21 results about "Syringin" patented technology

The invention belongs to the technical field of traditional Chinese medicine detection, and particularly provides a construction method and a quality detection method of a specific chromatogram of a tinospora sinensis merr medicinal preparation, octadecylsilane chemically bonded silica is used as a filler, acetonitrile is used as a mobile phase A, an aqueous solution containing monopotassium phosphate is used as a mobile phase B, and a gradient elution procedure is continuously optimized; according to the method, the column temperature is controlled to be 23-27 DEG C, 11 common characteristic peaks are finally obtained, effective separation of 9 common characteristic peaks is achieved, the obtained characteristic chromatogram is stable in base line, good in characteristic peak shape and short in detection time, and a scientific basis is provided for comprehensively establishing the quality control standard of the tinospora sinensis merr formula granules. And moreover, the peak positions of tryptophan, syringin, isovanillin and magnoflorine can be accurately positioned, the known information of characteristic peaks is increased, and the integrity and characteristics of the tinospora sinensis merr medicinal preparations (such as preparations such as formula granules) are fully reflected.

The invention belongs to the technical field of traditional Chinese medicine detection, and particularly relates to a method for determining the content of multiple components in gout pills, which adopts high performance liquid chromatography to simultaneously determine the content of effective components such as aesculin, syringin, corilagin, ellagic acid and chebulilic acid in the gout pills, and adopts ultraviolet spectrophotometry to determine the content of total alkaloids in the gout pills. The method can effectively shorten the analysis time of main effective components in the gout pills, has a good linear range, is high in accuracy, precision and repeatability, is stable and feasible, and can be applied to content determination and quality standard of the gout pills.

The invention discloses a preparation process of an ovateleaf holly bark total saponin bulk drug, and particularly relates to the field of traditional Chinese medicine extraction and purification, the preparation process comprises the following steps: S1, extraction: crushing an ovateleaf holly bark medicinal material, and carrying out reflux extraction by using an ethanol aqueous solution as a solvent to obtain an ovateleaf holly bark crude extract; and S2, purification: carrying out solid-liquid separation on the crude ovateleaf holly bark extract, and purifying the filtrate through a recrystallization process and a macroporous resin adsorption process in sequence to obtain the ovateleaf holly bark total saponin bulk drug. According to the invention, an effective component group of ovateleaf holly bark for treating ulcerative colitis is taken as a target, the effective component group comprises syringin, pedunculoside and ziyuglycoside II, and a complete extraction-purification integrated process is constructed; through an orthogonal test, a comprehensive weighted scoring method is combined, and extraction parameters are scientifically optimized, so that the transfer rate of the three functional components is maximized, the extraction efficiency is high, and the pertinence is extremely high.

The application discloses a kind of Shenkun Yangxue granules fingerprint and its construction method, belong to traditional Chinese medicine detection technical field.Different batches of Shenkun Yangxue granules are prepared into test sample solution;Respectively with Danshensu, ephedrine hydrochloride, syringin, protocatechuic aldehyde, coffee acid, calycosin-7-O-β-D-glucoside, loganin, comphoric acid, shisonin, delphinidin and luteolin and kaempferol control preparation single control solution;Test sample solution and control solution are analyzed by high performance liquid chromatography and high resolution mass spectrometry respectively, 18 characteristic peaks are screened out, and 12 kinds of chemical components are identified by standard comparison and fragment information analysis, to obtain Shenkun Yangxue granules fingerprint.The Shenkun Yangxue granules fingerprint established by the method can be used for the quality control detection of Shenkun Yangxue granules, which is conducive to the comprehensive monitoring of product quality, and ensures the consistency, controllability and stability of product quality.

The invention relates to the technical field of drug detection, in particular to a fingerprint construction and multi-index component content determination method based on high performance liquid chromatography for a scutellaria baicalensis, manchurian lilac root and safflower cough-relieving preparation, which comprises the following steps: (1) preparation of a test solution: taking a sample of the scutellaria baicalensis, manchurian lilac root and safflower cough-relieving preparation, adding a methanol solution, performing ultrasonic treatment, and filtering to obtain the test solution; (2) preparation of a reference substance solution: dissolving a baicalin and / or syringin reference substance in a methanol solution to obtain the reference substance solution; (3) high performance liquid chromatography detection: injecting the test solution and the reference solution into a high performance liquid chromatograph, wherein the chromatographic conditions are as follows: a chromatographic column is an octadecylsilane chemically bonded silica packed column; the mobile phase A is methanol; the invention effectively solves the problem that a quantitative or qualitative detection method for the scutellaria baicalensis, manchurian lilac root and safflower cough-relieving mixture is lacked in the prior art, so that a method for constructing the fingerprint spectrum of the scutellaria baicalensis, manchurian lilac root and safflower cough-relieving preparation and determining the content of multiple index components based on high performance liquid chromatography is provided.

The invention provides a method for evaluating the anti-fatigue effect of acanthopanax senticosus, which comprises the following steps of: establishing an evaluation system taking the content of syringin and the IC50 value of DPPH free radical scavenging rate as double-effect indexes, and determining a clear judgment critical value of the anti-fatigue effect of acanthopanax senticosus tea: when and only when a sample simultaneously meets the conditions that the IC50 value of DPPH is less than or equal to 35mu g / mL and the content of syringin is more than or equal to 5mg / g, judging the anti-fatigue effect of acanthopanax senticosus tea; the obvious anti-fatigue effect is judged. Experiments prove that the double-index system and the threshold value have significant correlation with the anti-fatigue effect, the prediction deviation is small, the judgment is clear, the result is visual, and a standardized scheme is provided for rapid and low-cost effect evaluation of the acanthopanax tea product.

The present invention discloses a quality detection method for Nuan Gan Jian, belonging to the technical field of quality detection of traditional Chinese medicine. The method includes characteristic spectrum identification, and specifically comprises the following steps: (1) preparing a reference solution of syringin, a reference solution of norisoboldine, a reference solution of agarwood tetraol, a reference solution of cinnamaldehyde, a reference solution of cinnamic acid, and a reference solution of 6-gingerol; (2) preparing a test solution from Nuan Gan Jian granules; and (3) performing liquid chromatography detection on the six reference solutions and the test solution. The method has good comparability of detection results, reliable quality control, high detection efficiency, adapts to modern production needs, and realizes effective control of the quality of Nuan Gan Jian granules.

The present invention provides a method for simultaneously determining nine components in a safflower pharmaceutical composition, belonging to the field of drug detection technology. The method comprises preparing a reference solution I by taking uridine, guanosine, adenosine, p-hydroxybenzoic acid glycoside, 5-hydroxymethylfurfural, syringin, and p-hydroxysafflower yellow A; preparing a reference solution II by taking p-hydroxycinnamic acid and kaempferol 3-O-rutinoside; preparing a test solution by taking the safflower pharmaceutical composition; and performing high-performance liquid chromatography on the reference solution I, the reference solution II, and the test solution, respectively. The contents of the nine components in the safflower pharmaceutical composition are then calculated using an external standard method. The present invention utilizes a method for determining the contents of nine chemical components in a safflower pharmaceutical composition by high-performance liquid chromatography at one time, thereby achieving control of multiple index components in the safflower pharmaceutical composition.

The invention discloses a construction method of a fingerprint spectrum of Shenkun blood nourishing granules and the fingerprint spectrum of the Shenkun blood nourishing granules, and belongs to the technical field of traditional Chinese medicine detection. Different batches of Shenkun blood nourishing particles are adopted to prepare a test solution; the preparation method comprises the following steps: preparing a single reference substance solution from reference substances of tanshinol, leonurine hydrochloride, syringin, protocatechualdehyde, caffeic acid, calycosin-7-O-beta-D-glucoside, lobetyolin, formononin, rosmarinic acid, alkannic acid, luteolin and kaempferol; respectively performing high performance liquid chromatography and high resolution mass spectrometry on the test solution and the reference substance solution, totally screening 18 characteristic peaks, and totally identifying 12 chemical components through standard substance comparison and fragment information analysis to obtain the fingerprint spectrum of the Shenkun blood nourishing granules. The fingerprint spectrum of the Shenkun blood-nourishing granules established by the method can be used for quality control detection of the Shenkun blood-nourishing granules, is beneficial to comprehensive monitoring of product quality, and ensures consistency, controllability and stability of the product quality.

This invention discloses a cultivation substrate for promoting the growth of Acanthopanax senticosus seedlings and its application, belonging to the field of plant cultivation technology. The cultivation substrate for promoting the growth of Acanthopanax senticosus seedlings provided by this invention uses perlite and peat as a framework to construct a loose, breathable, water-retaining, and fertilizer-retaining physical structure. By adding Bacillus proteolyticus bacterial solution, its growth-promoting, antagonistic, and organic matter-decomposing effects are utilized to improve the rhizosphere microecology. Combined with controlled-release fertilizer, precise nutrient supply is achieved. These three elements synergistically optimize the water, fertilizer, air, and heat environment and microbial community in the rhizosphere of Acanthopanax senticosus seedlings, promoting nutrient absorption and metabolism, effectively improving photosynthetic efficiency and antioxidant enzyme (SOD, POD) activity; and significantly promoting the accumulation of medicinal components. The yield of eleutheroside E in the roots can reach 13.93 times that of the control group, and the yield of syringin in the leaves can reach 2279.82 μg, providing an efficient solution for cultivating high-quality, robust seedlings.

The invention provides an acanthopanax fruit and leaf composite tea bag and a preparation method thereof, treated deastringency fruits and high-aroma fried leaves are compounded according to the precise ratio of the fruits to the fried leaves being 1: (0.75-1.2), and syringin in the fruits and syringin, flavone and other components in the leaves generate the best synergistic interaction effect through precise compounding of the fruits and the leaves. Meanwhile, aiming at high astringent taste of the fruits, a low-temperature wall breaking process of less than or equal to 40 DEG C is adopted, and directional astringent taste removal is performed on the premise of retaining activity; and for the leaves, segmented temperature-controlled stir-frying (de-enzyming at 180 DEG C + slow baking at 120 DEG C) is adopted, so that the contradiction between aroma enhancement and glycoside retention is balanced. By improving the processing technology and optimizing the fruit and leaf compounding ratio, the product has the characteristics of reservation of high-activity components, high antioxidant activity and the like, has remarkable and predictable effects, has a solid material basis for health-care functions such as fatigue resistance and the like, and has extremely high market competitiveness.

The application belongs to the technical field of traditional Chinese medicine detection, and specifically provides a construction method and a quality detection method for characteristic chromatograms of Sarcandra glabra medicinal preparation, wherein octadecylsilane bonded silica gel is used as a filler, acetonitrile is used as mobile phase A, and a water solution containing potassium dihydrogen phosphate is used as mobile phase B, a gradient elution program is continuously optimized, the column temperature is controlled to be 23-27 DEG C, 11 common characteristic peaks are finally obtained, 9 common characteristic peaks are effectively separated, the characteristic chromatogram obtained has a smooth baseline, the characteristic peaks have good peak shapes, and the detection time is short, thereby providing a scientific basis for comprehensively establishing the quality control standard of Sarcandra glabra formula granules. Moreover, the peak positions of tryptophan, syringin, isovanillin and magnoflorine can be accurately positioned, the known information of the characteristic peaks is increased, and the integrity and characteristics of Sarcandra glabra medicinal preparation (for example, formula granules and other preparations) are fully reflected.

The application belongs to the technical field of natural medicine chemistry, and particularly relates to a method for extracting compounds in Magnolia officinalis by using a low eutectic solvent and a detection method. Magnolia officinalis powder and a low eutectic solvent are mixed, and then soaking and ultrasonic extraction are sequentially performed to obtain an extraction liquid containing target compounds; the target compounds are magnoliabine, syringin, magnolol and honokiol. The low eutectic solvent is used to assist ultrasonic extraction of the Magnolia officinalis powder, and strong hydrogen bond interaction is formed between the low eutectic solvent and the target compounds, which is beneficial to the extraction of the target compounds, so that the extraction rate of the four target compounds in the Magnolia officinalis powder is improved.

The invention belongs to the technical field of medicines, and particularly relates to syringin targeted inhibition DLAT protein and application of syringin targeted inhibition DLAT protein in inhibition of mycobacterium tuberculosis. Research finds that natural micromolecule syringin can inhibit DLAT protein activity in a targeted manner, reduce acetylation of MYC protein and promote ubiquitination and degradation of MYC protein, so that the MYC protein level is reduced, M2 type polarization of macrophages is inhibited, the polarization state of the macrophages is adjusted, growth of mycobacterium tuberculosis in the macrophages is inhibited, and the MYC-MYC-MYC-MYC-MYC-MYC-MYC-MYC-MYC-MYC is inhibited. The anti-tuberculosis effect is effectively achieved. Compared with an existing anti-tuberculosis technology, the anti-tuberculosis scheme is clear in action mechanism and accurate in targeting effect, and blindness of a traditional therapy is avoided. According to the present invention, the tuberculosis condition can be effectively controlled, the treatment period is expected to be shortened, the physical, mental and economic burden of the patient is reduced, the untoward effect risk is substantially reduced due to the high biological safety of the natural small molecules, and the new reliable path is provided for the clinical anti-tuberculosis treatment.

The invention discloses a preparation method and application of an acanthopanax senticosus extract for cosmetics, and relates to the technical field of acanthopanax senticosus extract.The acanthopanax senticosus extract is prepared from water, an acanthopanax senticosus composition, a preservative, a stabilizer, a thickening agent, an aromatic, a coloring agent and an auxiliary medicine mixture, through the synergistic effect of the optimized step-by-step composite solvent preparation-soaking process and the ultrasonic-assisted extraction technology, the dissolution rate and extraction efficiency of target active ingredients such as syringin are remarkably improved, and the content of the active ingredients in the obtained extract is greatly increased; by adding a stabilizer, a preservative and the like in the preparation process and adopting a specific step-by-step mixing and air-drying standing process, active ingredients are effectively protected, degradation is reduced, data related to preparation of the acanthopanax senticosus extract and corresponding analysis results are managed, visualized and stored, preparation management of the acanthopanax senticosus extract can be realized through internet cloud management and control, and the method is suitable for popularization and application of the acanthopanax senticosus extract. And the intelligent level of preparation management of the acanthopanax senticosus extract is improved.

The invention discloses a method for preparing a nano-emulsion by using an antioxidant surfactant and a product and application of the nano-emulsion, and belongs to the technical field of nano-emulsions.The nano-emulsion comprises 0.05 wt%-0.3 wt% of an active compound, 0.4 wt%-2.0 wt% of a fluorocarbon ascorbic acid derivative surfactant, 1.0 wt%-8.0 wt% of micromolecular polyol, 2.0 wt%-8.0 wt% of an oil solvent and the balance of water. The active compound comprises resveratrol or syringin, the fluorocarbon ascorbic acid derivative surfactant has oxidation resistance and water-oil amphipathy at the same time, the active compound is wrapped by the fluorocarbon ascorbic acid derivative surfactant, the composition types of raw materials are reduced, the stability of the active compound in a nano-emulsion system is improved, and the stability of the active compound in the nano-emulsion system is improved. And the prepared nano-emulsion has good dimensional stability.

The invention provides a compound processing technology of acanthopanax fruit leaves and a compound tea bag, according to the processing technology, acanthopanax fruits are processed by adopting a low-temperature wall breaking technology at the temperature of less than or equal to 40 DEG C, tannic acid in the fruits is physically and directionally degraded, and directional astringency removal and glycoside protection are realized; meanwhile, a segmented process of combining instant fixation at 180 DEG C with slow baking at 120 DEG C is designed for frying the leaves, so that the problem that substances such as syringin and total flavonoids in the leaves are easy to degrade is accurately solved, and efficient retention of core functional components is ensured; meanwhile, the synergistic increase of active ingredients is realized by optimizing the fruit and leaf compounding ratio. The system integration technology is adopted, the two independent process lines are integrated and optimized through sequential control and waste heat recovery, the technical contradiction that fruits and leaves cannot be produced on the same line is solved, and energy consumption and cost are greatly reduced.

This invention provides an LC-MS / MS method for the simultaneous detection of multiple major components of Shengmai Yin (Codonopsis pilosula formula) in plasma. The analytes include syringin, schisandrol A, gomisin D, schisandrol B, gomisin G, schisandrin A, schisandrin A, schisandrin B, codonopsis glycoside I, codonopsis alkynyl glycoside, methyl ophiopogon flavanone A, and methyl ophiopogon flavanone B. The method includes the following steps: preparation of a series of standard curve working solutions; preparation of standard curve samples; preparation of quality control working solutions; preparation of quality control samples; preparation of a mixed internal standard working solution; pretreatment of the standard curve, quality control, and analyte plasma samples using protein precipitation; sample detection; and quantitative determination based on the peak area ratio of the analyte to the internal standard to obtain the standard curve. The methodological investigation results of this invention show that the established method meets the requirements for in vivo biological sample determination, the pretreatment process is simple, and the method has good sensitivity, strong specificity, and high specificity, making it suitable for the detection of compounds with low concentrations.

The invention provides a method for determining the content of multiple components in an Aidi preparation, and belongs to the technical field of medicines. The Aidi preparation is prepared from traditional Chinese medicinal materials including ginseng, cantharides, astragalus membranaceus and acanthopanax. The content determination method comprises the following steps: simultaneously determining cantharidin, ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1, ginsenoside Rc, ginsenoside Rd, isofraxidin, syringin, formononetin, astragaloside, chlorogenic acid, eleutheroside E, calycosin-7-glucoside, astragaloside II, neochlorogenic acid, cryptochlorogenic acid and eleutheroside E1 in the Aidi preparation by adopting liquid chromatography-mass spectrometry; the composition is prepared from effective components such as ginsenoside F3, ginsenoside Rg2, azelaic acid and the like. Compared with the prior art, the method has the advantages that the contents of the 20 effective components in the Aidi preparation are simultaneously measured under the same chromatographic condition through liquid chromatography-mass spectrometry, the method is convenient and rapid, the requirement for product quality detection is greatly improved, the method is more suitable for quality control of drugs in the future, and the drug quality and the clinical drug effect are guaranteed.

The invention discloses a quality detection method of a liver-warming decoction, and belongs to the technical field of quality detection of traditional Chinese medicines. The method comprises specific chromatogram identification and comprises the following specific steps: (1) respectively preparing a syringin reference substance solution, a norisoboldine reference substance solution, an agilawood tetrol reference substance solution, a cinnamaldehyde reference substance solution, a cinnamic acid reference substance solution and a 6-gingerol reference substance solution; (2) preparing a test solution from the warming liver decoction granules; and (3) respectively carrying out liquid chromatography detection on the six reference substance solutions and the test solution. The method is good in detection result comparability, reliable in quality control and high in detection efficiency, meets modern production requirements, and realizes effective control of the quality of the liver-warming decoction granules.

The invention belongs to the technical field of under-forest planting, and particularly relates to an ecological cultivation method for intercropping cortex acanthopanacis under a Chinese fir forest, and the method comprises the steps: collecting under-forest long-term ecological factors and growth historical data, constructing an improved GWO optimized LSTM prediction model, predicting the ecological factors and cortex acanthopanacis growth indexes in the future 7 days, comparing the most suitable ecological factor range, and determining the growth index of cortex acanthopanacis. When the difference value exceeds the limit, the under-forest ecological environment is regulated and controlled in real time through cultivation measures such as optimizing the opening degree of the sunshade net through WOA. According to the cultivation method, the growth indexes such as the diameter at breast height and the height of the cedarwood and the root thickness, the root bark thickness and the syringin content of the cortex acanthopanacis can be remarkably improved, the yield of the cortex acanthopanacis is increased, meanwhile, the land resource utilization rate and the economic benefit per unit area are increased, and an effective technical mode can be provided for under-forest economic development.