638 results about "Chromatographic column" patented technology

The invention provides embedded metal fullerene and a preparation method thereof. The preparation method comprises the following steps: mixing a metal source and graphite powder, and filling a hollow graphite rod with the mixture to prepare an anode; the electrode is installed at the anode end of an electric arc furnace, and on-line activation is conducted through anode and cathode short-circuit current under the inert atmosphere; then carrying out arc discharge under specific air pressure and current, and collecting generated carbon ash; performing ultrasonic extraction by using an organic solvent to obtain a fullerene crude extracting solution; and finally, performing multi-stage separation and purification by high performance liquid chromatography, and performing primary and fine separation by sequentially adopting preparative and semi-preparative chromatographic columns to obtain a high-purity product. According to the method, a complete technical path capable of efficiently and controllably preparing the ultra-high-purity embedded metal fullerene is formed through a synthesis process integrating online activation and accurate atmosphere regulation and control and a multi-stage gradient chromatography purification strategy, and a solid foundation is laid for application of atomic-scale accurate materials in the frontier fields of quantum devices and the like.

The invention belongs to the technical field of traditional Chinese medicine detection, and discloses a detection method for a characteristic spectrum of Uighur medicine Kspecific heat extracting tablets and application of the detection method. The detection method provided by the invention comprises the following steps: preparing a test sample solution by taking a 75% methanol solution as a solvent, and determining the test sample solutions prepared from different batches of test samples by adopting ultra-high performance liquid chromatography to obtain a characteristic spectrum; the chromatographic conditions of the ultra-high performance liquid chromatography are as follows: a chromatographic column is a C18 chromatographic column, a mobile phase A is acetonitrile, a mobile phase B is a 0.1 vol% phosphoric acid solution, and the flow velocity is 0.25-0.35 mL / min; the detection wavelength is 0 to 18 minutes, and the wavelength is 327 nm; the time is 18 to 28.5 min, and the wavelength is 254 nm; when the time is 28.5 to 37.6 minutes, the wavelength is 242 nm; after 37.6 to 70 minutes, the wavelength is 210 nm; the sample size is 1 microliter, the column temperature is 20-40 DEG C, and gradient elution is carried out. The invention provides a more scientific identification and quality control method for the Kespecific heat tablets.

The invention relates to the field of pharmaceutical analytical chemistry, in particular to a method for determining tandospirone and salt intermediates thereof, high performance liquid chromatography is adopted, and chromatographic conditions include that a chromatographic column with octadecylsilane chemically bonded silica as a filler is adopted, and gradient elution is performed by a mobile phase A and a mobile phase B; wherein the mobile phase A consists of a first water phase and acetonitrile; the mobile phase B is composed of a second water phase and acetonitrile; the first water phase is a water solution containing monopotassium phosphate and sodium hexanesulfonate, and the pH value of the first water phase is 3.0-3.8; the second water phase is an aqueous solution containing monopotassium phosphate and sodium hexanesulfonate, and the pH value of the second water phase is 1.8-2.6. The testing method disclosed by the invention has good specificity, accuracy and repeatability, and can be used for monitoring the quality of the tandospirone raw material medicine.

The invention provides a method for detecting free amino acids in camellia oleosa seed oil, and establishes a method for detecting the free amino acids in the camellia oleosa seed oil based on ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS / MS), which comprises the following steps: pre-treating various amino acids in the camellia oleosa seed oil by using a non-derivation method; the problems of complicated operation, impurity interference and the like caused by derivatization are avoided, the extraction solvent is environment-friendly and economical, the extraction efficiency is high, and the pretreatment time is greatly shortened; then selecting a proper chromatographic column and a mobile phase, separating a plurality of free amino acids under the condition of not introducing excessive derivatization agents and ion pair reagents, and controlling the collection time to be 12 minutes. The method for detecting the free amino acids in the camellia oleosa seed oil has the characteristics of no need of derivatization treatment, simplicity and convenience in operation, high detection efficiency and good sensitivity and accuracy, and can effectively separate and detect 14 free amino acids such as alanine, phenylalanine and aspartic acid in the camellia oleosa seed oil.

The invention relates to a precise separation and online detection integrated system for a multi-component complex system, and belongs to the technical field of analysis and detection. The system comprises an intelligent preprocessing and component sensing unit, a self-adaptive precise separation unit, a multi-modal cooperative detection unit, a zero dead volume-intelligent adaptive interface unit and a multi-algorithm fusion intelligent control and data analysis unit, and the five units form a closed-loop cooperative architecture through real-time data interaction. The system adopts a preprocessing-sensing integrated design, a multi-mode switching type separation architecture, a dynamic intelligent chromatographic column, a four-channel collaborative detection system and multi-algorithm fusion regulation. According to the invention, the problems of separation and detection disjunction, narrow adaptability, weak matrix interference suppression capability, low intelligent degree, poor portability and the like in the prior art are effectively solved, and one-time sample injection accurate separation and high-accuracy detection of trace target components with wide polarity range and similar structures can be realized.

The invention belongs to the technical field of detection methods, and particularly relates to a liquid chromatography detection method for an idedecalciferol intermediate and impurities thereof. According to the liquid chromatography detection method for the eldecalciferol intermediate and the impurities thereof, a liquid chromatography method is combined with an electrospray detector for detection; chromatographic conditions are as follows: an amylose-tri (3-chlorphenyl carbamate) covalently bonded silica gel chromatographic column is adopted; and carrying out gradient elution by taking water as a mobile phase A and acetonitrile as a mobile phase B. The method for detecting the eldecalciferol intermediate and the impurities of the eldecalciferol intermediate is good in specificity, high in sensitivity and accuracy, good in repeatability and stability and high in analysis speed, the content of the eldecalciferol intermediate and the impurities of the eldecalciferol intermediate can be rapidly and accurately detected, and the blank of detection of the eldecalciferol intermediate and the impurities of the eldecalciferol intermediate in the prior art is filled.

The invention discloses a CAD detection method for phosphoric acid and phosphorous acid in an alendronate sodium oral solution, and belongs to the technical field of pharmaceutical analysis. The method comprises the following steps: preparing a reference stock solution and a test solution; then setting high performance liquid chromatography detection conditions; a Shodex VN-504D chromatographic column is adopted, a CAD detector is adopted as a detector, a mixed solution of a mobile phase A and a mobile phase B is adopted as a mobile phase, the mobile phase A is ammonium formate, the mobile phase B is an acetonitrile solution, and gradient elution is performed by adopting the mobile phase; and respectively measuring the reference stock solution and the test solution, injecting into a liquid chromatograph, and recording a chromatogram. A CAD detector is adopted, the detection sensitivity is obviously improved, the quantitative concentration can reach 0.24 mu g / mL, and the detection concentration can reach 0.10 mu g / mL. The method is high in specificity, high in sensitivity, good in durability, rapid, simple, effective and reliable.

The invention relates to the field of biological product preparation, in particular to a preparation method of high-activity oyster-derived small molecule peptide. The method comprises the following steps: selecting fresh meat of pacific oysters or ostrea rivularis as a raw material, degreasing, adding water, crushing, filtering, sequentially carrying out compound protease and neutral protease hydrolysis, implanting a sensor and a probe in hydrolysis, monitoring in real time, and dynamically adjusting reaction conditions; adding a targeting microcapsule wrapping an ascorbic acid glutathione composite protective agent, and releasing the protective agent when a target peptide signal is detected; separating and concentrating through a gradient membrane, and purifying the target peptide by using a molecular imprinting chromatographic column; carrying out alkaline hydrolysis on enzymatic hydrolysis residues to prepare chitosan for cyclic utilization, carrying out enzymatic hydrolysis on residual residues to generate collagen peptide, treating and recycling wastewater, and recovering protease. According to the method, the technical effects that the high-activity and high-purity target peptide is prepared, the resource utilization rate is increased, the production cost is reduced, the target peptide has good oxidation resistance and biological activity, the intestinal enzyme degradation rate is reduced after glycosylation modification, and the blood half-life period is prolonged are achieved.

The invention discloses a method for detecting the content of nicotinamide mononucleotide, ergothioneine and nicotinamide. According to the method, the content of nicotinamide mononucleotide, ergothioneine and nicotinamide in a sample is analyzed by adopting a high performance liquid chromatography; according to the method, a CAPCELL PAK ADME chromatographic column is used, and the molecular weight of the CAPCELL PAK ADME chromatographic column is 100 And an acetonitrile-buffer solution is used as a mobile phase. Chromatographic peaks obtained by the method are sharp and symmetrical in shape, good in area reproducibility, good in separation degree, simple, rapid, high in specificity, short in detection period and high in sensitivity; the contents of nicotinamide mononucleotide, ergothioneine and nicotinamide in a sample can be rapidly, efficiently, accurately and simultaneously detected.

The application belongs to the technical field of natural medicinal chemistry, and discloses a benzofuran compound in Jinyunming and a preparation method and application thereof. The benzofuran compound is separated from Jinyunming of the plant Dalbergia hupeana Tutch in the family of Leguminosae. The heartwood of Jinyunming is extracted and concentrated to obtain extract, the extract is sequentially separated by multiple silica gel column chromatography, medium-pressure ODS column chromatography and gel column chromatography, and then purified by semi-preparative high-performance liquid chromatography, so that the benzofuran compound can be obtained. The application establishes an inflammation model by inducing RAW 264.7 cells with lipopolysaccharide (LPS), and shows that the compound has good anti-inflammatory activity, the IC50 is 14.33 μM, and the expression of inflammatory factors interleukin-6 (IL-6) and interleukin-1β (IL-1β) can be inhibited.

The invention relates to the technical field of analysis and detection, and discloses a construction method of a fingerprint spectrum of relevant characteristic peaks of rhizoma coptidis paste and radix rehmanniae recen, and the construction method comprises the following steps: S1, preparing a reference solution and a test solution; and S2, injecting the reference substance solution and the test solution into a high performance liquid chromatograph, adopting a chromatographic column with octadecylsilane chemically bonded silica as a filler, taking acetonitrile as a mobile phase A, taking a 0.0125 mol / L monopotassium phosphate solution as a mobile phase B, eluting according to a specified gradient program, and detecting by adopting an ultraviolet detector to obtain the fingerprint spectrum. By means of the provided construction method of the fingerprint spectrum of the relevant characteristic peaks of the coptis chinensis paste and the radix rehmanniae recen, the sequence and the mutual relation of the characteristic peaks can be indicated, and the effect of the fingerprint spectrum of the relevant characteristic peaks of the coptis chinensis paste and the radix rehmanniae recen is effectively represented.

The application provides a method for establishing a fingerprint spectrum of Wenshoujieyu granules and the fingerprint spectrum, and relates to the technical field of traditional Chinese medicine preparation detection. The method for establishing the fingerprint spectrum adopts high performance liquid chromatography to detect the Wenshoujieyu granules, and in the method, the chromatographic conditions are as follows: Agilent 5TC-C 18 (2) (4.6mm*250nm, 5um) is used as a chromatographic column; acetonitrile and 0.1% formic acid aqueous solution are used as mobile phases; the flow rate is 1mL*min ‑1 ; the column temperature is 45 DEG C; the injection amount is 15ul; and the detection wavelength is 300nm. The method for detecting the fingerprint spectrum of the Wenshoujieyu granules established by the application is simple in operation, reliable in result, high in stability and precision, and good in repeatability; the fingerprint spectrum provided by the application can be used to comprehensively and effectively control the quality of the preparation, and ensure the stability, reliability and safety of the preparation.

This invention discloses an analytical method for benzothiazole contaminants, comprising the following steps: detection by high performance liquid chromatography (HPLC), with the following chromatographic conditions: a phenylhexyl column as the stationary phase, mobile phase A being water, mobile phase B being acetonitrile, and gradient elution; wherein the benzothiazole contaminants are at least one of 2-aminobenzothiazole, 2-hydroxybenzothiazole, benzothiazole, 2-methylbenzothiazole, 2-mercaptobenzothiazole, 2,5-dimethylbenzothiazole, 2-cyanobenzothiazole, ethyl 2-carboxylate benzothiazole, 2-chlorobenzothiazole, 2-bromobenzothiazole, 2-methylthiobenzothiazole, phenylthiocyanate, N-tert-butyl-2-benzothiazole sulfenamide, 2-(2-hydroxyphenyl)-benzothiazole, N-cyclohexyl-2-benzothiazole sulfinamide, dibenzothiazole disulfide, and N,N-dicyclohexyl-2-benzothiazole sulfonamide.

The invention relates to a method for detecting genotoxic impurities of rupatadine fumarate, which is characterized in that a phosphate buffer solution is matched with acetonitrile, a high performance liquid chromatography method is adopted, a chromatographic column is a silica gel bonded octadecylsilane column, a gradient program is adopted for elution, and the content of the rupatadine fumarate genotoxic impurities in the rupatadine fumarate genotoxic impurities in the rupatadine fumarate genotoxic impurities is detected. Effective separation between rupatadine fumarate and genotoxic impurities and between genotoxic impurities can be achieved, accurate quantitative analysis can be achieved, and the quality of drugs can be effectively controlled.

The invention belongs to the technical field of pharmaceutical analysis, and discloses a method for determining two potential genotoxic impurities in carbon [13C]-urea by gas chromatography. Comprising the following steps: (1) preparing a sample solution; (2) preparing a reference solution; (3) taking nitrogen as carrier gas, and detecting by adopting a medium-polarity or weak-polarity chromatographic column separation system; and (4) calculating by a peak area through an external standard method to obtain the accurate contents of methyl carbamate and ethyl carbamate in the carbon [13C]-urea. The method disclosed by the invention is simple to operate, good in specificity, linearity, precision and accuracy and high in sensitivity, and can realize accurate detection of residual potential genotoxic impurities, namely methyl carbamate and ethyl carbamate, in the carbon [13C]-urea.

The application discloses a method for analyzing the purity of heptafluoropropane extinguishing agent, which comprises the following steps: (1) configuring heptafluoropropane and impurity standard gas; (2) selecting a chromatographic column type; (3) setting gas chromatograph analysis conditions; and (4) detecting a sample to be measured. Compared with the prior art, the method has the advantages of simple operation, better separation effect and higher accuracy.

The invention belongs to the technical field of pharmaceutical analysis, and particularly relates to a method for separating and determining nafamostat mesylate SM2 and related impurities thereof. The method is a high performance liquid chromatography, and comprises the following steps: by taking octadecylsilane chemically bonded silica as a chromatographic column filler, a potassium hexafluorophosphate solution as a mobile phase A and acetonitrile as a mobile phase B, separating nafamostat mesylate SM2 and related impurities thereof through linear gradient elution; and detecting by using detection wavelengths of 200 nm to 240 nm and 250 nm to 280 nm to obtain a chromatogram, so that effective determination of the nafamostat mesylate SM2 and the related impurities thereof is realized. The method provided by the invention adopts common buffer salt as a mobile phase, and has the characteristics of short analysis time, strong specificity, high sensitivity and good reproducibility.

The invention relates to the technical field of pharmaceutical analysis, and particularly discloses a high performance liquid chromatography-mass spectrometry tandem method (HPLC-MS / MS) for detecting a genotoxic impurity L-alanine isopropyl ester in an emtricitabine-propofol tenofovir tablet. By optimizing chromatographic column selection, a mobile phase gradient elution procedure and mass spectrometric detection parameters, the method realizes exclusive, sensitive and accurate detection of the L-isopropyl alanine. The methodological verification result shows that the detection limit is 1.00 ng / mL, the quantification limit is 2.00 ng / mL, the linear range is 2.00-100.00 ng / mL (the correlation coefficient r is equal to 0.9994), the recovery rate is stabilized between 92% and 98%, and the precision and repeatability are good. The detection method has the advantages of high sensitivity and strong selectivity, is suitable for quality control of emtricitabine propofol tenofovir tablets, meets the control requirements of ICH M7 guide on genotoxic impurities, and has good practicability and popularization value.

The present application relates to camellia oil high-value processing technology and application field, specifically relates to a kind of process for preparing colorless camellia oil by silica gel chromatography.It is characterized in that: different silica gel adsorbent and activated carbon are pretreated according to specific combination to build chromatographic column, to achieve the purpose of preparing cosmetic colorless camellia oil from cold-pressed camellia oil, degumming and deacidification camellia oil, refined camellia oil or edible camellia oil at room temperature.The present application effectively avoids the technical defects of high temperature and repeated physical adsorption decolorization in traditional process, has the advantages of raw material safety and environmental protection, simple process, significant energy saving, high preparation efficiency;Using adsorption chromatography to remove pigment components, the quality of cosmetic grade colorless camellia oil is achieved, a new preparation path of colorless camellia oil is opened up, which can provide technical support for the high-quality development of camellia oil industry.

The invention discloses a quantitative detection method for a mixture of bis (2-hydroxyethyl) disulfide and mercaptoethanol, and relates to the field of analysis and detection. The testing method comprises the following steps: respectively preparing a bis (2-ethoxyl) disulfide standard solution and a mercaptoethanol standard solution with different concentrations; and quantitatively measuring the standard solution and the detection sample through gas chromatography equipment, optimizing chromatographic conditions, drawing a standard curve according to the detection result of the standard solution, and quantitatively analyzing the detection sample by combining the standard curve and the spectrum of the standard solution. According to the present invention, the gas chromatography conditions are optimized, the phenyl functional group-containing polysiloxane is selected as the filler of the chromatographic column, and the detection temperature and the temperature rising program are controlled so as to ensure the separation and the detector sensitivity, and the split ratio is controlled to avoid the non-linear response and the peak overlapping; the detection accuracy of the bi-component bis (2-ethoxyl) disulfide and mercaptoethanol is high, the chromatographic peak separation degree is good, and the product quality detection requirement can be met.

The invention relates to the technical field of rare earth hydrometallurgy, and discloses a process for preparing ultra-high-purity rare earth oxide by an ion exchange method, which comprises the following steps: loading monodisperse polystyrene sulfonic acid resin into a chromatographic column, and carrying out constant-temperature circulating pretreatment by using a pre-swelling solution containing urea and ammonium nitrate; preparing a rare earth feed liquid containing urea and with anions being nitrate radicals, introducing the rare earth feed liquid into the chromatographic column for adsorption saturation, and then connecting the rare earth feed liquid in series with the retardation column; carrying out displacement leaching by using a composite leaching solution in which ethylenediamine tetraacetic acid, 5-sulfosalicylic acid and urea are dissolved, and collecting target rare earth effluent in sections; and finally, adding a precipitant into the effluent for precipitation, and filtering, washing and firing to obtain the ultra-pure rare earth oxide. According to the invention, a urea swelling driven dynamic compensation system is constructed, and 10%-14% of monodisperse resin with high crosslinking degree is selected to cooperate with urea-ammonium nitrate pretreatment, so that low-pressure-drop operation at a high flow rate of 1.0 BV / h or above is realized.

The present application relates to the field of pharmaceutical analysis, and particularly relates to a method for determining the content of cetirizine hydrochloride and preservatives in cetirizine hydrochloride oral solution. The method provided by the present application uses octadecyl bonded silica as the filler of the chromatographic column, uses a mixed solution with specific composition and proportion as the mobile phase, adopts isocratic elution, uses a content volumetric pipette to prepare the test sample solution, injects the test sample solution and the mixed control solution into the liquid chromatograph respectively to perform liquid chromatography, thereby obtaining the chromatogram of the test sample solution and the chromatogram of the mixed control solution respectively; and then according to the external standard method, the content of cetirizine hydrochloride, the content of the hydroxy methylbenzoate preservative and the content of the hydroxy propylbenzoate preservative in the cetirizine hydrochloride oral solution are calculated by the peak area. The method has strong specificity, high accuracy, good precision, linear relationship and stability, and is conducive to the evaluation of sample quality.

The application relates to the technical field of analytical chemistry, in particular to a high-performance liquid chromatography analysis method for maleic acid olmutant, wherein the mobile phase in the high-performance liquid chromatography condition comprises mobile phase A and mobile phase B, the mobile phase A is a mixed solution, the mixed solution comprises trifluoroacetic acid, triethylamine and water, the volume fraction of trifluoroacetic acid in the mixed solution is 0.08-0.12%, the volume fraction of triethylamine is 0-0.3%, the mobile phase B is acetonitrile, and the volume ratio of the mobile phase A and the mobile phase B is 75:25-85:15. The trifluoroacetic acid and the triethylamine in the mobile phase are matched, the interaction between maleic acid olmutant and a chromatographic column is hindered, the peak shape is symmetrical, and peak tailing is significantly improved; meanwhile, the two can also form a buffer system; in addition, the trifluoroacetic acid can enhance the retention capacity of maleic acid olmutant on the chromatographic column and improve separation selectivity.

The utility model discloses a special oil-resistant anti-pollution chromatographic column for transformer oil, which relates to the technical field of staining chromatographic columns and comprises a first fixing ring fixedly mounted at the upper end of the outer part of a staining chromatographic column shell. The internal mounting ring can rotate along with the appearances of the three protective guide pieces, so that the internal mounting ring can transversely rotate while longitudinally sliding, the decontamination cloth can uniformly wipe the exterior of the dyeing column shell, stains are prevented from being attached to the dyeing column shell, and the device is prevented from being damaged when the device is taken down and stored. Dust and dirt outside the dyeing column shell enter the device from the connecting port, and meanwhile, the device can be supported by the three protection guide pieces, so that the dyeing column shell is not in contact with the outside, the supporting and protection effects are achieved, it is guaranteed that the outer portion of the dyeing column shell is not stained with some dust, and it is guaranteed that the outer portion of the dyeing column shell is clean.

The invention relates to a method for analyzing and detecting a non-azonetin enantiomer, which adopts normal-phase high performance liquid chromatography and takes normal hexane-ethanol-trifluoroacetic acid as a mobile phase to detect the non-azonetin enantiomer, and the specific chromatographic conditions are as follows: a chromatographic column is prepared by covalently bonding amylose-tri (3, 4, 6-trimethyl-1, 3-pentanediol) on the surface of silica gel; 2, 5-dichlorophenyl carbamate is used as a filling agent (IE, 4.6 * 250 mm, 5.0 [mu] m); a mobile phase: n-hexane: ethanol: trifluoroacetic acid = 500: 500: 1 (V / V / V); the flow rate is 1.0 ml / min; the column temperature is 30 DEG C; the sample size is 20 [mu] l; the detection wavelength is 280 nm; the elution gradient is isocratic elution. The invention provides a high performance liquid chromatography method for analyzing the enantiomer of the fiazonetin, the complete separation of the fiazonetin and the enantiomer of the fiazonetin can be realized by adopting the method, and the separation degree of a main component and the enantiomer is greater than 1.5.

The invention discloses a fractionation method of an endocrine disrupter in an environmental water body, and belongs to the technical field of water body pollutant analysis. The fractionation method of the endocrine disrupter in the environmental water body comprises the following steps: S1, enriching and concentrating a water sample to obtain a to-be-fractionated liquid; s2, fractionating the to-be-fractionated liquid by adopting an analytical liquid chromatography system and a C18 semi-preparative chromatographic column to obtain fractions. According to the method, high-resolution synchronous fractionation of the endocrine disrupter in a wide polarity range (Log Kw:-0.19-6.62) is realized, the defects of low fractionation efficiency and high solvent consumption of the traditional semi-preparative liquid chromatography are effectively overcome by optimizing a gradient program of an analytical liquid chromatography system, the time and economic cost are saved, and the method is suitable for industrial production. And the fraction can be directly compatible with biological activity test after salting-out-extraction treatment.

The invention relates to the technical field of vegetable and fruit pesticide residue detection, in particular to a method and a system for separating and determining ethiprole enantiomer residue in grapes by ultra performance convergence chromatography. The method comprises the following steps: extracting a sample twice by 1% acetic acid acetonitrile, performing rotary evaporation concentration, redissolving by dichloromethane / methanol, purifying by adopting an amino solid-phase extraction column, concentrating, fixing the volume by isopropanol / n-heptane (1: 9), and filtering. The method comprises the following steps: separating by using a Trefoil CEL2 chiral chromatographic column, carrying out gradient elution at 35 DEG C and 10.3 MPa by using supercritical CO2 as a main mobile phase and 0.5% ammonia water methanol as a modifier, detecting PDA at 280 nm, and carrying out external standard quantification. The method is rapid in separation and good in peak shape, the linear range is 0.5-20.0 mg / L, the LOQ is 0.1 mg / kg, the adding standard recovery rate is 80.0%-108%, the RSD is 3.1%-8.4%, and the method is suitable for monitoring ethiprole enantiomer residues in grapes.

The invention relates to the technical field of medicine detection, in particular to a separation method of N-fluorenylmethoxycarbonyl-N '-trityl-L-histidine and related substances. The invention provides a method for analyzing the purity of N-fluorenylmethoxycarbonyl-N '-trityl-L-histidine and related substances, which comprises the following steps: high performance liquid chromatography is adopted, and the chromatographic conditions are as follows: a filler of a chromatographic column is pentafluorophenyl silica gel; the mobile phase A is an aqueous solution containing 0.1 wt% of phosphoric acid (the pH is adjusted to 3.7 by using ammonia water); the mobile phase B is acetonitrile: methanol (1: 1, V / V); a gradient elution procedure; according to the method, the Fmoc-His (Trt)-OH and the related substances as well as the related substances can be effectively separated, the separation degree is greater than or equal to 1.5, the peak shape symmetry is better, the detection sensitivity of each related substance is high, the content (purity) detection result of each component is accurate and effective, and the quality of the Fmoc-His (Trt)-OH can be correctly reflected.