52results about How to "Good peak shape" patented technology

The invention provides a construction method and application of an angelica sinensis potion fingerprint spectrum, and belongs to the technical field of drug detection.The construction method includes the steps that firstly, an angelica sinensis potion is used for preparing a test solution, then 0.08-0.12 wt% phosphoric acid aqueous solution (A)-acetonitrile (B) serves as a mobile phase, and the angelica sinensis potion fingerprint spectrum is obtained through high performance liquid chromatography detection, a standard fingerprint spectrum is established and is used for quality evaluation or control in the whole process of research / development / production / clinical application of the angelica sinensis potion. According to the construction method of the fingerprint spectrum of the angelica sinensis potion, main medicinal material components in the angelica sinensis potion can be detected only through high performance liquid chromatography, so that the quality condition of the angelica sinensis potion is reflected more comprehensively.

The invention discloses a construction method and application of an HPLC (High Performance Liquid Chromatography) specific chromatogram of sunburn prevention and treatment cream, and relates to the technical field of pharmaceutical analysis. The sunshine prevention and treatment cream is composed of honeysuckle, perfoliote knotweed herb, sedum sarmentosum, dayflower, radix polygonati officinalis, lithospermum erythrorhizon, aloe, asparagus fern, lucid ganoderma, coix seed and bee pollen, and is one of representative products of Guizhou Miao medicine brands. The specific chromatogram of the sunshine prevention and treatment cream is established by adopting a high performance liquid chromatography, and the five medicinal materials including honeysuckle, lithospermum erythrorhizon, aloe, sedum sarmentosum and perfoliote knotweed herb can be identified at the same time. The HPLC characteristic chromatogram of the sunshine prevention and treatment cream established by the method can effectively characterize the quality of the sunshine prevention and treatment cream, and is beneficial to comprehensively monitoring the quality of medicinal materials. Meanwhile, the method has the advantages of being simple, convenient, stable, high in precision, good in reproducibility and the like, and the authenticity of the product can be rapidly and accurately identified.

The invention relates to the technical field of glucose detection, and discloses a method for detecting glucose in d-ribose fermentation broth, which comprises the following steps: step 1, diluting a d-ribose fermentation broth sample and adjusting the d-ribose fermentation broth sample to a predetermined pH value to form a pretreated sample; 2, removing impurities and concentrating target components through solid-phase extraction to obtain a treated sample; 3, separating the sample by using high performance liquid chromatography, setting a mobile phase, a flow rate and a chromatographic column temperature, and separating to obtain a glucose component; and 4, injecting the separated components into a mass spectrum system on line, and detecting a characteristic ion signal of glucose. The high-sensitivity and accurate detection of glucose in the d-ribose fermentation liquor can be realized by adopting a high-performance liquid chromatography and mass spectrometry combined technology. Through optimized sample pretreatment and mobile phase selection, interference of other carbohydrates on glucose detection is reduced, and accurate determination of glucose concentration is ensured.

The present invention provides a detection method and application of related substances in cationic lipid YK-009, the detection method comprising the following steps: (1) mixing a sample to be tested with a diluent to obtain a test solution; (2) mixing a related substance standard with a diluent to obtain a reference solution; (3) subjecting the test solution and the reference solution to liquid chromatography detection, and calculating the content of the related substances according to the detection results; the mobile phase of the liquid chromatography detection comprises a mobile phase A and a mobile phase B, the mobile phase A is an aqueous solution of acetic acid-triethylamine, and the mobile phase B is an acetonitrile solution of acetic acid-triethylamine. The detection method provided by the present invention can accurately detect a variety of related substances in YK-009 at the same time, has high sensitivity, high accuracy, high reproducibility, good durability, and is convenient for accurate detection and quality control of related substances in YK-009.

The invention relates to the technical field of medicine detection, in particular to a method for detecting impurities of dextroborneol. The detection method provided by the invention comprises the following steps: providing a dextrocamphol test solution and a reference substance solution of dextrocamphol impurities, wherein the dextrocamphol impurities comprise at least one of dextrocamphol, isoborneol and L-borneol; carrying out gas chromatography-mass spectrometry detection on the dextrocamphol test solution and the reference substance solution of the dextrocamphol impurity; wherein certain chromatographic conditions and mass spectrum conditions are set. According to the embodiment of the invention, by setting related chromatographic conditions and mass spectrum conditions, the final detection result shows that the peak pattern is good, the separation degree is excellent, the method has obvious advantages in specificity, quantitation limit, detection limit, linearity, precision, accuracy and durability, and a detection thought is provided for impurity detection of dextroborneol.

The invention belongs to the technical field of quality detection of traditional Chinese medicines, and particularly relates to an HPLC (high performance liquid chromatography) fingerprint spectrum detection method of a Qingyanli tablet, which comprises the step of detecting the Qingyanli tablet by adopting a high performance liquid chromatography, and the chromatographic conditions are as follows: an acetic acid aqueous solution with the mass concentration of 1% is used as a mobile phase A, acetonitrile is used as a mobile phase B, gradient elution is performed, and the HPLC fingerprint spectrum detection method is used for detecting the Qingyanli tablet. According to the method disclosed by the invention, each chromatographic peak is well separated, the characteristic peaks are more, the peak shape is good, the separation degree is better, the detection can be more accurate, the method has better detection stability, consistency and controllability, and the quality of the throat clearing tablets can be more comprehensively and reliably detected.

The application discloses a kind of radionuclide 18 The present application discloses a method for detecting the radiochemical purity of F-labeled FAPI compounds, which comprises using thin layer chromatography to detect the radiochemical purity of F-labeled FAPI compounds 18 The present application discloses a method for detecting the radiochemical purity of F-labeled FAPI compounds, which comprises using thin layer chromatography to detect the radiochemical purity of F-labeled FAPI compounds 18 The present application discloses a method for detecting the radiochemical purity of F-labeled FAPI compounds, which comprises using thin layer chromatography to detect the radiochemical purity of F-labeled FAPI compounds 18 The present application discloses a method for detecting the radiochemical purity of F-labeled FAPI compounds, which comprises using thin layer chromatography to detect the radiochemical purity of F-labeled FAPI compounds 18 The present application discloses a method for detecting the radiochemical purity of F-labeled FAPI compounds, which comprises using thin layer chromatography to detect the radiochemical purity of F-labeled FAPI compounds 18 The present application discloses a method for detecting the radiochemical purity of F-labeled FAPI compounds, which comprises using thin layer chromatography to detect the radiochemical purity of F-labeled FAPI compounds 18 The present application discloses a method for detecting the radiochemical purity of F-labeled FAPI compounds, which comprises using thin layer chromatography to detect the radiochemical purity of F-labeled FAPI compounds 18 The present application discloses a method for detecting the radiochemical purity of F-labeled FAPI compounds, which comprises using thin layer chromatography to detect the radiochemical purity of F-labeled FAPI compounds 18 The present application discloses a method for detecting the radiochemical purity of F-labeled FAPI compounds, which comprises using thin layer chromatography to detect the radiochemical purity of F-labeled FAPI compounds 18 The present application discloses a method for detecting the radiochemical purity of F-labeled FAPI compounds, which comprises using thin layer chromatography to detect the radiochemical purity of F-labeled FAPI compounds 18 The present application discloses a method for detecting the radiochemical purity of F-labeled FAPI compounds, which comprises using thin layer chromatography to detect the radiochemical purity of F-labeled FAPI compounds

The invention discloses a Sorosil decoction specific chromatogram and a construction method and application thereof, and belongs to the technical field of traditional Chinese medicine analysis and detection. The method comprises the following steps: respectively dissolving reference substances of bergenin, arbutin, glycyrrhizic acid, liquiritin, liquiritigenin and gallic acid in a solvent to obtain reference substance solutions; adding soroxil decoction into a solvent, carrying out ultrasonic extraction, and filtering to obtain a test solution; and injecting the reference solution and the test solution into a high performance liquid chromatograph for detection, taking the specific chromatogram of the reference solution as a reference chromatogram, selecting common peaks from the specific chromatogram of the test solution, and constructing the specific chromatogram of the soroxil decoction. The invention also discloses a Sorosil decoction characteristic spectrum and application thereof. According to the specific chromatogram of the soroxi decoction, rapid identification of the formula of the soroxi decoction can be realized, the method is simple, the research blank of the specific chromatogram in quality control of the classic famous-prescription soroxi decoction and the preparation thereof is filled up, and a powerful guarantee is provided for quality improvement of the classic famous-prescription soroxi decoction.

The invention relates to a detection method for residual DCC and DCU in a docetaxel raw material. The method uses a high performance liquid chromatography method to detect DCC and DCU in the docetaxel raw material. The method comprises the following steps: 1, preparing a test solution of the docetaxel raw material; 2, preparing a DCC reference solution and a DCU reference solution; 3, injecting samples, and detecting the test solution in step 1 and the reference solution in step 2 respectively according to the following conditions: chromatographic conditions: mobile phase: acetonitrile-water; gradient elution: the acetonitrile ratio in 0-5min is 15% isocratic washing; the acetonitrile ratio in 0-30min is gradually increased from 15% to 55%, and the acetonitrile ratio in 30min-60min is increased from 55% to 85%; the detection wavelength is 205nm; 4, the residual DCC and DCU in the test sample are calculated by using an external standard method based on the peak area of ​​the reference solution. The detection method for the content of DCC and DCU in the docetaxel raw material provided by the invention has low toxicity of the reagents used, less interference from impurities, and reliable and stable detection method.

The invention discloses a separation method of nystatin and components thereof. The method comprises the step of determining nystatin and components thereof in a test sample by using a high performance liquid chromatograph. The separation and detection method disclosed by the invention is good in separation degree, excellent in peak pattern and simple to operate, and also has good method specificity and sensitivity.

The invention belongs to the technical field of pesticide residue detection, and relates to a method for detecting residues of four herbicides in farmland soil through QuEChERS pretreatment-high performance liquid chromatography-tandem mass spectrometry. The method comprises the following specific steps: (1) drawing a standard curve: preparing a mixed standard solution of four pesticides including topramezone, bicyclopyrone, isoxaflutole and dicarfentrazone, carrying out high performance liquid chromatography-tandem mass spectrometry analysis, and drawing the standard curve according to the concentrations of the four pesticide standard solutions and quantitative ion pair peak areas; (2) QuEChERS pretreatment: performing solvent extraction, salt extraction and purifying agent treatment on to-be-detected soil to obtain a to-be-detected sample solution; and (3) analyzing the concentration of the pesticide residues: performing high performance liquid chromatography-tandem mass spectrometry analysis on the sample solution to be detected, and calculating to obtain the concentration of the four pesticide residues in the soil to be detected. The method is simple in pretreatment, high in detection recovery rate, good in detection result repeatability and high in detection sensitivity.

The invention belongs to the technical field of medical analysis, and particularly relates to a method for detecting the content of polyethylene glycol substances, the LC-MS method adopted by the detection method has the advantages that the use amount of a sample is small, the response of each selected ion is high, the peak pattern is good, proper ions can be screened out, and the detection accuracy is high. The problem of content detection of polyethylene glycol substances with similar molecular weights and similar structures is effectively solved.

The invention provides a method and a kit for detecting blood concentration of compound sulfamethoxazole and metabolite thereof. According to the method, a methanol precipitator is combined with Oasis PRiME HLB reversed-phase solid-phase extraction, the centrifugal process of plasma treatment is reduced, traditional activation and balance steps are omitted, time is saved, meanwhile, the method is more environmentally friendly, and the extraction recovery rate of a to-be-detected substance can be improved; gradient elution is replaced by isocratic elution, the balance returning time is avoided, the overall operation time is shorter, the separation application is not influenced, the operation time is only 6 minutes, all the to-be-measured substances can be accurately and simultaneously measured only by 1 microliter, and the peak appearance time is 2 minutes. According to the invention, trimethoprim, sulfamethoxazole and metabolites thereof sulfamethoxazole-N-acetyl and 4-nitro sulfamethoxazole in human plasma can be monitored at the same time by using a treatment method and a computer method.

The invention discloses a platycodon electron beam irradiation sterilization and quality detection method, which belongs to the field of Chinese medicine sterilization and quality detection of Chinese medicine, and comprises the following steps: (1) establishing a fingerprint of platycodon; (2) sealing and packaging the platycodon in a packaging bag, and irradiating the packaged platycodon using a high-energy electron accelerator; (3) storing the irradiated platycodon at room temperature; (4) performing quality detection on the irradiated platycodon. The application can significantly reduce the number of microorganisms in platycodon; the test results show that after 360 days of storage, the number of microorganisms in the irradiated platycodon meets the requirements of the pharmacopoeia, and the sensory properties and physical and chemical indicators thereof are not significantly changed. At the same time, under the same chromatographic conditions, the application not only establishes the fingerprint of platycodon, but also simultaneously determines the content of platycodon saponin D in platycodon. The whole method is simple to operate and has both qualitative and quantitative functions. The application overcomes the singleness of the original quality detection method and has a high application value.

The present invention relates to a method for detecting azacitidine impurities, and belongs to the technical field of quality control of azacitidine. The present invention provides a method for detecting azacitidine impurities, using liquid chromatography, wherein the stationary phase is uncapped octadecylsilane bonded silica gel, the mobile phase is water-acetonitrile=(85-92):(8-15), v / v, and the ion pair reagent sodium octane sulfonate is added to the water phase. Compared with the prior art, the present detection method has the advantages of being able to simultaneously detect three azacitidine impurities, thereby saving analysis costs, high sensitivity, simple operation, separation and accuracy meeting requirements, good impurity peak symmetry, and greatly reducing the concentration of the test sample, thereby extending the tolerance and service life of the chromatographic column.

The invention belongs to the technical field of quality detection of traditional Chinese medicine preparations, and particularly provides a fingerprint spectrum construction method and a quality detection method of a liver regulating decoction compound preparation. According to the fingerprint spectrum construction method of the liver regulating decoction compound preparation, octadecylsilane chemically bonded silica is used as a filling agent; an acetonitrile-phosphoric acid-containing aqueous solution is used as a mobile phase for gradient elution, an elution procedure is obtained through repeated tests, under the elution condition of the method, the number of common peaks (up to 22) is remarkably increased, the common peaks are good in peak shape and separation effect, a base line is stable, and the method is suitable for large-scale industrial production. Therefore, the quality of the liver regulating decoction compound preparation can be detected more comprehensively, clearly and effectively.

The present invention provides a cilnidipine optical isomer impurity content detection method, which comprises: detecting cilnidipine optical isomer impurities by using a high performance liquid chromatography to obtain a liquid chromatogram, and calculating according to the liquid chromatogram to obtain the cilnidipine optical isomer impurity content, wherein a mobile phase used in the high performance liquid chromatography comprises methanol and water; a chromatographic column used in the high performance liquid chromatography is selected from Formes Speedcore PFP (Polyfluoropropylene). The method is high in detection efficiency and accuracy, good in impurity and principal component peak shape and capable of achieving baseline separation.

The application provides a kind of Fuling drink piece, fried Fuling drink piece and the construction method and application of its preparation characteristic map, the construction method of fried Fuling and its preparation characteristic map, comprising: preparing test sample solution;Using high performance liquid chromatography to detect test sample solution, chromatographic conditions include with octadecylsilane bonded silica as filler, with methanol as mobile phase A, with 0.08%-0.4% formic acid as mobile phase B, and using specific elution procedure, while shortening the detection time also significantly improves the separation effect of multiple effective components, so that the characteristic map contains more characteristic peaks, greatly enriches the atlas information, realizes the effective separation of characteristic peaks such as uridine, thymine, gallic acid and guanosine, the characteristic of the obtained characteristic map is obvious, fully reflects the integrity and characteristic of the drug preparation of fried Fuling, and the baseline is stable, the peak shape is good, which provides basis for comprehensively detecting the quality of Fuling drink piece, fried Fuling and its pharmaceutical preparations.

The present invention belongs to the technical field of veterinary drug detection, and specifically relates to a method for simultaneously detecting multiple medicinal components in Yuhuang oral liquid by UPLC-PDA. The present invention can simultaneously determine the contents of 10 components in Yuhuang oral liquid, including berberine hydrochloride, bamipine hydrochloride, baicalin, gardenia glycoside, gallic acid, paeoniflorin, rhein, rhein, physophanol, and chrysophanol, thereby simultaneously detecting 7 medicinal components, including coptis root, phellodendron, scutellaria baicalensis, gardenia jasminoides, terminalia chebula, white peony root, and rhubarb. The present invention uses spectrum and chromatography for qualitative and quantitative determination, which is rapid, sensitive, accurate, and highly specific. Through one sample processing, the contents of 10 components can be simultaneously determined, which not only saves reagents and time, but also improves work efficiency.

The invention discloses a detection method for ellagic acid and its metabolites, and relates to the field of detection and analysis technology. The method of the present invention optimizes the pre-treatment method and detection technology, and adopts a composite extractant N-methylpyrrolidone-methanol solution to ultrasonically extract substances rich in ellagic acid and urolithin, and the extracted samples are qualitatively and quantitatively determined by high performance liquid chromatography tandem ultra-high resolution mass spectrometer, and at the same time, according to the experimental results, a simple high performance liquid chromatography determination method is developed. This method breaks the shortcomings of the traditional detection method that ellagic acid and urolithin substances have poor solubility and are difficult to extract such substances in the sample, and the quantitative results are inaccurate. The method has simple processing steps, good separation effect, short detection time, high sensitivity, qualitative accuracy, simple operation, good specificity, and can analyze the changing trend of the ellagic acid metabolic pathway, providing new ideas and methods for studying its biological metabolic process.

The invention discloses a method for detecting aceclodine in a biological sample and application of the aceclodine in pharmacokinetics. The detection method comprises the following steps: taking a biological sample, adding 0.1-10 times of an aqueous solution containing a stabilizer into the biological sample, and uniformly mixing to obtain a biological sample solution; quantitatively transferring the biological sample solution, sequentially adding the internal standard substance working solution and the organic solvent, uniformly mixing in a vortex manner, and centrifuging; quantitatively transferring the obtained supernatant, adding pure water, uniformly mixing by vortex, centrifuging, and taking the supernatant to obtain a biological sample test solution; preparing a series of vinegar clinidine standard test solutions for the standard curve; and performing test analysis on the biological sample test solution and a series of vinegar clinidine standard test solutions for the standard curve by liquid chromatography-tandem mass spectrometry (LC-MS / MS). According to the invention, the method for detecting the aceclodine in the biological sample is applied to the pharmacokinetic research of the aceclodine in the living body, so that the technical blank in the aspect of aceclodine detection at present is filled up.

The invention provides a construction method and a quality detection method of a characteristic spectrum of a traditional Chinese medicine composition, and the construction method of the characteristic spectrum comprises the following steps: taking a solution of a traditional Chinese medicine composition test sample and a solution of a reference substance, and carrying out high performance liquid chromatography detection to obtain the characteristic spectrum of the traditional Chinese medicine composition; wherein the traditional Chinese medicine composition comprises radix scutellariae, cortex mori radicis, fructus trichosanthis, semen armeniacae amarae, radix adenophorae, radix ophiopogonis, semen coicis, semen benincasae, fructus aurantii, radix curcumae, rhizoma imperatae, radix salviae miltiorrhizae, indigo naturalis and clam shells; the liquid chromatographic analysis conditions of the detection are as follows: octadecylsilane chemically bonded silica is used as a filler, acetonitrile is used as a mobile phase A, a phosphoric acid solution with the volume percentage of 0.09-0.11% is used as a mobile phase B, gradient elution is performed, the detection wavelength is 207-286nm, the column temperature is 25-35 DEG C, and the flow velocity is 0.9-1.1 mL / min. The specific chromatogram constructed by the invention can objectively evaluate the quality of a traditional Chinese medicine composition sample and improve the quality of the traditional Chinese medicine composition, so that the curative effect is ensured.

The invention relates to the technical field of pharmaceutical analysis, in particular to a method for determining the content of ferulic acid in a caulis sargentodoxae standard decoction and a method for constructing a specific chromatogram of the ferulic acid in the caulis sargentodoxae standard decoction. High performance liquid chromatography is adopted for determination, and the conditions of the high performance liquid chromatography are as follows: octadecylsilane chemically bonded silica is taken as a filling agent of a chromatographic column; acetonitrile is used as a mobile phase A, a phosphoric acid aqueous solution with the volume percentage of 0.1-0.3% is used as a mobile phase B, and gradient elution is carried out. The method is high in specificity, good in repeatability and durability and high in stability and accuracy; the constructed specific chromatogram and the detection method can rapidly and comprehensively realize the purpose of combined control of multiple components in the standard decoction of the stephania lancea, realize quality control of the stephania lancea from medicinal materials to preparations, and are beneficial to guarantee of effectiveness of clinical medication.

The application discloses a kind of shentong zhuyu decoction fingerprint construction method and application, belong to the technical field of drug detection.The application is detected by high performance liquid chromatography to several batches of shentong zhuyu decoction by the selection of mobile phase and the cooperation of chromatographic column, the high performance liquid chromatography of different batches of shentong zhuyu decoction is compared, obtains the common peak containing loganic acid, amygdalin, hydroxyl safflower yellow pigment A, aniseed acid, qianghuo alcohol, isoeucommarin, ammonium glycyrrhizinate, kaempferol characteristic peak, and the fingerprint of shentong zhuyu decoction is constructed according to the common peak.The fingerprint constructed by the application can better detect 12 medicinal materials, shorten the detection time of single sample to 125 min or less, improve efficiency, and improve the quality control level of product.

The invention belongs to the technical field of traditional Chinese medicine detection, and specifically provides a method for determining the content of chemical components in a Banxia Baizhu Tianma decoction. The raw materials of the Banxia Baizhu Tianma decoction include Pinellia, Gastrodia, Atractylodes, Tangerine Peel, Poria, Licorice, Ginger and Jujube, and the method comprises the following steps: S1, preparing a test solution; S2, preparing a reference solution with gastrodin and p-hydroxybenzyl alcohol as reference substances; S3, respectively determining the chromatograms of the test solution and the reference solution by high performance liquid chromatography; refer to the specification for details, the method for determining the content of chemical components in the Banxia Baizhu Tianma decoction provided by the invention adopts acetonitrile and an aqueous solution containing phosphoric acid as mobile phases, and elution is performed under a specific gradient, so as to achieve complete separation of two effective components, namely gastrodin and p-hydroxybenzyl alcohol, and the content testing method has the advantages of short detection time, high separation degree, strong specificity, good peak shape, linear relationship meeting requirements, and high precision, accuracy and stability.

The present invention discloses a method for constructing a fingerprint of a gui nourishing blood preparation, and the fingerprint is a method for constructing a HPLC characteristic fingerprint of a gui nourishing blood preparation. The present invention establishes a method for constructing a HPLC characteristic fingerprint of a gui nourishing blood preparation, which overcomes the singleness and one-sidedness of existing quality standards; it can fully reflect the quality information of the gui nourishing blood preparation, thereby achieving the purpose of more comprehensively and effectively controlling the product quality of the gui nourishing blood preparation. For some active ingredients that cannot be identified and the effective ingredients are not clear, the fingerprint method can be used to prove the consistency of product quality. The fingerprint of the gui nourishing blood preparation obtained by the method of the present invention has many peaks, good peak shape, easy identification, high similarity, and is accurate and reliable. The method of the present invention is simple to operate, stable, and repeatable, and the conclusion is objective and accurate.

The invention belongs to the technical field of traditional Chinese medicine detection, and particularly relates to a specific chromatogram construction method and application of a Xiangbai powder compound preparation, and the specific chromatogram construction method comprises the following steps: (1) preparing a test solution; (2) the test solution is taken and detected by adopting a high performance liquid chromatography, octadecylsilane chemically bonded silica is taken as a filler, a mobile phase comprises acetonitrile and an aqueous solution containing phosphoric acid, a gradient elution procedure comprises 0-10 min, 12 min, 32 min, 60 min, 70 min, 90 min, 93 min, 98 min, 99 min and 105 min, and the volume percentage of acetonitrile in the mobile phase is 5%-5%-7%-25%-50%-60%-80%-5%-5%. According to the method, effective separation of 12 common characteristic peaks including the boxthorn A peak can be realized, and the obtained characteristic spectrum is stable in baseline, good in peak shape of the characteristic peaks and high in separation degree.

The present invention provides a method for determining the content of components of a skin disease blood poison pill, a method for constructing a fingerprint spectrum and its application, and relates to the technical field of quality detection methods for traditional Chinese medicine preparations. The determination method comprises the following steps: weighing a reference substance, mixing it with a solvent, and preparing a mixed reference substance solution; preparing a test solution; drawing the reference substance solution and the test solution, respectively injecting them into a liquid chromatograph, determining, and obtaining a chromatogram; the two methods use the same high-performance liquid chromatography conditions and are applied to the quality control of skin disease blood poison pill preparations, which is faster and more efficient than using two methods to detect fingerprints and contents respectively. Not only the contents of 7 components of gallic acid, chlorogenic acid, paeoniflorin, berberine hydrochloride, arctiin, paeonol and rubiacein in the skin disease blood poison pill were determined, but also the constructed fingerprint spectrum calibrated 54 peaks. This method can more comprehensively and systematically reflect the quality of the skin disease blood poison pill than the determination of the content of a single component.

This application discloses a method for detecting astaxanthin content. The provided method includes the following steps: mixing the sample to be tested with acetone to prepare a test solution; performing high-performance liquid chromatography (HPLC) on the test solution. The HPLC conditions include: using tert-butyl methyl ether as mobile phase A and a mixed solution of methanol and acetonitrile as mobile phase B, with isocratic elution. The provided detection method uses HPLC, employing a mixed solvent of tert-butyl methyl ether and methanol-acetonitrile as the mobile phase, and successfully achieves efficient and rapid separation of astaxanthin through isocratic elution. The chromatographic peak shape is excellent, effectively overcoming interference from proteins, lipids, nutrients, and ionic components in a complex matrix, providing a reliable analytical tool for assessing the intestinal absorption characteristics of astaxanthin.

The present invention relates to the technical field of traditional Chinese medicine analysis, and specifically to a method for constructing a fingerprint spectrum of Yangwei Kangliu Granules and its fingerprint spectrum. The method adopts the HPLC-DAD-ELSD method, wherein the preparation method of the test solution comprises first adding water to part of the Yangwei Kangliu Granules, ultrasonically treating, extracting with water-saturated n-butanol, and combining the n-butanol solution; then taking the remaining Yangwei Kangliu Granules, ultrasonically treating with solvent 1, filtering, and obtaining a filtrate; finally, mixing part of the filtrate with the n-butanol solution, evaporating to dryness, and dissolving the residue with solvent 2. The method of the present invention is simple to operate, accurate and reliable, and has the characteristics of good precision, stability and repeatability. It can overcome the shortcomings of the prior art that the detection index is single and cannot reflect the intrinsic quality of the process control of the Yangwei Kangliu Granules, and realize a comprehensive and accurate evaluation of the preparation quality, thereby more effectively ensuring the preparation quality.