37 results about "Coprophilous fungi" patented technology

The invention discloses a method for preparing earthworm cultivation substratum. By mixing livestock dung and edible fungus residue without foreign matters to carry out anaerobic fermentation, the method obtains the substratum for cultivating and reproducing earthworms, the livestock dung is pig dung or cow dung or the mixture of pig dung and cow dung, and the edible fungus residue is residue which is left over in the growth of cultured saprophytic lignicolous fungi or coprophilous fungi. Since the method utilizes the livestock dung and the edible fungus residue as the cultivation substratum to raise the earthworms, the method not only solves the problem of dung accumulation and waste and prevents environment pollution, but also increases the cultivation scale and growth quality of the earthworms by adding the edible fungus residue, thus effectively increasing the output of the earthworms.

The invention provides a coprophilous fungus composition. According to the invention, fresh night soil of adult health pig is taken as a manure source, a coprophilous fungus final suspension is prepared by three-time centrifugation under specific low-temperature condition, a high molecular weight compound protective agent skimmed milk powder and a low molecular weight compound protective agent (glycerin, lactose, galactose, cane sugar, glucose, maltodextrin, sorbitol, adonitol and trehalose) are added, the coprophilous fungus composition is produced, which contains abundant Bacillus spp, lactic acid bacteria, lactobacillus, bifidobacteria, and streptococcus thermophilus, live bacteria number is kept at 108 CFU / g and more, the coprophilous fungus composition is used for preventing and treating diarrhoea of pigling at a lactation period, the prevention and treatment effects are obvious, the coprophilous fungus composition has more obvious effect for preventing and treating diarrhoea of pigling with 0-14 days (21-28 days early-stage weaning method) and 0-21 days (35 days weaning method). The preparation method of the coprophilous fungus composition is simple, the product shelf-life is long, the defect that the current coprophilous fungus transplant requires real-time preparation in the prior art, and the coprophilous fungus composition is suitable for industrial production.

The invention discloses a preparation process of standard coprophilous fungi freeze-dried powder in transplantation therapy of coprophilous fungi. The preparation process comprises the following steps: (1) preparing standard coprophilous fungi liquid; (2) preparing a freeze-dried protecting agent; and (3) carrying out three-step-method freezing and vacuum drying, wherein the freeze-dried protecting agent is prepared by the steps of respectively adding 10%-20% of mycose, 5%-10% of glycerin and 0.02%-0.05% of vitamin C into sterile normal saline, uniformly mixing, filtering, and removing bacteria. According to the special freeze-dried protecting agent, the activity loss of the coprophilous fungi can be prevented, and the survival rate of the coprophilous fungi is increased; and by virtue ofa special freeze-drying procedure, the coprophilous fungi can rapidly enters a dormant state, so that the activity loss is avoided, and the stability of effective components is effectively protected.Experiments show that by utilizing the freeze-dried powder prepared by virtue of the preparation process, the activity of the coprophilous fungi in excrements is effectively maintained, and the survival rate of the coprophilous fungi is greatly increased.

The invention discloses a method for preventing and treating piglet diarrhea based on a caprophyl implantation technique. The method comprises the following steps that firstly, a healthy pig is adopted as a donor pig, and function flora in faeces of the donor pig is separated and purified; secondly, a piglet about to accept diarrhea prevention and treatment is adopted as a receptor piglet, the function flora is inoculated into the gastrointestinal tract of the receptor piglet, the intestinal flora with the normal function of the receptor piglet is built, and diarrhea prevention and treatment of the receptor piglet are achieved. The healthy biological female pig of the receptor piglet or other healthy piglets or other female pigs in the same house as the biological female pig of the receptor piglet can be adopted as the donor pig. The intestinal immune system can be fast built, the diarrhea of the piglet is effectively prevented from happening, the morbidity of piglet diarrhea can be controlled within two percent, growth and reproduction of pathogenic microorganisms or viruses in the intestinal tract can be effectively restrained, the aim of efficiently and thoroughly treating piglet diarrhea is achieved, and the cure rate is more than 95 percent.

The invention belongs to the technical field of excrement flora transplantation, and particularly relates to an excrement flora in-vitro culture method and a culture medium .The pH of the culture medium is 6.0-8.0 .The culture medium is prepared from peptone, yeast powder, a beef extract, glucose, cysteine, cholate, cellose, fructose, magnesium sulfate, monopotassium phosphate, vitamin K, ferrous sulfate, heme, a 1M CaCl2 solution and distilled water .The composition and the contents of the components of the culture medium determine that the content of probiotics, the proportion of bacillus and cocci, the proportion of gram-positive and negative bacteria and the like in flora obtained through culture are consistent with those of original flora, and by adopting the culture medium with the components and the ratio, flora consistent with fresh excrement flora in proportion can be well cultured .The adopted excrement flora in-vitro culture method is simple, the operation conditions can be accurately controlled, and the excrement flora prepared through the method is stable in proportion, good in repeatability, and suitable for industrial production .

The invention discloses a coprophilous fungus preservation solution and a method for preserving coprophilous fungi by adopting the preservation solution. The coprophilous fungus preservation solution is prepared from, by weight, 0.9% of sodium chloride, 0.5%-2.0% of vitamin C, 20%-50% of glycerinum and the balance sterile purified water, and the pH value of the preservation solution ranges from 7.0 to 7.4. According to the coprophilous fungi preservation solution and the method for preserving the coprophilous fungi, the coprophilous fungi can be preserved for up to three months under the simple preservation condition, and it is guaranteed that the microorganism composition is still similar to that of the preservation solution which is initially separated and extracted; from the angle of the formula composition, the advantages of being low in cost, easy to prepare and the like are achieved, and therefore development of coproghilous fungus treatment is facilitated; from the results of specific implementation cases, the coprophilous fungi can be kept basically invariable for up to three months under the conventional preservation condition, and therefore a main guarantee is supplied to coprophilous fungus transplantation standardization.

The invention discloses a disposable extraction device of target components of intestinal contents. The disposable extraction device comprises an extraction pipe, wherein the extraction pipe comprises a pipe body and a plurality of stages of filtering nets, wherein the upper end of the pipe body is provided with an opening and can be subjected to heat seal; the filtering nets are connected with the inner wall of the pipe body in a sealing manner; the pipe body also comprises liquid injection ends and liquid suction ends which are arranged on the lateral walls of the pipe body, wherein the liquid injection ends are positioned at the opening in the upper end and the first stage of filtering net, and the liquid suction ends are positioned between every two adjacent stages of filtering nets; the apertures of each filtering net from top to bottom have the trend of reducing stage by stage; the pipe body is made of a flexible high polymer material. The disposable extraction device disclosed by the invention has the beneficial effects that the structure is simple, the operation is convenient, an oxygen-free environment is provided for coprophilous fungi, the integrity of the coprophilous fungi can be guaranteed and the effect of the transplanted coprophilous fungi for treating diseases can be improved.

The invention discloses a method for preventing and treating diarrhea of poult based on a caprophyl implantation technique. The method comprises the following steps that firstly, healthy poultry are adopted as a donor, and function flora in faeces of the donor is separated and purified; secondly, the poult about to accept diarrhea prevention and treatment is adopted as a receptor, the function flora is inoculated into the gastrointestinal tract of the poult, the intestinal flora with the normal function of the poult is built, and diarrhea prevention and treatment of the poult are achieved. Other healthy poult or the biological female poultry of the poult as the receptor or other female poultry in the same house as the biological female poultry can be adopted as the donor. The normal intestinal microecology of the poult can be fast built, the intestinal immune system is built, the diarrhea of the poult is prevented from happening, growth and reproduction of pathogenic microorganisms or viruses in the intestinal tract can be effectively restrained, and the diarrhea of the poult is treated efficiently and thoroughly.

The invention discloses a method for extracting enteric microbial genome DNA. The method specifically comprises the following steps: (1) coprophilous fungi sample collection; (2) thallus acquisition;and (3) coprophilous fungi genome DNA extraction and purification. According to the method disclosed by the invention, usage of an organic solvent is reduced, the cost is low, and the extracted microbial total DNA has high purity; guanidinium isothiocyanate is capable of rapidly breaking cells and inhibiting ribozyme released from the cells, and the obtained DNA has high quality; enriched DNA population information can be obtained, the diversity and community composition of enteric microorganisms can be comprehensively reflected, the operating steps are less, the stability is excellent, and the method is a practical and reliable fecal microorganism DNA extraction method and can be widely applied to study on intestinal microecology.

The invention provides a coprophilous fungi positioning transplantation method. The method comprises the following steps: preparing capsules from coprophilous fungi serving as the content, orally taking the capsules, enabling the dissolved pH value of the capsule shells to adapt to the pH value of a target coprophilous fungi transplantation part, dissolving the capsules at the target transplantation part, and performing positioning transplantation on the coprophilous fungi at the target part. According to the method disclosed by the invention, the coprophilous fungi can be released at fixed points from the coprophilous fungi capsules according to different target transplantation parts according to the principle that different parts of human organs have different pH values, and the transplantation efficiency of the coprophilous fungi at the target transplantation part is improved.

The invention belongs to the technical field of disease biomarker screening and particularly relates to a method for screening a biomarker for diagnosing Alzheimer's disease. The method comprises thefollowing steps of (S1) respectively obtaining excrement from an Alzheimer's disease patient and a health reference person, (S2) respectively extracting coprophilous fungus outer membrane vesicles ofthe excrement obtained in the step (S1) and purifying, (S3) respectively carrying out GC-MS analysis on metabolites of the coprophilous fungi outer membrane vesicles purified in the step (S2) by usinga gas chromatograph-mass spectrometer, and (S4) analyzing the GC-MS data obtained in the step S3 and screening out a differential metabolite, namely the biomarker for diagnosing the Alzheimer's disease. The method has great significance to the diagnosis of the Alzheimer's disease.

The invention provides a drug-releasing capsule. Releasing of substances such as coprophilous fungi, therapeutic drugs, markers and food in the intestinal tract is remotely controlled. The drug-releasing capsule comprises a control cabin, a first drug-releasing cabin and a second drug-releasing cabin, and the control cabin comprises a wireless receiving module, a miniature battery and two microprocessors. After the drug-releasing capsule arrives at the gastrointestinal tract, when the control cabin receives in-vitro triggering signals for the first time, the first drug-releasing cabin releasesdrugs, and when the control cabin receives in-vitro triggering signals for the second time, the second drug-releasing cabin releases the drugs. By means of the drug-releasing capsule, the drugs can be released twice, and the drug-releasing capsule is free of invasion and pain and easy to operate.

The invention relates to the technical field of medical treatment, and particularly relates to a coprophilous fungi cryopreservation fluid and a preservation method thereof. The cryopreservation fluidcontains the following components in weight percentage by compounding: 1-5% of glycerin, 1-3% of tryptone, 0.1-0.5% of vitamin B, 0.7-0.9% of normal saline, and the balance of coprophilous fungi extracting soluiont. The coprophilous fungi cryopreservation fluid is cooled in match with a program, to control the coprophilous fungi cooling speed and time, so that the preservation time of the coprophilous fungi fluid at 80 DEG C is as long as more than 2 years, which is far higher than the storage time of coprophilous fungi whcih is 3-6 months in a general laboratory; and therefore, a solid foundation is laid for the storage of coprophilous fungi in a laboratory and the establishment of a coprophilous fungi specimen library.

The invention discloses a gellan gum oligosaccharide and application thereof in prebiotics and belongs to the technical field of biology. The invention provides the gellan gum oligosaccharide, and a basic structure of a main chain of the gellan gum oligosaccharide comprises a tetrasaccharide unit, wherein the tetrasaccharide unit comprises D-glucose, D-glucuronic acid and L-rhamnose, a molar ratio of the D-glucose to the D-glucuronic acid to the L-rhamnose is (4-6): (1.2-1.9): (2.4-3.6), and the polymerization degree range of the gellan gum oligosaccharide is 2-6. Through in-vitro static and dynamic coprophilous fungus fermentation methods, the novel gellan gum oligosaccharide is found to be capable of significantly increasing the yield of acetic acid, propionic acid, butyric acid and total SCFAs, improving the structure of fecal flora and significantly improving the relative abundance of butyric acid producing bacteria.

The invention relates to a directionally proliferating intestinal flora preparation for intestinal flora transplantation and a preparation method and application thereof, the directionally proliferating intestinal flora preparation comprises a GAM culture medium, an aloe-emodin solution, a PBS buffer solution and an intestinal microbial solution in a volume ratio of 2: 2: 1: (0.5-1.5), the concentration of the aloe-emodin solution is 9.93-10.13 g / mL, and the mass concentration of the intestinal microbial solution is 4-6%. According to the method, the intestinal environment is simulated in vitro, the intestinal flora collected from excrement of a healthy human body is taken as a sample, the aloe-emodin solution is applied to promoting proliferation and fermentation of probiotics, a scientific basis is provided for clinical application, the probiotics are efficiently prepared and applied to coprophilous fungus transplantation, the state of the intestinal flora can be effectively adjusted, and the survival rate of coprophilous fungus transplantation is increased. And constipation, Crohn and other inflammations are relieved, so that the morbidity of colon cancer is reduced.

The invention belongs to the field of medicines, and particularly relates to a traditional Chinese medicine formula flora capsule which is prepared by matching freeze-dried powder separated from coprophilous fungi and traditional Chinese medicine extraction powder obtained by crushing traditional Chinese medicine extraction effective components. The traditional Chinese medicine formula flora capsule is prepared by combining a traditional Chinese medicine extraction method and deeply fusing the traditional Chinese medicine extraction method with a coprophilous fungi separation process, the treatment effect is greatly improved while the administration method is changed, and an efficient and comfortable treatment way is realized.

The invention provides a fermentation method of human intestinal flora and application. The method comprises the following steps of a, donor screening, wherein boys who are twelve to fourteen years old, are in good health, do not have the serious disease history or obvious gastrointestinal tract symptoms, do not take any antibiotics in at least six months and have a regular life are screened out;b, sample collection and treatment, wherein fresh excrement of the donors is collected, quickly transferred to an anaerobic working station and weighed, a pre-reduction sterile saline solution is added according to a proportion, and even mixing with sufficient vortex is conducted; then filtering is conducted, and an excrement suspension solution is collected; c, fermentation, wherein the excrementsuspension solution is transferred to a sterile serum bottle, sealed and placed in a culture box for standing fermentation. According to the fermentation method, the obtained pH-acidity fermentationproduct with multiple rich probiotics is a kind of optimized healthy human intestinal flora and can be applied to the excrement bacterium transplantation technology and development of medicines, health care products and the like with the human intestinal flora as a target point.

The invention discloses application of teduglutide in preparation of a medicine for treating myocardial ischemia reperfusion injury. The teduglutide can protect the integrity of an intestinal barrier after myocardial ischemia, prevent intestinal flora displacement and intestinal flora imbalance, and relieve myocarditis reaction after myocardial ischemia reperfusion so as to treat the myocardial ischemia reperfusion injury. Compared with antibiotic therapy, probiotic intervention, coprophilous fungus transplantation and the like, the invention has a higher clinical transformation value and clinical application realizability. The invention discovers that the teduglutide has the effect of treating the myocardial ischemia reperfusion injury after myocardial infarction and can be used for preparing the medicine for treating acute myocardial infarction, and a new treatment scheme is provided for clinical treatment of the myocardial ischemia reperfusion injury.

The invention discloses an intestinal content extraction supervision device, which comprises: a box body, in which a heat insulation cavity is formed; the stirring module is arranged in the heat insulation cavity; the filtering and separating module is arranged in the heat insulation cavity and is connected with the stirring module; the filtering driving module is used for driving the solution in the filtering and separating module to circularly flow so as to automatically filter and separate; the temperature control module is arranged on the heat insulation cavity and used for adjusting and controlling the temperature in the heat insulation cavity; the low-oxygen module is communicated with the heat insulation cavity and is used for detecting and controlling the oxygen partial pressure value in the heat insulation cavity; an auxiliary module; the control module is in communication connection with the stirring module, the filtering driving module, the temperature control module, the low-oxygen module and the auxiliary module; and the terminal server is in communication connection with the control module. According to the intestinal content extraction supervision device, automatic extraction of intestinal contents can be realized, the measurement accuracy is ensured, the low-oxygen and constant-temperature requirements of a coprophilous fungi collection environment are ensured, communication with the control module can be realized in real time, and supervision is realized.

The invention provides application of cynanchum auriculatum in preparation of a product for promoting proliferation of intestinal beneficial bacteria, and relates to the technical field of microorganisms. According to the application, by simulating an intestinal environment in vitro and taking intestinal flora collected from faeces of a healthy human body as a sample, research finds that cynanchum auriculatum can remarkably promote proliferation of beneficial intestinal bacteria, particularly, the proliferation promotion effect on beneficial intestinal bacteria such as bifidobacteria, faecobacteria and Rosella is extremely remarkable, and in addition, the application has the advantages that the effect of promoting proliferation of the beneficial intestinal bacteria is obvious; the invention further provides a method for applying cynanchum auriculatum to directional fermentation of the specific donor flora for coprophilous fungus transplantation, so that the specific donor flora beneficial to the intestinal tract is obtained through directional fermentation, the specific donor flora can enter the gastrointestinal tract of a patient through coprophilous fungus transplantation, a new intestinal flora is reconstructed, and the effect of improving the intestinal tract is achieved. According to the application, a scientific basis is provided for clinical application, and the application field of cynanchum auriculatum is expanded.

The invention relates to a construction method of an animal model, in particular to a construction method of an animal model, and particularly relates to a method for establishing a hypertension yin-deficiency syndrome and / or hypertension non-yin-deficiency syndrome animal model by applying a coprophilous fungus transplantation method, an evaluation method and an application of the hypertension yin-deficiency syndrome and / or hypertension non-yin-deficiency syndrome animal model. A corresponding animal model with the hypertension yin deficiency syndrome and / or non-yin deficiency syndrome is obtained by preparing coprophilous bacteria liquid of a patient with the hypertension yin deficiency syndrome and / or non-yin deficiency syndrome and transplanting the prepared coprophilous bacteria liquid after a mouse takes a quadruple antibiotic drug in a drenching and drinking manner, and meanwhile, animal model evaluation is performed by adopting multiple methods to confirm whether the model is successfully established or not. The invention provides the hypertension yin-deficiency syndrome and / or non-yin-deficiency syndrome animal model which is high in stability, easy to popularize, good in repeatability, high in success rate, economical and practical, a basis is provided for research on traditional Chinese medicine treatment of hypertension, and a thought is provided for standardization and normalization of yin-deficiency syndrome model evaluation.

The invention provides an intestinal flora capsule and a preparation method thereof. The preparation method comprises the following steps: S10, preparing liquid; S20, centrifuging the fecal bacteria liquid, removing a supernate, retaining turbid liquid, and adding normal saline into the turbid liquid to obtain washing bacteria liquid; S30, mixing, centrifuging and washing the bacterial liquid for at least one time to obtain a purified bacterial liquid; and S40, preparing the purified bacterial liquid into powder, and filling the powder into capsules to prepare the intestinal flora capsules. According to the invention, the activity and quantity of intestinal flora can be maintained, the traditional endoscopic catheterization transplantation approach is improved, and a capsule oral administration mode is adopted, so that the coprophilous fungus transplantation approach is more convenient, and meanwhile, the treatment effect can also be ensured.

The present invention relates to a microbial composition for intestinal flora reconciliation comprising a strain of the genus Bifidobacterium, a strain of the genus Lactobacillus and / or a strain of other genus having substantially the same properties wherein the strain of the genus Bifidobacterium is selected from the group comprising Bifidobacterium breve, Bifidobacterium infantis and Bifidobacterium longum. The coprophilous fungus transplantation is a powerful means for achieving the purpose of disease treatment by adjusting the intestinal microecology. The transplanting components of coprophilous fungi are too complex, and quality controllability and accurate intervention cannot be realized. Therefore, through intestinal flora data mining and analysis, several core strains playing an important role in ecology are selected to realize regulation and control of the whole intestinal micro-ecology, so that alleviation and treatment of disease symptoms are realized.

The invention discloses a coprophilous fungus freeze-drying protective agent and application thereof in coprophilous fungus transplantation, and belongs to the technical field of biology. The coprophilous fungus freeze-drying protective agent is prepared from the following components: 5%-15% of trehalose, 5%-25% of edible substances and the balance of sterile water. Edible substances are adopted,through a reasonable formula, the survival rate of microorganisms in the freeze-dried powder can be stabilized, and the activity of intestinal flora can be protected; the freeze-dried powder technology is adopted, implementation of flora transplantation is facilitated, and operation is convenient.

The invention discloses a coprophilous fungus cryoprotectant and application thereof in coprophilous fungus preservation, and the coprophilous fungus cryoprotectant is prepared from the following components in percentage by mass: 20%-36% of trehalose, 14%-22% of cane sugar, 4%-8% of skim milk powder, 4%-8% of betaine, 0.02%-0.06% of L-cysteine hydrochloride, 0.3%-0.7% of D-sodium erythorbate, 0.1%-0.2% of glutathione and the balance of water. And the balance of sterile water. The coprophilous fungus cryoprotectant disclosed by the invention can effectively protect the initial composition proportion, flora diversity and bacterium survival rate of coprophilous fungus suspension under a low-temperature freezing condition. According to the coprophilous fungus cryoprotectant disclosed by the invention, the flora composition of a frozen bacterial suspension is closer to that of a bacterial suspension before freezing, and the coprophilous fungus cryoprotectant is of great significance for scientific researchers to accurately research intestinal flora composition.

The invention provides application of coptis chinensis in preparation of a product for promoting proliferation of intestinal beneficial bacteria, and relates to the technical field of microorganisms. According to the application, an intestinal environment is simulated in vitro, an intestinal flora collected from faeces of a healthy human body is taken as a sample, and research finds that coptis chinensis can significantly promote proliferation of intestinal beneficial bacteria, especially has an extremely significant effect of promoting proliferation of intestinal beneficial bacteria such as bifidobacterium and Arrisonia; the invention further provides application of coptis chinensis in preparation of drugs for treating or improving diseases caused by intestinal flora imbalance. Particularly, the coptis chinensis can be applied to a method for directionally fermenting the specific donor flora for coprophilous fungus transplantation, so that the specific donor flora beneficial to the intestinal tract is directionally fermented, and the specific donor flora can enter the gastrointestinal tract of a patient through coprophilous fungus transplantation to reconstruct a new intestinal flora, so that the treatment of intestinal and extraintestinal diseases is realized; scientific basis is provided for clinical application, and the application field of coptis chinensis is expanded.

The invention discloses a disposable extraction device of target components of intestinal contents. The disposable extraction device comprises an extraction pipe, wherein the extraction pipe comprises a pipe body and a plurality of stages of filtering nets, wherein the upper end of the pipe body is provided with an opening and can be subjected to heat seal; the filtering nets are connected with the inner wall of the pipe body in a sealing manner; the pipe body also comprises liquid injection ends and liquid suction ends which are arranged on the lateral walls of the pipe body, wherein the liquid injection ends are positioned at the opening in the upper end and the first stage of filtering net, and the liquid suction ends are positioned between every two adjacent stages of filtering nets; the apertures of each filtering net from top to bottom have the trend of reducing stage by stage; the pipe body is made of a flexible high polymer material. The disposable extraction device disclosed by the invention has the beneficial effects that the structure is simple, the operation is convenient, an oxygen-free environment is provided for coprophilous fungi, the integrity of the coprophilous fungi can be guaranteed and the effect of the transplanted coprophilous fungi for treating diseases can be improved.