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Acanthopanax senticosus detection method

A detection method, the technology of Acanthopanax, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., to achieve the effects of short detection time, stable baseline and accurate detection results

Inactive Publication Date: 2016-07-27
CHENGDU KANGHONG PHARMA GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problem of quality control of Acanthopanax senticosus, the present invention provides a simple, rapid, stable baseline, and good separation of Acanthopanax senticosus detection method

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The detection method of comparative example one Acanthopanax

[0036] The preparation method of the test solution: get about 2 g of Acanthopanax superficialis coarse powder, accurately weighed, put in a stoppered Erlenmeyer flask, accurately add 25 ml of methanol, weigh, ultrasonically treat (250w, frequency 40khz) for 30 minutes, let cool , and then weigh the weight, make up the lost weight with methanol, shake well, filter, and take the filtrate, that is.

[0037] Chromatographic conditions: Octadecyl bonded silica gel (VP-ODS4.6mm×250mm, 5μm), mobile phase is acetonitrile-water (20:80), flow rate 1mL / min, column temperature is room temperature, detection wavelength 265nm.

[0038] Accurately draw respectively 10 μ l of the reference substance solution and the test solution and inject it into the liquid chromatograph, and carry out liquid chromatographic detection. figure 1 It is the detection result of syringin, the baseline of the chromatogram is seriously unstabl...

Embodiment 2

[0039] The detection method of comparative example Er Acanthopanax

[0040] Sample preparation method: take about 2g of Acanthopanax quincis powder, accurately weigh it, put it in a stoppered conical flask, add 25ml of methanol precisely, weigh it, ultrasonically treat it (250w, frequency 40khz) for 30 minutes, let it cool, and weigh again Determine the weight, make up the lost weight with methanol, shake well, filter, and take the filtrate to obtain the final product.

[0041] Chromatographic conditions: Octadecyl bonded silica gel as filler (AglientPoroshellSB-C182.7μm2.1×100mm), mobile phase is acetonitrile-water (20:80), flow rate 0.2mL / min, column temperature is room temperature, detection The wavelength is 265nm.

[0042] Accurately draw respectively 2 μ l of the reference substance solution and the test solution and inject it into the liquid chromatograph, and carry out liquid chromatographic detection. figure 2 It is the detection result of syringin. The resolution ...

Embodiment 3

[0052] The detection method of embodiment three Acanthopanax

[0053] Preparation of Reference Substance Solution Take an appropriate amount of syringin reference substance, accurately weigh it, add methanol to make a solution containing 80 μg per 1 ml, and obtain it.

[0054] Preparation of the test solution Take about 2 g of the coarse powder of this product, accurately weigh it, put it in a stoppered Erlenmeyer flask, add 25 ml of methanol precisely, weigh it, ultrasonically treat it (250W, frequency 40khz) for 30 minutes, let it cool, and then Weigh the weight, make up the lost weight with methanol, shake well, filter, and take the filtrate to obtain the final product.

[0055] Chromatographic conditions Use octadecylsilane bonded silica gel as filler (aglientSB-C18 2.7μm2.1×100mm); use acetonitrile-water (8:92) as mobile phase; flow rate 0.5ml / min; column temperature 40°C; The detection wavelength is 265nm.

[0056] Determination method Precisely draw 2 μl each of the r...

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Abstract

The invention provides an acanthopanax senticosus detection method; a liquid chromatography method is used for detecting a test sample solution and a reference solution, and liquid chromatography determination conditions comprise that octadecylsilane bonded silica gel is used as a filler; an acetonitrile-water mixture is used as a mobile phase, and an external standard method is used for detecting the content of syringin in a test sample. The detection method has the advantages of smooth baseline and good separating degree, can effectively detect and quantify the effective compound syringin in acanthopanax senticosus.

Description

technical field [0001] The invention relates to the field of detection of medicinal materials, in particular to a detection method of Acanthopanax. Background technique [0002] Acanthopanax, found in "Shen Nong's Materia Medica", is listed as top grade. Top grade refers to non-toxic, long-term use can lighten the body, prolong life without harm. Acanthopanax has been regarded as a good medicine with essence-replenishing and anti-aging effects since ancient times. It was originally produced in the mountainous area of ​​Heilongjiang Province. The former Soviet Union is also known as Siberian ginseng. Li Shizhen's "Compendium of Materia Medica" in the Ming Dynasty called Ciwujia, and the five leaves are good, so it is called Wujia, also known as Wuhua. Wujia treats rheumatism, strengthens the muscles and bones, and its merits are profound. It is better to have a handful of Wujia than to fill the car with gold and jade. Also, the saying that "articles can be used to make win...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 柯潇白晓春邬智刚
Owner CHENGDU KANGHONG PHARMA GRP
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