105 results about "Isocratic elution" patented technology

The invention relates to the technical field of food safety detection, in particular to a method for detecting urea in bean sprouts and application thereof.The method comprises the following steps that smashed bean sprouts are subjected to two times of ultrasonic extraction and centrifugation, supernate is combined, and an extracting solution is obtained; fixing the volume and filtering to obtain a to-be-detected solution; detecting the liquid to be detected by using liquid chromatography-tandem mass spectrometry; an extraction solvent for ultrasonic extraction is a 5% formic acid-acetonitrile composite solution; the conditions of the liquid chromatography-tandem mass spectrometry are as follows: the chromatographic conditions are as follows: a chromatographic column is an HILIC column; the sample size is 10 [mu] L; a mobile phase is a mixed solution of a 0.02 mmol / L ammonium acetate buffer salt solution and acetonitrile, and isocratic elution is carried out; the flow rate is 0.3 mL / min; the column temperature is 40 DEG C; mass spectrum conditions are as follows: an electrospray ionization (ESI) positive ion mode, electrospray voltage of 4000 V, sheath gas pressure of 30 psi, auxiliary gas pressure of 5 psi, capillary heating temperature of 300 DEG C and scanning mode of MRM. The method disclosed by the invention is free from substance interference, high in sensitivity, accurate in result, low in cost and high in efficiency, and can be used as a standard detection method.

The invention discloses a method for determining bedaquiline fumarate clean verification target residues, and relates to the technical field of instrument analysis of chemical pharmacy. The method comprises the following steps: preparing a sample wiping solution; determining the content of bedaquiline fumarate in the sample wiping solution by adopting a liquid chromatography; chromatographic conditions are as follows: a chromatographic column is a VP-ODS C18 column; mobile phases: a potassium hexafluorophosphate aqueous solution and acetonitrile, wherein the concentration of the potassium hexafluorophosphate aqueous solution is 0.01-0.03 mol / L; the elution mode is isocratic elution; the flow rate is 1 ml / min to 2 ml / min; the column temperature is 28-32 DEG C; the method has good specificity and sensitivity for determination of bedaquiline fumarate cleaning verification target residues, is simple and convenient to operate, rapid in determination, simple and easy to implement, and overcomes the defects that TOC, chemical titration methods, conductivity methods, pH methods and the like which are commonly used at present do not have specificity.

The invention relates to the technical field of plant extraction, and provides a plurality of phenolic compounds in senecio scandens, and a preparation method and application thereof. The method comprises the following steps: crushing senecio scandens, adding ethyl acetate, and performing ultrasonic heating, stirring and extracting to obtain an extracting solution; carrying out rotary evaporation and vacuum concentration on the extracting solution to obtain a crude extract; dissolving the crude extract by using absolute ethyl alcohol through ultrasonic heating, carrying out gradient elution through a macroporous adsorption resin chromatographic column, and collecting an ethanol water eluent; and concentrating the ethanol water eluent under reduced pressure, stirring with a reverse-phase filler, loading with a dry method, packing with a wet method, and sequentially performing isocratic elution with a reverse-phase chromatographic column and purification with a gel column. The dissolution efficiency of phenolic compounds in senecio scandens is improved through ultrasonic and mild heating, the problems that components are degraded due to high temperature in traditional reflux extraction and time and solvent are consumed in dipping extraction are solved, and target component residues are reduced; and then through gradient elution of a chromatographic separation column, isocratic elution of a reversed-phase chromatographic column and purification of a gel column, the problem that phenols are similar in structure and easy to co-elute is accurately solved.

The present application relates to the field of pharmaceutical analysis, and particularly relates to a method for determining the content of cetirizine hydrochloride and preservatives in cetirizine hydrochloride oral solution. The method provided by the present application uses octadecyl bonded silica as the filler of the chromatographic column, uses a mixed solution with specific composition and proportion as the mobile phase, adopts isocratic elution, uses a content volumetric pipette to prepare the test sample solution, injects the test sample solution and the mixed control solution into the liquid chromatograph respectively to perform liquid chromatography, thereby obtaining the chromatogram of the test sample solution and the chromatogram of the mixed control solution respectively; and then according to the external standard method, the content of cetirizine hydrochloride, the content of the hydroxy methylbenzoate preservative and the content of the hydroxy propylbenzoate preservative in the cetirizine hydrochloride oral solution are calculated by the peak area. The method has strong specificity, high accuracy, good precision, linear relationship and stability, and is conducive to the evaluation of sample quality.

The invention relates to a method for analyzing and detecting a non-azonetin enantiomer, which adopts normal-phase high performance liquid chromatography and takes normal hexane-ethanol-trifluoroacetic acid as a mobile phase to detect the non-azonetin enantiomer, and the specific chromatographic conditions are as follows: a chromatographic column is prepared by covalently bonding amylose-tri (3, 4, 6-trimethyl-1, 3-pentanediol) on the surface of silica gel; 2, 5-dichlorophenyl carbamate is used as a filling agent (IE, 4.6 * 250 mm, 5.0 [mu] m); a mobile phase: n-hexane: ethanol: trifluoroacetic acid = 500: 500: 1 (V / V / V); the flow rate is 1.0 ml / min; the column temperature is 30 DEG C; the sample size is 20 [mu] l; the detection wavelength is 280 nm; the elution gradient is isocratic elution. The invention provides a high performance liquid chromatography method for analyzing the enantiomer of the fiazonetin, the complete separation of the fiazonetin and the enantiomer of the fiazonetin can be realized by adopting the method, and the separation degree of a main component and the enantiomer is greater than 1.5.

The invention discloses a method for detecting phenyl sulfide in rectification residual liquid of 2, 4-dichlorophenol through high performance liquid chromatography, and belongs to the technical field of chemical detection and analysis. The method comprises the following steps: dissolving a to-be-detected 2, 4-dichlorophenol rectification residual liquid sample to prepare a to-be-detected sample, and preparing phenyl sulfide standard substance solutions with different concentrations; the method comprises the following steps: determining a to-be-detected sample and a phenyl sulfide standard substance solution by adopting a high performance liquid chromatograph, drawing a standard curve and a linear regression equation according to a detection result of the phenyl sulfide standard substance solution, and analyzing the phenyl sulfide in the sample by combining the linear regression equation and a chromatographic peak map of the to-be-detected sample, wherein the mobile phase comprises a phase A and a phase B, the phase A is an aqueous solution of phosphate buffer salt, the phase B is methanol, and an isocratic elution mode is adopted. According to the present invention, with the method, the accurate determination of the phenyl sulfide content in the 2, 4-dichlorophenol rectification residual liquid is achieved, the timely and accurate data guidance is provided for the 2, 4-D production process, and the target of production cost saving is ultimately achieved.

The invention relates to the technical field of pharmaceutical analysis, in particular to a chromatographic method for determining optical isomers in a carbidopa raw material medicine and a preparation thereof by using an HPLC (High Performance Liquid Chromatography) method. The detection method is simple, convenient, rapid and accurate to operate, and specifically comprises the following steps: dissolving a proper amount of a sample with a solvent, diluting to a certain concentration, taking a certain volume of the sample, carrying out isocratic elution at a certain column temperature by adopting a high performance liquid chromatography, using a hand-type chromatographic column, a mobile phase A and a mobile phase B, and carrying out quantitative analysis by adopting a self-control method with correction factors. And calculating the isomer in the test solution. The method is good in specificity, solution stability, linearity, repeatability, intermediate precision and durability, and the detection result is accurate and reliable through verification.

The application is suitable for the field of in-vitro diagnosis equipment, and discloses a liquid chromatograph. The liquid chromatograph comprises a liquid phase fluid conveying assembly, a chromatographic column, a detector and a controller; the liquid phase fluid conveying assembly comprises a storage component, a first driving component and a second driving component independent of the first driving component, the first driving component is used for driving a first liquid phase fluid from a first container to the storage component and driving a second liquid phase fluid from a second container to the storage component, so that the first liquid phase fluid and the second liquid phase fluid are mixed in the storage component at a preset ratio to form a third liquid phase fluid of a single concentration and are stored in the storage component; and the second driving component is used for driving the third liquid phase fluid to flow through the chromatographic column from the storage component, so that the third liquid phase fluid elutes a test liquid adsorbed on the chromatographic column. The application can realize the function of isocratic elution by using the liquid phase fluid prepared on line from two liquid phase fluids.

The invention belongs to the technical field of analytical chemistry, and discloses a method for detecting creatinine in a creatine product, which comprises the following steps: (1) precisely weighing a sample, adding a buffer solution, carrying out vortex oscillation dissolution, volume metering, shaking up, centrifuging, and passing through a water phase filter membrane to obtain a creatine test solution; (2) a creatine test solution is taken, a liquid chromatograph is adopted for liquid phase detection, and the liquid chromatography conditions are as follows: an SCX chromatographic column is adopted, an ammonium dihydrogen phosphate solution is taken as a mobile phase for isocratic elution, an ultraviolet detector is adopted, and the content of creatinine is obtained according to a liquid phase detection result. According to the method, the situation that the detection result of the creatinine in the creatine product is virtually high can be remarkably reduced, accurate quantification of the creatinine byproduct creatinine is achieved, and a more perfect data basis is provided for quality control of the creatine product.

The invention provides a method for simultaneously determining ritocaine and prilocaine in blood plasma based on a liquid chromatography-tandem mass spectrometry (LC-MSMS) technology. The specific operation of the method comprises the following steps: adding an internal standard solution and an organic solvent into a plasma sample, and ensuring full extraction of lidocaine and prilocaine through vortex mixing. And separating by using a centrifugal technology, and collecting supernate as a pretreated sample to be detected. An Eclipse Plus C18 chromatographic column is adopted for isocratic elution, effective separation of lidocaine and prilocaine is achieved, and then a sample is subjected to mass spectrometric detection. A standard curve is drawn based on the mass spectrum peak area ratio, a regression equation is obtained, and the concentration of lidocaine and prilocaine in the sample to be detected is obtained through calculation. The method has the characteristics of simplicity and convenience in pretreatment operation, low plasma consumption, short analysis time and high sensitivity, and is suitable for detection of lidocaine and prilocaine in plasma and clinical pharmacokinetic research of the lidocaine cream.

The invention discloses a method for detecting sialic acid in a dairy product. The method comprises the following steps: pretreating raw materials: weighing a uniformly mixed liquid dairy product, adding water for diluting, adding a precipitant consisting of potassium ferrocyanide and zinc acetate, uniformly shaking, adding water for fixing the volume, uniformly shaking, and filtering with a filter membrane of 0.5 mu m or less to obtain a filtrate; the concentration of the liquid dairy product is 1-10g / 100mL, the concentration of the potassium ferrocyanide is 0.1-0.26 g / 100mL, and the concentration of the zinc acetate is 0.2-0.55 g / 100mL after the water is added to a constant volume; a chromatographic column is a hydrogen type cation chromatographic column, a 0.005-0.01 mol / L sulfuric acid aqueous solution is used as an eluent for isocratic elution, and after elution, a PAD detector is adopted for detection under the detection wavelength of 210-220 nm. The method is suitable for detecting sialic acid in milk, and has the advantages of simple sample treatment, low treatment cost and accurate detection.

The invention provides a method for determining the content of two sesquiterpenoids in a turmeric extract, and belongs to the technical field of medicine detection.The quality detection method comprises the steps that the turmeric extract is taken to be prepared into a test solution, then acetonitrile (A) and 0.08-0.12 wt% phosphoric acid aqueous solution (B) are subjected to isocratic elution with the ratio of 65-75: 25-35, then an external standard one-point method or a standard curve method is used for determining the content of the two sesquiterpenoids in the turmeric extract, and the content of the two sesquiterpenoids in the turmeric extract is determined. And calculating the content of aryl turmerone and beta-turmerone in the turmeric extract. The method for determining the content of the two sesquiterpene compounds in the turmeric extract is innovatively established, accurate quantitative analysis of the target sesquiterpene component is realized for the first time by optimizing chromatographic separation conditions and detection parameters, the detection blank of an existing standard system is filled, and the method has a wide application prospect. And a key technical support is provided for constructing a turmeric extract multi-dimensional quality control system.

The application provides a qualitative and quantitative detection method of polynaphthalene formaldehyde sulfonate sodium salt in a pesticide preparation, comprising the following steps: S10, solution preparation: preparing a standard sample solution, a series of concentration standard solutions and a sample solution by using pure water as a solvent; S20, chromatographic detection: adopting high performance liquid chromatography, using a C18 chromatographic column as a stationary phase, using a mixed solution of a water solution containing sodium dodecyl sulfonate and acetonitrile as a mobile phase for isocratic elution, and detecting at a wavelength of 240 nm; S30, qualitative determination: comparing the chromatographic peak retention time of the standard sample solution and the sample solution, and determining whether the polynaphthalene formaldehyde sulfonate sodium salt is contained according to the relative deviation; and S40, quantitative determination: drawing a standard curve according to the concentration and peak area of the series of concentration standard solutions, and calculating the content according to an external standard method. The application realizes accurate qualitative and quantitative determination of the polynaphthalene formaldehyde sulfonate sodium salt by combining chromatographic separation and ultraviolet detection, has the advantages of strong specificity, high accuracy, good reproducibility and simple operation, and is suitable for detection of various pesticide preparations.

The invention discloses a method for detecting lactic acid in displacement liquid, which comprises the following steps: (1) pretreating the displacement liquid, namely pretreating a sample by using an alkaline solution, standing, adding a proper amount of acid solution for neutralization, filtering by using a filter membrane, and taking a subsequent filtrate to obtain a test solution; and (2) detecting the test solution by adopting a high performance liquid chromatography under the chromatographic conditions that a mobile phase is a phosphoric acid aqueous solution, and isocratic elution is carried out. The mobile phase is simple to prepare, various reagents are not needed, the detection cost is saved, the experimental error is reduced, and the lactic acid in the displacement liquid can be accurately analyzed. The phosphoric acid water is used as a mobile phase, the pH value of the phosphoric acid water is about 2, the activity of silicon hydroxyl in the chromatographic column can be just inhibited, long-time use of the chromatographic column is facilitated, the sample peak shape can be improved, and trailing can be reduced.

The invention relates to a quality control method of artemisia apiacea or an artemisia apiacea extract, which comprises the following steps: respectively detecting a test solution and a reference solution by adopting a high performance liquid chromatography, generating a reference fingerprint spectrum by adopting a median method, and calculating the similarity of each common peak; and calculating the content according to the peak areas of artemisinin, artemisinic acid and dihydroartemisinic acid in the chromatograms of the sample to be detected and the reference substance by an external standard method. The mobile phase A is a tetrahydrofuran phosphate aqueous solution, the mobile phase B is acetonitrile, isocratic elution is carried out, and the stationary phase is Agilent Infinity Lab Poroshell 120EC-C18 250mm * 4.6 mm, 2.7 mu m, Caprisil C18 250mm * 4.6 mm, 3.0 mu m or an equivalent column. The method is high in accuracy and good in stability and reproducibility, can be used as a detection method for the fingerprint spectrum of the artemisia apiacea medicinal material, and can be used for simultaneously determining the contents of artemisinin, artemisinic acid and dihydroartemisinic acid.

The invention relates to a method for measuring the concentration of cefadroxil in K2EDTA human plasma through LC-MS / MS. The method comprises the following steps: preparing a standard curve solution containing cefadroxil d4 by taking K2EDTA human plasma as a matrix, preparing a test solution containing cefadroxil, and precipitating the solution by using acetonitrile; determining the concentration of the test solution containing cefadroxil after precipitation by using an internal standard method; a liquid chromatography tandem mass spectrometer is used for determination, a mobile phase of liquid chromatography is composed of a mobile phase A and a mobile phase B, the mobile phase A is an acetic acid aqueous solution, and the mobile phase B is acetonitrile; the elution procedure of the liquid chromatography is isocratic elution, the volume content of acetonitrile in a mobile phase is 10-30%, and the flow velocity of the mobile phase is 0.4-0.8 mL / min. By optimizing chromatographic conditions and mass spectrum parameters, an efficient and accurate cefadroxil concentration detection method is provided, and the accuracy and reliability of data are ensured.

The invention relates to the related technical field of measurement of the content of fluticasone propionate emulsifiable paste, in particular to a method for detecting the content of paste by adopting a high performance liquid chromatography, and particularly relates to a method for measuring the content of fluticasone propionate emulsifiable paste by adopting the high performance liquid chromatography, in particular to a method for detecting the content of fluticasone propionate emulsifiable paste by adopting the high performance liquid chromatography. Comprising the following steps: preparing a mobile phase from a diammonium hydrogen phosphate buffer solution (the pH value is 2.0-5.0), methanol and acetonitrile by adopting an octadecyl silane bonded silica gel column; and carrying out isocratic elution. According to the method disclosed by the invention, the diammonium hydrogen phosphate buffer solution-methanol-acetonitrile with a specific ratio is adopted as a mobile phase, and a chromatographic system in which an octadecyl silane bonded silica gel column and a guard column are connected in series is combined, so that the interference of oily auxiliary materials in a cream matrix is effectively eliminated, and the detection specificity and the separation effect are remarkably improved; besides, key chromatographic parameters such as column temperature, flow velocity and detection wavelength are optimized, and a scientific test sample pretreatment process is matched, so that the accuracy and reliability of a content measurement result are guaranteed.

The invention discloses a method for separating and preparing megastigmatrienone isomers, and belongs to the technical field of isomer separation and preparation. The technical problem to be solved is that in the prior art, mg-level separation preparation of a megastigmatrienone single-configuration isomer cannot be rapidly realized. The key point of the technical scheme is as follows: the method comprises the following steps: (1) mixing megastigmatrienone with methanol to obtain a mixed solution; (2) separating the mixed solution by adopting a reversed-phase preparative chromatography to obtain an isomer of megastigmatrienone; wherein the chromatographic conditions in the reversed-phase preparative chromatography in the step (2) are as follows: a chromatographic column is a reversed-phase semi-preparative chromatographic column, the sample size is 100-800 [mu] L, the flow velocity is 0.1-0.8 mL / min, the detection wavelength is 308 nm, an eluent is methanol-THF-H2O in a ratio of 37.5: 7.5: 55, and isocratic elution is performed.

The invention relates to the technical field of substance detection, and particularly discloses an HPLC-ELCD method for simultaneously detecting calcium gluconate, zinc gluconate and lysine hydrochloride and application. According to the HPLC-ELCD method for simultaneously detecting calcium gluconate, zinc gluconate and lysine hydrochloride, a cation exchange chromatographic column is adopted for isocratic elution of a sample to be detected, and a mobile phase is a methanesulfonic acid-oxalic acid solution; the mobile phase is a methanesulfonic acid-oxalic acid solution, the concentration of methanesulfonic acid is 0.002 mol / L to 0.004 mol / L, and the concentration of oxalic acid is 0.12 g / L to 0.18 g / L. The method disclosed by the invention is high in separation efficiency, good in selectivity, high in detection sensitivity and strong in operability.

The invention relates to the technical field of polypeptide detection, in particular to a method for detecting the content of a semeglutide polymer. A liquid chromatography method is adopted, wherein a chromatographic column is G2000 SWxl; carrying out isocratic elution for 40 minutes; the detection wavelength is 280 nm; the column temperature is 26-30 DEG C; the mobile phase is formed by mixing a phosphate solution of sodium chloride and isopropanol according to the volume ratio of 50: 50. The selected mobile phase has no influence on the retention performance of the chromatographic column, the separation degree of the polymer and the main peak is greater than 1.5, and the requirement on an instrument is lower; accurate and reliable experimental results can be obtained by determining the polymer in the semeglutide through the method.

The invention provides a method for detecting the blood concentration of Shenfukang capsules. The method comprises the following steps: respectively preparing a first detection specimen containing a serum sample and a second detection specimen containing a urine sample; the method comprises the following steps: taking a first sample to be detected and a second sample to be detected, injecting a Supersil ODS 2 chromatographic column, taking 0.1% phosphoric acid aqueous solution-methanol as a mobile phase, respectively detecting the chromatographic peak of calycosin in the first sample to be detected and the chromatographic peak of rhein in the second sample to be detected, and calculating the concentration of calycosin and the concentration of rhein in the samples according to the chromatographic peaks; the chromatographic conditions of the first detection sample are that the detection wavelength is 260 nm, and gradient elution is carried out; the chromatographic conditions of the second detection sample are as follows: the detection wavelength is 254 nm, and isocratic elution is performed. The method has excellent repeatability and high sensitivity, the operation process is simple and easy to implement, the administration scheme can be accurately adjusted and an individualized administration strategy can be implemented in close combination with clinical manifestation and various inspection results of a patient, so that the safety and effectiveness of the medication process are ensured, and the method is suitable for clinical popularization and application. And a reliable medication guarantee is provided for the treatment of patients with chronic kidney diseases.

The application discloses a method for detecting enantiomers in (S)-(1-hydroxypropan-2-yl) tert-butyl carbamate by high performance liquid chromatography. Specifically, the test product is dissolved in a diluent, and detection is performed by using a high performance liquid chromatograph, wherein a CHIRALPAK IG is used as the chromatographic column, the specification of the chromatographic column is an inner diameter of 4.6 mm, a length of 250 mm, and a filler particle size of 5 microns; a mobile phase is water-acetonitrile-tetrahydrofuran, and the volume ratio is 900:75:25; and isocratic elution is adopted. The method is simple and rapid, does not need derivatization treatment, has high sensitivity, good sample solubility, and accurate results, and is suitable for quality control of (S)-(1-hydroxypropan-2-yl) tert-butyl carbamate.

This invention belongs to the field of testing and analysis technology, and discloses a method for rapidly extracting and detecting the content of various terpenoids and sterols in rubber grass. The specific steps are as follows: Step 1: Preparation of standard solutions of terpenoids and sterols. Detection is performed using a general-purpose high-performance liquid chromatograph, eliminating the need for large-scale precision mass spectrometry equipment, resulting in lower purchase and maintenance costs. The operation process is simple and easy to learn, meeting the needs of high-throughput rapid detection of batch samples. A single sample test only takes 35-60 minutes, significantly improving detection efficiency. It can simultaneously complete the qualitative identification and content determination of five target components, including lupeol and taraxasterol. The method has good repeatability and high accuracy, with excellent linearity of the standard curve, and stable and reliable detection results. By optimizing the extraction solvent, temperature, time, and isocratic elution chromatographic conditions, the target components are fully extracted and separated effectively, allowing for accurate acquisition of the content data of each component.

The invention discloses a method for determining isomers in an oseltamivir phosphate product by using high performance liquid chromatography. The chromatographic condition is that a Waters Symmetry C8 chromatographic column is used, a mobile phase is phosphate buffer solution-methanol-acetonitrile, the volume ratio is 620: 245: 135-700: 245: 135, isocratic elution is performed, the concentration of the phosphate buffer solution is 0.040 mol / L to 0.075 mol / L, the pH value is 6.25 to 6.40, the column temperature is 43 to 47 DEG C, the flow velocity is 1.1 to 1.3 ml / min, the detection wavelength is 207 nm, and the sample size is 60 microliters. According to the method, the isomer in the oseltamivir phosphate product can be rapidly and accurately detected, the content of the isomer can be calculated, the operation is simple, the reproducibility is good, the quality control of an oseltamivir phosphate preparation and an intermediate product thereof can be well realized, and a guarantee is provided for synthesis and preparation process optimization.

The invention discloses a separation, analysis and determination method of aztreonam and enantiomers thereof, and belongs to the technical field of pharmaceutical analysis. According to the method, silica gel surface covalently bonded O-9-(tert-butyl carbamoyl) quinine is adopted as a filler (QN-AX, 4.6 mm * 150mm, 5 [mu] m), and methanol / acetonitrile / acetic acid / triethylamine = 50 / 50 / 2 / 1.6 (v / v / v / v) is adopted as a mobile phase for isocratic elution. The method can simply, accurately and efficiently analyze and detect aztreonam and the enantiomer thereof, and effectively control the quality of aztreonam and the enantiomer thereof.

The invention relates to a detection method of a morphonidazole isomer. The method is a high performance liquid chromatography method, a chromatographic column with amylose-tri (3-chloro-5-methyl phenyl carbamate) covalently bonded on the surface of silica gel as a filler is adopted, a mobile phase A is a 0.05 mol / L monopotassium phosphate solution, the pH value is adjusted to 2.0 + / -0.5 with phosphoric acid, a mobile phase B is acetonitrile, and the mobile phase A and the mobile phase B are subjected to isocratic elution according to the volume ratio of (20-30): (80-70). The detection method provided by the invention is simple and convenient to operate, can effectively separate R and S isomers of morphonidazole, and can accurately determine the content of the isomers.

The invention discloses a method for detecting the content of fumaric acid vonoprasan in a fumaric acid vonoprasan tablet, which comprises the following steps: carrying out ultrahigh liquid chromatography detection on a sample to be detected, and determining the content of fumaric acid vonoprasan in the sample to be detected on the basis of a detection result, a mobile phase adopted in the ultrahigh liquid chromatography detection comprises a phosphate buffer solution and methanol, and the volume ratio of the phosphate buffer solution to the methanol is (50-70): (30-50); the elution mode is isocratic elution. According to the detection method, an isocratic elution mode is adopted, the treatment mode is simple, the detection operation time is short, and consumed resources are fewer; the phosphate buffer solution and the methanol in a specific ratio are used as mobile phases, so that the method has the advantages of strong qualitative specificity, accurate quantification, high sensitivity, good method stability, short operation time, high efficiency, rapidness, high detection efficiency and the like, and can realize effective detection of the content of the Vonoprazan fumarate in the Vonoprazan fumarate tablets.

The invention discloses a method for detecting 3-chloroaniline in a trazodone hydrochloride tablet, the detection method is a high performance liquid chromatography, an octadecylsilane bonded silica gel packed column is used, a phosphoric acid aqueous solution with a volume fraction of 0.1 + / -0.02% is used as a mobile phase A, acetonitrile is used as a mobile phase B, gradient elution is carried out, and the elution procedure is as follows: 0-7th minutes, 0-7th minutes, 0-7th minutes, 0-7th minutes, 0-7th minutes, 0-7th minutes, 0-7th minutes, 0-7th minutes, 0-7th minutes, 0-7th minutes and 0-7th the mobile phase A and the mobile phase B with the constant volume fraction are used for isocratic elution, and the volume fraction of the mobile phase B is 2-10%; the volume fraction of the mobile phase B is increased for gradient elution for 12 minutes, and the volume fraction of the mobile phase B is 68%; performing isocratic elution for 17 minutes by keeping the volume fraction of the mobile phase B to be 68%; reducing the volume fraction of the mobile phase B to 5% for gradient elution for 18 minutes; and finally, keeping the volume fraction of the mobile phase B at 5%, performing isocratic elution for 25 minutes, and ending. In the elution process, the total volume fraction of the mobile phase A and the mobile phase B is 100%. Qualitative and quantitative analysis of 3-chloroaniline can be completed only by using high performance liquid chromatography.

The invention discloses a method for determining the dissolution rate and the dissolution curve of purinol tablets. The dissolution rate of the allopurinol tablets is detected by high performance liquid chromatography instead of an ultraviolet spectrophotometer method used at present. According to the method, a chromatographic column is used as a silica gel bonded octadecylsilane column, an acid solution-organic phase is used as a mobile phase, and an isocratic elution mode is adopted for detection. According to the method for detecting the dissolution rate and the dissolution curve of the allopurinol tablet through the high performance liquid chromatography, detection can be easily and conveniently completed, specificity is high, accuracy is high, repeatability is good, application and linearity are achieved, and the dissolution rate of the allopurinol tablet and the dissolution amount of the dissolution curve can be efficiently and rapidly detected.

The invention relates to the technical field of pharmaceutical analysis, and particularly discloses a method for detecting related substances in a key parent nucleus of cefcapene pivoxil hydrochloride. Detection is carried out according to the following high performance liquid chromatography conditions: a chromatographic column is GL Sciences InertSustaiin C18, 4.6 mm * 250 mm, 5 [mu] m, and the column temperature is 25 DEG C; a mobile phase is ammonium acetate solution-acetonitrile-tetrahydrofuran; the detection wavelength is 255 nm to 275 nm, the column temperature is 20 DEG C to 30 DEG C, and isocratic elution is carried out. By adopting the method provided by the invention, various impurities such as D-CPC, 7-ADCA, N-acetyl-D-7-ACA, 7-ACA, D-7-ACA lactone, cephalosporin C and the like in the key mother nucleus of the cefcapene pivoxil hydrochloride are effectively detected, and the condition of the impurities in the key mother nucleus of the cefcapene pivoxil hydrochloride is accurately, qualitatively and quantitatively detected. Therefore, the clinical safety of the cefcapene pivoxil hydrochloride product is improved.