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Alpine grassland pasture endogenous serratia plymuthica strain GH010 and application thereof as well as microbial agent prepared from same and preparation method

A technology of microbial agent and microbial strain, applied in microbial agent and preparation, endophytic Serratia plymuthica strain GH010 and its application field in alpine pasture grassland, can solve the problems of high environmental requirements

Inactive Publication Date: 2013-05-29
GANSU AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, although huge manpower and material resources have been invested in microbial pesticides and microbial fertilizers at home and abroad, chemical pesticides and fertilizers are far more than biological pesticides and fertilizers in terms of quantity; in addition, biological pesticides and fertilizers have higher requirements on the environment

Method used

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  • Alpine grassland pasture endogenous serratia plymuthica strain GH010 and application thereof as well as microbial agent prepared from same and preparation method
  • Alpine grassland pasture endogenous serratia plymuthica strain GH010 and application thereof as well as microbial agent prepared from same and preparation method
  • Alpine grassland pasture endogenous serratia plymuthica strain GH010 and application thereof as well as microbial agent prepared from same and preparation method

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Experimental program
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Effect test

Embodiment 1

[0063] A strain of Serratia plymuthica, whose Latin name is Serratia plymuthica GH010 (CGMCC No.6423), was preserved by the General Microorganism Center of China Committee for the Collection of Microbial Strains, and the preservation number is CGMCC No.6423.

[0064] like figure 1 As shown, endophytic Serratia plymuthica strains in alpine meadow pastures are provided Serratia plymuthica Optical microscope photo of GH010 (CGMCC No.6423).

Embodiment 2

[0066] Serratia plymuthica strain GH010 can inhibit pathogenic bacteria such as capsicum solani blight and Sclerotinia sclerotiorum, endophytic Serratia plymuthica strain Serratia plymuthica The bacteriostatic rate of GH010 (CGMCC No.6423) against Sclerotinia sclerotiorum was 69.5%; the bacteriostatic rate against Capsicum solani blight was 67.5%.

Embodiment 3

[0068] Endophytic Serratia plypolitania strains in pasture grasses in alpine grasslands Serratia plymuthica GH010 (CGMCC No.6423) has inhibitory effect on pathogenic bacteria such as cotton blight, eggplant sclerotinia, tomato cinerea, cucumber wilt, corn leaf spot, corn leaf spot and wheat helminthias, and the inhibition rate At least 57.5%.

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Abstract

The invention discloses an alpine grassland pasture endogenous serratia plymuthica strain GH010 capable of inhibiting various pathogenic bacteria and discloses a microbial agent prepared from the alpine grassland pasture endogenous serratia plymuthica strain GH010 and a preparation method thereof. The alpine grassland pasture endogenous serratia plymuthica strain GH010 and the microbial agent prepared from the same disclosed by the invention have the beneficial effects of having good inhibition effects on the various pathogenic bacteria; the inhibition effect is 67.5% of chili rhizoctonia solani and 69.5% of rape sclerotinia sclerotiorum; the bacteriostasis rates of cotton rhizoctonia solani, eggplant sclerotinia sclerotiorum, tomato botrytis cinerea, fusarium wilt of cucumber, exserohilum turcicum, bipolaris maydis or bipolaristriticola are more than or equal to 57.5%; and the strain has growth-promoting effects on crops including alfalfa and the like, and the environmental protection properties of a microbial pesticide and a microbial fertilizer prepared from the strain are good, the resistance to drugs is unlikely to generate and the safety is good.

Description

technical field [0001] The invention relates to the field of biological control, in particular to an endophytic Serratia plymuthica strain GH010 in pasture grasses in alpine grasslands, an application thereof, a microbial agent prepared by the same, and a preparation method. Background technique [0002] Chemical fungicides can remain in the environment and agricultural products. In addition, pathogens gradually develop resistance to chemical fungicides, and the amount of pesticides used gradually increases, which increases the residual amount. of poisoning. In my country, the safety of agricultural products has attracted people's attention in recent years, especially the problem of excessive pesticide residues in agricultural products, which seriously affects the physical and mental health of the whole people, and at the same time affects the ecological environment and agricultural trade. [0003] With the improvement of people's living standards and the enhancement of env...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/425
Inventor 陈秀蓉杨成德满百膺李振东李鹏荆卓群杨淑君高晓星邹雨坤王辰月
Owner GANSU AGRI UNIV
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