15results about How to "Good peak shape" patented technology

The application discloses a kind of radionuclide 18 The present application discloses a method for detecting the radiochemical purity of F-labeled FAPI compounds, which comprises using thin layer chromatography to detect the radiochemical purity of F-labeled FAPI compounds 18 The present application discloses a method for detecting the radiochemical purity of F-labeled FAPI compounds, which comprises using thin layer chromatography to detect the radiochemical purity of F-labeled FAPI compounds 18 The present application discloses a method for detecting the radiochemical purity of F-labeled FAPI compounds, which comprises using thin layer chromatography to detect the radiochemical purity of F-labeled FAPI compounds 18 The present application discloses a method for detecting the radiochemical purity of F-labeled FAPI compounds, which comprises using thin layer chromatography to detect the radiochemical purity of F-labeled FAPI compounds 18 The present application discloses a method for detecting the radiochemical purity of F-labeled FAPI compounds, which comprises using thin layer chromatography to detect the radiochemical purity of F-labeled FAPI compounds 18 The present application discloses a method for detecting the radiochemical purity of F-labeled FAPI compounds, which comprises using thin layer chromatography to detect the radiochemical purity of F-labeled FAPI compounds 18 The present application discloses a method for detecting the radiochemical purity of F-labeled FAPI compounds, which comprises using thin layer chromatography to detect the radiochemical purity of F-labeled FAPI compounds 18 The present application discloses a method for detecting the radiochemical purity of F-labeled FAPI compounds, which comprises using thin layer chromatography to detect the radiochemical purity of F-labeled FAPI compounds 18 The present application discloses a method for detecting the radiochemical purity of F-labeled FAPI compounds, which comprises using thin layer chromatography to detect the radiochemical purity of F-labeled FAPI compounds 18 The present application discloses a method for detecting the radiochemical purity of F-labeled FAPI compounds, which comprises using thin layer chromatography to detect the radiochemical purity of F-labeled FAPI compounds 18 The present application discloses a method for detecting the radiochemical purity of F-labeled FAPI compounds, which comprises using thin layer chromatography to detect the radiochemical purity of F-labeled FAPI compounds

The invention discloses a Sorosil decoction specific chromatogram and a construction method and application thereof, and belongs to the technical field of traditional Chinese medicine analysis and detection. The method comprises the following steps: respectively dissolving reference substances of bergenin, arbutin, glycyrrhizic acid, liquiritin, liquiritigenin and gallic acid in a solvent to obtain reference substance solutions; adding soroxil decoction into a solvent, carrying out ultrasonic extraction, and filtering to obtain a test solution; and injecting the reference solution and the test solution into a high performance liquid chromatograph for detection, taking the specific chromatogram of the reference solution as a reference chromatogram, selecting common peaks from the specific chromatogram of the test solution, and constructing the specific chromatogram of the soroxil decoction. The invention also discloses a Sorosil decoction characteristic spectrum and application thereof. According to the specific chromatogram of the soroxi decoction, rapid identification of the formula of the soroxi decoction can be realized, the method is simple, the research blank of the specific chromatogram in quality control of the classic famous-prescription soroxi decoction and the preparation thereof is filled up, and a powerful guarantee is provided for quality improvement of the classic famous-prescription soroxi decoction.

The invention belongs to the technical field of pesticide residue detection, and relates to a method for detecting residues of four herbicides in farmland soil through QuEChERS pretreatment-high performance liquid chromatography-tandem mass spectrometry. The method comprises the following specific steps: (1) drawing a standard curve: preparing a mixed standard solution of four pesticides including topramezone, bicyclopyrone, isoxaflutole and dicarfentrazone, carrying out high performance liquid chromatography-tandem mass spectrometry analysis, and drawing the standard curve according to the concentrations of the four pesticide standard solutions and quantitative ion pair peak areas; (2) QuEChERS pretreatment: performing solvent extraction, salt extraction and purifying agent treatment on to-be-detected soil to obtain a to-be-detected sample solution; and (3) analyzing the concentration of the pesticide residues: performing high performance liquid chromatography-tandem mass spectrometry analysis on the sample solution to be detected, and calculating to obtain the concentration of the four pesticide residues in the soil to be detected. The method is simple in pretreatment, high in detection recovery rate, good in detection result repeatability and high in detection sensitivity.

The application provides a kind of Fuling drink piece, fried Fuling drink piece and the construction method and application of its preparation characteristic map, the construction method of fried Fuling and its preparation characteristic map, comprising: preparing test sample solution;Using high performance liquid chromatography to detect test sample solution, chromatographic conditions include with octadecylsilane bonded silica as filler, with methanol as mobile phase A, with 0.08%-0.4% formic acid as mobile phase B, and using specific elution procedure, while shortening the detection time also significantly improves the separation effect of multiple effective components, so that the characteristic map contains more characteristic peaks, greatly enriches the atlas information, realizes the effective separation of characteristic peaks such as uridine, thymine, gallic acid and guanosine, the characteristic of the obtained characteristic map is obvious, fully reflects the integrity and characteristic of the drug preparation of fried Fuling, and the baseline is stable, the peak shape is good, which provides basis for comprehensively detecting the quality of Fuling drink piece, fried Fuling and its pharmaceutical preparations.

The invention provides a construction method and a quality detection method of a characteristic spectrum of a traditional Chinese medicine composition, and the construction method of the characteristic spectrum comprises the following steps: taking a solution of a traditional Chinese medicine composition test sample and a solution of a reference substance, and carrying out high performance liquid chromatography detection to obtain the characteristic spectrum of the traditional Chinese medicine composition; wherein the traditional Chinese medicine composition comprises radix scutellariae, cortex mori radicis, fructus trichosanthis, semen armeniacae amarae, radix adenophorae, radix ophiopogonis, semen coicis, semen benincasae, fructus aurantii, radix curcumae, rhizoma imperatae, radix salviae miltiorrhizae, indigo naturalis and clam shells; the liquid chromatographic analysis conditions of the detection are as follows: octadecylsilane chemically bonded silica is used as a filler, acetonitrile is used as a mobile phase A, a phosphoric acid solution with the volume percentage of 0.09-0.11% is used as a mobile phase B, gradient elution is performed, the detection wavelength is 207-286nm, the column temperature is 25-35 DEG C, and the flow velocity is 0.9-1.1 mL / min. The specific chromatogram constructed by the invention can objectively evaluate the quality of a traditional Chinese medicine composition sample and improve the quality of the traditional Chinese medicine composition, so that the curative effect is ensured.

The application discloses a kind of shentong zhuyu decoction fingerprint construction method and application, belong to the technical field of drug detection.The application is detected by high performance liquid chromatography to several batches of shentong zhuyu decoction by the selection of mobile phase and the cooperation of chromatographic column, the high performance liquid chromatography of different batches of shentong zhuyu decoction is compared, obtains the common peak containing loganic acid, amygdalin, hydroxyl safflower yellow pigment A, aniseed acid, qianghuo alcohol, isoeucommarin, ammonium glycyrrhizinate, kaempferol characteristic peak, and the fingerprint of shentong zhuyu decoction is constructed according to the common peak.The fingerprint constructed by the application can better detect 12 medicinal materials, shorten the detection time of single sample to 125 min or less, improve efficiency, and improve the quality control level of product.

This application discloses a method for detecting astaxanthin content. The provided method includes the following steps: mixing the sample to be tested with acetone to prepare a test solution; performing high-performance liquid chromatography (HPLC) on the test solution. The HPLC conditions include: using tert-butyl methyl ether as mobile phase A and a mixed solution of methanol and acetonitrile as mobile phase B, with isocratic elution. The provided detection method uses HPLC, employing a mixed solvent of tert-butyl methyl ether and methanol-acetonitrile as the mobile phase, and successfully achieves efficient and rapid separation of astaxanthin through isocratic elution. The chromatographic peak shape is excellent, effectively overcoming interference from proteins, lipids, nutrients, and ionic components in a complex matrix, providing a reliable analytical tool for assessing the intestinal absorption characteristics of astaxanthin.

The present application relates to the technical field of traditional Chinese medicine detection, and specifically provides a characteristic spectrum construction method of Herba Centipedae and its preparation. A specific elution procedure is obtained through a large number of experiments by using octadecylsilane bonded silica gel as a filler and a mobile phase including methanol and an acid-containing aqueous solution for gradient elution, 13 or more common characteristic peaks are obtained, and effective separation of characteristic peaks including neochlorogenic acid, caffeic acid, chlorogenic acid, cryptochlorogenic acid, 1,4-dicaffeoylquinic acid, 1,5-dicaffeoylquinic acid, big pothos glycoside and thymotin lactone ketone is achieved, the peak shape is good, the baseline is smooth, the determined components can be completely separated, the detection time is short, and the method provides a basis for quality detection and control of Herba Centipedae and its preparation, can fully reflect the integrity and characteristics of Herba Centipedae and its preparation, and is simple to operate and applicable to quality control of different preparation types of Herba Centipedae.

This invention belongs to the field of pharmaceutical analysis technology, and specifically relates to a method for determining the content of catechins in a standard decoction of kiwifruit root and a method for constructing a characteristic chromatogram. High-performance liquid chromatography (HPLC) is used for determination. The HPLC conditions include: an octadecylsilane-bonded silica column as the packing material; methanol as mobile phase A; and a 0.05–0.2% (v / v) formic acid aqueous solution as mobile phase B, with gradient elution. This method has strong specificity, good repeatability and robustness, and high stability and accuracy. The constructed characteristic chromatogram and detection method can rapidly and comprehensively achieve the joint control of multiple components in the standard decoction of kiwifruit root, realizing quality control of kiwifruit root from raw material to preparation, which is beneficial to ensuring the effectiveness of clinical medication.

This invention relates to the field of traditional Chinese medicine component analysis technology, specifically to the fingerprint spectrum of Juanxiao tablets and its establishment method. The method for establishing the fingerprint spectrum of Juanxiao tablets includes: using isoharonidin, isovitexin, rutin, hesperidin, lemon balm glycoside, aloe-emodin, nobiletin, and citrus reticulataein as reference standards, and Juanxiao tablets as the test sample; using HPLC to detect the reference standards and the test sample, and then comparing and analyzing them to determine the common characteristic peaks of Juanxiao tablets; wherein the HPLC conditions include: a C18 column, mobile phase A as a nitrile solvent, mobile phase B as a 0.05-0.15% phosphoric acid solution, and gradient elution. This method can effectively detect characteristic peaks, reduce the drying of impurity peaks, and the obtained fingerprint spectrum has high resolution and good peak shape. All characteristic peaks are baseline separated, enabling a more comprehensive and accurate evaluation of the quality of Juanxiao tablets.

The invention provides a method for detecting volatile and semi-volatile organic compounds in aerosol of a heated cigarette and application of the method. The method for detecting the volatile and semi-volatile organic compounds in the aerosol of the heated cigarette comprises the following steps: (1) smoking the heated cigarette to be detected, and trapping the aerosol by using a filter disc and an adsorbent; (2) mixing the filter disc capturing the aerosol, the adsorbent, an internal standard and a solvent, and extracting to obtain an extract; and (3) carrying out gas chromatography-tandem mass spectrometry detection on the extract, and obtaining the content of volatile and semi-volatile organic compounds in the heated cigarette to be detected according to the detection result. The method provided by the invention can simultaneously separate and analyze the content of volatile and semi-volatile organic compounds in various aerosols, improves the detection flux, improves the analysis efficiency, simplifies the pretreatment process, has the advantages of high flux, good peak shape, high separation degree, high sensitivity, good recovery rate and the like, and is suitable for industrial production. The method is suitable for analysis and determination of main volatile and semi-volatile organic compounds in heated cigarette aerosol.

The utility model discloses an integrated full -automatic headspace sample injector and gas chromatograph combined device, including headspace sample injector, valve box, transmission pipe and gas chromatography unit, the valve box is located in the high temperature area of gas chromatography unit one end sample injection port vicinity, the sample injection port of gas chromatography unit is connected with valve box through transmission pipe, the utility model discloses a headspace sample injector valve box split design, and the headspace sample injector valve box is placed in the high temperature area of the sample injection port vicinity of the matched gas chromatography unit, and the valve box independent heating temperature control is favorable to the rapid heating and heat preservation of valve box, is favorable to the saving of electric energy, shortens the transmission pipe length between headspace sample injector and the sample injection port of gas chromatography unit, improves chromatographic peak shape, and improves analysis result accuracy, and transmission pipe is small and subtle, and the appearance of headspace sample injector and gas chromatograph matched combination is harmonious and beautiful.

The utility model discloses a connecting device for improving detection sensitivity based on ion chromatography transformation, which comprises a chromatographic column, a column oven, a suppressor and a detector, the chromatographic column is arranged in the column oven; the suppressor is provided with a leacheate inlet, and the leacheate inlet is connected with the outlet of the chromatographic column; the suppressor is provided with a leacheate outlet, and the leacheate outlet is connected with an inlet of the detector; the suppressor is provided with a regenerated liquid inlet, and the regenerated liquid inlet is connected with an outlet of the detector; and the suppressor is powered by a direct-current stabilized power supply. According to the device, the column oven and the suppressor are additionally arranged, compared with original old equipment, the suppressor improves sensitivity, the column oven optimizes the separation degree, and efficient analysis of complex samples can be achieved through combination of the suppressor and the column oven.

This invention provides an LC-MS / MS method for the simultaneous detection of multiple major components of Shengmai Yin (Codonopsis pilosula formula) in plasma. The analytes include syringin, schisandrol A, gomisin D, schisandrol B, gomisin G, schisandrin A, schisandrin A, schisandrin B, codonopsis glycoside I, codonopsis alkynyl glycoside, methyl ophiopogon flavanone A, and methyl ophiopogon flavanone B. The method includes the following steps: preparation of a series of standard curve working solutions; preparation of standard curve samples; preparation of quality control working solutions; preparation of quality control samples; preparation of a mixed internal standard working solution; pretreatment of the standard curve, quality control, and analyte plasma samples using protein precipitation; sample detection; and quantitative determination based on the peak area ratio of the analyte to the internal standard to obtain the standard curve. The methodological investigation results of this invention show that the established method meets the requirements for in vivo biological sample determination, the pretreatment process is simple, and the method has good sensitivity, strong specificity, and high specificity, making it suitable for the detection of compounds with low concentrations.

This invention discloses an ultra-high performance liquid chromatography (UHPLC) method for the simultaneous determination of multiple kavalactone components in kava extract. The method includes: S1, preparing a ternary mobile phase system using 0.1% phosphoric acid aqueous solution as mobile phase A, acetonitrile as mobile phase B, and isopropanol as mobile phase C; S2, preparing a mixed reference solution containing capsaicin, demethoxycapsaicin, dihydrocapsaicin, capsaicin, capsaicin, dihydrocapsaicin, yellow kavalactin A, yellow kavalactin B, and yellow kavalactin C; S3, preparing a test solution; and S4, performing gradient elution determination using an UHPLC system with octadecylsilane-bonded silica gel as the packing material. This invention fills the monitoring gap for yellow kavalactin C; all components achieve baseline separation, exhibiting advantages such as good separation, short analysis time, high precision, and good stability, providing a comprehensive and reliable detection method for the quality control of kava extract.