697 results about "Fluorochrome Dye" patented technology

To provide a method for detecting the presence of multiple specific nucleic acid sequences or single nucleotide polymorphisms in a temperature-dependent fashion.SOLUTION: Provided is a method, in which an invasive oligonucleotide and a primary probe oligo (a 5' flap oligonucleotide) hybridize to a target strand nucleic acid to form an invasive cleavage structure cleavable by a FEN-1 endonuclease to release a cleaved 5' flap (primary cleaved flap); in a secondary reaction, the primary cleaved flap from the primary reaction hybridizes to a FRET cassette 1, a hairpin oligonucleotide labeled with a fluorescent dye and a quencher molecule, to form a second invasive cleavage structure that is cleavable by a FEN-1 endonuclease at a site between the fluorophore and a quencher; and cleavage of FRET cassette 1 separates the quencher from the fluorophore, such that fluorescence signal from the fluorophore can be detected.SELECTED DRAWING: Figure 1

There is proposed a method for detecting a presence of a fluorescence pattern type on an organ segment via immunofluorescence microscopy and digital image processing. The steps comprise: provision of the organ segment, incubation of the organ segment with a liquid patient sample, incubation of the organ segment with secondary antibodies which have been labelled with a fluorescent dye, acquisition of a fluorescence image of the organ segment in a colour channel corresponding to the fluorescent dye, and provision of the fluorescence image to a neural network. What takes place by means of the neural network is simultaneous determination of segmentation information through segmentation of the fluorescence image and, furthermore, of a measure of confidence indicating an actual presence of the fluorescence pattern type. What further takes place is determination, on the basis of the previously determined segmentation information, of at least one sub-area of the fluorescence image that is relevant to formation of the fluorescence pattern type, determination, on the basis of the previously determined at least one sub-area, of validity information indicating a degree of a validity of the measure of confidence, and output of the measure of confidence depending on the validity information.

The invention provides a monitoring device for simultaneously monitoring multiple pollutants in a water environment based on an echo wall mode liquid crystal microcavity and a control method thereof, and belongs to the technical field of optical microsensing. Cholesteric liquid crystal solution doped with fluorescent dye DCM is injected into liquid to be detected through a conical capillary microtube to form a plurality of liquid microsphere cavities; the fluorescent dye DCM in the liquid crystal microspheres emits fluorescence under the excitation of 532 nm laser pulse, high-quality echo wall mode laser emission is generated under the limiting action of the microcavity, and a spectrograph is used for recording a laser spectrum; when the concentration of pollutants in a water environment slightly changes, the change of the refractive index of the liquid crystal causes the change of the wavelength of the laser, so that the spectrum drifts, and high-sensitivity sensing is realized; the invention designs a real-time, quantitative and visual water environment pollutant detection sensor which has a wide application prospect in the field of water environment protection.

The invention relates to the technical field of composite materials, and provides a cellulose nanocrystalline composite material as well as a preparation method and application thereof. The cellulose nanocrystal composite material provided by the invention comprises cellulose nanocrystals and a fluorescent dye, and the fluorescent dye comprises a Hurst fluorescent dye. According to the present invention, the hydrophilic Hurst fluorescent dye is selected to be compounded with the cellulose nanocrystal, such that the composite material with characteristics of circular polarization fluorescence property, phosphorescence emission property and high fluorescence quantum yield is provided, and the material is environmentally friendly, has small influence on the environment during preparation and use, and has great application potential in the anti-counterfeiting field.

Compounds useful as fluorescent or colored dyes are disclosed. The compounds have the following structure (I):or a stereoisomer, tautomer or salt thereof, wherein R1, R2, R3, R4, R5, L1, L2, L3, L4, M1, M2, m and n are as defined herein. Methods associated with preparation and use of such compounds is also provided.

Compounds useful as fluorescent or colored dyes are disclosed. The compounds have the following structure (I):or a stereoisomer, tautomer or salt thereof, wherein R1, R2, R3, R4, R5, L1, L2, L3, L4, M, m and n are as defined herein. Methods associated with preparation and use of such compounds are also provided.

The invention discloses an alanine aminotransferase detection device and method based on a liquid crystal microcavity array, and belongs to the field of optical sensing. The device comprises a functional liquid crystal microcavity array, an excitation optical fiber and a collection optical fiber, the microcavity array is composed of liquid crystal microcavities doped with fluorescent dye and stearic acid, and the multiple microcavities are independently distributed and serve as optical sensing units; the excitation optical fiber is used for synchronously exciting the microcavity to generate an echo wall mode, and the acquisition optical fiber acquires resonance spectrum signals of each channel in real time; the functionalized liquid crystal microcavity is converted into a bipolar configuration from a radial configuration in an enzymatic reaction, echo wall mode spectrum red shift is caused, spectrum shift time is in negative correlation with enzyme concentration, and dual-channel detection is realized by matching with conversion time of a polarized light pattern. According to the detection structure based on the liquid crystal microcavity array provided by the invention, high-flux, real-time and label-free rapid detection on the concentration of alanine aminotransferase can be realized.

The invention discloses a core-shell structure Au-coated CeO2 nano-catalyst and a preparation method and application thereof, and belongs to the technical field of core-shell structure catalysts.The preparation method of the catalyst comprises the following steps that S1, a chloroauric acid solution and a low-concentration cerium chloride solution are added into deionized water and stirred and mixed at the temperature of 5 DEG C, then an ammonia water solution is rapidly added, stirring and mixing are conducted at the temperature of 5 DEG C, and a mixed solution is obtained; quickly adding a high-concentration cerium chloride solution, and continuously stirring for 30 minutes to obtain a mixed solution; s2, carrying out centrifugal separation on the mixed solution to retain a precipitate, washing the precipitate with deionized water and absolute ethyl alcohol, and carrying out freeze drying to obtain a catalyst precursor; s3, performing cleaning treatment and annealing treatment on the catalyst precursor, and cooling to room temperature to obtain a core-shell structure Au-coated CeO2 nano-catalyst; when the catalyst is applied to catalytic synthesis of the BODIPY fluorescent dye, excellent catalytic performance and recycling performance are shown, and the yield of the BODIPY fluorescent dye can be remarkably increased.

Disclosed in the present invention is a stimulated emission depletion and super-resolution fluorescence lifetime imaging method, which is applied to the technical field of optical microscope imaging. The method provided in the present invention combines the stimulated emission depletion and fluorescence lifetime imaging principles, a depletion laser pulse frequency used therein is half of an excitation light pulse frequency, and a time interval between two pulse sequences is adjusted, such that a depletion laser pulse follows an adjacent excitation light pulse; and analysis processing is performed on collected fluorescence lifetime data, such that a super-resolution fluorescence lifetime image is obtained, thereby solving the problem of the fluorescence lifetime of a stimulated emission depletion and fluorescence lifetime imaging microscope being significantly shortened under the action of a high-energy depletion laser, and thus accurately reflecting real information of a fluorochrome and a micro-environment. Therefore, the innovative technique provides important technical support for life science research, significantly improves the accuracy and effectiveness of research, and promotes further development in the field of biomedicine.

The invention provides a fluorescent biosensor for detecting N-terminal B-type pro-natriuretic peptide as well as a preparation method and application of the fluorescent biosensor. The fluorescent biosensor is mainly composed of a palladium nanosheet and a fluorescent dye modified nucleic acid aptamer of N-terminal B-type pro-natriuretic peptide. Fluorescence resonance energy transfer and an inner filtering effect can be generated between the palladium nanosheet and the fluorescent dye modified nucleic acid aptamer of the N-terminal B-type pro-natriuretic peptide, so that fluorescence of a system is quenched, a fluorescence signal is closed, and the fluorescent dye modified nucleic acid aptamer can be used for specifically recognizing the N-terminal B-type pro-natriuretic peptide, so that the sensitivity of the system is greatly improved. The fluorescent dye modified nucleic acid aptamer is far away from the palladium nanosheet, the system fluorescence signal is open, the fluorescence recovery efficiency and the logarithm of the N-terminal B-type pronatriuretic peptide have a good linear relationship in the concentration range of 0.8-30 pg / mL, and the detection limit is 0.21 pg / mL. The sensitive, rapid and specific detection of the N-terminal B-type pro-natriuretic peptide in a short time is realized through the change of the fluorescence intensity of the system.

Provided herein are single domain antibody / polymer fluorescent tandem dye conjugates. Aspects of the conjugates provided herein include single domain antibodies having one or more polymer tandem fluorescent dyes conjugated thereto. In some cases, a polymer tandem fluorescent dye includes: (i) a polymer backbone consisting of non-conjugated repeating units; (ii) a plurality of pendant donor chromophore groups, each group independently attached to a non-conjugated repeating unit of the polymer backbone; and (iii) one or more pendant acceptor fluorophores attached to non-conjugated repeating units of the polymer backbone wherein the pendant donor and acceptor fluorophores are in an energy transfer relationship. Also provided herein are methods of using the conjugates, for example, methods for assessing the presence of a target analyte in a sample, and kits comprising conjugates useful in embodiments of the methods.

The invention provides a method for measuring and calculating the article position of continuous silver ore with the particle size of 0.037 mm or above, and belongs to the technical field of mineral grade detection. The silver grade in the sample is measured and calculated through screening; leaching and measuring the content of silver monomers and continuous silver minerals, and reselecting to obtain a heavy sand product; carrying out fluorescent dyeing on the heavy sand product, and selecting monomer silver minerals; analyzing the color of the silver mineral through point scanning data of a scanning electron microscope, melting the monomer silver mineral to obtain the volume of the monomer silver mineral, and calculating the content of the monomer silver mineral; and carrying out conversion to obtain the continuous silver ore article position with the size of more than 0.037 mm. According to the method, the target mineral is rapidly and accurately obtained by adopting density medium reselection and fluorescent dye tracing; introducing a scanning electron microscope to measure and calculate the actual density of the silver mineral to replace the theoretical density, smelting the silver mineral in a mold, and carrying out volume measurement and calculation to obtain the actual metal amount closer to the actual silver mineral; measuring and calculating errors caused by impurities and irregular shapes of mineral particles are reduced; and the calculated grade of the silver mineral is more accurate.

The invention discloses an optical stealth color radiation refrigeration coating as well as a preparation method and application thereof, and relates to the technical field of optical stealth materials. The coating is prepared from the following raw materials in percentage by mass: 30 to 50 percent of nano-particles wrapped by fluorescent silicon dioxide shells, 30 to 35 percent of polydimethylsiloxane, 5 to 10 percent of auxiliaries and 30 to 40 percent of ethyl acetate, wherein the sum of the mass percent of all the components is 100 percent; according to the invention, the organic fluorescent dye is doped in the mesoporous silica and is coated with the silica shell, so that not only is the fluorescence aggregation quenching effect of the organic dye avoided and the maximum fluorescence intensity of the dye improved, but also the silica shell layer at the outer layer also plays a role of a protective layer; oxidation of ultraviolet rays, solvents and oxygen to the dye is shielded, and the light stability of the dye is improved.

The invention discloses preparation of a nitrogen-containing seven-membered fluorine-boron compound and application of the nitrogen-containing seven-membered fluorine-boron compound to a fluorescent anti-counterfeiting film, the structure of the compound is as follows: # imgabs0, the dye takes 2, 3, 3-trimethylindole and dibromo-2-pyrrolecarboxaldehyde as raw materials, under the action of acetic acid, piperidine and methylbenzene, a Knoevenagel condensation reaction is carried out, and the dibromo seven-membered fluorine-boron fluorescent dye is generated. The dibromo seven-membered fluorine boron fluorescent dye respectively reacts with phenylboronic acid, 4-cyanophenylboronic acid, p-methoxyphenylboronic acid and triphenylamine phenylboronic acid to obtain a final compound. The compound can act with acrylic resin to form a film on a thin film, and can be colored under natural light, and clear fluorescence can be seen under an ultraviolet lamp (365nm). The fluorescent dye is simple, easy to obtain, low in manufacturing cost, high in film forming speed with acrylic resin or a polyvinyl alcohol solution on a thin film, high in anti-counterfeiting coefficient, large in counterfeiting difficulty and huge in potential in the aspects of fluorescent anti-counterfeiting packaging films, OLDE and the like.

The invention belongs to the technical field of plant fungus molecular biology detection, and discloses an LAMP primer group, a kit and a detection method for detecting oat smut pathogenic bacteria. A group of LAMP (loop-mediated isothermal amplification) specific primers are designed according to a specific sequence on a whole genome of the oat smut pathogenic bacteria Ustilago hordei, results are judged through a real-time fluorescence quantification method, an agarose gel electrophoresis method and an SYBR Green I fluorescent dye developing method, and the oat smut pathogenic bacteria carried by oat seeds are detected. The LAMP detection method for the oat smut pathogenic bacteria, established by the invention, is strong in specificity, high in sensitivity, high in speed and low in cost, provides a new technical means for detection of the oat smut pathogenic bacteria carried by the oat seeds, and has relatively high practical application value.

An optical sensor patch for measuring water activity is disclosed. The optical sensor patch includes a sheet configured to be in equilibrium with water activity in an environment of the optical sensor patch. A pair of distinct fluorescent dyes are dispersed within the sheet. The fluorescence spectra of the optical sensor patch is dependent on the water activity in the environment of the optical sensor patch. An optical implant and systems and methods for using the optical sensor patch are also disclosed.

The invention provides an anticorrosive paint, a preparation method thereof, an anticorrosive coating and application. The provided anticorrosive paint comprises a polymer matrix containing reversible chemical bonds and microcapsules dispersed in the polymer matrix. The microcapsule comprises a core material and a capsule wall coating the core material, and the core material comprises a fluorescent dye; the reversible chemical bond in the polymer matrix is one or more of Diels-Alder bond, disulfide bond and hydrogen bond. The anti-corrosion coating prepared from the anti-corrosion paint can realize damage early warning and repair tracking.

The invention provides a benzobis (thiadiazole) near-infrared two-region fluorescent dye molecule and a preparation method thereof, and the preparation method comprises the following steps: by taking a benzobis (thiadiazole) derivative as a raw material, introducing 3, 4-ethylenedioxythiophene through a Stille cross-coupling reaction; the preparation method comprises the following steps: carrying out bromination with N-bromosuccinimide, introducing a cyanated dibenzocarbazole derivative through a Suzuki cross-coupling reaction, and finally carrying out reduction and cyclization reactions to synthesize the end cyano-functionalized near-infrared two-region fluorescent small molecular dye. The receptor-donor-receptor-donor-receptor (A '-D-A-D-A') structure near-infrared two-region fluorescence molecule disclosed by the invention has the properties of long absorption wavelength, high fluorescence quantum yield, high fluorescence brightness and the like, and has a wide application prospect in the field of near-infrared two-region fluorescence imaging.

The preparation method comprises the following steps: carrying out reflux reaction on 4-bromo-1, 8-naphthalic anhydride and primary amine in an organic solvent to obtain an intermediate product, and carrying out C-N coupling reaction on the intermediate product, a compound containing a nitrogen secondary amine electron-donating group, alkali and a catalyst in the organic solvent to obtain the double-state emission fluorescent dye. The two-state emission dye is obtained. An amino auxochrome group of a bridge ring structure is modified in a 4-bromine-1, 8-naphthalimide parent, the fluorescence quantum yield of the dye in a polar solvent is improved through the characteristic that nitrogen atom lone pair electrons of the nitrogen-containing bridge ring structure are not easy to overturn, and pi-pi stacking, which is not beneficial to emission, of the dye is inhibited through the large steric hindrance effect of the bridge ring structure; the dye shows bright fluorescence emission in non-polar and polar solvents, also has a very strong fluorescence quantum yield in a solid state, and shows a quantum yield greater than 94% when the dye is dispersed in an EVA (Ethylene-Vinyl Acetate) film.

An alpha channel is generated, typically in real time, using image data acquisition that contemporaneously captures image data representing the visible portion of the light spectrum and image data representing the invisible portion of the light spectrum. In some embodiments, the invisible portion of the light spectrum is generated by a fluorescent dye applied to an object or actor in a scene, while in other embodiments the invisible portion of the light spectrum is generated by a light source located behind the object or the actor.

The invention discloses an STR (short tandem repeat) fluorescence labeling composite amplification system, the STR fluorescence labeling composite amplification system comprises 6 STR loci on a human chromosome 15, 12 STR loci on a human chromosome 20 and corresponding 36 primer pairs, and each primer pair carries a fluorescent dye marker. The kit can be used as an effective supplement of an existing kit, the false positive rate can be remarkably reduced, the detection accuracy and reliability are effectively improved, an important basis is provided for prenatal diagnosis and genetic disease detection, and the kit has important clinical application value and wide market prospects.

In one embodiment, a method is disclosed to determine one or more biological cells of interest to identify and count in a mixed biological sample fluid with differing biological cells. The method can include selecting cell markers associated for biological cells to which conjugated antibodies can attach with differing fluorescent dyes; displaying a panel builder graphical user interface window to display a co-expression matrix by biological cell type to assist in selecting cell markers to assign co-expression; and selecting cell markers to assign co-expression with an input device.

The invention relates to the technical field of organic functional materials, in particular to a near-infrared second-region cyanine dye molecule as well as a preparation method and application thereof, the structural formula of the near-infrared second-region cyanine dye molecule is shown as a formula I or a formula II: # imgabs0 #, the cyanine dye molecule is modified by benzopyranium, the excitation wavelength and the fluorescence emission wavelength of the near-infrared second-region cyanine dye molecule both reach the near-infrared second region, and the near-infrared second-region cyanine dye molecule is a near-infrared second-region cyanine dye molecule. Strong absorption and emission are shown in a near-infrared second region, and the Stokes shift is greater than 50nm; the synthetic method of the fluorescent dye is simple, raw materials are easy to obtain, and the yield is high; the cyanine dye disclosed by the invention is excellent in photo-thermal stability and good in photo-thermal imaging effect, has a good phototherapy effect and has a great potential application prospect in the field of tumor treatment.

The invention discloses a light capture system based on a polybenzyl ether dendrimer as well as a preparation method and application of the light capture system. According to the light capture system, a core mono-cyano ethylene modified polybenzyl ether dendrimer is used as a light acquisition antenna and an energy donor, and a fluorescent dye is used as an energy receptor; the core mono-cyano ethylene modified polybenzyl ether dendrimer and the fluorescent dye are co-assembled in a solution to form a light capture system. The light capture system provided by the invention has excellent aggregation-induced emission performance, obviously enhanced fluorescence and narrower emission band, and can still maintain better energy transfer and ultrahigh antenna effect under a high donor-acceptor ratio; in addition, the preparation process is green and environment-friendly, and the light capture system is stable in structure and can be stored for a long time.

The invention discloses a single molecule positioning method based on high-speed phase-locked detection, which can perform high-precision single molecule positioning and imaging on a fluorescent sample under the condition of using a single laser light source. Molecular excitation of a sub-diffraction limit region is generated by using nonlinear characteristics of excitation and fluorescence emission when a fluorescence sample is excited by laser, and meanwhile, positioning information is obtained by surrounding a single molecule of a region of interest by using an illumination light beam; and the laser intensity is continuously improved, and the positioning precision is also improved until sub-five-nanometer precision information is obtained. And the detection end adopts a high-speed lock-in amplifier to lock fluorescence signal information of characteristic frequency in real time, and meanwhile, the final signal-to-background ratio is also improved. Compared with traditional high-precision positioning based on double laser light sources, the method has the advantages of a single laser light source, does not need special fluorescent dye, does not need special matching between double-wavelength lasers, and has universality; and the damage caused by phototoxicity and photobleaching due to over-high illumination intensity is avoided.

Compositions, testing chambers and methods for testing a fuel sample for microbial contamination (including fuels treated with a biocide) are provided, which comprise: a quantity of hydrocarbon fuel; a microbial contamination wherein the microbial contamination further comprises nucleic acid in the form of both DNA, RNA or a combination thereof, and an analyzing solution; wherein the analyzing solution comprises at least six (6) primer pairs for amplification of at least one target locus, wherein at least one primer of each pair of primers is labeled with a fluorescent dye and wherein at least one of the primer pair binds to the nucleic acid of the microbial contamination.

The invention discloses an image registration method and device and electronic equipment, and the method comprises the steps: obtaining multiple frames of target images, the target images being images obtained by carrying out image collection on a to-be-detected sample under laser irradiation, and the to-be-detected sample being dyed by a fluorescent dye; obtaining a target parameter corresponding to the target image, wherein the target parameter represents whether base deletion exists in the target image; according to the target parameters, the target image is divided to obtain a first image and a second image, the first image is an image without base deletion, and the second image is an image with base deletion; processing the first image by using an image registration algorithm to obtain a first registration result; and processing the second image according to the first registration result to obtain a second registration result.

The invention discloses a liquid-phase protein chip method for simultaneously detecting three kinds of porcine coronaviruses and a kit thereof, which comprises the following steps of: after activating and coupling microspheres, respectively coating antigen on porcine delta coronavirus N protein, porcine acute diarrhea syndrome coronavirus N protein and porcine epidemic diarrhea virus N protein by using the microspheres; matching the two fluorescent dyes according to different concentrations to form fluorescent coding microsphere combination arrays with different energy levels; the microspheres are analyzed one by one based on the flow principle, red laser recognizes microsphere codes, green laser reads fluorescence intensity of report molecules, computer analysis and processing are carried out, and fluorescence signals are converted into quantitative detection results; and determining the optimization of the detection conditions of the liquid-phase protein chip after the liquid-phase protein chip test. The antigens coated on the microspheres only specifically react with corresponding antibodies and are not combined with antibodies generated by other pathogens; the PDCoV, SADS-CoV and PEDV antibodies in the sample to be detected can be rapidly, effectively and accurately detected, and reference is provided for prevention of the porcine coronavirus.

In flow cytometry evaluation for unassociated virus-size particles stained with multiple fluorogenic dyes, the fluid sample is stained with an aqueous diluted dye formulation including multiple fluorogenic dyes. Preparation of the aqueous diluted dye formulation includes first preparatory processing to prepare a concentrated dye formulation with the fluorogenic dyes provided in a dry powder mixture mixed with and dissolved into a first liquid medium comprising DMSO. After the first preparatory processing, aqueous liquid diluent is added to dilute the first liquid medium and while the fluorogenic dyes remain in solution the liquid medium is converted to an aqueous liquid medium, which may include DMSO as a minor molar component but at a significant concentration. The stained fluid sample may include dissolved disaccharide.

The present application relates to the technical field of strength detection devices for casting parts, and specifically discloses a strength detection device for casting parts, which includes a detection table, on which a clamping assembly for fixing the casting parts, a pressure assembly for applying pressure, and a detection assembly for detecting whether the casting parts are plastically deformed are also arranged; the detection assembly includes an elastic capsule, a detection box, and a liquid storage tank, the detection box is fixed with a photosensitive sensor, the detection box is filled with a hydrogen peroxide solution, the liquid storage tank is filled with diphenyl oxalate mixed with a fluorescent dye, and the diphenyl oxalate in the liquid storage tank can be injected into the elastic capsule, so that the elastic capsule swells to fit the inner cavity of the casting parts; when the casting parts are plastically deformed, the diphenyl oxalate in the elastic capsule is squeezed into the detection box, so that it mixes with the hydrogen peroxide solution to produce fluorescence. The present application has the effect of improving the accuracy of strength detection, reducing the difficulty of detection operation, and improving detection efficiency.