992 results about "Liver tissue" patented technology

Engineered, living, three-dimensional liver tissue constructs comprising: one or more layers, wherein each layer contains one or more liver cell types, the one or more layers cohered to form a living, three-dimensional liver tissue construct. In some embodiments, the constructs are characterized by having at least one of: at least one layer comprising a plurality of cell types, the cell types spatially arranged relative to each other to create a planar geometry; and a plurality of layers, at least one layer compositionally or architecturally distinct from at least one other layer to create a laminar geometry. Also disclosed are arrays and methods of making the same. Also disclosed are engineered, living, three-dimensional liver tissue constructs for use in the augmentation or restoration of one or more liver functions, by in vivo delivery of tissue or utilization of tissue in an extracorporeal device.

This invention discloses isolated short peptides comprising the amino acid sequence Cys-Glu-Phe-His (CEFH) and analogs thereof as well as compositions comprising CEFH peptides and analogs thereof. The CEFH peptides disclosed herein are effective in mediating the denitration of 3-nitrotyrosines (3-NT) in cellular proteins thereby preventing tissue damage associated with excess nitric oxide (NO) and its reactive species. The CEFH peptides disclosed herein are useful in the treatment of ischemia/reperfusion (I/R) injury of various tissues (e.g., I/R injury of heart muscle associated with heart attack or cardiac surgery, I/R injury of brain tissue associated with stroke, I/R injury of liver tissue, skeletal muscles, etc.), septic shock, anaphylactic shock, neurodegenerative diseases (e.g., Alzheimer's and Parkinson's diseases), neuronal injury, atherosclerosis, diabetes, multiple sclerosis, autoimmune uveitis, pulmonary fibrosis, oobliterative bronchiolitis, bronchopulmonary dysplasia (BPD), amyotrophic lateral sclerosis (ALS), sepsis, inflammatory bowel disease, arthritis, allograft rejection, autoimmune myocarditis, myocardial inflammation, pulmonary granulomatous inflammation, influenza- or HSV-induced pneumonia, chronic cerebral vasospasm, allergic encephalomyelitis, central nervous system (CNS) inflammation, Heliobacterium pylori gastritis, necrotizing entrerocolitis, celliac disease, peritonitis, early prosthesis failure, inclusion body myositis, preeclamptic pregnancies, skin lesions with anaphylactoid purpura, nephrosclerosis, ileitis, leishmaniasis, cancer, and related disorders.

The present invention relates to the elucidation of a gene that can act as a novel marker for liver cancer diagnosis and to diagnostic and prognostic measurements of liver cancer using the same. More specifically, it relates to a diagnosis kit that enables diagnostic and prognostic measurement of a liver cancer using a preparation that measures expression levels of at least one gene selected from a group of liver cancer diagnosis markers consisting of S100P, NK4, CCL20, CSPG2, PLAU, MMP12, ESM-1, ABHD7, HCAPG, CXCL-3, Col5A2, MAGEA, GSN, CDC2, CST1, MELK, ATAD2, FAP and MSN and/or a method for diagnostic and prognostic measurement of liver cancer using the same. These have been discovered using normal liver tissues and liver cancer tissues collected from the same liver cancer patient of the present invention and represent the markers whose accuracy and reliability have been greatly improved as markers of liver cancer. The markers of the present invention can be used for the accurate diagnosis and prognosis of liver cancer.

The invention discloses a method for regulating expression of an individual CD2AP down. The method comprises the following step of applying CD2AP down-regulation compatibility to the individual, wherein the CD2AP down-regulation compatibility works through siRNA/shRNA, Crispr/cas9, crispr/cpf1, Talen or ZFNs, and therefore, the expression of the CD2AP in a liver tissue of the individual is regulated down.

A novel strain of lactic acid bacteria was found to be heat resistant and able to grow in a sulfur-limiting medium (SLM) containing a high concentration of sodium selenite. The microorganism is a non-spore forming and Gram-positive coccus, which is identified with >90% confidence using the API biochemical and sugar fermentation tests, ribotyoing and 16S rRNA sequencing as Pediococcus pentosaceus SP80. In the current study, P. pentosaceus SP80 grown on SLM containing 250 ppm sodium selenite produced both organic and inorganic forms of selenium. These selenium compounds can be separated using an anion exchange chromatography technique. The concentrations of selenium detected in the organic and inorganic fractions were 4.34 and 21.7 ppm, respectively. Selenium-enriched bacteria are useful as a source of selenium for supplementing the diets of animals and humans. Animals fed efficacious amounts of the selenium-enriched bacteria show improved feed conversion rates and higher levels of glutathione peroxidase (GPx) activity in heart, kidney and liver tissues indicating an increased absorption and retention of selenium over control diets.

The invention relates to magnetic resonance elastography detection system and method thereof. The detection system comprises a high-field nuclear magnetic resonance whole body imaging system which can carry out liver imaging, and a shear wave excitation device. The detection method comprises the following steps of: (1) inner particle displacement on a phantom or a detected part; (2) magnetic resonance elastography forming; and (3) fibration classification. For preliminary experiments on the phantom as well as studies on background phase noise and experiment researches on the relationship between shear wave transmission and shear wave frequency, the invention has good image quality and obvious effect. The invention establishes a foundation for subsequent MRE researches on the liver tissue, and provides directions and references for elastic researches on other parts.

A tissue graft composition comprising liver basement membrane and a method of preparation of this tissue graft composition are described. The graft composition can be implanted to replace or induce the repair of damaged or diseased tissues.

The invention relates to an image processing technology and particularly relates to an interactive liver tumor region segmentation method based on watershed transform and classification through a support vector machine. The method comprises the following steps: 1) performing segmentation pretreatment on a liver tumor region; 2) performing watershed transform on an image of the pretreated liver region which is obtained in the step 1) and dividing the image of the pretreated liver region into numerous reception basins; 3) calculating four-dimensional characteristic vectors of all the reception basins which are generated by the watershed transform, marking tumor and normal liver tissues in the image of the liver region in an interactive manner and adopting a support vector machine process to classify the reception basins in a characteristic space; and 4) adopting communicating region detection to eliminate a false positive tumor region generated by the classification, and applying morphological operation to fill voids and smoothen edges. The region class is classified, and user marks are further utilized for training parameters of a classifier, thereby effectively improving the segmentation speed and the precision. The method has important application value in the fields of liver surgical planning and the like.

The invention discloses a new method for screening an anti-liver injury medicament by using a model organism zebra fish. The method comprises the following steps: continuously molding the zebra fish serving as a molding object for 3 days by using preferred carbon tetrachloride at the concentration of 1mmol/L to obtain the zebra fish of a liver injury model, and then evaluating the anti-liver injury activity of different receptor medicaments by using ALT and AST levels in the liver tissue of the zebra fish and the pathological change condition of the liver tissue of the zebra fish. Experimental results show that the new method for screening the anti-liver injury medicament by using the model organism zebra fish has high molding success rate, can simulate an in vivo environment to perform quick screening, and has the advantages of strong operability, accurate experimental results, little amount of required reacceptance medicament, strong repeatability, low cost, wide application range, capability of performing batch screening experiments and high work efficiency.

The invention is applicable to the field of medical image processing, and provides a processing method and a processing system for dividing liver graphs of a CT (computed tomography) image. The processing method includes steps of acquiring the to-be-divided image; automatically positioning a liver position in the to-be-divided image by preliminarily testing a liver grayscale volume and liver grayscale; registering and extracting initial liver tissue graphs by rotary registering and sampling bands B with low degrees of freedom; combining distance graphs with the initial extracted liver tissue graphs to detect whether the graphs contain liver tumor tissue graphs or not; and precisely dividing the liver graphs in a registering manner by the aid of a liver distribution probability map and the sampling bands B based on mutual information. The processing method and the processing system have the advantages that manual intervention is omitted in an integral dividing procedure, the average volumetric error of the divided liver graphs is about 7.0%, accurate data can be provided for three-dimensional reconstruction and final simulation surgery for livers of patients, and the processing method and the processing system have certain clinical application value.

The invention relates to a three-dimensional tissue culture device and a three-dimensional tissue culture method. The bioreactor comprises an in-vitro perfusion circulating system, a negative pressure feedback regulation system, a perfusion flow control module and a liver tissue culture module, wherein the in-vitro perfusion circulating system comprises a closed pipeline system, a tissue perfusion generator and a perfusate suspension update device; the negative pressure feedback regulation system comprises an internal pipeline pressure monitoring system, a computer control module and a perfusion device controlled and regulated by a computer; and the liver tissue culture module comprises a constructed sterile culture room and a constructed three-dimensional tissue culture scaffold. The in-vivo environment can be simulated, a device is provided for culturing tissue engineering organs in vitro, and a tool is provided for research of stem cell differentiation and cell growth under three-dimensional conditions.

The invention discloses a liver pathological image segmentation model establishment and segmentation method based on an attention mechanism. The method comprises the steps: firstly carrying out the cutting of a liver tissue pathological section image and a corresponding expert labeling mask image, and obtaining a section image block and a mask image block; then constructing a liver tissue pathological image segmentation network based on multi-scale features and an attention mechanism; and taking the slice image blocks and the mask image blocks as inputs of a segmentation network, taking the obtained segmentation probability graph as an output of the segmentation network, and training the obtained segmentation network to obtain a trained segmentation model. And inputting the liver pathological image to be processed into the segmentation model to obtain a segmentation result. According to the segmentation network, a feature attention mechanism is introduced, attention modeling is carriedout on the position and the channel dimension respectively, the distinguishing capacity of the model for a normal tissue area, an abnormal tissue area and a background is improved, and the influenceof many liver tissue pathological image cavities on model learning is relieved.

The present invention relates to a novel tissue culture system that provides for the long term culture of proliferating hepatocytes that retain hepatic function. Disclosed are methods and compositions for ex vivo culturing of hepatocytes and nonparenchymal cells on a matrix coated with a molecule that promotes cell adhesion, proliferation or survival, in the presence of growth factors, resulting in a long-term culture of proliferating hepatocytes that retain hepatic function. The co-culturing method results in the formation of matrix/hepatic cell clusters that may be mixed with a second structured or scaffold matrix that provides a three-dimensional structural support to form structures analogous to liver tissue counterparts. The hepatic cell culture system can be used to form bio-artificial livers through which a subjects blood is perfused. Alternatively, the novel hepatic cell culture system may be implanted into the body of a recipient host having a hepatic disorder. Such hepatic disorders, include, for example, cirrhosis of the liver, induced hepatitis, chronic hepatitis, primary sclerosing cholangitis and alpha1 antitrypsin deficiency.

Disclosed is a method for detecting CYP450 enzymatic activity by means of micro-liver tissue incubation in vitro, which is characterized in that: a liver trace puncturing method used in clinic takes micro-liver tissues of 0.1 to 0.2g, or kills a mouse to take out the liver in an animal experiment, and builds a micro-liver tissue in vitro incubation system, then uses a one-probe medicine to perform the liquid phase chromatography tandem mass spectrometry for detecting probe substrates and concentration of corresponding metabolites, and obtains the activity of P450 enzyme according to metabolite ratios thereof. The method has the advantages of: 1. micro scale: only micro-liver tissues of 0.1 to 0.2g are needed for detecting cytochrome P450enzyme activity, and can be used for animal experiment and checking clinic micro-liver tissue liver drug enzyme activity; 2. time saving and convenience: costly ultracentrifugation equipment used in the conventional method is saved, costs and time for detection are greatly saved, and liver enzyme metabolic condition simulation is better; and 3. establishing indexes for evaluating the enzyme metabolic activity: metabolite ratios.

The invention discloses a combination marker used for liver cancer detection and application thereof. The combination marker used for liver cancer detection comprises the respective methylation levelsof adenylate kinase 055957 (AK055957), 3-hydroxybutyrate dehydrogenase 1 (BDH1), chromosome 6 open reading frame 223 (C6orf223), disabled homolog 2-interacting protein (DAB2IP), human coagulation factor XII (F12), Golgi reassembly stacking protein (GRASP), leucine-rich repeat-containing protein 4 (LRRC4), nuclear factor I/X (NFIX), 5-oxoprolinase (ATP-hydrolysing) (OPLAH), recombinant protein tyrosine phosphatase F interacting protein 1 (PPFIA1), Pleckstrin homology and SEC7 domain-containing protein 4 (PSD4) and T-box 15 (TBX15). Experiments prove that whether the genome DNA or plasma circulating free DNA (cfDNA) of to-be-detected liver tissue comes from patients with liver cancer or patients without liver cancer can be judged by detecting the combination marker, and accuracy is relatively high; and the combination marker has significant application value.

The present invention provides a process for preparing a transplantable liver tissue, cell colonies suitable for the process, and a process for preparing them. At least about 70 % of the cells in the small hepatocyte-rich colonies of the present invention are small hepatocytes. In particular, each colony comprises about 10 to 30 cells and the number of the small hepatocytes is at least about 70 % based on the total number of the cells in the colony. A process for preparing such small hepatocyte-rich colonies is also disclosed. The present invention also discloses a process for inducing the maturation of the small hepatocyte-rich colonies into a liver tissue and a method of estimating the effect of a drug, particularly the effect thereof connected with normal liver function in vitro, using the cell colonies enriched with the small hepatocytes which have been induced to be matured.

The invention provides an artificial liver tissue and a preparation method thereof. The artificial liver tissue comprises a lower chip, a first microporous membrane, a middle chip, a second microporous membrane and an upper chip from the top to the bottom in sequence in a laminated manner. Three layers of cavities of a lower cavity, a middle cavity and an upper cavity are formed in the lower chip, middle chip and upper chip respectively. Three cavities are all grouted with culture solution containing growth factor, extracellular matrix containing hepatic parenchymal cells and suspension liquid containing endothelial cells. Adjacent cavities are separated by the microporous membrane. The microporous membrane can separate the matrix flow outside of the cell, and cause no influence to the cell migration. The culture solution and is fed through an inlet and an outlet of the upper cavity and lower cavity. Under the guidance of the growth factor, endothelial cells pass through the extracellular matrix of the middle cavity to form a lumen, and form the artificial liver tissue containing capillaries. The artificial liver tissue can simulate the human body liver tissue in a good way, and simulate the intrahepatic capillary functions of mass transfer, selective permeability, toxic reaction and the like.

According to the liver tumor automatic classification method and device based on multi-stage CT image analysis, full-automatic bile duct cell carcinoma and hepatocellular carcinoma can be recognized,and a high-precision bile duct cell carcinoma and hepatocellular carcinoma recognition model is obtained. The method comprises the following steps: (1) acquiring a contrast-enhanced abdominal CT scanning image, storing the contrast-enhanced abdominal CT scanning image as an arterial phase, a portal vein phase and a delay phase, and carrying out definite diagnosis on liver cancer categories to which all data belong to serve as a model training gold standard; (2) constructing a three-dimensional full convolutional neural network segmentation model, and segmenting the intrinsic characteristics ofthe liver tissue in each stage from the abdominal CT image through model training learning; (3) constructing a three-dimensional convolutional neural network classification model; and inputting the image data obtained by segmentation into a classification model for training, so as to enable the model to perform joint learning and training on the cancer features in multiple periods, thereby predicting the category to which the cancer belongs, comparing the prediction result with a gold standard, and supervising the training process of the model in a loss value feedback mode.

The invention relates to the technical field of biomedical engineering and particularly relates to a long-term in-vitro culture and directional differentiation system and method for a liver stem cell. The long-term in-vitro culture and directional differentiation system comprises an amplification culture medium with specific chemical components, and a differential medium with specific chemical components, wherein the amplification culture medium is used for carrying out in-vitro culture of a mouse or human liver stem cell, and the differential medium is used for carrying out induced differentiation on the mouse or human liver stem cell to form a matured liver cell. By using the long-term in-vitro culture and directional differentiation system and method, a selectively-amplified liver stem cell in mother cells of the liver can be obtained from a mouse embryonic liver tissue or through human multipotential stem cell differentiation, the liver stem cell can be cultured for more than 20 generations under such a condition, and the stable molecular phenotype of the liver stem cell is maintained. By using the long-term in-vitro culture and directional differentiation system and method, the cultured mouse or human liver stem cell can be further subjected to induced differentiation to form the matured liver cell with functions of secreting albumin, metabolizing urea and the like.

The invention discloses an excision side generating method and device of a liver operation. The method comprises that a 3D reconstruction model is generated according to an image segmentation result;liver segments are divided according to the 3D reconstruction model; whether each liver segment includes a focus is determined; a minimal blood supply area RS,1 and a minimal blood return area RS,2 including the focus are determined; and an excision side of the liver operation is generated on the basis of the RS,1 and RS,2. Registering is carried out; and the 2D reconstruction model is generated according to a liver image, a focus image and a blood vessel image segmentation result. When the focus only exists in one liver segment, the excision side of the liver operation is generated by combining the minimal blood supply area with the minimal blood return area of the liver segment with the focus; and when multiple liver segments include focuses, the excision side of the liver operation is generated according to an area by combining the minimal blood supply areas the minimal blood areas of the liver segments and an excision area. Thus, a doctor is helped make a better operation plan, andmore healthy liver tissues can be reserved.

The present invention is the method of establishing mouse model of viral hepatitis. The method includes selection of mouse hepatitis virus-III (MHV-3) of 100-1000 pFu, injecting infection of variousvariety mice of BALB/CJ, C3H/HEJ and A/J mice, and observing the survival rate and liver tissue change. Via the said process, three kinds of viral hepatitis mouse model are established, of fulminanthepatitis, chronic hepatitis and symptom-free mouse separately. The establishment of the model provides powerful facilities for systematic research of viral hepatitis in genetic regulation, molecular mechanism and prevention and treatment.