475 results about "Sclerotinia" patented technology

The invention relates to a Bacillus amyloliquefaciense BA-KA3 and application thereof. The Bacillus amyloliquefaciense BA-KA3 is preserved in China General Microbiological Culture Collection Center on October 23, 2013, and has preservation No. CGMCC8342; its on tomato gray mold germs has obviously of antagonistic anti-role, on wheat scab bacteria. The Bacillus amyloliquefaciense BA-KA3 has obvious antagonistic effect on Fusarium graminearum, Rhizoctonia cerealis, Alternaria alternata, Phytophthora capsici, Colletotrichum capsici, Cercospora personata, Alternaria alternate and Garlic sclerotinia disease, and can significantly improve plant growth indexes such as germination rate, and budding rate and root length of seeds,and especially applies to preparation of agricultural preparations for control of tomato diseases due to Botrytiscinerea invasion and has certain growth promotion effect.

The invention is directed to recombinant antibodies which bind to Sclerotinia sclerotiorum antigens and comprise a single chain variable fragment (scFv). The antigen may be selected from SSPG1d or a portion thereof, aspartyl protease or a portion thereof, or whole Sclerotinia sclerotiorum mycelium. The invention also provides an antibody linked to an anti-fungal polypeptide. The invention extends to nucleic acid sequences encoding the antibodies, and expression vectors comprising the nucleic acid sequences. The invention is also directed to transgenic plants, seeds, tissues or cells transformed with the expression vectors. Methods for producing a transgenic plant that is resistant to Sclerotinia sclerotiorum, and for detecting Sclerotinia sclerotiorum in a biological sample utilizing an antibody which binds to Sclerotinia sclerotiorum antigen, and immunoassay kit for same are also provided.

The invention belongs to the field of agricultural chemical bactericides, and discloses an agricultural antifungal composition which is characterized by comprising components A and B, wherein the component A is coumoxystrobin, the component B is a compound selected from triazole bactericides, and the weight ratio of A to B is 1:40-40:1. The composition weakens or retards the generation of resistance of pathogenic fungi to the coumoxystrobin, and avoids the reduction of drug effect resulted from the resistance. Simultaneously, because heterogeneous fungicides form synergism of the composition,the control effect of the composition on diseases is remarkably improved. The composition can be prepared into the formulations of suspending agents, aqueous emulsion, wettable powder or microemulsion, and the like, and is applied to the controlling sclerotinia rot of colza, apple tree canker, rice blast, tomato leaf mold, wheat scab, cucumber scab, tomato anthracnose, cucumber blight, apple ringrot, rice sheath blight, rice bakanae disease, wheat powdery mildew, cucumber powdery mildew, cucumber downy mildew, wheat sheath blight, cucumber gray mold, banana gray pattern disease, and the like.

The invention is directed to recombinant antibodies which bind to Sclerotinia sclerotiorum antigens and comprise a single chain variable fragment (scFv). The antigen may be selected from SSPG1d or a portion thereof, aspartyl protease or a portion thereof, or whole Sclerotinia sclerotiorum mycelium. The invention also provides an antibody linked to an anti-fungal polypeptide. The invention extends to nucleic acid sequences encoding the antibodies, and expression vectors comprising the nucleic acid sequences. The invention is also directed to transgenic plants, seeds, tissues or cells transformed with the expression vectors. Methods for producing a transgenic plant that is resistant to Sclerotinia sclerotiorum, and for detecting Sclerotinia sclerotiorum in a biological sample utilizing an antibody which binds to Sclerotinia sclerotiorum antigen, and immunoassay kit for same are also provided.

The present invention relates to preparation process of strain and preparation for controlling blight of vegetable biologically. One kind of Pseudomonas chlororaphis GP72 is cultured and screened with sweet pepper rhizoid and its attached soil sample and KMB culture medium with antibiotic added, and preserved in the number of CGMCC No. 1748. The freeze preserved strain is activated and amplifying cultured to obtain microbial preparation, which may be used for preventing and controlling blight of chili, cucumber, wax gourd, etc; for preventing and controlling rape sclerotinia rot, cucumber fusarium wilt, water melon fusarium wilt, etc; and as vegetable growth promoter to raise the yield of vegetable.

The present invention relates to an application of anthraquinone derivative as pesticice for controlling plant fungous diseases. The anthraquinone derivative pesticide contains one kind or several kinds of 10 anthraquinone derivative compounds. The anthraquinone derivative compounds have obvious action for preventing and curing several plant diseases, and can be used for preventing and curing economic crop, grain crop, vegetable, fruit tree and melon diseases due to fungi, including sphaerotheca, erysiphe, blumeria, fusarium, sclerotinia, pyricularia, rhizoctonia, colletotrichum, botrytis, verticillium and alternaria.

The invention provides a composite microbial inoculant for controlling plant continuous-cropping diseases, which comprises trichoderma harzianum, bacillus subtilis, and gliocladium roseum, and also comprises phosphate-solubilizing bacteria and potassium-solubilizing bacteria; the accession number of the trichoderma harzianum strain is CGMCC NO. 7861; the accession number of the bacillus subtilis strain is CGMCC NO. 7850; the accession number of the gliocladium roseum strain is CGMCC NO. 9008. The invention also provides a preparation method and an application of the composite microbial inoculant. Through the selection of high-efficient bacterial strains such as disease-preventing trichoderma, bacillus subtilis, gliocladium roseum, phosphate-solubilizing bacteria and potassium-solubilizing bacteria, the composite microbial inoculant of the invention promotes the growth of crops, improves the utilization rate of fertilizers, enhances the stress resistance such as disease resistance and the like, improves the quality of crops, and can control diseases such as root rot, verticillium wilt, damping off, botrytis, epidemic diseases, powdery mildew, sclerotinia rot, seedling-stage damping off, and the like.

The invention provides bacillus methylotrophicus SZ-3 and SZ-81, with collection numbers of CGMCC No.8275 and CGMCC No.8276 respectively. The bacillus methylotrophicus SZ-3 has obvious antagonistic effects on pathogenic bacteria causing root rot, black spot and sclerotinia sclerotiorum of ginseng. The bacillus methylotrophicus SZ-81 has obvious antagonistic effects on pathogenic bacteria causing epidemic diseases, root rust rot and black spot of panax ginseng. The invention also provides an application of the bacillus methylotrophicus SZ-3 and SZ-81 to control of fungal diseases of plants or an application to preparation of microbial preparations for controlling fungal diseases of plants.

The invention discloses a chaetomium globosum FSR-74. The collection unit of the chaetomium globosum FSR-74 is China General Microbiological Culture Collection Center, the collection date of the chaetomium globosum FSR-74 is September 19, 2014 and the collection number of the chaetomium globosum FSR-74 is CGMCC No. 9690. The strain is relatively slow in growth and has light brown colonies and an irregular wave-like edge when subjected to plate culture at 25 DEG C in a potato dextrose agar (PDA) culture medium. FSR-74 ascocarps are supergene and fixed on the surface of a matrix by rhizoids; top hair is wavy, brown and has intervals, and dense wart points are arranged on the surface; 8 ascospores are arranged in an ascus; the ascospores are shaped like lemons and have single apical bud holes. The chaetomium globosum FSR-74 has an efficient broad-spectrum bacteriostatic effect against main pathogenic bacteria causing ginseng rust rot, blight, sclerotinia rot, rust rot, blackspot, sheath blight and gray mold. The invention further provides a biological control mechanism and application of the chaetomium globosum FSR-74 in prevention and treatment of plant fungal diseases, as well as application in preparation of a microbial agent for preventing and treating the plant fungal diseases.

The invention discloses application of psoralen as an agricultural disinfectant or pesticide. The psoralen serving as the pesticide is used for preventing and controlling lepidopterous and homopterous insects, and the psoralen serving as the disinfectant is used for preventing and controlling agricultural fungus diseases and particularly has obvious preventing and controlling effects on sclerotinia rot of colza, cucumber fusarium wilt, rice sheath blight, wheat scab and wheat sharp eyespot. The psoralen serving as the agricultural disinfectant and pesticide is characterized by good effect, broad spectrum, low toxicity, no residue, environmental friendliness and no public hazard, and is particularly favorable for environmental protection.

The invention discloses identification and application of a biocontrol bacillus strain, and belongs to the technical field of agricultural microorganisms. Biocontrol bacillus is bacillus velezensis YJC-9, the strain is already reserved in The China Typical Culture Collection Center of Wuhan university at 11thApril in 2018, and the preservation serial number of the strain is CCTCC NO.M 2018196. Thebiocontrol bacillus strain can prevent and control wheat sharp eyespot, wheat scab, sclerotinia rot of colza, gray mold of strawberries, pepper root rot, kidney bean blight and tobacco alternariaalternata, is a biocontrol bacterium having broad-spectrum resistance, and has good application prospects in agricultural disease prevention and control.

The invention belongs to the technical field of biological pesticide, in particular to screening and identification of a coniothyrium minitans strain with strong parasitism and saprogenicity on sclerotinia sclerotium and an application thereof in a microorganism agent. The coniothyrium minitans ZS-1SB strain is preserved in China Center for Type Culture Collection (CCTCC) with the preservation number being CCTCC NO: M209114. The invention also discloses an open-type solid fermentation cultivation method of the microorganism agent and an application of the coniothyrium minitans ZS-1SB microbial inoculum in the prevention and treatment of sclerotinia of colza. The microorganism agent of the invention has excellent biological prevention effect on plant fungous diseases such as sclerotinia rot of colza and the like.

The invention discloses Bacillus subtilis LNXM37 which is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation date is September 9 th, 2013, and the preservation number is CGMCC No.8141. The Bacillus subtilis LNXM37 can be used for degrading crop straws, and has a function of restraining phytopathogen such as pellicularia sasakii, sclerotinia rot of colza and the like, so that growth of rice and oilseed rape is promoted, and a good application prospect is achieved.

The invention provides bacillus amyloliquefaciens cd5bc. The strain is classified and named as Bacillus amyloliquefaciens cd5bc and is preserved in China Center for Type Culture Collection, the preservation number is CCTCC NO:M 2016232, and the preservation time is April 28, 2016. The invention further provides application of the strain in the aspect of preventing and controlling crop diseases and a biocontrol preparation prepared from the strain. The strain has a very significant preventing and controlling effect on red pepper phytophthora blight, the biocontrol effect reaches up to 100% after inoculation is conducted for 15 days, and the biocontrol effect reaches up to 82.36% after inoculation is conducted for 60 days. The bacillus amyloliquefaciens cd5bc has excellent disease-resistant effects on multiple crop diseases, the bacteriostasis rates of the strain on pathogenic bacteria of red pepper sclerotinia blight, bitter gourd wilt, cucumber wilt, cucumber scabs, rice blast, a rice sheath blight disease, broad bean chocolate spots and broad bean brown spots all reach up to 70% or above, and the bacteriostasis rate of the strain on the broad bean chocolate spots and broad bean brown spots reaches up to 100%.

The invention provides bacillus methylotrophicus with a collection number of CCTCC M2015297. Bacillus methylotrophicus is named bacillus methylotrophicus XK-4. The invention further provides a screening method of bacillus methylotrophicus XK-4, an application of bacillus methylotrophicus XK-4 to inhibition of sclerotinia sclerotiorum, rhizoctonia solani, fusarium solani and fusarium oxysporum and a bacillus methylotrophicus XK-4 agent. Bacillus methylotrophicus XK-4 is a rape rhizosphere microorganism, is safe to human, livestock and crops, is environment-friendly, has a wider antibacterial spectrum, has better inhibitory effects on mycelial growth of common pathogenic bacteria, such as sclerotinia sclerotiorum, rhizoctonia solani, fusarium solani and fusarium oxysporum, has obvious inhibitory effects on mycelial growth of sclerotinia sclerotiorum and causes mycelia deformation. The further prepared agent has obvious control effects on sclerotinia rot of colza, is short in production cycle and has a very extensive application prospect in biological control of plant diseases.

The invention relates to a novel pesticide composition containing prothioconazole and triazoles, which comprises active ingredient A and active ingredient B, wherein active ingredient A is selected from prothioconazole, and active ingredient B is selected from epoxiconazole , Hexaconazole, wherein the weight ratio of active ingredient A to active ingredient B is 1:80 to 80:1, made into wettable powder, water dispersible granule, suspending agent, suspoemulsion, water emulsion, micro Emulsion, used to control powdery mildew, sheath blight, rice false smut, leaf spot, rust, sclerotinia, spotted leaf spot, gray mold, flax spot, scab, black spot, brown spot, For diseases such as black rot, it has the effects of small dosage, resistance to rain erosion, and obvious synergistic effect.

The invention discloses an application of lansiumamide B or salt thereof as a bactericide. The lansiumamide B is amide-type alkaloid and is obtained by separation from wampee stone. The bactericide has broad-spectrum bactericidal activity to multiple plant fungous diseases, can be used individually or mixed with other medicines for treating multiple plant fungous diseases, and has a particularly better treatment effect on paddy rice sheath blight, rape sclerotinia rot, strawberry gray mold. The lansiumamide B can also be used as seed treatment, medical fertilizer, and fresh-keeping of fruits, vegetables and fish.

The invention discloses an oleanolic acid oxime ester derivate compound, a preparation method and an application thereof. The structural formula of the compound is shown in formula I, and in the formula I, R refers to furyl, pyridyl, 1-menaphthyl, 4-chlorophenoxy methyl or phenyl with 1 to 2 substitutional groups; and the substitutional groups in phenyl with 1 to 2 substitutional groups are halogen (such as fluorine, chlorine, bromine and iodine) or nitryl. The preparation method comprises the following steps: 1) performing the esterification reaction between oleanolic oxime subject to benzylation protection and substituted carboxylic acids to prepare an oxime ester compound; and 2) removing benzylation protection on the oxime ester compound by Pd / C-H2 to prepare the compound in the formula I. The bactericidal activity test result shows that CAU2012-A has a favorable effect of inhibiting growth of sclerotinia scleotiorum, phytophthora melongenae, botrytis cintrea, pyricularia grisea, rhizoctonia solani and cotton fusarium wilt.

The invention relates to a method for identifying the resistance of sclerotinia by inoculating an in-vitro stalk of a plant. The method comprises the following steps of: cutting the stalk or branches of the plant down, and putting the stalk or the branches into closed environment in which temperature and humidity are constant to inoculate sclerotinia sclerotiorum; and recording the extension lengths of bacterial plaques to measure the strength of disease resistance of the material. Compared with the conventional method for identifying the resistance of the stalk with sclerotinia, the method has the advantages that the method is performed under the condition of controllable environment, the pathogenetic condition is consistent, and inoculating identification is stable; the method is simple, short in period and high in inoculating efficiency; and inoculated parts are flexible, and a single plant can be identified repeatedly, so the method is suitable for the large-scale inoculating identification of the material.

The invention discloses baciIllus polymyxa KN-03 capable of simultaneously preventing and treating various crop diseases, and the preservation number is CCTCC NO:M2012077. The invention also discloses a culturing method and use of the baciIllus polymyxa. The baciIllus polymyxa can effectively simultaneously prevent and treat crop bacterial wilt, fusarium wilt, verticillium wilt, sclerotinia disease, take-all disease, early blight, gray mold and anthracnose, and has strong sporulation ability, the fermentation liquid spore number can reach 30 billion CFU / ml, at the same time, the baciIllus polymyxa KN-03 also has obvious growth promoting effect, and can significantly increase crop yield.

The invention discloses a gene for resisting sclerotinia rot of cabbage type rape and promoting rape seed germination and application thereof and specifically relates to an AtGDSL gene capable of improving resistance of plants to sclerotinia sclerotiorum biotic stress and promoting rape seed germination. Verification is carried out on the function of the AtGDSL gene in improvement of resistance of plants to sclerotinia sclerotiorum biotic stress and promotion of rape seed germination, and application of the gene is also verified. According to the invention, the exogenous Arabidopis thaliana AtGDSL gene is introduced into a rape plant through a genetic engineering technological means, and after excess expression of the exogenous Arabidopis thaliana AtGDSL gene in the cabbage type rape, the rape plant has substantially improved sclerotinia rot resistance and seed germination capability; and the AtGDSL gene exerts obvious resistance effect on sclerotinia sclerotiorum, can be applied to stress resistance breeding of plants, improves stress resistance of the plants and improves the speed of seed germination, thereby providing excellent breeding materials for large-scale mechanical production of rape.

The invention discloses a cultivation method for interplanting direct-seeding oilseed rape and double-cropping late rice. The cultivation method is characterized by including: selecting one of early and medium maturing varieties including Zhuliangyou 30, Zhuliangyou 819, Zhongzao 25, Zhongzao 35, Zhongjiazao 17 and Luliangyou 996 for early rice; selecting one of early maturing varieties including Wufengyou T025 and Wuyou 308 for late rice; selecting one of Ganyouza No.2, Ganyouza No.5, Fengyou 730, Fengyou 737, Dezayou No.5, Dezayou No.9 and Deyanyou No.19 for later-cropping oilseed rape; interplanting for 5-10 days. The cultivation method is simple in operation and remarkable in benefit, light and temperature resources can be fully used, cropping index can be increased, land consumption and land maintenance are combined to protect and increase soil fertility, common oilseed rape soil-borne diseases such as sclerotinia and the like can be controlled, conflicts of crop rotation arrangement of oilseed rape and rice can be effectively relieved, plant areas of oilseed rape can be directly added, and oilseed rape production capacity of main oilseed rape producing areas adopting a 'rice-rice-rape' triple cropping system can be improved remarkably.

The bactericide composition contains gnotobiotic oxazole and at least one kind of imide bactericides with active component ratio of 0.01-100, and has total active component content of 0.5-95 wt%. The said imide bactericides include dimetachlone, procymidone, iprodione, dichlozoline, chlozolinate, etc. The bactericide composition has obvious synergist effect and may be used in preventing and treating crop diseases caused by pathogenic botrytis cinerea and sclerotinia.

The invention belongs to the plant diseases molecular detection field and relates to the early rapid molecular detection method of sclerotinia rot of colza. The invention is characterized by comprising the collection of weazen rapeseed petals, extraction of total DNA of rapeseed petals, nest-PCR, PCR amplification product analysis, agarose gel electrophoresis and result judgement. If sclerotinia sclerotiorum exists in a sample, a 292bp purpose bank may appear in the agarose gel under ultraviolet light, or else, the sclerotinia sclerotiorum does not exist. The primers of the invention can not only distinguish common plant leaf pathogenic fungi of distance relationship and different categories, such as alternaria genus alternaria sp, fusarium genus fusarium graminearum and coniothyrium genus sclerotinia sclerotiorum sclerotia coniothyrium parasitic fungi, but also can distinguish various fungi of common plant leaf fungal pathogen botrytis also belonging to ascomycotina, discomycete and helotiales with the sclerotinia species; the invention has high sensitivity, can detect the sclerotinia sclerotiorum DNA with the limit of 1fg / Mul, is rapid, and can obtain accurate detection result within 7 hours, however, at least two days or more than 1 week is needed for obtaining the detection result by using the traditional microbiology method.

The invention discloses 1-(1,2,4-triazolyl)ketoxime ether-acylamide disclosed as a chemical structural formula I, wherein R is selected from hydrogen, C1-C2 alkyl group, and C3-C4 straight-chain or branched alkyl group; R1 is selected from hydrogen, C1-C2 alkyl group, and C3-C4 straight-chain or branched alkyl group; and Y is selected from aromatic group or benzyl group. The 1-(1,2,4-triazolyl)ketoxime ether-acylamide disclosed as the chemical structural formula I can be used for preparing bactericide or weedicide for killing Sclerotinia scleotiorum.

The invention relates to a panax notoginseng seedling protection film, and a preparation method and an application thereof. The film provided by the invention is a water-soluble gel prepared by using a water-soluble film-forming material, panax notoginseng polysaccharide, a root growth promoter, a preservative, and a pesticide. With the material, a protection film can be formed on the surface of a panax notoginseng seedling. With the panax notoginseng seedling protection film provided by the invention, water loss of panax notoginseng seedling during a transplanting process can be reduced, and seedling surface skin damage caused by abrasion during transportation or during transplanting can be reduced. Therefore, fungal infection or insect attack caused by damages after transplanting can be reduced, occurrence rate of panax notoginseng root rot, sclerotinia stem rot, sheath blight, anthracnose, and blight can be reduced, and panax notoginseng survival rate can be improved. The panax notoginseng seedling protection film provided by the invention has good physiochemical performances such as film-forming performance, water resistance, air permeability, water permeability, and the like. Therefore, healthy growth of crops can be protected.

An eurotium cristatum LS1 strain is classified and named as EurotiumCristatum LS1 and preserved in China Center for Type Culture Collection on June 17, 2015, and the preservation number is CCTCC NO:M 2015379. The strain LS1 can grow on kudzuvine roots and conduct bioconversion, when the strain LS1 is used for fermenting kudzuvine roots, an obtained fermenting product has stronger inhibiting effects on cowpea anthracnose, tomato early blight, sclerotinia sclerotioru and Chinese cabbage leaf spots, and the inhibiting effects on five putrefactive bacteria of staphylococcus aureus, bacillus cereus, escherichia coli, bacillus subtilis and salmonella are all improved than those before fermentation is conducted. It is shown that a product obtained by fermenting kudzuvine roots through the strain LS1 has broad-spectrum antibacterial activity, can inhibit pathogenic fungi and the putrefactive bacteria to some extent, is expected to be developed into biopesticides to prevent and control crop diseases and is expected to serve as a food sterilizing agent to be used for food preservation.

The invention discloses plant-derived pesticide and a production method thereof. The plant-derived pesticide is prepared from Chinese herbal medicines such as licorice root, phellodendron bark, melia azedarach, cockscomb, radix ranunculi ternati, silktree flower, butterflybush flower, salvia chinensis, fenugreek seed and golden larch bark. A field test proves that the plant-derived pesticide has tobacco black shank prevention and treatment efficiency of 80% or more and has obvious effects better than that of metalaxyl with a concentration of 25%. The plant-derived pesticide has substantial prevention and treatment effects on a plurality of fungous diseases such as downy mildew, sclerotinia rot, powdery mildew, botrytis, anthracnose, epidemic disease, black shank and sheath blight of crops such as fruits, vegetables and paddy rice.

The invention discloses a quinoline compound and a preparation method and application thereof to prevention of plant diseases. The test result shows that the compound has the excellent prevention effects on plant diseases caused by cotton oxysporum, gibberella saubinetii, sclerotinia scleotiorum, rhizoctonia solani and rice blast and pumpkin powdery mildew and cucumber powdery mildew. The quinoline compound is simple in preparation technology, the raw materials are cheap and easy to obtain, the product purity is high, and the quinoline compound can be hopefully developed as a new bactericide.

The invention discloses a Bacillus amyloliquefaciens WH3 strain, and a preparation method and application thereof. The preparation method comprises the following steps: 1, separation and identification of bacteria: separating bacteria resistant to rape sclerotinia rot from rape seedlings, and carrying out 16SrDNA and morphological identification to determine that the WH3 strain obtained by separation is Bacillus amyloliquefaciens; 2, separation, purification and identification of an antifungal active substance Safenour: fermenting the WH3 strain, extracting the antifungal active substance, separating and purifying through a sephadex column, and carrying out MALDI-TOF (matrix-assisted laser desorption / ionization-time of flight ) mass spectrometry on the active antifungal substance to inferthat the substance is a ring type polypeptide; and 3, application of the strain in the preparation of vaccines and immunological adjuvants. After the Safenour used as the adjuvant is mixed with a protein antigen and mice are respectively immunized through oral administration and injection of the mixture, effective body fluid and cellular immune response can be activated, and a high-titer specificantibody can be detected in the blood serum. The Safenour has low production cost and high stability, does not need to be emulsified when mixed with the antigen, and has a better immunoenhancement effect in comparison with Freund adjuvants and cholera toxin B subunits.