122 results about "Sclerotinia" patented technology

The invention belongs to the technical field of biological control, and particularly relates to a pseudomonas flax DY-31 strain, a biocontrol microbial inoculum and application of the biocontrol microbial inoculum. The preservation number of the pseudomonas lini (Pseudomonas lini) DY-31 is CGMCC (China General Microbiological Culture Collection Center) No.34088. The pseudomonas lini (Pseudomonas lini) DY-31 is The pseudomonas flax DY-31 or the biocontrol inoculant thereof is applied to prevention and treatment of plant soil-borne diseases, specifically including peanut black rot, peanut southern blight, peanut sclerotinia rot, peanut leaf rot sclerotinia rot, peanut basal rot and wheat stem basal rot. The pseudomonas flax DY-31 provided by the invention is an important strain resource, can be used for creating a biopesticide preparation, and is used for green prevention and treatment of soil-borne diseases of peanuts and wheat. In addition, the DY-31 strain also has various growth promoting functions, and has important development and application values in the aspects of agricultural biological control and plant growth promoting.

The invention discloses a bactericidal composition containing WML-01 and a triazole compound, and is characterized in that the bactericidal composition is composed of the WML-01 and the triazole compound, and the mass ratio of the WML-01 to the triazole compound is (10: 1)-(1: 10). The bactericidal composition taking the WML-01 and the triazole medicament as effective components has a synergistic effect on prevention and treatment of rice bakanae disease, wheat stem rot, wheat scab, plant root rot and sclerotinia rot of colza, can effectively improve the disease prevention and treatment effect, reduces the pesticide consumption, delays the occurrence of drug resistance, improves the crop yield, and has a good application prospect. The method is safe, effective and economical, and has a wide market application prospect.

The invention discloses a C2 spiro indolone compound as well as a preparation method and application thereof, simple and easily available N-indole connected o-alkynyl nitrobenzene compound is used as a raw material, and the C2 spiro indolone compound is obtained by heating reaction in a solvent under the catalysis of a cheap metal copper catalyst. The C2 spiro indolone compound prepared by the invention has a relatively good inhibition effect on phytopathogen, and can be used for preventing and treating botrytis cinerea, sclerotinia rot of colza and botryosphaeria dothidea; the preparation method does not need to use an expensive metal catalyst, does not need an additional reducing agent, and has the advantages of simple operation, high chemical selectivity, wide reaction substrate range and the like.

The invention belongs to the field of plant biotechnology and plant molecular breeding, and particularly relates to a brassica napus BnabZIP1 gene and application thereof in prevention and control of sclerotinia rot of colza. According to the invention, a gene editing technical vector is constructed aiming at sgRNA of the BnabZIP1 gene, agrobacterium tumefaciens is transformed, then brassica napus is transfected, and a homozygous mutant plant after the BnabZIP1 gene is knocked out has stronger sclerotiniose resistance. Results show that the BnabZIP1 gene can be used as a molecular marker or a genetic engineering improvement target of sclerotiniose resistance of rape, is used for screening and creating disease-resistant rape varieties, and has a good molecular breeding application prospect.

The invention relates to the technical field of pesticides, in particular to a hydrazide derivative containing a 3, 4-dihydroisoquinoline structure, a preparation method and application. The hydrazide derivative containing the 3, 4-dihydroisoquinoline structure is a compound as shown in a formula (I), or a stereoisomer, a tautomer, an isotope derivative and a pesticide acceptable salt thereof. The hydrazide derivative containing the 3, 4-dihydroisoquinoline structure has broad-spectrum inhibitory activity on phytopathogens such as plant pathogenic fungi, especially has excellent inhibitory activity on basidiomycota and ascomycomycota pathogenic fungi such as gaeumannomyces graminis, physalospora piricola, sclerotinia sclerotiorum, botrytis cinerea and rhizoctonia solani, and has broad-spectrum inhibitory activity. The bactericide is expected to be developed into a novel green bactericide which is efficient, safe, economical and environment-friendly.

The invention belongs to the field of rape breeding, and particularly discloses a sclerotinia rot resistance related gene BnaCLE20 of rape and application of the sclerotinia rot resistance related gene BnaCLE20 in rape breeding. The sequence of the BnaCLE20 gene is as shown in SEQ ID NO. 1, and the coded protein is as shown in SEQ ID NO. 2; the BnaCLE20 gene of the brassica napus is identified to be capable of regulating the sclerotinia rot resistance of the brassica napus for the first time, a molecular network for regulating the sclerotinia rot resistance of plants is enriched, and the BnaCLE20 gene plays an important role in researching a molecular mechanism formed by the disease resistance of the plants. The overexpression of the BnaCLE20 gene disclosed by the invention can reduce the content of active oxygen, malondialdehyde and oxalic acid in rape leaves infected by sclerotinia sclerotiorum; meanwhile, the activity of catalase in the rape leaves infected by sclerotinia sclerotiorum is enhanced; the sclerotiniose resistance of the oilseed rape is remarkably improved, and the method has important significance in guaranteeing the high and stable yield of the oilseed rape by improving the sclerotiniose resistance of the oilseed rape.

The application discloses application of BoDMR1 and BoDMR6 genes in cultivating disease-resistant broccoli varieties, an expression vector and a cultivating method, and relates to the technical field of plant molecular biology. A nucleotide sequence of a broccoli BoDMR1 gene is shown in SEQ ID No: 7, or a nucleotide after gene mutation of the nucleotide sequence shown in SEQ ID No: 7; and a nucleotide sequence of a broccoli BoDMR6 gene is shown in SEQ ID No: 8, or a nucleotide after gene mutation of the nucleotide sequence shown in SEQ ID No: 8. Through gene editing on endogenous BoDMR1 and / or BoDMR6 genes of plants, mutant plants showing resistance to diseases such as sclerotinia, black rot and alternaria blight can be obtained. Therefore, the application is beneficial to providing good resources for cultivating disease-resistant broccoli varieties.

This invention discloses an oxyindene compound containing substituted pyridine and its application as a fungicide. The oxyindene compound containing substituted pyridine has broad-spectrum fungicidal activity, especially effective in controlling rice sheath blight, apple tree rot, rapeseed sclerotinia stem rot, wheat scab, pepper blight, cucumber anthracnose, tomato gray mold, corn curvature blight, rice blast, tomato early blight, wheat take-all, and wheat stem rot. It can be used as a fungicide for the prevention and control of the above-mentioned plant diseases.

This invention discloses a nanoRNA delivery system and its application in the control of Sclerotinia sclerotinia disease, relating to the field of biotechnology. The preparation method of the nanoRNA delivery system includes the following steps: mixing chitosan, sucrose, and aspartic acid uniformly and then subjecting them to a hydrothermal reaction to obtain a quantum dot-chitosan degradation product complex; mixing the quantum dot-chitosan degradation product complex with double-stranded RNA to obtain the nanoRNA delivery system; the double-stranded RNA is used to silence Sclerotinia sclerotinia virulence-related transcription factor genes. The nanoRNA delivery system provided by this invention can effectively protect RNA and exhibits a very high inhibitory effect on Sclerotinia sclerotinia in plants, providing a novel technical solution for the green and precise control of Sclerotinia sclerotinia disease.

The application provides a Rhodiola kirilowii root rot harmless prevention and control method and relates to the technical field of plant disease prevention and control. The Rhodiola kirilowii root rot harmless prevention and control method is characterized by the following steps: after seed disinfection, the seed is treated by immersion in a solution of a Yunnan sclerotia of Sclerotinia spore powder extract, and a fermentation substrate of Bacillus subtilis and Bacillus amyloliquefaciens is used, and then the roots are irrigated with a solution of a Yunnan sclerotia of Sclerotinia spore powder extract and a Trichoderma harzianum liquid. The method can effectively prevent and control the Rhodiola kirilowii root rot, reduce the use of chemical pesticides, reduce pesticide residues, and improve the safety of planting.

This invention discloses a fungicide composition containing WML-01 and triazole compounds. The fungicide composition is characterized by comprising WML-01 and triazole compounds in a mass ratio of 10:1 to 1:10. The fungicide composition provided by this invention, with WML-01 and triazole agents as active ingredients, exhibits a synergistic effect in the control of rice bakanae disease, wheat stem rot, wheat scab, plant root rot, and rapeseed sclerotinia stem rot. It can effectively improve disease control efficacy, reduce pesticide usage, delay the development of pesticide resistance, and increase crop yield. It is safe, effective, and economical, and has broad market application prospects.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to a transcription factor BnC05.PLATZ01 gene for regulating and controlling sclerotinia rot resistance of rape and application of the transcription factor BnC05.PLATZ01 gene. A transcription factor BnC05.PLATZ01 gene capable of enhancing sclerotiniose resistance of rape is screened, and the nucleotide sequence of the transcription factor BnC05.PLATZ01 gene is as shown in SEQ ID NO: 1; the sequence of the protein coded by the gene is as shown in SEQ ID NO: 2. According to the method, a pGWB418-BnC05. PLATZ01 overexpression strain is obtained by virtue of agrobacterium tumefaciens mediated transformation. Sclerotinia sclerotiorum inoculation identification on a transgenic material finds that the sclerotinia rot resistance of a BnC05. PLATZ01 overexpression strain is enhanced, which indicates that the BnC05. PLATZ01 is a positive regulation factor of the sclerotinia rot resistance of the rape and has a positive regulation effect in the sclerotinia rot resistance of the rape, and the sclerotinia rot resistance of the rape can be remarkably improved by overexpressing the gene.

The invention discloses a method for preventing and treating sclerotinia rot of colza by antagonistic trichoderma strains, and relates to the technical field of agricultural disease prevention and control, and the method comprises the following specific steps: S100, strain screening and mutagenesis: separating trichoderma strains from rhizosphere soil of colza in different environments, primarily screening strains with antagonistic action on sclerotinia sclerotiorum by a plate confrontation method, and carrying out mutagenesis on the strains with antagonistic action on sclerotinia sclerotiorum; carrying out chemical mutagenesis treatment on the primarily screened strains, carrying out confrontation culture on the primarily screened strains and sclerotinia sclerotiorum again, screening out high-yield antagonistic substances with larger inhibition zones and mutant strains with strong stress resistance, and culturing to obtain trichoderma fermentation liquor; by obtaining the trichoderma strains which can keep efficient antagonism and strong stress resistance under different environmental conditions, the stability of the trichoderma strains on disease control is enhanced, so that the rape can effectively resist invasion of sclerotiniose under different environmental conditions in the growth process, and the disease resistance of the rape is improved. And antagonistic active substances are extracted to prepare a biological fertilizer carrier, and the biological fertilizer carrier is applied to the planting process of oilseed rape, so that the growth and reproduction of sclerotinia sclerotiorum are effectively inhibited.

The application discloses a cyclohexane-fused masked 2-pyridone compound and a preparation method and application thereof, and relates to the technical field of organic synthesis. The preparation method comprises the following steps: taking a simple and easily obtained substituted cyclohexane cyclopentadienyl ester as raw material, taking iodobenzene diacetic acid as an oxidant, taking ammonium carbamate as a nitrogen source, taking magnesium oxide and water as additives, taking chloroform and 2,2,2-trifluoroethanol as mixed solvents, and reacting at room temperature to obtain the cyclohexane-fused masked 2-pyridone compound. The application has the advantages of mild reaction condition, simple operation, high yield, wide reaction substrate range and the like, and does not need to use a metal catalyst, does not need to be heated and does not need to be operated in a water-free and oxygen-free manner. The cyclohexane-fused masked 2-pyridone compound prepared by the application has a good inhibiting effect on plant pathogenic fungi, and can be used for the prevention and treatment of tomato gray mold, cereal scab, rapeseed sclerotinia, pepper pythium blight and grape cavity disease.

This invention relates to the field of medicinal chemistry, specifically to a capsaicin-like compound containing isopropanolamine, its preparation method, and its application. This type of compound has a structure as shown in general formula (I). The invention uses capsaicin and isopropanolamine as pharmacophores, connecting the two pharmacophores with different bridging chains to synthesize a series of capsaicin-like derivatives containing isopropanolamine. These compounds exhibit excellent inhibitory effects on plant pathogenic bacteria such as rice bacterial blight, citrus canker, rice bacterial leaf streak, and kiwifruit canker; and plant pathogenic fungi such as cucumber gray mold, pepper wilt, rapeseed sclerotinia rot, eggplant verticillium wilt, wheat scab, potato late blight, blueberry root rot, grape coccidioidomyces, dragon fruit anthracnose, and rice sheath blight, providing an important scientific basis for the research and development of new pesticides.

The application belongs to the field of plant biotechnology and plant molecular breeding, and particularly relates to Brassica napus BnabZIP1 Gene and application thereof in prevention and control of oilseed rape sclerotinia stem rot BnabZIP1 The sgRNA of the gene is used to construct a gene editing vector, and the agrobacterium is transformed, then the Brassica napus is transfected, and it is found that BnabZIP1 The homozygous mutant plant after the gene knockout has stronger resistance to the sclerotinia stem rot. BnabZIP1 The gene can be used as a molecular marker or a genetic engineering improvement target for the resistance to the sclerotinia stem rot, is used for screening and creating the disease-resistant oilseed rape varieties, and has a good molecular breeding application prospect.

The invention relates to a quinoline hydrazone compound containing a hydrazone structure as well as a preparation method and application of the quinoline hydrazone compound, and relates to the technical field of chemical engineering and pesticides. According to the invention, 5-aminoquinoline is used as a raw material to synthesize a series of quinoline hydrazone compounds containing a hydrazone structure; the prepared compound can effectively prevent and treat plant fungal diseases including cucumber gray mold, potato late blight, sclerotinia rot of colza, tomato gray mold, wheat scab, rice blast, kiwi fruit phomopsis and rice sheath blight disease.

The invention belongs to the technical field of microorganisms, and particularly relates to a sclerotinia sclerotiorum pathopoiesis and reproduction dual-deletion strain SD3-9V, a fungicide and application of the fungicide. The invention provides a sclerotinia sclerotiorum pathopoiesis and reproduction dual deletion strain (Sclerotinia sclerotiorum) SD3-9V, which is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC M 2026255. The sclerotinia sclerotiorum pathopoiesis and reproduction dual-deletion strain SD3-9V provided by the invention has no pathogenicity, and sclerotium formation ability is deleted, so that the sclerotinia sclerotiorum can be used as a plant vaccine for preventing sclerotinia rot, and has multiple advantages of high biological safety, reduction of sclerotinia rot morbidity and promotion of crop growth and flowering. The invention provides an effective way for providing a safer and more reliable microbial agent for preventing and treating sclerotiniose.

The invention relates to the technical field of medicinal chemistry, and discloses a thymol derivative as well as a preparation method and application thereof. The thymol ethyl, propyl, butyl azole and piperazine derivative provided by the invention is used as a bacteriostatic agent for resisting phytopathogen and is used for preparing pesticides; the prepared pesticide can be used for preventing and treating plant diseases such as cucumber damping-off, rice damping-off, cotton damping-off, watermelon damping-off, sclerotinia rot of colza, botrytis cinerea, cucumber gray mold, grape gray mold, wheat scab, rice blast, wheat sharp eyespot, wheat stem rot, sweet potato soft rot and lily wilt.

The application provides application of Brassica napus gene BnDTX19 in sclerotinia prevention and control, and is obtained by creating transgenic rapeseed to change the disease resistance of rapeseed material. The application constructs the super-expression and RNAi transgenic rapeseed of BnDTX19, firstly discloses the positive regulation function of the gene on sclerotinia resistance, provides the application of the BnDTX19 gene in obtaining high-sclerotinia-resistant rapeseed material by creating super-expression rapeseed, and the application of the BnDTX19-RNAi rapeseed in obtaining sclerotinia-resistant rapeseed material with weakened sclerotinia resistance. The BnDTX19 gene product provided by the application has the function of transporting the key pathogenic factor oxalic acid of sclerotinia to promote disease resistance, and is a new gene resource suitable for creating and breeding new sclerotinia-resistant rapeseed materials and new varieties.

The application discloses a kind of phenylacrylamide derivatives and preparation method and purposes thereof, belong to organic synthesis chemistry and pesticide technical field.Phenylacrylamide derivatives are prepared by condensation reaction from cinnamic acid or its derivatives and benzimidazole derivatives.Biological activity test shows that the compounds exhibit different degrees of inhibitory activity on rice sheath blight pathogen, barley root rot pathogen, wheat scab pathogen and brassica rapa sclerotinia pathogen, and can be used for preparing fungicide for preventing and treating agricultural plant diseases.

This application discloses a strain of *Priestia taiwanensis* W178 and its application in controlling soil-borne fungal diseases. This strain was deposited at the China Center for Type Culture Collection (CCTCC) on December 29, 2025, with accession number CCTCC NO:M 20253047. The strain exhibits significant antagonistic activity against 11 soil-borne pathogenic fungi, including *Sclerotinia sclerotiorum* and *Fusarium graminearum*, with an inhibition rate reaching up to 91.10%. This application also establishes a fermentation process for this strain, resulting in a spore count of 19.3 × 10⁻⁶ after fermentation. 8 CFU / mL. Pot experiments showed that the fermentation broth, diluted 10 times, was significantly effective in controlling rapeseed sclerotinia stem rot and could effectively inhibit the spread of lesions. This application is the first to discover the broad-spectrum biocontrol potential of *Priscilla taiwanensis* and provides an active strain and its fermentation product that can be developed into microbial agents, bio-organic fertilizers, or compound microbial fertilizers, suitable for the green control of various soil-borne fungal diseases such as rapeseed sclerotinia stem rot and wheat stem rot.

The application provides a cycloalkyl-substituted triazolopyridine amide compound and a preparation method and application thereof, and belongs to the technical field of pesticides. The cycloalkyl-substituted triazolopyridine amide compound provided by the application introduces an unsubstituted or substituted cyclohexyl group or an unsubstituted or substituted cyclopentyl group on a triazole ring, has excellent inhibitory effect on pepper Phytophthora capsici Leonian, and has an EC 50 as low as 0.3 micromole / L; and the cycloalkyl-substituted triazolopyridine amide compound and derivatives thereof also have broad-spectrum inhibitory activity on rice Helminthosporium leaf spot fungus, wheat Helminthosporium leaf spot fungus, brassica sclerotinia, wheat Gibberella zeae, wheat take-all fungus, tomato botrytis cinerea, potato late blight fungus, pepper Phytophthora capsici Leonian, tomato early blight fungus, rice bacterial wilt fungus, potato dry rot fungus, cucumber anthracnose fungus and rice rice blast fungus, and have a good application prospect in the preparation of anti-plant pathogenic fungus drugs.

The application belongs to the technical field of plant genetic engineering, and particularly relates to a pectin methylesterase inhibitor BnPMEI8 gene for regulating resistance of rape to sclerotinia stem decay and application. A pectin methylesterase inhibitor BnPMEI8 gene capable of enhancing host cell wall strength and induced to express by sclerotinia stem decay is screened, the nucleotide sequence of the gene is shown as SEQ ID NO:1, and the protein sequence coded by the gene is shown as SEQ ID NO:2. A BnPMEI8-CRISPR / Cas9 knockout mutant strain is obtained by using agrobacterium-mediated transformation. It is found by inoculating the transgenic material with sclerotinia that the BnPMEI8 mutant strain has enhanced resistance to sclerotinia stem decay, indicating that BnPMEI8 is a negative regulatory factor of rape to sclerotinia stem decay, plays a negative regulation role in the resistance of rape to sclerotinia stem decay, and the lack of expression of the gene can significantly improve the resistance of rape to sclerotinia stem decay.

The invention relates to a methylene ester bridge-containing compound as well as a preparation method and a bactericide application thereof. The compound has a structure as shown in a formula (I). The compound disclosed by the invention has broad-spectrum bactericidal activity on various plant fungal diseases, can be used for preventing and treating various plant fungal diseases independently or by being compounded with other medicaments, and particularly has an excellent preventing and treating effect on rice sheath blight disease, cucumber gray mold, wheat scab and sclerotinia rot of colza.

The invention discloses application of a BnGAE1 gene in regulation and control of sclerotinia rot resistance of brassica napus, and belongs to the technical field of plant genetic engineering. The gene BnGAE1 capable of enhancing the sclerotinia rot resistance of the brassica napus is separated and cloned from the brassica napus, and the nucleotide sequence of the gene is as shown in SEQ ID NO. 1. Experimental verification shows that the resistance of the BnGAE1 (KO) strain to sclerotinia sclerotiorum is remarkably enhanced, and the incidence rate is reduced by 25.46% after inoculation; physiological and molecular detection shows that the expression quantity of defense related genes (such as PR1 and PDF1.2) in a mutant strain is up-regulated, SA (salicylic acid) and JA / ET (jasmonic acid / ethylene) signal channels are activated, and meanwhile, excessive ROS (reactive oxygen species) is effectively removed, so that the redox balance is maintained. The invention provides a new gene target and a high-efficiency molecular breeding technical path for improvement of sclerotinia rot resistance of colza, and has an important application prospect.

The invention provides a sclerotinia sclerotiorum mutant strain SEEN10 and application of the sclerotinia sclerotiorum mutant strain SEEN10 in monocotyledon yield increase, and belongs to the technical field of microorganism application. The invention provides a Sclerotinia sclerotiorum mutant strain SEEN10, wherein a gene knocked out of the Sclerotinia sclerotiorum mutant strain SEEN10 is a xylan hydrolase gene SsXyl2 (SS1G03618), and the gene knocked out of the Sclerotinia sclerotiorum mutant strain SEEN10 is a xylan hydrolase gene SsXyl2 (SS1G03618). When the mycelium suspension of the strain SEEN10 is applied to seed dressing treatment of wheat seeds, compared with a wild sclerotinia sclerotiorum strain, the yield increase of monocotyledons can be more remarkably promoted, and the pathogenicity of the strain SEEN10 to oilseed rape is greatly reduced. The growth promoting advantage of the strain SEEN10 provides a technical path for green agriculture.

The utility model relates to the technical field of gibberella fermentation, in particular to a waste residue treatment device for gibberella fermentation, which comprises a support table, an outer cylinder is mounted at the top of the support table in a penetrating manner, a cover plate is placed at the top of the outer cylinder, a support frame is connected to the center of the top of the cover plate, and a variable frequency motor is mounted on the support frame in a penetrating manner. The output end of the variable frequency motor and the interior of the outer cylinder are jointly connected with a processing part. According to the utility model, the variable frequency motor runs slowly, and meanwhile, the telescopic end of the electric telescopic rod II extends out to further open the electromagnetic valve on the discharge pipe; when the telescopic end of the second electric telescopic rod corresponds to the arc-shaped notch, the follow-up variable frequency motor runs at a high speed, so that liquid in the gibberella fermentation waste residues is thrown out and discharged from the discharging pipe. The gibberellic acid fermentation waste residues are crushed by the main heating crushing rod and the auxiliary heating crushing rod, and the gibberellic acid fermentation waste residues are dried by the heating column, the main heating crushing rod and the auxiliary heating crushing rod.

The present disclosure provides compositions and methods for producing plants having resistance to diseases caused by Sclerotinia (e.g., Sclerotinia sclerotiorum) such as, for example, white mold. The methods involve expressing in plants a heterologous nucleic acid associated with increased resistance to Sclerotinia. Additionally, compositions and methods useful in producing, identifying and / or selecting plants and plant materials comprising the heterologous nucleic acid associated with increased resistance to Sclerotinia are provided.

The invention relates to application of BnaPRT6 protein and a coding gene thereof in regulating sclerotiniose resistance of cruciferous crops. The BnaPRT6 protein is selected from any one of the following components: an amino acid sequence as shown in SEQ ID NO: 1, a nucleotide sequence as shown in SEQ ID NO: 2, a nucleotide sequence as shown in SEQ ID NO: 3 and a nucleotide sequence as shown in SEQ ID NO: 4; an amino acid sequence as shown in SEQ ID NO: 3; an amino acid sequence as shown in SEQ ID NO: 5; the amino acid sequence is shown as SEQ ID NO: 7. The invention discloses the function of the BnaPRT6 gene in regulation and control of sclerotinia rot resistance of colza for the first time, and provides a new molecular target for disease-resistant breeding of the colza. The BnaPRT6 gene is knocked out in a targeted manner through a CRISPR / Cas9 technology, and a novel rape germplasm with remarkably enhanced sclerotiniose resistance can be obtained. The obtained mutant plant is normal in growth and development, which indicates that the BnaPRT6 knockout does not affect the main agronomic traits of the rape while improving the disease resistance.