47results about "Mutant preparation" patented technology

The application discloses a Yarrowia lipolytica strain with high yield of gamma-decalactone and / or delta-decalactone and application thereof, and belongs to the technical field of biotechnology. The name of the strain is Yarrowia lipolytica Y-1, and the preservation number is GDMCC No: 67810. The strain Y-1 can be directly used for biological transformation to cooperatively and efficiently prepare gamma-decalactone and delta-decalactone with castor oil acid as a substrate. The strain has three outstanding advantages: ① yield advantage; ② tolerance advantage; and ③ application advantage. The biological transformation method has the advantages of mild reaction condition, low cost, product meeting natural perfume standards, environmental friendliness and the like.

The present invention relates to yeast strains of the species Saccharomyces pastorianus that have the useful property of producing low levels of diacetyl during fermentation. Methods for producing malt- and / or grain-based beverages using these strains, as well as the beverages produced thereby, are also provided.

A method for producing a fermented milk product (e.g. a yogurt) with a relatively high stable pH value at the end of the fermentation comprising inoculating milk with Streptococcus thermophilus (ST) Gal(+) bacteria.

A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner.

Disclosed are methods of treating or reducing the occurrence of a steatohepatitis disorder. The disorder may include, for example, NASH, parenteral nutrition associated liver disease (PNALD), or genetic forms of liver disease. The method may comprise the step of administering a composition comprising obeticholic acid to an individual in need thereof.

This invention belongs to the field of microbial technology and provides a space-mutated *Bacillus paralichrysiformis* strain with high synthetic selenocysteine ​​and high sodium selenite tolerance, obtained through space mutagenesis, and its applications. The *Bacillus paralichrysiformis* G1-m strain is deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 36525, deposited on November 11, 2025. This strain has significant core advantages: firstly, it possesses extremely high sodium selenite tolerance, able to withstand sodium selenite stress up to 440 mM; secondly, its selenocysteine ​​and nano-selenium synthesis efficiency is significantly improved, not only significantly increasing the strain's resistance threshold to selenium stress but also directionally enhancing the activity of the selenocysteine ​​biosynthetic pathway. Its application potential covers multiple fields, such as selenium-enriched probiotic preparations, nano-drug carriers, functional food additives, and high-efficiency selenium nutritional supplements, laying the foundation for the high-value utilization of selenium resources and the industrial application of functional microorganisms.

The application discloses a method for cloning an avirulence gene from a small chromosome of Magnaporthe grisea, which comprises the following steps: (1) screening a strain carrying an avirulence gene corresponding to an anti-rice blast gene X; (2) performing ultraviolet mutagenesis on the screened strain; (3) spraying the strain after ultraviolet mutagenesis on a rice variety carrying the anti-rice blast gene X, and collecting the avirulence gene mutant strain; (4) performing mixed pool sequencing on the avirulence gene mutant strain obtained in the step (3); and (5) locating the avirulence gene through association analysis. The application does not depend on the fertility of the strain, sexual recombination and the genomic sequence polymorphism among different strains, and the target gene can be located through the association analysis by obtaining the avirulence gene mutant.

The present disclosure relates to: a variant of a protein; a polynucleotide encoding the variant of a protein; a microorganism of the genus Corynebacterium, comprising at least one selected from the group consisting of the variant of a protein and the polynucleotide; and a method for producing L-isoleucine by using the microorganism.

The present disclosure relates to a novel microorganism, a lysate thereof, a culture broth thereof, and anti-allergy uses thereof, wherein a novel strain according to one aspect, a lysate derived from the strain, a culture broth of the strain, an extract of the culture broth, or a mixture thereof may be advantageously used in prevention, alleviation, or treatment of allergic diseases.

Provided are a strain for fermentation production of gentamicin B and use thereof. A high-yield gentamicin B-producing strain, Micromonospora echinospora HS-GT101-23, is obtained through screening by means of atmospheric pressure and room temperature plasma mutagenesis technology. The fermentation process for the strain is simple and can achieve high-yield fermentation production of gentamicin B without the need for feeding. In a shake flask experiment, the titer of gentamicin B produced by the fermentation of the strain reaches 4126 μg / mL; in a fermentation tank, the titer can also reach 3881 μg / mL. Therefore, the strain is suitable for industrial fermentation production. The strain HS-GT101-23 obtained through screening has good stability, and the titer of gentamicin B produced by the strain over 5 consecutive passages remains basically stable at a consistently high level, which lays a foundation for industrial production.

This invention belongs to the field of genetic engineering technology, specifically relating to a mutant strain of Bacillus subtilis and its application in the production of alkaline protease. The applicant will utilize a strain derived from alkaline Bacillus (… Alkalihalobacillus xiaoxiensis The alkaline protease gene of Bacillus subtilis (Bacillus subtilis) Bacillus subtilis Overexpression in the host cell yielded a recombinant integrated expression strain. Further, by knocking out the antibiotic resistance marker gene in the alkaline protease expression, a recombinant strain without the antibiotic selection marker was obtained. This strain was then used as the starting material for UV mutagenesis, and a mutant strain exhibiting significantly increased alkaline protease expression was obtained. This mutant strain was deposited on December 5, 2022, at the China Center for Type Culture Collection (CCTCC), Wuhan University, China, with accession number CCTCC NO: M20221874. This mutant strain can be widely used in the production of alkaline protease, which is beneficial for reducing the production cost of this enzyme.

Methods and compositions comprising carotenoids for the treatment or prevention of nausea, e.g., chemotherapy-induced nausea and vomiting.

The invention relates to a fungal enzymatic composition for degrading plastic products and plastic polymers obtained from the simultaneous cultivation of at least three different species of Trichoderma fungi. Moreover, the present invention also relates to a method for the depolymerization and degradation of plastic-containing waste by the fungal enzymatic composition of the invention.

To provide a method for inducing mutations in algae and a method for evaluating neutron irradiation conditions.SOLUTION: A method for inducing mutations in algae includes irradiating the algae present in water with neutron beams, where the absorbed dose of the neutron beams by the algae is within the range of 0.1 to 100 Gy.SELECTED DRAWING: Figure 1

Guide RNA can be used with an Argonaute (AGO) molecule, wherein said AGO molecule, when loaded with said guide RNA, cleaves a target nucleic acid. This guide RNA can be a cityRNA. Described herein are cityRNAs which have been modified with a Booster nucleic acid to increase their ability to cleave target nucleic acid through AGO.

The present application relates to a kind of high-yield abamectin B2 Streptomyces avermitilis AVE-16 and its preparation method, the Streptomyces avermitilis AVE-16 is preserved in China Microbial Culture Collection Center, and the preservation number is CGMCC No.38200.The present application uses Streptomyces avermitilis XB-12 as starting strain, uses ethyl methanesulfonate and atmospheric pressure plasma combined mutagenesis technology to obtain a Streptomyces avermitilis AVE-16, and the yield of abamectin B2a is increased by 145% compared with starting strain, and the total yield of abamectin (B1a+B2a) is increased by 44% compared with starting strain.

The present application relates to the field of microbial technology, and discloses a rapid screening method for Chlorella mutagenesis strains. The method comprises: (1) mutagenesis treatment of Chlorella algae seed to obtain mutagenesis algae group; (2) at least three rounds of progressive screening of the mutagenesis algae group to obtain high-performance Chlorella; wherein the first round of screening is carried out in a microplate, growth rate is used as the screening index, and the algae strains with growth rate ranking from the top 10% to the top 20% are selected; the second round of screening is carried out in a deep well plate, growth rate is used as the screening index, and the algae strains with growth rate ranking from the top 10% to the top 20% are selected; the third round of screening is carried out in a conical flask, growth rate, biomass and protein content are used as the comprehensive screening index, and high-performance Chlorella is obtained. The high-throughput screening method provided by the present application has the advantages of high efficiency, universality and reliability, can significantly shorten the breeding cycle, and can stably screen high-performance Chlorella strains from different mutagenesis populations.

The present invention relates to a method for cultivating a microorganism, preferably a Saccharomycetales yeast, capable of utilizing ethanol as feedstock. The invention is directed to a method for the production of biomass with increased protein content, in particular single cell protein, wherein the yeast single cell protein product comprises Saccharomycetales yeast cells, as well as to an animal feed comprising such biomass.

This invention discloses a lightweight foamed sand based on a low-temperature resistant microbial solidifying agent and its preparation method. The lightweight foamed sand comprises solid and liquid substances in a mass ratio of 3.5-6:1. The solid substance comprises 78-84 wt% sand, 12-18 wt% composite activator, and 4-6 wt% bacterial foam adsorbent. The liquid substance is a cementing solution. The preparation method includes the following steps: preparing a low-temperature resistant microbial solidifying agent; mixing the prepared low-temperature resistant microbial solidifying agent with sand to obtain a solid substance; and mixing the obtained solid substance with a cementing solution to obtain the lightweight foamed sand based on the low-temperature resistant microbial solidifying agent. The technical solution of this invention significantly improves the survival rate and mineralization efficiency of the microorganisms under low-temperature conditions. The lightweight foamed sand prepared using this technical solution is lighter in weight for the same volume and has higher compressive strength and impermeability.

The present application relates to the field of biotechnology, in particular to a kind of production DHA and astaxanthin schizochytrium strain and its preparation method and application.The present application uses "multi-stage precision metabolic engineering+directional environmental domestication" collaborative modification paradigm, realizes the revolutionary breakthrough of astaxanthin yield, the present application discards traditional random mutagenesis and inefficient screening path, innovatively constructs "precursor reinforcement→intermediate supplement→end product rate-limiting breakthrough→feedback inhibition removal" four-step progressive metabolic engineering system, realizes the "double high yield cooperation" of DHA and astaxanthin, astaxanthin yield is insufficient 0.5 mg / L from wild type jumps to 1506 mg / L, and DHA yield is as high as 34 g / L.

The present disclosure relates to a microorganism having the ability to produce a phytosphingosine derivative and having resistance to cerulenin and / or EGTA, a composition including the same, and a method of producing a phytosphingosine derivative using the same.

Provided here are nucleic acid constructs containing a Mycobacterium tuberculosis genome comprising a mutation (such as a deletion) of the sigH gene (AsigH) and a mutation (such as a deletion) of at least one additional gene, and mutant Mycobacterium tuberculosis encoded by the nucleic acid constructs. Also provided are methods of making such nucleic acid constructs and Mycobacterium tuberculosis mutants, and uses thereof. The construct can stimulate an immune response against Mycobacterium tuberculosis in a subject, and can be used as live attenuated vaccines.

The application provides application of CYP51 protein of Botryosphaeria dothidea in anti-azoles, and belongs to the field of agricultural biotechnology. The application provides application of a reagent for detecting mutation of CYP51 gene sequence or CYP51 protein in Botryosphaeria dothidea in evaluation of azole resistance of Botryosphaeria dothidea. Whether mutation occurs to the 406~408bp position of the CYP51 gene or the 136th tyrosine of the CYP51 protein can effectively evaluate the azole resistance of Botryosphaeria dothidea. The application provides a model of Botryosphaeria dothidea with high azole resistance and a construction method thereof. The model can be used for research and preparation of drugs for prevention and treatment of Botryosphaeria dothidea. The application provides an identification method for azole-resistant Botryosphaeria dothidea and application of the identification method in development monitoring of azole resistance of Botryosphaeria dothidea. The application has important significance for agricultural disease prevention and control and rational use of pesticides, and helps to improve the sustainability and economic benefits of agricultural production.

This invention relates to a microorganism capable of producing plant sphingosine derivatives and resistant to cyanobacterium and / or ethylene glycol bis(3-aminoethyl ether)tetraacetic acid, a composition comprising the microorganism, and a method for producing plant sphingosine derivatives using the microorganism.

The invention relates to a fungal enzymatic composition for degrading plastic products and plastic polymers obtained from the simultaneous cultivation of at least three different species of Trichoderma fungi. Moreover, the present invention also relates to a method for the depolymerization and degradation of plastic-containing waste by the fungal enzymatic composition of the invention.

To provide methods of making and using lipotoxic organoid models.SOLUTION: A method of making a lipotoxic organoid model comprises the steps of contacting a liver organoid with a free fatty acid (FFA) composition, wherein the FFA composition comprises oleic acid, linoleic acid, palmitic acid, or combinations thereof, preferably oleic acid.SELECTED DRAWING: None

The application discloses a 5-ALA high-yield strain mutagenesis screening method, a) after ARTP mutagenesis, 5-ALA strains are respectively coated on culture mediums with 5-ALA concentrations of 20 g / L-50 g / L to obtain first-generation production strains; b) the production strains are subjected to ARTP mutagenesis again, and then are coated on culture mediums with pH values of 5.5-6.5 to obtain 5-ALA production strains. Through two-step ARTP mutagenesis and two kinds of culture environment pressure induction, the production strains with a titer 25% higher than that of the starting strains are obtained, the production cost is reduced, and the production efficiency is improved.

The present invention provides an improved Bacillus cell having reduced expression of a metalloprotease gene. Inactivation of the metalloprotease gene reduces proteolytic degradation of a protein of interest produced by said Bacillus cell and thus, improves the yield of the protein of interest in such production method. The present invention is also directed to a method of inactivating the metalloprotease and the application of this method of inactivation when producing a protein of interest.