422 results about "Fluorochrome Dye" patented technology

There is proposed a method for detecting a presence of a fluorescence pattern type on an organ segment via immunofluorescence microscopy and digital image processing. The steps comprise: provision of the organ segment, incubation of the organ segment with a liquid patient sample, incubation of the organ segment with secondary antibodies which have been labelled with a fluorescent dye, acquisition of a fluorescence image of the organ segment in a colour channel corresponding to the fluorescent dye, and provision of the fluorescence image to a neural network. What takes place by means of the neural network is simultaneous determination of segmentation information through segmentation of the fluorescence image and, furthermore, of a measure of confidence indicating an actual presence of the fluorescence pattern type. What further takes place is determination, on the basis of the previously determined segmentation information, of at least one sub-area of the fluorescence image that is relevant to formation of the fluorescence pattern type, determination, on the basis of the previously determined at least one sub-area, of validity information indicating a degree of a validity of the measure of confidence, and output of the measure of confidence depending on the validity information.

The invention discloses an alanine aminotransferase detection device and method based on a liquid crystal microcavity array, and belongs to the field of optical sensing. The device comprises a functional liquid crystal microcavity array, an excitation optical fiber and a collection optical fiber, the microcavity array is composed of liquid crystal microcavities doped with fluorescent dye and stearic acid, and the multiple microcavities are independently distributed and serve as optical sensing units; the excitation optical fiber is used for synchronously exciting the microcavity to generate an echo wall mode, and the acquisition optical fiber acquires resonance spectrum signals of each channel in real time; the functionalized liquid crystal microcavity is converted into a bipolar configuration from a radial configuration in an enzymatic reaction, echo wall mode spectrum red shift is caused, spectrum shift time is in negative correlation with enzyme concentration, and dual-channel detection is realized by matching with conversion time of a polarized light pattern. According to the detection structure based on the liquid crystal microcavity array provided by the invention, high-flux, real-time and label-free rapid detection on the concentration of alanine aminotransferase can be realized.

Disclosed in the present invention is a stimulated emission depletion and super-resolution fluorescence lifetime imaging method, which is applied to the technical field of optical microscope imaging. The method provided in the present invention combines the stimulated emission depletion and fluorescence lifetime imaging principles, a depletion laser pulse frequency used therein is half of an excitation light pulse frequency, and a time interval between two pulse sequences is adjusted, such that a depletion laser pulse follows an adjacent excitation light pulse; and analysis processing is performed on collected fluorescence lifetime data, such that a super-resolution fluorescence lifetime image is obtained, thereby solving the problem of the fluorescence lifetime of a stimulated emission depletion and fluorescence lifetime imaging microscope being significantly shortened under the action of a high-energy depletion laser, and thus accurately reflecting real information of a fluorochrome and a micro-environment. Therefore, the innovative technique provides important technical support for life science research, significantly improves the accuracy and effectiveness of research, and promotes further development in the field of biomedicine.

The invention provides a method for measuring and calculating the article position of continuous silver ore with the particle size of 0.037 mm or above, and belongs to the technical field of mineral grade detection. The silver grade in the sample is measured and calculated through screening; leaching and measuring the content of silver monomers and continuous silver minerals, and reselecting to obtain a heavy sand product; carrying out fluorescent dyeing on the heavy sand product, and selecting monomer silver minerals; analyzing the color of the silver mineral through point scanning data of a scanning electron microscope, melting the monomer silver mineral to obtain the volume of the monomer silver mineral, and calculating the content of the monomer silver mineral; and carrying out conversion to obtain the continuous silver ore article position with the size of more than 0.037 mm. According to the method, the target mineral is rapidly and accurately obtained by adopting density medium reselection and fluorescent dye tracing; introducing a scanning electron microscope to measure and calculate the actual density of the silver mineral to replace the theoretical density, smelting the silver mineral in a mold, and carrying out volume measurement and calculation to obtain the actual metal amount closer to the actual silver mineral; measuring and calculating errors caused by impurities and irregular shapes of mineral particles are reduced; and the calculated grade of the silver mineral is more accurate.

The invention belongs to the technical field of plant fungus molecular biology detection, and discloses an LAMP primer group, a kit and a detection method for detecting oat smut pathogenic bacteria. A group of LAMP (loop-mediated isothermal amplification) specific primers are designed according to a specific sequence on a whole genome of the oat smut pathogenic bacteria Ustilago hordei, results are judged through a real-time fluorescence quantification method, an agarose gel electrophoresis method and an SYBR Green I fluorescent dye developing method, and the oat smut pathogenic bacteria carried by oat seeds are detected. The LAMP detection method for the oat smut pathogenic bacteria, established by the invention, is strong in specificity, high in sensitivity, high in speed and low in cost, provides a new technical means for detection of the oat smut pathogenic bacteria carried by the oat seeds, and has relatively high practical application value.

An optical sensor patch for measuring water activity is disclosed. The optical sensor patch includes a sheet configured to be in equilibrium with water activity in an environment of the optical sensor patch. A pair of distinct fluorescent dyes are dispersed within the sheet. The fluorescence spectra of the optical sensor patch is dependent on the water activity in the environment of the optical sensor patch. An optical implant and systems and methods for using the optical sensor patch are also disclosed.

The preparation method comprises the following steps: carrying out reflux reaction on 4-bromo-1, 8-naphthalic anhydride and primary amine in an organic solvent to obtain an intermediate product, and carrying out C-N coupling reaction on the intermediate product, a compound containing a nitrogen secondary amine electron-donating group, alkali and a catalyst in the organic solvent to obtain the double-state emission fluorescent dye. The two-state emission dye is obtained. An amino auxochrome group of a bridge ring structure is modified in a 4-bromine-1, 8-naphthalimide parent, the fluorescence quantum yield of the dye in a polar solvent is improved through the characteristic that nitrogen atom lone pair electrons of the nitrogen-containing bridge ring structure are not easy to overturn, and pi-pi stacking, which is not beneficial to emission, of the dye is inhibited through the large steric hindrance effect of the bridge ring structure; the dye shows bright fluorescence emission in non-polar and polar solvents, also has a very strong fluorescence quantum yield in a solid state, and shows a quantum yield greater than 94% when the dye is dispersed in an EVA (Ethylene-Vinyl Acetate) film.

In one embodiment, a method is disclosed to determine one or more biological cells of interest to identify and count in a mixed biological sample fluid with differing biological cells. The method can include selecting cell markers associated for biological cells to which conjugated antibodies can attach with differing fluorescent dyes; displaying a panel builder graphical user interface window to display a co-expression matrix by biological cell type to assist in selecting cell markers to assign co-expression; and selecting cell markers to assign co-expression with an input device.

Compositions, testing chambers and methods for testing a fuel sample for microbial contamination (including fuels treated with a biocide) are provided, which comprise: a quantity of hydrocarbon fuel; a microbial contamination wherein the microbial contamination further comprises nucleic acid in the form of both DNA, RNA or a combination thereof, and an analyzing solution; wherein the analyzing solution comprises at least six (6) primer pairs for amplification of at least one target locus, wherein at least one primer of each pair of primers is labeled with a fluorescent dye and wherein at least one of the primer pair binds to the nucleic acid of the microbial contamination.

The invention discloses a liquid-phase protein chip method for simultaneously detecting three kinds of porcine coronaviruses and a kit thereof, which comprises the following steps of: after activating and coupling microspheres, respectively coating antigen on porcine delta coronavirus N protein, porcine acute diarrhea syndrome coronavirus N protein and porcine epidemic diarrhea virus N protein by using the microspheres; matching the two fluorescent dyes according to different concentrations to form fluorescent coding microsphere combination arrays with different energy levels; the microspheres are analyzed one by one based on the flow principle, red laser recognizes microsphere codes, green laser reads fluorescence intensity of report molecules, computer analysis and processing are carried out, and fluorescence signals are converted into quantitative detection results; and determining the optimization of the detection conditions of the liquid-phase protein chip after the liquid-phase protein chip test. The antigens coated on the microspheres only specifically react with corresponding antibodies and are not combined with antibodies generated by other pathogens; the PDCoV, SADS-CoV and PEDV antibodies in the sample to be detected can be rapidly, effectively and accurately detected, and reference is provided for prevention of the porcine coronavirus.

The invention relates to an endoscope imaging system and a light source thereof, the light source comprises a laser light source, a visible light source and a light combiner, the laser light source is used for outputting laser of at least two different wavebands, the laser of the different wavebands is used for exciting different fluorescent dyes, and the visible light source is used for outputting visible light; the light combiner is used for combining the laser and the visible light into a combined light beam and outputting the combined light beam to the endoscope; wherein the laser light source is a single light-emitting device, the single light-emitting device comprises a single substrate and at least two laser chips arranged on the single substrate, the at least two laser chips are used for emitting laser of different wave bands, and the single light-emitting device further comprises a single light outlet and a single optical fiber; and laser emitted by the at least two laser chips is output to the single optical fiber through the single light outlet. The light source can independently or simultaneously output laser of different wave bands to excite different fluorescent dyes, and the layout of an endoscope imaging system is simplified by adopting the light source.

A method of medical multi-dye fluorescence imaging. The method including: capturing fluorescent images of an operating field in which two or more different fluorescent dyes are present in successive sequences of images with a surgical or diagnostic imaging device, each sequence of images alternating through different modes of excitation lighting adapted for at least one each of two or more of the two or more different dyes, and processing the captured images of each successive sequence of images in combination with each other. The processing comprising determining the respective local distributions of the two or more different fluorescence dyes causing the observed different distributions of brightness in the two or more different fluorescent images of the sequence and producing one or more images displaying the determined local distributions of the two or more different fluorescent dyes separately.

The invention discloses a near-infrared organic fluorescent dye with mechanical responsiveness, and relates to the technical field of organic fluorescent dyes, the near-infrared organic fluorescent dye with mechanical responsiveness is prepared through seven-step reaction, an intermediate M1 containing a hydroxyl active end is firstly prepared, then intermediates M2, M3 and M4 are obtained through multi-step reaction, a target dye is obtained after purification, and finally surface functionalized nano-particles are self-assembled, so that the near-infrared organic fluorescent dye with mechanical responsiveness is obtained. The preparation method has the advantages that by designing a conjugate regulation and control structure of a rigid chromophore core and a flexible bridging unit, a mechanical response group is directionally grafted to the tail end of the flexible bridging unit through a CuAAC click chemical reaction; the problems of low fluorescence quantum yield and high response threshold caused by conjugation conflict of a traditional mechanical response group and a near-infrared chromophore are solved, the mechanical response threshold is reduced to a low stress range required by biomechanical detection, collaborative optimization of mechanical responsiveness and near-infrared fluorescence performance is realized, and the method is suitable for the fields of high-sensitivity biological stress monitoring and the like.

The invention discloses a fluorescent dye for shoe soles as well as a preparation method and application of the fluorescent dye, and belongs to the technical field of fluorescent materials for shoes. The fluorescent dye has a structure as shown in a formula 1. The preparation method comprises the following steps: synthesizing an intermediate through Buchwald-Hartwisting arylation reaction, and carrying out Suzuki coupling to obtain a target product. The dye inhibits aggregation fluorescence quenching through a rigid plane structure and a deuterated group, the HOMO-LUMO energy gap is regulated to 2.8-3.2 eV, the dye has the broad band emission of 450-600 nm, and the fluorescence quantum efficiency reaches 0.55-0.75. When the brominated butyl rubber is applied to a sole formula, the brominated butyl rubber comprises the following components in parts by weight: 40-60 parts of brominated butyl rubber, 0.1-2 parts of fluorescent dye and the like, and a finished product is prepared by mixing, vulcanizing and foaming. Compared with a traditional dye, the fluorescence intensity is improved, the photooxidation resistance is enhanced, the dissolution rate is reduced by 60% or above, and the dye has excellent humidity and heat resistance and material compatibility.

In one embodiment, a method of building an optimized color flow cytometry panel is disclosed using a full spectrum flow cytometer with five excitation lasers and five corresponding detection modules. In another embodiment, a graphical user interface is disclosed generated by a server computer from a fluorochrome database and displayed by a client computer to assist in the selection of a set of fluorochromes for use in an assay to analyze biological samples. The GUI can display spectra graphs to visually show how fluorochromes may overlap and can generate similarity indexes for the paired fluorochrome interference and a complexity index for overall many to many interferences generated by a selected group or set of fluorochromes.

Compositions, testing chambers and methods for testing a fuel sample for microbial contamination (including fuels treated with a biocide) are provided, which comprise: a quantity of hydrocarbon fuel; a microbial contamination wherein the microbial contamination further comprises nucleic acid in the form of both DNA, RNA or a combination thereof, and an analyzing solution; wherein the analyzing solution comprises at least six (6) primer pairs for amplification of at least one target locus, wherein at least one primer of each pair of primers is labeled with a fluorescent dye and wherein at least one of the primer pair binds to the nucleic acid of the microbial contamination.

The present application relates to the technical field of molecular biology detection, in particular to an absolute antibody Taq DNA polymerase activity determination method based on real-time fluorescence quantitative detection, comprising the following steps: firstly, establishing a standard curve of DNA content and fluorescence value by reacting different concentrations of double-stranded lambda DNA with fluorescent dyes; then, performing an extension reaction in a specific reaction system using M13 single-stranded DNA as a template, recording fluorescence values in real time, calculating net fluorescence values and establishing a linear relationship between the net fluorescence values and reaction time; converting the amount of newly generated double-stranded DNA at different time points by using the standard curve, further calculating the consumption amount of dNTPs, and finally obtaining the absolute activity of the antibody Taq DNA polymerase according to the definition of the activity of the antibody Taq DNA polymerase. The whole process combines fluorescence detection and a standard curve to realize accurate determination of enzyme activity.

The invention relates to the technical field of sensing of magnetic nano photoelectric materials, in particular to a responsive microsphere based on a three-dimensional magnetic photonic crystal and a preparation method and application thereof, and the responsive microsphere is formed by arranging polyhydroxy compound modified magnetic nanoparticles in a spherical polymer into a non-close packing face-centered cubic or body-centered cubic structure. The responsive microsphere based on the three-dimensional magnetic photonic crystal is easy in color distinguishing, has no angle dependence, does not need to depend on a fluorescent dye or a radioactive label, can realize real-time and in-situ monitoring through own optical characteristics, breaks through the bottlenecks of sensitivity and resolution of a traditional sensor, realizes visual detection, simplifies the detection process, and is suitable for mass production. The cost is reduced. According to the responsive microsphere based on the three-dimensional magnetic photonic crystal, the substance diffusion path is shortened through the small size of the responsive microsphere, and the response speed is remarkably increased in combination with the high specific surface area.

The invention belongs to the field of biosensing and microbiological detection, and relates to a staphylococcus aureus rapid counting method based on fluorescence confinement discretization. According to the method, oat bran is used as a natural carbon source, lysozyme active fragments are used as targeting ligands, targeting carbon quantum dots are synthesized through a one-step hydrothermal method, the targeting carbon quantum dots are modified by fluorescent dye SRB to obtain luminescence enhanced carbon quantum dots, and the luminescence enhanced carbon quantum dots can be specifically combined with cell walls of staphylococcus aureus, so that the staphylococcus aureus has stable fluorescence signals. And then, by taking the marked thalli as a core layer and a high-molecular polymer as a shell layer, preparing a core-shell nanofiber membrane by adopting a coaxial electrostatic spinning technology to realize spatial confinement and monomer discretization of the thalli, so that a group fluorescence signal is converted into a single-thalli distinguishable signal, and rapid and sensitive quantitative detection of the staphylococcus aureus is realized. The method is simple, convenient, high in sensitivity, suitable for rapid visualization and quantitative detection of staphylococcus aureus in foods such as dairy products, meat products and beverages, and wide in application prospect.

This invention provides a medical marker that offers excellent mounting stability, improved retention period, and superior visibility from the outside of tubular organs. [Solution] The medical marker 100 according to the present invention is a medical marker 100 that is implanted in a tubular organ in the body, and comprises a main body portion 120 extending in the longitudinal direction L, and two or more locking portions 150 that extend from a proximal end portion 151 fixed to a part of the distal side of the main body portion 120 to a tip portion 154. The locking portions 150 are configured to be deformable into a curved state in which they are curved laterally from the proximal end portion 151 so that the tip portion 154 is positioned proximal to the proximal end portion 151, and an extended state in which they extend in the longitudinal direction L from the proximal end portion 151. The main body portion 120 or the locking portions 150 are arranged with a phosphor containing a fluorescent dye that emits fluorescence in a predetermined wavelength range when irradiated with excitation light.

The organic electroluminescent device includes a first electrode, a second electrode and an organic layer located between the first electrode and the second electrode, the organic layer includes a light-emitting layer, the light-emitting layer includes a host material, a thermally activated delayed fluorescence sensitizer and a green fluorescent dye, the green fluorescent dye includes a structure as shown in formula I. A thermally activated sensitized fluorescence technique is used, and the green fluorescent dye of a specific structure in combination with the sensitizer and the host material is used, so as to achieve the effects of narrowing the spectrum of a device and improving the green color purity. The efficiency of the organic electroluminescent device is equivalent to that of a phosphorescent green light device, so that a display panel including the organic electroluminescent device has a large display color gamut area.

The present disclosure provides improved and useful techniques for cross-standardization of flow cytometry instruments and, particularly, spectral flow cytometry instruments. Aspects of the disclosure include methods of calibrating a flow cytometer having a plurality of fluorescence channels. Methods of interest utilize calibration sets of bead populations, wherein each bead population of the calibration set includes a different fluorophore attached to a surface thereof and the calibration set includes a number of bead populations that is less than the number of fluorescence channels of the flow cytometer. Flow cytometers, non-transitory computer-readable storage media, and kits including, e.g., calibration sets of bead populations for carrying out the subject methods are also provided.

The invention discloses a method for applying photoswitch fluorescent polymers to dynamic fluorescent falsification prevention, which comprises the following steps: taking methyl acrylate, 1, 6-hexanediol diacrylate, a photoinitiator 819, a green fluorescent dye BDPMA and one of three photochromic spiropyrans SPMA, SPAMA and SPBMA as raw materials, and preparing three photoswitch fluorescent polymers P1, P2 and P3 in a photopolymerization manner. The P1, P2 and P3 are irradiated by ultraviolet light and placed in room-temperature darkness, red fluorescence generated by the P1, P2 and P3 can be gradually and spontaneously converted into green fluorescence, due to the structural difference of the three spiropyrans, the fluorescence discoloration rates of the three polymers show obvious difference, and a new dynamic fluorescent anti-counterfeit label is constructed by utilizing the difference. The photoswitch fluorescent polymer obtained by the invention has the advantages of simple preparation method, stable structure and good machinability, is suitable for amplification synthesis and practical application, and has wide application prospects in the fields of optical anti-counterfeiting and information encryption.

The invention discloses a method for detecting perfluorinated compounds by using a sensor array composed of dyes, comprising the following steps: S1, preparing a fluorescent dye solution: preparing a fluorescein diluent, a coumarin 7 diluent, and a cyanine diluent; S2, preparing a pollutant solution: preparing diluted PFBA solution, PFOA solution, PFDA solution, PFUdA solution, and PFDoA solution; S3, constructing a fluorescent sensor array and detecting perfluorinated compounds: placing a sample in a fluorescence spectrometer for detection. The dyes used in the detection method of the invention are easy to obtain and have strong fluorescence stability. The sensor array can detect perfluorinated compounds with similar structures, and the operation is simple.

The invention discloses an application of a copper nano-cluster with stable ascorbic acid in detection of p-nitrophenol, the copper nano-cluster with stable ascorbic acid is used as a fluorescent probe, and the detection of p-nitrophenol is realized through a fluorescent sensing method. Due to the quenching effect of p-NP, the fluorescence intensity of the system is obviously reduced in the presence of p-NP. The p-NP concentration can be quantitatively detected by comparing the fluorescence intensity of the copper nano-cluster and the fluorescence intensity of a quenching system. The method is simple, sensitive and high in selectivity, does not need complex fluorescent dye labeling, shows remarkable potential for p-NP detection, and has a good application prospect in the aspects of food, medicine and the like.

The invention discloses an application of a cyclochalcone cationic compound as a fluorescent dye in the field of pressing discoloration and detection of trace water or protonic solvents in solvents, the structural formula of the cyclochalcone cationic compound is shown in formula I. The monitoring of the trace water or protonic solvents in the solvents can be realized in the'opening 'process of a fluorescent signal of the dye; the dye fluorescence signal'closing 'phenomenon can be observed through grinding, and the process can be applied to the fields of stress detection, ink-free writing, anti-counterfeiting and the like. The dye can be prepared into test paper, developing powder and other test carriers for portable application, has the advantages of simplicity in operation, low cost, real-time detection and the like, and has the potential of large-scale popularization and application.

The present application relates to the technical field of fluorescent dyes, in particular to an asymmetric pentamethine cyanine compound. The compound comprises a structure as shown in formula I. The compound provided by the present application has good water solubility, near-infrared absorption, high fluorescence quantum yield and good molar absorption coefficient, and can be used in nucleic acid labeling, DNA sequencing, cell imaging, protein labeling or specific recognition of amino acids.

Disclosed herein are viewing systems configured for viewing an image of a biological object illuminated with light and a viewing device (e.g., loupes, glasses, etc.) and methods for operation thereof. The viewing device comprises a plurality of viewing modes: a white light mode, a fluorescent light mode, and an overlay mode, and a shutter that opens and closes in accordance with the viewing mode. An overlay image is an image of the biological object when the biological object is illuminated with both white light and fluorescent light, causing the overlay image to be an overlay of the white light image and the fluorescent light image. The disclosed systems and methods allow a user (e.g., a surgeon, medical staff, etc.) to preserve the ergonomics offered by eyewear while providing fluorescent viewing capabilities, particularly with visible fluorescent dyes.

Providing control over the nucleic acid amplification process. [Solution] Methods, systems, reaction mixtures, kits, apparatus, and systems for monitoring nucleic acid amplification reaction mixtures for abnormalities. Deviating temperatures and buffer compositions from normal or expected conditions may occur during the preparation of the reaction mixture, and / or may be caused by errors in the functionality of the nucleic acid amplification system. An exemplary method includes determining fluorescence-based values ​​from fluorophore-containing nucleic acid amplification reaction mixtures and determining whether those values ​​meet predetermined values ​​or indicate abnormalities.

The application provides a visual micro-nano optical waveguide refractive index sensing system, and a hardware part comprises a nano fiber taper coupled fluorescent doped polymer microwire; the nano fiber taper coupled fluorescent doped polymer microwire is coupled by a nano fiber taper and a fluorescent doped polymer microwaveguide; the nano fiber taper is obtained by adopting a flame tapering method from a standard single-mode optical fiber; the fluorescent doped polymer microwaveguide is directly prepared by wire drawing from a mixed solution doped with a fluorescent dye; the nano fiber taper and the fluorescent doped polymer microwaveguide placed in a channel of an optical substrate jointly form an evanescent wave coupling structure; a signal analysis part enhances, edge extracts, smoothes and peak extracts a fluorescent image of the microwire through an image analysis algorithm, obtains an oscillation period of a fluorescent light path in the microwire under a current environment, establishes a response relationship between the light path period and the environmental refractive index, realizes direct real-time obtaining of environmental change conditions from optical images of a sensing unit shot by a camera, and thus obtains the change of the refractive index.