68 results about "Molecular mechanism" patented technology

The invention relates to the technical field of biology, in particular to application of a biological product expressed by an EsPTI15 gene to regulation and control of tiller number. The nucleotide sequence of the EsPTI15 gene is shown as SEQ ID NO: 1, it is found for the first time that the tiller number of EsPTI15 gene overexpressed siberian wildrye is remarkably increased compared with wild siberian wildrye, and the result proves that overexpression of the EsPTI15 gene can positively regulate and control the plant tiller development process. The discovery not only enriches the molecular mechanism theory of plant tillering regulation, but also verifies that the EsPTI15 gene has the application potential of increasing the tillering number of the plant, and provides key theoretical support and gene resources for subsequent technical research and development of introducing the EsPTI15 gene through gene operation, increasing the tillering number of forage grass and further realizing biomass improvement.

The invention discloses an application of a rice OsWRKY44 gene in plant salt tolerance regulation and control. The invention discovers and verifies that OsWRKY44 is a gene for positively regulating and controlling the salt tolerance of rice in the seedling stage, and provides a new target for analyzing a rice salt-tolerant molecular mechanism. The overexpression of the OsWRKY44 can significantly improve the tolerance of the rice to high-concentration NaCl (200mM) treatment in the seedling stage, is suitable for improving the salt tolerance character of the existing rice variety, and has important theoretical and practical significance for cultivating the salt-tolerant plant variety.

The application discloses application of a SdC2'H gene of Saposhnikovia divaricata and a method for increasing coumarin content in Saposhnikovia divaricata, and belongs to the technical field of bio-agriculture. The SdC2'H gene of Saposhnikovia divaricata and the biological material containing the SdC2'H gene are applied to the regulation of coumarin synthesis in Saposhnikovia divaricata. The method for increasing coumarin content in Saposhnikovia divaricata comprises the steps of increasing the expression amount and / or activity of the SdC2'H gene in Saposhnikovia divaricata. The SdC2'H gene is successfully cloned from Saposhnikovia divaricata for the first time, and a gene overexpression vector is constructed. The SdC2'H gene is positively regulated to control the synthesis of coumarin for the first time, and overexpression of the SdC2'H gene can significantly increase the product content. This not only deepens the understanding of the molecular mechanism of related biosynthesis, but also lays an important foundation for efficient production of high-value coumarin by using genetic engineering technology.

This invention discloses a molecular event visualization method and electronic device, proposing the concept of a molecular film. The method acquires the topological structure file of the drug molecule-target protein and the original trajectory file generated after molecular dynamics simulation of the drug molecule-target protein; based on the original trajectory file and topological structure file, it obtains the keyframe sequence of the molecular dynamics simulation of the drug molecule-target protein; it monitors the keyframe sequence to identify key event sequences of the drug molecule-target protein, and annotates the key event sequences to obtain annotated key event sequences; and it renders the annotated key event sequences to visualize and output the generated rendering data as molecular events, significantly improving the efficiency and depth of understanding molecular mechanisms of action in drug discovery and optimization.

This invention provides a chalcone isopentenyltransferase gene. GiPT16 This invention relates to the GiPT16 protein, amplification primer set, and applications, belonging to the field of biogenetics technology. It is based on *Glycyrrhiza inflata* (GiPT16 protein, amplification primer set, and applications). Glycyrrhiza inflata Using whole-genome sequencing data and a reverse genetics strategy, the key aromatic isopentenyltransferase GiPT16, involved in the isopentenylation modification of chalcone active ingredients, was successfully identified and functionally characterized. This enzyme was confirmed to specifically catalyze the biosynthesis of psoralen and glycyrrhizin C using DMAPP as a donor. It is the first chalcone isopentenyltransferase characterized in *Glycyrrhiza inflata*, filling a gap in the study of key enzymes in the chalcone isopentenylation metabolic pathway of this species and providing important evidence for further elucidating the molecular mechanisms of quality formation in *Glycyrrhiza inflata* medicinal materials.

The invention belongs to the technical field of plant genetic engineering and disease-resistant breeding, and particularly relates to a construction method of soybean GmLNK2 gene four mutants and application of the soybean GmLNK2 gene four mutants in soybean disease-resistant breeding, by means of a CRISPR / Cas9 technology, wild soybean WS82 is used as a material, three target spots are designed for GmLNK2a, GmLNK2b, GmLNK2c and GmLNK2d genes of a GmLNK2 gene family, and simultaneous knockout is performed to obtain a mutant plant. Compared with a wild type WS82, the plant height of a mutant plant is obviously reduced, the length of a main root is obviously shortened, and the leaf area of three-out compound leaves is obviously reduced, which indicates that the mutant plant is more sensitive to pathogenic bacteria in a root rot disease nursery, the susceptibility degree is more serious, and growth and development are obviously inhibited, the key effect of the GmLNK2 gene family in soybean root rot resistance is clarified, and the application of the GmLNK2 gene family in soybean root rot resistance is developed. Important gene resources and technical support are provided for soybean disease-resistant molecular mechanism research and disease-resistant variety cultivation.

This invention belongs to the field of microbial fermentation technology and discloses a method and application for improving the production of staminodesmotic acid (Sclerotium sclerotiorum) based on dynamic osmotic pressure regulation. The method uses *Sclerotium sclerotiorum* as the production strain and employs a synergistic strategy of initial culture medium component optimization, precise osmotic pressure regulation during fermentation, and dynamic feeding to enhance synthesis. Utilizing the dual characteristics of sucrose as a carbon source and osmotic pressure regulator, and the osmotic pressure regulating effect of KCl, the osmotic pressure during fermentation is precisely controlled between 1050-1300 mOsmol / kg. This activates the MAPK-CWI signaling pathway and FKs2 gene expression, directionally promoting staminodesmotic acid synthesis and increasing staminodesmotic acid yield. This invention, by optimizing the initial carbon source and inorganic salt concentration, precisely regulating osmotic pressure during fermentation through feeding, and combining molecular mechanism analysis, achieves a significant increase in staminodesmotic acid yield, reduces industrial production costs, and provides technical support for the large-scale industrial production of staminodesmotic acid.

This invention discloses a major gene for controlling cold tolerance in rice. bHLH150 This invention relates to the field of plant genetic engineering technology, specifically the major gene for controlling cold tolerance. bHLH150 The nucleotide sequence is shown in SEQ ID No:1. After CRISPR / Cas9 editing mutations in rice, the transgenic plants exhibited significantly reduced cold tolerance and became cold-sensitive; while those expressing or overexpressing the gene... bHLH150 The rice exhibits a high survival rate at low temperatures, thus improving its cold resistance. This invention relates to the major cold-resistance gene. bHLH150 Research on the molecular mechanisms regulating cold tolerance in rice is of significant theoretical and practical importance for the breeding of new cold-tolerant rice varieties. It also has broad application prospects and a promising market outlook in the agricultural field.

The invention provides application of a rice OsABI3 gene in reduction of rice cadmium accumulation, and relates to the technical field of gene engineering. The nucleotide sequence of the gene is shown as SEQ ID NO.1. The application comprises the following steps: directionally editing the OsABI3 gene by using a CRISPR / Cas9 technology, constructing a recombinant vector, transforming the recombinant vector into a rice callus by using an agrobacterium-mediated method, and screening to obtain a mutant plant. The rice OsABI3 gene is cloned and analyzed for the first time, the nucleotide sequence of the gene is disclosed, a new perspective is provided for illuminating an unknown molecular mechanism of rice for regulating and controlling Cd transportation, and the invention also proves that the OsABI3 gene has the capability of regulating and controlling Cd accumulation of rice; and tissue anatomy and fluorescent staining experiments prove that the OsABI3 has the capability of limiting radial transportation of Cd ions in a root system.

The invention relates to the technical field of gene detection, in particular to a primer group for detecting a beta-GBP gene of apis cerana and application of the primer group, and the primer group comprises an upstream primer CDS-Acbeta-GBP-1-F with a nucleotide sequence as shown in SEQ ID No.1; the nucleotide sequence of the downstream primer CDS-Acbeta-GBP-1-R is as shown in SEQ ID No. 2; the nucleotide sequence of the upstream primer CDS-Acbeta-GBP-2 is as shown in SEQ ID No.3, and the nucleotide sequence of the downstream primer CDS-Acbeta-GBP-2-R is as shown in SEQ ID No.4; the nucleotide sequence of the upstream primer CDS-Acbeta-GBP-3-F is as shown in SEQ ID No.5, and the nucleotide sequence of the downstream primer CDS-Acbeta-GBP-3-R is as shown in SEQ ID No.6. The invention further discloses a kit for detecting the content of the CDS-Acbeta-GBP-3-F. The Ac beta-GBP gene found by the research can be used as a molecular breeding target spot, disease-resistant bee species highly expressing the gene can be screened by detecting the Ac beta-GBP gene through the primer pair provided by the invention, the method for screening the chalkbrood disease-resistant bee species is enriched, and meanwhile, the Ac beta-GBP provides a new view angle and a new target spot for illuminating a chalkbrood disease-resistant molecular mechanism of Chinese bees, so that the application prospect is wide. And a theoretical basis is laid for developing a novel bee disease prevention and control technology based on RNA interference.

The present application relates to the biological field, and particularly to a soybean Phytophthora psFBP21 protein, a psFBP21 gene and biological materials and application thereof.The amino acid sequence of the soybean Phytophthora psFBP21 protein provided in the present application is shown as SEQ ID NO.2; and the nucleotide sequence of the coding gene is shown as SEQ ID NO.1.Through experiments, it is proved that the soybean Phytophthora psFBP21 protein provided in the present application plays an important role in the growth and development of the soybean Phytophthora itself, and specifically, after the protein is deleted, the mycelium growth of the soybean Phytophthora is slowed down, the number of oospores is reduced, the pathogenicity is reduced, and the like.The above conclusion provides a technical basis for exploring the development and pathogenic molecular mechanism of the soybean Phytophthora, and provides a molecular target for the future research and development of a new fungicide.

This invention discloses an oligosaccharide syltransferase STT3 subunit from *Phytophthora sojae*, its encoding gene, and its applications. The protein sequence of the oligosaccharide syltransferase STT3 subunit provided by this invention is shown in Sequence 2; its encoding gene is shown in Sequence 1. Experiments have demonstrated that the protein provided by this invention plays an important role in the growth and development of *Phytophthora sojae*, specifically manifested in the slowed mycelial growth, reduced number of sporangia and zoospores, and decreased pathogenicity of *Phytophthora sojae* after its deletion. These conclusions provide a technical basis for exploring the molecular mechanisms of *Phytophthora sojae* development and pathogenicity, and provide molecular targets for the future development of novel fungicides.

The present application relates to the field of genetic engineering, in particular to the application of ZmHSP22 protein and its coding gene in regulating the low temperature stress tolerance of plants. The nucleotide sequence of ZmHSP22 gene is shown as SEQ ID No. 1; the protein amino acid sequence is shown as SEQ ID No. 3. After knocking out the ZmHSP22 gene, the cold tolerance of the transgenic plants is improved compared with the control group under low temperature stress treatment. The present application verifies that the ZmHSP22 protein has a regulating function on the low temperature stress tolerance of plants (especially corn), and the ZmHSP22 protein and its related biological materials can be applied to the regulation of the cold tolerance of plants, providing excellent candidate gene resources for the cultivation and improvement of new varieties of cold-tolerant plants. The method for breeding cold-tolerant plants provided has strong purpose compared with the traditional breeding method, significantly shortens the period of cold-tolerant breeding, improves the efficiency of cold-tolerant breeding, lays a theoretical foundation for the study of the mechanism of plant response to adverse signals and the molecular mechanism of tolerance to adverse environment, and has important theoretical and practical significance for promoting the process of corn breeding.

The invention discloses a detection marker of a corn plant type regulatory gene and application of the detection marker. Belongs to the technical field of molecular breeding. The corn dwarf mutant d16 is subjected to phenotype identification, genetic analysis and gene positioning, and finally, the ZmD16 gene is found to be a new allele of a known gene Br2. According to the mutant, G > T single base mutation occurs at 3533bp at the downstream of a fifth exon ATG in a coding region of a wild type ZmD16 gene. A KASP molecular marker is designed according to a mutation site. A foundation is laid for deeply analyzing a molecular mechanism of a corn plant type, and a new material is provided for breeding application of density-tolerant varieties.

The invention belongs to the technical field of genetic engineering and plant genetic breeding, and particularly relates to a gene for regulating and controlling the cold tolerance of rice in the seedling stage and application of the gene. In order to excavate the gene for regulating and controlling the cold resistance of the rice, the nucleotide sequence of the rice gene OsCYP75B3 is mutated by virtue of a CRISPR / Cas9 genome targeted modification technology, and an experimental result shows that the cold resistance of the mutated rice in the seedling stage is remarkably reduced, so that the gene OsCYP75B3 is proved to negatively regulate and control the cold resistance of the rice in the seedling stage; the functional gene for regulating and controlling the cold resistance of the rice in the seedling stage is determined for the first time, and an important reference is provided for analyzing a molecular mechanism of the cold resistance of the rice in the seedling stage; based on the discovery, it is expected that the expression level of the gene OsCYP75B3 is improved through an overexpression technology, the gene OsCYP75B3 is applied to rice genetic engineering breeding, the cold resistance of rice is finally enhanced, and a key technical support is provided for guaranteeing grain safety.

The application provides a non-steady-state wall-free material encapsulation method of food flavor substances, relates to the technical field of chemical informatics, and comprises the following steps: constructing a cavity space size and pocket active group database and a flavor molecule fugacity database for food, wherein the flavor molecule fugacity database comprises the fugacity of food flavor molecules under different environmental conditions; determining a molecular anchor of the active group interaction force between pocket flavor molecules, and matching the cavity space size and pocket active group database with the flavor molecule fugacity database to obtain a mapping relationship between a food matrix and flavor molecules; and under the mapping relationship, outputting a wall-free material encapsulation scheme of food flavor substances according to the molecular anchor. The application realizes "retention-release" non-steady-state balance, achieves the fugacity encapsulation control of flavor substances under the wall-free material condition, can accurately control the mechanism and environmental parameters of flavor molecules, and solves the problems in the flavor steady-state method in the prior art.

This invention relates to the fields of genetic engineering breeding and molecular breeding, and discloses a novel gene for resistance to ear rot. ZmER5 This invention relates to the gene and its application in improving resistance to maize ear rot. The nucleotide sequence of this gene is shown in SEQ ID No. 1, and the amino acid sequence of the encoded protein is shown in SEQ ID No. 2. Through genome-wide association analysis, this invention identified a gene encoding the NAC transcription factor that is significantly associated with resistance to maize ear rot. ZmER5 Using CRISPR / Cas9 gene editing technology, two different homozygous editing types were created. size5 Knockout mutant ,size5 The mutant showed significantly reduced resistance to ear rot, indicating ZmER5 Genes positively regulate maize ear rot resistance. This invention lays the theoretical foundation for elucidating the molecular mechanism of maize ear rot resistance, and also provides new gene resources for breeding superior new maize germplasm with ear rot resistance characteristics.

The application provides a preparation method of a Rhodobacter sphaeroides CsCobT and CsCobN protein complex in vitro, and steps are as follows: constructing an expression vector pET-28a(+)‑Cs CobN , pET-28a(+)‑SUMO‑Cs CobT ; expressing and purifying His-CsCobN protein; preparing a non-tagged CsCobT protein; mixing the purified His-CsCobN protein and the non-tagged CsCobT protein to form a protein complex; separating the complex through affinity chromatography; and refining the complex through gel filtration chromatography. The application adopts a strategy of double vectors, step-by-step purification, in-vitro assembly and multi-dimensional verification, can obtain a CsCobT and CsCobN protein complex with high purity and uniform conformation, and the experimental process can be accurately controlled, the result is specific, and provides high-quality protein materials for subsequent molecular mechanism research and application development of cobalt-chelating enzyme.

The invention discloses application of crassostrea gigas miR-182-5p in negative regulation of melanin synthesis, and belongs to the technical field of aquatic genetic breeding and biology. The miR-182-5p in the crassostrea gigas is interfered by the miR-182-5p inhibitor, synthesis of melanin of the crassostrea gigas is remarkably enhanced after the miR-182-5p is interfered, and the color of the crassostrea gigas is black. In addition, the invention also synthesizes an exclusive miR-182-5p stimulant to treat the crassostrea gigas. Results show that compared with wild crassostrea gigas, the content of melanin in the crassostrea gigas injected with the simulant is lower, and the shell color is white. Therefore, the miR-182-5p is in negative correlation with the synthesis of the melanin of the crassostrea gigas, so that the miR-182-5p not only can be used for selecting or predicting the shell color character of the crassostrea gigas, but also can be used for assisting in screening or culturing varieties with high melanin content by silencing or knocking out the miR-182-5p in the crassostrea gigas. The important effect of miR-182-5p in oyster melanin synthesis is clarified for the first time, the molecular mechanism of mollusk melanin formation can be deeply understood, and the miR-182-5p has important significance in promoting practical application of crassostrea gigas quality breeding.

The invention relates to the technical field of biology, in particular to a p53 protein targeting inhibitor and a design method thereof. The molecular mechanism and microscopic details of interaction of azurin-p53 protein complexes are analyzed through an MD simulation technology and an MM-PBSA free energy decomposition method, key binding sites between the complexes and spatial distribution of the key binding sites are determined, and an affinity model is established, so that a candidate affinity ligand peptide library is constructed. Then, screening and verifying the affinity ligand library through methods of molecular docking, conformation analysis, binding site comparison, hydrophobicity analysis, MD simulation and the like to obtain a high-affinity ligand aiming at a p53 protein target spot, and verifying the binding performance of the affinity peptide ligand and the p53 protein through a double-antibody sandwich ELISA (Enzyme-Linked Immunosorbent Assay) experiment. And screening to obtain the p53 protein inhibitory peptide with high affinity with the p53 protein.

This invention belongs to the field of plant genetic engineering and relates to the application and method of reducing the expression of the Zm7649 gene in improving plant stress resistance. This invention utilizes an EMS mutant with premature termination of expression. Zm7649-ems Heterologous overexpression with Arabidopsis thaliana Zm7649-OE Verified Zm7649 The function of negatively regulating salt stress and high temperature stress was studied, and its preliminary analysis was performed. Zm00001d027649 The molecular mechanism regulating stress involves the interaction between Zm7649 and a maize catalase (Zm4848), with ubiquitination degrading Zm4848 and reducing its protein stability. This invention provides new genetic resources and theoretical basis for maize stress-resistance breeding or germplasm innovation.

The application belongs to the field of plant genetic engineering, and relates to a BrSTM16 gene and application. The nucleotide sequence of the BrSTM16 gene is shown in SEQ ID NO. 1. The application provides the BrSTM16 gene and application which can solve the problem of the molecular mechanism of the response of the white cabbage type winter rape to low-temperature stress and can cultivate new cold-resistant varieties (lines).

The application discloses a Phaseolus vulgaris AURK and application of the interacting protein genes in plant salt tolerance regulation. AURK The nucleotide sequence of the Phaseolus vulgaris AURK gene is shown as SEQ ID NO:1; the Phaseolus vulgaris GRF2 interacting protein gene is GRF5 The nucleotide sequences of the GRF2 gene and the GRF5 gene are shown as SEQ ID NO:2 and SEQ ID NO:3 respectively. The application clarifies the molecular mechanism of AURK-GRF2 and AURK-GRF5 modules in salt stress response through protein interaction verification and salt tolerance identification, provides theoretical support and gene resources for breeding of Phaseolus vulgaris salt-tolerant varieties, and provides a theoretical basis for crop salt tolerance improvement.

The invention discloses a nano antibody interacting with SUMO or bdSUMO tag protein and application of the nano antibody. The nano antibody is S1D9-Nb, and the amino acid sequence is shown in SEQ ID NO.1. The nano antibody S1D9-Nb can be efficiently expressed in escherichia coli, the production process is simple, the cost is low, and the yield is high. The S1D9-Nb has high affinity with the SUMO protein and the bdSUMO protein, and can be used for detecting, enriching and purifying the SUMO and the bdSUMO or the fusion protein of the SUMO and the bdSUMO. In addition, the invention also provides an S1D9-Nb-SUMO compound and a protein crystal and a structure of the S1D9-Nb-bdSUMO compound, the S1D9-Nb-SUMO obtains a high-resolution structure with the resolution of 2.10, and the S1D9-Nb-bdSUMO obtains a high-resolution structure with the resolution of 1.85, so that an important structural basis is provided for understanding a molecular mechanism of SUMO / bdSUMO modification, and the S1D9-Nb-SUMO compound has good application prospects. The method has wide application prospects in the fields of protein engineering, drug research and development, biosensors and the like.

The invention discloses a corn bract character regulation gene ZmE3 and application thereof, and relates to the technical field of molecular biology and genetic engineering. The invention successfully clones and verifies the function of a corn bract character key regulatory gene ZmE3. By creating an overexpression strain and a knockout strain of the transgenic maize, the invention finds that the expression level of the ZmE3 gene is in significant positive correlation with the width, length, thickness and dry weight of bracts. Specifically, the gene is knocked out, so that the bracts are obviously narrowed, shortened and thinned, and the biomass accumulation is reduced; overexpression of the gene produces an opposite gain effect. More importantly, the gene indirectly affects the dehydration process of corn kernels by regulating and controlling the physical characters of bracts. The ZmE3 gene provides a new perspective for analyzing a bract development molecular mechanism, and provides an important technical tool and theoretical support for corn molecular breeding meeting diversified production targets.

The invention discloses a Phmul.002G015100 gene for improving saline-alkaline resistance of kidney beans and application of the Phmul.002G015100 gene, and relates to the technical field of biology. The saline-alkaline tolerance key gene Phmul.002G015100 is successfully excavated from kidney beans through a transcriptome sequencing technology, and transgenic yeast verification experiments prove that the gene can remarkably improve the saline-alkaline tolerance of receptor organisms. In a mixed saline-alkali stress culture medium, the growth state of recombinant yeast into which the Phmul.002G015100 gene is introduced is obviously superior to that of control yeast of an empty vector, which indicates that the gene can effectively enhance the tolerance of organisms to saline-alkali stress. The discovery of the gene not only enriches a plant saline-alkaline tolerance gene resource library, provides a key target for analyzing a saline-alkaline tolerance molecular mechanism of the kidney beans, but also provides a core functional gene for saline-alkaline tolerance genetic improvement of the kidney beans.

This invention relates to a gene that regulates the size of maize kernels. ZmABCB4 Its molecular identification methods and applications belong to the technical field of plant genetics and breeding and functional gene research. ZmABCB4 The gene encodes a member of the ABC transporter B subfamily within the ATP-binding cassette transporter superfamily. Mutations in this gene result in significantly smaller maize kernels. Gene editing of this gene can create small-kernel maize materials with reduced kernel length and width. Based on the sequence differences between this mutant gene and the wild-type gene at the DNA level, a function-specific molecular marker, ZmABCB4-Indel, has been developed for the rapid identification and screening of small-kernel maize materials. This invention provides a theoretical basis and application tools for elucidating the molecular mechanisms of maize kernel size, conducting marker-assisted breeding, and selecting high-quality hybrids, offering important genetic resources and technical support for high-yield and high-quality molecular breeding of maize.

This invention relates to the field of genetic engineering technology, and more particularly to an effective method for inhibiting yak... BMP4 Gene expression shRNA and its application. The shRNA is any one of the following (1) to (3): BMP4-sh1, whose nucleotide sequence is shown in SEQ ID NO:1; BMP4-sh2, whose nucleotide sequence is shown in SEQ ID NO:2; BMP4-sh3, whose nucleotide sequence is shown in SEQ ID NO:3. Experimental data show that BMP4 The interference efficiency of -sh1 is as high as 66.09%, and this efficient silencing effect provides a basis for further research. BMP4 This technology provides a reliable functional tool for understanding the molecular mechanisms involved in hair follicle development and hair cycle regulation. Furthermore, it is simple to operate, has a short cycle time, and can rapidly and specifically reduce [the condition] at the cellular level. BMP4 The expression level laid a key technical foundation for analyzing the genetic regulatory network of wool production traits in yaks.

The application discloses an oligosaccharyltransferase STT3A (staurosporine and temperature-sensitive A) subunit from Phytophthora capsici and a coding gene and application thereof. The oligosaccharyltransferase STT3A subunit provided by the application is shown as sequence 2; and the coding gene is shown as sequence 1. Experiments prove that the protein provided by the application plays an important role in the growth and development of Phytophthora capsici, and the specific performance is that after the protein is deleted, the mycelium growth rate of Phytophthora capsici is slowed down, the zoospore quantity is reduced, and the pathogenicity is reduced, etc. The above conclusion provides a technical basis for exploring the development and pathogenic molecular mechanism of Phytophthora capsici, and provides a potential molecular target for the research and development of a new type of bactericide in the future.

The application relates to the technical field of biology, and particularly relates to a kind of N 6 ,N 6 - Dimethyladenosine and its application. (1) The application locks the key metabolite N 6 ,N 6 - Dimethyladenosine with significant anti-GCRV activity through metabolomics. Experiments prove that the metabolite not only significantly reduces virus load at the in vivo level, but also extremely significantly down-regulates the expression of VP7 at the cell level, thereby inhibiting the assembly and replication of GCRV from the source, and having a clear anti-virus molecular mechanism. (2) The application discloses a new biological function of N 6 ,N 6 - Dimethyladenosine in the field of aquatic anti-virus, and proves that the metabolite can be used as an efficient anti-virus active molecule to inhibit GCRV replication. The application provides important theoretical support and factual basis for developing new, green and safe anti-grass carp hemorrhagic disease drugs and feed additives.