46 results about "HEPES" patented technology

The invention discloses a reagent for reducing the agglomeration rate of suspended HEK293F cells, a culture method and a culture medium. The reagent comprises the following components: 0.01-0.1% (v / v) of a nonionic surfactant, 0.05-0.2% (v / v) of polysaccharide or a derivative thereof, 1-5mM of L-glutamine or a derivative thereof, 0.001-0.01 mM of FeCl3, 14-16mM of HEPES and 50-100mu M of an RGD peptide inhibitor. According to the anti-caking agent, the nonionic surfactant, the polysaccharide or the polysaccharide derivative, the L-glutamine or the L-glutamine derivative, the FeCl3, the HEPES and the RGD peptide inhibitor are optimally proportioned, so that the HEK293F cell caking rate is remarkably reduced to 30% or below, after the anti-caking agent is added, cells are in a uniform single-cell suspension state, macroscopic cell agglomerates do not exist, and the anti-caking effect is good. The uptake efficiency of cells on nutrient substances is obviously improved. Compared with a commercially available PEG (Polyethylene Glycol)-containing anti-caking agent, the anti-caking agent disclosed by the invention has the advantages that the anti-caking rate is lower than 35% (the anti-caking rate is only 30% vs after being cultured for 48 hours, and the anti-caking rate is 40%) while the cell viability is maintained to be 95% or above, and no cytotoxic accumulation exists after long-term passage (more than or equal to 20 generations).

The invention provides an oral thylakoid product for resisting organ failure and aging, which is prepared by the following steps: extracting thylakoid from plants including spinach, arabidopsis thaliana, lettuce, cabbage, green algae or wheat seedlings, redissolving the thylakoid into a buffer salt solution A, and carrying out ultrasonic treatment for 10-20 minutes under the ultrasonic power of 200-500W to prepare a suspension; adjusting the concentration of the suspension to 0.5-2 mg / ml, and obtaining a nano capsule-like body with the particle size of 200-500 nm by using a microfluidic technology; the buffer salt solution A is prepared from 10 to 100 mmol / L of 4-hydroxyethyl piperazine ethanesulfonic acid (HEPES), 1 to 2 mmol / L of ethylenediamine tetraacetic acid (EDTA) disodium, 0.5 to 4 mmol / L of magnesium chloride (MgCl2), 1 to 10 mmol / L of ascorbic acid and 100 to 500 mmol / L of sapoeda sodium (SNAC), and the pH value of the buffer salt solution A is adjusted to 7.5 to 8.0; and the concentration of the capsule in the buffer salt solution A is 100-400 mg / mL. According to the product, self QPRT enzyme of the thylakoid is used for efficiently catalyzing de novo synthesis of NAD + of whole body tissues and organs, so that multiple physiological processes such as energy synthesis enhancement, oxidative stress reduction and inflammatory reaction relief are finally realized, organ failure is efficiently reversed, and organ aging is relieved.

This invention provides a simple synthesis method for Fe-Ag / CMP nanopolymers with catalase-like activity and their application in hydrogen peroxide detection, belonging to the field of bioanalytical technology. The method of this invention includes the following steps: (1) preparing a 4-hydroxyethylpiperazine thiosulfonic acid (HEPES) standard buffer solution; (2) adding cytidine monophosphate (CMP) to the solution obtained in step (1) and stirring until the CMP is fully dissolved. Then, under vigorous stirring, ferric nitrate aqueous solution and silver nitrate aqueous solution are added sequentially, at which point the colorless solution turns pale yellow; (3) transferring the mixed solution obtained in step (2) to a centrifuge tube, centrifuging at 12000 rpm for 8 min to collect the precipitate, washing it three times with distilled water, and finally adding 4 mL of distilled water to obtain a 1 mg / mL Fe-Ag / CMP suspension. This invention utilizes CMP as a biological ligand and Ag... + Fe 3+ Simultaneous coordination, and the reducing amino groups on CMP can directly transfer Ag... + In situ reduction to silver nanoparticles (AgNPs) led to the one-step synthesis of a bimetallic Fe-Ag / CMP nanopolymer with hydrogen peroxide-like nanozyme activity, enabling the detection of hydrogen peroxide.

The invention belongs to the technical field of biology, and relates to a bronchoalveolar lavage fluid (BALF) preserving fluid, a kit and a cytological test method. The preservation solution is composed of TCEP, N-acetylcysteine, EDTA.2Na, trehalose, Proclin300, methanol, sodium chloride and HEPES, and all the components have a synergistic effect to achieve sample viscosity removal, oxidation resistance, corrosion prevention, cell protection and pH stabilization. The preserving fluid is suitable for morphological observation and nucleic acid detection of BALF cells, and can stably preserve samples at room temperature and maintain completeness of cell membranes and clear morphological structure. The kit comprises two preparations which are mixed to form a working solution, and the working solution can be directly used for sample preservation on a sampling site. The cytological test method comprises the steps of sample collection, preservation, centrifugal slide preparation, Wright-Giemsa staining and microscopic observation. Experimental results show that preservation solutions of different formulas are compatible with a chromosome system, the cell morphology is kept stable within 3 days, the dyeing effect is good, and it is proved that the preservation system can remarkably improve the preservation stability and detection reliability of BALF samples and is suitable for cytology and molecular diagnosis application of lower respiratory diseases.

The present application relates to the technical field of water treatment, and discloses a composite carbon source for removing nitrate in an aquarium system, a preparation method and application thereof. The components of the composite carbon source include glycerol, slow-release carbon source, zeolite, citrate, vitamin, HEPES and binder. The glycerol and slow-release carbon source in the composite carbon source form synergy, the former can quickly start microbial action and rapidly reduce the concentration of nitrate, and the latter provides long-term support to ensure the sustainability of the degradation effect. The combination not only improves the denitrification efficiency, but also avoids the side effects caused by excessive short-term carbon source. In addition, the glycerol and citrate are synergistic in both chemical and biological aspects: the former is a short-term and rapid energy source, and the latter plays a role in metal buffering and environmental regulation, and both provide good support for efficient denitrification of microorganisms. In combination with the physical and chemical properties of other components, the stability and efficiency of the composite carbon source for denitrification are improved.

The invention discloses a blue copper peptide composition for repairing scars and a preparation method of the blue copper peptide composition, and belongs to the technical field of preparation of biological medicine preparations. The composition is prepared from freeze-dried powder of copper blue peptide, polyquaternium-73, asiaticoside, nicotinamide, polydimethylsiloxane, betaine-propylene glycol ionic liquid and a gel matrix. According to the invention, the blue copper peptide is coated in a permeation enhancing system constructed by the betaine-propylene glycol ionic liquid and the HEPES, so that the enzymolysis loss of the blue copper peptide is reduced, and the biological activity of the blue copper peptide is maintained; a temperature-sensitive gel matrix is constructed by carboxyl esterified modified sodium alginate and poloxamer, the carboxyl esterified modified sodium alginate is prepared by grafting n-butyl alcohol to a hydrophilic main chain of sodium alginate through a Fischer esterification method, the gel matrix is in a liquid state at normal temperature, after the gel matrix is smeared on a scar part, the gel matrix is converted into semi-solid gel under the influence of body temperature, and slow and continuous release of blue copper peptide can be achieved. The traditional Chinese medicine composition has the effect of repairing scars and is suitable for chronic scars needing long-term nursing.

This invention provides a fluorescence-enhanced composite probe based on gold nanoclusters anchored on a zinc-based coordination polymer, its preparation method, and its application, belonging to the field of probe preparation technology. The method involves first mixing an aqueous solution of glutathione and an aqueous solution of tetrachlorohydrin to obtain a gold nanocluster solution; then adding adenine and water to a 4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid buffer solution and stirring until homogeneous, followed by the addition of a zinc chloride solution to obtain a zinc-based coordination polymer; finally, the gold nanocluster solution and the zinc-based coordination polymer solution are mixed homogeneously to obtain the fluorescence-enhanced composite probe based on gold nanoclusters anchored on the zinc-based coordination polymer. The probe of this invention can rapidly and sensitively recognize adenosine triphosphate (ATP). It can also be used to construct fluorescent hydrogels by mixing this material with κ-carrageenan; the hydrogels exhibit a sensitive response to ATP and good self-healing ability.

This composition comprises: at least one buffer selected from the group consisting of 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid and 2-morpholinoethanesulfonic acid; and a capping reagent containing a capping compound capable of bonding to a terminal of a transcription reaction product, wherein the buffer concentration is 5-35 mM, and the pH is 5.0-7.5.

The invention belongs to the technical field of nerve cell culture, and particularly relates to application of a culture medium in co-culture of SVGP12 and HMEC-1. The culture medium is based on a mixture of DMEM and MCDB 131 according to a ratio of 1: 1, HEPES, Glutamax, N2, G-5, B-27, hEGF, penicillin-streptomycin and fetal calf serum are added, and the components are prepared according to a specific final concentration. When being applied to a Transwell co-culture model, the culture medium can meet the nutritional requirements of two cells at the same time, maintain the normal growth and form of the cells, and promote the transmission of functional signal molecules. Experiments show that the culture medium can significantly up-regulate expression of ABCG2, CYP2D6, CYP1B1 and CYP2E1 in HMEC-1 (P is less than 0.05), provides a reliable in-vitro model for researching an interaction mechanism of two cells, and assists drug screening and disease simulation.

The invention belongs to the technical field of nerve cell culture, and particularly relates to a co-culture method of SVGP12 and HMEC-1 cells. The method comprises the following steps: firstly, preparing a co-culture medium, on the basis of mixing DMEM and MCDB 131 according to a volume ratio, adding HEPES, Glutamax, N2, G-5, B-27, hEGF, penicillin-streptomycin and fetal calf serum, respectively adopting products with corresponding goods numbers for each component, and controlling specific final concentrations; and then respectively inoculating SVGP12 and HMEC-1 into culture flasks, culturing by using the co-culture medium, and carrying out passage. The method can realize indirect communication of two cells in a shared microenvironment, provides a reliable tool for researching a regulation mechanism of SVGP12 on HMEC-1, and also provides a reference for optimization design of a co-culture system.

The invention discloses a preparation method of a ferritin-based magnetic resonance nanoprobe targeting hepatic stellate cells, which comprises the following steps: S1, preparing an HEPES salt solution with the working concentration of 10 mmoL / L, and adjusting the pH value to 8.3 by using a NaOH solution; s2, potassium chloroplatinite powder is dissolved in the HEPES salt solution in the S1, a ferritin solution is added, then the mixture is placed on a magnetic stirrer to be stirred for 1 h at the room temperature, and standing is conducted for 5 h. According to the preparation method of the hepatostellate cell targeting ferritin-based magnetic resonance nanoprobe, the ferritin-based magnetic resonance nanoprobe is endowed with good MRI T1 imaging ability after loading of metal manganese ions and platinum, and non-specific recognition of a ferritin receptor in a liver is shielded by modifying excessive double-active ester polyethylene glycol; then, through mediation of the specific targeting molecule RGD peptide, the nanoprobe is efficiently delivered to the fibrosis liver, the interception effect of normal liver tissue is avoided, the imaging effect is greatly enhanced, and imaging diagnosis of mouse hepatic fibrosis can be achieved.

The application discloses a [2.2] cyclophane coumarin fluorescent probe, a preparation method and a method for detecting chiral molecules, and the fluorescent probe comprises a fluorescent probe structure, and the fluorescent probe structure is shown as formula (I). The [2.2] cyclophane skeleton is introduced into the coumarin molecule, and the fluorescent probe can be applied to fluorescence recognition of chiral molecules. The fluorescent probe can chemoselectively and enantioselectively recognize histidine in a mixed solution of tetrahydrofuran and HEPES aqueous solution, and can quantitatively detect the concentration of histidine and the ee value of histidine. The probe is an achiral fluorescent probe, can reduce the difficulty of separation of chiral compounds, greatly reduces the synthesis cost of the fluorescent probe, and opens up a new amino acid recognition probe.

This invention discloses a method for screening catalysts for the oxygen evolution reaction based on electrochemiluminescence quenching. The catalyst to be tested is loaded onto the working electrode, and then Ru(bpy)3 is added. 2+ In the electrolyte of / Hepes, applying an anodic potential to excite ECL accelerates the OER to generate O2, and O2 quenches the ECL signal. The signal intensity is negatively correlated with the catalytic activity. This method can be used for parallel detection on a single-electrode imaging array, achieving high throughput, visualization, and quantitative screening. It is applicable to various OER catalysts such as polyoxometalates and oxides, and has the advantages of being fast, low-cost, and easy to operate.

This invention discloses a peroxynitroso anion fluorescent probe with AIE effect, its synthesis method, and its applications. The near-infrared fluorescent probe TPS was designed and synthesized in this invention. It is nearly colorless under ultraviolet light irradiation but emits bright yellow fluorescence upon reaction with peroxynitroso anions. Detection showed that TPS reacts with ONOO in a HEPES:acetonitrile = 1:1 system. ‑ The response time was 100 min, after adding 120 uL of 0.5×10 ‑3 mol·L ‑1 ONOO ‑ The reaction is complete. TPS exhibits high specificity, reacting only with peroxynitrosoanion among various anions, demonstrating high sensitivity. TPS reacts with ONOO at pH 4-8. ‑ The ion response exhibits good stability, and TPS is effective against ONOO. ‑ The response exhibits good linearity, with a detection limit of 0.08232. µmol·L ‑1 Probe TPS and ONOO ‑ The reaction, after different concentrations of THF, exhibited a typical AIE effect.

The invention discloses a single-molecule fluorescence immunoassay kit for detecting dry plasma spot GFAP protein and a preparation method, and belongs to the technical field of immunodetection. The kit provided by the invention comprises an anti-GFAP mouse monoclonal antibody coated magnetic bead reagent, a biotinylated anti-GFAP mouse monoclonal antibody reagent, a streptavidin-beta-galactosidase reagent, a resorufin beta-D-galactoside reagent, a dry plasma spot eluent, a GFAP sample diluent, a GFAP calibration product, a GFAP quality control product and dry plasma spot filter paper. The dry plasma spot eluent contains PEG (Polyethylene Glycol) 6000 with the concentration of 15g / L to 25g / L, NaCl with the concentration of 15g / L to 20g / L, HEPES with the concentration of 11g / L to 12g / L, Triton X-100 with the concentration of 10g / L to 12g / L, EDTA (Ethylene Diamine Tetraacetic Acid) with the concentration of 4.5 mmol / L to 5.5 mmol / L, EGTA (Ethylene Glycol Terephthalate) with the concentration of 1.5 g / L to 2.0 g / L and BSA (Bovine Serum Albumin) with the concentration of 1.2 g / L to 1.8 g / L, and the pH value is adjusted to 7.3 to 7.5 by NaOH; aiming at the bottleneck problem of trace GFAP protein detection in a dried plasma spot sample, the detection sensitivity, accuracy and filter paper applicability are improved by optimizing an eluent formula, diluent components and a detection system.

A linearized mRNA preparation system, use thereof, and a preparation method for preparing mRNA by using same. A linearization buffer system comprises: 0-150 mM Tris-Ac or phosphate, 0.5-150 mM divalent metal ions or trivalent metal ions, 0.1-0.75 U / μL restriction endonuclease, and 0-7.5 mM spermidine. The present invention provides a transcription system, which has higher translation efficiency and lower dsRNA generation compared with a conventional HEPES / Tris system, and a phosphate system can be used simultaneously with the novel All In One transcription system.

A perfusion stock composition, for preserving a donor organ for transplantation, comprising: a source of 60 to 100 mM Na+; a source of 10 to 20 mM K+; a source of 5 to 10 mM Mg2+; a source of 0.25 to 0.75 mM Ca2+; 10 to 40 mM Tris(hydroxymethyl)aminomethane hydrochloride (Tris or THAM), 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), 3-(N-morpholino)propanesulfonic acid (MOPS), 2-(N-morpholino)ethanesulfonic acid (IviES), N,N-bis-(2-hydroxyethyl)-2-aminoethanesulfonic acid (BES), or N / -tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid (TES); a source of 10 to 30 mM HCO3; 1 to 30 mM glucose; 1 to 20 U / L insulin; 1 to 10 mM fructose diphosphate or a salt thereof, 1 to 40 mM aspartate or glutamate; 1 to 10 mM adenosine, cAMP or cGMP; 1 to 10 mM reduced glutathione; and 30 to 100 mM lactobionate or mannitol; and, optionally, a diluent. The present disclosure also provides a perfusion composition, a kit, a method, and a perfusion apparatus, each related to the perfusion stock composition.

The application provides a method for degrading chlorohydrocarbon in water by using illite reinforced ball-milling sulfidation micro zero-valent iron, which comprises the following steps: first, a buffer solution with pH=7 is prepared by using 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, and an oxygen-free aeration treatment is performed; then, illite and S-mZVI bm are mixed to prepare a particle mixture, and the particle mixture is sealed and stored in the buffer solution with pH=7; finally, chlorohydrocarbon pollutants are added into the particle mixture of illite and S-mZVI bm , and a sealed oscillation reaction is performed, so that the concentration of the chlorohydrocarbon in the reaction system is determined. The application directly reinforces the activity of S-mZVI bm material by using illite, which is a clay mineral widely distributed in nature, so that the defects of S-mZVI bm , such as large particle size, slow degradation rate and decreased activity in the later reaction stage, are effectively solved, and the long-term reaction activity of the particles is ensured. The raw materials are green and environmentally friendly, the process is simple, and the materials are easy to obtain.

The invention belongs to the technical field of cell culture, and particularly relates to a culture method and application of human astrocytes. According to the culture solution provided by the invention, DMEM (Dulbecco Modified Eagle Medium) and MCDB 131 are mixed according to a specific ratio to form a basic culture medium, and functional additives of HEPES, Glutamax, N2, G-5, B-27, hEGF (Human Epidermal Growth Factor) and the like with accurate concentration and fetal calf serum with proper concentration are matched. The culture solution can effectively improve the vitality and proliferation efficiency of SVGP12 cells, guarantees the growth stability and morphological consistency of the cells, can provide a high-quality and reliable cell source for the application of human astrocytes in the fields of neurobiology research, neurological disease mechanism exploration, drug screening and the like, and has important practical value.

This invention provides a culture medium for closed-system culture of mesenchymal stem cells, comprising the following components: 1% (v / v) human platelet lysate, 10-15 g / L basal culture medium powder, 15-20 mM HEPES, 1.0-2.0 g / L sodium bicarbonate, and 0.1-1.0 g / L sodium chloride; wherein the basal culture medium powder includes non-essential amino acids, D-glucose, L-glutamine, specific growth factors, inorganic salts, and vitamins. Using the culture medium provided by this invention for mesenchymal stem cell culture eliminates the need for exogenous CO2, reducing equipment costs and process complexity; furthermore, it results in cell viability ≥97%, more concentrated particle size distribution, and more stable expression of surface markers, which is beneficial for large-scale production.

A perfusion stock composition, for preserving a donor organ for transplantation, comprising: a source of 60 to 100 mM Na+; a source of 10 to 20 mM K+; a source of 5 to 10 mM Mg2+; a source of 0.25 to 0.75 mM Ca2+; 10 to 40 mM Tris(hydroxymethyl)aminomethane hydrochloride (Tris or THAM), 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), 3-(N-morpholino)propanesulfonic acid (MOPS), 2-(N-morpholino)ethanesulfonic acid (IviES), N,N-bis-(2-hydroxyethyl)-2-aminoethanesulfonic acid (BES), or N / -tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid (TES); a source of 10 to 30 mM HCO3; 1 to 30 mM glucose; 1 to 20 U / L insulin; 1 to 10 mM fructose diphosphate or a salt thereof; 1 to 40 mM aspartate or glutamate; 1 to 10 mM adenosine, cAMP or cGMP; 1 to 10 mM reduced glutathione; and 30 to 100 mM lactobionate or mannitol; and, optionally, a diluent. The present disclosure also provides a perfusion composition, a kit, a method, and a perfusion apparatus, each related to the perfusion stock composition.

The invention relates to the technical field of bioengineering, in particular to a culture medium and a method for constructing auditory neuroma organs. According to the culture medium, Neurobase Medium and DMEM (Dulbecco Modified Eagle Medium) / F12 are used as a basic culture medium; the feed additive comprises a N2 additive, a B27 additive, L-alanyl-L-glutamine, an antibiotic, HEPES, glucose, beta-mercaptoethanol, a cell growth factor, MEM NEAA and a TGF-beta inhibitor. The cell growth factor is EGF (Epidermal Growth Factor) or FGF-2. The culture medium provided by the invention can effectively improve the construction efficiency of the auditory neuroma organoid, and the constructed auditory neuroma organoid can be used as a reliable in-vitro substitution model, is used for marker detection, drug screening and individualized medical exploration in basic research and clinical transformation research, and has important application value.

The invention provides an Escherichia coli culture medium capable of efficiently promoting growth and a preparation method thereof, and belongs to the technical field of microbial culture mediums. The Escherichia coli culture medium per liter comprises the following components: 10 to 15 g of tryptone, 5 to 10 g of yeast extract, 2.5 to 3 g of glucose, 3 to 5 mL of glycerin, 4 to 6 g of sodium chloride, 5 to 10 g of dipotassium phosphate trihydrate, 1 to 5 g of potassium dihydrogen phosphate, 0.1 to 1 g of magnesium sulfate heptahydrate, 0.01 to 0.1 g of calcium chloride, 0.1 to 1 g of L-glutamine, 0.01 to 0.02 g of vitamin B group mixture, 0.5 to 2 g of betaine and an HEPES buffer solution, and the final concentration of HEPES is 10 mM. Compared with an LB culture medium, the escherichia coli culture medium has the advantages that the growth rate is increased by 55.6%, the recombinant protein expression quantity is increased by 59.1%, and the plasmid extraction yield is increased by 69.4%.

The invention relates to a method for synthesizing spherical nucleic acid by reducing gold trichloride through alkynyl-modified DNA. The method comprises the following steps: preparing a gold trichloride aqueous solution, an alkynyl-modified DNA solution, a poloxamer 188 aqueous solution and an HEPES solution; adding a gold trichloride aqueous solution, an alkynyl modified DNA solution and a poloxamer 188 aqueous solution into an HEPES solution, and reacting to obtain the spherical nucleic acid of the DNA modified gold nanoparticles. According to the method, the alkynyl-modified DNA is used for reducing gold trichloride to synthesize the spherical nucleic acid in one step for the first time, the use of the alkynyl-modified DNA realizes rapid one-step synthesis of the DNA-modified gold nanoparticles, and the synthesis and modification processes of the spherical nucleic acid are greatly simplified. The preparation method is simplified, and the reaction time is shortened.

The application discloses an exosome protection solution and a preparation method thereof, and relates to the technical field of biology. The exosome protection solution comprises the following components in terms of concentration: 0.01-0.1 g / mL of HEPEs, 0.01-0.1 g / mL of trehalose, 0.01-0.1 g / mL of mannitol, 8-10 g / L of recombinant human blood albumin, 0.01-0.1 g / mL of glycine and 0.01-0.1 g / mL of poloxamer 188. The preparation method of the exosome protection solution comprises the following steps: S1, dissolving HEPEs in pure water, and stirring to prepare an HEPEs buffer solution; S2, adding trehalose, mannitol and glycine into the HEPEs buffer solution, and forming a mixed solution after complete dissolution; S3, adding recombinant human blood albumin and poloxamer 188 into the mixed solution and stirring uniformly; and S4, mixing the protection solution with exosomes at a ratio of 1:1. The exosome protection solution provided by the application can store exosomes in a temperature range of-80 DEG C to 4 DEG C, and meets the requirement of long-term storage of exosomes under different temperature environments. The protection solution can effectively improve the stability and integrity of exosomes in the long-term storage process.

The invention belongs to the technical field of biological detection, and particularly relates to a sample treating fluid for detecting bordetella pertussis, a detection method, a product and application. The sample treatment liquid comprises a lysis working solution (WLB) and a post-treatment diluent (PD), the WLB is composed of choline tartrate, betaine, glycerin, TCEP, IGEPAL CA-630, HEPES and non-enzyme deionized water, and the PD is composed of bovine serum albumin and non-enzyme deionized water. The kit is suitable for common clinical samples such as sputum and nasopharynx swab eluent, bordetella pertussis can be efficiently split, target nucleic acid is fully released, and meanwhile matrix inhibitors such as mucoprotein, heme and protease in the samples are effectively removed. The kit is simple and convenient to operate, free of complex purification steps, high in detection sensitivity, good in reproducibility, high in specificity and free of cross reaction, and provides reliable technical support for early precise diagnosis and epidemic prevention and control of pertussis.

The invention belongs to the technical field of cell culture, and particularly relates to a culture method and application of human perinervous cells. The culture method comprises the following steps: inoculating HBVP into a compound basic culture medium which is prepared from DMEM and MCDB 131 mixed in a ratio of 1: 1, accurately adding HEPES with the final concentration of 10 mM, 1% (v / v) Glutamax, 1% (v / v) N2, 1% (v / v) G-5, 1% (v / v) B-27, 10 ng / mL hEGF, 5% (v / v) fetal calf serum and 1% (v / v) penicillin-streptomycin into the compound basic culture medium to form a special HBVP culture solution, and the culture solution can provide an optimal growth microenvironment for HBVP. The cell culture medium can effectively promote continuous and stable proliferation of cells and maintain high activity, can be used as a high-quality cell source for HBVP related basic research, preclinical research and potential application, and has good practicability and popularization value.

Anti-SIRPa antibody formulation, suitable for parenteral administration, comprising: an anti-SIRPα antibody that inhibits signaling via the SIRPα-CD47 axis at a concentration between approximately 20 mg / ml and approximately 100 mg / ml; a buffer component selected from the group consisting of approximately 20 mM L-histidine and 20 mM sodium phosphate; a disaccharide comprising an α-glycosidic bond and present at a concentration of approximately 8 wt. / vol.%; approximately 0.01 wt% polysorbate 20; and a pH value of approximately 5.7 to approximately 6.3; which is free of glycine, arginine, carbonate, HEPES, citrate and acetate.

The invention relates to the technical field of cell cryopreservation, and discloses a blastocyst vitrification refrigerating fluid set and application, the refrigerating fluid set comprises a basic buffer solution, a composite permeable protective agent, a non-permeable protective agent, a thermosensitive macromolecule regulating agent, a molecular state locking agent, a zwitterionic osmotic pressure buffer agent, a cell apoptosis inhibitor and an efficient antioxidant, the invention further provides a preparation method of the blastocyst vitrification refrigerating fluid set. The preparation method comprises the following steps: weighing HEPES in a clean container; gradually adding ethylene glycol and dimethyl sulfoxide into the basic buffer solution according to a ratio of 1: 1; slowly adding trehalose into the mixed liquid; slowly adding poloxamer into the transparent solution; then adding chondroitin sulfate; proline is gradually added; the preparation method comprises the following steps: adding a generic caspase inhibitor Z-VAD-FMK; and then adding the L-glutathione. According to the method disclosed by the invention, the blastocyst is subjected to cryoprotection by adopting the impermeable protective agent trehalose, so that the cell survival rate is remarkably improved.

The invention provides a preparation method and application of PD-L1 targeted STING agonist loaded lipid nanoparticles, and belongs to the technical field of biological medicines. The preparation method comprises the following steps: dissolving cholesterol, HSPC and DSPE-PEG2000 in preheated absolute ethyl alcohol, uniformly mixing, then rapidly injecting into a vortex preheated PBS solution, heating vortex in a water bath, dialyzing and extruding to obtain blank nano-liposome; the preparation method comprises the following steps: dissolving an STING agonist in an HEPES / NaCl solution, adding a PEI solution, carrying out vortex oscillation by using a vortex oscillator, standing at room temperature to obtain an STING agonist-PEI complex, mixing the STING agonist-PEI complex with a blank nano-liposome, carrying out vortex oscillation, standing at room temperature, and dialyzing to remove the free STING agonist to obtain the STING agonist-coated lipid nanoparticles; dSPE-PEG-NHS is dissolved in ultrapure water, cooling is carried out to room temperature, then a PD-L1 antibody is added, incubation and dialysis are carried out, and immune lipid microspheres coupled with the PD-L1 antibody are obtained; and mixing the lipid nanoparticles wrapped with the STING agonist with the immune lipid microspheres coupled with the PD-L1 antibody, carrying out vortex oscillation, standing at room temperature, and dialyzing to obtain the lipid nanoparticles of PD-L1 targeted loading STING agonist.