265 results about "Fluorochrome Dye" patented technology

The invention belongs to the technical field of plant fungus molecular biology detection, and discloses an LAMP primer group, a kit and a detection method for detecting oat smut pathogenic bacteria. A group of LAMP (loop-mediated isothermal amplification) specific primers are designed according to a specific sequence on a whole genome of the oat smut pathogenic bacteria Ustilago hordei, results are judged through a real-time fluorescence quantification method, an agarose gel electrophoresis method and an SYBR Green I fluorescent dye developing method, and the oat smut pathogenic bacteria carried by oat seeds are detected. The LAMP detection method for the oat smut pathogenic bacteria, established by the invention, is strong in specificity, high in sensitivity, high in speed and low in cost, provides a new technical means for detection of the oat smut pathogenic bacteria carried by the oat seeds, and has relatively high practical application value.

In one embodiment, a method is disclosed to determine one or more biological cells of interest to identify and count in a mixed biological sample fluid with differing biological cells. The method can include selecting cell markers associated for biological cells to which conjugated antibodies can attach with differing fluorescent dyes; displaying a panel builder graphical user interface window to display a co-expression matrix by biological cell type to assist in selecting cell markers to assign co-expression; and selecting cell markers to assign co-expression with an input device.

Compositions, testing chambers and methods for testing a fuel sample for microbial contamination (including fuels treated with a biocide) are provided, which comprise: a quantity of hydrocarbon fuel; a microbial contamination wherein the microbial contamination further comprises nucleic acid in the form of both DNA, RNA or a combination thereof, and an analyzing solution; wherein the analyzing solution comprises at least six (6) primer pairs for amplification of at least one target locus, wherein at least one primer of each pair of primers is labeled with a fluorescent dye and wherein at least one of the primer pair binds to the nucleic acid of the microbial contamination.

A method of medical multi-dye fluorescence imaging. The method including: capturing fluorescent images of an operating field in which two or more different fluorescent dyes are present in successive sequences of images with a surgical or diagnostic imaging device, each sequence of images alternating through different modes of excitation lighting adapted for at least one each of two or more of the two or more different dyes, and processing the captured images of each successive sequence of images in combination with each other. The processing comprising determining the respective local distributions of the two or more different fluorescence dyes causing the observed different distributions of brightness in the two or more different fluorescent images of the sequence and producing one or more images displaying the determined local distributions of the two or more different fluorescent dyes separately.

In one embodiment, a method of building an optimized color flow cytometry panel is disclosed using a full spectrum flow cytometer with five excitation lasers and five corresponding detection modules. In another embodiment, a graphical user interface is disclosed generated by a server computer from a fluorochrome database and displayed by a client computer to assist in the selection of a set of fluorochromes for use in an assay to analyze biological samples. The GUI can display spectra graphs to visually show how fluorochromes may overlap and can generate similarity indexes for the paired fluorochrome interference and a complexity index for overall many to many interferences generated by a selected group or set of fluorochromes.

The present application relates to the technical field of molecular biology detection, in particular to an absolute antibody Taq DNA polymerase activity determination method based on real-time fluorescence quantitative detection, comprising the following steps: firstly, establishing a standard curve of DNA content and fluorescence value by reacting different concentrations of double-stranded lambda DNA with fluorescent dyes; then, performing an extension reaction in a specific reaction system using M13 single-stranded DNA as a template, recording fluorescence values in real time, calculating net fluorescence values and establishing a linear relationship between the net fluorescence values and reaction time; converting the amount of newly generated double-stranded DNA at different time points by using the standard curve, further calculating the consumption amount of dNTPs, and finally obtaining the absolute activity of the antibody Taq DNA polymerase according to the definition of the activity of the antibody Taq DNA polymerase. The whole process combines fluorescence detection and a standard curve to realize accurate determination of enzyme activity.

The invention relates to the technical field of sensing of magnetic nano photoelectric materials, in particular to a responsive microsphere based on a three-dimensional magnetic photonic crystal and a preparation method and application thereof, and the responsive microsphere is formed by arranging polyhydroxy compound modified magnetic nanoparticles in a spherical polymer into a non-close packing face-centered cubic or body-centered cubic structure. The responsive microsphere based on the three-dimensional magnetic photonic crystal is easy in color distinguishing, has no angle dependence, does not need to depend on a fluorescent dye or a radioactive label, can realize real-time and in-situ monitoring through own optical characteristics, breaks through the bottlenecks of sensitivity and resolution of a traditional sensor, realizes visual detection, simplifies the detection process, and is suitable for mass production. The cost is reduced. According to the responsive microsphere based on the three-dimensional magnetic photonic crystal, the substance diffusion path is shortened through the small size of the responsive microsphere, and the response speed is remarkably increased in combination with the high specific surface area.

The invention belongs to the field of biosensing and microbiological detection, and relates to a staphylococcus aureus rapid counting method based on fluorescence confinement discretization. According to the method, oat bran is used as a natural carbon source, lysozyme active fragments are used as targeting ligands, targeting carbon quantum dots are synthesized through a one-step hydrothermal method, the targeting carbon quantum dots are modified by fluorescent dye SRB to obtain luminescence enhanced carbon quantum dots, and the luminescence enhanced carbon quantum dots can be specifically combined with cell walls of staphylococcus aureus, so that the staphylococcus aureus has stable fluorescence signals. And then, by taking the marked thalli as a core layer and a high-molecular polymer as a shell layer, preparing a core-shell nanofiber membrane by adopting a coaxial electrostatic spinning technology to realize spatial confinement and monomer discretization of the thalli, so that a group fluorescence signal is converted into a single-thalli distinguishable signal, and rapid and sensitive quantitative detection of the staphylococcus aureus is realized. The method is simple, convenient, high in sensitivity, suitable for rapid visualization and quantitative detection of staphylococcus aureus in foods such as dairy products, meat products and beverages, and wide in application prospect.

This invention provides a medical marker that offers excellent mounting stability, improved retention period, and superior visibility from the outside of tubular organs. [Solution] The medical marker 100 according to the present invention is a medical marker 100 that is implanted in a tubular organ in the body, and comprises a main body portion 120 extending in the longitudinal direction L, and two or more locking portions 150 that extend from a proximal end portion 151 fixed to a part of the distal side of the main body portion 120 to a tip portion 154. The locking portions 150 are configured to be deformable into a curved state in which they are curved laterally from the proximal end portion 151 so that the tip portion 154 is positioned proximal to the proximal end portion 151, and an extended state in which they extend in the longitudinal direction L from the proximal end portion 151. The main body portion 120 or the locking portions 150 are arranged with a phosphor containing a fluorescent dye that emits fluorescence in a predetermined wavelength range when irradiated with excitation light.

The organic electroluminescent device includes a first electrode, a second electrode and an organic layer located between the first electrode and the second electrode, the organic layer includes a light-emitting layer, the light-emitting layer includes a host material, a thermally activated delayed fluorescence sensitizer and a green fluorescent dye, the green fluorescent dye includes a structure as shown in formula I. A thermally activated sensitized fluorescence technique is used, and the green fluorescent dye of a specific structure in combination with the sensitizer and the host material is used, so as to achieve the effects of narrowing the spectrum of a device and improving the green color purity. The efficiency of the organic electroluminescent device is equivalent to that of a phosphorescent green light device, so that a display panel including the organic electroluminescent device has a large display color gamut area.

The present disclosure provides improved and useful techniques for cross-standardization of flow cytometry instruments and, particularly, spectral flow cytometry instruments. Aspects of the disclosure include methods of calibrating a flow cytometer having a plurality of fluorescence channels. Methods of interest utilize calibration sets of bead populations, wherein each bead population of the calibration set includes a different fluorophore attached to a surface thereof and the calibration set includes a number of bead populations that is less than the number of fluorescence channels of the flow cytometer. Flow cytometers, non-transitory computer-readable storage media, and kits including, e.g., calibration sets of bead populations for carrying out the subject methods are also provided.

The present application relates to the technical field of fluorescent dyes, in particular to an asymmetric pentamethine cyanine compound. The compound comprises a structure as shown in formula I. The compound provided by the present application has good water solubility, near-infrared absorption, high fluorescence quantum yield and good molar absorption coefficient, and can be used in nucleic acid labeling, DNA sequencing, cell imaging, protein labeling or specific recognition of amino acids.

Disclosed herein are viewing systems configured for viewing an image of a biological object illuminated with light and a viewing device (e.g., loupes, glasses, etc.) and methods for operation thereof. The viewing device comprises a plurality of viewing modes: a white light mode, a fluorescent light mode, and an overlay mode, and a shutter that opens and closes in accordance with the viewing mode. An overlay image is an image of the biological object when the biological object is illuminated with both white light and fluorescent light, causing the overlay image to be an overlay of the white light image and the fluorescent light image. The disclosed systems and methods allow a user (e.g., a surgeon, medical staff, etc.) to preserve the ergonomics offered by eyewear while providing fluorescent viewing capabilities, particularly with visible fluorescent dyes.

Providing control over the nucleic acid amplification process. [Solution] Methods, systems, reaction mixtures, kits, apparatus, and systems for monitoring nucleic acid amplification reaction mixtures for abnormalities. Deviating temperatures and buffer compositions from normal or expected conditions may occur during the preparation of the reaction mixture, and / or may be caused by errors in the functionality of the nucleic acid amplification system. An exemplary method includes determining fluorescence-based values ​​from fluorophore-containing nucleic acid amplification reaction mixtures and determining whether those values ​​meet predetermined values ​​or indicate abnormalities.

The application provides a visual micro-nano optical waveguide refractive index sensing system, and a hardware part comprises a nano fiber taper coupled fluorescent doped polymer microwire; the nano fiber taper coupled fluorescent doped polymer microwire is coupled by a nano fiber taper and a fluorescent doped polymer microwaveguide; the nano fiber taper is obtained by adopting a flame tapering method from a standard single-mode optical fiber; the fluorescent doped polymer microwaveguide is directly prepared by wire drawing from a mixed solution doped with a fluorescent dye; the nano fiber taper and the fluorescent doped polymer microwaveguide placed in a channel of an optical substrate jointly form an evanescent wave coupling structure; a signal analysis part enhances, edge extracts, smoothes and peak extracts a fluorescent image of the microwire through an image analysis algorithm, obtains an oscillation period of a fluorescent light path in the microwire under a current environment, establishes a response relationship between the light path period and the environmental refractive index, realizes direct real-time obtaining of environmental change conditions from optical images of a sensing unit shot by a camera, and thus obtains the change of the refractive index.

Herein is reported a method for determining non-specific clearance of an antibody comprising the steps of incubating the antibody conjugated to a pH-sensitive fluorescent dye with a primary human endothelial cell, and determining the fluorescence intensity of the primary human endothelial cell, wherein an increase in the fluorescence intensity of the primary human endothelial cell relative to a background level is indicative of non-specific clearance of the antibody.

The application provides a method for measuring and calculating the grade of silver minerals above 0.037 mm, and belongs to the technical field of mineral grade detection. The application measures and calculates the silver grade in the sample through screening; measures and calculates the content of silver monomer and silver minerals by leaching, and obtains heavy sand products by gravity separation; the heavy sand products are dyed by fluorescence, and monomer silver minerals are selected; the color of the silver minerals is analyzed by scanning electron microscope point scanning data, and the volume of the monomer silver minerals is obtained by melting, so that the content is calculated; the grade of silver minerals above 0.037 mm is obtained by conversion. The application adopts density medium gravity separation and fluorescent dye tracing to quickly and accurately obtain the target minerals; the actual density of the silver minerals is measured and calculated by introducing a scanning electron microscope instead of the theoretical density, the silver minerals are smelted in a mold, the volume is measured and calculated, and the real metal amount of the silver minerals is obtained, which is closer to the actual value; the calculation error caused by impurities and irregular shape of the mineral particles is reduced; and the calculated grade of the silver minerals is more accurate.

Methods of analyzing a fecal sample containing parasitic material and non-parasitic material are disclosed. The method may include combining the fecal sample with a flotation medium in a container. The method may further include at least partially separating the parasitic material and non-parasitic material in the flotation medium. The method may further include extracting at least a portion of the parasitic material from the flotation medium. The method may further include staining or labeling the extracted parasitic material with a fluorochrome comprising a functional group reactive with a functional group of the parasitic material, where the fluorochrome may bind to the parasitic material via a covalent bond. The method may further include placing the labeled parasitic material within an imaging unit for imaging and analysis of the labeled parasitic material.

The application discloses a kind of two-photon fluorescent probe for detecting industrial hydrogen sulfide or sodium sulfide and a preparation method thereof, and relates to the technical field of fluorescent probe.The application designs and synthesizes a compound with D-Π-A structure, which can be used as a two-photon fluorescent probe to selectively detect H2S, and is suitable for detecting H2S or Na2S residue in industry, with high sensitivity and good specificity.The reaction product of the compound and H2S can be used as an organic fluorescent dye.

The invention belongs to the technical field of biosensing detection, and particularly discloses a label-free ratio fluorescence sensor and application thereof in quinine detection. The label-free ratio fluorescent aptamer sensor takes isothiocyanate as an internal reference signal and a fluorescent dye as a detection signal, and is obtained by mixing and incubating a quinine specific nucleic acid aptamer, a triphenylmethane fluorescent dye and isothiocyanate in a buffer solution. According to the invention, isothiocyanate is introduced as an internal reference signal, triphenylmethane dye is selected as a target response fluorescence signal molecule, and the quinine is detected by using the obvious fluorescence change of the target response fluorescence signal molecule in the binding-dissociation process of the target response fluorescence signal molecule and a quinine specific nucleic acid aptamer. The label-free ratio fluorescence sensor has excellent selectivity and stability, can be widely applied to the fields of food safety supervision, drug quality control, environmental water quality detection and the like, and is particularly suitable for rapid detection and emergency monitoring of quinine under the condition of limited resources.

Techniques for measuring single synaptic signals under varying experimental conditions include controlling a video recording microscope to capture, at multiple different times, a first imaged area in a sample holder as a video frame when the sample holder is disposed on a stage and holds a sample of neuronal tissue combined with at least one fluorophore that emits a corresponding electromagnetic wavelength in a synapse during synaptic activity. At least one synaptic region of interest is determined based on a group of pixels in the first imaged area that record electromagnetic emissions from the fluorophore at the different times. An ordered time series of emission intensity is recorded in each of the synaptic region of interest. Peak emission intensity values in the time series are corrected for transmitter label transients. The ordered time series with corrected peak emission intensity values are stored in a data structure with a standard format.

A method is proposed for detecting at least one fluorescence pattern on an immunofluorescence image of a biological cell substrate, comprising the following steps: incubating the cell substrate with a liquid patient sample, which potentially includes primary antibodies, and furthermore with secondary antibodies, which are marked using a fluorescence stain, irradiating the cell substrate using excitation radiation and capturing the immunofluorescence image, determining segmentation information comprising at least one first and one second segmentation area, wherein the segmentation areas each represent a respective cell substrate area, via segmentation of the immunofluorescence image using a first neural network, determining a boundary area, which represents a transition from the first cell substrate area towards the second cell substrate area in the fluorescence image, on the basis of the segmentation information, selecting multiple partial images from the immunofluorescence image along the boundary area, determining a confidence measure of a presence of the fluorescence pattern on the basis of the multiple partial images via a second neural network.

A device for unmixing images of samples with fluorescent dyes includes one or more hardware processors. The device is configured to obtain a mixed image of a sample; determine an unmixed image based on the mixed image; determine a noise-map image based on the mixed image; determine a signal-to-noise image based on the unmixed image and the noise map-image; determine a denoised signal-to-noise image based on the signal-to-noise image; and determine a noise-reduced unmixed image of the sample based on the denoised signal-to-noise image and on the noise-map image.

The invention relates to a diagnostic method for determining the oil quality of an engine oil for an internal combustion engine (2) of a vehicle, wherein the engine oil is or is made with a fluorescent dye, and wherein the internal combustion engine (2) is located in an engine oil circuit (1) in which a detector (6), in particular an LED-based fluorescence sensor, is arranged for detecting a fluorescence intensity, comprising the steps: - if an engine oil change has been detected, an oil quality test routine is started in which the fluorescence intensity is recorded with the detector (6) and stored as the initial intensity value, - repeated, in particular continuous or regular, measurement of the fluorescence intensity of the engine oil as the current intensity value, and - if a determined current intensity value is less than or equal to approximately 10 percent of the initial intensity value, a message is issued indicating that an engine oil change is required.

The invention discloses a drug loading capacity detection method for drug loading vesicles, and belongs to the technical field of biomedicine.The method comprises the steps that Fe3O4 (at) S < O2 > composite magnetic beads with the surfaces modified with phosphatidyl ethanolamine derivatives serve as enrichment carriers, a Cy5 fluorescent dye and a Cu-MOF nano-enzyme-drug aptamer probe are used for constructing a double-signal detection system, and the detection system is used for detecting the drug loading capacity of the drug loading vesicles; quantification is realized through magnetic bead affinity separation, double-signal labeling, catalytic color development, fluorescence correction and drug loading capacity conversion, and the method comprises the following specific steps: preparing affinity magnetic beads and double-signal probes, preparing blank and standard drug loading vesicles, and verifying the integrity; mixing a drug-loaded vesicle sample with the affinity magnetic beads, incubating, performing magnetic separation and washing, and sequentially performing double-signal labeling and purification; tMB-H2O2 substrate is added for reaction, the absorbance of 450 nm and the fluorescence intensity of 670 nm are measured, after blank correction, the three-dimensional regression equation is introduced to calculate the drug concentration, the drug loading capacity is obtained by combining the concentration of the vesicles, the completeness and purity of the vesicles can be synchronously verified, the anti-interference capacity is high, and the method is suitable for detection of drug loading vesicles of different sources.

The embodiment of the present disclosure discloses a kind of azaxanthene fluorescent dyes and preparation method thereof. Among them, azaxanthene fluorescent dyes have the structure shown in general formula (a);The preparation method of azaxanthene fluorescent dyes includes: halogenated benzoic acid and aniline derivative reaction, generate diphenylamine derivative;Diphenylamine derivative is dehydrated and cyclized to generate azaxanthone derivative;The amino group of the azaxanthone derivative is methylated to generate the first nitrogen methyl anthrone derivative;The first nitrogen methyl anthrone derivative is removed from the first protecting group to generate the second nitrogen methyl anthrone derivative;The phenolic hydroxyl group of the second nitrogen methyl anthrone derivative is protected by the second protecting group to obtain the third nitrogen methyl anthrone derivative;The third nitrogen methyl anthrone derivative is reacted with grignard reagent, and the second protecting group is removed under acidic conditions to obtain azaxanthene fluorescent dye.The azaxanthene fluorescent dye can have high luminous efficiency under acidic conditions.

This invention relates to a lysosome-targeting fluorescent dye based on the BODIPY structure and its synthesis method, belonging to the field of organic fluorescent dyes. The invention designs and synthesizes the lysosome-targeting fluorescent dye BDP. The dye molecule uses BODIPY as the parent structure, and a novel lysosome-targeting fluorescent dye molecule is synthesized by introducing diphenylphosphine salt and tert-butyloxycarbonyl groups. The synthetic route of this dye is simple, it possesses excellent photostability, and exhibits good resistance to interference from ions, amino acids, and viscosity. Furthermore, this dye demonstrates superior fluorescence performance in a variety of common solvents, with fluorescence quantum yields exceeding 0.70. Experimental results show that this fluorescent dye can precisely target lysosomes in living cells, demonstrating promising applications in the field of bioimaging.

The invention belongs to the technical field of fluorescent dyes, and particularly relates to a phenanthroline-based double-BODIPY fluorescent dye with an asymmetric structure as well as a preparation method and application of the phenanthroline-based double-BODIPY fluorescent dye. The preparation method comprises the following steps: mixing a chlorinated BODIPY derivative with 1, 10-phenanthroline-5-amino, adding palladium acetate, tri-tert-butylphosphine, potassium tert-butoxide and toluene, and carrying out a heating reflux reaction; and after the reaction is finished, cooling, reducing pressure to remove toluene, dissolving the obtained product with dichloromethane, washing, drying, concentrating, and separating and purifying through silica gel column chromatography to obtain the target dye. The unique asymmetric structure in the prepared target dye destroys the planarity and symmetry of molecules, effectively weakens the fluorescence quenching effect caused by molecular self-absorption and pi... pi accumulation, and further has relatively large Stokes shift and high solid-state luminous efficiency; good application potentials are shown in a plurality of fields such as high-performance solid-state light-emitting devices and biological probes.

Compounds useful as fluorescent or colored dyes are disclosed. The compounds have the following structure (I):or a stereoisomer, tautomer or salt thereof, wherein R1, R2, R3, R4, R5, L1, L2, L3, L4, M, m and n are as defined herein. Methods associated with preparation and use of such compounds are also provided.

The application provides a water-soluble single benzene ring fluorescent dye and a preparation method and application thereof. The single benzene ring fluorescent dye has a structure shown in formula I, and the preparation method comprises the following steps: adding tetrafluoro-p-xylylene cyanide into an organic solvent, stirring and dissolving, adding an alkali, and performing reaction to obtain the water-soluble single benzene ring fluorescent dye. The organic small molecule fluorescent dye has excellent water solubility, and fluorescence quenching does not occur in water. The fluorescent dye has a rigid conjugated structure, the fluorescent dye 1 has a quantum yield of 0.83 in an aqueous solution, and the fluorescent dye 2 can reach a quantum yield of 0.97 in an aqueous solution. In addition, the fluorescent dye has excellent thermal stability and light stability, provides a possibility for further detection, and has a wide application prospect.