1 results about "Cynanchum" patented technology

The application discloses a method for rooting induction of Cynanchum coccineum Schneid. tissue culture seedlings, and comprises the following steps: inoculating Cynanchum coccineum Schneid. tissue culture seedlings into MS modified culture medium to obtain sterile seedlings; inoculating the sterile seedlings into a proliferation culture medium for proliferation culture for 28-35 days to obtain adventitious bud clusters; inoculating the adventitious bud clusters into a rooting induction culture medium for rooting induction to obtain Cynanchum coccineum Schneid. seedlings; wherein the MS modified culture medium is a basic culture medium added with 0.1-0.2 g / L of activated carbon; the proliferation culture medium is a basic culture medium added with 0.2-1.0 mg / L of IBA, 0.1-0.5 mg / L of IAA and 0.3-1.0 mg / L of 6-BA; and the rooting induction culture medium is a basic culture medium added with 0.5-2.0 mg / L of NAA or 0.5-2.0 mg / L of IBA. The application can quickly induce Cynanchum coccineum Schneid. seedlings, and solves the problems of rooting difficulty and introduction and cultivation difficulty of Cynanchum coccineum Schneid. tissue culture.