1503 results about "Chromatographic column" patented technology

A construction method for and a use of rhizoma pinellinae praeparata decoction piece, standard decoction and formula granule characteristic spectra. The construction method comprises the following steps: dissolving a test sample by using a solvent, and extracting to obtain a solution to tested; and measuring said solution by using high-performance liquid chromatography (HPLC) to obtain rhizoma pinellinae praeparata decoction piece, standard decoction and formula granule characteristic spectra, wherein chromatographic conditions of the HPLC are as follows: the chromatographic column is a C18 column; and gradient elution is performed by using acetonitrile as mobile phase A and a 0.1% phosphoric acid aqueous solution as mobile phase B. HPLC is used, gradient elution is performed by using acetonitrile and a 0.1% phosphoric acid aqueous solution as mobile phases, and 4-hydroxybenzoic acid is used as a reference object, so that rhizoma pinellinae praeparata decoction piece, standard decoction and formula granule HPLC characteristic spectra are established; the method has good repeatability and precision, is stable and reliable, and can control the quality of rhizoma pinellinae praeparata decoction pieces, a standard decoction and formula granules.

The invention provides a preparation method and application of boron isotope separation resin microspheres. The preparation method comprises the following steps: (1) adding dimethyl sulfoxide into polystyrene-divinylbenzene resin microspheres to swell for 3 hours, adding concentrated sulfuric acid to treat until the sulfonation rate is 0.3-0.4 meq / g, washing with water, treating with an ammonium chloride aqueous solution, washing with water, drying in vacuum, atomizing and spraying a ferric chloride-ferrous chloride aqueous solution, evaporating in vacuum to remove part of water, and introducing ammonia-containing nitrogen flow to treat to obtain polystyrene-divinylbenzene resin microspheres; and washing with water, grinding the surface, treating with a sodium hydroxide aqueous solution and washing with water. The boron isotope separation resin microspheres are suitable for chromatographic separation of 10B and 11B in a boric acid-sodium hydroxide aqueous solution with the pH of 6-7.5, a desorption solution is pure water, the feeding rate is 0.3-0.6 m / h, and a boric acid-containing aqueous solution enriched with 10B can be obtained; the service life of the chromatographic column is more than 6 months, the adsorption separation performance is reduced by not more than 20%, and the chromatographic column has a certain application prospect.

The invention relates to the technical field of drug analysis and detection, and provides a detection method of moxifloxacin hydrochloride related substances. The related substances of moxifloxacin hydrochloride are detected by adopting a high performance liquid chromatography method, and the detection conditions are as follows: octadecylsilane chemically bonded silica is taken as a filling agent of a chromatographic column; the wavelength of a UV detector is 296 nm; the column temperature is 35-45 DEG C; the flow velocity of the mobile phase is 1.6-1.8 ml / min; the sample injection volume is 10-50 [mu] l; the mobile phase A is phosphate buffer solution-acetonitrile-methanol; the mobile phase B is phosphate buffer solution-acetonitrile-methanol; the elution mode is gradient elution; the diluent is a mobile phase A; according to the method provided by the invention, effective separation between moxifloxacin hydrochloride and various impurities can be realized, the sensitivity is high, the accuracy is good, the reproducibility is relatively good, a good linear relation is shown in a certain range, and qualitative and quantitative determination can be carried out on various impurities in moxifloxacin hydrochloride bulk drugs.

The invention relates to the technical field of deep-sea microorganism nucleic acid detection, in particular to a long-term experimental platform for deep-sea microorganism in-situ nucleic acid detection and a detection method. According to the invention, a water sample is subjected to tangential flow filtration and concentration through the filtration and sampling module, so that a concentrated microorganism sample is obtained. The method comprises the following steps of: transferring a sample into an amplification detection module under reduced pressure, transferring into a lysis purification module for cell lysis, injecting the cracked sample into a chromatographic column, filtering impurities and intercepting DNA by the chromatographic column, then washing by using an eluent to obtain a high-purity DNA sample, loading a suction head through a transfer washing liquid gun, mixing the high-purity DNA sample with an enzyme solution, adding into the amplification detection module for reaction, and detecting the DNA in the amplification detection module. And carrying out fluorescence labeling on the amplified gene, obtaining an amplification curve by using a fluorescence detector, and detecting the in-situ nucleic acid of the deep-sea microorganisms according to the change of the amplification curve. An automatic cleaning assembly is designed in the filtering and sampling module, and the cracking and purifying module is used for sampling and cleaning a cracking chamber and switching pipelines, so that cross contamination caused by deep sea in-situ multiple detection is prevented.

The invention discloses purified gastrodia elata polysaccharide as well as a preparation method and application thereof, and belongs to the technical field of extraction of active ingredients of gastrodia elata. The method comprises the following steps: (1) adding an alcoholic solution into gastrodia elata powder for reflux extraction, and collecting filter residues; (2) dissolving the filter residues in pure water, and performing ultrasonic extraction to obtain filtrate; (3) adding absolute ethyl alcohol into the filtrate for low-temperature alcohol precipitation to obtain crude gastrodia elata polysaccharide; (4) performing deproteinization treatment on the gastrodia elata crude polysaccharide; (5) purifying by using macroporous resin; (6) dialyzing the crude polysaccharide purified liquid in pure water; (7) purifying by using a first chromatographic column, wherein a filler is cellulose-based anion exchange resin; (8) purifying by using a second chromatographic column to obtain purified gastrodia elata polysaccharide; and the filler is sephadex. According to the present invention, the gastrodia elata crude polysaccharide is purified by using the five purification means, such that the high-purity gastrodia elata purified polysaccharide can be rapidly, stably and efficiently obtained.

The invention belongs to the technical field of medicine detection, and particularly relates to a high-sensitivity liquid chromatography method for detecting mutagenic impurities in atomoxetine hydrochloride key starting material 3-chlorobenzene propanol, the method adopts an HPLC (High Performance Liquid Chromatography) analysis method, a chromatographic column adopts octadecylsilane chemically bonded silica as a filler, and the composition of a mobile phase A is a buffer solution; the mobile phase B is methanol or acetonitrile; carrying out gradient elution on the mobile phase; the flow velocity is 0.4 to 0.8 ml / min; the detection wavelength is 230 to 250 nm; by adopting an HPLC analysis method, the content of mutagenic impurities in the key starting material 3-chlorobenzene propanol of atomoxetine hydrochloride can be effectively separated and measured, and the method is high in sensitivity, strong in specificity and good in accuracy and repeatability, and has extremely important significance on quality control of the key starting material 3-chlorobenzene propanol of atomoxetine hydrochloride.

The invention relates to the technical field of sugar detection in dairy products, and discloses a method for determining sugar in dairy products by using ultra-high performance liquid chromatography-mass spectrometry, and the method comprises the following steps: establishing a regression equation of various sugar standard substances by using ultra-high performance liquid chromatography-mass spectrometry; removing protein in the dairy product sample by using acetic acid to obtain a dairy product sample without the protein; removing lipids in the dairy product sample without the proteins by using a solid-phase extraction small column to obtain a dairy product sample without the proteins and the lipids; detecting the dairy product sample without proteins and lipids by using ultra-high performance liquid chromatography-mass spectrometry, and carrying out qualitative and quantitative analysis on sugar in the dairy product sample according to the regression equation; wherein in the ultra-high performance liquid chromatography-mass spectrometry, two amino chromatographic columns are connected in series. According to the method provided by the invention, as many as 20 saccharides and sugar alcohols in the dairy product can be accurately determined, and the 20 saccharides and sugar alcohols in the dairy product can be qualitatively and quantitatively determined.

The invention provides a content detection method for a salvia miltiorrhiza medicinal material, a salvia miltiorrhiza clear paste and a musk heart-dredging dropping pill. According to the method, a high performance liquid chromatography method is adopted, octadecylsilane chemically bonded silica is used as a chromatographic column filler, acetonitrile is used as a mobile phase A, 0.02%-0.1% trifluoroacetic acid is used as a mobile phase B, gradient elution is carried out, and rosmarinic acid and salvianolic acid B in the salvia miltiorrhiza medicinal material are synchronously and quantitatively detected. And synchronously and quantitatively detecting tanshinol, protocatechuic aldehyde, caffeic acid, salvianolic acid E, rosmarinic acid, alkannic acid and salvianolic acid B in the salvia miltiorrhiza clear paste, and synchronously and quantitatively detecting tanshinol, protocatechuic aldehyde, caffeic acid, salvianolic acid E, rosmarinic acid, alkannic acid, salvianolic acid B, arenobufagin and bufalin in the musk Tongxin dropping pill. The method is used for monitoring the content of specific components, can also judge the transfer rate in the medicine production process, and facilitates traceability in the production link. In addition, the method is relatively low in equipment requirement and suitable for industrial popularization.

The invention provides embedded metal fullerene and a preparation method thereof. The preparation method comprises the following steps: mixing a metal source and graphite powder, and filling a hollow graphite rod with the mixture to prepare an anode; the electrode is installed at the anode end of an electric arc furnace, and on-line activation is conducted through anode and cathode short-circuit current under the inert atmosphere; then carrying out arc discharge under specific air pressure and current, and collecting generated carbon ash; performing ultrasonic extraction by using an organic solvent to obtain a fullerene crude extracting solution; and finally, performing multi-stage separation and purification by high performance liquid chromatography, and performing primary and fine separation by sequentially adopting preparative and semi-preparative chromatographic columns to obtain a high-purity product. According to the method, a complete technical path capable of efficiently and controllably preparing the ultra-high-purity embedded metal fullerene is formed through a synthesis process integrating online activation and accurate atmosphere regulation and control and a multi-stage gradient chromatography purification strategy, and a solid foundation is laid for application of atomic-scale accurate materials in the frontier fields of quantum devices and the like.

The invention provides a therapeutic drug monitoring kit and method and system, a magnetic bead extraction method is combined with LC-MS / MS detection to be applied to human blood drug concentration monitoring, a mixed magnetic bead solution containing phospholipid removal magnetic beads and to-be-detected drug adsorption magnetic beads is utilized to perform adsorption and extraction pretreatment on a to-be-detected drug in a sample, automatic treatment equipment is matched, and the concentration of the to-be-detected drug in the to-be-detected drug in the to-be-detected drug in the to-be-detected drug in the to-be-detected drug in the to-be-detected drug in the to-be-detected drug is detected. The pretreatment efficiency is high, the phospholipid content in the supernate is low, and the matrix effect and the chromatographic column washing pressure are greatly reduced. According to the therapeutic drug monitoring method provided by the invention, a unified pretreatment process is used, simultaneous extraction of multiple drugs is realized, and through a magnetic bead automatic extraction method, the pretreatment step of a sample can be rapidly completed, a therapeutic drug monitoring result can be rapidly obtained, the overall detection time is shortened, and the detection efficiency is improved. Therefore, a reliable laboratory examination basis is provided for individualized treatment.

The invention provides a method for detecting nitrosamine impurities in a starting material N-Boc-piperazine of tandospirone citrate, which comprises the following steps: carrying out linear gradient elution on a chromatographic column by taking octadecylsilane chemically bonded silica as a filler, a potassium hexafluorophosphate solution as a mobile phase A and acetonitrile as a mobile phase B; the pH value of the potassium hexafluorophosphate solution ranges from 4.3 to 5.5. The method is high in specificity, high in detection sensitivity and accuracy and good in linearity, and the nitrosamine impurity 1-nitrosopiperazine contained in N-Boc-piperazine can be accurately measured. According to the method, the technical barrier of detecting the nitrosamine impurities by using a gas chromatography-mass spectrometry or liquid chromatography-mass spectrometry method at present is overcome, the detection cost is reduced, and meanwhile, other impurities contained in the method do not influence the accurate detection of the nitrosamine impurities.

The invention belongs to the technical field of traditional Chinese medicine detection, and discloses a detection method for a characteristic spectrum of Uighur medicine Kspecific heat extracting tablets and application of the detection method. The detection method provided by the invention comprises the following steps: preparing a test sample solution by taking a 75% methanol solution as a solvent, and determining the test sample solutions prepared from different batches of test samples by adopting ultra-high performance liquid chromatography to obtain a characteristic spectrum; the chromatographic conditions of the ultra-high performance liquid chromatography are as follows: a chromatographic column is a C18 chromatographic column, a mobile phase A is acetonitrile, a mobile phase B is a 0.1 vol% phosphoric acid solution, and the flow velocity is 0.25-0.35 mL / min; the detection wavelength is 0 to 18 minutes, and the wavelength is 327 nm; the time is 18 to 28.5 min, and the wavelength is 254 nm; when the time is 28.5 to 37.6 minutes, the wavelength is 242 nm; after 37.6 to 70 minutes, the wavelength is 210 nm; the sample size is 1 microliter, the column temperature is 20-40 DEG C, and gradient elution is carried out. The invention provides a more scientific identification and quality control method for the Kespecific heat tablets.

The invention belongs to the technical field of pharmaceutical analysis, and relates to a method for detecting the content of povidone K30. According to the method, the content of povidone K30 in the medicinal preparation is detected by adopting a high performance liquid chromatography; detection conditions of the high performance liquid chromatography are as follows: a chromatographic column is a C18-AQ column; a mobile phase is a phosphate solution. The method has the characteristics of high accuracy, strong specificity and the like for detecting the content of povidone K30 in the pharmaceutical preparation, can overcome the interference of other auxiliary materials in the pharmaceutical preparation, and has important application value in development and consistency evaluation of the pharmaceutical preparation.

The invention relates to the field of pharmaceutical analytical chemistry, in particular to a method for determining tandospirone and salt intermediates thereof, high performance liquid chromatography is adopted, and chromatographic conditions include that a chromatographic column with octadecylsilane chemically bonded silica as a filler is adopted, and gradient elution is performed by a mobile phase A and a mobile phase B; wherein the mobile phase A consists of a first water phase and acetonitrile; the mobile phase B is composed of a second water phase and acetonitrile; the first water phase is a water solution containing monopotassium phosphate and sodium hexanesulfonate, and the pH value of the first water phase is 3.0-3.8; the second water phase is an aqueous solution containing monopotassium phosphate and sodium hexanesulfonate, and the pH value of the second water phase is 1.8-2.6. The testing method disclosed by the invention has good specificity, accuracy and repeatability, and can be used for monitoring the quality of the tandospirone raw material medicine.

An exemplary machine learning-based system and method are disclosed for a clinical tool that can provide a comprehensive prediction of ovarian cancer propensity using metabolite and metabolomic profiles acquired from ultra-performance liquid chromatography, high-resolution mass spectrometry (UPLC-MS) analysis. Each serum sample may be independently processed through two different columns (HILIC and C18 reversed phase). Different ionization modes (negative and positive) may be used. The clinical tool can validated to provide successful prediction of ovarian cancer by over 90%. Ultra-performance liquid chromatography coupled with tandem mass spectrometry (UPLC-MS / MS) and the like can be used.

The invention relates to the technical field of medicine production and detection, and particularly discloses a method for determining the content of paeoniflorin in radix astragali and cassia twig Wuwei decoction granules, which comprises the following steps: analyzing paeoniflorin by high performance liquid chromatography, and sequentially performing sample pretreatment, chromatographic analysis and content determination. An HP-20 resin column is adopted for pretreatment, ethanol with different concentrations is used for elution in sequence, and 50% and 70% ethanol eluents are collected and concentrated; a C18 chromatographic column is selected, an acetonitrile-0. 1% phosphoric acid aqueous solution is used as a mobile phase, a diode array detector is used for detection at the wavelength of 230 nm, and a multi-wavelength deconvolution algorithm is used for quantitative analysis. The determination method disclosed by the invention is high in specificity, stability, repeatability, intermediate precision, accuracy and the like all accord with regulations, and the durability of chromatographic conditions is relatively good.

The invention discloses a construction method of a gout compound decoction fingerprint as well as the fingerprint and application thereof, and belongs to the technical field of analysis and detection. The construction method of the fingerprint spectrum comprises the following steps: taking a test solution prepared from the gout compound decoction; the test solution is subjected to HPLC-SPD detection, so that the fingerprint spectrum of the gout compound decoction is obtained, and the HPLC detection conditions are that a C18 chromatographic column is used, a C18 chromatographic column is used, a C18 chromatographic column is used, a C18 chromatographic column is used, and a C18 chromatographic column is used for detecting the fingerprint spectrum of the gout compound decoction. An aqueous solution containing 0.1%-0.2% of phosphoric acid is used as a mobile phase A, and acetonitrile is used as a mobile phase B for gradient elution; the flow velocity is 0.8-1 mL / min, the column temperature is 25 DEG C, the sample injection volume is 5-10 [mu] L, and a diode array detector is 190-400 nm full-wave band scanning. The chemical components in the gout compound decoction are comprehensively and systematically analyzed, and a basis is provided for quality control and in-depth study of a pharmacodynamic material basis.

The invention relates to the technical field of agricultural product detection, in particular to an agricultural product pesticide residue detection method and system. According to the method, the spatial orientation characteristics of molecular three-dimensional conformation parameters are introduced, a symmetry index set is established, matching calculation is carried out in combination with temperature distribution in chromatographic column operation parameters and the sample injection direction, accurate linkage of structural information and a chromatographic path is achieved, and through coupling analysis of response starting time and chromatographic regulation and control parameters, the accuracy of the chromatographic regulation and control parameters is improved. A detection time window taking a target protein signal as a reference is set, a response enhancement fragment with a significant rising rate is screened out in combination with a signal change trend in the window, and a target signal interval is obtained through weighted fitting, so that the specificity and pertinence of detection data are effectively improved, and the detection accuracy is improved through comparison of peak position time and response data. According to the scheme, accurate labeling of pesticide types in a complex sample is achieved, and the method has high stability and judgment accuracy under the scene that the sample heterogeneity is high and signal interference is complex.

The invention discloses a detection method for determining N-nitroso-Vonoprasan in Vonoprasan fumarate, and belongs to the technical field of medicine detection.The detection method comprises the steps that a solution is prepared, and detection is conducted; the detection comprises the following steps: determining a test solution and a reference solution by adopting a liquid chromatography-mass spectrometry method, recording a chromatogram, and calculating the content of N-nitroso-Vonorazan according to the chromatogram and an external standard method; according to the method, a triple quadrupole rod liquid phase mass spectrometer is adopted; a chromatographic column adopts octadecyl silane bonded silica gel as a filling agent, the specification of the chromatographic column is Poroshell 120EC-C18, 1.9 mu m, 50 mm * 3.0 mm; a mobile phase is composed of a mobile phase A and a mobile phase B, and gradient elution is adopted; the ultra-high pressure chromatographic column is adopted, the operation time is shorter, the signal-to-noise ratio is higher, the detection sensitivity is higher, the detection cost is lower, and the detection requirement can be met on a low-end triple quadrupole mass spectrum.

The invention discloses a method for detecting melogabalin besylate and isomer impurities of melogabalin besylate. According to the method, liquid chromatography is adopted, an FXAA reagent and a sample to be tested are subjected to a reaction under the alkaline condition to obtain a solution to be detected, then the solution to be detected is subjected to liquid chromatography separation, the structural formula of the FXAA reagent is # imgabs0 #, R is selected from-CH (CH3) 2,-CH2CH2SCH3 or-C6H5, and the structural formula of the FXAA reagent is as shown in the specification, the structural formula of the FXAA reagent is as shown in the specification, and the structural formula of the FXAA reagent is as shown in the specification, the structural formula of the FXAA reagent is as shown in the specification, the structural formula of the FXAA reagent is as shown in the specification, and the structural formula of the FXAA reagent is as shown in the specification. The test sample comprises melogabalin besylate and isomer impurities thereof, and the liquid chromatography adopts a reversed-phase chromatographic column. According to the method, melogabalin besylate and three isomer impurities thereof can be safely and effectively separated and respectively detected, the detection sensitivity, repeatability and durability are good, and a powerful guarantee is provided for quality control of the medicine.

The invention discloses a synchronous monitoring system and method for non-methane total hydrocarbon and volatile organic compound components, and belongs to the technical field of environmental monitoring. Comprising an adsorption tube and a monitoring assembly, the adsorption tube is detachably connected with the monitoring assembly, the monitoring assembly comprises a focusing tube, a six-way valve, a four-way valve and a gas chromatograph, and the four-way valve and the six-way valve are each provided with an emptying flow path; the six-way valve is respectively communicated with the focusing tube and the capillary chromatography sampling assembly, and the capillary chromatography sampling assembly, the capillary chromatographic column and the mass spectrometry detector are sequentially communicated; a heating assembly is arranged outside the adsorption tube, a temperature control device is arranged outside the focusing tube, a thermal desorption temperature control area is arranged outside the focusing tube, the six-way valve and the four-way valve, and a column oven is arranged outside the capillary chromatographic column. Synchronous monitoring of non-methane hydrocarbon and VOCs components is achieved through the same monitoring assembly, the occupied space is reduced, the monitoring range is expanded, meanwhile, the sampling volume of the adsorption tube is flexible and controllable, and the sample preservation time is long.

The invention relates to a method for detecting the content of amino acid in a pesticide through pre-column derivatization liquid chromatography, and relates to the technical field of chemical analys.The method comprises the steps that a pesticide sample to be detected is extracted, and a solution containing amino acid is obtained; then, under the optimized alkaline pH and heating conditions, 4-fluoro-7-nitro-2, 1, 3-benzoxadiazole (NBD-F) is adopted as a derivatization reagent to be subjected to a pre-column derivatization reaction with amino acid, and a stable high-fluorescence NBD-F derivative is formed; injecting the derivative liquid into a high performance liquid chromatography system provided with a phenyl bonded phase chromatographic column, and efficiently separating the NBD-F derivative by utilizing pi-pi interaction and hydrophobic interaction of the phenyl column; and finally, detecting and quantifying through a fluorescence detector. Through the synergistic effect of NBD-F derivation and the phenyl column, the separation selectivity and the detection sensitivity are remarkably improved, and the reaction efficiency and the derivative stability are guaranteed through optimized derivation conditions.

The present invention provides a kind of detection method of thirteen kinds of related substance contents in vonoprazan fumarate, and high performance liquid chromatography is used for detection, and the chromatographic conditions are: the test solution and the reference solution are injected into the high performance liquid chromatograph, and the chromatographic conditions are: the chromatographic column adopts octadecyl bonded silica gel column; the ultraviolet detector is used, and the detection wavelength is 260nm; the injection volume: 10μl; the column temperature: 30℃‑40℃; the flow rate: 0.8ml / min‑1.0ml / min; the gradient elution method is used, and the mobile phase A: the solution of 0.02mol / L potassium dihydrogen phosphate solution adjusted to pH 8.0 with potassium hydroxide solution and isopropanol mixed with a volume ratio of 95:5; the mobile phase B: the mixed solution of acetonitrile and isopropanol with a volume ratio of 95:5. The method can be used to detect the contents of 13 kinds of related substances in vonoprazan fumarate, and high performance liquid chromatography is used for detection, and the method has high detection sensitivity, and the verification results show that the system applicability and specificity of this method meet the requirements, and the needs of drug production quality monitoring can be met.

The invention provides a method for detecting free amino acids in camellia oleosa seed oil, and establishes a method for detecting the free amino acids in the camellia oleosa seed oil based on ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS / MS), which comprises the following steps: pre-treating various amino acids in the camellia oleosa seed oil by using a non-derivation method; the problems of complicated operation, impurity interference and the like caused by derivatization are avoided, the extraction solvent is environment-friendly and economical, the extraction efficiency is high, and the pretreatment time is greatly shortened; then selecting a proper chromatographic column and a mobile phase, separating a plurality of free amino acids under the condition of not introducing excessive derivatization agents and ion pair reagents, and controlling the collection time to be 12 minutes. The method for detecting the free amino acids in the camellia oleosa seed oil has the characteristics of no need of derivatization treatment, simplicity and convenience in operation, high detection efficiency and good sensitivity and accuracy, and can effectively separate and detect 14 free amino acids such as alanine, phenylalanine and aspartic acid in the camellia oleosa seed oil.

The invention relates to the field of polymer grading, and discloses a polyethylene grading method, polyethylene obtained through the polyethylene grading method, a system for polyethylene grading and application of the system. According to the polyethylene grading method, on the basis of preparing a high-temperature liquid chromatographic column, two-time flow division is adopted, and the flow ratio of second flow division is controlled, so that accurate and directional grading of a polyethylene raw material is realized, and the purposes of accurate and directional regulation and control of the average molecular weight, the molecular weight distribution curve and the branched structure of polyethylene, especially low-density polyethylene, are realized.

The invention discloses a total component and a blood-entering component of a herba artemisiae scopariae dampness-clearing granule and a detection method of the total component and the blood-entering component. The preparation method comprises the following steps: A, decocting the herba artemisiae scopariae dampness-clearing granules, filtering decoction to obtain filtrate, and concentrating the filtrate to obtain concentrated liquid; b, detecting the concentrated solution by adopting liquid chromatography-mass spectrometry; the liquid chromatography conditions are as follows: a reversed-phase C18 chromatographic column is adopted, the column temperature is 40 DEG C, a mobile phase A is formic acid water with the volume fraction of 0.1%, a mobile phase B is acetonitrile, and the flow velocity is 0.3 ml / min. The research investigates all components of the dampness-clearing prescription, i.e., the rhizoma smilacis glabrae dampness-clearing granules, and investigates blood entering components of the dampness-clearing prescription, i.e., the rhizoma smilacis glabrae dampness-clearing granules after long-term intervention in patients with the dampness-syndrome metabolic syndrome in clinical practice. A new modern theoretical system is injected for the research of traditional Chinese medicine dampness syndrome and dampness clearing prescription clinical intervention, and a reference is provided for the research of other syndromes of traditional Chinese medicine.

The invention discloses a method for extracting erythrinine from beautiful millettia roots by using a deep-eutectic solvent and a purification process. Wherein the optimal process for extracting the erythrinine is as follows: LA-Fruu (1: 1) with the water content of 40% is used under the conditions that the ultrasonic temperature is 80 DEG C, the ultrasonic time is 40 minutes and the liquid-material ratio is 20: 1, and the extraction amount of the erythrinine can reach 3.1495 + / -0.0690 mg / g; the optimal purification process is as follows: DM132 type macroporous resin is used, the eluent is 50% ethanol, the elution flow rate is 2.5 BV / h, the height-diameter ratio of a chromatographic column is 21: 1, the elution volume is 4BV, and the recovery rate of erythrinine is 89.53 + / -3.23%. According to the invention, a deep eutectic solvent and macroporous resin combined technology is established, the erythrinine is extracted and purified from the millettia specisoa champ in a high-efficiency, rapid, green and environment-friendly manner, and a green and efficient way is provided for the extraction and purification of the erythrinine.

The invention relates to a precise separation and online detection integrated system for a multi-component complex system, and belongs to the technical field of analysis and detection. The system comprises an intelligent preprocessing and component sensing unit, a self-adaptive precise separation unit, a multi-modal cooperative detection unit, a zero dead volume-intelligent adaptive interface unit and a multi-algorithm fusion intelligent control and data analysis unit, and the five units form a closed-loop cooperative architecture through real-time data interaction. The system adopts a preprocessing-sensing integrated design, a multi-mode switching type separation architecture, a dynamic intelligent chromatographic column, a four-channel collaborative detection system and multi-algorithm fusion regulation. According to the invention, the problems of separation and detection disjunction, narrow adaptability, weak matrix interference suppression capability, low intelligent degree, poor portability and the like in the prior art are effectively solved, and one-time sample injection accurate separation and high-accuracy detection of trace target components with wide polarity range and similar structures can be realized.

The invention relates to the technical field of analytical chemistry, and particularly discloses an HPLC (High Performance Liquid Chromatography) detection method for related impurities in a vitamin B6 injection. The invention provides an HPLC detection method for related impurities in vitamin B6 injection. The HPLC detection method comprises the following steps: carrying out separation detection on a to-be-detected sample by adopting a chromatographic column taking octadecylsilane chemically bonded silica as a filler and a mobile phase gradient elution method; the mobile phase A is 0.04% sodium pentane sulfonate solution-methanol with the volume ratio of 95: 5, and the pH value of the 0.04% sodium pentane sulfonate solution is adjusted to 3.0 by adopting phosphoric acid; the mobile phase B is methanol. The HPLC detection method provided by the invention can detect 13 impurities in the vitamin B6 injection at the same time, the detection specificity is high, the sensitivity is high, the separation degree is good, and the quality of the vitamin B6 injection can be effectively controlled when the detection method is applied to production of the vitamin B6 injection.

The invention discloses a high performance liquid detection method for letemovir content, and belongs to the technical field of pharmaceutical chemicals. The method comprises the following steps: (1) preparing a sample; (2) detection: carrying out isocratic elution by adopting a chromatographic column taking octadecyl bonded silica gel as a filler, taking an ammonium dihydrogen phosphate solution with the concentration of 20mmol / L as a mobile phase A and taking acetonitrile-methanol (80: 20) as a mobile phase B; and (3) analysis: recording a chromatogram, and calculating the content of the letemovir component by a peak area according to an external standard method. According to the method, an isocratic elution detection method is selected, a base line is smooth, the requirement on an instrument is low, the detection time is short, the detection efficiency can be improved, and consumables and reagents are saved.