114 results about "Cyanine" patented technology

This invention discloses a near-infrared two-photon dual-mode linked response NTR&Cys fluorescent probe, its preparation method, and its application, belonging to the field of medical detection technology. The structural formula of the near-infrared two-photon dual-mode linked response NTR&Cys fluorescent probe is as follows: The near-infrared two-photon dual-mode linked response NTR&Cys fluorescent probe of this invention possesses a hypoxia dual-mode visual monitoring molecular framework, enabling dual-mode determination of hypoxia microenvironments. It improves the stability of the cyanine near-infrared framework and achieves highly stable and specific detection of nitroreductase under hypoxia. Under hypoxia conditions, the product reacts with NTR, and the single-photon excited near-infrared fluorescence is quenched and then illuminated. Simultaneously, under hypoxia, the probe reacts with Cys to generate a two-photon excited fluorescence signal, effectively avoiding the probe consumption problem of dual-response systems and ensuring the non-interference of fluorescence excitation and emission (dual-mode), thus truly achieving accurate locking of hypoxia microenvironments.

The present application relates to the technical field of fluorescent dyes, in particular to an asymmetric pentamethine cyanine compound. The compound comprises a structure as shown in formula I. The compound provided by the present application has good water solubility, near-infrared absorption, high fluorescence quantum yield and good molar absorption coefficient, and can be used in nucleic acid labeling, DNA sequencing, cell imaging, protein labeling or specific recognition of amino acids.

To provide compounds of the cyanine family having near-infrared emission exhibiting improved physicochemical and biological properties, and to provide conjugates thereof with biological ligands.SOLUTION: Cyanine derivatives of Formula (I) are provided. It may be suitably conjugated to an appropriate targeting moiety via an appropriate functional group which acts as a site of attachment.SELECTED DRAWING: None

The invention relates to a compound as shown in a formula (I) and / or a formula (II) and / or a formula (III) or a tautomer or pharmaceutically acceptable salt thereof, a preparation method and application of the compound or the tautomer or the pharmaceutically acceptable salt in medicine. Specifically, the fluorescent probe can be used as a fluorescent probe for cytological imaging, can also be used as a near-infrared fluorescent imaging probe for diagnosing benign / malignant lesions such as fibrosis, nodules and tumors, and can also be applied to research of related biological events in cytobiology and molecular biology. The fluorescent probe can be particularly applied to fluorescent navigation in tumor resection surgery.

The invention belongs to the technical field of medicines, and discloses a near-infrared fluorescent probe Cy-NEO-Leu for leucine aminopeptidase responsiveness as well as a preparation method and application of the near-infrared fluorescent probe Cy-NEO-Leu. The parent nucleus of the probe is of a heptamethyl cyanine structure, and the probe has high molar absorption coefficient, high fluorescence quantum yield and excellent phototherapy characteristics. The introduction of a neomycin group can increase the water solubility of the probe, and a neomycin multi-ammoniation structure exists in a polycation form under physiological conditions and can target negatively charged cell walls of gram-negative bacteria, so that the probe Cy-NEO-Leu is enriched around the bacteria, can be specifically recognized by leucine aminopeptidase and can be hydrolyzed and activated; and photo-thermal and photodynamic sterilization effects are achieved under laser irradiation. Due to the good biocompatibility, the remarkable active oxygen generation capacity, the excellent photo-thermal conversion efficiency and the unique property of accurately targeting bacteria, the Cy-NEO-Leu is expected to play an important role in a photo-thermal / photodynamic combined treatment scheme, and is used for resisting diseases caused by bacteria and biological membranes and accelerating the healing process of wounds.

The invention relates to a nitroimidazole group meso-substituted heptamethine cyanine dye as well as a preparation method and application thereof. According to the dye, a nitroimidazole group is introduced into the middle position of a heptamethine cyanine parent nucleus, so that the dye has excellent absorption characteristics in a near-infrared region, the excitation wavelength is 730-780 nm, the emission wavelength is 800-860 nm, and the dye has good tissue penetrating power. The nitroimidazole group can effectively quench fluorescence in an excited state through a light-induced electron transfer effect, energy is promoted to be released in a non-radiative transition mode, the photothermal conversion efficiency is remarkably improved, and meanwhile high light stability and low fluorescence quantum yield are kept. The dye can be further prepared into a nano preparation and shows excellent biocompatibility and tumor targeting ability. The compound has wide application prospects in the fields of non-diagnostic or therapeutic protein labeling, bioimaging, photothermal therapy and the like, is especially suitable for preparing efficient photosensitive reagent drugs for tumor photothermal therapy, and provides a new strategy for design of near-infrared photothermal diagnosis and treatment materials.

The application belongs to the technical field of fluorescent probe, and particularly relates to an aminopeptidase fluorescent probe and a preparation method and application thereof. In order to develop an APN near-infrared fluorescent probe suitable for in vivo imaging, a center-position ester group substituted pentamethine cyanine dye platform (Cy5-COOM) is used, and based on an "ester group to carboxyl group" conversion strategy, an aminopeptidase (APN) fluorescent probe Cy5-APN is developed. The probe is firstly hydrolyzed by APN, then a 1,6-elimination reaction occurs, and finally a strong fluorescent center-position carboxyl group substituted pentamethine cyanine dye Cy5-COO is released. In addition, in view of the characteristics that cancer cell surfaces overexpress APN, the probe can distinguish cancer cells / tissues and normal cells / tissues with high contrast, and therefore has great application potential in fluorescence-guided tumor surgery.

The present application relates to fluorescent nanoparticles based on fluorinated zwitterionic random copolymer and its preparation method and application, the fluorescent nanoparticles include the single chain nanoparticles formed by poly trifluoroethyl methacrylate-co-methacryloyloxyethyl sulfobetaine, the molecular chain end of the poly trifluoroethyl methacrylate-co-methacryloyloxyethyl sulfobetaine is connected with ACQ type group selected from anthryl, perylene imide group and pyrene base or AIE type group selected from tetraphenyl ethylene base, carbazole base and cyanine-containing triphenyl ethylene group.The fluorescent nanoparticles based on the poly trifluoroethyl methacrylate-co-methacryloyloxyethyl sulfobetaine of the present application that terminal fluorescent group is terminated, has excellent controllable fluorescent luminescence performance.It is considered that the single chain nanoparticles have isolation effect, avoid the fluorescence quenching effect of ACQ type group, and can provide limited microenvironment for AIE type group, ensure the fluorescent luminescence performance of nanoparticles.

The invention discloses a thiophenol-heptamethine cyanine dye derivative as well as self-assembled nanoparticles, a preparation method and application thereof. The derivative is a thiophenol-heptamethine cyanine dye monomer derivative or a 1, 3, 5-benzene trithiophenol-heptamethine cyanine dye triad derivative; the thiophenol or the 1, 3, 5-benzene trithiophenol is connected with a meso-carbon atom of the heptamethine cyanine dye through a sulfur atom, and the heptamethine cyanine dye is formed by connecting two nitrogen-containing heterocyclic ring units through a conjugated methine chain; the nanoparticles are obtained by dropwise adding a derivative solution into water and then removing an organic solvent. The synthesis method of the thiophenol-cyanine dye derivative is simple and easy to implement, stable nanoparticles can be formed, the tumor targeting property can be improved, the aggregation effect far better than that driven by traditional weak interaction is achieved, synchronous optimization of high tumor accumulation and extremely high heat production efficiency is successfully achieved through self-assembly of nanoparticles, and the thiophenol-cyanine dye derivative has good application prospects. An innovative strategy is provided for the development of a high-performance tumor photo-thermal reagent.

The present disclosure relates in some aspects to methods and systems for analyzing a biological sample comprising contacting the biological sample with a nucleic acid probe non-covalently associated with a monomethine cyanine dye or salt thereof. In some aspects, a signal associated with the monomethine cyanine dye or salt thereof is detected to determine the location of a target analyte in the biological sample.

The application discloses a heavy atom enhanced calix cyanine dye radioactivity detection colorimetric sensor, the dye is prepared by introducing heavy atoms in the calix cyanine dye skeleton, and the radiation sensitivity is significantly improved; the dye reacts with hydroxyl radicals generated by water radiolysis under the action of radiation, conjugate structure is destroyed, and color change from green to yellow is generated; the application comprises a colorimetric test strip prepared from the dye, and a detection system integrated with a smartphone image recognition function, the test strip comprises a base material and an additive; the additive comprises a copolymer, and preparation reaction monomers of the copolymer comprise N,N-diethylaminoethyl acrylate and 3-methylpent-2-ene dimethyl acid; the application has the advantages of high sensitivity, low cost, simple operation, portability and the like, can be used for rapid, visual and quantitative detection of radionuclides in water solution, urine and the like samples, and has important application value in the field of nuclear medicine radiation safety monitoring.

This invention belongs to the field of fluorescent probe technology, specifically relating to a nitroreductase fluorescent probe, its preparation method, and its application. To develop an NTR fluorescent probe with high selectivity, high sensitivity, short response time, and long excitation and emission wavelengths, this invention utilizes pentamethine cyanine dye and a "ester group → carboxyl group" conversion strategy at the central site of this dye to develop a nitroreductase fluorescent probe, Cy5-NTR. In the presence of NADPH, the nitro group of the probe is first catalyzed by the overexpression of NTR by cancer cells, resulting in a single-electron transfer and the generation of a nitro anion radical. This radical is then further reduced to an amino group, followed by a 1,6-rearrangement and elimination reaction, releasing the strongly fluorescent centrally carboxyl-substituted pentamethine cyanine dye Cy5-COO. This probe can not only distinguish cancer cells / tissues from normal cells / tissues with high contrast under hypoxic conditions, but also provide in situ real-time imaging of tumors in tumor-bearing mice.

The invention relates to the technical field of fluorescent dyes, in particular to a synthesis method and application of an asymmetric cyanine dye containing dioxaborinine. The synthesis method comprises the following steps: (1) preparing a compound, the asymmetric cyanine dye disclosed by the invention has remarkable advantages in the aspects of synthesis convenience, active oxygen generation efficiency, treatment mode diversity and detection simplicity, and provides a more efficient and more practical novel photosensitizer choice for tumor photodynamic therapy.

The invention provides a novel class of cyanine dyes that are functionalized with sulfonic acid groups and a linker moiety that facilitates their conjugation to other species and substituent groups which increase the water-solubility, and optimize the optical properties of the dyes. Also provided are conjugates of the dyes, methods of using the dyes and their conjugates and kits including the dyes and their conjugates.

The invention relates to a carbon-carbon double bond connected cyanine dye conjugated organic framework material as well as a preparation method and application thereof, and belongs to the technical field of conjugated polymer materials. The carbon-carbon double bond connected cyanine dye conjugated organic framework material prepared by introducing a cyanine dye into a conjugated organic framework material has excellent optical properties, the absorption spectrum can be expanded to 2000 nm, the material has excellent photo-thermal conversion properties under near-infrared laser irradiation, the ultrahigh temperature of 450 DEG C can be reached, and the stability is good; the preparation method is simple in process and low in cost; and carbon-carbon double bonds can be broken under the action of reductive amine and are degraded into monomers, so that raw materials are recycled.

The invention discloses a mitochondrial targeting pyridinium cyanine photosensitizer compound, a preparation method, a compound and application thereof, and the pyridinium cyanine photosensitizer compound is generated through organic reaction by taking N-methyl-N-hydroxyethyl naphthylamine as an electron donor, pyridinium cyanine as an electron acceptor and double bonds as pi bridge connection. After the pyridycyanine photosensitizer compound is self-assembled and combined with HSA, specific fluorescent staining of mitochondria is achieved, active oxygen is efficiently generated under white light irradiation and directly acts on the mitochondria, and the cancer cell killing efficiency is improved. The preparation method is simple and efficient, and the prepared photosensitizer compound has a good photodynamic therapy effect and biological safety and has a good application prospect.

The invention provides an ultrasonic activated rhenium cyanine dye complex nano preparation, which is characterized in that heptamethine cyanine dye (IR-780) and rhenium (I) tricarbonyl center are coupled through a covalent bond to construct a novel complex Re-Cy7. According to the molecular design, ultrasonic synchronous triggering dual effects are achieved by expanding a pi-conjugated system, oxygen-independent-form. OH is efficiently generated through an I-type mechanism, and a therapeutic dose of CO is accurately released. Furthermore, a nano preparation (Re-Cy7NPs) is constructed through self-assembly of Pluronic F-127, so that the water solubility and the targeted accumulation capacity of an infected part are remarkably improved. The technology breaks through the existing limitation at one stroke: the restriction of an anoxic environment on SDT is overcome, the constraint of light penetration depth on gas release is relieved, efficient synergy of. OH and CO is realized on a single molecule level, and a brand new solution is provided for radically treating MDR infection.

An infrared cut-off filter, including a cyanine dye and a copolymer. The cyanine dye includes tris(pentafluoroethyl) trifluorophosphate and a cation having a polymethine and a nitrogen-containing heterocycle at each end of the polymethine. The copolymer includes a first repeating unit from a first monomer and a second repeating unit from a second monomer different from the first monomer. The first monomer includes a phenolic hydroxyl group.

The invention provides a cyano and carbazolyl-containing triazine compound and application thereof, the cyano and carbazolyl-containing triazine compound has a structure as shown in formula I. The cyano and carbazolyl-containing triazine compound obtained by designing the structure of the compound has excellent performance, and can be used for preparing a novel triazine compound. The organic light-emitting device prepared by using the compound as a light-emitting layer material has relatively low driving voltage and relatively high current efficiency.

This invention discloses a class of enzyme-activated prodrug compounds having the following general structural formula I: This invention discloses a class of enzyme-sensitive chlorambucil analog prodrugs. These prodrugs exhibit high selectivity for hypoxic tumors and can be effectively activated within hypoxic tumor cells. Upon specific activation, the prodrug releases free chemotherapeutic drug chlorambucil, simultaneously releasing the photosensitizer cyanine dye, achieving an "OFF-ON" photosensitivity conversion, and generating a large number of reactive oxygen species, thus achieving the unification and synergy of photodynamic therapy and activatable chemotherapy.

The invention provides a covalent complex of human milk oligosaccharide and a fluorescent reagent and a preparation method of the covalent complex, and belongs to the field of organic chemistry. The application of the human milk oligosaccharide-cyanine dye Cy7 covalent complex provided by the invention in monitoring the metabolic distribution of the human milk oligosaccharide-cyanine dye Cy7 covalent complex in internal tissues and organs through fluorescence imaging has a good tracing effect, and can dynamically trace the metabolic process of human milk oligosaccharide in vivo at different time points; compared with a traditional liquid chromatography-mass spectrometry in-vitro drug metabolism detection method, the fluorescence tracing method has the advantage that the in-vivo metabolism condition of the human milk oligosaccharide can be accurately, intuitively and efficiently monitored.

To provide a novel cyanine compound that more selectively absorbs incident light beyond 800 nm and also excels in light resistance and heat resistance.SOLUTION: Provided is a cyanine compound that is a counterion conjugate comprising an anion and a cation, the anion being represented by formula (I-1). (In formula (I-1), R1 and R2 each independently denote a hydrogen atom or a monovalent organic group; R3 and R4 each independently denote a monovalent group such as a phenyl group; X denotes a hydrogen atom, a halogen atom, or a monovalent organic group; and Y denotes a divalent group such as an n-propenyl group or the like).SELECTED DRAWING: None

The application discloses a kind of offsite aromatic ketone substituted heptamethine cyanine dyes and its synthesis method and application.The offsite aromatic ketone substituted heptamethine cyanine dye is substituted by heptamethine cyanine dye methine chain, changes the electronic cloud arrangement of cyanine dye, so that the absorption and emission wavelength of dye all have blue shift, has good intersystem crossing ability, can quickly pass through cell membrane, can be concentrated in cell mitochondria, can detect physiological process using the change of cell mitochondria, and can produce a large amount of active oxygen after illumination, cause cell death, realize the purpose of photodynamic therapy.The application can be applied to the field of photocatalysis, as near-infrared region luminescent material, biological marker probe, photodynamic therapy, water photolysis hydrogen production and the field of biological sensing.

This invention discloses a near-infrared anthocyanin derivative based on boron cluster modification. The anthocyanin unit in the molecular structure of this derivative serves as a near-infrared fluorescence imaging and photothermal conversion unit, possessing not only near-infrared fluorescence imaging capabilities but also the ability to target tumor cells via an organic anion transport peptide-mediated pathway, enhancing selective accumulation in cancerous tissue. The nested carborane structure in the anthocyanin derivative molecular structure utilizes its strong electron-donating properties to induce photo-induced electron transfer with the anthocyanin unit, significantly improving photothermal conversion efficiency while reducing fluorescence quantum yield. The resulting molecule possesses tumor targeting, near-infrared imaging capabilities, and highly efficient photothermal and boron neutron capture therapy functions, achieving synergistic diagnostic and combined therapeutic effects of boron neutron capture therapy (BNCT) and photothermal therapy (PTT) in a single system.

The application provides a long-absorption-wavelength polymethine cyanine molecule, a preparation method and application, and compound II, compound III and acetate are added into acetic anhydride, stirred at a certain temperature, and treated with an organic solvent to obtain compound I. The application has the beneficial effects that the compound in the application has an absorption wavelength of more than 1200nm and a photo-thermal conversion efficiency of 83.2%.

The application discloses a near-infrared photosensitizer for promoting pyroptosis of endoplasmic reticulum, a preparation process thereof and a tumor photoimmunotherapy effect combining photothermal and photodynamic power. The photosensitizer is modified on a heptamethine cyanine molecule, and a p-toluenesulfonamide group and a tetraphenylethylene (TPE) group are introduced, so that the photosensitizer can specifically recognize sulfamide receptors on an endoplasmic reticulum membrane, is specifically targeted to the endoplasmic reticulum of cells, and the photothermal stability and ROS generation efficiency of the photosensitizer are enhanced. The photosensitizer can introduce different groups or probes to the other end of the TPE, and when R=H, the photosensitizer is IR780T-ER; when R=IR780-ER, the photosensitizer is T780T-ER. The photosensitizer has the effects of endoplasmic reticulum targeting and accumulation in cells, and can cause endoplasmic reticulum stress under near-infrared light irradiation. The photosensitizer can produce immunogenic death by causing endoplasmic reticulum stress of tumor cells, and trigger pyroptosis, so as to enhance the effect of tumor immunotherapy. The photosensitizer disclosed by the application is expected to be applied to photoimmunotherapy of clinical tumors.

The present disclosure provides compositions and methods for the detection, and treatment of cancer. Specifically, the compositions of the present technology include multimodal fluorine-cyanine-DOTA-hapten compositions that may be complexed with a radioisotope (e.g., 175Lu). Also disclosed herein are methods of using the fluorine-cyanine-DOTA-hapten compositions of the present technology in diagnostic imaging as well as pretargeted radioimmunotherapy.

A resin composition for forming a molding member included in a semiconductor package may include an epoxy resin in a range of about 2 wt % to about 10 wt %, a hardener in a range of about 2 wt % to about 10 wt %, a filler in a range of about 70 wt % to about 90 wt %, a light absorber in a range of about 0.1 wt % to about 3 wt %, and an additive in a range of about 0.1 wt % to about 3 wt %, and the light absorber includes a compound having a heptamethine cyanine structure. A method of manufacturing a semiconductor package may include forming a molding member using the resin composition.

The present disclosure provides novel benzothienopyrrole (Bt)-cyanine (Cy) fluorescent compounds, water soluble BtCy fluorescent dyes, water soluble BtCy binding partner conjugates, and tandem dyes thereof. Yellow, orange, red, NIR or IR wavelengths can be used to excite the BtCy fluorescent compounds, conjugates, and tandem dyes. The fluorescent dyes can be conjugated to a binding partner for detecting a target analyte in a biological sample, and are suitable for flow cytometry assays. These dyes have the general formula wherein Ar1 is selected from the group consisting of substituted or unsubstituted benzene, benzene derivatives, monocyclic aryl, polycyclic aryl, monocyclic heteroaryl and polycyclic heteroaryl.

The application discloses a tetrazine cyanine compound and a preparation method and application thereof. The skeleton of the tetrazine cyanine compound is directly connected with a BFI part of cyanine, can better affect the overall fluorescence opening multiple of the tetrazine cyanine compound by adjusting an R1 group, can also realize high-resolution imaging of a lysosome, an endoplasmic reticulum and a mitochondrial membrane by selecting a suitable R1 group and cooperating with a predetermined site molecule, can realize high fluorescence opening multiple in a near-infrared region, can significantly enhance a fluorescence emission signal from an extremely low background level after reacting with BCN, and can also have excellent light stability, wherein the fluorescence intensity of a TBCy3P addition product generated in situ is basically maintained unchanged in the whole irradiation period under continuous 100-frame laser scanning excitation, and the cell morphology is normal.