Nine-element wind-extinguishing particle component quantitative detection method and fingerprint construction method

A quantitative detection method, a technology of nine flavors to extinguish the wind, applied in the field of analytical chemistry, to achieve the effects of good linear relationship, accurate detection, and stable baseline

Active Publication Date: 2016-02-03
JIANGSU KANION PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no clear report on the quality control method of Jiuwei Xifeng Granules, so it is of great practical significance to provide a quality control method for Jiuwei Xifeng Granules

Method used

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  • Nine-element wind-extinguishing particle component quantitative detection method and fingerprint construction method
  • Nine-element wind-extinguishing particle component quantitative detection method and fingerprint construction method
  • Nine-element wind-extinguishing particle component quantitative detection method and fingerprint construction method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1 9

[0074] Example 1 Quantitative detection method for components of Jiuwei Xifeng granules

[0075] 1. Chromatographic conditions and system adaptability test: octadecylsilane bonded silica gel is used as filler (chromatographic column: PhenomenexGeminiC 18 , The column length is 250mm, the inner diameter is 4.6mm, and the particle size is 5μm); methanol is used as mobile phase A, and 0.1% triethylamine is used as mobile phase B. Gradient elution is performed as specified in the following table; flow rate is 1.0mL / min; The detection wavelength is 246nm; the column temperature is 35℃; the number of theoretical plates is not less than 5000 calculated by the rhynchophylline peak.

[0076] Table 1 Gradient elution program

[0077] Time (minutes)

Mobile phase A (%)

Mobile phase B (%)

0~5

40

60

5~30

40→60

60→40

30~50

60→75

40→25

50~60

75

25

[0078] 2. Preparation of reference substance solution: accurately weigh the reference substance rhynchophylline, isorynchophylline, ...

Embodiment 2 9

[0082] Example 2 Quantitative detection method for components of Jiuwei Xifeng granules

[0083] 1. Chromatographic conditions and system adaptability test: use octadecylsilane bonded silica as filler (chromatographic column: Thermosyncronis C18 chromatographic column, column length is 250mm, inner diameter is 4.6mm, particle size is 5μm); using methanol as flow Phase A, with 0.05% triethylamine as mobile phase B, perform gradient elution as specified in Table 1 of Example 1; Flow rate is 1.2mL / min; Detection wavelength is 230nm; Column temperature is 30℃; The number of theoretical plates is as per hook The calculation of the caneline peak is not less than 5000.

[0084] 2. Preparation of reference substance solution: accurately weigh the reference substance rhynchophylline, isorynchophylline, rhynchophylline, and rhynchophylline, and add 80% ethanol to make rhynchophylline 45.05μg / mL , Isorhynchophylline 45.06μg / mL, Dehydrorhynchophylline 50.18μg / mL, Isorhynchophylline 50.29μg / mL...

Embodiment 3 9

[0087] Example 3 Quantitative detection method for components of Jiuwei Xifeng granules

[0088] 1. Chromatographic conditions and system adaptability test: use octadecylsilane bonded silica as filler (chromatographic column: Agilent5TC-C18 chromatographic column, column length is 250mm, inner diameter is 4.6mm, particle size is 5μm); with methanol As mobile phase A, with 0.15% triethylamine as mobile phase B, perform gradient elution as specified in Table 1 of Example 1; flow rate is 0.8mL / min; detection wavelength is 260nm; column temperature is 40°C; number of theoretical plates Calculated according to the rhynchophylline peak, not less than 5000.

[0089] 2. Preparation of reference substance solution: accurately weigh the reference substance rhynchophylline, isorynchophylline, rhynchophylline, and rhynchophylline, and add 80% ethanol to make rhynchophylline 45.05μg / mL , Isorhynchophylline 45.06μg / mL, Dehydrorhynchophylline 50.18μg / mL, Isorhynchophylline 50.29μg / mL as a mixed ...

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Abstract

The present invention relates to the technical field of analytical chemistry, and particularly relates to a nine-element wind-extinguishing particle component quantitative detection method and a fingerprint construction method. The detection method comprises taking a nine-element wind-extinguishing particle to-be-tested sample and a standard substance for HPLC detection, and obtaining nine-element wind-extinguishing particle components and contents according to HPLC detection results; wherein HPLC detection chromatographic conditions are as follows: a C18 chromatographic column is used, methanol is used as a mobile phase A, and triethylamine is used as a mobile phase B for gradient elution. The fingerprint measured by the detection method can more fully reflect nine-element wind-extinguishing particle chemical components, chromatographic peak separation is good, baseline is steady, peak forms are good, reproducibility is good, the method has good linear relationship, precision, stability and recovery rate, and can accurately detect the nine-element wind-extinguishing particle chemical component content, and the obtained control fingerprint can objectively assess the quality of the sample.

Description

Technical field [0001] The invention relates to the technical field of analytical chemistry, in particular to a method for quantitatively detecting the components of Jiuweixifeng particles and a method for constructing a fingerprint spectrum. Background technique [0002] Tourette syndrome, also known as Tourette syndrome, is a hereditary neuropsychiatric disease characterized by chronic, fluctuating, and multiple motor tics, accompanied by involuntary vocalization. Tic multiple has become more common, and many children come to see a doctor every year in primary hospitals alone. In recent years, the incidence of multiple tics has increased significantly. Epidemiological investigations have shown that the annual incidence of tics is generally 0.5 to 1 / 100,000 (the number of new patients per 100,000 each year). Tic multiple disease is significantly more common in men than in women, and the results reported in the literature are quite different. The ratio of men to women is from 1....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 萧伟付娟李家春黄文哲王振中周习
Owner JIANGSU KANION PHARMA CO LTD
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