The invention provides a method and a device for acquiring node transmission information on a transmission path. The method comprises: network time of a source node and a target node are synchronized, when the source node transmits a Ping message to the target node, the transmission time of the message is recorded in the IP header option field of the Ping message; after the Ping message reaches the target node, at least one of the following information is recorded: the IP header option field which copies the transmission time to the Ping reply message, the time when the target node receives the Ping message, the time when the Ping reply message is generated, the time when the Ping reply message is transmitted, the CPU occupancy rate the memory occupancy rate, the interface type and MTU when the Ping reply is generated. At least one of the above information is transmitted to the source node; and according to the transmission time of the source node, the receiving time of the target node, the generation time and the transmission time, the delay information is calculated and CPU utilization rate, the memory occupancy rate, the interface type and the MTU are extracted. The invention can easily and effectively acquire relevant delay information system information and interface information of each node in the transmission path,.