550results about "Mutant preparation" patented technology

The invention discloses bacillus velezensis Scm3 and application thereof.The bacillus velezensis Scm3 is obtained by screening a strain Scm with an inhibition effect on aeromonas hydrophila from turbot intestinal contents and then conducting ultraviolet mutagenesis on the strain to obtain a strain Scm3 with an enhanced antagonism effect on aeromonas hydrophila, and the strain Scm3 is applied to the preparation of the bacillus velezensis Scm3 and the application of the bacillus velezensis Scm3 to prepare the bacillus velezensis Scm3. 16SrDNA (deoxyribonucleic acid) sequence identification and genome sequencing prove that the strain is bacillus velezensis, and the inhibition mechanism of the bacillus velezensis Scm3 on aeromonas hydrophila is researched by a non-contact co-culture method.

Some aspects of this disclosure provide strategies, systems, reagents, methods, and kits that are useful for the targeted editing of nucleic acids, including editing a single site within the genome of a cell or subject, e.g., within the human genome. In some embodiments, fusion proteins of Cas9 and nucleic acid editing enzymes or enzyme domains, e.g., deaminase domains, are provided. In some embodiments, methods for targeted nucleic acid editing are provided. In some embodiments, reagents and kits for the generation of targeted nucleic acid editing proteins, e.g., fusion proteins of Cas9 and nucleic acid editing enzymes or domains, are provided.

The invention discloses propionibacterium capable of obtaining high-yield propionic acid through compound mutation screening and a method for fermenting propionic acid by using the propionibacterium. The high-yield propionic acid strain YCBS010 (CGMCC No.31160) is obtained by carrying out compound mutagenesis on acid-producing propionibacterium CGMCC 1.2232 serving as an original strain through microwave-ultrasonic combined mutagenesis and compound normal-pressure and room-temperature plasma (ARTP) mutagenesis means. According to the invention, YPA15 is used as a starting strain, microwave and ultrasonic combined mutagenesis is carried out for 100 seconds to screen a bacterial strain with increased propionic acid yield, then ARTP mutagenesis is carried out for 90 seconds to screen out a high-yield mutant strain YCBS010, fermentation in a 1-ton tank verifies that the highest propionic acid yield reaches 65.87 g / L and the fermentation intensity is 0.73 g / L.h after fermentation is carried out for 90 hours, and the bacterial strain has better industrial fermentation potential.

The invention discloses an EMS-ARTP compound mutation chlorella pyrenoidosa mutant strain and an application thereof. The preparation method comprises the following steps: mutagenizing a chlorella pyrenoidosa mutant strain CX41 by adopting an ethyl methanesulfonate (EMS) and normal-pressure and room-temperature plasma (ARTP) composite technology, screening and verifying to obtain a golden yellow chlorella pyrenoidosa mutant strain, naming the golden yellow chlorella pyrenoidosa mutant strain as chlorella pyrenoidosa Y11, and screening and verifying the golden yellow chlorella pyrenoidosa Y11 to obtain the chlorella pyrenoidosa Y11. The strain is preserved in Guangdong Microbial Culture Collection Center (GDMCC) on the fifth floor of the building 59, No. 100 courtyard, Xianlie Middle Road, Guangzhou on October 23, 2024, and the preservation number is GDMCC No: 65324. The chlorella pyrenoidosa mutant strain Y11 has the characteristics of high yield of protein and rich carotenoids, and can be used for fermentation production of microalgae protein and carotenoids.

The invention belongs to the technical field of microbial mutagenesis, and particularly relates to a microbial mutagenesis breeding device and application thereof. The microorganism mutation breeding device comprises a cube structure and a transmission structure, the periphery of the cube structure is wrapped with a light-proof material, and an ultraviolet lamp is arranged in the cube structure; the transmission structure comprises a single sequencing area and a mutagenesis area, and the mutagenesis area is inserted into the cube structure through the insertion opening of the cube structure and is perpendicular to the ultraviolet lamp. Microorganisms can be singly sorted through the first horizontal spiral conveying pipeline in the single sorting area and conveyed to the second horizontal spiral conveying pipeline in the mutagenesis area, so that the time and the intensity of ultraviolet irradiation of the microorganisms are uniform, the mutagenesis fatality rate of the microorganisms is remarkably reduced, the forward mutation rate is remarkably increased, and the mutagenesis rate of the microorganisms is remarkably increased. Operation is simple, workload is small, and period is short. In addition, the microorganisms are treated by using a protective agent with specific composition before single sequencing, so that the mutation uniformity and the forward mutation rate can be further improved.

Strategies, systems, methods, reagents, and kits for phage-assisted continuous evolution are provided herein. These include strategies, systems, methods, reagents, and kits allowing for stringency modulation to evolve weakly active or inactive biomolecule variants, negative selection of undesired properties, and / or positive selection of desired properties.

The present disclosure relates to enriched engineered glycoprotein populations with a therapeutically optimized heterogeneous glycan profile. The heterogeneous glycan profile comprises sialylated complex type glycans, which are associated with improved efficacy of the glycoprotein. The present disclosure also relates to compositions and uses of the engineered glycoprotein populations and cell-based method of producing them.

Provided herein are binding molecules comprising a human neonatal Fc receptor (FcRn) binding molecule and a moiety that does not specifically bind to a human antigen. Polynucleotides, vectors, host cells, and methods of production are also provided herein. Methods of treating an antibody-mediated disorder with an FcRn binding molecule or an FcRn / antigen-binding molecule are further provided.

The invention relates to the technical field of microorganisms, in particular to streptomyces noursei and application of the streptomyces noursei in preparation of polymycotin B. The invention provides streptomyces noursei GBC A-20 which is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms, and the preservation number of the streptomyces noursei GBC A-20 is CGMCC No.27277. The strain can be used for efficiently fermenting and producing the multi-fungal element B by utilizing cheap raw materials, the yield of the multi-fungal element B is remarkably improved, and the chemical titer and the biological titer of the fermentation product nysfungin are greatly improved; the strain and the application thereof provide an economical and feasible solution for the industrial production of the multi-fungal element B and the nysfungin.

The invention belongs to the technical field of microorganism mutation breeding, and particularly relates to a nisin high-yield mutant strain with enhanced production performance. The mutant strain is specifically lactococcus lactis 2686, and the preservation number of the mutant strain is CCTCC (China Center for Type Culture Collection) NO: M 2023219. The strain has relatively high osmotic pressure tolerance, can still normally grow and produce nisin when the osmotic pressure is higher than 0.66 osM / kg H2O, meanwhile, the strain has excellent nisin production capacity, the titer reaches 9834 IU / mL when the strain is fermented in a shake flask for 21 hours, and the titer reaches 21000 IU / mL when the strain is fermented in a fermentation tank for 22 hours. The method is high in yield, good in stability, low in production cost and suitable for further industrial application.

The present invention provides a protein variant, a Corynebacterium genus microorganism comprising the protein variant, and a method for producing L-lysine using the microorganism of the present disclosure.

A microorganism according to the present disclosure is a microorganism (accession number: NITE BP-03832) belonging to Bacillus velezensis, a microorganism (accession number: NITE BP-04073) belonging to Bacillus velezensis, a microorganism (accession number: NITE BP-04126) belonging to Bacillus velezensis, or a variant of these having a plant disease prevention effect.

Telomerase reverse transcriptase (TERT) expression enhancing compounds, and methods for using the same, are provided. These compounds and methods find use in a variety of applications in which increased expression of telomerase reverse transcriptase is desired.

A Schizochytrium algae strain rich in n-3 fatty acid and a screening and culture method therefor. Compared with a conventional strain, the content of EPA and the content of n-3 DPA in the provided strain are greatly improved, and the strain has good genetic stability, fast growth rate and good industrialization prospects. Also provided are a culture medium for mutagenesis of Schizochytrium sp., a culture medium for screening Schizochytrium sp., and a suitable mutagenesis method and screening method.

The invention provides a phaffia rhodozyma mutant strain for high yield of astaxanthin as well as a screening method and application of the phaffia rhodozyma mutant strain, belongs to the technical field of fermentation microorganism screening, and can solve the problems of low astaxanthin yield, high cost and low growth temperature of wild type phaffia rhodozyma existing in synthesis of astaxanthin from a phaffia rhodozyma strain. The invention provides a phaffia rhodozyma mutant strain capable of producing astaxanthin at high yield, the phaffia rhodozyma mutant strain is a phaffia rhodozyma AA0529 mutant strain, the phaffia rhodozyma AA0529 mutant strain is preserved in China Center for Type Culture Collection in Wuhan, Hubei on September 7, 2023, and the preservation number is CCTCC (China Center for Type Culture Collection) NO: M20231637. According to the method, the yield of the astaxanthin can be increased, and the yield of the astaxanthin is increased to 3.3 mg / g at 26 DEG C.

A method and application of Escherichia coli BL21star (DE3) BLBRTXST3 and its fermentation method for producing high-purity lactose-N-neotetraose, the invention relates to the technical field of human milk oligosaccharide production, Escherichia coli )BL21 star (DE3) BLBRTXST3 is deposited in the General Microbiology Center of China Microorganism Culture Collection Committee, with the deposit number CGMCC No. 32521 and the deposit date November 6, 2024. The address of the deposit institution is No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing; Escherichia coli ( Escherichia coli )BL21 star (DE3) BLBRTXST3 is used in the fermentation production of lactose-N-neotetraose (LNnT). The present invention improves the yield of lactose-N-neotetraose, uses a chromatographic simulated moving bed to produce high-purity lactose-N-neotetraose with a purity greater than 94%, replaces the traditional solvent crystallization method, has simple separation and purification, is conducive to industrial production, and simultaneously produces linear lactose-N-neohexaose with a purity greater than 95%.

The invention discloses a method for screening high-yield recombinant human lactoferrin through nucleic acid aptamer-assisted mutagenesis of a trichoderma reesei strain and application, and belongs to the technical field of microorganisms. The nucleic acid aptamer Lac-6a-FAM-BHQ1 capable of changing the fluorescence intensity according to the content of lactoferrin is constructed on the basis of the aptamer Lac-6a, and the nucleic acid aptamer can be used for rapidly selecting a mutant strain for producing high-titer recombinant human lactoferrin. The ARTP mutagenesis technology is combined with a nucleic acid aptamer auxiliary screening system, the mutant strain with the improved recombinant human lactoferrin yield is rapidly obtained from a large number of mutant strains, and the yield reaches 274.57 mg / L and is improved by 24.8% compared with that of an original strain. After the genes 111681 and 62716 are over-expressed in T. reesei, compared with a control strain, the yields of the recombinant human lactoferrin of the recombinant strain are respectively increased by 6.9% and 22.1%.

In traditional plant breeding approaches, chemical mutagenesis may be utilized to introduce nucleotide substitutions at random in the genome of a plant, i.e. without possibilities to control the sites of nucleotide changes. Because of genome complexities, the statistical probability is extremely little when it comes to finding a predetermined nucleotide substitution. The present invention, however, demonstrates how a novel, alternative use of digital polymerase chain reaction (dPCR), preferably droplet dPCR (ddPCR), is developed to exploit finding of specific nucleotide substitutions in mutated genes. The entire platform comprises a screening method with a library of mutagenized organisms, digital PCR-based systems and a set-up to propagate and analyze identified, mutated organisms.

The invention relates to the technical field of industrial biology, in particular to streptomyces albus SY6 and a fermentation seed and application thereof, the streptomyces albus SY6 is characterized in that the streptomyces albus SY6 is preserved in China General Microbiological Culture Collection Center on November 13, 2024, the preservation number is CGMCC No.32602, and the streptomyces albus is classified and named as streptomyces albus. The streptomyces albidus SY6 screened by the invention has a better promotion effect in the aspects of mediating a mould signal and strengthening epsilon-polylysine synthesis.

The invention provides a morchella sextelata strain pleurospermum hookeri No.5 and application thereof, and relates to the technical field of strain breeding, the morchella sextelata strain pleurospermum hookeri No.5 is subjected to aerospace carrying to induce genetic variation, then characters capable of being stably inherited are screened out through subculture, and the morchella sextelata strain pleurospermum hookeri No.5 is obtained through fruiting screening test screening. The pleurospermum hookeri

Certain one or more embodiments of the instant disclosure are related to, inter alia, recombinant (genetically modified) filamentous fungal cells (strains) producing proteins of interest, methods and compositions for the design and construction of modified filamentous fungal cells producing proteins of interest, methods and compositions for the expression / production / secretion / recovery and the like of endogenous and / or heterologous proteins of interest (e.g., phytases, lipases, glucoamylases, phospholipases, esterases, cellulases, hemicellulases, xylanases, etc.) in modified filamentous fungal cells, methods and compositions for producing proteins of interest in recombinant filamentous fungal, wherein the proteins produced and secreted into the broth and / or proteins recovered from the broth have uniform and consistent N-linked glycosylation patterns, and / or reduced (unwanted) glycation of one or more proteins of interest, and the like.

The invention discloses a fermentation process for shortening a fermentation period and efficiently synthesizing Ectoine. The fermentation process comprises the following steps: screening and activating high-yield strains, preparing a fermentation culture medium, performing fermentation culture, performing sectional dissolved oxygen regulation and control, performing double-stage pH dynamic regulation and control, performing carbon-nitrogen ratio dynamic regulation based on metabolic flow analysis, performing osmotic pressure gradient induction in the later stage of fermentation, and extracting and purifying a product. According to the fermentation process for shortening the fermentation period and efficiently synthesizing the Ectoin, the expression quantity of key enzymes for synthesizing the Ectoin is increased by 80% or above compared with that of an original strain through the Halomonassp.ECT-09 strain bred through ultraviolet-nitrosoguanidine compound mutation, the basic yield reaches 35 g / L, a foundation is laid for high yield, and the method is suitable for industrial production. A glucose-trehalose mixed carbon source and a yeast extract-ammonium nitrate mixed nitrogen source are adopted, and an L-aspartic acid-L-asparagine mixed precursor is added, so that the metabolic pathway is shortened, and the loss of an intermediate product is reduced.

The invention provides Saccharomyces cerevisiae for producing squalene as well as a screening method and application of the Saccharomyces cerevisiae. The method comprises the following steps: culturing a Saccharomyces cerevisiae engineering strain ySC782 for synthesizing squalene to obtain a to-be-mutagenized bacterial solution, carrying out ARTP mutagenesis on the to-be-mutagenized bacterial solution, adding the to-be-mutagenized bacterial solution into a YPD liquid culture medium, culturing to obtain a resuscitation bacterial solution, adding the resuscitation bacterial solution into a sucrose solution with gradient concentration distribution, screening, and repeating the ARTP mutagenesis-sucrose solution screening step to obtain the squalene mutant strain. And finally, the saccharomyces cerevisiae strain ySC782-M5 is obtained through screening, and the saccharomyces cerevisiae strain ySC782-M5 is obtained through screening. The yield of squalene produced by the saccharomyces cerevisiae ySC782-M5 reaches 49.83 g / L, so that a solution is provided for breaking through the yield bottleneck of squalene synthesized by yeast.

The invention discloses a method for screening and cultivating a bolete mutant strain, which belongs to the field of microorganisms, and mainly comprises the following steps: obtaining the bolete mutant strain by using a carbon ion beam irradiation technology, naming the bolete mutant strain as ng2, and preserving the bolete mutant strain with the preservation number of CCTCC (China Center for Type Culture Collection) M 2025077; inoculating the ng2 strain into a hypha rapid germination and propagation solid culture medium PDA or PDA + peptone + beef extract + yeast extract powder, wherein hypha begins to germinate after 4 days; the method comprises the following steps: inoculating a solid strain into a hypha rapid propagation liquid culture medium including cane sugar, corn flour, soybean flour, wheat bran, monopotassium phosphate and yeast extract powder, and generating a large amount of fungus balls within 7 days; inoculating a liquid strain into a fruiting culture raw material self-made fermentation material, corn straw, turfy soil and rice, wherein hyphae overgrow in 30 days; the ng2 strain is high in activity, high in growth speed and suitable for being used as an excellent bolete strain in the Heilongjiang region; the screening and cultivating method of the bolete mutant strain is mainly used for solving the problems that the propagation efficiency of the bolete strain is low, germplasm resources in northern areas such as Heilongjiang are deficient and the like.

The invention provides a Morchella septimelata strain pleurospermum hookeri No.18 and application thereof, and relates to the technical field of strain breeding, the Morchella septimelata strain pleurospermum hookeri No.18 is obtained by space-carrying induced genetic variation, then subculture is carried out to screen out stably inherited characters, and the Morchella septimelata strain pleurospermum hookeri No.18 is screened through a fruiting screening test. According to the method, the hypha adaptability, resistance, harvesting concentration and commercial mushroom quality are remarkably improved, the method can be widely applied to cultivation and production of morchella septimelata, the acre yield of morchella septimelata can be greatly increased, and the method has a wide application prospect.

To provide methods for mutating wild type Saccharomyces cerevisiae yeast to enhance the ability to produce both glutathione and aldehyde dehydrogenase.SOLUTION: The invention relates to a yeast strain that produces glutathione (GSH) and an aldehyde dehydrogenase (acetaldehyde dehydrogenase). More specifically, the invention relates to Saccharomyces cerevisiae Kwon P-1 KCTC13925BP, Saccharomyces cerevisiae Kwon P-2 KCTC14122BP and Saccharomyces cerevisiae Kwon P-3 KCTC14123BP that produce both glutathione and an aldehyde dehydrogenase.SELECTED DRAWING: Figure 6

The invention belongs to the technical field of microorganisms, and particularly relates to Chryseobacterium sp. 2R14A and application of the Chryseobacterium sp. 2R14A. The strain is excellent in oxidation resistance, still can grow after being treated by 80mM hydrogen peroxide for 30 minutes, and is not completely inactivated after being treated by 160mM hydrogen peroxide, and a genome contains antioxidant related genes; growth conditions are mild, sodium chloride is not needed, large-scale culture is easy, cost is low, and physiological and biochemical characteristics are stable. The strain can be used for treating oxidizing agent-containing polluted environment and improving stress resistance of crops or microorganisms, can also be used as a gene engineering recipient bacterium or a mutation breeding material, and expands application in the fields of agriculture, environment and medicine.

The present invention discloses a preparation method of a filtrate of fermentation products of Bifidobacterium longum and its lysate, which relates to the technical field of microorganisms. A strain of Bifidobacterium longum CGMCC No. 31133 with fast growth rate, high cell density, rich and high-content metabolites is obtained through strain mutagenesis and breeding. The strain is used for fermenting to prepare a filtrate of fermentation products and a lysate. The filtrate is rich in acetic acid, lactic acid, amino acids, proteins and Bifidobacterium longum polysaccharides, and has remarkable skin brightening and moisturizing effects. The alkali lysis method is used for cell wall breaking, which not only makes the release of intracellular active substances more complete, but also effectively utilizes the active components of cell walls and cell membranes. The prepared lysate has richer active components and is easy to absorb, further enhancing the antioxidant, anti-aging, skin brightening and repair performance of the lysate. The filtrate of fermentation products and the lysate prepared by the present invention not only have rich active components and excellent performance, but also have a simple process and a short production cycle, and have significant cost advantages in production.

The invention provides a microorganism as well as an application and a method of the microorganism in preparation of 1, 3-propylene glycol and lactic acid, the microorganism is lactobacillus reuteri, the microorganism is preserved in Guangdong Microbial Culture Collection Center on May 29, 2024, and the preservation number is GDMCC NO: 64700. The invention further provides a method for preparing 1, 3-propylene glycol and lactic acid, and the microorganism is lactobacillus reuteri and is preserved in Guangdong Microbial Culture Collection Center on May 29, 2024. The Lactobacillus reuteri can co-produce 1, 3-propylene glycol and lactic acid, has the advantages of high yield of 1, 3-propylene glycol and lactic acid, fast growth and metabolism, strong stability, high safety and the like, provides a new idea for green, low-cost and efficient production of 1, 3-propylene glycol and lactic acid, and has a wide application prospect.

The invention relates to an L-alanine high-yield strain, an extraction method and extraction equipment, and relates to the technical field of L-alanine high-yield strains, the L-alanine high-yield strain comprises a standing extraction platform, an extraction support, an extraction guide rail and a strain extractor, the strain extractor comprises a plurality of adjacently distributed extraction filter plates, and a working inner cavity is formed in each extraction filter plate; a microwave generator is arranged in the working inner cavity of the extraction filter plate, and working outer cavities are formed in the two sides of the extraction filter plate and used for crystallizing the mixed initial strains; according to the method, the mixed solution is crystallized, so that the solution is changed from a liquid state to a solid state, at the moment, the solution can be screened, impurities and metabolites in a crystalline product are removed, and at the moment, the L-alanine high-producing strain is extracted, so that the influence of the impurities on the extraction of the L-alanine high-producing strain can be greatly avoided; the extraction efficiency of the L-alanine high-yield strain is ensured.