23 results about "HEPES" patented technology

The invention belongs to the technical field of biology, and relates to a bronchoalveolar lavage fluid (BALF) preserving fluid, a kit and a cytological test method. The preservation solution is composed of TCEP, N-acetylcysteine, EDTA.2Na, trehalose, Proclin300, methanol, sodium chloride and HEPES, and all the components have a synergistic effect to achieve sample viscosity removal, oxidation resistance, corrosion prevention, cell protection and pH stabilization. The preserving fluid is suitable for morphological observation and nucleic acid detection of BALF cells, and can stably preserve samples at room temperature and maintain completeness of cell membranes and clear morphological structure. The kit comprises two preparations which are mixed to form a working solution, and the working solution can be directly used for sample preservation on a sampling site. The cytological test method comprises the steps of sample collection, preservation, centrifugal slide preparation, Wright-Giemsa staining and microscopic observation. Experimental results show that preservation solutions of different formulas are compatible with a chromosome system, the cell morphology is kept stable within 3 days, the dyeing effect is good, and it is proved that the preservation system can remarkably improve the preservation stability and detection reliability of BALF samples and is suitable for cytology and molecular diagnosis application of lower respiratory diseases.

The present application relates to the technical field of water treatment, and discloses a composite carbon source for removing nitrate in an aquarium system, a preparation method and application thereof. The components of the composite carbon source include glycerol, slow-release carbon source, zeolite, citrate, vitamin, HEPES and binder. The glycerol and slow-release carbon source in the composite carbon source form synergy, the former can quickly start microbial action and rapidly reduce the concentration of nitrate, and the latter provides long-term support to ensure the sustainability of the degradation effect. The combination not only improves the denitrification efficiency, but also avoids the side effects caused by excessive short-term carbon source. In addition, the glycerol and citrate are synergistic in both chemical and biological aspects: the former is a short-term and rapid energy source, and the latter plays a role in metal buffering and environmental regulation, and both provide good support for efficient denitrification of microorganisms. In combination with the physical and chemical properties of other components, the stability and efficiency of the composite carbon source for denitrification are improved.

The invention discloses a blue copper peptide composition for repairing scars and a preparation method of the blue copper peptide composition, and belongs to the technical field of preparation of biological medicine preparations. The composition is prepared from freeze-dried powder of copper blue peptide, polyquaternium-73, asiaticoside, nicotinamide, polydimethylsiloxane, betaine-propylene glycol ionic liquid and a gel matrix. According to the invention, the blue copper peptide is coated in a permeation enhancing system constructed by the betaine-propylene glycol ionic liquid and the HEPES, so that the enzymolysis loss of the blue copper peptide is reduced, and the biological activity of the blue copper peptide is maintained; a temperature-sensitive gel matrix is constructed by carboxyl esterified modified sodium alginate and poloxamer, the carboxyl esterified modified sodium alginate is prepared by grafting n-butyl alcohol to a hydrophilic main chain of sodium alginate through a Fischer esterification method, the gel matrix is in a liquid state at normal temperature, after the gel matrix is smeared on a scar part, the gel matrix is converted into semi-solid gel under the influence of body temperature, and slow and continuous release of blue copper peptide can be achieved. The traditional Chinese medicine composition has the effect of repairing scars and is suitable for chronic scars needing long-term nursing.

This invention provides a fluorescence-enhanced composite probe based on gold nanoclusters anchored on a zinc-based coordination polymer, its preparation method, and its application, belonging to the field of probe preparation technology. The method involves first mixing an aqueous solution of glutathione and an aqueous solution of tetrachlorohydrin to obtain a gold nanocluster solution; then adding adenine and water to a 4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid buffer solution and stirring until homogeneous, followed by the addition of a zinc chloride solution to obtain a zinc-based coordination polymer; finally, the gold nanocluster solution and the zinc-based coordination polymer solution are mixed homogeneously to obtain the fluorescence-enhanced composite probe based on gold nanoclusters anchored on the zinc-based coordination polymer. The probe of this invention can rapidly and sensitively recognize adenosine triphosphate (ATP). It can also be used to construct fluorescent hydrogels by mixing this material with κ-carrageenan; the hydrogels exhibit a sensitive response to ATP and good self-healing ability.

The invention discloses a preparation method of a ferritin-based magnetic resonance nanoprobe targeting hepatic stellate cells, which comprises the following steps: S1, preparing an HEPES salt solution with the working concentration of 10 mmoL / L, and adjusting the pH value to 8.3 by using a NaOH solution; s2, potassium chloroplatinite powder is dissolved in the HEPES salt solution in the S1, a ferritin solution is added, then the mixture is placed on a magnetic stirrer to be stirred for 1 h at the room temperature, and standing is conducted for 5 h. According to the preparation method of the hepatostellate cell targeting ferritin-based magnetic resonance nanoprobe, the ferritin-based magnetic resonance nanoprobe is endowed with good MRI T1 imaging ability after loading of metal manganese ions and platinum, and non-specific recognition of a ferritin receptor in a liver is shielded by modifying excessive double-active ester polyethylene glycol; then, through mediation of the specific targeting molecule RGD peptide, the nanoprobe is efficiently delivered to the fibrosis liver, the interception effect of normal liver tissue is avoided, the imaging effect is greatly enhanced, and imaging diagnosis of mouse hepatic fibrosis can be achieved.

The application discloses a [2.2] cyclophane coumarin fluorescent probe, a preparation method and a method for detecting chiral molecules, and the fluorescent probe comprises a fluorescent probe structure, and the fluorescent probe structure is shown as formula (I). The [2.2] cyclophane skeleton is introduced into the coumarin molecule, and the fluorescent probe can be applied to fluorescence recognition of chiral molecules. The fluorescent probe can chemoselectively and enantioselectively recognize histidine in a mixed solution of tetrahydrofuran and HEPES aqueous solution, and can quantitatively detect the concentration of histidine and the ee value of histidine. The probe is an achiral fluorescent probe, can reduce the difficulty of separation of chiral compounds, greatly reduces the synthesis cost of the fluorescent probe, and opens up a new amino acid recognition probe.

This invention discloses a method for screening catalysts for the oxygen evolution reaction based on electrochemiluminescence quenching. The catalyst to be tested is loaded onto the working electrode, and then Ru(bpy)3 is added. 2+ In the electrolyte of / Hepes, applying an anodic potential to excite ECL accelerates the OER to generate O2, and O2 quenches the ECL signal. The signal intensity is negatively correlated with the catalytic activity. This method can be used for parallel detection on a single-electrode imaging array, achieving high throughput, visualization, and quantitative screening. It is applicable to various OER catalysts such as polyoxometalates and oxides, and has the advantages of being fast, low-cost, and easy to operate.

This invention discloses a peroxynitroso anion fluorescent probe with AIE effect, its synthesis method, and its applications. The near-infrared fluorescent probe TPS was designed and synthesized in this invention. It is nearly colorless under ultraviolet light irradiation but emits bright yellow fluorescence upon reaction with peroxynitroso anions. Detection showed that TPS reacts with ONOO in a HEPES:acetonitrile = 1:1 system. ‑ The response time was 100 min, after adding 120 uL of 0.5×10 ‑3 mol·L ‑1 ONOO ‑ The reaction is complete. TPS exhibits high specificity, reacting only with peroxynitrosoanion among various anions, demonstrating high sensitivity. TPS reacts with ONOO at pH 4-8. ‑ The ion response exhibits good stability, and TPS is effective against ONOO. ‑ The response exhibits good linearity, with a detection limit of 0.08232. µmol·L ‑1 Probe TPS and ONOO ‑ The reaction, after different concentrations of THF, exhibited a typical AIE effect.

A perfusion stock composition, for preserving a donor organ for transplantation, comprising: a source of 60 to 100 mM Na+; a source of 10 to 20 mM K+; a source of 5 to 10 mM Mg2+; a source of 0.25 to 0.75 mM Ca2+; 10 to 40 mM Tris(hydroxymethyl)aminomethane hydrochloride (Tris or THAM), 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), 3-(N-morpholino)propanesulfonic acid (MOPS), 2-(N-morpholino)ethanesulfonic acid (IviES), N,N-bis-(2-hydroxyethyl)-2-aminoethanesulfonic acid (BES), or N / -tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid (TES); a source of 10 to 30 mM HCO3; 1 to 30 mM glucose; 1 to 20 U / L insulin; 1 to 10 mM fructose diphosphate or a salt thereof, 1 to 40 mM aspartate or glutamate; 1 to 10 mM adenosine, cAMP or cGMP; 1 to 10 mM reduced glutathione; and 30 to 100 mM lactobionate or mannitol; and, optionally, a diluent. The present disclosure also provides a perfusion composition, a kit, a method, and a perfusion apparatus, each related to the perfusion stock composition.

The application provides a method for degrading chlorohydrocarbon in water by using illite reinforced ball-milling sulfidation micro zero-valent iron, which comprises the following steps: first, a buffer solution with pH=7 is prepared by using 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, and an oxygen-free aeration treatment is performed; then, illite and S-mZVI bm are mixed to prepare a particle mixture, and the particle mixture is sealed and stored in the buffer solution with pH=7; finally, chlorohydrocarbon pollutants are added into the particle mixture of illite and S-mZVI bm , and a sealed oscillation reaction is performed, so that the concentration of the chlorohydrocarbon in the reaction system is determined. The application directly reinforces the activity of S-mZVI bm material by using illite, which is a clay mineral widely distributed in nature, so that the defects of S-mZVI bm , such as large particle size, slow degradation rate and decreased activity in the later reaction stage, are effectively solved, and the long-term reaction activity of the particles is ensured. The raw materials are green and environmentally friendly, the process is simple, and the materials are easy to obtain.

This invention provides a culture medium for closed-system culture of mesenchymal stem cells, comprising the following components: 1% (v / v) human platelet lysate, 10-15 g / L basal culture medium powder, 15-20 mM HEPES, 1.0-2.0 g / L sodium bicarbonate, and 0.1-1.0 g / L sodium chloride; wherein the basal culture medium powder includes non-essential amino acids, D-glucose, L-glutamine, specific growth factors, inorganic salts, and vitamins. Using the culture medium provided by this invention for mesenchymal stem cell culture eliminates the need for exogenous CO2, reducing equipment costs and process complexity; furthermore, it results in cell viability ≥97%, more concentrated particle size distribution, and more stable expression of surface markers, which is beneficial for large-scale production.

A perfusion stock composition, for preserving a donor organ for transplantation, comprising: a source of 60 to 100 mM Na+; a source of 10 to 20 mM K+; a source of 5 to 10 mM Mg2+; a source of 0.25 to 0.75 mM Ca2+; 10 to 40 mM Tris(hydroxymethyl)aminomethane hydrochloride (Tris or THAM), 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), 3-(N-morpholino)propanesulfonic acid (MOPS), 2-(N-morpholino)ethanesulfonic acid (IviES), N,N-bis-(2-hydroxyethyl)-2-aminoethanesulfonic acid (BES), or N / -tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid (TES); a source of 10 to 30 mM HCO3; 1 to 30 mM glucose; 1 to 20 U / L insulin; 1 to 10 mM fructose diphosphate or a salt thereof; 1 to 40 mM aspartate or glutamate; 1 to 10 mM adenosine, cAMP or cGMP; 1 to 10 mM reduced glutathione; and 30 to 100 mM lactobionate or mannitol; and, optionally, a diluent. The present disclosure also provides a perfusion composition, a kit, a method, and a perfusion apparatus, each related to the perfusion stock composition.

The invention provides an Escherichia coli culture medium capable of efficiently promoting growth and a preparation method thereof, and belongs to the technical field of microbial culture mediums. The Escherichia coli culture medium per liter comprises the following components: 10 to 15 g of tryptone, 5 to 10 g of yeast extract, 2.5 to 3 g of glucose, 3 to 5 mL of glycerin, 4 to 6 g of sodium chloride, 5 to 10 g of dipotassium phosphate trihydrate, 1 to 5 g of potassium dihydrogen phosphate, 0.1 to 1 g of magnesium sulfate heptahydrate, 0.01 to 0.1 g of calcium chloride, 0.1 to 1 g of L-glutamine, 0.01 to 0.02 g of vitamin B group mixture, 0.5 to 2 g of betaine and an HEPES buffer solution, and the final concentration of HEPES is 10 mM. Compared with an LB culture medium, the escherichia coli culture medium has the advantages that the growth rate is increased by 55.6%, the recombinant protein expression quantity is increased by 59.1%, and the plasmid extraction yield is increased by 69.4%.

The invention relates to a method for synthesizing spherical nucleic acid by reducing gold trichloride through alkynyl-modified DNA. The method comprises the following steps: preparing a gold trichloride aqueous solution, an alkynyl-modified DNA solution, a poloxamer 188 aqueous solution and an HEPES solution; adding a gold trichloride aqueous solution, an alkynyl modified DNA solution and a poloxamer 188 aqueous solution into an HEPES solution, and reacting to obtain the spherical nucleic acid of the DNA modified gold nanoparticles. According to the method, the alkynyl-modified DNA is used for reducing gold trichloride to synthesize the spherical nucleic acid in one step for the first time, the use of the alkynyl-modified DNA realizes rapid one-step synthesis of the DNA-modified gold nanoparticles, and the synthesis and modification processes of the spherical nucleic acid are greatly simplified. The preparation method is simplified, and the reaction time is shortened.

The invention belongs to the technical field of biological detection, and particularly relates to a sample treating fluid for detecting bordetella pertussis, a detection method, a product and application. The sample treatment liquid comprises a lysis working solution (WLB) and a post-treatment diluent (PD), the WLB is composed of choline tartrate, betaine, glycerin, TCEP, IGEPAL CA-630, HEPES and non-enzyme deionized water, and the PD is composed of bovine serum albumin and non-enzyme deionized water. The kit is suitable for common clinical samples such as sputum and nasopharynx swab eluent, bordetella pertussis can be efficiently split, target nucleic acid is fully released, and meanwhile matrix inhibitors such as mucoprotein, heme and protease in the samples are effectively removed. The kit is simple and convenient to operate, free of complex purification steps, high in detection sensitivity, good in reproducibility, high in specificity and free of cross reaction, and provides reliable technical support for early precise diagnosis and epidemic prevention and control of pertussis.

The invention belongs to the technical field of cell culture, and particularly relates to a culture method and application of human perinervous cells. The culture method comprises the following steps: inoculating HBVP into a compound basic culture medium which is prepared from DMEM and MCDB 131 mixed in a ratio of 1: 1, accurately adding HEPES with the final concentration of 10 mM, 1% (v / v) Glutamax, 1% (v / v) N2, 1% (v / v) G-5, 1% (v / v) B-27, 10 ng / mL hEGF, 5% (v / v) fetal calf serum and 1% (v / v) penicillin-streptomycin into the compound basic culture medium to form a special HBVP culture solution, and the culture solution can provide an optimal growth microenvironment for HBVP. The cell culture medium can effectively promote continuous and stable proliferation of cells and maintain high activity, can be used as a high-quality cell source for HBVP related basic research, preclinical research and potential application, and has good practicability and popularization value.

Anti-SIRPa antibody formulation, suitable for parenteral administration, comprising: an anti-SIRPα antibody that inhibits signaling via the SIRPα-CD47 axis at a concentration between approximately 20 mg / ml and approximately 100 mg / ml; a buffer component selected from the group consisting of approximately 20 mM L-histidine and 20 mM sodium phosphate; a disaccharide comprising an α-glycosidic bond and present at a concentration of approximately 8 wt. / vol.%; approximately 0.01 wt% polysorbate 20; and a pH value of approximately 5.7 to approximately 6.3; which is free of glycine, arginine, carbonate, HEPES, citrate and acetate.

The application belongs to the technical field of in vitro diagnostic reagents, and discloses a cleaning solution for acridinium ester chemiluminescence immunoassay of allergens, a preparation method and application thereof. The cleaning solution is composed of HEPES buffer, a triple surfactant system (poloxamer 188, sodium deoxycholate and octyldecyl glucoside), glycerol, PEG8000, disodium EDTA and a composite preservative of Proclin 950 and BHT. The triple surfactant synergistic system is adopted to reduce non-specific adsorption, the HEPES weak alkaline buffer system avoids the inhibition of phosphates on acridinium ester luminescence, and the composite preservative system solves the toxicity and stability problems of traditional preservatives. The cleaning solution can significantly reduce the background luminescence value, the inhibition rate of hemolysis / lipemia / jaundice interference is less than 5%, the detection accuracy deviation is within ±5% (r>0.99), the precision is less than 3.5%, and the room temperature stability is more than 13 months, and the comprehensive performance is better than that of the commercially available products.

The invention discloses a thromboelastogram blood coagulation activation detection reagent as well as a preparation method and application thereof. The thromboelastogram blood coagulation activation detection reagent comprises an endogenous blood coagulation activator and a composite solvent, the endogenous blood coagulation activator is selected from any one or more of ellagic acid, an ellagic acid hydrate, ellagic acid salt and a metal complex of the ellagic acid; the composite solvent comprises an organic solvent and an aqueous solvent, and the organic solvent comprises dihydrolevoglucose ketone (Cyreene) and one or more of gamma-valerolactone (GVL) and N, N-diethyl formamide (DEF); the aqueous solvent is selected from one of water, normal saline and a buffer solution, and the buffer solution is selected from any one or more of HEPES, TAPS, MOPS, BES, TES and Tris buffer solutions.

The invention belongs to the technical field of nucleic acid detection, and particularly relates to a tongue swab sample preserving fluid and application thereof. Comprising 20 mM to 100 mM of Tris-HCl or HEPES, 0.3% to 0.6% (v / v) of Tween 20, 0.04% to 0.08% (v / v) of Triton X-100, 0.03% to 0.05% (w / v) of guanidine isothiocyanate, 0.1 mM to 0.3 mM of EDTA-2Na (Ethylene Diamine Tetraacetic Acid), 0.1 wt% to 0.2 wt% of cane sugar and 20 mM to 25 mM of NaOH. The pH value of the tongue swab sample preserving fluid is 7-8, and the tongue swab sample preserving fluid can adapt to enriched magnetic beads, so that effective extraction of low-abundance mycobacterium tuberculosis nucleic acid in a tongue swab sample is realized.

The invention discloses a method for amplifying Treg cells and a Treg cell culture medium. The method for amplifying the Treg cells comprises the following steps: extracting PBMC (peripheral blood mononuclear cells) from autologous plasma; carrying out magnetic bead sorting on CD4 + T cells in the PBMC; the cell suspension is resuspended in a Treg cell culture medium, culture is conducted, cells are collected after 14 days, and a Treg amplification culture medium comprises 5% thermally inactivated autologous plasma, glutamine, HEPES, beta-ME, Rapamycin, Decitabine, IL-2 and alphaCD3 / CD28. According to the invention, CD4 + T cells can be efficiently induced to generate Treg cells, and the obtained autologous Treg cells can be applied to various autoimmune diseases and other clinical demand conditions for inducing peripheral immune tolerance.