211 results about "Cyanine" patented technology

The invention belongs to the technical field of medicines, and discloses a near-infrared cyanine probe for detecting penicillin G acylase as well as a preparation method and application of the near-infrared cyanine probe. According to the research, a near-infrared fluorescent probe Cy-NEO-PA responding to penicillin G acylase (PGA) is developed, so that the influence of environmental factors on the formation of an acinetobacter baumannii biological membrane is observed. Research results show that glucose inhibits the generation of PGA to enhance the formation of a biological membrane, while phenylacetic acid (PAA) stimulates the generation of PGA and inhibits the formation of the biological membrane. The observation results highlight the excellent capability of Cy-NEO-PA in accurately measuring PGA kinetics, and reveal the key effect of PGA in biological membrane development. In addition, the Cy-NEO-PA has good biocompatibility, strong active oxygen generation, efficient photo-thermal conversion and bacterial targeting ability, so that the Cy-NEO-PA becomes a promising drug for resisting bacterial infection and promoting wound healing through photo-thermal / photodynamic therapy.

The invention relates to the technical field of analytical chemistry, and particularly discloses a double-signal ratio type up-conversion luminescent probe, a preparation method thereof and application of the double-signal ratio type up-conversion luminescent probe in oxalate detection, and the double-signal ratio type up-conversion luminescent probe comprises up-conversion nanoparticles UCNPs and an iron-chromium cyanine R compound. The upconversion nanoparticles UCNPs are combined with the iron-chromium cyanine R compound to construct a double-emission-peak regulation and control system, when the system is used for oxalate detection, oxalate can be chelated with Fe < 3 + > in the iron-chromium cyanine R compound, blue shift of an induced absorption spectrum is achieved, green and red emission peaks of the UCNPs are synchronously regulated and controlled, and the detection sensitivity is high. The fluorescent-colorimetric bimodal high-sensitivity detection of the oxalate is realized.

The invention belongs to the technical field of near-infrared organic dye synthesis, and particularly discloses a cyanine dye with J aggregation characteristic, J aggregation nanoparticles, and preparation methods and applications of the cyanine dye and the J aggregation nanoparticles. The cyanine dye is 1-ethyl-2-(2-(2-(-3-(2-(1-ethyl-3, 3-dimethyl-1, 3-dihydro-2H-benzo [g] indole-2-subunit) ethylidene)-2-(2, 2, 2-trifluoro-N-(2-methylbenzyl) acetamide) cyclopent-1-ene-1-yl) vinyl)-3, 3-dimethyl-3H-benzo [g] indoleiodised salt, and the structural formula of the cyanine dye is shown in the description. The preparation method is simple, the J aggregation nanoparticles can be prepared by utilizing the unique property of J aggregation, the J aggregation nanoparticles have high light stability and have strong fluorescence emission in a near-infrared second region, and the material prepared by the invention can be used as a contrast agent and is applied to the field of near-infrared second region fluorescence imaging.

The invention discloses a symmetric azaindole-heptamethine cyanine J-aggregate as well as a preparation method and application thereof, and aims to solve the problems of short absorption wavelength, poor stability and the like in the prior art. The cyanine J-aggregate has the maximum absorption wavelength as long as 1035 nm, is located in an NIR-II region and has excellent photo-thermal performance and stability, and the photo-thermal stability is improved by nearly 68% compared with that of an existing J-aggregate. The preparation method comprises multiple steps of reaction, and finally self-assembly is carried out in a PBS solution to form the J-aggregate. The cyanine J-aggregate shows the capabilities of efficiently killing tumor cells and completely ablating tumors in vivo in tumor photothermal therapy, is good in biocompatibility, expands the application scene of diagnosis and treatment integration, and has a wide prospect in the field of tumor photothermal therapy.

This invention discloses a near-infrared two-photon dual-mode linked response NTR&Cys fluorescent probe, its preparation method, and its application, belonging to the field of medical detection technology. The structural formula of the near-infrared two-photon dual-mode linked response NTR&Cys fluorescent probe is as follows: The near-infrared two-photon dual-mode linked response NTR&Cys fluorescent probe of this invention possesses a hypoxia dual-mode visual monitoring molecular framework, enabling dual-mode determination of hypoxia microenvironments. It improves the stability of the cyanine near-infrared framework and achieves highly stable and specific detection of nitroreductase under hypoxia. Under hypoxia conditions, the product reacts with NTR, and the single-photon excited near-infrared fluorescence is quenched and then illuminated. Simultaneously, under hypoxia, the probe reacts with Cys to generate a two-photon excited fluorescence signal, effectively avoiding the probe consumption problem of dual-response systems and ensuring the non-interference of fluorescence excitation and emission (dual-mode), thus truly achieving accurate locking of hypoxia microenvironments.

The invention discloses a multi-cyano offset substituted pentamethine cyanine dye and a synthesis method and application thereof, and the dye has the following general formula structure: # imgabs0. According to the multi-cyano offset substituted pentamethine cyanine dye disclosed by the invention, the absorption emission wavelength is increased by introducing an offset substitution structure; the dye has near-infrared first-area imaging and treatment performance, so that the optimal excitation and emission wavelength can be flexibly selected, the dye shows obvious Stokes shift, reduction of self-absorption and scattering effects is facilitated, the imaging definition and depth are improved, the molar extinction coefficient of the prepared dye is up to 190000, and the dye can be applied to near-infrared first-area imaging and treatment of the near-infrared first-area imaging and treatment of the near-infrared first-area imaging and treatment of the near-infrared first-area imaging. According to the present invention, with the application of the dye, the light energy utilization degree of the dye is substantially improved, the long wavelength light with the deeper penetration depth can be strongly absorbed, the light energy capture and conversion can be effectively achieved, the photosensitizer efficiency can be improved, the light dose can be reduced, the phototoxicity can be reduced, the high absolute fluorescence quantum yield can be represented, and the biological imaging effect can be substantially improved; and the imaging quality is higher.

The invention discloses a fluorine-substituted pentamethine cyanine dye and a preparation method and application thereof, the fluorine-substituted pentamethine cyanine dye has a structure shown in a general formula I: # imgabs0 #, and by introducing fluorine atoms to an indole benzene ring and introducing different groups to an N site, accurate regulation and control of light stability of dye molecules are realized. The fluorine-substituted pentamethine cyanine dye prepared by the invention shows remarkable advantages in spectral characteristics, has a molar extinction coefficient higher than that of the traditional cyanine dye and has strong light energy absorption capacity, and is beneficial to improving the light energy utilization rate and reducing the dye dosage; the absolute fluorescence quantum yield reaches a high level, the intensity and sensitivity of imaging signals are remarkably enhanced, and a more sensitive and reliable tool is provided for biological imaging and molecular detection.

The invention provides a series of cyanine or cyanine-like multifunctional probe molecules which are derivatives with a near-infrared cyanine dye molecular skeleton, and the skeleton is modified with any one or a combination of more than two of a protein affinity agent structure, a targeting group or a functional group; the near-infrared cyanine dye molecule is a near-infrared indole cyanine dye molecule or a near-infrared naphthopyrrole cyanine or flavonoid dye molecule; the structure of the protein affinity agent is from a 4 (p-iodophenyl) butyric acid derivative, Evans blue or a derivative thereof; the targeting group is a structure containing any one pharmaceutically acceptable targeting peptide; the functional group comes from any nuclide chelating agent or a functional drug molecule with a modification site. The multifunctional probe molecule provided by the invention can realize clear near-infrared first-region and second-region lymphatic imaging and angiography, and has good biological safety and adjustable circulation metabolism time at the same time. The invention also provides a method for preparing the multifunctional probe molecule and application of the multifunctional probe molecule in multi-mode diagnosis and treatment.

The present invention discloses an anthraquinone and anthracene compound meso-substituted cyanine dye, its preparation method, and application. The anthraquinone and anthracene compound meso-substituted cyanine dye is prepared by meso-substituting a heptamethine cyanine dye with an anthraquinone or anthracene compound. The substituent groups regulate the fluorescence emission and heat release pathways of the dye, enhancing the photothermal effect of the dye molecule and enabling photoacoustic imaging. Furthermore, its excellent biocompatibility and low cytotoxicity make it suitable for use in the biological and medical fields.

The present application relates to the technical field of fluorescent dyes, in particular to an asymmetric pentamethine cyanine compound. The compound comprises a structure as shown in formula I. The compound provided by the present application has good water solubility, near-infrared absorption, high fluorescence quantum yield and good molar absorption coefficient, and can be used in nucleic acid labeling, DNA sequencing, cell imaging, protein labeling or specific recognition of amino acids.

Disclosed is a compound having the following formula (1):as well as to the use of such as a dye sensitizer.Also disclosed is a dye sensitized solar cell including at least one compound of formula (I) as dye sensitizer.

To provide compounds of the cyanine family having near-infrared emission exhibiting improved physicochemical and biological properties, and to provide conjugates thereof with biological ligands.SOLUTION: Cyanine derivatives of Formula (I) are provided. It may be suitably conjugated to an appropriate targeting moiety via an appropriate functional group which acts as a site of attachment.SELECTED DRAWING: None

The invention relates to a compound as shown in a formula (I) and / or a formula (II) and / or a formula (III) or a tautomer or pharmaceutically acceptable salt thereof, a preparation method and application of the compound or the tautomer or the pharmaceutically acceptable salt in medicine. Specifically, the fluorescent probe can be used as a fluorescent probe for cytological imaging, can also be used as a near-infrared fluorescent imaging probe for diagnosing benign / malignant lesions such as fibrosis, nodules and tumors, and can also be applied to research of related biological events in cytobiology and molecular biology. The fluorescent probe can be particularly applied to fluorescent navigation in tumor resection surgery.

The invention belongs to the technical field of medicines, and discloses a near-infrared fluorescent probe Cy-NEO-Leu for leucine aminopeptidase responsiveness as well as a preparation method and application of the near-infrared fluorescent probe Cy-NEO-Leu. The parent nucleus of the probe is of a heptamethyl cyanine structure, and the probe has high molar absorption coefficient, high fluorescence quantum yield and excellent phototherapy characteristics. The introduction of a neomycin group can increase the water solubility of the probe, and a neomycin multi-ammoniation structure exists in a polycation form under physiological conditions and can target negatively charged cell walls of gram-negative bacteria, so that the probe Cy-NEO-Leu is enriched around the bacteria, can be specifically recognized by leucine aminopeptidase and can be hydrolyzed and activated; and photo-thermal and photodynamic sterilization effects are achieved under laser irradiation. Due to the good biocompatibility, the remarkable active oxygen generation capacity, the excellent photo-thermal conversion efficiency and the unique property of accurately targeting bacteria, the Cy-NEO-Leu is expected to play an important role in a photo-thermal / photodynamic combined treatment scheme, and is used for resisting diseases caused by bacteria and biological membranes and accelerating the healing process of wounds.

The invention relates to a nitroimidazole group meso-substituted heptamethine cyanine dye as well as a preparation method and application thereof. According to the dye, a nitroimidazole group is introduced into the middle position of a heptamethine cyanine parent nucleus, so that the dye has excellent absorption characteristics in a near-infrared region, the excitation wavelength is 730-780 nm, the emission wavelength is 800-860 nm, and the dye has good tissue penetrating power. The nitroimidazole group can effectively quench fluorescence in an excited state through a light-induced electron transfer effect, energy is promoted to be released in a non-radiative transition mode, the photothermal conversion efficiency is remarkably improved, and meanwhile high light stability and low fluorescence quantum yield are kept. The dye can be further prepared into a nano preparation and shows excellent biocompatibility and tumor targeting ability. The compound has wide application prospects in the fields of non-diagnostic or therapeutic protein labeling, bioimaging, photothermal therapy and the like, is especially suitable for preparing efficient photosensitive reagent drugs for tumor photothermal therapy, and provides a new strategy for design of near-infrared photothermal diagnosis and treatment materials.

The application belongs to the technical field of fluorescent probe, and particularly relates to an aminopeptidase fluorescent probe and a preparation method and application thereof. In order to develop an APN near-infrared fluorescent probe suitable for in vivo imaging, a center-position ester group substituted pentamethine cyanine dye platform (Cy5-COOM) is used, and based on an "ester group to carboxyl group" conversion strategy, an aminopeptidase (APN) fluorescent probe Cy5-APN is developed. The probe is firstly hydrolyzed by APN, then a 1,6-elimination reaction occurs, and finally a strong fluorescent center-position carboxyl group substituted pentamethine cyanine dye Cy5-COO is released. In addition, in view of the characteristics that cancer cell surfaces overexpress APN, the probe can distinguish cancer cells / tissues and normal cells / tissues with high contrast, and therefore has great application potential in fluorescence-guided tumor surgery.

The present application relates to fluorescent nanoparticles based on fluorinated zwitterionic random copolymer and its preparation method and application, the fluorescent nanoparticles include the single chain nanoparticles formed by poly trifluoroethyl methacrylate-co-methacryloyloxyethyl sulfobetaine, the molecular chain end of the poly trifluoroethyl methacrylate-co-methacryloyloxyethyl sulfobetaine is connected with ACQ type group selected from anthryl, perylene imide group and pyrene base or AIE type group selected from tetraphenyl ethylene base, carbazole base and cyanine-containing triphenyl ethylene group.The fluorescent nanoparticles based on the poly trifluoroethyl methacrylate-co-methacryloyloxyethyl sulfobetaine of the present application that terminal fluorescent group is terminated, has excellent controllable fluorescent luminescence performance.It is considered that the single chain nanoparticles have isolation effect, avoid the fluorescence quenching effect of ACQ type group, and can provide limited microenvironment for AIE type group, ensure the fluorescent luminescence performance of nanoparticles.

The invention discloses a thiophenol-heptamethine cyanine dye derivative as well as self-assembled nanoparticles, a preparation method and application thereof. The derivative is a thiophenol-heptamethine cyanine dye monomer derivative or a 1, 3, 5-benzene trithiophenol-heptamethine cyanine dye triad derivative; the thiophenol or the 1, 3, 5-benzene trithiophenol is connected with a meso-carbon atom of the heptamethine cyanine dye through a sulfur atom, and the heptamethine cyanine dye is formed by connecting two nitrogen-containing heterocyclic ring units through a conjugated methine chain; the nanoparticles are obtained by dropwise adding a derivative solution into water and then removing an organic solvent. The synthesis method of the thiophenol-cyanine dye derivative is simple and easy to implement, stable nanoparticles can be formed, the tumor targeting property can be improved, the aggregation effect far better than that driven by traditional weak interaction is achieved, synchronous optimization of high tumor accumulation and extremely high heat production efficiency is successfully achieved through self-assembly of nanoparticles, and the thiophenol-cyanine dye derivative has good application prospects. An innovative strategy is provided for the development of a high-performance tumor photo-thermal reagent.

The present disclosure relates in some aspects to methods and systems for analyzing a biological sample comprising contacting the biological sample with a nucleic acid probe non-covalently associated with a monomethine cyanine dye or salt thereof. In some aspects, a signal associated with the monomethine cyanine dye or salt thereof is detected to determine the location of a target analyte in the biological sample.

The application discloses a heavy atom enhanced calix cyanine dye radioactivity detection colorimetric sensor, the dye is prepared by introducing heavy atoms in the calix cyanine dye skeleton, and the radiation sensitivity is significantly improved; the dye reacts with hydroxyl radicals generated by water radiolysis under the action of radiation, conjugate structure is destroyed, and color change from green to yellow is generated; the application comprises a colorimetric test strip prepared from the dye, and a detection system integrated with a smartphone image recognition function, the test strip comprises a base material and an additive; the additive comprises a copolymer, and preparation reaction monomers of the copolymer comprise N,N-diethylaminoethyl acrylate and 3-methylpent-2-ene dimethyl acid; the application has the advantages of high sensitivity, low cost, simple operation, portability and the like, can be used for rapid, visual and quantitative detection of radionuclides in water solution, urine and the like samples, and has important application value in the field of nuclear medicine radiation safety monitoring.

This invention belongs to the field of fluorescent probe technology, specifically relating to a nitroreductase fluorescent probe, its preparation method, and its application. To develop an NTR fluorescent probe with high selectivity, high sensitivity, short response time, and long excitation and emission wavelengths, this invention utilizes pentamethine cyanine dye and a "ester group → carboxyl group" conversion strategy at the central site of this dye to develop a nitroreductase fluorescent probe, Cy5-NTR. In the presence of NADPH, the nitro group of the probe is first catalyzed by the overexpression of NTR by cancer cells, resulting in a single-electron transfer and the generation of a nitro anion radical. This radical is then further reduced to an amino group, followed by a 1,6-rearrangement and elimination reaction, releasing the strongly fluorescent centrally carboxyl-substituted pentamethine cyanine dye Cy5-COO. This probe can not only distinguish cancer cells / tissues from normal cells / tissues with high contrast under hypoxic conditions, but also provide in situ real-time imaging of tumors in tumor-bearing mice.

The invention discloses a multi-rotor near-infrared second-region cyanine molecule as well as a preparation method and application thereof, and belongs to the technical field of organic luminescent materials in the field of organic synthesis. The compound provided by the invention has a structural formula as shown in a structural formula (I): # imgabs0 #. The compound provided by the invention can realize near-infrared second-region tumor fluorescence imaging and high-resolution angiography by utilizing the penetration advantage of a near-infrared second region, and can realize near-infrared second-region photothermal immunotherapy by activating tumor immunogenic cell death.

The invention relates to the technical field of fluorescent dyes, in particular to a synthesis method and application of an asymmetric cyanine dye containing dioxaborinine. The synthesis method comprises the following steps: (1) preparing a compound, the asymmetric cyanine dye disclosed by the invention has remarkable advantages in the aspects of synthesis convenience, active oxygen generation efficiency, treatment mode diversity and detection simplicity, and provides a more efficient and more practical novel photosensitizer choice for tumor photodynamic therapy.

The invention provides a novel class of cyanine dyes that are functionalized with sulfonic acid groups and a linker moiety that facilitates their conjugation to other species and substituent groups which increase the water-solubility, and optimize the optical properties of the dyes. Also provided are conjugates of the dyes, methods of using the dyes and their conjugates and kits including the dyes and their conjugates.

The present application relates to a kind of based on heptamethine cyanine molecule, its preparation method and application, belong to medical technology field.Compounds B1-3, C1 and C3 can specifically examine nitroreductase, be activated by nitroreductase and photoacoustic signal is enhanced.Introducing heavy atom, significantly improve photo-thermal conversion efficiency and intersystem crossing, promote photothermal therapy and photodynamic therapy.Compound C1 shows specific tumor killing effect and obvious photoacoustic signal in vivo by excellent synergistic PTT / PDT effect, provides a general strategy for cancer photoacoustic diagnosis and enhanced phototherapy.Compound C3 realizes staphylococcus aureus in situ pneumonia photoacoustic imaging for the first time, and can detect bacteria in tumor with high sensitivity and specificity, provides a promising new method for early detection of deep bacterial infection in vivo.

The invention relates to a polymethine cyanine photosensitizer compound as shown in a formula I, a preparation method thereof, nano particles prepared from the polymethine cyanine photosensitizer compound and application of the polymethine cyanine photosensitizer compound. The polymethine cyanine photosensitizer compound has an obvious photothermal effect, can generate active oxygen such as superoxide anions (O2 <.->) and the like, and can be used for preparing the nano particles. The compound can be used for preparing a high-efficiency photosensitizer for tumor photothermal / photodynamic synergistic treatment and a photoacoustic reagent for photoacoustic diagnosis; the synthesis method disclosed by the invention is relatively simple, convenient to operate and suitable for mass production. # imgabs0 #

The present invention relates to a fluorescent nanoparticle, a fluorescent probe, a preparation method and an application thereof. The fluorescent nanoparticle is an SrS nanoparticle doped with Pr, Sm and Mn. The fluorescent probe comprises mesoporous silica loaded with the fluorescent nanoparticle, a DNAzyme complex coupled to the surface of the mesoporous silica, and a shell located on the outer layer of the DNAzyme complex. In the presence of near-infrared light and a specific nucleic acid fragment, the present invention activates the fluorescent probe system and releases the cyanine dye FD-1080 in the DNAzyme complex structure, and monitors the near-infrared second-region fluorescence emission of FD-1080 to realize the analysis of the target fragment. The present invention not only broadens the selectable fluorescent nanomaterials for biological imaging, but also contributes to the development of near-infrared light-controlled biochemical sensors and near-infrared light-controlled drug delivery carriers, and promotes the application of fluorescence analysis technology and light-controlled nanomachines in the field of biomedical detection.

The invention relates to a carbon-carbon double bond connected cyanine dye conjugated organic framework material as well as a preparation method and application thereof, and belongs to the technical field of conjugated polymer materials. The carbon-carbon double bond connected cyanine dye conjugated organic framework material prepared by introducing a cyanine dye into a conjugated organic framework material has excellent optical properties, the absorption spectrum can be expanded to 2000 nm, the material has excellent photo-thermal conversion properties under near-infrared laser irradiation, the ultrahigh temperature of 450 DEG C can be reached, and the stability is good; the preparation method is simple in process and low in cost; and carbon-carbon double bonds can be broken under the action of reductive amine and are degraded into monomers, so that raw materials are recycled.

The invention discloses a mitochondrial targeting pyridinium cyanine photosensitizer compound, a preparation method, a compound and application thereof, and the pyridinium cyanine photosensitizer compound is generated through organic reaction by taking N-methyl-N-hydroxyethyl naphthylamine as an electron donor, pyridinium cyanine as an electron acceptor and double bonds as pi bridge connection. After the pyridycyanine photosensitizer compound is self-assembled and combined with HSA, specific fluorescent staining of mitochondria is achieved, active oxygen is efficiently generated under white light irradiation and directly acts on the mitochondria, and the cancer cell killing efficiency is improved. The preparation method is simple and efficient, and the prepared photosensitizer compound has a good photodynamic therapy effect and biological safety and has a good application prospect.

Provided is an image sensor including a substrate including a first surface and a second surface opposite to the first surface, and a near-infrared light-blocking filter disposed on the second surface of the substrate. The substrate includes a plurality of pixels, the plurality of pixels each includes a photoelectric conversion region, the near-infrared light-blocking filter includes a mixture of a first cyanine dye, a second cyanine dye, and a third cyanine dye, the first cyanine dye is represented by General Formula 1, the second cyanine dye is represented by General Formula 2, and the third cyanine dye is represented by General Formula 3 or General Formula 4.