1109 results about "Living cell" patented technology

The invention provides an enhancer RNA (Ribonucleic Acid) molecule MZGAe1 and application thereof, and relates to the technical field of biology. The invention provides an enhancer RNA (Ribonucleic Acid) molecule MZGAe1. The nucleotide sequence of the enhancer RNA molecule MZGAe1 is as shown in SEQ ID NO: 1; meanwhile, the invention further provides a specific sgRNA sequence for activating the molecule in a targeted manner and a recombinant vector of the specific sgRNA sequence. Expression of endogenous MZGAe1 of cells is specifically activated by adopting a CRISPR activation technology, and conversion of mouse embryonic stem cells to bicellular-like cells can be efficiently promoted. By providing a brand-new endogenous targeted MZGAe1 accurate activation tool, the efficient transformation of wild mouse embryonic stem cells to bicellular cells can be realized only under the condition of endogenous activation of single enhancer RNA molecule MZGAe1, the proportion is at least increased by 5%, the expression of a totipotent marker gene ZSCAN4 is activated, and the expression of the totipotent marker gene ZSCAN4 is promoted. And an efficient and specific brand-new research tool and scheme are provided for researching zygote genome activation, embryonic development early events and cell reprogramming.

The invention relates to a culture method for activating NKT cells by DC cells in vitro and application of the NKT cells. The preparation method of the NKT cell comprises the following steps: activating a DC cell by using cell inducing factors such as alpha-GalCer, GM-CSF and IL-4, co-culturing the DC cell and a cryopreserved and resuscitated suspension cell left after adherent sorting, and activating and amplifying the NKT cell. The proportion of NKT (CD3 + CD56 +) prepared by the invention is more than 80%, and a small amount of iNKT cells are contained at the same time.

The invention relates to the technical field of fluorescent dyes, in particular to a citric acid-based fluorescent dye for distinguishing dead cells from living cells as well as a preparation method and application of the citric acid-based fluorescent dye. The invention discloses a citric acid-based fluorescent dye for distinguishing dead cells from living cells. The citric acid-based fluorescent dye comprises at least one of a compound 1 and a compound 2, the citric acid-based fluorescent dye can specifically enter dead cells and specifically dye cytoplasm of the dead cells, so that the dead cells can be dyed or imaged. The citric acid-based fluorescent dye is difficult to dye living cells, so that dead cells and living cells can be quickly and accurately distinguished, and the citric acid-based fluorescent dye can be applied to the field of biological medicines.

KLRB1 binding agents (in particular anti-KLRB1-antibodies and antigen binding portion thereof) with increased humanness and compositions thereof, as well as therapeutic methods of using the agents, e.g., for depleting cells or inhibiting cells or activating cells, (in particular, Th17, Th17.1, ex-Th17, Tc17, MAIT, iNKT, peTh2, ILC2, ILC3, NK cells, and / or neoplastic T or NK cells in vivo), for the treatment of autoimmune disease, allergic diseases, transplant rejection, hematologic malignancies, and cancer.

The invention belongs to the technical field of microorganisms and fermentation engineering, and particularly relates to application of bifidobacterium longum subsp. Infantis Y46 in the aspects of enhancing cell defense and repair and regulating metabolic homeostasis of a host. The strain is isolated from infant feces. Animal experiments prove that living cell thalli of the bifidobacterium longum subsp. Infantis Y46, which are obtained by culturing an MRS liquid culture medium added with L-cysteine hydrochloride (0.05%, v / v), can significantly enhance the oxidative stress resistance of an organism and reduce the level of active oxygen in the organism compared with a control bacterium bifidobacterium longum subsp. Infantis 15697. Transcriptomics analysis shows that the bifidobacterium longum subsp. Infantis Y46 can enhance self-protection of cells and improve physiological imbalance caused by external pressure by regulating related signal channels of an organism. The action mechanism is closely related to signaling pathways related to cell defense, metabolic homeostasis and barrier functions. The invention provides a new strain resource and an application scheme for developing functional food or medicinal preparations for enhancing the health defense function of a human body.

The invention belongs to the technical field of microorganisms and fermentation engineering, and particularly relates to application of bifidobacterium longum subsp. Infantis Y46 in the anti-aging aspect. The bifidobacterium longum subsp. Infantis Y46 is obtained from excrement of infants. Animal experiments prove that living cell thalli (Y46) and thermally inactivated cell thalli (HKY46) obtained by culturing the bifidobacterium longum subsp. Infantis Y46 by using an MRS liquid culture medium containing L-cysteine hydrochloride (0.05%, v / v) can significantly reduce accumulation of lipofuscin in a host, and prolong the healthy life and life of the host. Therefore, the bifidobacterium longum subsp. Infantis Y46 is a promising probiotic and a promising metagen with an anti-aging function.

The invention discloses a living cell super-resolution imaging method and system based on self-supervised learning. The method comprises the following steps: S1, obtaining a fluorescence microscopic image through a standard fluorescence microscopic system; s2, for the acquired single noise image, generating positive and negative sample pairs of self-supervised training data through an autonomously designed and optimized self-supervised strategy; s3, constructing a front denoising neural network and a rear deconvolution network, and performing network training; and S4, inputting a newly obtained noise image into the trained front denoising network to obtain a denoised image, and inputting the denoised image into the rear deconvolution network to obtain a final super-resolution reconstructed image. According to the invention, the standard fluorescence microscopic system and the self-supervised denoising processing module are combined, so that high-quality denoising and super-resolution reconstruction of the fluorescence microscopic image can be realized in a low-photon signal scene.

The invention relates to artificial skin, in particular to self-generating full-layer artificial skin for wound treatment and application of the self-generating full-layer artificial skin. The artificial epidermis has an epidermis-dermis structure similar to skin, and the artificial epidermis structure is composed of an electrostatic spinning nano-film with piezoelectric capacity and has the functions of sterilization, protection and bioelectricity generation of an epidermis layer; the artificial dermal structure is composed of a photo-crosslinking polysaccharide and enzyme crosslinking protein interpenetrating hydrogel skeleton, by adding bioactive components and loading living cells, the effects of releasing active components and cytokines, remodeling wound extracellular matrix and promoting cell proliferation are achieved, and finally healing of acute and chronic wounds is remarkably accelerated. The invention provides a simple, feasible, economical and effective treatment method for sealing a large-area wound, sterilizing the wound and promoting healing of the wound.

The invention discloses an organelle-targeted fluorescent probe for specific detection of tumor cells as well as preparation and application of the organelle-targeted fluorescent probe, and the specific structural formula of the fluorescent probe is as follows: BCY-DP shows obvious specific targeting ability of tumor cell mitochondria and has been applied to ONOO-selective imaging in living cell mitochondria; results show that the BCY-DP has high selectivity on ONOO <-> in tumor cells, can successfully distinguish the ovarian cancer cells from normal ovarian cells, and can be used for diagnosis of cancer cells.

The present invention relates to a method for in vitro sorting of target cells for cell therapy, belonging to the technical field of cell therapy. The present invention utilizes a washing solution containing EGTA, EDTA, α-casein, and prostaglandins to achieve a sorting method with high viable cell recovery efficiency, thereby enabling better cell therapy.

A method of providing a channel in nervous tissue filled with an aqueous gel for implantation of a microelectrode or other medical device lacking sufficient physical stability for direct implantation by insertion, comprises providing an apparatus comprising an oblong rigid pin covered by a dry gel forming agent; locating a target in the tissue; defining a straight insertion path a desired tissue insertion point and the target; aligning the pin with its end foremost with the insertion path; inserting the pin into the tissue to a position near or at the target; allowing sufficient time to pass for a gel to be formed around the pin, withdrawing the pin. Also disclosed is a corresponding channel; a method of implantation of a microelectrode or microprobe into nervous tissue via the channel; a corresponding method of implantation of living cells; a corresponding apparatus for forming the channel.

The present invention relates to compositions for in vitro and in vivo delivery of nucleic acids, in particular messenger RNAs (mRNAs), into a target cell and their applications. The present invention is directed to a composition comprising (A) at least one nucleic acid and (B) at least one lipid nanoparticle (LNP) comprising (i) at least one ionizable lipid; (ii) at least one phospholipid; (iii) at least one sterol, especially neutral sterol; (iv) at least one poly(ethyleneglycol)-lipid (PEG-lipid); and (v) an imidazolium-based cationic lipid of formula (I), wherein R1, R2, R3, R4, R5, R6, R7, R8 and R9, Y and A− are as defined in the description. The present invention also relates to a method for in vitro or in vivo transfection of live cells and uses of said composition.

Embodiments are directed to N3-kethoxal reagents and derivatives thereof, and related methods that allow fast and reversible labeling of single-stranded nucleic acids in live cells. By way of example, one aspect is directed to a process for reversible labeling of single-stranded guanine bases in live cells, which results in an effective in vivo method for transcriptome-wide RNA secondary structure mapping and RNA G-quadruplex prediction.

The invention discloses a drug delivery system based on branched chain nucleic acid as well as a preparation method and application of the drug delivery system. The drug delivery system comprises a multivalent nucleic acid framework, a nucleic acid-small molecule coupling drug and a nucleic acid-polypeptide coupling drug, a nucleic acid nanostructure is formed through base complementary pairing assembly, and the nucleic acid-small molecule coupling drug comprises first nucleic acid and an E3 ubiquitin ligase ligand coupled with the first nucleic acid; the nucleic acid-polypeptide coupling drug comprises a second nucleic acid and a cell penetrating peptide coupled with the second nucleic acid. The drug delivery system disclosed by the invention can respond to a tumor microenvironment, so that cell penetrating peptides are activated, tumor penetrating and cytoplasm delivery capacities are enhanced, the aim of efficiently degrading tumor-related target proteins is fulfilled by combining a multivalent effect, and a remarkable tumor treatment effect is achieved; in addition, the preparation method is simple and easy to implement, has universality and high production efficiency, and is expected to be applied to treatment of various tumors.

The invention discloses a nanoparticle scattered light confocal imaging device and method. The device comprises a laser unit, a first pinhole, a beam splitter turntable, a scanning focusing unit, an electric objective table, a first detection imaging unit, a second pinhole, a light splitting unit and a second detection imaging unit. The method does not need to mark nano particles, and can synchronously collect unmarked nano particles and fluorescence-labeled biomolecules; in-situ imaging can be realized, and the distribution position and content of nano-particles in living cells can be directly observed; the imaging resolution ratio is high, single nanoparticle imaging can be carried out, and fluorescence imaging can also be carried out at the biomolecule level.

The present invention relates to 4-1BB ligand (4-BBL) extracellular domain (ECD) muteins having reduce affinity for its cognate receptor 4-1BB. The 4-1BBL ECD muteins can be present in homo- or heterotrimeric fusion protein comprising three 4-1BBL ECD monomers. The invention further relates conjugates of such the 4-BBL ECD muteins with a heterologous moiety, such as an antigen binding protein. The antigen-binding regions comprised in the antigen binding protein in the conjugates preferably are specific for a tumor-associated antigen (TAA). In addition to a 4-BBL ECD mutein, the conjugates can comprise further NK cell-activating cytokines, such an IL-21 receptor agonist. The conjugates can further comprise an antigen-binding region that has affinity for a surface antigen expressed on NK cells, e.g. CD16A. Alternatively, the conjugates can further comprise an antigen-binding region that specifically binds an epitope of a γδ TCR. The conjugates of the invention specifically redirect and activate NK cells or γδ T cells to lyse targeted tumor cells. The invention further relates to the use of the 4-BBL ECD muteins and conjugates thereof in the treatment of cancer, preferably a cancer expressing the TAA.

The invention relates to a Raman activated cell sorting system coupled with micro-fluidic and Raman optical tweezers and a method thereof, and relates to the technical field of cell sorting, the system comprises a micro-fluidic chip with low Raman and fluorescence background noise and a pneumatic micro-valve; in the micro-fluidic chip, a sample channel and a micro-cavity are connected with a main channel, and a flow guide channel is connected with the micro-cavity; the flow guide channel and the collecting channel are connected through a pneumatic micro valve. After the pneumatic micro valve is closed, the fluid in the micro cavity is static, and a narrow sample flow is formed in the main channel. And when the Raman collection time is less than the time difference of two adjacent cells flowing through the capturing point of the optical tweezers in the narrow sample flow, high-flux Raman detection can be performed in the narrow sample flow. On the contrary, the target cells can be moved out of the narrow sample flow, and high-precision Raman detection in a long Raman collection scene can be realized. After sorting is completed, fluid flowing of the main channel is stopped, the pneumatic micro valve is opened, the cells stored in the micro cavity are released and collected in a unified mode, the cell suspension volume is more stable and uniform, and follow-up experiment operation is facilitated.

The present disclosure provides antibodies that specifically bind to TIGIT. The antibodies have the capacity for substantial activation of T cells and natural killer cells by inhibiting binding of TIGIT to CD155. The antibodies can be used for treatment of cancer and infectious disease, among other applications.

The invention discloses a vesicle transportation path prediction system based on artificial intelligence, and relates to the technical field of vesicle transportation, which comprises the following steps of: accurately extracting a complete time sequence movement track of a single vesicle before and after disturbance by virtue of fluorescence living cell imaging under a disturbance experiment condition in combination with a target detection model YOLOv8 and a depth correlation tracking algorithm DeepSORT; the problem that perturbation conditions are insufficiently perceived is solved, through a multi-modal data matrix, the model has the time sequence perceiving capacity for changes of a local space structure where vesicles are located, so that the sensitivity and modeling capacity of the model for track variation in a complex environment are remarkably improved, deep fusion of multi-modal features is achieved by building a double-branch fusion model, and the accuracy of the model is improved. The problem that an existing prediction model cannot actively recognize and correct prediction deviation is effectively solved, and the stability and the actual usability of the system are improved.

The invention belongs to the technical field of cellular immunity, and particularly relates to calcium-doped manganese phosphate engineered red blood cells based on a biomimetic mineralization technology and application of the calcium-doped manganese phosphate engineered red blood cells in immunotherapy. Calcium ions, manganese phosphate, acidic polypeptide and a carboxyl activator are deposited on the surfaces of red blood cells, the acidic polypeptide provides a negative electricity environment, the concentration of calcium and manganese on red blood cell membranes is increased, and a mineralization layer of calcium ions and manganese ions is formed; the carboxyl activator can activate carboxyl of amino acid on acidic polypeptide and is covalently bound with other molecules; calcium can improve the stability of the manganese phosphate crystal, optimize the release of manganese ions and activate a cGAS-STING pathway in the DC; the natural half-life period of red blood cells reaches 120 days, rapid clearing of the liver can be avoided, the aged red blood cells are swallowed by spleen DC, and cross presentation of antigens is promoted. The calcium-doped manganese phosphate engineered erythrocyte provided by the invention can promote the maturation, migration, homing and antigen presentation functions of dendritic cells, and enhance the ability of the dendritic cells to activate CD8 + T cells.

The invention discloses a nucleic acid tetrahedron nanostructure as well as a preparation method and application thereof. The nucleic acid tetrahedron nanostructure is composed of circRNA for coding an antigen, an ISD chain and two specific single-stranded DNA sequences, is simple and easy to prepare, can enter cells through cell membrane microfossa mediated endocytosis without the help of a transfection reagent, and is positioned to lysosome. The nanostructure is split to release circRNA coding antigen protein, and the circRNA coding antigen protein is delivered to APC and ISD chains in drainage lymph nodes (dLN) to activate cGAS-STING pathways, so that antigen presentation and immune activation are synergistically enhanced, T cells are efficiently and continuously activated, and the effect of tumor immunotherapy is achieved.

This disclosure is directed generally to wireless communications and more specifically to an improved handling of mobility of wireless terminals via successive / subsequent conditional cell change. The various example implementations are provided to describe example manners for preparing candidate SCG / PSCell configurations for SCG selective activation to support subsequent conditional PSCell addition / change among various network elements, including indicating the available / subsequent candidate PSCells among all candidate PSCells to be evaluated when a candidate PSCell become a serving PSCell during the UE mobility.

The invention discloses a pHi probe based on living cell incubation as well as a preparation method and application of the pHi probe. The preparation method comprises the following steps: drawing a capillary tube into a nano suction tube with the inner diameter of 120 nm; inserting the prepared nano-pipette into cells, incubating for a period of time, then washing the nano-pipette with ultrapure water, then putting the nano-pipette into a phosphate buffer solution (PBS) with phosphate radicals removed, and preserving, so as to obtain the pHi probe based on living cell incubation after the preservation is finished. According to the preparation method of the pHi probe disclosed by the invention, the pHi probe is prepared by inserting the nano suction tube into the cell, and the pHi probe can be prepared by inserting the nano suction tube into the cell and incubating for 60 minutes. The preparation method is simple, efficient and low in dependence on equipment, the prepared nanopipette pHi probe is sensitive to pH response, and the dynamic change of single cell pHi can be monitored in real time.

A plaque detection method and apparatus wherein at least one processor is programmed to receive above focus images to detect the presence of live cells without detecting the lysed cell materials, receive below focus images wherein virtual dark regions exist which are similar to cell shadows as seeds in a segmentation process and use contours around each resulting shape to obtain a subset that are more likely to be part of the cell population to define a cell map. A distance map is created in which each pixel value is the distance of that pixel from the nearest pixel of the cell map and the distance map is thresholded to create a first image of the places which are relatively far from the cells a second image with a smaller distance threshold to get an image that mimics the edges of the cells.

The present disclosure relates to methods of determining percentage viability of a cell composition comprising cell aggregates. Such methods may include providing a first aliquot of cells and a second aliquot of cells, wherein the first aliquot of cells and the second aliquot of cells have about the same concentration of cells, measuring a total number of live cells in the first aliquot of cells; measuring a total number of cells in the second aliquot of cells, and determining the percentage viability of the cell composition as a ratio between the total number of live cells in the first aliquot of cells and the total number cells in the second aliquot of cells.

The invention discloses a near-infrared fluorescent probe based on a xanthene-quinoline conjugated skeleton for detecting Cu < 2 + > as well as a preparation method and bioimaging application of the near-infrared fluorescent probe. The molecular formula of the fluorescent probe is C34H32N4O2. Based on the strategy of inhibiting the PET process, the large Stokes shift near-infrared fluorescent probe for specifically recognizing Cu < 2 + > is constructed, and spectrum experiments show that the probe has excellent recognition performance on Cu < 2 + >, the emission wavelength is 720 nm, and the Stokes shift reaches 170 nm; (2) the specificity is high, and Cu < 2 + > and other biological related metal ions can be effectively distinguished; (3) low detection limit (LOD = 17.6 nM); (4) quick response dynamics (lt; 30s) for a motor vehicle. More importantly, the probe XQ-NH2 is successfully applied to Cu < 2 + > imaging in living cells and living mice, it is proved that XQ-NH2 not only can provide a reliable technical means for Cu < 2 + > detection and imaging research in a complex biological system, but also shows that XQ-NH2 has important application potential in related fields.

The invention discloses a rapid biological cell classification imaging method and system based on a quantum correlation diffraction optical neural network, and the method comprises the steps: regulating and controlling a signal photon to an orbital angular momentum state optimized by a neural network based on a time-energy correlation two-photon pair generated in a spontaneous parametric down-conversion process, and irradiating a biological cell sample; a diffractive optical neural network is utilized to construct a quantum state illumination diffractive optical processor, photons carrying cell complex amplitude information are mapped to different detection areas of a predefined image plane, and rapid and high-precision cell classification and recognition are realized through correlation imaging results of an enhanced charge coupled camera. The method has the signal-to-noise ratio and anti-interference capability exceeding the classic limit, can keep high-precision cell classification capability under high background noise, and is suitable for long-time monitoring of living cells under the condition of extremely low light intensity. The method has the characteristics of low energy consumption and high efficiency, and meets the requirements of green calculation and sustainable development. And the method can be used for rapidly classifying and identifying the living biological cells without marks and phototoxicity.

According to the living cell observation device, the observation system and the observation method, a light beam generated by an illumination element arranged below a living cell is incident to a pyramid lens array module located above the living cell, the pyramid lens array module reflects the incident light beam at the same direction angle, and the reflected light beam is diffracted into direct light and diffracted light when passing through the living cell; the phase plate causes the direct light and the diffracted light to generate a phase difference, and the direct light and the diffracted light having the phase difference interfere with each other on the imaging element to generate a phase difference image of the living cells. The volume of the observation device is greatly reduced, especially the reduction in the height direction enables the observation device to be easily placed in an existing culture box, real-time and full-life-cycle observation of cells is realized, meanwhile, the pollution of the cells and the change of a culture environment are avoided, and the observation efficiency is improved. The advantages of a current phase difference type microscopic observation system in the aspect of image effects are reserved, and the requirements for precision and stability of a movement mechanism are lowered.

The invention provides a polypeptide for activating cellular immunity in chronic hepatitis B and application of the polypeptide, and belongs to the technical field of biological medicine. A polypeptide library is designed and synthesized on the basis of a preS1 structural domain for coding HBV large HBsAg, a full-length core protein Core, a polymerase protein fragment rich in T cell epitopes and an mRNA-PreS1CPX holoantigen sequence (as shown in SEQ ID NO.1) of full-length X protein HBX, and peptide fragments capable of activating T cell immunity are screened by utilizing ELISPOT and flow cytometry. Experimental results show that the polypeptide sequences as shown in SEQ ID NO.2-15 can promote HBV antigen specific immune response by stimulating CD8 + T lymphocytes to secrete IFN-gamma, so that immune activation treatment of hepatitis B is realized.