151 results about "Molecular mechanism" patented technology

The invention relates to the technical field of plant genetic engineering, and particularly discloses an alfalfa salt-alkali-resistant and low-temperature composite stress-resistant gene MsNCED5 and application of encoded protein thereof in improving salt-alkali-resistant and low-temperature composite stress-resistant capability of plants. The nucleotide sequence of the gene disclosed by the invention is as shown in SEQ ID NO.1, the full length is 1833 bp, and 610 amino acids are encoded. According to the invention, an MsNCED5 overexpression vector is constructed and medicago sativa is converted, so that an MsNCED5-OE transgenic line is successfully created, and the MsNCED5 gene expression quantity of the MsNCED5-OE transgenic line is obviously increased compared with that of a wild type. Under the salt-alkali and low-temperature composite stress, the transgenic plant has stronger stress tolerance compared with the wild plant, which indicates that the overexpression of the MsNCED5 gene improves the resistance of the plant to the salt-alkali and low-temperature composite stress. The MsNCED5 gene disclosed by the invention provides an important theoretical basis for researching an alfalfa stress resistance molecular mechanism and breeding.

The invention provides a design method of a protein variant structure based on molecular dynamics, and belongs to the technical field of molecular dynamics simulation. A protein-standard nucleic acid compound ground molecule system and a protein-nonstandard sequence compound ground molecule system are respectively constructed, and based on a molecular dynamics simulation technology, protein conformation stability, low-energy conformation states, main change processes, collaborative movement and key residues of the protein-standard sequence system and the protein-nonstandard sequence system are compared, so that protein conformation stability and low-energy conformation states of the protein-standard nucleic acid compound ground molecule system and the protein-nonstandard sequence compound ground molecule system are obtained. The molecular mechanism of protein-nucleic acid interaction is analyzed, the recognition behavior of the protein on the standard nucleic acid sequence and the activation process of the standard nucleic acid sequence on the corresponding function of the protein are understood from the molecular level, and finally the protein variant with more related functions is designed.

The invention belongs to the field of citrus breeding, and discloses application of a citrus CsERF113 gene in regulation and control of plant fruit abscission. It is found for the first time that the CsERF113 gene can induce plant separation layer formation and fruit abscission, which has a great promotion effect on molecular mechanism research of abscission regulation of plants including organs such as fruits and the like; the citrus CsERF113 gene cloned by the invention has a remarkable induction effect on citrus fruit abscission, which is of great significance to cultivation of overwintering non-abscission late-maturing citrus varieties through a gene editing technology.

The invention relates to the technical field of biology, in particular to application of a biological product expressed by an EsPTI15 gene to regulation and control of tiller number. The nucleotide sequence of the EsPTI15 gene is shown as SEQ ID NO: 1, it is found for the first time that the tiller number of EsPTI15 gene overexpressed siberian wildrye is remarkably increased compared with wild siberian wildrye, and the result proves that overexpression of the EsPTI15 gene can positively regulate and control the plant tiller development process. The discovery not only enriches the molecular mechanism theory of plant tillering regulation, but also verifies that the EsPTI15 gene has the application potential of increasing the tillering number of the plant, and provides key theoretical support and gene resources for subsequent technical research and development of introducing the EsPTI15 gene through gene operation, increasing the tillering number of forage grass and further realizing biomass improvement.

The invention relates to the field of plant genetic engineering, in particular to a rice OsDNG1 protein, a coding gene and application of the rice OsDNG1 protein to regulation and control of grain shape and grain weight. According to the invention, the OsDNG1 gene is knocked out through a CRISPR / Cas9 gene editing technology, so that the length of rice grains can be increased by 4.10%-4.62%, and the thousand grain weight can be increased by 10.86%-11.49%. And if the gene is over-expressed, the grain length is reduced by 1.14%-1.32%, and the thousand grain weight is reduced by 8.24%-8.56%. In the re-sequenced indica rice variety, the indica rice variety without the OsDNG1 gene type is longer in grain length and larger in length-width ratio compared with the variety with the gene type. The invention not only provides a key target for analyzing the molecular mechanism of rice grain morphogenesis, but also provides important theoretical basis and technical support for molecular design breeding of rice grain shape and grain weight, and has remarkable agricultural application value.

The invention relates to the field of gene engineering and molecular breeding, and discloses application of a ZmTCP5 gene in regulation and control of heat resistance of corn. According to the invention, three types of knockout mutants of the gene are obtained by using a CRISPR / Cas9 technology, and three overexpression materials are created. At normal temperature (25-28 DEG C), the material has no obvious phenotypic difference with a wild type; however, under high temperature stress (45 DEG C), the ZmTCP5 knockout mutant has enhanced heat resistance, reduced reactive oxygen species (ROS) accumulation, and significantly increased ROS scavenging enzyme activity, chlorophyll content and photochemical efficiency; on the contrary, the ZmTCP5 overexpression strain is more sensitive to heat stress, which indicates that the ZmTCP5 negatively regulates the heat resistance of the corn. The research provides a theoretical basis for analyzing a heat-resistant molecular mechanism of the corn, and also provides an important gene resource for creating a new germplasm of the heat-resistant corn.

The invention belongs to the technical field of gene engineering, and particularly relates to application of corn zma-miR319 and / or a target gene of the corn zma-miR319 in regulation and control of corn stem rot resistance. According to the application, sequencing data of small RNA, a degradation group and a transcriptome are integrated, a miRNA family member zma-miR319 is identified, and the resistance of corn to stem rot can be positively regulated and enhanced; the target gene ZmMYB74 is a core regulation gene of an Fg response module, the stem rot resistance of a transgenic plant over-expressing the ZmMYB74 is weakened, and lignin deposition is reduced; the resistance is obviously enhanced by knocking out or inhibiting ZmMYB74, the ZmMYB74 is used as a transcription inhibition factor, the expression of a lignin synthesis related gene ZmCAD is negatively regulated and controlled, and the separation of the disease-resistant gene ZmMYB74 not only contributes to the cultivation of a persistent disease-resistant corn variety, but also deepens the understanding of a stem rot resistance molecular mechanism.

The invention discloses an application of a rice OsWRKY44 gene in plant salt tolerance regulation and control. The invention discovers and verifies that OsWRKY44 is a gene for positively regulating and controlling the salt tolerance of rice in the seedling stage, and provides a new target for analyzing a rice salt-tolerant molecular mechanism. The overexpression of the OsWRKY44 can significantly improve the tolerance of the rice to high-concentration NaCl (200mM) treatment in the seedling stage, is suitable for improving the salt tolerance character of the existing rice variety, and has important theoretical and practical significance for cultivating the salt-tolerant plant variety.

The invention belongs to the technical field of gene cotton resistance breeding, and particularly relates to application of cotton GhGSTF8 and GbGSTF8 genes in cotton verticillium wilt resistance. According to the application, the expression level of the GhGSTF8 gene in leaves and receptacles of cotton is relatively high through qRT-PCR (Quantitative Reverse Transcription-Polymerase Chain Reaction) detection; the expression level of the GbGSTF8 gene in leaves and stems of cotton is high. VIGS experiments show that after the GhGSTF8 gene is silenced, a new plant body shows an obvious susceptible character characteristic to verticillium wilt infection. After the GbGSTF8 gene is silenced, the silenced plant shows the character characteristic of resistance. Based on the research result, a certain application foundation can be laid for the molecular mechanism research of verticillium wilt resistance and the cultivation of new varieties of novel verticillium wilt-resistant plants.

The invention provides an application of an OsINV2 gene in regulation and control of rice cold stress resistance. A nucleotide sequence of the OsINV2 is shown as SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3 or SEQ ID NO: 4. By revealing the key regulation and control effect of the vacuole sucrose invertase gene OsINV2 in rice low-temperature stress response, the molecular mechanism that the gene affects the cold resistance of plants by regulating the sugar metabolism balance in vacuoles is illustrated for the first time. The expression of the OsINV2 is regulated in a targeted manner through molecular marker-assisted breeding or a gene editing technology, so that the low-temperature survival rate in the seedling stage and the cold resistance in the booting stage of the rice can be remarkably improved, glumous flower degeneration and pollen abortion caused by low temperature are reduced, and meanwhile, the limitation of the cold resistance of varieties on geographical distribution is broken through; a theoretical support and a technical path are provided for breeding and adaptive popularization of cold-resistant rice varieties.

The invention relates to the field of genetic engineering breeding and molecular breeding, and particularly discloses application of ZmMIR319A in regulation and control of heat resistance of corn. According to the invention, corn ZmMIR319A is knocked out by using a gene editing technology, three different types of gene knockout mutants are created, and a ZmMIR319A overexpression material is created at the same time. At the normal growth temperature (25-28 DEG C) of corn, the ZmMIR319A knockout mutant and the overexpression material do not show temperature sensitivity to the wild type, but at the high temperature (45 DEG C), compared with the wild type, the ZmMIR319A mutant is more sensitive to high temperature stress, and the sensitivity of the ZmMIR319A overexpression material to the high temperature stress is reduced, which indicates that the ZmMIR319A positively regulates and controls the high temperature stress resistance of the corn. The invention provides reference for studying the high-temperature-resistant stress molecular mechanism of the corn, and provides potential gene resources for cultivating excellent new germplasm of the high-temperature-resistant corn.

The invention relates to the technical field of plant genetic engineering, and provides application of Leymus chinensis LcCBP60b gene in regulation and control of plant tiller number and biological yield. It is found for the first time that the LcCBP60b gene can improve the tiller number and the biological yield of leymus chinensis, and the LcCBP60b gene has important guiding significance on cultivation of high-yield and high-quality leymus chinensis varieties; the invention also clarifies the molecular mechanism of the LcCBP60b gene in regulation and control of the tiller number and the biological yield of Leymus chinensis, further analyzes the molecular module for regulating and controlling the tiller number and the biological yield of plants, can enrich the corresponding molecular mechanism, and provides a new breeding direction for breeding high-yield and high-quality varieties.

The invention belongs to the technical field of agricultural biology, and provides a soybean drought stress tolerance gene and application thereof, and the nucleotide sequence of the gene comprises a CDS sequence as shown in SEQ ID NO: 1; the nucleotide sequence has at least 90% of homology with the sequence shown in SEQ ID NO: 1 and is used for coding a protein with a drought stress response function; one or more nucleotides are substituted, deleted or inserted in the sequence as shown in SEQ ID NO: 1, and a nucleotide sequence of a protein with a drought stress response function is coded; according to the application disclosed by the invention, the direct association between the soybean gene Gm12g076200 and the drought stress tolerance is clarified, and the deletion of the soybean gene Gm12g076200 causes a soybean drought sensitive phenotype, so that the research blank of the gene on the stress response function is filled; the deletion of the gene can significantly reduce the tolerance of the soybean to drought stress, and the expression quantity is significantly increased under the drought stress, so that a key candidate gene is provided for research of a soybean drought-resistant molecular mechanism.

The application relates to the field of genetic engineering breeding and molecular breeding, and particularly discloses ZmMIR319A Application of a gene in regulating a corn leaf angle. ZmMIR319A The gene nucleotide sequence is shown in SEQ ID NO. 1. The application uses a CRISPR / Cas9 gene editing technology to carry out site-specific knockout of a gene in corn ZmMIR319A, Three different types of gene knockout mutants are created, and ZmMIR319A Overexpression materials are created. It is found that, compared with a control, the leaf angle of a leaf on an ear of the knockout mutant is smaller, and the leaf angle of a leaf on an ear of the overexpression plant is increased, which indicates that ZmMIR319A ZmMIR319A The gene can positively regulate a corn leaf angle. The application lays a theoretical foundation for researching a molecular mechanism of a corn leaf angle, and simultaneously provides new molecular markers and gene resources for molecular design breeding and cultivating new excellent corn germplasm with a small leaf angle and a dense planting characteristic.

The invention provides an application of an NFKBIA gene in identification of salt tolerance of fish. Through experiments, the inventor finds that the expression quantity of the NFKBIA gene in the grass carp group subjected to salinity stress is obviously higher than that of the grass carp group without salinity stress, and the relative expression quantity of the NFKBIA gene in the freshwater grass carp group and the relative expression quantity of the NFKBIA gene in the salinity training grass carp group are obviously different; the invention reveals that the NFKBIA gene can obviously influence the tolerance of the grass carp to the salt stress, and the NFKBIA gene has a negative regulation effect on the salt stress coping process of the grass carp, and the expression quantity of the NFKBIA gene is closely related to the salt tolerance of the grass carp. The invention discovers that the expression quantity of the NFKBIA gene has significant correlation with the salt tolerance of grass carp for the first time, provides a new target for the research of fish salt tolerance molecular mechanism, enriches the understanding of fish salt stress adaptation mechanism, and provides molecular biology basis for the breeding of fish salt tolerance varieties and the optimization of culture environment.

The invention belongs to the technical field of plant genetic engineering and soybean breeding, and discloses application of a GmCDPKb gene in regulation and control of the number of soybean symbiotic nodules, the nucleotide sequence of the GmCDPKb gene is shown as SEQ ID NO.1, and the amino acid sequence of encoded protein is shown as SEQ ID NO.2. According to the invention, a GmCDPKb gene mutation and overexpression soybean stable genetic transformation plant is constructed, and character investigation shows that the nodulation number of a mutant plant is obviously lower than that of a wild type, and the nodulation number of an overexpression plant is obviously higher than that of the wild type. The result shows that the GmCDPKb gene plays a role in regulating and controlling the number of soybean nodules. The invention reveals that the GmCDPKb gene is a key regulatory factor for regulating the number of soybean root nodules symbiotic nodules, the number of soybean nodules can be stably and obviously regulated by regulating the expression quantity of the gene, and important gene resources and germplasm materials are provided for analyzing a soybean symbiotic nitrogen fixation molecular mechanism and cultivating a new efficient nitrogen fixation soybean variety.

The invention relates to application of a ZmbZIP68 protein and a coding gene thereof in regulation and control of heat resistance of corn. It is found that the corn ZmbZIP68 gene can positively regulate the heat resistance of plants, and the heat resistance of the plants can be effectively improved by overexpressing the expression quantity of the ZmbZIP68 gene. The discovery of the heat-resistant function of the ZmbZIP68 gene provides a new gene target and resource for cultivating heat-resistant plant varieties, has important significance on research on a heat-resistant molecular mechanism of corn, and lays a certain theoretical foundation for research on a high-temperature stress response mechanism and a molecular mechanism for resisting adverse environments of plants.

The invention relates to the field of genetic engineering breeding and molecular breeding, and particularly discloses application of a ZmMIR319A gene in regulation and control of an included angle of corn leaves. The nucleotide sequence of the ZmMIR319A gene is as shown in SEQ ID NO. 1. According to the invention, ZmMIR319A in corn is knocked out at a fixed point by using a CRISPR / Cas9 gene editing technology, three different types of gene knockout mutants are created, and a ZmMIR319A overexpression material is created at the same time. Compared with a control group, the ZmMIR319A has the advantages that the leaf included angle of the spike leaves of the knockout mutant is reduced, and the leaf included angle of the spike leaves of the overexpression plant is increased, so that the ZmMIR319A can positively regulate and control the corn leaf included angle. The invention lays a theoretical foundation for researching a corn leaf included angle molecular mechanism, and provides a new molecular marker and a new gene resource for molecular design, breeding and cultivation of excellent new corn germplasm with small leaf included angle and close planting resistance.

The application discloses application of a SdC2'H gene of Saposhnikovia divaricata and a method for increasing coumarin content in Saposhnikovia divaricata, and belongs to the technical field of bio-agriculture. The SdC2'H gene of Saposhnikovia divaricata and the biological material containing the SdC2'H gene are applied to the regulation of coumarin synthesis in Saposhnikovia divaricata. The method for increasing coumarin content in Saposhnikovia divaricata comprises the steps of increasing the expression amount and / or activity of the SdC2'H gene in Saposhnikovia divaricata. The SdC2'H gene is successfully cloned from Saposhnikovia divaricata for the first time, and a gene overexpression vector is constructed. The SdC2'H gene is positively regulated to control the synthesis of coumarin for the first time, and overexpression of the SdC2'H gene can significantly increase the product content. This not only deepens the understanding of the molecular mechanism of related biosynthesis, but also lays an important foundation for efficient production of high-value coumarin by using genetic engineering technology.

The invention discloses a diaphorina citri effector protein DcPDI1 and an application thereof. The amino acid sequence of the diaphorina citri effector protein DcPDI1 is as shown in SEQ ID NO. 1, and the nucleotide sequence of the diaphorina citri effector protein DcPDI1 is as shown in SEQ ID NO. 2. After the diaphorina citri DcPDI1 is knocked out, the egg laying amount and the developmental duration of the diaphorina citri are remarkably reduced, and it is indicated that the DcPDI1 plays an important role in growth and development of the diaphorina citri. The diaphorina citri effect protein DcPDI1 can be a new molecular target spot, plays a key role in activating defensive response of host plants and helping diaphorina citri to eat, and provides a new insight for a molecular mechanism of interaction between diaphorina citri and plants.

This invention discloses a molecular event visualization method and electronic device, proposing the concept of a molecular film. The method acquires the topological structure file of the drug molecule-target protein and the original trajectory file generated after molecular dynamics simulation of the drug molecule-target protein; based on the original trajectory file and topological structure file, it obtains the keyframe sequence of the molecular dynamics simulation of the drug molecule-target protein; it monitors the keyframe sequence to identify key event sequences of the drug molecule-target protein, and annotates the key event sequences to obtain annotated key event sequences; and it renders the annotated key event sequences to visualize and output the generated rendering data as molecular events, significantly improving the efficiency and depth of understanding molecular mechanisms of action in drug discovery and optimization.

This invention provides a chalcone isopentenyltransferase gene. GiPT16 This invention relates to the GiPT16 protein, amplification primer set, and applications, belonging to the field of biogenetics technology. It is based on *Glycyrrhiza inflata* (GiPT16 protein, amplification primer set, and applications). Glycyrrhiza inflata Using whole-genome sequencing data and a reverse genetics strategy, the key aromatic isopentenyltransferase GiPT16, involved in the isopentenylation modification of chalcone active ingredients, was successfully identified and functionally characterized. This enzyme was confirmed to specifically catalyze the biosynthesis of psoralen and glycyrrhizin C using DMAPP as a donor. It is the first chalcone isopentenyltransferase characterized in *Glycyrrhiza inflata*, filling a gap in the study of key enzymes in the chalcone isopentenylation metabolic pathway of this species and providing important evidence for further elucidating the molecular mechanisms of quality formation in *Glycyrrhiza inflata* medicinal materials.

The invention belongs to the technical field of plant genetic engineering and disease-resistant breeding, and particularly relates to a construction method of soybean GmLNK2 gene four mutants and application of the soybean GmLNK2 gene four mutants in soybean disease-resistant breeding, by means of a CRISPR / Cas9 technology, wild soybean WS82 is used as a material, three target spots are designed for GmLNK2a, GmLNK2b, GmLNK2c and GmLNK2d genes of a GmLNK2 gene family, and simultaneous knockout is performed to obtain a mutant plant. Compared with a wild type WS82, the plant height of a mutant plant is obviously reduced, the length of a main root is obviously shortened, and the leaf area of three-out compound leaves is obviously reduced, which indicates that the mutant plant is more sensitive to pathogenic bacteria in a root rot disease nursery, the susceptibility degree is more serious, and growth and development are obviously inhibited, the key effect of the GmLNK2 gene family in soybean root rot resistance is clarified, and the application of the GmLNK2 gene family in soybean root rot resistance is developed. Important gene resources and technical support are provided for soybean disease-resistant molecular mechanism research and disease-resistant variety cultivation.

In response to the current problem of "insufficient heat-resistant corn germplasm resources and limited location and cloning of key genes", the present invention provides an application of corn nuclear transcription factor NF-YA9 in improving plant stress resistance, including high temperature stress, salt stress and cold stress, and the plants include corn, Arabidopsis, rice, etc. <h2 style=";text-align:left;direction:ltr">zmnf‑ya9 Mutant and overexpressing transgenic Arabidopsis lines were validated <h2 style=";text-align:left;direction:ltr"> ZmNF‑YA9 The function of regulating high temperature stress in maize was analyzed preliminarily. <h2 style=";text-align:left;direction:ltr"> ZmNF‑YA9 The molecular mechanism of regulating high temperature stress in corn provides genetic resources and theoretical basis for the creation of new germplasm of heat-resistant corn and the cultivation of new varieties. <h2 style=";text-align:left;direction:ltr"> ZmNF‑YA9 It also has the function of positively regulating salt stress and cold stress in corn.

The present invention belongs to the field of biotechnology, relates to new applications of genes, and particularly refers to proteins, genes, their editing vectors and applications for controlling the symbiotic nitrogen fixation efficiency of soybeans. The present application has discovered two genes of soybeans GmNAS1 , GmNAP1 and one transcription factor GmNFYC10a , and obtained the gene sequences of the three through reverse genetics means, and successfully isolated, cloned and verified them by using the soybean hairy root transient transformation system, stable transgenic plants, PCR reactions and gene sequencing. The soybean genes GmNAS1 , GmNAP1 and the transcription factor GmNFYC10a control the symbiotic nitrogen fixation efficiency of soybeans through their interactions with each other, revealing the GmNAS1 , GmNAP1 and GmNFYC10a molecular mechanism for improving the nitrogen fixation efficiency of root nodules by regulating the flow direction of PEP.

This invention belongs to the field of microbial fermentation technology and discloses a method and application for improving the production of staminodesmotic acid (Sclerotium sclerotiorum) based on dynamic osmotic pressure regulation. The method uses *Sclerotium sclerotiorum* as the production strain and employs a synergistic strategy of initial culture medium component optimization, precise osmotic pressure regulation during fermentation, and dynamic feeding to enhance synthesis. Utilizing the dual characteristics of sucrose as a carbon source and osmotic pressure regulator, and the osmotic pressure regulating effect of KCl, the osmotic pressure during fermentation is precisely controlled between 1050-1300 mOsmol / kg. This activates the MAPK-CWI signaling pathway and FKs2 gene expression, directionally promoting staminodesmotic acid synthesis and increasing staminodesmotic acid yield. This invention, by optimizing the initial carbon source and inorganic salt concentration, precisely regulating osmotic pressure during fermentation through feeding, and combining molecular mechanism analysis, achieves a significant increase in staminodesmotic acid yield, reduces industrial production costs, and provides technical support for the large-scale industrial production of staminodesmotic acid.

This invention belongs to the field of molecular biology technology, specifically involving MaMADS35 Application of genes in regulating postharvest carotenoid accumulation in bananas. This invention uses 'Zhongre No. 1' banana fruit as experimental material to investigate the changes and molecular mechanisms of carotenoid metabolism under different temperature conditions, and screens and identifies genes related to banana carotenoid synthesis with nucleotide sequences as shown in SEQ ID NO:1. MaMADS35. The verification results show that MaMADS35 Can be with MaPSY1 The promoter binding of the gene regulates carotenoid production at the transcriptional level, and can be used to regulate postharvest carotenoid accumulation in bananas. Furthermore, this invention provides a formula containing... MaMADS35 Genetic biomaterials or MaMADS35 MaMADS35 The application of the gene-encoded MaMADS35 protein in regulating postharvest carotenoid accumulation in bananas provides a theoretical basis and scientific support for optimizing postharvest preservation and storage of bananas, improving fruit quality, and promoting industrial development.

This invention discloses a major gene for controlling cold tolerance in rice. bHLH150 This invention relates to the field of plant genetic engineering technology, specifically the major gene for controlling cold tolerance. bHLH150 The nucleotide sequence is shown in SEQ ID No:1. After CRISPR / Cas9 editing mutations in rice, the transgenic plants exhibited significantly reduced cold tolerance and became cold-sensitive; while those expressing or overexpressing the gene... bHLH150 The rice exhibits a high survival rate at low temperatures, thus improving its cold resistance. This invention relates to the major cold-resistance gene. bHLH150 Research on the molecular mechanisms regulating cold tolerance in rice is of significant theoretical and practical importance for the breeding of new cold-tolerant rice varieties. It also has broad application prospects and a promising market outlook in the agricultural field.

The invention provides application of a rice OsABI3 gene in reduction of rice cadmium accumulation, and relates to the technical field of gene engineering. The nucleotide sequence of the gene is shown as SEQ ID NO.1. The application comprises the following steps: directionally editing the OsABI3 gene by using a CRISPR / Cas9 technology, constructing a recombinant vector, transforming the recombinant vector into a rice callus by using an agrobacterium-mediated method, and screening to obtain a mutant plant. The rice OsABI3 gene is cloned and analyzed for the first time, the nucleotide sequence of the gene is disclosed, a new perspective is provided for illuminating an unknown molecular mechanism of rice for regulating and controlling Cd transportation, and the invention also proves that the OsABI3 gene has the capability of regulating and controlling Cd accumulation of rice; and tissue anatomy and fluorescent staining experiments prove that the OsABI3 has the capability of limiting radial transportation of Cd ions in a root system.

The present invention provides a method for improving rice yield tms5 The method for the sterility threshold temperature of two-line sterile lines comprises the following steps: TMS5 CRISPR / Cas9 gene editing targets T501 and T502 were designed 22bp and 57bp downstream of the gene translation start site ATG, respectively, and gene editing vectors TMS501 and TMS502 were constructed to transform rice varieties to achieve TMS5 Site-directed mutation of genes to obtain different multiple alleles tms5 Gene frameshift mutant material. Experiments have shown that in the same genetic background, the T501 target site editing tms5-1 The sterility starting temperature of the frameshift mutants was significantly higher than that of the T502 target. tm5-2 Mutants. The method provided by the present invention can quickly obtain rice with different sterility threshold temperature phenotypes under the same genetic background. tms5 Mutants can overcome the interference of different genetic backgrounds and provide a basis for studying rice tms5 The molecular mechanism and genetic regulatory network of the starting temperature of two-line sterility provide new ideas.