853 results about "Fluorochrome Dye" patented technology

Protected fluorescent reagent compounds and their methods of synthesis are provided. The compounds are useful in various fluorescence-based analytical methods, including the analysis of highly multiplexed optical reactions in large numbers at high densities, such as single molecule real time nucleic acid sequencing reactions. The compounds contain fluorescent dye elements, that allow the compounds to be detected with high sensitivity at desirable wavelengths, binding elements, that allow the compounds to be recognized specifically by target biomolecules, and protective shield elements, that decrease undesirable contacts between the fluorescent dye elements and the bound target biomolecules and that therefore decrease photodamage of the bound target biomolecules by the fluorescent dye elements.

A fluorescence imaging device detects fluorescence in parts of the visible and invisible spectrum, and projects the fluorescence image directly on the human body, as well as on a monitor, with improved sensitivity, video frame rate and depth of focus, and enhanced capabilities of detecting distribution and properties of multiple fluorophores. Direct projection of three-dimensional visible representations of florescence on three-dimensional body areas advantageously permits view of it during surgical procedures, including during cancer removal, reconstructive surgery and wound care, etc. A NIR laser and a human visible laser (HVL) are aligned coaxially and scanned over the operating field of view. When the NIR laser passes over the area where the florescent dye is present, it energizes the dye which emits at a shifted NIR frequency detected by a photo diode. The HVL is turned on when emission is detected, providing visual indication of those positions.

The present invention provides one kind of reagent for simultaneously detecting several kinds of small molecular compounds and its preparation process and usage. The preparation process includes the following steps: preparing coded microsphere reagent, and preparing one of the small molecular compound detecting antibody probes, including fluorescent quantum dot antibody probes, fluorescent quantum dot secondary antibody probes, fluorescent dye antibody probes and fluorescent dye secondary antibody probes. The method of detecting small molecular compounds with the antibody probe may be a direct antibody probe method or a indirect antibody probe method. The present invention may be used in detecting residual veterinary medicine, pesticide, banned drug, misused medicine and other small molecular compounds in food, agricultural product, living animal body and human body, and has the advantages of high sensitivity, high repeatability, etc.

The invention discloses a nucleic acid analyzing method based on cascade HCR (hybridization chain reaction), and belongs to the field of molecule detection. The nucleic acid analyzing method is characterized in that on the basis of single HCR, two stages of HCR reaction are designed, and the signals can be further amplified on the basis of single HCR; the hairpin nucleic acid probes marked with multiple different fluorescent dyes can be alternatively hybridized by a final target product, so as to form a branched DNA (deoxyribonucleic acid) nanometer structure; the signal output is provided bythe fluorescent resonance energy transfer, and the concentration of the target nucleic acid can be judged according to the change value of the fluorescence intensity of fluorophore. The nucleic acid analyzing method has the advantages that the nucleic acid analyzing method can be used for in-vitro detection of DNA and miRNA (miniature ribonucleic acid), the specificity is good, and the sensitivityis high; the nucleic acid analyzing method can be used for the imaging analysis of miRNA in cells, and be favorable for the early diagnosis on cancers.

The invention relates to a fluorescence probe for detecting intracellular hydrogen sulfide and a preparation method and application of the fluorescence probe. Cy7 fluorochrome and sodium acetate are dissolved in anhydrous dimethylformamide (DMF), heating reflux is carried out for 2-4 hours under protection of Ar gas, cooling and suction filtration are carried out on an obtained mixture, mother liquor is crystallized under the low temperature of minus 4-20 DEG C, and keto form cyanine reddish brown crystals are obtained after the suction filtration; toluoyl benzoic acid and oxalyl chloride are dissolved in anhydrous CH2Cl2, then a bit of DMF is added, mixing is carried out for 2-6 hours under the temperature of 0 DEG C, and toluoyl benzoyl chloride is obtained; and keto form cyanine and triethylamine are dissolved in the anhydrous CH2Cl2 and are cooled to be 0 DEG C, the toluoyl benzoyl chloride is added dropwise, mixing is carried out for 10-30 minutes under the temperature of 0 DEG C, the mixture is heated to be in the room temperature, mixing is carried out overnight, suction filtration and spinning drying are carried out, separation is carried out through a chromatographic column, green solids are obtained after the spinning drying, and the structural formula is as follows. The fluorescence probe is simple in composition, high in detection speed, free of long-term incubation in a detection process, high in sensitivity, and capable of detecting intracellular endogenic hydrogen sulfide.

A system and method for confocal imaging of tissue in vivo and in situ, e.g., for minimally invasive diagnosis of patients. A catheter is provided that has a dye carrier coupled to the distal end of a fiber optics bundle, which allows for the introduction of at least one fluorescent dye therein the dye carrier into a portion of the tissue of interest of a subject or patient when the dye carrier is selectively brought into contact with the portion of the tissue of interest. The resulting confocal images permit the acquisition of diagnostic information on the progression of diseases at cellular/tissue level in patients. Furthermore, a system for ECG-triggered image acquisition is provided.

A fluorescence imaging device detects fluorescence in parts of the visible and invisible spectrum, and projects the fluorescence image directly on the human body, as well as on a monitor, with improved sensitivity, video frame rate and depth of focus, and enhanced capabilities of detecting distribution and properties of multiple fluorophores. Direct projection of three-dimensional visible representations of florescence on three-dimensional body areas advantageously permits view of it during surgical procedures, including during cancer removal, reconstructive surgery and wound care, etc. A NIR laser and a human visible laser (HVL) are aligned coaxially and scanned over the operating field of view. When the NIR laser passes over the area where the florescent dye is present, it energizes the dye which emits at a shifted NIR frequency detected by a photo diode. The HVL is turned on when emission is detected, providing visual indication of those positions.

Provided are compounds, methods and kits for identifying in cells of interest organelles including nuclei and a wide variety of organelles other than nuclei (non-nuclear organelles), as well as cell regions or cell domains. These compounds and methods can be used with other conventional detection reagents for identifying the location or position or quantity of organelles and even for distinguishing between organelles in cells of interest.

Disclosed herein are compositions and methods for imaging nerve cells. The composition comprises a fluorescent dye; and a viral component selected from a neurotropic, replication-defective virus, a viral protein of a neurotropic virus, and a capsid of a neurotropic virus. Although the fluorescent dye in itself cannot penetrate nerve cells, the fluorescent dye is bound to the viral component to form a dye/viral component complex that is capable of penetrating nerve cells.

The invention provides type I unsymmetrical cyanine fluorescent dye, wherein X, n, R1, R2, R3, R4, R5 and Y<-> are defined as in the instruction book. The invention also provides the conjugate and the preparation method of the fluorescent dye, the combination containing the fluorescent dye and the biological dyeing method by using the fluorescent dye or the combination.

The present invention relates to novel fluorinated 3,6-diaminoxanthene compounds derived from the basic structural formula (I) and to their uses as photostable fluorescent dyes, e.g. for immunostainings and spectroscopic and microscopic applications, in particular in conventional microscopy, stimulated emission depletion (STED) reversible saturable optically linear fluorescent transitions (RESOLFT) microscopy, and fluorescence correlation spectroscopy. The claimed compounds are also useful as molecular probes in various spectroscopic applications.

Systems and methods for analyzing blood samples, and more specifically for performing a white blood cell (WBC) differential analysis. The systems and methods screen WBCs by means of fluorescence staining and a fluorescence triggering strategy. As such, interference from unlysed red blood cells (RBCs) and fragments of lysed RBCs is substantially eliminated. The systems and methods also enable development of relatively milder WBC reagent(s), suitable for assays of samples containing fragile WBCs. In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye, which corresponds in emission spectrum to an excitation source of a hematology instrument; (b) using a fluorescence trigger to screen the blood sample for WB Cs; and (c) using measurements of (1) axial light loss, (2) intermediate angle scatter, (3) 90° polarized side scatter, (4) 90° depolarized side scatter, and (5) fluorescence emission to perform a differentiation analysis.

The invention provides the appraisal and selective method for the antineoplastic drug based on the cell micrograph information, which uses a sort of selection and appraisal hardware system to appraisal and select the antineoplastic drug by different fluorescence dye marking and measuring the multicellular parameter change in cell. The hardware system is made up of the high precision electric hydrous platform, the fluorescence vision system, the image collecting and processing system and working station. The diacetoxyl fluoresceine dyeing measures the active cell number; the double dyeing method of Hoechst33342 and iodized pyridine appraise the drug inducing the cell die; the three dyeing method of FDA, Hoechst33342 and PI analyzes the die mode induced by drug. The invention can measure at least two kinds of single cell or cell subgroup which expresses different drone cell organ. The method is in reason and can be used in study of drug action mechanism and selecting the high hedonic drug and toxicity analyzing, which can be used in selecting drug and appraising the drug toxicity.

The invention relates to an organic electroluminescent device, a display panel and a display device. The organic electroluminescent device comprises a first electrode, a second electrode and an organic layer located between the first electrode and the second electrode. The organic layer comprises a light-emitting layer, the light-emitting layer contains a main material, a thermal activation delayed fluorescence sensitizer and a green fluorescent dye, and the green fluorescent dye has a structure as shown in a formula I. The thermal activation sensitization fluorescence technology is used, andthe green fluorescent dye with a specific structure, the sensitizing agent and the main material are matched for use so that the effects of narrowing the spectrum of the device and improving the colorpurity of green light are achieved, the device has the efficiency equivalent to that of a phosphorescent green light device, and the display panel comprising the device has a relatively high displaycolor gamut area.

Methods for differentially identifying cells in an instrument employ compositions containing a combination of selected antibodies and fluorescent dyes having different cellular distribution patterns and specificities, as well as antibodies and fluorescent dyes characterized by overlapping emission spectra which form non-compensatable spectral patterns. When utilizing the compositions described herein consisting of fluorescent dyes and fluorochrome labeled antibodies with overlapping spectra that cannot be separated or distinguished based upon optical or electronic compensation means, a new fluorescent footprint is established. This new fluorescent footprint is a result of the overlapping spectra and the combined cellular staining patterns of the dyes and fluorochrome labeled antibodies chosen for the composition. The new fluorescent footprint results in histogram patterns that are useful for the identification of additional cell populations or subtypes in hematological disease.

The invention provides a red fluorescence gold nanocluster as well as a preparation method and an application thereof and belongs to preparation and applications of fluorescence nano materials. According to the preparation method, cheap egg white is used for rapidly reducing and coating HAuCl4, and gold nanocluster AuNCs@EW giving red fluorescence is synthesized. The preparation method is simple, rapid and low in cost. The prepared nanocluster can be used as fluorescent dye, can be used for detecting mercury ions and further can be used for detecting GSH (glutathione).