513 results about "Fluorochrome Dye" patented technology

To provide a method for detecting the presence of multiple specific nucleic acid sequences or single nucleotide polymorphisms in a temperature-dependent fashion.SOLUTION: Provided is a method, in which an invasive oligonucleotide and a primary probe oligo (a 5' flap oligonucleotide) hybridize to a target strand nucleic acid to form an invasive cleavage structure cleavable by a FEN-1 endonuclease to release a cleaved 5' flap (primary cleaved flap); in a secondary reaction, the primary cleaved flap from the primary reaction hybridizes to a FRET cassette 1, a hairpin oligonucleotide labeled with a fluorescent dye and a quencher molecule, to form a second invasive cleavage structure that is cleavable by a FEN-1 endonuclease at a site between the fluorophore and a quencher; and cleavage of FRET cassette 1 separates the quencher from the fluorophore, such that fluorescence signal from the fluorophore can be detected.SELECTED DRAWING: Figure 1

There is proposed a method for detecting a presence of a fluorescence pattern type on an organ segment via immunofluorescence microscopy and digital image processing. The steps comprise: provision of the organ segment, incubation of the organ segment with a liquid patient sample, incubation of the organ segment with secondary antibodies which have been labelled with a fluorescent dye, acquisition of a fluorescence image of the organ segment in a colour channel corresponding to the fluorescent dye, and provision of the fluorescence image to a neural network. What takes place by means of the neural network is simultaneous determination of segmentation information through segmentation of the fluorescence image and, furthermore, of a measure of confidence indicating an actual presence of the fluorescence pattern type. What further takes place is determination, on the basis of the previously determined segmentation information, of at least one sub-area of the fluorescence image that is relevant to formation of the fluorescence pattern type, determination, on the basis of the previously determined at least one sub-area, of validity information indicating a degree of a validity of the measure of confidence, and output of the measure of confidence depending on the validity information.

Compounds useful as fluorescent or colored dyes are disclosed. The compounds have the following structure (I):or a stereoisomer, tautomer or salt thereof, wherein R1, R2, R3, R4, R5, L1, L2, L3, L4, M1, M2, m and n are as defined herein. Methods associated with preparation and use of such compounds is also provided.

Compounds useful as fluorescent or colored dyes are disclosed. The compounds have the following structure (I):or a stereoisomer, tautomer or salt thereof, wherein R1, R2, R3, R4, R5, L1, L2, L3, L4, M, m and n are as defined herein. Methods associated with preparation and use of such compounds are also provided.

The invention discloses an alanine aminotransferase detection device and method based on a liquid crystal microcavity array, and belongs to the field of optical sensing. The device comprises a functional liquid crystal microcavity array, an excitation optical fiber and a collection optical fiber, the microcavity array is composed of liquid crystal microcavities doped with fluorescent dye and stearic acid, and the multiple microcavities are independently distributed and serve as optical sensing units; the excitation optical fiber is used for synchronously exciting the microcavity to generate an echo wall mode, and the acquisition optical fiber acquires resonance spectrum signals of each channel in real time; the functionalized liquid crystal microcavity is converted into a bipolar configuration from a radial configuration in an enzymatic reaction, echo wall mode spectrum red shift is caused, spectrum shift time is in negative correlation with enzyme concentration, and dual-channel detection is realized by matching with conversion time of a polarized light pattern. According to the detection structure based on the liquid crystal microcavity array provided by the invention, high-flux, real-time and label-free rapid detection on the concentration of alanine aminotransferase can be realized.

Disclosed in the present invention is a stimulated emission depletion and super-resolution fluorescence lifetime imaging method, which is applied to the technical field of optical microscope imaging. The method provided in the present invention combines the stimulated emission depletion and fluorescence lifetime imaging principles, a depletion laser pulse frequency used therein is half of an excitation light pulse frequency, and a time interval between two pulse sequences is adjusted, such that a depletion laser pulse follows an adjacent excitation light pulse; and analysis processing is performed on collected fluorescence lifetime data, such that a super-resolution fluorescence lifetime image is obtained, thereby solving the problem of the fluorescence lifetime of a stimulated emission depletion and fluorescence lifetime imaging microscope being significantly shortened under the action of a high-energy depletion laser, and thus accurately reflecting real information of a fluorochrome and a micro-environment. Therefore, the innovative technique provides important technical support for life science research, significantly improves the accuracy and effectiveness of research, and promotes further development in the field of biomedicine.

The invention provides a method for measuring and calculating the article position of continuous silver ore with the particle size of 0.037 mm or above, and belongs to the technical field of mineral grade detection. The silver grade in the sample is measured and calculated through screening; leaching and measuring the content of silver monomers and continuous silver minerals, and reselecting to obtain a heavy sand product; carrying out fluorescent dyeing on the heavy sand product, and selecting monomer silver minerals; analyzing the color of the silver mineral through point scanning data of a scanning electron microscope, melting the monomer silver mineral to obtain the volume of the monomer silver mineral, and calculating the content of the monomer silver mineral; and carrying out conversion to obtain the continuous silver ore article position with the size of more than 0.037 mm. According to the method, the target mineral is rapidly and accurately obtained by adopting density medium reselection and fluorescent dye tracing; introducing a scanning electron microscope to measure and calculate the actual density of the silver mineral to replace the theoretical density, smelting the silver mineral in a mold, and carrying out volume measurement and calculation to obtain the actual metal amount closer to the actual silver mineral; measuring and calculating errors caused by impurities and irregular shapes of mineral particles are reduced; and the calculated grade of the silver mineral is more accurate.

The invention belongs to the technical field of plant fungus molecular biology detection, and discloses an LAMP primer group, a kit and a detection method for detecting oat smut pathogenic bacteria. A group of LAMP (loop-mediated isothermal amplification) specific primers are designed according to a specific sequence on a whole genome of the oat smut pathogenic bacteria Ustilago hordei, results are judged through a real-time fluorescence quantification method, an agarose gel electrophoresis method and an SYBR Green I fluorescent dye developing method, and the oat smut pathogenic bacteria carried by oat seeds are detected. The LAMP detection method for the oat smut pathogenic bacteria, established by the invention, is strong in specificity, high in sensitivity, high in speed and low in cost, provides a new technical means for detection of the oat smut pathogenic bacteria carried by the oat seeds, and has relatively high practical application value.

An optical sensor patch for measuring water activity is disclosed. The optical sensor patch includes a sheet configured to be in equilibrium with water activity in an environment of the optical sensor patch. A pair of distinct fluorescent dyes are dispersed within the sheet. The fluorescence spectra of the optical sensor patch is dependent on the water activity in the environment of the optical sensor patch. An optical implant and systems and methods for using the optical sensor patch are also disclosed.

The invention provides a benzobis (thiadiazole) near-infrared two-region fluorescent dye molecule and a preparation method thereof, and the preparation method comprises the following steps: by taking a benzobis (thiadiazole) derivative as a raw material, introducing 3, 4-ethylenedioxythiophene through a Stille cross-coupling reaction; the preparation method comprises the following steps: carrying out bromination with N-bromosuccinimide, introducing a cyanated dibenzocarbazole derivative through a Suzuki cross-coupling reaction, and finally carrying out reduction and cyclization reactions to synthesize the end cyano-functionalized near-infrared two-region fluorescent small molecular dye. The receptor-donor-receptor-donor-receptor (A '-D-A-D-A') structure near-infrared two-region fluorescence molecule disclosed by the invention has the properties of long absorption wavelength, high fluorescence quantum yield, high fluorescence brightness and the like, and has a wide application prospect in the field of near-infrared two-region fluorescence imaging.

The preparation method comprises the following steps: carrying out reflux reaction on 4-bromo-1, 8-naphthalic anhydride and primary amine in an organic solvent to obtain an intermediate product, and carrying out C-N coupling reaction on the intermediate product, a compound containing a nitrogen secondary amine electron-donating group, alkali and a catalyst in the organic solvent to obtain the double-state emission fluorescent dye. The two-state emission dye is obtained. An amino auxochrome group of a bridge ring structure is modified in a 4-bromine-1, 8-naphthalimide parent, the fluorescence quantum yield of the dye in a polar solvent is improved through the characteristic that nitrogen atom lone pair electrons of the nitrogen-containing bridge ring structure are not easy to overturn, and pi-pi stacking, which is not beneficial to emission, of the dye is inhibited through the large steric hindrance effect of the bridge ring structure; the dye shows bright fluorescence emission in non-polar and polar solvents, also has a very strong fluorescence quantum yield in a solid state, and shows a quantum yield greater than 94% when the dye is dispersed in an EVA (Ethylene-Vinyl Acetate) film.

The invention discloses an STR (short tandem repeat) fluorescence labeling composite amplification system, the STR fluorescence labeling composite amplification system comprises 6 STR loci on a human chromosome 15, 12 STR loci on a human chromosome 20 and corresponding 36 primer pairs, and each primer pair carries a fluorescent dye marker. The kit can be used as an effective supplement of an existing kit, the false positive rate can be remarkably reduced, the detection accuracy and reliability are effectively improved, an important basis is provided for prenatal diagnosis and genetic disease detection, and the kit has important clinical application value and wide market prospects.

In one embodiment, a method is disclosed to determine one or more biological cells of interest to identify and count in a mixed biological sample fluid with differing biological cells. The method can include selecting cell markers associated for biological cells to which conjugated antibodies can attach with differing fluorescent dyes; displaying a panel builder graphical user interface window to display a co-expression matrix by biological cell type to assist in selecting cell markers to assign co-expression; and selecting cell markers to assign co-expression with an input device.

The invention discloses a light capture system based on a polybenzyl ether dendrimer as well as a preparation method and application of the light capture system. According to the light capture system, a core mono-cyano ethylene modified polybenzyl ether dendrimer is used as a light acquisition antenna and an energy donor, and a fluorescent dye is used as an energy receptor; the core mono-cyano ethylene modified polybenzyl ether dendrimer and the fluorescent dye are co-assembled in a solution to form a light capture system. The light capture system provided by the invention has excellent aggregation-induced emission performance, obviously enhanced fluorescence and narrower emission band, and can still maintain better energy transfer and ultrahigh antenna effect under a high donor-acceptor ratio; in addition, the preparation process is green and environment-friendly, and the light capture system is stable in structure and can be stored for a long time.

Compositions, testing chambers and methods for testing a fuel sample for microbial contamination (including fuels treated with a biocide) are provided, which comprise: a quantity of hydrocarbon fuel; a microbial contamination wherein the microbial contamination further comprises nucleic acid in the form of both DNA, RNA or a combination thereof, and an analyzing solution; wherein the analyzing solution comprises at least six (6) primer pairs for amplification of at least one target locus, wherein at least one primer of each pair of primers is labeled with a fluorescent dye and wherein at least one of the primer pair binds to the nucleic acid of the microbial contamination.

The invention discloses a liquid-phase protein chip method for simultaneously detecting three kinds of porcine coronaviruses and a kit thereof, which comprises the following steps of: after activating and coupling microspheres, respectively coating antigen on porcine delta coronavirus N protein, porcine acute diarrhea syndrome coronavirus N protein and porcine epidemic diarrhea virus N protein by using the microspheres; matching the two fluorescent dyes according to different concentrations to form fluorescent coding microsphere combination arrays with different energy levels; the microspheres are analyzed one by one based on the flow principle, red laser recognizes microsphere codes, green laser reads fluorescence intensity of report molecules, computer analysis and processing are carried out, and fluorescence signals are converted into quantitative detection results; and determining the optimization of the detection conditions of the liquid-phase protein chip after the liquid-phase protein chip test. The antigens coated on the microspheres only specifically react with corresponding antibodies and are not combined with antibodies generated by other pathogens; the PDCoV, SADS-CoV and PEDV antibodies in the sample to be detected can be rapidly, effectively and accurately detected, and reference is provided for prevention of the porcine coronavirus.

The present application relates to the technical field of strength detection devices for casting parts, and specifically discloses a strength detection device for casting parts, which includes a detection table, on which a clamping assembly for fixing the casting parts, a pressure assembly for applying pressure, and a detection assembly for detecting whether the casting parts are plastically deformed are also arranged; the detection assembly includes an elastic capsule, a detection box, and a liquid storage tank, the detection box is fixed with a photosensitive sensor, the detection box is filled with a hydrogen peroxide solution, the liquid storage tank is filled with diphenyl oxalate mixed with a fluorescent dye, and the diphenyl oxalate in the liquid storage tank can be injected into the elastic capsule, so that the elastic capsule swells to fit the inner cavity of the casting parts; when the casting parts are plastically deformed, the diphenyl oxalate in the elastic capsule is squeezed into the detection box, so that it mixes with the hydrogen peroxide solution to produce fluorescence. The present application has the effect of improving the accuracy of strength detection, reducing the difficulty of detection operation, and improving detection efficiency.

The invention relates to a method for detecting miRNA (micro Ribonucleic Acid) by using a ratio-type CRISPR / Cas12a (Clustered Regularly Interspaced Short Palindromic Repeats / CRISPR / Cas12a) system. The method is characterized by being realized by applying a multifunctional probe in the CRISPR / Cas12a system. Wherein the multifunctional probe is a single-stranded DNA (deoxyribonucleic acid) with a hairpin structure and comprises two functional areas, namely a neck part and a loop part; wherein the neck part of the probe is used for identifying a target miRNA; wherein the probe ring part has various different functions of lightening a fluorescent dye, activating a CRISPR / Cas12a system, serving as a Cas12a trans-cracking substrate, triggering a self-driven cascade reaction and the like; the working principle of the method is as follows: when no target exists, the ring part of the probe cannot activate the CRISPR / Cas12a system, the G quadruplex structure of the ring part of the probe can be combined with a fluorescent dye to emit fluorescence, and the neck part of the probe is opened by combining the sticky end with the target when the target exists, so that the G quadruplex structure of the ring part of the probe disappears and releases an activator to activate the CRISPR / Cas12a system; the activated Cas12a simultaneously reversely cuts the reporter probe and the multifunctional probe around the Cas12a, so that the fluorescence of the reporter probe is enhanced and the fluorescence of the multifunctional probe is reduced, and the multifunctional probe releases more activators to trigger a self-driven cascade reaction, so that the fluorescence of the reporter probe is further enhanced and the fluorescence of the multifunctional probe is further reduced; quantitative detection of the target miRNA can be realized through the mathematical relationship between the fluorescence ratio of the report probe and the multifunctional probe and the target concentration. The ratio-type CRISPR / Cas12a system is designed according to the structure activity of DNA, the property of the fluorescent dye and the characteristics of the CRISPR / Cas12a system, sensitive detection of miRNA is achieved, and the ratio-type CRISPR / Cas12a system can be used for scientific research analysis and clinical detection and has wide market prospects.

The invention relates to an endoscope imaging system and a light source thereof, the light source comprises a laser light source, a visible light source and a light combiner, the laser light source is used for outputting laser of at least two different wavebands, the laser of the different wavebands is used for exciting different fluorescent dyes, and the visible light source is used for outputting visible light; the light combiner is used for combining the laser and the visible light into a combined light beam and outputting the combined light beam to the endoscope; wherein the laser light source is a single light-emitting device, the single light-emitting device comprises a single substrate and at least two laser chips arranged on the single substrate, the at least two laser chips are used for emitting laser of different wave bands, and the single light-emitting device further comprises a single light outlet and a single optical fiber; and laser emitted by the at least two laser chips is output to the single optical fiber through the single light outlet. The light source can independently or simultaneously output laser of different wave bands to excite different fluorescent dyes, and the layout of an endoscope imaging system is simplified by adopting the light source.

A method of medical multi-dye fluorescence imaging. The method including: capturing fluorescent images of an operating field in which two or more different fluorescent dyes are present in successive sequences of images with a surgical or diagnostic imaging device, each sequence of images alternating through different modes of excitation lighting adapted for at least one each of two or more of the two or more different dyes, and processing the captured images of each successive sequence of images in combination with each other. The processing comprising determining the respective local distributions of the two or more different fluorescence dyes causing the observed different distributions of brightness in the two or more different fluorescent images of the sequence and producing one or more images displaying the determined local distributions of the two or more different fluorescent dyes separately.

The invention provides a qPCR (quantitative polymerase chain reaction) method for detecting baculovirus plasmid recombination rate. The qPCR method comprises the following steps: designing a total baculovirus primer; designing a recombinant stem grain primer; the qPCR detection method comprises the following steps: adopting a fluorescent dye qPCR method, taking the extracted stem grain DNA as a template, respectively detecting the same template by using the total stem grain primer pair and the recombinant stem grain primer pair to obtain the Ct total value of the total stem grain and the Ct weight value of the recombinant stem grain, obtaining the difference value delta Ct = Ct total-Ct weight, and calculating the recombination ratio% = 2 delta Ct * 100%. The invention provides a novel method for detecting the proportion of recombinant rods, the total rod primer pairs Bacmid-F and Bacmid-R can specifically amplify non-recombinant rods and recombinant rods, and the recombinant rod primer pairs Tn7L-F and M13-R can only amplify a sample containing the recombinant rods, so that the proportion of the recombinant rods in the sample can be detected by adopting a qPCR (quantitative polymerase chain reaction) method; the recombination proportion can be used for guiding subsequent experiments.

The invention discloses a near-infrared organic fluorescent dye with mechanical responsiveness, and relates to the technical field of organic fluorescent dyes, the near-infrared organic fluorescent dye with mechanical responsiveness is prepared through seven-step reaction, an intermediate M1 containing a hydroxyl active end is firstly prepared, then intermediates M2, M3 and M4 are obtained through multi-step reaction, a target dye is obtained after purification, and finally surface functionalized nano-particles are self-assembled, so that the near-infrared organic fluorescent dye with mechanical responsiveness is obtained. The preparation method has the advantages that by designing a conjugate regulation and control structure of a rigid chromophore core and a flexible bridging unit, a mechanical response group is directionally grafted to the tail end of the flexible bridging unit through a CuAAC click chemical reaction; the problems of low fluorescence quantum yield and high response threshold caused by conjugation conflict of a traditional mechanical response group and a near-infrared chromophore are solved, the mechanical response threshold is reduced to a low stress range required by biomechanical detection, collaborative optimization of mechanical responsiveness and near-infrared fluorescence performance is realized, and the method is suitable for the fields of high-sensitivity biological stress monitoring and the like.

The invention discloses a fluorescent dye for shoe soles as well as a preparation method and application of the fluorescent dye, and belongs to the technical field of fluorescent materials for shoes. The fluorescent dye has a structure as shown in a formula 1. The preparation method comprises the following steps: synthesizing an intermediate through Buchwald-Hartwisting arylation reaction, and carrying out Suzuki coupling to obtain a target product. The dye inhibits aggregation fluorescence quenching through a rigid plane structure and a deuterated group, the HOMO-LUMO energy gap is regulated to 2.8-3.2 eV, the dye has the broad band emission of 450-600 nm, and the fluorescence quantum efficiency reaches 0.55-0.75. When the brominated butyl rubber is applied to a sole formula, the brominated butyl rubber comprises the following components in parts by weight: 40-60 parts of brominated butyl rubber, 0.1-2 parts of fluorescent dye and the like, and a finished product is prepared by mixing, vulcanizing and foaming. Compared with a traditional dye, the fluorescence intensity is improved, the photooxidation resistance is enhanced, the dissolution rate is reduced by 60% or above, and the dye has excellent humidity and heat resistance and material compatibility.

The invention relates to the technical field of immunochromatography, in particular to a TSYl modified magnetic time-resolved fluorescent microsphere as well as a preparation method and application thereof, and the preparation method comprises the following steps: 1) preparing a surface aminated Fe3O4 magnetic core by a hydrothermal synthesis method; (2) carrying out double-bond modification on the surface of Fe3O4 through nucleophilic substitution reaction; 3) preparing surface hydroxyl modified polymer magnetic microspheres by a seed emulsion polymerization method; 4) through nucleophilic substitution reaction, performing tosyl modification on the surface of the magnetic microsphere; 5) preparing a time-resolved fluorescent dye Eu (III) complex; and 6) embedding a rare earth complex into the microspheres by a swelling method to prepare the surface tosyl modified magnetic time-resolved fluorescent microspheres. The TSYl modified magnetic time-resolved fluorescent microspheres prepared by the method have the advantages that the particle size range is 200-400nm, the particle size is uniform, and the monodispersity is good; the antibody can be directly combined with protein / antibody without activation; good magnetic responsiveness and fluorescence intensity are realized.

The invention discloses a full-automatic multiplex fluorescent staining detection method for EBER, CD3 and CD20 and application of the full-automatic multiplex fluorescent staining detection method, and belongs to the technical field of biomarker detection. The invention relates to an EBER, CD3 and CD20 detection method, which comprises: carrying out digestion treatment on a sample to be detected, adding an EBER probe, and sequentially adding a primary antibody, a secondary antibody and a fluorescent dye A so as to complete color development; sequentially adding a CD3 antibody, an HRP polymer and a fluorescent dye B into the to-be-detected sample to finish CD3 dyeing; sequentially adding a CD20 antibody, an HRP polymer, a fluorescent dye C and a DAPI dye into a sample to be detected to finish CD20 dyeing; and observing or counting the dyeing result of the to-be-detected sample through fluorescence scanning equipment.

In one embodiment, a method of building an optimized color flow cytometry panel is disclosed using a full spectrum flow cytometer with five excitation lasers and five corresponding detection modules. In another embodiment, a graphical user interface is disclosed generated by a server computer from a fluorochrome database and displayed by a client computer to assist in the selection of a set of fluorochromes for use in an assay to analyze biological samples. The GUI can display spectra graphs to visually show how fluorochromes may overlap and can generate similarity indexes for the paired fluorochrome interference and a complexity index for overall many to many interferences generated by a selected group or set of fluorochromes.

Compositions, testing chambers and methods for testing a fuel sample for microbial contamination (including fuels treated with a biocide) are provided, which comprise: a quantity of hydrocarbon fuel; a microbial contamination wherein the microbial contamination further comprises nucleic acid in the form of both DNA, RNA or a combination thereof, and an analyzing solution; wherein the analyzing solution comprises at least six (6) primer pairs for amplification of at least one target locus, wherein at least one primer of each pair of primers is labeled with a fluorescent dye and wherein at least one of the primer pair binds to the nucleic acid of the microbial contamination.

The present application relates to the technical field of molecular biology detection, in particular to an absolute antibody Taq DNA polymerase activity determination method based on real-time fluorescence quantitative detection, comprising the following steps: firstly, establishing a standard curve of DNA content and fluorescence value by reacting different concentrations of double-stranded lambda DNA with fluorescent dyes; then, performing an extension reaction in a specific reaction system using M13 single-stranded DNA as a template, recording fluorescence values in real time, calculating net fluorescence values and establishing a linear relationship between the net fluorescence values and reaction time; converting the amount of newly generated double-stranded DNA at different time points by using the standard curve, further calculating the consumption amount of dNTPs, and finally obtaining the absolute activity of the antibody Taq DNA polymerase according to the definition of the activity of the antibody Taq DNA polymerase. The whole process combines fluorescence detection and a standard curve to realize accurate determination of enzyme activity.

The invention relates to the technical field of sensing of magnetic nano photoelectric materials, in particular to a responsive microsphere based on a three-dimensional magnetic photonic crystal and a preparation method and application thereof, and the responsive microsphere is formed by arranging polyhydroxy compound modified magnetic nanoparticles in a spherical polymer into a non-close packing face-centered cubic or body-centered cubic structure. The responsive microsphere based on the three-dimensional magnetic photonic crystal is easy in color distinguishing, has no angle dependence, does not need to depend on a fluorescent dye or a radioactive label, can realize real-time and in-situ monitoring through own optical characteristics, breaks through the bottlenecks of sensitivity and resolution of a traditional sensor, realizes visual detection, simplifies the detection process, and is suitable for mass production. The cost is reduced. According to the responsive microsphere based on the three-dimensional magnetic photonic crystal, the substance diffusion path is shortened through the small size of the responsive microsphere, and the response speed is remarkably increased in combination with the high specific surface area.

The invention discloses a hemicyanine fluorescent dye-based compound as well as a preparation method and application thereof. The invention provides a compound MCYA based on hemicyanine fluorescent dye targeting AChE, the compound has high sensitivity to AChE, the detection limit can be as low as 0.0225 U / mL, and high-sensitivity detection on AChE can be realized; and the MCYA has good selectivity and strong anti-interference capability on common ions and amino acids in a biological system. A fluorescence sensor platform built by combining MCYA and AChE can realize qualitative and quantitative analysis of organophosphorus and carbamate pesticide residues, and has the advantages of high sensitivity, simplicity in operation, low cost, short detection time, suitability for rapid detection in the market and the like.