75 results about "Calculus bovis" patented technology

The invention belongs to the technical field of biological agriculture, and particularly relates to a method for promoting growth of natural calculus bovis by using a novel small molecule peptide combination, which comprises the following steps: selecting a plurality of small molecule peptides with specific functions, and mixing the small molecule peptides according to a specific proportion to form a small molecule peptide combination with a synergistic effect. The growth of the natural calculus bovis can be effectively promoted, and the yield and the quality of the natural calculus bovis are improved. Meanwhile, the method disclosed by the invention has relatively high safety and has a wide prospect in practical application. Animal experiment data further verifies the effectiveness and safety of the method.

The invention discloses a preparation method and application of a Tibetan medicine Qipeng pill. The Qipeng pill comprises Tibetan medicine active components and microcrystalline fibers, the microcrystalline fibers account for 30% of the weight of pellets, and the method comprises the following steps: uniformly mixing the active components and the microcrystalline fibers to obtain a mixture, adding 75% ethanol to prepare a soft material, filling the soft material into an extruder, extruding the soft material into strip columns, cutting the strip columns in a rolling machine, rolling the strip columns into pellets with uniform sizes, drying and sieving to obtain the Qipeng pill. Wherein the active component is prepared from fine powder of seven raw materials including fructus chebulae (denucleated), radix aconiti lateralis preparata, radix aucklandiae, rhizoma acori calami, Muslim myrrh, calculus bovis factitius and artificial musk. The Qipeng pill prepared by the method is good in roundness, good in friability, high in yield and uniform in size. In addition, the Qipeng pill also has the prospect of treating acute lung injury.

The invention discloses a flavoring preparation method of Wanchu granules, and belongs to the field of medicine preparation, and the flavoring preparation method comprises the following steps: S1, forming first mixed powder from rhizoma picrorhizae, rhizoma coptidis, catechu and fragrant ink; s2, in-vitro cultured calculus bovis is mixed with the first mixed powder to form second mixed powder; s3, forming bear gall powder liquid; s4, forming ox gall paste; s5, forming a third mixture from the ox gall paste and the bear gall powder liquid; s6, mixing the rape flower honey filtrate, the second mixed powder and the third mixture, granulating through a granulator, drying and finishing; and S7, mixing the borneol and artificial musk particles obtained in the step S6. According to the invention, not only is the taste modifying effect of the Wanchu granules greatly improved, but also the heat clearing effect of the Wanchu granules is improved.

The invention belongs to the technical field of biology, and particularly relates to a small molecule peptide composition and method for regulating natural calculus bovis production. The small molecule peptide aiming at a key target spot is designed by deeply researching secretion and metabolism of ox bile. The action mechanism covers biliary secretion stimulation, component optimization, bezoar key enzyme activity promotion and immune response regulation. A chemical synthesis or biotechnology means is adopted for preparation, and the dosage is determined according to cattle conditions during application. The safety is strictly evaluated, the yield of the natural calculus bovis can be obviously improved by 65%, the quality is also greatly improved, the effective components are increased, the impurities are reduced, the drug effect is enhanced, a new efficient and safe way is opened up for the production of the natural calculus bovis, and the method has a wide application prospect.

The invention relates to a preparation method of bionic calculus bovis, the bionic calculus bovis and application of the bionic calculus bovis, and belongs to the technical field of traditional Chinese medicine raw material preparation. According to the preparation method, natural oxgall is used as a fermentation substrate, a fermentation and / or enzymatic reaction system is used for preparing the bionic calculus bovis, and the fermentation and / or enzymatic reaction system is selected from at least one of synthetase plasmids, synthetase high-expression engineering bacteria and calculus bovis forming bacteria as a fermentation strain for direct in-vitro fermentation. The strains of the bezoar forming bacteria, the carriers of the synthetase high-expression engineering bacteria and the specific types of the synthetase plasmids are described in the specification. The bionic calculus bovis prepared by the method is highly consistent with natural calculus bovis in component, the content of cholic acid (CA) is 8.5%-20.0%, the content of deoxycholic acid (DCA) is 2.0%-6.5%, the fingerprint similarity is greater than or equal to 88.0%, and pharmacological experiments show that the bionic calculus bovis prepared by the method has the same acting effect as the natural calculus bovis and is superior to that of each pair of calculus bovis.

The invention discloses a method for comparing depression treatment effects of calculus bovis heart-clearing pills. By using the method disclosed by the invention, the component information and the action mechanism of the (local formula) bezoar heart-clearing pill for treating the depression can be explained, a theoretical basis is provided for clinically applying the (local formula) bezoar heart-clearing pill for treating the depression, and the treatment effects of the (local formula) bezoar heart-clearing pill and the existing other bezoar heart-clearing pills on the depression can be compared by using the method.

The invention relates to the technical field of traditional Chinese veterinary medicines, in particular to a veterinary antiviral traditional Chinese medicine composition and a preparation method thereof.The traditional Chinese medicine composition is prepared from, by weight, 20-40 parts of gypsum and 15-30 parts of rhizoma anemarrhenae; 15-30 parts of cornu bubali; 10-15 parts of manyleaf paris rhizome; 10-15 parts of radix paeoniae rubra; 10 to 15 parts of moutan bark; 10 to 15 parts of uncaria rhynchophylla; 0.3-1 part of calculus bovis factitius; 15 to 30 parts of magnet; 1-3 parts of benzoin; 10-15 parts of radix trichosanthis; and 10-15 parts of cape jasmine. The preparation method comprises the following steps: decocting the traditional Chinese medicines to prepare traditional Chinese medicine decoction, and concentrating overnight filtrate under reduced pressure to obtain traditional Chinese medicine fluid extract; and adding clinically acceptable auxiliary materials into the traditional Chinese medicine fluid extract, and preparing a clinically acceptable oral traditional Chinese medicine preparation by adopting a conventional preparation mode. The veterinary antiviral traditional Chinese medicine composition provided by the invention has a good treatment effect on viral diseases of livestock according to the principle of dialectical treatment of traditional Chinese veterinary medicines, can be used for replacing western medicines, and effectively avoids the problems of food safety and drug resistance caused by drug residues of the western medicines.

The invention belongs to the technical field of in-vitro cultured calculus bovis, and provides in-vitro cultured calculus bovis and a preparation method thereof. The preparation method comprises the following steps: (1) separating cells; (2) primary culture; (3) cell inoculation and cultivation: adding the serum-free culture medium into the carrier matrix, putting the carrier matrix into a reactor, and performing cell inoculation on the carrier matrix after passage; the inoculated culture comprises a proliferation period; a synthesis period; a crystallization period; and (4) collecting and purifying the calculus bovis, namely stopping culturing after 45 days, and sequentially collecting, cleaning, degreasing, drying and crushing the calculus bovis. The preparation method aims at solving the problems of long culture period, unstable content of effective components, high production cost, large batch difference and the like in the existing in-vitro calculus bovis culture technology by optimizing a culture medium formula, regulating and controlling a culture process and a purification process.

The invention belongs to the technical field of traditional Chinese medicine (Tibetan medicine), and particularly relates to a Tibetan medicine composition for treating new coronavirus infection and application of the Tibetan medicine composition. The invention discloses a Tibetan medicine composition for treating new coronavirus infection and application of the Tibetan medicine composition. The composition is prepared from the following components in parts by weight: 100 to 700 parts of fructus chebulae, 10 to 80 parts of radix aconiti lateralis, 10 to 60 parts of radix aucklandiae, 50 to 600 parts of rhizoma acori calami, 10 to 50 parts of Muslim myrrh, 0.01 to 50 parts of calculus bovis factitius and 0.01 to 50 parts of artificial musk. The composition can inhibit the mRNA expression of the N gene and the ORF1ab gene after the SARS-CoV-2 virus infects the Vero cell, so that the treatment on the novel coronavirus infection is realized.

The invention discloses an application of a traditional Chinese medicine composition in preparation of a medicine for preventing and treating chronic heart failure with cognitive impairment. The traditional Chinese medicine composition comprises the following components: ginseng, lucid ganoderma, safflower, musk, calculus bovis, bear gall, pearl, venenum bufonis, radix aconiti carmichaeli and borneol. The traditional Chinese medicine composition has a good treatment effect on qi deficiency and blood stasis type chronic heart failure accompanied with cognitive impairment, and a large number of test data prove that the traditional Chinese medicine composition can effectively improve the heart function, and / or cognitive function and / or traditional Chinese medicine symptoms of qi deficiency and blood stasis type chronic heart failure accompanied with cognitive impairment through reasonable compatibility of all the components, so that the traditional Chinese medicine composition has a good curative effect on qi deficiency and blood stasis type chronic heart failure accompanied with cognitive impairment. The symptoms of chronic heart failure with cognitive impairment are relieved from multiple aspects, and the progress of the disease course is blocked. The prescription is safe and reliable without toxic and side effects on human bodies.

The invention provides a high-fidelity calculus bovis and a preparation method thereof, and belongs to the technical field of traditional Chinese medicines. According to the technical scheme provided by the invention, the high-fidelity calculus bovis is accurately prepared by taking the mean value of the contents of various components of the natural calculus bovis as a reference and accurately measuring the contents of various components in the raw materials, fully utilizing the original components of the ox bile or ox gall powder, not adding other non-calculus bovis components and combining the existence forms of the components. The problems that existing artificial calculus bovis is greatly different from natural calculus bovis in efficacy, and toxic and side effects are relatively high are solved. The shape, the forming process and the components of the product are highly similar to those of natural calculus bovis; the medicine effect of the calculus bovis is improved, and the production cost is reduced.

The invention discloses a traditional Chinese medicine manufacturing knowledge extraction method and device based on a small-sample fine-tuning large language model and prompt engineering and electronic equipment. The method comprises the following steps: firstly, acquiring low-quality non-standard real text data in the field of manufacturing of the same-kernel calculus bovis heart-clearing pills, and generating a high-quality standard text by utilizing a large language model to construct a mixed evaluation set; then, a few-sample training set is constructed through human-in-loop mechanism iteration, fine adjustment is carried out on a GPT-4omini model through OpenAI API, and fine adjustment parameters comprise the learning rate 1e-4, the batch size 5 and the training round number 5; meanwhile, a structured prompt template for clearly specifying entity types and relationships is constructed; and after model reasoning, automatically outputting a structured result in a JSON-LD format, analyzing the structured result, and storing the analyzed structured result in a Neo4j graph database to construct a knowledge graph. According to the method, through combination of few-sample fine adjustment and prompt engineering, the sensitivity to data quality is remarkably reduced, and the knowledge extraction problem of non-standard texts with professional term nesting and structure breakage in the field of traditional Chinese medicine manufacturing is effectively solved.

The invention belongs to the technical field of biochemical pharmacy, and particularly relates to a compound bezoar composition, decoction piece type compound bezoar and a preparation method of the decoction piece type compound bezoar. The compound calculus bovis composition provided by the invention comprises 94-96% by mass of main agents and 8 kinds of calculus bovis intermediates, the mass content of the auxiliary agent is 4-6%, and the auxiliary agent is six commercially available finished product combined cholic acid. The bilirubin content of the bezoar intermediate used in the invention is increased by more than 30% and the yield is increased by more than 25% compared with the existing biochemical reaction process of oxgall. The DPPH free radical scavenging rate of the compound bezoar composition reaches 89.6% + / -2.1% and is increased by 25.7-36.9% compared with 58.3% + / -3.5% of a product prepared from ox bile, and through t inspection, p is smaller than 0.01 (n is equal to 5). Free cholic acid intervention is strictly prohibited, lithocholic acid addition is completely eradicated, and the composition has the advantages of being high in bilirubin content, high in oxidation resistance and high in biological activity and adapts to the physiological characteristics of the human body.

The invention relates to the technical field of preparation and mixing, and discloses a preparation stirring device and a preparation method for mass production of natural calculus bovis. Through continuous three-time stirring configuration of the pre-mixing cavity, the primary configuration cavity and the secondary configuration cavity, and through the primary conveying unit, the primarily stirred and mixed liquid is pressurized and conveyed into primary liquid on the upper layer and the lower layer in the primary configuration cavity; the liquid stirred and mixed in the primary configuration cavity is pressurized and conveyed to the interior of the liquid of each layer in the secondary configuration cavity through the secondary conveying unit, so that the liquid of the previous stage is dispersed in the configuration liquid of the next stage after being stirred and configured in a mixed state; and the interiors of the premixing cavity, the primary configuration cavity and the secondary configuration cavity can be fully stirred and mixed more uniformly, and the configuration uniformity degree can be more effectively guaranteed.

The invention discloses a traditional Chinese medicine composition for inhibiting and fading scar hyperplasia and a preparation method of the traditional Chinese medicine composition, and belongs to the field of traditional Chinese medicine compositions for fading scars. 5 to 15 parts of a gel matrix; 10 to 25 parts of an oil phase component; 3-8 parts of an emulsifier and a thickener; and the balance of water. Wherein the active traditional Chinese medicine component consists of 1-5 parts of calculus bovis factitius, 1-3 parts of artificial musk, 3-10 parts of cowherb seed, 3-10 parts of golden cypress, 2-8 parts of frankincense and 2-8 parts of myrrh. The traditional Chinese medicine composition for inhibiting and fading scar hyperplasia and the preparation method of the traditional Chinese medicine composition have the beneficial effects that the traditional Chinese medicine composition for inhibiting and fading scar hyperplasia and the preparation method of the traditional Chinese medicine composition are provided, all the medicines are combined to jointly achieve the effects of promoting blood circulation to remove blood stasis, dredging collaterals, removing stasis, resisting inflammation and promoting tissue regeneration and directly refer to the pathogenesis core of scar hyperplasia, and an oil-in-water (O / W) latex dosage form is adopted, so that the traditional Chinese medicine composition has the refreshing effect of gel and the moistening effect of cream, and is easy to coat and good in air permeability.

The invention relates to the technical field of traditional Chinese medicines, in particular to a concentrated heart-activating pill and a preparation method thereof. The preparation method comprises the following steps: weighing raw materials including lucid ganoderma, artificial musk, bear gall, calculus bovis, pearl, ginseng, venenum bufonis, radix aconiti carmichaeli, borneol, safflower and starch; ganoderma lucidum, ginseng, venenum bufonis, radix aconiti carmichaeli and safflower are smashed to obtain powder, water is added for boiling, heat preservation and stirring are conducted, and a first intermediate product is obtained; adding fig protease and ginger protease into the intermediate product I, and performing enzyme deactivation to obtain an intermediate product II; sterilizing the intermediate product II, adding a carbon source, a nitrogen source and zymophyte, fermenting, sterilizing and filtering to obtain an intermediate product III filtrate; adding artificial musk, bear gall, calculus bovis, pearl, borneol and starch into the intermediate product III, stirring, concentrating under reduced pressure, kneading pills, and drying to obtain the concentrated heart-activating pills. The concentrated active pill prepared by the invention has an excellent blood fat reducing effect, and has the effects of remarkably reducing the TC, TG and LDL-C contents in serum and effectively increasing the HDL-C content.

The invention discloses a bezoar quality improving method based on natural pasture environment optimization. According to the method, the internal quality of the calculus bovis is remarkably improved through multi-dimensional cooperative regulation and control. A natural feed basis with balanced nutrition is provided for cattle from soil environment baseline investigation to precise pasture proportioning, and by combining directional cultivation and implantation of genetic engineering strains, the metabolic activity of rumen microorganisms is effectively enhanced, and accumulation of key components for synthesis of calculus bovis is promoted. The linkage application of the intelligent monitoring system and the environment regulation and control device optimizes the living environment of cattle in real time, and cooperates with innovative intervention means such as lavender essential oil and music therapy, so that the stress reaction of cattle herds is remarkably reduced, and the efficient operation of liver metabolic pathways is guaranteed. The directional feeding of the functional lick bricks further enhances the conversion efficiency of the calculus bovis precursor substance, the whole-process data tracing ensures the controllability of the quality formation process, and finally the improvement of the content of effective active ingredients in the calculus bovis and the enhancement of the quality stability are realized.

The invention belongs to the technical field of microorganisms, and particularly relates to a bacterial strain for promoting formation of calculus bovis, a microbial agent and application of the bacterial strain, the bacterial strain is classified and named as Escherichia fergusonii, the bacterial strain is preserved in China General Microbiological Culture Collection Center on May 13, 2025, and the preservation number is CGMCC No.34526. The invention further discloses a preparation method of the bacterial strain for promoting formation of calculus bovis. The strain can be used as a transformation strain for in-vitro cultivation of calculus bovis and can also be used as an implantation strain for artificial in-vivo cultivation of calculus bovis, and the problems that in the process of artificial in-vitro cultivation or in-vivo cultivation of calculus bovis, thalli are low in bile tolerance and enzyme production activity, short in survival time in ox bile and low in calculus bovis forming efficiency are solved.

The invention discloses eye protection liquid and a preparation method thereof, and belongs to the technical field of traditional Chinese medicine external preparations. The eye protection liquid is prepared from a chrysanthemum extract, borneol, calculus bovis factitius, venenum bufonis, realgar, a mint extract, a pipewort extract, a feather cockscomb seed extract, a butterflybush flower extract, pearl powder and purified water according to a specific weight part ratio, and is prepared by soaking, distilling, filtering and filling. The traditional Chinese medicine composition can rapidly permeate into eyes and directly enter focus, can relieve and treat various eye diseases such as presbyopia, myopia, amblyopia, cataract and the like, has the effects of soothing liver and relieving depression, promoting blood circulation and dredging collaterals, tonifying kidney and nourishing yin, supplementing optic nerve nutrition and improving microcirculation around the eyes, has the total effective rate of 100% and the cure rate of 80-95%, and is free of side effects and drug resistance and convenient to use.

The utility model relates to the field of injectors, in particular to a bezoar strain injector with a leakage-proof design. Comprising a needle cylinder, a piston is installed on the inner wall of the needle cylinder, a needle head is installed at one end of the needle cylinder, an adjusting structure is arranged on one side of the piston and comprises a connecting block, the connecting block is fixedly connected with the piston, a connecting plate is fixedly connected to one side of the connecting block, and a sliding groove is formed in the upper surface of the connecting plate; a positioning block is slidably connected to the inner wall of the sliding groove, a fixing hole is formed in the upper surface of the positioning block, a fixing rod is slidably connected to the inner wall of the connecting plate, and a limiting plate is fixedly connected to one end of the fixing rod. The calculus bovis strain injector with the leakage-proof design has the advantages that the position of the piston can be effectively locked, the piston is prevented from moving due to external factors in the operation, transportation or storage process, the safety of strains is ensured, and the leakage phenomenon is avoided.

The invention discloses a traditional Chinese medicine composition for treating radiation enteritis, a suppository and a preparation method and application thereof. The traditional Chinese medicine composition for treating radiation enteritis comprises the following components in parts by weight: 6-15 parts of pseudo-ginseng powder, 6-15 parts of rhizoma bletillae, 1-7 parts of dragon's blood, 1-5 parts of calculus bovis factitius, 6-15 parts of pearl powder, 6-15 parts of dried ginger charcoal, 6-15 parts of rheum officinale charcoal, 20-50 parts of dandelion, 20-50 parts of sophora flower, 10-30 parts of coptis chinensis and 30-80 parts of honey. The traditional Chinese medicine composition can clear away heat and toxic materials, cool blood, stop bleeding, promote tissue regeneration and heal sores, is prepared into an external preparation, and can effectively treat 1-3 level radiation enteritis through external application of the traditional Chinese medicine composition for clearing away heat and toxic materials and promoting tissue regeneration and heal sores. Compared with oral traditional Chinese medicines, the external medicine disclosed by the invention has the advantages that the first-pass effect of the liver is avoided by intestinal administration, and the utilization efficiency of the medicine is improved. The suppository is used instead of traditional enema, so that the contact time between the traditional Chinese medicine and the diseased region of the intestinal tract is prolonged, and the curative effect of the traditional Chinese medicine can be better exerted.

The invention relates to a method for detecting the content of arsenic in quick-acting calculus bovis pills and application thereof, and belongs to the technical field of traditional Chinese medicine detection. The invention provides a method for detecting the content of arsenic in quick-acting bezoar pills, which comprises the following steps: mixing the quick-acting bezoar pills, nitric acid and hydrochloric acid, digesting, and adding water to a constant volume to obtain a diluent; mixing the acid solution and the diluent to obtain a sample solution; and measuring the absorbance of the sample solution by adopting an atomic absorption spectrophotometric method, and calculating the content of arsenic in the quick-acting calculus bovis pill according to the standard curve. The detection method provided by the invention can accurately detect the content of arsenic in the quick-acting calculus bovis pill, and has the characteristics of high specificity, repeatability, stability and accuracy.

The invention discloses a composition for relieving chronic pain related depressive mood. The composition is mainly prepared from the following traditional Chinese medicinal materials in parts by weight: 5-30 parts of borneol, 5-30 parts of musk, 50-100 parts of liquorice, 50-100 parts of burdock, 50-100 parts of gardenia kernel, 20-60 parts of calculus bovis, 50-100 parts of acorus gramineus and 50-100 parts of radix curcumae. Molecular mechanisms and potential treatment effects of borneol powder for treating chronic pain related depressive mood are researched by a modern scientific method, and safety and effectiveness of medicines are evaluated by pharmacodynamic and toxicological evaluation and animal experiments, so that the traditional Chinese medicine composition capable of effectively relieving chronic pain related depressive mood is provided.

The invention discloses a pure traditional Chinese medicine for treating acute and chronic liver diseases and a preparation method thereof, and belongs to the technical field of liver disease treatment. The pure traditional Chinese medicine preparation comprises the following components in parts by weight: 1-10 parts of vinegar tortoise plastron, 1-5 parts of American ginseng, 1-6 parts of Sichuan yellow, 1-3 parts of donkey-hide gelatin, 1-4 parts of cordyceps sinensis, 1-5 parts of lucid ganoderma, 1-4 parts of prinsepia utilis royle, 1-3 parts of olean, 1-4 parts of cedar parasitism, 1-3 parts of parasitic loranthus, 1-5 parts of grifola, 1-4 parts of couch grass root, 0.5-3 parts of saffron crocus, 1-4 parts of angelica sinensis, 1-4 parts of polygonum cuspidatum, 1-5 parts of penthorum chinense pursh, 0.5-3 parts of calculus bovis factitius and 1-4 parts of radix paeoniae alba. The traditional Chinese medicine composition is prepared from 1-3 parts of oregano, 1-4 parts of fructus lycii, 1-3 parts of schisandra chinensis, 1-4 parts of fructus gardeniae, 1-4 parts of artemisia capillaris, 1-3 parts of dandelion, 1-3 parts of safflower, 1-4 parts of abrus herb, 1-3 parts of selfheal, 1-3 parts of verbena, 1-3 parts of fennel, 1-3 parts of tangerine seed and 1-4 parts of liquorice. According to the invention, through accurate component proportion, the optimal combination of the medicinal components is ensured, the curative effect and safety of the medicine are improved, the preparation process of the traditional Chinese medicine is simplified, and the extraction and purification degree of the medicinal components is improved.

The invention discloses a compound paracetamol and amantadine hydrochloride capsule containing caffeine microcapsules, which relates to the technical field of medicines and comprises paracetamol, amantadine hydrochloride, calculus bovis factitius, pharmaceutic adjuvants and functional microcapsule units. The functional microcapsule unit has a three-layer structure: a core material taking caffeine and microcrystalline cellulose as a core, an ethyl cellulose controlled release layer and a quick release layer containing chlorpheniramine maleate. According to the structure, time sequence controlled release of drugs is achieved by synthesizing a polymer coating material, chlorpheniramine maleate is quickly released when encountering gastrointestinal fluid to quickly resist allergy, and caffeine starts to be slowly released after being effectively delayed to the intestinal environment, so that the time when the plasma concentration reaches the peak tends to be synchronous with the time when chlorpheniramine maleate reaches the peak; the drowsiness side effect can be effectively resisted.

The invention discloses a method for cultivating calculus bovis in ox gall induced by solvent exchange phase change gel. The problem that calculus bovis is not suitable for cultivation in the prior art is solved. The method comprises the following steps: S1, selecting calves, wherein the calves are 4-8-month-old calves; s2, mixing 45%-55% of egg yolk lecithin, 12%-18% of medium-chain and long-chain fatty acid, 18%-22% of ethyl cellulose and the balance of ethyl alcohol to prepare an injectable solution; s3, injecting 6 to 12 ml of the injectable solution into the ox gall; s4, injecting calculus bovis conversion flora to infect the cattle; and S5, after the cattle are normally fed for 1-1.5 years, killing the cattle and taking yellow. After the injectable solution is injected into the ox gall, water in bile exchanges with ethanol in the preparation, the injectable solution is gradually and completely converted into a gel state from a solution state after 8 hours and exists in the ox gall, and the injectable solution is gradually absorbed and metabolized along with gradual absorption and metabolism of egg yolk lecithin; bezoar crystals are gradually separated out on the surface with ethyl cellulose as a framework.

The novel fixing frame comprises a bottom plate and side plates, the side plates are arranged on the left side and the right side of the bottom plate in a welded mode, the side plates are provided with handrails and second door plates, the handrails are arranged above the second door plates, hollow grooves are formed between the second door plates and the handrails, and the second door plates are arranged in the hollow grooves. The side plates are provided with limiting mechanisms matched with the hollowed-out grooves. The limiting mechanism comprises a sliding rail, a sliding block and a steel pipe, the sliding block is arranged on the sliding rail in a sliding mode, a clamp is welded to the sliding block, and the steel pipe penetrates through the clamp and the hollowed-out groove. The novel fixing frame for artificial cultivation of calculus bovis is simple in structure, practical in function and ingenious in design, and effectively solves the problem that an existing fixing frame is not provided with a side face door plate for an operation, and inconvenience is brought to the operation process.

A method for screening and efficiently cultivating bezoar strains belongs to the field of bezoar strain screening, and comprises the following steps: step 1, inoculating a culture medium with low-pathogenicity Escherichia coli for culturing, comparing and observing, picking out single colonies with good growth vigor for subculture, and preserving the strains after culture; 2, taking the primarily screened preserved strain, reviving in four regions of an LB solid culture medium by marking lines, selecting a single colony, putting the single colony into a first gradient liquid bile culture medium, culturing, measuring OD600, and if OD600gt is detected, determining that the single colony is not detected; and 0.6, the strain can be placed in a liquid bile culture medium of the next gradient, and the strain which can grow in the three gradients can be inoculated into fresh oxgall for culture. The method has the advantages that the escherichia coli strain with high beta-glucuronidase activity for efficiently cultivating calculus bovis can be obtained, in-vitro cultivation verifies that the escherichia coli strain has bile resistance, calculus bovis analogues can be obtained, and the physicochemical properties of the calculus bovis analogues are similar to those of natural calculus bovis.

The disclosure relates to a traditional Chinese medicine composition, comprising active ingredients derived from Cornu caprae or Cornu saigae tataricae, Radix scutellariae, Bulbus fritillariae ussuriensis, Radix glycyrrhizae, Radix et rhizoma rhei, Gypsum fibrosum, Calculus bovis artifactus and Lapis Chloriti, the said active ingredients comprise in parts by weight: at least 1600 parts of amino acids; at least 120 parts of gallic acid; at least 130 parts of liquiritin; at least 20 parts of liquiritigenin; at least 400 parts of baicalin; at least 40 parts of oroxyloside; at least 120 parts of wogonoside; at least 280 parts of glycyrrhizic acid; at least 12 parts of chrysin-7-O-β-D-glucoronic acid; at least 50 parts of aloe-emodin-8-O-β-D-glucopyranoside; at least 30 parts of chrysophanol-1-O-β-D-glucopyranoside; at least 45 parts of chrysophanol-8-O-β-D-glucopyranoside; at least 260 parts of hyodeoxycholic acid; at least 150 parts of cholic acid. The traditional Chinese medicine composition in the present invention possess antipyretic, anti-inflammatory and antitussive effects.

The invention relates to a preparation method for producing calculus bovis by a chemical fermentation technology, which is characterized in that animal bile such as cattle (pigs, sheep and poultry) is used as a raw material, and the calculus bovis is produced by a modern process technology. The composition is prepared from the following components in parts by weight: 8 to 15 parts of taurine, 13 to 26 parts of ox gall powder, 1 to 12 parts of cholic acid, 1 to 17 parts of zinc sulfate, 1 to 15 parts of bilirubin, 20 to 40 parts of calcium gluconate, 20 to 38 parts of deoxycholic acid, 1 to 16 parts of cholesterol, 0.1 to 4.5 parts of fatty acid, 0.05 to 0.95 part of amylase and 0.05 to 2.22 parts of honeysuckle flower extract.