of unstable quality and large flavor randomness caused by the fact that traditional black tea depends on natural microflora are solved. In the system, debaryomyces hansenii dominates to generate fresh and sweet floral compounds, saccharomycopsis fibuligera releases more aroma precursors by secreting extracellular enzyme and improves the mellowness of tea soup, and lactobacillus plantarum inhibits infectious microbes and optimizes the metabolic environment by producing acid; meanwhile, the seaweed kelose is used as an exclusive carbon source, so that rapid colonization and advantage establishment of functional flora are ensured. According to the technology, the faint scent type floating beam black tea with elegant and strong flower fragrance and fruit fragrance, fresh, refreshing and mellow taste and orange yellow and bright soup color can be finally and stably produced, and an effective path is provided for standardization and modernization upgrading of a traditional black tea technology.

The invention discloses a biological agent for preventing and treating panax notoginseng root rot, and relates to the technical field of biological prevention and treatment, the biological agent is composed of four layers of functional florae: an A layer of sentinel-decoding flora is used for identifying a pathogen quorum sensing signal and is used as an event trigger source, a B layer of metabolic countering flora is used for decoupling conversion of disease-promoting saponin and controllable release of a metabolic false substrate, and a C layer of functional florae is used for controlling root rot of panax notoginseng. The C-layer self-healing conductive biological membrane construction flora forms a biological membrane with conductivity and self-healing capability on the root surface, and the D-layer electrically-enabled flora constructs stable biological micro-current at the rhizosphere by using ferric oxide particles as external electron acceptors. The layer A triggers to cause controlled release of the layer B, a conversion product of the layer B provides a carbon source and a structural unit for the layer C, and a conductive network of the layer C and the layer D form an electronic path. The biological agent is used for early recognition and continuous inhibition of pathogens in panax notoginseng planting soil, can start defense response in the early stage of quorum sensing signal occurrence, and keeps action stability under complex environmental conditions.

The application discloses phenol high-efficiency degradation related strains and application thereof, and discloses strains with phenol degradation capacity, namely, Citrobacter farmeri with a preservation number of CGMCC No. 25400, Acinetobacter lwoffi with a preservation number of CGMCC No. 25399, Acidovorax soli with a preservation number of CGMCC No. 25478 and Diaphorobacter nitroreducens with a preservation number of CGMCC No. 25438. A method for degrading phenol by using the above strains is disclosed. The strains and the degradation method have the advantages of high efficiency, low consumption, simple operation, various treatment forms, less influence on the environment and no secondary pollution, and lay a foundation for the biological degradation of phenol.

The invention discloses bacillus subtilis Q16 and application thereof, the strain is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 20252232. According to the invention, the bacillus subtilis Q16 is separated from the body of the large yellow croaker and is added into the feed for the large yellow croaker, so that the growth performance, digestion, oxidation resistance and immunity of the large yellow croaker can be remarkably improved, and the intestinal flora composition structure of the large yellow croaker and the gene expression related to immunity and metabolism are changed.

To provide an NCP reference system for determining an NCP to be noted, a central processing unit, a terminal, a program, a storage medium, and a similar place NCP data extraction method.SOLUTION: The program causes the central processing unit to execute a step (S1) of receiving the target microbial flora association data from the terminal, a step (S2) of extracting the target microbial flora data from the target microbial flora association data, a step (S3) of calculating a similarity between the target microbial flora data and the reference microbial flora data, a step (S4) of extracting the reference microbial flora data having a high similarity by comparing the similarity with a similarity threshold, and a step (S5) of extracting the reference area microbial data included in the reference area data to which the reference microbial flora data having the high similarity belongs, as the similar area microbial data.SELECTED DRAWING: Figure 1

The present application relates to the technical field of excrement treatment, and particularly relates to a feces treatment method based on flora analysis, comprising obtaining reaction temperature and material Ph value and determining gas production relative deviation based on gas production, determining device operation state based on characteristic parameters; in response to the device operation state, determining abnormal characteristic identification to obtain key functional flora parameters, determining flora balance index based on the key functional flora parameters to determine whether acid-producing bacteria and methanogenic bacteria are in flora balance; determining smoothness index according to the change curve of a plurality of actual flora balance indexes obtained in an initial detection period to determine the deviation degree of the flora balance index, and adjusting the correction sensitivity coefficient of the feed adjustment amount based on the smoothness index. The present application performs real-time monitoring and dynamic regulation on the excrement treatment process based on the flora analysis result to maintain optimal flora structure and metabolic activity, and improves treatment efficiency and stability.

The application provides a preparation process of a Qingxiang type floating beam black tea. A multi-strain directional fermentation system composed of Hansenula debayii, Kuraishia sp. and Lactobacillus plantarum and supplemented with trehalose as a synergist is constructed to improve the problems of unstable quality and great flavor randomness of traditional black tea caused by relying on natural microbial flora. In the system, Hansenula debayii dominates the generation of sweet and sweet floral compounds, Kuraishia sp. releases more aroma precursors by secreting extracellular enzymes and improves the thickness of tea soup, and Lactobacillus plantarum inhibits miscellaneous bacteria and optimizes the metabolic environment by producing acid; at the same time, trehalose as a special carbon source ensures the rapid colonization and advantage establishment of the functional flora. The process can finally stably produce Qingxiang type floating beam black tea with high and elegant floral and fruity aroma, fresh and mellow taste, and bright orange-yellow soup color, and provides an effective path for the standardization and modernization upgrade of traditional black tea process.

The utility model relates to the technical field of flora separation equipment, in particular to a filter screen tightening and fixing assembly and a flora filter, the filter screen tightening and fixing assembly comprises a filter screen; the inner diameter circle of the upper cover is provided with a vertical surface; the outer diameter circle of the supporting bracket is provided with a binding face, and the binding face is matched with the vertical face so as to vertically clamp the outer edge of the tightened filter screen. According to the utility model, the filter screen can be clamped after being tightened, so that unfiltered florae are prevented from flowing out without being filtered.

The invention discloses an ecological living stone culture system and a method thereof, belongs to the field of aquarium engineering, and solves the problems of low seabed bacterium culture efficiency, non-uniform flora attachment, breeding of hybrid algae and poor ecological stability of the existing artificial living stone. The system comprises a base body, an internal artificial stone culture area, peripheral and top ecological pretreatment areas and a bottom biological sheltering area. The culture area is provided with a through water channel facing ocean current, independent compartments with grating bulkheads are arranged on the two sides of the culture area, a drainage plate and knocking holes are matched, the pretreatment area is provided with a seaweed culture base and a coral attachment plate, and the sheltering area is provided with stacked pipe fittings. During culture, firstly transplanting seaweed for ecological pretreatment, then putting algae-eating snails and hosting crabs, putting the artificial stones into the compartments for culture, and taking out the artificial stones until more than half of the surfaces of the artificial stones are covered with calcified algae. The device can realize uniform water supply, inhibit miscellaneous algae, shorten the cultivation period, adapt to submarine topography, facilitate large-scale cultivation and is suitable for coral reef restoration and aquarium ecological construction.

The bacterial strain Arasana bacillus (CECT 30655) belongs to Gram-positive bacterial flora and bacillus subtilis, and is a stimulant capable of stimulating adaptive metabolism of plants to resist water stress, an improving agent for plant yield and nutrition and an improving agent for polyphenol content. The strain is separated from rhizosphere soil of pinus coast, is cultured on a nutrient agar culture medium (PCA), and is identified as a new strain from the aspects of morphology, biochemistry and genetics for identification through whole genome sequencing. Under a water stress condition, the strain can enhance CO2 fixation and transpiration, optimize photosynthesis energy capture and reduce oxidative stress, so that the strain can be used for increasing yield; as a biological fertilizer, nutrient absorption can be promoted no matter whether water stress exists or not; and the content of antioxidant polyphenols in plant species with medicinal or edible values can also be improved, so that the fruit quality is improved.

The present application relates to the technical field of animal health monitoring, and relates to a wild animal non-invasive genome monitoring and population health evaluation system. A time indication marker is generated based on the ratio of a predetermined indicator bacteria genus, and microenvironment data is input into a pre-trained microbial succession model to intelligently infer the actual exposure time of the sample in the wild. Then, the initial intestinal flora map of the sample at the instant of discharge is reconstructed. The high-fidelity flora map after reconstruction is further deeply coupled with health evaluation to realize scientific diagnosis and early warning of the physiological state of wild animal individuals. The accuracy of health evaluation, nutritional status analysis and pathogen detection is greatly improved, and major misjudgments such as misjudging environmental bacteria as pathogenic bacteria or misdiagnosing flora natural decay as ecological imbalance are avoided. A stable and reliable technical tool is provided for wild animal protection management.

The utility model relates to the technical field of flora screening, in particular to a microbial flora screening device which can disperse and push flora on the upper surfaces of a plurality of screening pieces, ensures the screening effect, and is convenient to clean and use next time. Comprising a main body, a discharging pipe, a plurality of supporting legs, a plurality of screening pieces, a cleaning assembly, a mounting assembly, a limiting assembly and a dispersing assembly, the discharging pipe is connected to the right side of the bottom of the main body, the supporting legs are mounted at the bottom of the main body to support equipment, the cleaning assembly is mounted at the bottom of the main body, and the screening pieces are mounted in the main body through the mounting assembly; the bottom of the mounting assembly is connected with the cleaning assembly, the mounting assembly is connected with the top of the body through the limiting assembly, and the dispersing assembly is mounted on the screening part.

Lighting systems and methods of use. Such a lighting system has a carriage that carries an electric light and travels along an arcuate path to simulate the path of the sun over flora and / or fauna. A boom extends along the arcuate path, and the carriage travels along the boom. The carriage has a motor connected to a drive member operable to drive the carriage along the arcuate path. Optionally an adjustable timer may be used to set start and / or stop times for the carriage and / or the speed the carriage as the carriage travels along the arcuate path so that the lighting system can be adjusted to more closely simulate different natural sunlight conditions, such as day length, sunrise and / or sunset times, or even the period of an overall solar day cycle.

The invention provides a synthetic flora containing a complex microbial inoculant as well as a preparation method and application thereof, and relates to the technical field of microorganisms. The synthetic flora is composed of bacillus subtilis YX1 and pseudomonas marginata. Wherein the bacillus subtilis YX1 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.31921; the pseudomonas marginata is preserved in the same mechanism, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) 1.2844. After the synthetic flora is applied to the rhizosphere of ginseng, the synthetic flora has good environmental adaptability and rhizosphere survival ability. Through the combination of the specific functional strains, long-acting colonization of functional microorganisms in an aluminum stress environment is realized, and an effective scheme is provided for solving the problem of unstable colonization of microbial preparations in the ginseng planting process.

The invention belongs to the technical field of disease control in aquaculture, and particularly relates to a hippocampus enteritis pathogen separation and identification and medication screening method based on a high-throughput sequencing technology, which comprises the following steps: collecting intestinal tissues of a diseased hippocampus and a healthy hippocampus, respectively preparing tissue homogenate, and freezing for preservation; the method comprises the following steps: extracting DNA (Deoxyribose Nucleic Acid) of microorganisms of an intestinal tissue sample, performing 16SrDNA high-throughput sequencing, analyzing intestinal flora composition, and determining dominant bacteria; selecting and screening a culture medium according to dominant bacteria, and performing pathogenic bacterium separation and purification on intestinal tissues of the diseased hippocampus; dNA of the purified strain is extracted, 16S rDNA amplification and sequencing are carried out, and the species of pathogenic bacteria are identified through sequence comparison; according to the method, the diversity and abundance differences of microorganisms in the sick hippocampus and the healthy hippocampus are compared through a high-throughput sequencing technology, and targeted separation, identification and drug sensitivity screening are further carried out by taking statistically significant difference bacteria as the guidance of potential pathogenic bacteria.

The invention discloses a construction method of an animal model for depression caused by night illumination, and belongs to the technical field of animal model construction. Comprising the following steps: (1) selecting 8-week male C57BL / 6J mice as experimental animals, and dividing the experimental animals into a modeling group and a control group; (2) the modeling group simulates staying up late at night, and (3) animal behavioral depression experiments are respectively performed on the modeling group and the control group mice, and compared with the control group mice, the modeling group mice show typical depression behaviors, so that the animal model of depression caused by night illumination is obtained. The method has the beneficial effects that the animal model for the depression caused by the night illumination is successfully established by utilizing a controllable light night exposure experiment, the intestinal flora analysis is performed on excrement of a modeling group and a control group, and the change of the intestinal microbial flora induced by the abnormal night illumination is analyzed; a theoretical basis is provided for developing a rhythm intervention strategy of targeted intestinal microorganisms, and early prevention and precise treatment of depression are assisted.

The invention relates to an artificial domestication and cultivation method for Mongolian white mushroom based on micro-ecological simulation, and innovatively provides a micro-ecological simulation cultivation strategy aiming at the core technical bottlenecks that hyphae of Mongolian white mushroom grow extremely slowly, a conventional culture medium does not eat materials and fruiting is difficult. The method is characterized in that specific soil leaching liquor is prepared from mongolian white mushroom circle rhizosphere soil and added into a mother strain culture medium, and key microbial flora and growth factors for promoting hypha growth are successfully introduced; meanwhile, a special stock seed and a cultivation material which take fermented sheep manure and pasture root system granules as main materials are designed, and nutrients and physical structures of the original grassland ecology are accurately simulated. According to the method, the problem of hypha growth stagnation is thoroughly solved, the culture periods of mother seeds, stock seeds and cultivated seeds are remarkably shortened to 14 days, 22 days and 46 days from conventional 30 days or more, 40 days or more and 66 days or more, stable fruiting is successfully achieved, and the biotransformation rate is increased by 11.2% or more compared with that of a conventional method.

The invention discloses a method for culturing calculus bovis by utilizing flora, which is characterized in that cattle gall bladders obtained by slaughtering are used as reaction containers, so that the animal welfare problem caused by living body surgery is completely avoided. A plurality of key steps of bilirubin generation, cholesterol conversion, matrix formation and the like are synergistically completed by adopting functional complementary synthetic flora, and the components of the generated product are closer to a complex system of natural calculus bovis. The breeding is carried out in a closed bioreactor with controllable parameters, uncontrollable factors such as individual cattle difference and feeding environment are eliminated, and the product quality is highly stable and uniform. Automatic monitoring and feedback control are achieved in the whole cultivation process, and technological parameters are accurate and adjustable. The used strains are all non-pathogenic or food-grade safe strains, and the whole process is sterile, free of exogenous pathogen pollution and antibiotic residue risks, and high in biological safety.

The invention relates to a synthetic flora for degrading amygdalin and application of the synthetic flora in relieving amygdalin stress of peach trees. Through rhizosphere microorganism screening and functional verification, a strain, namely Sinomonas A7, which is enriched under the stress of mandelate in peach seedlings and has efficient degradation capability is identified, and the strain, Pristia megaterium C8 and Caballaria turbo P16 jointly form a synthetic flora with a synergistic effect. Experimental results show that the synthetic flora can effectively relieve the amygdalin-mediated autotoxicity effect of the plants, enhance the tolerance of the plants to stress and promote growth. The invention provides a technical route of the multifunctional complex microbial inoculant, and the multifunctional complex microbial inoculant has potential application values of removing autotoxic substances and improving crop resistance and productivity, and is suitable for rhizosphere improvement and health management of peach tree seedlings.

The invention discloses an esophageal squamous cell carcinoma noninvasive screening system based on a multi-marker saliva microbiome, and relates to the technical field of microorganisms and oncology, the key points of the technical scheme are as follows: the system comprises an input module, an analysis module and an output module, the input module inputs abundance change of specific flora of a saliva sample needing to be screened; the analysis module is used for analyzing the input saliva sample flora data; and the output module outputs the analysis result of the analysis module and judges whether the screened saliva sample owner belongs to the high-risk population of the esophageal squamous cell carcinoma patient. The abundance change of the specific flora of the saliva sample is obtained through metagenome sequencing or targeted PCR detection, and a microbial characteristic model is constructed to distinguish high-risk people. By means of the system, early-stage, non-invasive and high-acceptability screening of esophageal squamous carcinoma can be achieved.

This invention provides a method for improving the stress resistance of fallen larvae of the Oriental Wind Snail, relating to the field of Oriental Wind Snail farming technology. The farming process is as follows: (1) After cleaning the farming pond and exposing it to the sun, a layer of sieve cloth and sand is laid on the bottom of the pond in sequence, seawater is injected, and compound probiotic liquid is sprinkled; (2) The fallen larvae along with the attached sand are transferred to the farming pond. For the first 15 days after the transfer, they are fed compound feed, and after 15 days, they are fed compound feed and fresh feed; (3) During the farming period, an anti-stress agent is administered every 3 days. This invention guides the fallen larvae of the Oriental Wind Snail to adapt to the farming environment by controlling environmental conditions, reducing the stress impact caused by environmental changes; by using appropriate compound feed and anti-stress agents, the growth and development of the immune organs of the fallen larvae can be effectively promoted, as well as the balance of intestinal flora can be regulated, promoting the digestion and absorption of nutrients, improving metabolic efficiency, enhancing the body's immunity, thereby improving their stress resistance and effectively reducing mortality.

A mobile robot is programmed to travel through an agricultural area, such as an orchard of trees. The robot includes a camera for collecting two-dimensional image data associated with flora items in the agricultural area, and a light detection and ranging (LiDAR) system for detecting signals and collecting data indicative of a three-dimensional coordinate system of the agricultural area. The robot also includes a three-dimensional detection module programmed for generating clusters representative of flora items detected in the agricultural area, and for fusing portions of image data collected by the camera, data collected by the LiDAR system, and cluster data associated with the detected flora item. A correspondence module can be provided for matching detected flora items to a map of landmark flora item references.

The invention provides a restoration method based on black and odorous bottom mud in-situ chemical oxidation-biological synergy, and belongs to the technical field of environmental engineering. Comprising the following steps: adding a composite chemical oxidant into the black and odorous bottom mud to be repaired, reacting for a preset time, and adding a composite microbial agent into the same area; the complex microbial inoculant comprises microbial flora, growth factors and a composite carrier; wherein the microbial flora comprises pseudomonas putida, thiobacillus denitrificans, halomonas salina and rhodococcus erythropolis. The composite chemical oxidant provides rapid initial pollution reduction, and the composite microbial inoculant has efficient colonization ability, can perform long-term biodegradation and synergistic remediation, ensures the durability of the effect, solves the problem of mutual inhibition of chemical and biological processes, and is environment-friendly. The method is in-situ remediation, large-scale earthwork is not needed, the total cost is reduced, the method is completed mainly by adding chemicals, and the method is easy to apply and popularize in actual engineering.