495 results about "Sclerotinia sclerotiorum" patented technology

The invention relates to bacillus amyloliquefaciens which has stronger phosphate solubilizing and plant growth promoting functions and has a better preventing and treating effect on various plant diseases. According to the bacillus amyloliquefaciens JCD-H-12 with the phosphate solubilizing, disease preventing and growth promoting functions, disclosed by the invention, the preservation number is CGMCC NO.11856, the preservation date is December 10, 2015, and multiple effects of solubilizing phosphate, preventing diseases, promoting the growth, increasing the yield and the quality, having a broad bacteriostatic spectrum and the like are obtained; a bacterial strain and inoculant of the bacterial strain are capable of effectively preventing and treating powdery mildew, tobacco black shank, botrytis blight, rice blast, cucumber fusarium wilt, tomato early blight, sweet pepper anthracnose, watermelon sclerotinia sclerotiorum, wheat scab, watermelon fusarium wilt, phytophthora capsici and the like; a broad-spectrum antibacterial effect is obtained, the effect is stable and durable, and the bacillus amyloliquefaciens can be developed into a bacterial fertilizer and microbial pesticide preparation which integrates phosphate solubilizing with disease preventing and treating.

The invention provides a biocontrol bacterial strain for preventing and treating plant diseases and its bacterial agent, belonging to the field of biological control. The strains used are Gram-negative bacteria, identified as Lysobacter enzymogenes, and the strain code is OH11. Strain OH11 has no flagella but has slippage, can produce various extracellular hydrolytic enzymes including chitinase, β-1,3-glucanase and protease, and can effectively inhibit the growth of fungi and bacteria. The antibacterial zone diameters of strain OH11 against Sclerotinia sclerotiorum and Phytophthora capsici were both greater than 22.0 mm; the antagonism against potato ring rot was stronger, and the diameter of the inhibition zone reached 50 mm. The OH11 strain was inoculated into the seed tank, cultivated to the logarithmic growth phase, and the seed liquid was connected to the production tank for cultivation. The medium used in the production tank was the same as that of the seed tank. The liquid fermentation adopts aerobic submerged fermentation and fed-batch process, the dissolved oxygen is 15%-20%, the fermentation temperature is 30°C, the fermentation time is 72h, and the initial pH value is 7.5. After the fermentation is completed, the culture solution is taken out of the tank and directly packed into liquid dosage forms with plastic packaging barrels or packaging bottles, or subpackaged into solid dosage forms with peat adsorption packaging bags. The biocontrol strain OH11 can effectively control plant pathogenic fungi, bacteria, nematodes and other diseases, and the overall control effect is 50%-70%. In the greenhouse pot experiment, the control effects of OH11 on pepper blight and tomato bacterial wilt reached 83.6% and 86.4%, respectively. Strain OH11 has the characteristics of broad antibacterial spectrum, high activity, and environmental safety. In today's serious pesticide pollution, zymolysobacterium and its bacterial agent will be a good substitute.

The invention is directed to recombinant antibodies which bind to Sclerotinia sclerotiorum antigens and comprise a single chain variable fragment (scFv). The antigen may be selected from SSPG1d or a portion thereof, aspartyl protease or a portion thereof, or whole Sclerotinia sclerotiorum mycelium. The invention also provides an antibody linked to an anti-fungal polypeptide. The invention extends to nucleic acid sequences encoding the antibodies, and expression vectors comprising the nucleic acid sequences. The invention is also directed to transgenic plants, seeds, tissues or cells transformed with the expression vectors. Methods for producing a transgenic plant that is resistant to Sclerotinia sclerotiorum, and for detecting Sclerotinia sclerotiorum in a biological sample utilizing an antibody which binds to Sclerotinia sclerotiorum antigen, and immunoassay kit for same are also provided.

The invention provides bacillus amyloliquefaciens SZ-60 which is characterized in that the collection number is CGMCC No.8277. The bacterial colony formed by the strain on a beef-extract peptone (NB) medium by virtue of single-cell reproduction is irregular with oyster white color, slight upheaval at the center and wet and semitransparent surface; the microscopy is of a rod shape, and the size is (0.3-0.4)*(3.2-3.3)microns; with flagella, G- and spores, the strain can grow on the beef-extract peptone (NB) medium containing 2-5% of NaCl; the formula of the beef-extract peptone (NB) medium contains 3.0g of beef extract, 10.0g of peptone, 5.0g of NaCl, 17g of agar and 1,000ml of water, and the pH is 6.8-7.2. The bacillus amyloliquefaciens realizes a remarkable antagonistic action on the main pathogenic bacteria causing ginseng root rot, epidemic diseases, sclerotinia sclerotiorum, cylindrocarpon destructans, black spot and damping off. The invention also provides an application of the bacillus amyloliquefaciens SZ-60 in preventing plant fungal diseases, or application in preparing a microbial preparation for preventing plant fungal diseases.

The invention relates to bacillus subtilis Pc3 and a use of bacillus subtilis Pc3 in preparation of a fermentation supernatant for preventing and controlling plant pathogenic fungi, and relates to bacilli. The bacillus subtilis Pc3 has a preservation number of CCTCC NO: M2013470. The bacillus subtilis Pc3 can prevent and control plant pathogenic fungi such as gloeosporium musarum, paecilomyces variotii, sclerotinia sclerotiorum, alternaria longipes, trichoderma viride, rhizoctonia solani and fusaria. A rape sclerotinia sclerotiorum prevention and control pot experiment proves that the bacillus subtilis Pc3 has good biological-prevention and control effects and has prevention and control efficiency of 95.6%. The fermentation supernatant of the bacillus subtilis Pc3 can be prepared by simple processes and has a wide fungus inhibition spectrum and good biological prevention effects.

The invention discloses bacillus subtilis CYY-25 and belongs to the field of microorganisms. The bacillus subtilis CYY-25 is preserved with the preservation number CGMCC No.8995 in China General Microbiological Culture Collection Center and is capable of producing cellulase when salinity ranges from 0% to 15%. The bacillus subtilis CYY-25 is capable of synthetizing IAA and siderophore, dissolving sparingly soluble inorganic phosphorus, promoting plant growth and well inhibiting multiple kinds of plant pathogenic fungi such as fusarium oxysporum, pyricularia oryzae, sclerotinia sclerotiorum, fusarium moniliforme and sclerotinia sclerotiorum, is of great significance to prevention and control of alfalfa root rot and is suitable for being developed and used as an agricultural fungicide.

The invention provides bacillus methylotrophicus SZ-3 and SZ-81, with collection numbers of CGMCC No.8275 and CGMCC No.8276 respectively. The bacillus methylotrophicus SZ-3 has obvious antagonistic effects on pathogenic bacteria causing root rot, black spot and sclerotinia sclerotiorum of ginseng. The bacillus methylotrophicus SZ-81 has obvious antagonistic effects on pathogenic bacteria causing epidemic diseases, root rust rot and black spot of panax ginseng. The invention also provides an application of the bacillus methylotrophicus SZ-3 and SZ-81 to control of fungal diseases of plants or an application to preparation of microbial preparations for controlling fungal diseases of plants.

The present invention provides Bacillus vallismortis SZ-4, which has the preservation number of CGMCC No.8273. According to the present invention, the strain grows on a beef extract-peptone (NB) culture medium in a single cell reproduction growth manner to form bacterial colonies, and the bacterial colonies have characteristics of round shape, milk white color, slightly elevated center, and moist and transparent surface; microscopic examination results show that the Bacillus vallismortis SZ-4 has a rod shape, has flagella, has a size of 0.7-0.82*1.6-2.2 mum, presents the positive Gram staining effect, has spores, and can grow on a beef extract-peptone (NB) culture medium containing 2-5% of NaCl, wherein the beef extract-peptone (NB) culture medium comprises: 3.0 g of a beef extract, 10.0 g of peptone, 5.0 g of NaCl, 17 g of agar and 1000 ml of water, and the pH value is 6.8-7.2; and the Bacillus vallismortis SZ-4 provides significant antagonism effects for pathogenic bacteria causing ginseng root rot, blight, sclerotinia sclerotiorum and botrytis cinerea. The present invention further provides an application of the Bacillus vallismortis SZ-4 in prevention and control of plant fungal diseases or preparation of microorganism preparations for prevention and control of plant fungal diseases.

The invention relates to a traditional Chinese medicine agricultural fungicide for preventing and treating diseases of vegetables and fruits. The traditional Chinese medicine agricultural fungicide has the characteristics of high efficiency, low toxicity, no public nuisance, no pollution, no toxic or side action, wide antibacterial spectrum and long drug effect to vegetable grey mold, sclerotinia sclerotiorum, downy mildew, colletotrichum lagenarium, fruit ring spot, valsa mali and the like. The traditional Chinese medicine agricultural fungicide has dual functions of preventing and treating various diseases caused by fungi, virus and bacteria and can be widely used for preventing and treating the diseases of vegetables and fruits.

The invention discloses application of bacillus amyloliquefaciens in preventing and controlling fungal disease of plants. The application has the advantages that found by study, the bacillus amyloliquefaciens, especially bacillus amyloliquefaciens BSY3 separated and screened from African marigold by an inventor, has obvious antagonistic function on phellinus noxius, pyricularia cannaecola hashioka, sclerotinia sclerotiorum, colletotrichum sp., pestalotiopsissp, fusarium oxysporum f.sp.cubense and the like, and has obvious biological control function and potential application value on preventing and controlling of the fungi diseases due to pathogenic bacteria; the bacillus amyloliquefaciens comes from endophytic bacteria in plants, so that compared with the traditional chemical agent preventing and control method, the safer biological control effect on the fungi diseases is realized; because the bacillus amyloliquefaciens stays in the plant for a long time, the survival relationship with harmonious coexistence and stable development is established with the plant; compared with the chemical agent, the biocontrol function can be more effectively and stably realized.

A construction method for antagonistic Trichoderma-Brassica napus joint adsorption of heavy metals cadmium and zinc in farmland soil in the field of metal adsorption technology, by inoculating bacterial agents prepared by adsorbing heavy genus antagonistic Trichoderma strains to bacteria containing Cd or Zn and Sclerotinia sclerotiorum The soil of the wheat grain culture is further used to cultivate Brassica napus and obtain the Trichoderma-rape symbiont with high efficient adsorption of heavy metals. The invention realizes the dual purposes of bioremediation of heavy metal pollution in farmland soil and treatment of soil-borne diseases, has good repair effect, low cost, is easy to manage and operate, and does not produce secondary pollution, so it has high universal use value in the field.

The invention discloses broad-spectrum disease resisting, growth promotion and stress resisting bacillus capable of preventing and treating tomato gray mold and tomato leaf mold and application of the broad-spectrum disease resisting, growth promotion and stress resisting bacillus. The broad-spectrum disease resisting, growth promotion and stress resisting bacillus is bacillus sp. WXCDD105, and is preserved in China General Microbiological Culture Collection Center (CGMCC); the preservation address is 3, No.1 Yard, Western Beichen Road, Chaoyang District, Beijing, the preservation date is May 25, 2016, and the preservation number is CGMCC No.12496. A biocontrol strain provided by the invention has ultraviolet-resisting and drought-resisting properties, has stronger prevention and treatment effects on the tomato gray mold and the tomato leaf mold, and can inhibit fungal diseases including wilt fusarium of a plurality of types of crops, corn foot root bacteria, rice wilt pathogens, acanthopanax senticosus root rot pathogens, corynespora cassiicolai, colletrichum orbiculare, exserohilum turcicum, tomato spot blight fungi, sunflower sclerotinia sclerotiorum, botrytis cinerea, fusarium oxysporum, verticillium fusarium, alternaria alternata and the like; the broad-spectrum disease resisting, growth promotion and stress resisting bacillus has a growth promotion effect on tomato seeds and seedlings and can also improve the quality of fruits and reduce the rotting rate.

The invention discloses an ultraviolet ray separating greenhouse film. The ultraviolet ray separating greenhouse film disclosed by the invention is an integrated thin film structure which is formed by three layers including an outer layer, a middle layer and an inner layer in a co-extruding and compounding way; and mainly, an ultraviolet inhibitor is introduced into the middle layer of the thin film when polyethylene is machined, so that a formed thin film product can screen ultraviolet light with certain wave-bands in the sunshine, and certain light rays which are harm to the growth of crops can be screened. Particularly, when flowers are planted in a greenhouse, according to the ultraviolet ray separating greenhouse film, and the phenomenon that petals of a rose are dark in color and cluster can be solved, so that the commercial value of products can be improved. The ultraviolet greenhouse film disclosed by the invention can effectively separate more than 98% of ultraviolet light with the length of 290-380nm, is good for controlling the happening of plant diseases and insect pests such as black spot, gray mold, and sclerotinia sclerotiorum of the crops, and is particularly suitable for the growth of the roses.

The invention discloses a breeding method of high-quality double-low disease-resistant high-yield lodging-resistant rape cultivar 'Zhen 2405'. The bred high-quality double-low rape cultivar has the advantages of full and round seed grain, heavy thousand grain weight, black seed grain, good marketability, excellent disease resistance, stable yield, good lodging resistance, good plant-type trait, good maturity performance, consistent maturity and suitability for the mechanized harvest. The method comprises the following steps: A) selecting the early-mature high-yield rapeseed line 'Zhen 9701' as the maternal plant, which has high resistance to Sclerotinia sclerotiorum and lodging and has compact plant-type trait, selecting rape line F1 as the male parent, which has good agronomic characters and high-yield trait, and cross-breeding to obtain a rape line F0; and B) sowing the rape line F0, sowing the excellent individual plants after the two-row sowing of the rape line F0, and self-inbreeding for 6 generations to obtain the high-quality double-low high-yield rape cultivar (Brassica napus) 'Zhen 2405' with excellent resistance to Sclerotinia sclerotiorum and lodging.

The invention belongs to the technical field of agricultural microorganisms, in particular to Bacillus velenzensis and application thereof to biological control. The Bacillus velenzensis disclosed bythe invention is Bacillus velezensis Bv-6, and a preservation number is CCTCC NO:M20191106. The Bacillus velenzensis fermented solutions or powder can effectively inhibit growth of mycelia of important pathogens (Sclerotinia sclerotiorum(Lib.)de Bary, Botrytis cinerea Pers. and Blackleg) on rapes, and germination of conidiophores and ascospores; and through seed soaking treatment of the fermentedsolutions, the seeds of the rapes can be normally germinated and grown, and the edisease resistance of plants can be improved. In addition, the Bacillus velenzensis has the characteristics of being high in proliferation speed, high in stability, high in temperature resistance and free of toxicity. Therefore, the Bacillus velezensis strain Bv-6 has the potential of developing and utilizing the function of the biological control.

The invention provides a method for the genetic transformation of agrobacterium tumefaciens mediated sclerotinia sclerotiorum. The method comprises the following steps: mixing and co-culturing the agrobacterium tumefaciens containing binary vectors and the protoplasts of the sclerotinia sclerotiorum; and screening by using antibiotics to obtain transformants, the transformation efficiency of the method reaches 1/5.9*104 protoplasts. By using the protoplasts of the sclerotinia sclerotiorum as the receptor and using the agrobacterium tumefaciens as the mediator, the method of the invention overcomes the problem of the difficult genetic transformation of sclerotinia sclerotiorum, which is resulted from the multinucleate hypha cells and the difficulty in obtaining a large amount of ascospore, and has the advantages of simple method and stable transformation efficiency. Meanwhile, the invention further provides a simple and effective method for preparing the protoplasts of sclerotinia sclerotiorum.

The invention relates to a method for identifying the resistance of sclerotinia by inoculating an in-vitro stalk of a plant. The method comprises the following steps of: cutting the stalk or branches of the plant down, and putting the stalk or the branches into closed environment in which temperature and humidity are constant to inoculate sclerotinia sclerotiorum; and recording the extension lengths of bacterial plaques to measure the strength of disease resistance of the material. Compared with the conventional method for identifying the resistance of the stalk with sclerotinia, the method has the advantages that the method is performed under the condition of controllable environment, the pathogenetic condition is consistent, and inoculating identification is stable; the method is simple, short in period and high in inoculating efficiency; and inoculated parts are flexible, and a single plant can be identified repeatedly, so the method is suitable for the large-scale inoculating identification of the material.

The invention discloses a gene for resisting sclerotinia rot of cabbage type rape and promoting rape seed germination and application thereof and specifically relates to an AtGDSL gene capable of improving resistance of plants to sclerotinia sclerotiorum biotic stress and promoting rape seed germination. Verification is carried out on the function of the AtGDSL gene in improvement of resistance of plants to sclerotinia sclerotiorum biotic stress and promotion of rape seed germination, and application of the gene is also verified. According to the invention, the exogenous Arabidopis thaliana AtGDSL gene is introduced into a rape plant through a genetic engineering technological means, and after excess expression of the exogenous Arabidopis thaliana AtGDSL gene in the cabbage type rape, the rape plant has substantially improved sclerotinia rot resistance and seed germination capability; and the AtGDSL gene exerts obvious resistance effect on sclerotinia sclerotiorum, can be applied to stress resistance breeding of plants, improves stress resistance of the plants and improves the speed of seed germination, thereby providing excellent breeding materials for large-scale mechanical production of rape.

The invention discloses a sterilization composition containing prothioconazole and azoxystrobin and an application thereof. The sterilization composition takes prothioconazole and azoxystrobin as main effective ingredients. The sterilization composition can be applied to prevention and treatment of diseases of grains, fruit trees and vegetables, has a relatively high synergetic effect, can be used for overcoming and retarding drug resistance of bacteria with high sterilization speed and long duration, and reducing application cost, and has a prevention and treatment effect remarkably superior to that of a single preparation. The sterilization composition can be used for preventing and treating fungous diseases of crops, especially powdery mildew, banded sclerotial blight, fusarium wilt, leaf spot, rust disease, sclerotinia sclerotiorum, net blotch, scald, botrytis, black spot, brown blotch, black shank, glume blight, net blotch, downy mildew and rice blast, and has an effect better than that of a single preparation.

The invention belongs to the plant diseases molecular detection field and relates to the early rapid molecular detection method of sclerotinia rot of colza. The invention is characterized by comprising the collection of weazen rapeseed petals, extraction of total DNA of rapeseed petals, nest-PCR, PCR amplification product analysis, agarose gel electrophoresis and result judgement. If sclerotinia sclerotiorum exists in a sample, a 292bp purpose bank may appear in the agarose gel under ultraviolet light, or else, the sclerotinia sclerotiorum does not exist. The primers of the invention can not only distinguish common plant leaf pathogenic fungi of distance relationship and different categories, such as alternaria genus alternaria sp, fusarium genus fusarium graminearum and coniothyrium genus sclerotinia sclerotiorum sclerotia coniothyrium parasitic fungi, but also can distinguish various fungi of common plant leaf fungal pathogen botrytis also belonging to ascomycotina, discomycete and helotiales with the sclerotinia species; the invention has high sensitivity, can detect the sclerotinia sclerotiorum DNA with the limit of 1fg/Mul, is rapid, and can obtain accurate detection result within 7 hours, however, at least two days or more than 1 week is needed for obtaining the detection result by using the traditional microbiology method.

The invention relates to a heterocycle substituted 1,3,4-oxadiazole compound and a preparation method and application thereof. The compound includes the following steps shown in the general formula (I) in the description. The compound has a good inhibitory effect on pathogenic bacteria and fungi, such as rice white leaf diseases, citrus canker diseases, pepper wilt pathogenic bacteria, blueberry phytophthora lateralis, wheat gibberella saubinetii, potato phytophthora infestans, oilseed rape sclerotinia sclerotiorum, dragon-fruit colletotrichum orbiculare and the like.

The invention discloses an alpine grassland pasture endogenous serratia plymuthica strain GH010 capable of inhibiting various pathogenic bacteria and discloses a microbial agent prepared from the alpine grassland pasture endogenous serratia plymuthica strain GH010 and a preparation method thereof. The alpine grassland pasture endogenous serratia plymuthica strain GH010 and the microbial agent prepared from the same disclosed by the invention have the beneficial effects of having good inhibition effects on the various pathogenic bacteria; the inhibition effect is 67.5% of chili rhizoctonia solani and 69.5% of rape sclerotinia sclerotiorum; the bacteriostasis rates of cotton rhizoctonia solani, eggplant sclerotinia sclerotiorum, tomato botrytis cinerea, fusarium wilt of cucumber, exserohilum turcicum, bipolaris maydis or bipolaristriticola are more than or equal to 57.5%; and the strain has growth-promoting effects on crops including alfalfa and the like, and the environmental protection properties of a microbial pesticide and a microbial fertilizer prepared from the strain are good, the resistance to drugs is unlikely to generate and the safety is good.

The invention relates to a preparation method for a carbazolylv isopropanolamine derivative with the chiral center and application. The structure of the compound is shown in the formula (I), and the compound achieves the better effect of inhibiting xanthomonas oryzae, ralstonia solanacearum, cucumber xanthomonas oryzae, konjak xanthomonas oryzae, xanthomonas citri, grape ulcer bacteria, tomato ulcer bacteria, kiwi fruit ulcer bacteria, apple ulcer bacteria, cucumber botrytis cinerea, chili blight bacteria, sclerotinia sclerotiorum, wheat gibberella saubinetii, potato late blight, blueberry root rotting bacteria and the like.

The invention discloses an aryl triazole compound containing chlorinated cyclopropane and a preparation method and application thereof. The structural formula of the aryl triazole compound containing chlorinated cyclopropane is shown in formula I, wherein X represents C=O or CHOH, and R represents a substituent phenyl group; the substituent in the substituent phenyl group is any one of the following groups: halogen, methyl group, methoxyl group, nitro group, trifluoromethyl group, amino group, and acetyl amino group. The preparation method, provided by the invention, adopts cheap raw materials, and takes a simple reaction route; the synthesized compound has both plant growth regulating activity and bactericidal activity, thereby having a wider application range; in application experiments of the aryl triazole compound containing chlorinated cyclopropane, the plant growth regulating activity shows that the synthesized compound manifests excellent plant growth regulating activity; in application experiments of the aryl triazole compound containing chlorinated cyclopropane, the bactericidal activity test result shows that the compound has excellent growth inhibition effect on sclerotinia sclerotiorum, tomato fungus phytophthora, botrytis cinerea, rhizoctonia solani seedbed, blast fungus, asparagus stem blight, fusarium graminearum, pythium germs, and the like.

The invention discloses pseudomonas aeruginosa JT86 and an application of the pseudomonas aeruginosa JT86 in prevention and treatment of sclerotiniose. The pseudomonas aeruginosa JT86 is screened fromsoil, and the strain is collected in the Guangdong Microbiological Culture Collection Center on September 30, 2019, and has a collection number of GDMCC NO:60799. The strain and a volatile matter thereof can completely inhibit sclerotium germination of rhizoctonia solani, sclerotium rolfsii and sclerotinia sclerotiorum and inhibit growth of sclerotium hyphae, so that the sclerotium hyphae have flakes and wrinkles; the strain also has a good inhibition effect on the rhizoctonia solani, the sclerotium rolfsii, the sclerotinia sclerotiorum, magnaporthe grisea, fusarium oxysporum f. sp. cubense,brassica parachinensis anthracnose, colletotrichum gloeosporioides and colletotrichum capsici; and therefore, the strain can treat recalcitrant soil-borne pathogenic bacteria in soil in a targeted manner, effectively inhibits the sclerotiniose, and has a good application prospect in preparation of a soil treatment agent for preventing and treating soil-borne fungal diseases.