919 results about "Hybrid" patented technology

The invention relates to a composition for identifying epinephelus fuscoguttatus, epinephelus coioides and hybrid offspring and application, and belongs to the field of molecular biology, the composition comprises a pair of primers and a group of probes, and the sequences are shown as SEQ ID NO.1-4. The invention further provides a kit for identifying the epinephelus fuscoguttatus, the epinephelus coioides and the hybrid offspring thereof, and the kit is used for identifying the epinephelus fuscoguttatus and the hybrid offspring of the epinephelus coioides and the hybrid offspring of the epinephelus coioides and the hybrid offspring of the epinephelus coioides. The kit comprises the composition disclosed by the invention. The composition can be used for rapidly identifying epinephelus fuscoguttatus, epinephelus coioides and hybrid filial generation gold and tiger hybrid spots of the epinephelus coioides, the epinephelus coioides and the hybrid filial generation gold and tiger hybrid spots of the epinephelus coioides and the epinephelus coioides and the hybrid filial generation gold and tiger hybrid spots of the epinephelus

The invention provides an Indel molecular marker primer pair, a kit and a method for identifying Yichang orange, and belongs to the technical field of biochemistry, the Indel molecular marker primer pair comprises at least one of an InDel-2 primer pair and an InDel-3 primer pair; the sequences of the InDel-2 primer pair are as shown in SEQ ID No. 1 and SEQ ID No. 2, and the sequences of the InDel-3 primer pair are as shown in SEQ ID No. 3 and SEQ ID No. 4. The Indel molecular marker primer pair or the kit disclosed by the invention can specifically recognize the Yichang oranges, realizes efficient and accurate identification of the Yichang oranges and filial generations thereof, and has wide application prospects in the aspects of citrus germplasm resource protection, variety right and interest maintenance, breeding selection, market supervision and the like. The method for identifying the Yichang oranges can accurately identify the Yichang oranges and the filial generations of the Yichang oranges, has high specificity, economical efficiency and high efficiency, is an accurate, economical and efficient hybrid identification means, can effectively identify the variety of the Yichang oranges and the hybrids of the Yichang oranges, and provides powerful technical support for scientific research and breeding work.

The invention belongs to the technical field of crop genetic breeding, and particularly relates to a rice hybrid male sterility site HMS10 co-segregation molecular marker and application thereof. The method comprises the following steps: hybridizing and backcrossing an anti-thermo-sensitive type genic male sterile line Yannong S serving as a female parent with a normal fertile material Yannong HB (serving as a recurrent male parent), finely positioning to a site HMS10 for controlling hybrid male sterility of rice in a physical interval of-60kb of a chromosome 10 of the rice by utilizing a high-generation backcross large population, and developing a co-separation molecular marker IDHMS10. The molecular marker IDHMS10 is utilized, through a molecular breeding strategy, a positive temperature-sensitive male genic male sterile line Quan 211S with excellent comprehensive agronomic traits is transformed into a practical rice hybrid male sterile line Quan 211HS with fertility not affected by light and temperature, and the IDHMS10 is used for purity identification of the hybrid male sterile line, so that the molecular marker IDHMS10 has great significance in solving the seed production risk of two-line hybrid rice and improving the yield of the hybrid male sterile line. And the method has important significance in enriching practical sterile resource types and the like.

A novel canola variety designated 4PRSD48R and seed, plants and plant parts thereof. Methods for producing a canola plant that comprise crossing canola variety 4PRSD48R with another canola plant. Methods for producing a canola plant containing in its genetic material one or more traits introgressed into 4PRSD48R through backcross conversion and / or transformation, and to the canola seed, plant and plant part produced thereby. Hybrid canola seed, plant or plant part produced by crossing the canola variety 4PRSD48R or a locus conversion of 4PRSD48R with another canola variety.

A novel canola variety designated 4PYYM82A and seed, plants and plant parts thereof. Methods for producing a canola plant that comprise crossing canola variety 4PYYM82A with another canola plant. Methods for producing a canola plant containing in its genetic material one or more traits introgressed into 4PYYM82A through backcross conversion and / or transformation, and to the canola seed, plant and plant part produced thereby. Hybrid canola seed, plant or plant part produced by crossing the canola variety 4PYYM82A or a locus conversion of 4PYYM82A with another canola variety.

The invention discloses an artificial breeding method of epinephelus fuscoguttatus and pink pterus coerulescens hybrid species, which comprises the following steps: screening healthy epinephelus fuscoguttatus as a female parent, screening healthy pink pterus coerulescens as a male parent, and carrying out enhanced breeding on the male parent and the female parent, the gonad maturation of the parent is promoted; the method comprises the following steps: collecting egg liquid of a female parent and semen of a male parent, inseminating to obtain a fertilized egg, incubating the fertilized egg to obtain a hybrid spot, performing authenticity identification on the hybrid spot by using one or more of sequences SEQ ID NO.1-SEQ ID NO.8, and determining that the hybrid spot inherits genetic information of the female parent and the male parent. The technical blank of crossbreeding of the pink pink bass as the parent grouper is filled, so that the hybrid obtained by hybridizing the epinephelus fuscoguttatus and the pink pink bass is reliable in authenticity, and inherits genetic information of male and female parents on the molecular level.

A novel maize variety designated X18V354 and seed, plants and plant parts thereof are produced by crossing inbred maize varieties. Methods for producing a maize plant by crossing hybrid maize variety X18V354 with another maize plant are disclosed. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into X18V354 through backcrossing or genetic transformation, and to the maize seed, plant and plant part produced thereby are described. Maize variety X18V354, the seed, the plant produced from the seed, and variants, mutants, and minor modifications of maize variety X18V354 are provided. Methods for producing maize varieties derived from maize variety X18V354 and methods of using maize variety X18V354 are disclosed.

The invention discloses a corn genetic performance prediction method fusing multiple environmental factors. The corn genetic performance prediction method comprises the following steps: acquiring a parent genotype data matrix, a hybrid phenotype data vector and a multi-environmental time sequence climate data tensor; a climate hysteresis effect matrix is constructed, and hysteresis influence degrees of different climate factors on corn growth and development are quantified; constructing an environment time-space relation matrix and an enhanced genome relation matrix considering non-equal contributions of sections; constructing a linear hybrid prediction model fusing the space-time environment information; estimating a variance component through a constraint maximum likelihood method, and solving model parameters based on a Henderson hybrid model equation; and predicting the phenotypic character of the to-be-predicted corn hybrid in the target environment by using the solving parameters. According to the method, the genotype data and the multi-environment time sequence climate data are fully utilized, the genotype effect, the environment space-time effect and the interaction effect of the genotype effect and the environment space-time effect are effectively integrated, and the accuracy of corn hybrid phenotype prediction is remarkably improved.

The invention discloses a primer for identifying authenticity of distant hybridization offspring of clausena lansium and citrus, the primer can amplify target bands of 270bp, 296bp and 235bp in the distant hybridization offspring of clausena lansium and citrus, and the hybridization offspring with the target bands of 270bp, 296bp and 235bp is the real hybridization offspring. The method provided by the invention can quickly and accurately identify the authenticity of the distant hybridization offspring of the clausena lansium and the citrus, has the characteristics of short identification time, high accuracy, strong specificity and the like, provides an effective basis for identification and research of the distant hybridization offspring of the clausena lansium and the citrus, and is beneficial to genetic improvement of citrus varieties.

The invention provides a method for cultivating hybrid fries of Jinhu and tiger. The method comprises the following steps: selecting a breeding environment; breeding and ripening female parents; management and control before oviposition; insemination; hatching fertilized eggs; fry rearing. According to the method, in the female parent breeding and ripening stage, specific biological bait is controlled to be fed in stages, feeding of the parent fish can be promoted, growth can be accelerated, in cooperation with specific compound feed in the management and control stage before oviposition, favorable conditions are created for subsequent oviposition, and the quality and number of ova can be improved. Low-frequency ultrasonic assisted fertilization is introduced in the artificial insemination stage, so that the activity of ovum sperms can be obviously improved, and the fertilization rate is increased; in the fertilized egg hatching stage, intermittent ultrasound is assisted, the hatching rate can be increased, and the seedling yield of the golden-tiger hybrid spots can be increased; after hatching, transferring into a specific culture pond, slowly releasing beneficial bacteria, improving the adaptability of the fry, adsorbing harmful substances, reducing pathogenic bacteria, stabilizing water quality, promoting the growth of the fry, improving the survival rate and reducing the malformation rate.

A novel maize variety designated X08V033 and seed, plants and plant parts thereof are produced by crossing inbred maize varieties. Methods for producing a maize plant by crossing hybrid maize variety X08V033 with another maize plant are disclosed. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into X08V033 through backcrossing or genetic transformation, and to the maize seed, plant and plant part produced thereby are described. Maize variety X08V033, the seed, the plant produced from the seed, and variants, mutants, and minor modifications of maize variety X08V033 are provided. Methods for producing maize varieties derived from maize variety X08V033 and methods of using maize variety X08V033 are disclosed.

A novel canola variety designated 4PWGT51R and seed, plants and plant parts thereof. Methods for producing a canola plant that comprise crossing canola variety 4PWGT51R with another canola plant. Methods for producing a canola plant containing in its genetic material one or more traits introgressed into 4PWGT51R through backcross conversion and / or transformation, and to the canola seed, plant and plant part produced thereby. Hybrid canola seed, plant or plant part produced by crossing the canola variety 4PWGT51R or a locus conversion of 4PWGT51R with another canola variety.

A novel canola variety designated 4PWSP65R and seed, plants and plant parts thereof. Methods for producing a canola plant that comprise crossing canola variety 4PWSP65R with another canola plant. Methods for producing a canola plant containing in its genetic material one or more traits introgressed into 4PWSP65R through backcross conversion and / or transformation, and to the canola seed, plant and plant part produced thereby. Hybrid canola seed, plant or plant part produced by crossing the canola variety 4PWSP65R or a locus conversion of 4PWSP65R with another canola variety.

Hybrid tomato varieties designated ‘E15A43014’, ‘E15B42750’ and ‘E15M43066’ are disclosed. The disclosure relates to, e.g., seeds or plants thereof, as well as to methods for producing other tomato lines, cultivars, or hybrids derived from the hybrid tomato varieties selected from ‘E15A43014’, ‘E15B42750’, and ‘E15M43066’.

The present disclosure provides for a hybrid salogel, methods of making hybrid salogels, and systems using hybrid salogels.

Provided is a canola variety designated 20GG1406N and seed, plants and plant parts thereof produced from a cross of inbred varieties. Methods for producing a canola variety comprise crossing canola variety 20GG1406N with another canola plant. Methods for producing a canola plant containing in its genetic material one or more traits introgressed into 20GG1406N through backcross conversion and / or transformation, and to the canola seed, plant and plant part produced thereby are described. Canola variety 20GG1406N, the seed, the plant produced from the seed, plant parts and variants, mutants, and minor modifications of canola variety 20GG1406N are disclosed.

A novel maize variety designated X95V292 and seed, plants and plant parts thereof are produced by crossing inbred maize varieties. Methods for producing a maize plant by crossing hybrid maize variety X95V292 with another maize plant are disclosed. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into X95V292 through backcrossing or genetic transformation, and to the maize seed, plant and plant part produced thereby are described. Maize variety X95V292, the seed, the plant produced from the seed, and variants, mutants, and minor modifications of maize variety X95V292 are provided. Methods for producing maize varieties derived from maize variety X95V292 and methods of using maize variety X95V292 are disclosed.

The invention discloses an artificial cultivation method of a hybrid of epinephelus trifoliatus and epinephelus lanceolatus, which comprises the following steps: taking healthy epinephelus trifoliatus as female parent fish, and carrying out cultivation strengthening and artificial ripening to obtain strengthened female parent fish; healthy epinephelus lanceolatus is used as male parent fish, and sexual mature male parent fish is obtained through natural sexual maturity; the ovulation time and the spermiation time are consistent; collecting egg liquid and seminal fluid, inseminating to obtain fertilized eggs, and performing embryo incubation and illumination regulation and control cultivation after opening to obtain hybrid spots; carrying out authenticity identification on the hybrid spots by utilizing a primer pair, and determining that the hybrid spots inherit genetic information of the female parent fish and the male parent fish; the primer pair is a sequence pair composed of SEQ ID NO. 1 and SEQ ID NO. 2, and a sequence pair composed of SEQ ID NO. 3 and SEQ ID NO. 4. The technical blank of crossbreeding by taking the epinephelus trifoliatus as the parent epinephelus is filled, and the authenticity of the hybridized spots is reliable.

The invention provides an artificial seedling method of a tiger-dragon hybrid spot, and relates to the field of aquaculture. An artificial seedling method of a tiger-dragon hybrid spot comprises the following steps: (1) selecting an outdoor high-level pond, selecting tiger-dragon hybrid spot fry over 7 days old, and putting the tiger-dragon hybrid spot fry into the high-level pond; (2) feeding rotifers from 7 days to 14 days old, and inoculating protein nucleus Chlorella liquid and halogenated Dunaliella liquid in the pond at the same time; feeding Artemia and Mongolian monilopsis from 15 days to 30 days old; feeding Mongolian monilopsis and yellow mealworm from 31 days to 50 days old; (3) when encountering a rainstorm, feeding a nutrient within 24 hours after the rain. The invention has a high survival rate of seedlings, a large size of the obtained fry, a good seedling effect, and a low mutation rate, and better guarantees the seedling quality of the tiger-dragon hybrid spot. The obtained tiger-dragon hybrid spot fry has stronger resistance, and the subsequent breeding can better cope with the adverse weather effects, which can effectively reduce the breeding risk of the tiger-dragon hybrid spot and significantly improve the breeding efficiency of the tiger-dragon hybrid spot.

The invention discloses enterobacter mori capable of resisting fish aeromonas and application of the enterobacter mori, the bacterial strain is classified and named as enterobacter mori SCH0241, the enterobacter mori SCH0241 is preserved in the China Center for Type Culture Collection on October 17, 2024, and the preservation number is CCTCC NO: M 20242236. The enterobacter mori SCH0241 disclosed by the invention is derived from the intestinal tract of high-stress-resistance healthy hybrid crucian carassius auratus No. 2, has good contact dependence antibacterial activity on aeromonas veronii, and can improve the capability of resisting aeromonas veronii infection of zebra fish. Through construction of an insertion mutation library and phenotype screening, it is found that a gene cluster of SCH0241, which exerts contact inhibition toxicity, is T6SS-2. The enterobacter mori SCH0241 disclosed by the invention provides a new powerful tool for biological prevention and treatment of fish diseases caused by aeromonas veronii.

The invention belongs to the technical field of plant biology, and particularly relates to a gene for regulating and controlling powdery mildew resistance of cucumbers and application of the gene, two cswrky31 mutants are obtained based on a cucumber Tnt1 reverse transcription transposon mutant library, and the two cswrky31 mutants both show high susceptibility to powdery mildew bacteria. A target gene Csa5G551250 which is directly regulated and controlled by CsWRKY31 is screened by virtue of a high-throughput transcriptome sequencing technology and a DNA affinity purification sequencing technology in combination with a yeast one-hybrid experiment. A dual luciferase report experiment shows that the CsWRKY31 positively regulates and controls the expression of the Csa5G551250, and the overexpression of the Csa5G551250 gene promotes the resistance of the cucumber to powdery mildew bacteria. A new reference gene resource is provided for cultivation of cucumber disease-resistant varieties, and a new thought is provided for molecular mechanism research of transcription factor mediated plant immunity.

A novel maize variety designated X95V285 and seed, plants and plant parts thereof are produced by crossing inbred maize varieties. Methods for producing a maize plant by crossing hybrid maize variety X95V285 with another maize plant are disclosed. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into X95V285 through backcrossing or genetic transformation, and to the maize seed, plant and plant part produced thereby are described. Maize variety X95V285, the seed, the plant produced from the seed, and variants, mutants, and minor modifications of maize variety X95V285 are provided. Methods for producing maize varieties derived from maize variety X95V285 and methods of using maize variety X95V285 are disclosed.

A novel maize variety designated X95V287 and seed, plants and plant parts thereof are produced by crossing inbred maize varieties. Methods for producing a maize plant by crossing hybrid maize variety X95V287 with another maize plant are disclosed. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into X95V287 through backcrossing or genetic transformation, and to the maize seed, plant and plant part produced thereby are described. Maize variety X95V287, the seed, the plant produced from the seed, and variants, mutants, and minor modifications of maize variety X95V287 are provided. Methods for producing maize varieties derived from maize variety X95V287 and methods of using maize variety X95V287 are disclosed.

The invention relates to the field of distant hybridization breeding, and discloses an artificial breeding method for hybrid species of humpback perches and epinephelus fuscoguttatus, which comprises the following steps: selecting healthy humpback perches as female parent fishes, and screening healthy epinephelus fuscoguttatus as male parent fishes; carrying out intensive cultivation on the parents by means of nutrient enrichment and temperature regulation and control, and promoting synchronous development and maturation of gonads of the parents; after the parents reach the breeding state, eggs of the female parent fishes and seminal fluid of the male parent fishes are collected respectively, fertilized eggs are obtained through artificial insemination, incubation management such as temperature control and continuous oxygenation is conducted on the fertilized eggs, and finally hybrid grouper fries are obtained. The crossbreeding application of the humpback perch as the female parent and the epinephelus fuscoguttatus is realized, and the problems that in the traditional hybridization process, parent gonad development is asynchronous, and filial generations cannot develop are effectively solved; the bred hybrid provides technical guarantee for standardization and precision promotion of grouper crossbreeding, and creates favorable conditions for breeding new hybrid lines with excellent characters of parents.

The invention provides a breeding method of hypoxia-resistant golden tiger hybrid spots, which comprises the following steps: carrying out artificial insemination on a female parent of epinephelus coioides and a male parent of epinephelus coioides to obtain fertilized eggs, carrying out 2.8-3.2 mg / L hypoxia screening on the female parent for more than three generations, and ensuring that the suffocation point is less than or equal to 0.9 mg / L. Performing staged low-oxygen incubation on the fertilized eggs, gradually reducing dissolved oxygen in an embryonic period, a protointestinal period and a membrane emergence period, performing specific sound wave stimulation in an opening period of larva fish, performing low-oxygen-resistant phenotype screening in a larva period, performing intermittent low-oxygen intensive training in a juvenile fish period, applying water flow stimulation before training, and performing low-oxygen-resistant phenotype screening in a membrane emergence period; during the period, a fortified feed containing gamma-aminobutyric acid, heme iron and other components is fed. According to the method, through multi-link collaborative innovation, the hypoxia resistance of the Jinhu hybrid spots is effectively improved, the survival rate of a target group under 2.5 mg / L dissolved oxygen reaches 93%, the suffocation point is reduced to 0.75 mg / L, meanwhile, gill filament development and growth are promoted, and an efficient technical scheme is provided for aquatic product breeding.

A novel maize variety designated X13R107 and seed, plants and plant parts thereof are produced by crossing inbred maize varieties. Methods for producing a maize plant by crossing hybrid maize variety X13R107 with another maize plant are disclosed. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into X13R107 through backcrossing or genetic transformation, and to the maize seed, plant and plant part produced thereby are described. Maize variety X13R107, the seed, the plant produced from the seed, and variants, mutants, and minor modifications of maize variety X13R107 are provided. Methods for producing maize varieties derived from maize variety X13R107 and methods of using maize variety X13R107 are disclosed.

The present invention relates to methods of breeding with spotted watermelon, Citrullus lanatus, lines and varieties. More specifically, methods of making homozygous-spotted diploid watermelon lines (HSDL) for use in breeding and methods of making homozygous-spotted tetraploid watermelon lines (HSDL) for use in breeding are described. Further, methods of producing 33%, 66%, or 100% spotted triploid hybrid watermelon plants, as well as methods of producing 50% or 100% spotted diploid hybrid watermelon plants, are described. This invention further relates to markers able to determine the percentage of spotted allele present in spotted watermelon lines and varieties. In addition, this invention relates to new and distinctive lines and varieties of watermelon with a spotted phenotype.

A novel maize variety designated X13V198 and seed, plants and plant parts thereof are produced by crossing inbred maize varieties. Methods for producing a maize plant by crossing hybrid maize variety X13V198 with another maize plant are disclosed. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into X13V198 through backcrossing or genetic transformation, and to the maize seed, plant and plant part produced thereby are described. Maize variety X13V198, the seed, the plant produced from the seed, and variants, mutants, and minor modifications of maize variety X13V198 are provided. Methods for producing maize varieties derived from maize variety X13V198 and methods of using maize variety X13V198 are disclosed.

A novel canola variety designated 4PVZT05R and seed, plants and plant parts thereof. Methods for producing a canola plant that comprise crossing canola variety 4PVZT05R with another canola plant. Methods for producing a canola plant containing in its genetic material one or more traits introgressed into 4PVZT05R through backcross conversion and / or transformation, and to the canola seed, plant and plant part produced thereby. Hybrid canola seed, plant or plant part produced by crossing the canola variety 4PVZT05R or a locus conversion of 4PVZT05R with another canola variety.

A novel maize variety designated X00V048 and seed, plants and plant parts thereof are produced by crossing inbred maize varieties. Methods for producing a maize plant by crossing hybrid maize variety X00V048 with another maize plant are disclosed. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into X00V048 through backcrossing or genetic transformation, and to the maize seed, plant and plant part produced thereby are described. Maize variety X00V048, the seed, the plant produced from the seed, and variants, mutants, and minor modifications of maize variety X00V048 are provided. Methods for producing maize varieties derived from maize variety X00V048 and methods of using maize variety X00V048 are disclosed.