18 results about "Molecular mechanism" patented technology

This invention discloses a molecular event visualization method and electronic device, proposing the concept of a molecular film. The method acquires the topological structure file of the drug molecule-target protein and the original trajectory file generated after molecular dynamics simulation of the drug molecule-target protein; based on the original trajectory file and topological structure file, it obtains the keyframe sequence of the molecular dynamics simulation of the drug molecule-target protein; it monitors the keyframe sequence to identify key event sequences of the drug molecule-target protein, and annotates the key event sequences to obtain annotated key event sequences; and it renders the annotated key event sequences to visualize and output the generated rendering data as molecular events, significantly improving the efficiency and depth of understanding molecular mechanisms of action in drug discovery and optimization.

This invention belongs to the field of microbial fermentation technology and discloses a method and application for improving the production of staminodesmotic acid (Sclerotium sclerotiorum) based on dynamic osmotic pressure regulation. The method uses *Sclerotium sclerotiorum* as the production strain and employs a synergistic strategy of initial culture medium component optimization, precise osmotic pressure regulation during fermentation, and dynamic feeding to enhance synthesis. Utilizing the dual characteristics of sucrose as a carbon source and osmotic pressure regulator, and the osmotic pressure regulating effect of KCl, the osmotic pressure during fermentation is precisely controlled between 1050-1300 mOsmol / kg. This activates the MAPK-CWI signaling pathway and FKs2 gene expression, directionally promoting staminodesmotic acid synthesis and increasing staminodesmotic acid yield. This invention, by optimizing the initial carbon source and inorganic salt concentration, precisely regulating osmotic pressure during fermentation through feeding, and combining molecular mechanism analysis, achieves a significant increase in staminodesmotic acid yield, reduces industrial production costs, and provides technical support for the large-scale industrial production of staminodesmotic acid.

This invention discloses a major gene for controlling cold tolerance in rice. bHLH150 This invention relates to the field of plant genetic engineering technology, specifically the major gene for controlling cold tolerance. bHLH150 The nucleotide sequence is shown in SEQ ID No:1. After CRISPR / Cas9 editing mutations in rice, the transgenic plants exhibited significantly reduced cold tolerance and became cold-sensitive; while those expressing or overexpressing the gene... bHLH150 The rice exhibits a high survival rate at low temperatures, thus improving its cold resistance. This invention relates to the major cold-resistance gene. bHLH150 Research on the molecular mechanisms regulating cold tolerance in rice is of significant theoretical and practical importance for the breeding of new cold-tolerant rice varieties. It also has broad application prospects and a promising market outlook in the agricultural field.

This invention relates to the fields of genetic engineering breeding and molecular breeding, and discloses a novel gene for resistance to ear rot. ZmER5 This invention relates to the gene and its application in improving resistance to maize ear rot. The nucleotide sequence of this gene is shown in SEQ ID No. 1, and the amino acid sequence of the encoded protein is shown in SEQ ID No. 2. Through genome-wide association analysis, this invention identified a gene encoding the NAC transcription factor that is significantly associated with resistance to maize ear rot. ZmER5 Using CRISPR / Cas9 gene editing technology, two different homozygous editing types were created. size5 Knockout mutant ,size5 The mutant showed significantly reduced resistance to ear rot, indicating ZmER5 Genes positively regulate maize ear rot resistance. This invention lays the theoretical foundation for elucidating the molecular mechanism of maize ear rot resistance, and also provides new gene resources for breeding superior new maize germplasm with ear rot resistance characteristics.

The application provides a preparation method of a Rhodobacter sphaeroides CsCobT and CsCobN protein complex in vitro, and steps are as follows: constructing an expression vector pET-28a(+)‑Cs CobN , pET-28a(+)‑SUMO‑Cs CobT ; expressing and purifying His-CsCobN protein; preparing a non-tagged CsCobT protein; mixing the purified His-CsCobN protein and the non-tagged CsCobT protein to form a protein complex; separating the complex through affinity chromatography; and refining the complex through gel filtration chromatography. The application adopts a strategy of double vectors, step-by-step purification, in-vitro assembly and multi-dimensional verification, can obtain a CsCobT and CsCobN protein complex with high purity and uniform conformation, and the experimental process can be accurately controlled, the result is specific, and provides high-quality protein materials for subsequent molecular mechanism research and application development of cobalt-chelating enzyme.

This invention belongs to the field of plant genetic engineering and relates to the application and method of reducing the expression of the Zm7649 gene in improving plant stress resistance. This invention utilizes an EMS mutant with premature termination of expression. Zm7649-ems Heterologous overexpression with Arabidopsis thaliana Zm7649-OE Verified Zm7649 The function of negatively regulating salt stress and high temperature stress was studied, and its preliminary analysis was performed. Zm00001d027649 The molecular mechanism regulating stress involves the interaction between Zm7649 and a maize catalase (Zm4848), with ubiquitination degrading Zm4848 and reducing its protein stability. This invention provides new genetic resources and theoretical basis for maize stress-resistance breeding or germplasm innovation.

The application belongs to the field of plant genetic engineering, and relates to a BrSTM16 gene and application. The nucleotide sequence of the BrSTM16 gene is shown in SEQ ID NO. 1. The application provides the BrSTM16 gene and application which can solve the problem of the molecular mechanism of the response of the white cabbage type winter rape to low-temperature stress and can cultivate new cold-resistant varieties (lines).

The application relates to the technical field of biology, and particularly relates to a kind of N 6 ,N 6 - Dimethyladenosine and its application. (1) The application locks the key metabolite N 6 ,N 6 - Dimethyladenosine with significant anti-GCRV activity through metabolomics. Experiments prove that the metabolite not only significantly reduces virus load at the in vivo level, but also extremely significantly down-regulates the expression of VP7 at the cell level, thereby inhibiting the assembly and replication of GCRV from the source, and having a clear anti-virus molecular mechanism. (2) The application discloses a new biological function of N 6 ,N 6 - Dimethyladenosine in the field of aquatic anti-virus, and proves that the metabolite can be used as an efficient anti-virus active molecule to inhibit GCRV replication. The application provides important theoretical support and factual basis for developing new, green and safe anti-grass carp hemorrhagic disease drugs and feed additives.

The application discloses a molecular mechanism automatic prediction method and system based on a complex compound system, relates to the technical field of bioinformatics analysis, and comprises the following steps: constructing an integrated analysis platform, configuring a visual workflow, performing full-process automatic analysis, and generating a result and a structured report. The application forms a unified integrated analysis platform by integrating a multi-source data interface, multiple independent analysis modules, a dynamic module adaptation mechanism and multi-source data collaborative verification logic, realizes end-to-end process connection from compound information input to structured report generation, does not need manual intervention in data transmission and format conversion, solves the problems of dispersion, process fragmentation and complicated operation of traditional tools, improves the overall efficiency of molecular mechanism prediction in early drug research and development, and adopts a drag-and-drop visual workflow configuration interface to support users in combining independent analysis modules and flexibly configuring data transmission paths, so that the configuration difficulty of a complex analysis process is reduced.

The application belongs to the field of animal genetic engineering and molecular biotechnology, and particularly relates to FDXR The application of the expression activator of the gene in the preparation of a product for resisting porcine epidemic diarrhea virus infection. FDXR The nucleotide sequence of the gene is shown as SEQ ID NO. 1. The research result of the application shows that the expression level of the gene will affect the replication level of PEDV. FDXR When the expression of the gene in a host cell is inhibited, the replication of PEDV can be significantly promoted and the titer of the virus is increased. FDXR When the FDXR is overexpressed in the host cell, the replication of PEDV can be significantly inhibited and the titer of the virus is decreased. FDXR The experimental result not only shows that the gene can be used as a new target for the development of an antiviral drug for PEDV, but also provides a tool and material for the research on the molecular mechanism of the gene in the regulation of the replication of a pathogenic microorganism in a cell. FDXR The application belongs to the field of animal genetic engineering and molecular biotechnology, and particularly relates to

This invention relates to the field of plant genetic engineering technology, specifically disclosing a major gene for cold tolerance in rice seedlings, its encoded protein, and its applications. The major gene... RGA4L The nucleotide sequence is shown in SEQ ID No:1. This invention relates to a major gene controlling cold tolerance in rice seedlings. RGA4L After the expression of the gene was reduced in rice, the T3 transgenic plants showed a significant decrease in cold tolerance and became cold-sensitive; the gene was expressed or overexpressed. RGA4L Rice varieties with this gene exhibit high survival rates at low temperatures and demonstrate cold tolerance. Research on the molecular mechanism regulating cold tolerance in rice seedlings has significant theoretical and practical implications for the breeding of new cold-tolerant rice varieties. It also has broad application potential and market prospects in the agricultural field.

This invention discloses tomatoes SlARP6 The application of genes as negative regulators in improving the cold tolerance of tomatoes, the aforementioned SlARP6 The nucleotide sequence of the protein-coding region of the gene is shown in SEQ ID NO: 1, and the application method is through knockout. SlARP6 The gene significantly enhances the low-temperature resistance of tomatoes. This invention provides key gene resources for the breeding of new low-temperature resistant tomato varieties, clarifying... SlARP6 This study explores the application value of genes as negative regulators of low-temperature resistance; it also lays an important theoretical foundation for elucidating the molecular pathways of tomato's response to stress signals and the molecular mechanisms of tolerance to adverse environments, and provides new ideas for molecular breeding practices to improve plant stress resistance.

This invention discloses the ZmGRAL1 protein, its encoding gene, and its applications, which are related to the stage-specific regulation of maize leaf color phenotype. It belongs to the fields of plant genetic engineering and breeding technology. This invention screened and obtained a maize leaf color mutant. gral1 ,Should gral1 The mutant exhibits a significant albino phenotype before V5, followed by regreening; agronomic traits such as plant height, 100-grain weight, and grain number per ear are not different from the wild type, making it a leaf color mutant with application value. For analysis... gral1 The molecular mechanism of mutant phenotype formation was isolated in this invention using map-based cloning technology. ZmGRAL1 Genes, and verified through gene knockout. ZmGRAL1 Genes that regulate the development of maize leaf color. ZmGRAL1 Genes can regulate chloroplast development and stage-specific regulation of maize leaf phenotype, and can be used as maternal marker genes to efficiently identify false hybrids through leaf color during the seedling stage.

The application belongs to the field of plant genetic engineering, and relates to a BrCUC2 gene and application, wherein the nucleotide sequence of the BrCUC2 gene is shown as SEQ ID NO. 1. The application provides the BrCUC2 gene and application which can solve the problem of a molecular mechanism of a response of a white cabbage type winter rape to low-temperature stress and can cultivate a cold-resistant new variety (line).

This invention belongs to the field of biology, specifically relating to the application of the rubber tree HbEIN3-1 gene in regulating the biosynthesis of natural rubber. The HbEIN3-1 gene described in this invention binds to the promoter of the HbCPT gene, enhancing the promoter activity of the HbCPT gene. This indicates that HbEIN3-1 participates in the regulation of HbCPT expression at the transcriptional level, and that the expression level of HbCPT is positively correlated with the expression level of HbEIN3-1, thus directly participating in the regulation of natural rubber biosynthesis. This invention elucidates the molecular mechanism by which HbEIN3-1 regulates natural rubber biosynthesis, providing a new regulatory gene for increasing natural rubber yield.

This invention provides the application of the FgFd1 gene in regulating the growth, reproduction, pathogenicity, and stress resistance of *Fusarium graminearum*, belonging to the field of genetic engineering technology. The nucleotide sequence of the FgFd1 gene is shown in SEQ ID NO.2, and the amino acid sequence of the protein encoded by the FgFd1 gene is shown in SEQ ID NO.1. The *Fusarium graminearum* FgFd1 gene knockout mutant (ΔFgFd1) obtained by this invention exhibits slower growth rate, reduced conidia number, decreased pathogenicity, and reduced stress resistance compared to the wild-type (WT) strain. This invention provides a technical basis for exploring the molecular mechanisms of sporulation and pathogenicity of *Fusarium graminearum*, and provides potential molecular targets for developing disease control measures targeting sporulation inhibition, showing broad application prospects in plant fungal disease research.

This invention discloses a genetically engineered product based on the Elavl4 / miR-301a-5p / Nurr1 axis and its applications, belonging to the fields of genetic engineering and neuroprotection. Addressing the technical shortcomings of unclear molecular mechanisms, limited intervention methods, and lack of standardized detection tools for atrazine (ATR)-induced dopaminergic neuronal damage, this invention provides the first experimental verification that Elavl4–miR-301a-5p–Nurr1 is a key ceRNA regulatory axis in ATR neurotoxicity. The regulatory mechanism is clarified as follows: ATR exposure downregulates Elavl4, releasing its sponge-like adhesion to miR-301a-5p, thereby inhibiting Nurr1 expression and leading to neuronal damage. This invention provides a genetically engineered product targeting this regulatory axis, which can significantly reverse ATR-induced decreased cell viability, increased apoptosis, and PD-like behavioral defects in animals; it also provides a biomarker composition containing Elavl4, miR-301a-5p, and Nurr1 for assessing ATR exposure risk and the degree of neuronal damage. This invention has an innovative mechanism and strong targeting, and can be applied to the development of neuroprotective drugs, the construction of high-throughput screening models and toxicity assessment, showing great potential for translation.

The present invention relates to the use of a combination of two or more of a D2 agonist, an adenosine A2A receptor antagonist, a histamine H3 antagonist or inverse agonist, a mGluR5 receptor antagonist, or a nicotinic α4-β2 and / or α7 receptor agonist to increase D2 dopaminergic molecular signaling in the striatum for the treatment of psychiatric or neurological disorders in which anhedonia or motivation-related dysfunction exists (such as major depressive disorder, bipolar I or II disorder, post-traumatic stress disorder, addiction, anhedonia or motivation-related aspects of schizophrenia (e.g. negative symptoms) and Parkinson's disease (e.g. non-motor features such as depression and apathy)).