275results about "Mutant preparation" patented technology

The present disclosure relates to enriched engineered glycoprotein populations with a therapeutically optimized heterogeneous glycan profile. The heterogeneous glycan profile comprises sialylated complex type glycans, which are associated with improved efficacy of the glycoprotein. The present disclosure also relates to compositions and uses of the engineered glycoprotein populations and cell-based method of producing them.

The invention provides a phaffia rhodozyma mutant strain for high yield of astaxanthin as well as a screening method and application of the phaffia rhodozyma mutant strain, belongs to the technical field of fermentation microorganism screening, and can solve the problems of low astaxanthin yield, high cost and low growth temperature of wild type phaffia rhodozyma existing in synthesis of astaxanthin from a phaffia rhodozyma strain. The invention provides a phaffia rhodozyma mutant strain capable of producing astaxanthin at high yield, the phaffia rhodozyma mutant strain is a phaffia rhodozyma AA0529 mutant strain, the phaffia rhodozyma AA0529 mutant strain is preserved in China Center for Type Culture Collection in Wuhan, Hubei on September 7, 2023, and the preservation number is CCTCC (China Center for Type Culture Collection) NO: M20231637. According to the method, the yield of the astaxanthin can be increased, and the yield of the astaxanthin is increased to 3.3 mg / g at 26 DEG C.

The invention discloses a method for screening high-yield recombinant human lactoferrin through nucleic acid aptamer-assisted mutagenesis of a trichoderma reesei strain and application, and belongs to the technical field of microorganisms. The nucleic acid aptamer Lac-6a-FAM-BHQ1 capable of changing the fluorescence intensity according to the content of lactoferrin is constructed on the basis of the aptamer Lac-6a, and the nucleic acid aptamer can be used for rapidly selecting a mutant strain for producing high-titer recombinant human lactoferrin. The ARTP mutagenesis technology is combined with a nucleic acid aptamer auxiliary screening system, the mutant strain with the improved recombinant human lactoferrin yield is rapidly obtained from a large number of mutant strains, and the yield reaches 274.57 mg / L and is improved by 24.8% compared with that of an original strain. After the genes 111681 and 62716 are over-expressed in T. reesei, compared with a control strain, the yields of the recombinant human lactoferrin of the recombinant strain are respectively increased by 6.9% and 22.1%.

Certain one or more embodiments of the instant disclosure are related to, inter alia, recombinant (genetically modified) filamentous fungal cells (strains) producing proteins of interest, methods and compositions for the design and construction of modified filamentous fungal cells producing proteins of interest, methods and compositions for the expression / production / secretion / recovery and the like of endogenous and / or heterologous proteins of interest (e.g., phytases, lipases, glucoamylases, phospholipases, esterases, cellulases, hemicellulases, xylanases, etc.) in modified filamentous fungal cells, methods and compositions for producing proteins of interest in recombinant filamentous fungal, wherein the proteins produced and secreted into the broth and / or proteins recovered from the broth have uniform and consistent N-linked glycosylation patterns, and / or reduced (unwanted) glycation of one or more proteins of interest, and the like.

The invention discloses a fermentation process for shortening a fermentation period and efficiently synthesizing Ectoine. The fermentation process comprises the following steps: screening and activating high-yield strains, preparing a fermentation culture medium, performing fermentation culture, performing sectional dissolved oxygen regulation and control, performing double-stage pH dynamic regulation and control, performing carbon-nitrogen ratio dynamic regulation based on metabolic flow analysis, performing osmotic pressure gradient induction in the later stage of fermentation, and extracting and purifying a product. According to the fermentation process for shortening the fermentation period and efficiently synthesizing the Ectoin, the expression quantity of key enzymes for synthesizing the Ectoin is increased by 80% or above compared with that of an original strain through the Halomonassp.ECT-09 strain bred through ultraviolet-nitrosoguanidine compound mutation, the basic yield reaches 35 g / L, a foundation is laid for high yield, and the method is suitable for industrial production. A glucose-trehalose mixed carbon source and a yeast extract-ammonium nitrate mixed nitrogen source are adopted, and an L-aspartic acid-L-asparagine mixed precursor is added, so that the metabolic pathway is shortened, and the loss of an intermediate product is reduced.

The invention provides Saccharomyces cerevisiae for producing squalene as well as a screening method and application of the Saccharomyces cerevisiae. The method comprises the following steps: culturing a Saccharomyces cerevisiae engineering strain ySC782 for synthesizing squalene to obtain a to-be-mutagenized bacterial solution, carrying out ARTP mutagenesis on the to-be-mutagenized bacterial solution, adding the to-be-mutagenized bacterial solution into a YPD liquid culture medium, culturing to obtain a resuscitation bacterial solution, adding the resuscitation bacterial solution into a sucrose solution with gradient concentration distribution, screening, and repeating the ARTP mutagenesis-sucrose solution screening step to obtain the squalene mutant strain. And finally, the saccharomyces cerevisiae strain ySC782-M5 is obtained through screening, and the saccharomyces cerevisiae strain ySC782-M5 is obtained through screening. The yield of squalene produced by the saccharomyces cerevisiae ySC782-M5 reaches 49.83 g / L, so that a solution is provided for breaking through the yield bottleneck of squalene synthesized by yeast.

To provide methods for mutating wild type Saccharomyces cerevisiae yeast to enhance the ability to produce both glutathione and aldehyde dehydrogenase.SOLUTION: The invention relates to a yeast strain that produces glutathione (GSH) and an aldehyde dehydrogenase (acetaldehyde dehydrogenase). More specifically, the invention relates to Saccharomyces cerevisiae Kwon P-1 KCTC13925BP, Saccharomyces cerevisiae Kwon P-2 KCTC14122BP and Saccharomyces cerevisiae Kwon P-3 KCTC14123BP that produce both glutathione and an aldehyde dehydrogenase.SELECTED DRAWING: Figure 6

The invention belongs to the technical field of microorganisms, and particularly relates to Chryseobacterium sp. 2R14A and application of the Chryseobacterium sp. 2R14A. The strain is excellent in oxidation resistance, still can grow after being treated by 80mM hydrogen peroxide for 30 minutes, and is not completely inactivated after being treated by 160mM hydrogen peroxide, and a genome contains antioxidant related genes; growth conditions are mild, sodium chloride is not needed, large-scale culture is easy, cost is low, and physiological and biochemical characteristics are stable. The strain can be used for treating oxidizing agent-containing polluted environment and improving stress resistance of crops or microorganisms, can also be used as a gene engineering recipient bacterium or a mutation breeding material, and expands application in the fields of agriculture, environment and medicine.

The invention provides a microorganism as well as an application and a method of the microorganism in preparation of 1, 3-propylene glycol and lactic acid, the microorganism is lactobacillus reuteri, the microorganism is preserved in Guangdong Microbial Culture Collection Center on May 29, 2024, and the preservation number is GDMCC NO: 64700. The invention further provides a method for preparing 1, 3-propylene glycol and lactic acid, and the microorganism is lactobacillus reuteri and is preserved in Guangdong Microbial Culture Collection Center on May 29, 2024. The Lactobacillus reuteri can co-produce 1, 3-propylene glycol and lactic acid, has the advantages of high yield of 1, 3-propylene glycol and lactic acid, fast growth and metabolism, strong stability, high safety and the like, provides a new idea for green, low-cost and efficient production of 1, 3-propylene glycol and lactic acid, and has a wide application prospect.

The invention belongs to the field of microbial breeding and fermentation engineering, and particularly relates to a saccharomyces cerevisiae mutagenesis strain with high yield of S-adenosyl-L-methionine as well as a mutagenesis method and application of the saccharomyces cerevisiae mutagenesis strain. Saccharomyces cerevisiae is induced to generate genetic diversity through multiple rounds of ultraviolet mutagenesis, and high-throughput screening is performed in combination with strain color phenotypic difference; and the mutant strain HY2402-Z18 with high yield of S-adenosine-L-methionine and genetic stability is obtained. The SAM yield of the mutagenic strain is increased by 40.1% compared with that of an original strain, the SAM yield reaches 13.44 g / L when the mutagenic strain is fermented for 60 h in a 5 L fermentation tank system, the unit yield reaches 0.156 g / L / OD, and the methionine conversion rate is 75%. After thalli are collected through centrifugation of fermentation liquor, an S-adenosine-L-methionine product with the purity of 98%-100% can be obtained through purification of ion exchange resin, no by-products are accumulated, the yield, the substrate conversion rate and the production efficiency of the S-adenosine-L-methionine can be remarkably improved when the mutagenesis strain is used for fermentation production, and the production cost is reduced. The production cost is effectively reduced, the product purity and quality are improved, and the method is suitable for industrial large-scale production of SAM.

The application discloses a Yarrowia lipolytica strain with high yield of gamma-decalactone and / or delta-decalactone and application thereof, and belongs to the technical field of biotechnology. The name of the strain is Yarrowia lipolytica Y-1, and the preservation number is GDMCC No: 67810. The strain Y-1 can be directly used for biological transformation to cooperatively and efficiently prepare gamma-decalactone and delta-decalactone with castor oil acid as a substrate. The strain has three outstanding advantages: ① yield advantage; ② tolerance advantage; and ③ application advantage. The biological transformation method has the advantages of mild reaction condition, low cost, product meeting natural perfume standards, environmental friendliness and the like.

The invention relates to a gene expression cassette and application thereof. The gene expression cassette comprises a tyrosinase gene, a transcriptional repressor protein gene, a melanin response promoter and a reporter gene, and the reporter gene is located at the downstream of the melanin response promoter and is regulated by the promoter. The gene expression cassette with a specific structure is designed, melanin in cells can be responded, a melanin biosensor can be further constructed by utilizing the gene expression cassette, the gene expression cassette has good specificity, and high-throughput screening of strains can be realized. Furthermore, ALE evolution is carried out on the strain for producing melanin, high-throughput screening is carried out based on a designed sensor, the strain for producing melanin with high yield is obtained, through shake-flask culture and 5L fermentation tank culture, the yield of melanin reaches up to 10.84 g / L and 29.04 g / L respectively, and an efficient and feasible solution is provided for large-scale production of melanin.

This invention relates to a hangover relief composition containing a dried powder, lysate, or extract of enzymes that produce glutathione and aldehyde dehydrogenase. Specifically, this invention relates to a hangover relief composition containing dried powder, lysate, or extract of *Saccharomyces cerevisiae* Kwon P-1 KCTC 13925BP, *Saccharomyces cerevisiae* Kwon P-2 KCTC14122BP, and *Saccharomyces cerevisiae* Kwon P-3 KCTC14123BP, which simultaneously produce glutathione and aldehyde dehydrogenase.

The present invention relates to yeast strains of the species Saccharomyces pastorianus that have the useful property of producing low levels of diacetyl during fermentation. Methods for producing malt- and / or grain-based beverages using these strains, as well as the beverages produced thereby, are also provided.

A method for producing a fermented milk product (e.g. a yogurt) with a relatively high stable pH value at the end of the fermentation comprising inoculating milk with Streptococcus thermophilus (ST) Gal(+) bacteria.

The invention discloses chlorella capable of resisting acetate and urea and a breeding method and application thereof, and relates to the technical field of microorganisms, the chlorella is a Sorokiniana mutant strain, the classification name of the chlorella is Chlorella sorokiniana-TongWei-01, the chlorella is preserved in the China General Microbiological Culture Collection Center at 2025-12-1, and the preservation number of the chlorella is CGMCC NO. The address of the preservation unit is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.46630. According to the method, large-scale culture can be performed by taking acetic acid / sodium acetate as a unique carbon source and urea as a unique nitrogen source, the method has excellent acetic acid utilization efficiency and urea tolerance, toxicity interference is resisted while two substrates are efficiently converted, high-density culture supported by a cheap carbon-nitrogen source is realized, and a core algae species support is provided for large-scale production of microalgae.

Some aspects of this disclosure provide compositions, methods, systems, and kits for controlling the activity and / or improving the specificity of RNA-programmable endonucleases, such as Cas9. For example, provided are guide RNAs (gRNAs) that are engineered to exist in an “on” or “off” state, which control the binding and hence cleavage activity of RNA-programmable endonucleases. Some aspects of this disclosure provide mRNA-sensing gRNAs that modulate the activity of RNA-programmable endonucleases based on the presence or absence of a target mRNA. Some aspects of this disclosure provide gRNAs that modulate the activity of an RNA-programmable endonuclease based on the presence or absence of an extended DNA (xDNA).

The invention relates to a high-yield protein glutaminase Chryseobacterium prion strain and a preparation method thereof. The strain is obtained by carrying out ARTP and NTG compound mutagenesis treatment on an original strain of Chryseobacterium prion and carrying out high-throughput screening on the strain subjected to the compound mutagenesis treatment. The strain is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC M 20251792. The invention also relates to a method for preparing protein glutaminase by using the strain of the invention. The enzyme activity of the protein glutaminase of the strain is 6 times that of an original strain, and the production efficiency of the protein glutaminase can be remarkably improved.

The invention relates to the technical field of microbial fermentation and food processing, and particularly discloses Weissella fusiformis JMF-006 as well as a mutagenesis method and application thereof. The invention provides a Weissella fusiformis JMF-006 with the exopolysaccharide yield of 20-25g / L. The Weissella fusiformis JMF-006 is obtained by breeding through a mutation breeding method of a high-yield EPS strain, and the mutation breeding method can also be used for culturing other high-yield EPS strains. The invention provides a strain composition, the strain composition comprises Weissella fusiformis JMF-006 or a high-yield EPS strain, other lactic acid bacteria strains (such as lactobacillus plantarum JMF-001 and lactobacillus casei JMF-002) can be further compounded, and the obtained strain composition has more excellent fermentation performance. When the Weissella fusiformis JMF-006, the high-yield EPS strain and the strain composition provided by the invention are applied to the fermentation process of food-derived drinks, a fermented product with stable properties and better flavor can be obtained, and the technical problems that the yield of extracellular polymeric substances is low, the fermentation performance is poor and the application range is narrow in an existing lactic acid bacteria starter are solved.

The invention discloses a method for preventing and treating sclerotinia rot of colza by antagonistic trichoderma strains, and relates to the technical field of agricultural disease prevention and control, and the method comprises the following specific steps: S100, strain screening and mutagenesis: separating trichoderma strains from rhizosphere soil of colza in different environments, primarily screening strains with antagonistic action on sclerotinia sclerotiorum by a plate confrontation method, and carrying out mutagenesis on the strains with antagonistic action on sclerotinia sclerotiorum; carrying out chemical mutagenesis treatment on the primarily screened strains, carrying out confrontation culture on the primarily screened strains and sclerotinia sclerotiorum again, screening out high-yield antagonistic substances with larger inhibition zones and mutant strains with strong stress resistance, and culturing to obtain trichoderma fermentation liquor; by obtaining the trichoderma strains which can keep efficient antagonism and strong stress resistance under different environmental conditions, the stability of the trichoderma strains on disease control is enhanced, so that the rape can effectively resist invasion of sclerotiniose under different environmental conditions in the growth process, and the disease resistance of the rape is improved. And antagonistic active substances are extracted to prepare a biological fertilizer carrier, and the biological fertilizer carrier is applied to the planting process of oilseed rape, so that the growth and reproduction of sclerotinia sclerotiorum are effectively inhibited.

The invention discloses a breeding method of lactic acid bacteria with high phenyllactic acid yield, and relates to the technical field of lactobacillus breeding. The invention provides a method for breeding lactic acid bacteria by using a normal-pressure room-temperature plasma (ARTP) mutagenesis technology, which can be used for simply, quickly and effectively screening lactic acid bacteria strains with high yield of phenyllactic acid, and has industrial application value.

The present disclosure relates to: a protein variant; a polynucleotide encoding the protein variant; a Corynebacterium genus microorganism comprising at least one selected from the group consisting of the protein variant and the polynucleotide; and a method for producing L-isoleucine using the microorganism.

The invention provides mutagenic chlorella capable of realizing high yield of phytoene and application of the mutagenic chlorella, and belongs to the technical field of chlorella. The mutagenic algal strain SZU-C49 of the mutagenic chlorella pyrenoidosa is preserved in the Guangdong Microbial Culture Collection Center, the preservation number is GDMCC No: 66780, the preservation time is July 30, 2025, and the preservation address is the 5th floor, No. 59 building, No. 100 Courtyard, Xianlie Middle Road, Guangzhou. The algal strain is obtained by carrying out chemical mutagenesis and screening on a mutagenic chlorella wild type common chlorella original strain. The mutagenic chlorella disclosed by the invention is cultured for 72 hours under an optimized culture condition, the content of phytoene in cells of the strain reaches 25799 mg / g (dry weight) and is more than 100 times that of an original strain, and the mutagenic chlorella can be widely applied to the fields of functional foods, health-care products, cosmetics, medicines and the like.

The invention relates to the technical field of microbial metallurgy, in particular to a method for strengthening leaching of rare earth elements by rare earth-resistant biological acid flora through gradient stress domestication, which comprises the following steps: selecting an original strain of thiobacillus thiooxidans for ultraviolet radiation mutagenesis, and primarily screening mutant strains through a high-throughput screening condition; carrying out compound induction on the culture medium and carrying out four-stage stress domestication on the screened strains; selecting a composite carrier, and curing the domesticated bacterial liquid and the carrier; the industrial microbial inoculum is prepared based on microbial inoculum production and rare earth leaching processes. According to the method for strengthening leaching of the rare earth elements from the rare earth-resistant biological acid flora through gradient stress domestication, rare earth concentration gradient-pH gradient-temperature fluctuation-tailing matrix substitution is constructed as a gradient stress domestication stage, and a composite induction culture medium is combined to realize directed evolution of flora genomes, so that comprehensive improvement of stress resistance indexes is ensured; by designing a composite carrier structure and introducing an immobilization process, a carrier immobilization technology is realized to guarantee industrial stability.

The present invention relates to the specific use of lactose-positive Streptococcus thermophilus strains in cheese production. The strains used according to the invention can carry one or more mutations in the glcK gene encoding glucokinase (which mutations result in reduced glucokinase activity), a mutation in the ccpA gene, and / or a mutation in at least one gene encoding a protein of the mannose-glucose-specific PTS. The invention further relates to a composition comprising the strains used according to the invention in combination with at least one strain of the genus Lactobacillus and / or a strain of mesophilic lactic acid bacteria, a method for producing cheese, and the cheese obtained by this method.

Disclosed herein are yeast strains and derivatives thereof, as well as compositions comprising the yeast strains for use in ethanol production. The disclosure also relates to processes for producing ethanol from biomass using the yeast strains and compositions. In particular, the yeast strains produce less glycerol and more ethanol, and have higher temperature tolerance and faster fermentation rates than strains and products currently used in ethanol production processes.

To provide methods for increasing specificity of RNA-guided genome editing.SOLUTION: The present invention provides a method of increasing specificity of RNA-guided genome editing in a cell, the method comprising contacting the cell with a guide RNA that includes a complementarity region consisting of 17-18 nucleotides that are complementary to 17-18 consecutive nucleotides of the complementary strand of a selected target genomic sequence.SELECTED DRAWING: Figure 2-6

The present disclosure provides compositions and methods directed to anti-CD99 CAR-T therapies for treating cancer, such as DIPG, Ewing Sarcoma, and AML.

The invention relates to a method for improving the gamma-aminobutyric acid yield of heterologous expression coffee GAD recombinant escherichia coli through ultrasonic and ultraviolet combined mutagenesis, and belongs to the technical field of microbial breeding. The method comprises the following steps: mutagenizing recombinant escherichia coli for heterologous expression of coffee-derived GAD by adopting an ultrasonic and ultraviolet sequential compound mutagenesis strategy, screening out bacterial colonies with good growth vigor through mutagenesis, finally inoculating the screened bacterial colonies into a fermentation culture medium for fermentation, and screening out bacterial strains with the highest GABA yield and conversion rate; the GABA yield of the mutagenized strain is improved by 31.3% compared with that of an initial strain, and the conversion rate is improved by 22.1%. The GABA yield, the GAD activity, the GAD gene expression quantity and the passage stability of the high-yield mutant strain UT-UV-3 are tested, the GABA yield, the conversion rate, the GAD activity and the GAD gene expression quantity of the high-yield mutant strain UT-UV-3 are remarkably improved, passage is stable, and the high-yield mutant strain UT-UV-3 has a good application prospect.

The present invention relates to novel strains of Lactobacillus genus having extended shelf life properties, i.e., capable of extending the shelf life of food products (e.g., fermented milk products) at room temperature without severely impairing the antimicrobial, antifungal and / or anti-yeast effect ability of said strains when added to such products. According to another aspect, the present invention relates to an antimicrobial, antifungal or anti-yeast composition. According to yet another aspect, the present invention relates to a food product. According to another aspect, the present invention relates to a method for manufacturing a food product. According to another aspect, the present invention relates to the use of Lactobacillus strains for providing antifungal, antimicrobial and / or anti-yeast effects, for example in fermented milk products.