557 results about "Hybrid" patented technology

The invention relates to a composition for identifying epinephelus fuscoguttatus, epinephelus coioides and hybrid offspring and application, and belongs to the field of molecular biology, the composition comprises a pair of primers and a group of probes, and the sequences are shown as SEQ ID NO.1-4. The invention further provides a kit for identifying the epinephelus fuscoguttatus, the epinephelus coioides and the hybrid offspring thereof, and the kit is used for identifying the epinephelus fuscoguttatus and the hybrid offspring of the epinephelus coioides and the hybrid offspring of the epinephelus coioides and the hybrid offspring of the epinephelus coioides. The kit comprises the composition disclosed by the invention. The composition can be used for rapidly identifying epinephelus fuscoguttatus, epinephelus coioides and hybrid filial generation gold and tiger hybrid spots of the epinephelus coioides, the epinephelus coioides and the hybrid filial generation gold and tiger hybrid spots of the epinephelus coioides and the epinephelus coioides and the hybrid filial generation gold and tiger hybrid spots of the epinephelus

The invention discloses an artificial breeding method of epinephelus fuscoguttatus and pink pterus coerulescens hybrid species, which comprises the following steps: screening healthy epinephelus fuscoguttatus as a female parent, screening healthy pink pterus coerulescens as a male parent, and carrying out enhanced breeding on the male parent and the female parent, the gonad maturation of the parent is promoted; the method comprises the following steps: collecting egg liquid of a female parent and semen of a male parent, inseminating to obtain a fertilized egg, incubating the fertilized egg to obtain a hybrid spot, performing authenticity identification on the hybrid spot by using one or more of sequences SEQ ID NO.1-SEQ ID NO.8, and determining that the hybrid spot inherits genetic information of the female parent and the male parent. The technical blank of crossbreeding of the pink pink bass as the parent grouper is filled, so that the hybrid obtained by hybridizing the epinephelus fuscoguttatus and the pink pink bass is reliable in authenticity, and inherits genetic information of male and female parents on the molecular level.

The invention discloses a corn genetic performance prediction method fusing multiple environmental factors. The corn genetic performance prediction method comprises the following steps: acquiring a parent genotype data matrix, a hybrid phenotype data vector and a multi-environmental time sequence climate data tensor; a climate hysteresis effect matrix is constructed, and hysteresis influence degrees of different climate factors on corn growth and development are quantified; constructing an environment time-space relation matrix and an enhanced genome relation matrix considering non-equal contributions of sections; constructing a linear hybrid prediction model fusing the space-time environment information; estimating a variance component through a constraint maximum likelihood method, and solving model parameters based on a Henderson hybrid model equation; and predicting the phenotypic character of the to-be-predicted corn hybrid in the target environment by using the solving parameters. According to the method, the genotype data and the multi-environment time sequence climate data are fully utilized, the genotype effect, the environment space-time effect and the interaction effect of the genotype effect and the environment space-time effect are effectively integrated, and the accuracy of corn hybrid phenotype prediction is remarkably improved.

The invention discloses an artificial cultivation method of a hybrid of epinephelus trifoliatus and epinephelus lanceolatus, which comprises the following steps: taking healthy epinephelus trifoliatus as female parent fish, and carrying out cultivation strengthening and artificial ripening to obtain strengthened female parent fish; healthy epinephelus lanceolatus is used as male parent fish, and sexual mature male parent fish is obtained through natural sexual maturity; the ovulation time and the spermiation time are consistent; collecting egg liquid and seminal fluid, inseminating to obtain fertilized eggs, and performing embryo incubation and illumination regulation and control cultivation after opening to obtain hybrid spots; carrying out authenticity identification on the hybrid spots by utilizing a primer pair, and determining that the hybrid spots inherit genetic information of the female parent fish and the male parent fish; the primer pair is a sequence pair composed of SEQ ID NO. 1 and SEQ ID NO. 2, and a sequence pair composed of SEQ ID NO. 3 and SEQ ID NO. 4. The technical blank of crossbreeding by taking the epinephelus trifoliatus as the parent epinephelus is filled, and the authenticity of the hybridized spots is reliable.

The invention discloses enterobacter mori capable of resisting fish aeromonas and application of the enterobacter mori, the bacterial strain is classified and named as enterobacter mori SCH0241, the enterobacter mori SCH0241 is preserved in the China Center for Type Culture Collection on October 17, 2024, and the preservation number is CCTCC NO: M 20242236. The enterobacter mori SCH0241 disclosed by the invention is derived from the intestinal tract of high-stress-resistance healthy hybrid crucian carassius auratus No. 2, has good contact dependence antibacterial activity on aeromonas veronii, and can improve the capability of resisting aeromonas veronii infection of zebra fish. Through construction of an insertion mutation library and phenotype screening, it is found that a gene cluster of SCH0241, which exerts contact inhibition toxicity, is T6SS-2. The enterobacter mori SCH0241 disclosed by the invention provides a new powerful tool for biological prevention and treatment of fish diseases caused by aeromonas veronii.

The invention relates to the field of distant hybridization breeding, and discloses an artificial breeding method for hybrid species of humpback perches and epinephelus fuscoguttatus, which comprises the following steps: selecting healthy humpback perches as female parent fishes, and screening healthy epinephelus fuscoguttatus as male parent fishes; carrying out intensive cultivation on the parents by means of nutrient enrichment and temperature regulation and control, and promoting synchronous development and maturation of gonads of the parents; after the parents reach the breeding state, eggs of the female parent fishes and seminal fluid of the male parent fishes are collected respectively, fertilized eggs are obtained through artificial insemination, incubation management such as temperature control and continuous oxygenation is conducted on the fertilized eggs, and finally hybrid grouper fries are obtained. The crossbreeding application of the humpback perch as the female parent and the epinephelus fuscoguttatus is realized, and the problems that in the traditional hybridization process, parent gonad development is asynchronous, and filial generations cannot develop are effectively solved; the bred hybrid provides technical guarantee for standardization and precision promotion of grouper crossbreeding, and creates favorable conditions for breeding new hybrid lines with excellent characters of parents.

The present invention relates to methods of breeding with spotted watermelon, Citrullus lanatus, lines and varieties. More specifically, methods of making homozygous-spotted diploid watermelon lines (HSDL) for use in breeding and methods of making homozygous-spotted tetraploid watermelon lines (HSDL) for use in breeding are described. Further, methods of producing 33%, 66%, or 100% spotted triploid hybrid watermelon plants, as well as methods of producing 50% or 100% spotted diploid hybrid watermelon plants, are described. This invention further relates to markers able to determine the percentage of spotted allele present in spotted watermelon lines and varieties. In addition, this invention relates to new and distinctive lines and varieties of watermelon with a spotted phenotype.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker for early identification of the cold resistance of an actinidia chinensis tree body in the dormancy period, and the SNP molecular marker is located at the Chr69297479 position on the No.6 chromosome of the actinidia chinensis and is closely associated with the cold-resistant phenotype median lethal temperature (LT50) of the actinidia chinensis. The method can be used for identifying the tree body dormancy period cold resistance of natural population and hybrid filial generation of actinidia chinensis. The homozygous genotype (CC) of the SNP locus corresponds to a high-cold-resistance individual in the dormancy period of an actinidia chinensis tree body, and the heterozygous genotype (TC) of the SNP locus corresponds to a low-cold-resistance individual. The cold resistance prediction accuracy in the F1-generation hybrid population reaches 94.94%, and the prediction accuracy in the natural population reaches 96.15%. The molecular marker detection method disclosed by the invention is convenient and rapid, has good specificity and high accuracy, is not influenced by climate, environment and the like, can rapidly and effectively evaluate the cold resistance of natural resources and hybrid filial generations of the actinidia chinensis, and provides powerful technical support for breeding cold-resistant varieties of the actinidia chinensis.

The invention discloses a gene of a rice indica-japonica intersubspecific hybrid sterile locus HS12 and application thereof, and belongs to the field of plant genetic engineering. The locus is positioned at the tail end of a long arm of a chromosome 12, is positioned between molecular markers Sy55 and Sy8, and comprises four fertility related genes iTx1, iTx2, iAd1 and jAd2. By functional knockout or inhibition of expression of the gene iTx1 and / or iTx2, iAd1 and / or jAd2 are / is transferred, a wide-compatibility rice material can be created, meanwhile, a molecular marker for detecting HS12 is further provided and used for filial generation detection and auxiliary breeding, indica-japonica hybrid sterility can be effectively overcome, and the setting rate of offspring is increased.

Inbred corn line, designated KW4FP1932, is disclosed. The disclosure relates to the seeds of inbred corn line KW4FP1932, to the plants and plant parts of inbred corn line KW4FP1932 and to methods for producing a corn plant, either inbred or hybrid, by crossing inbred corn line KW4FP1932 with itself or another corn line. The disclosure also relates to products produced from the seeds, plants, or parts thereof, of inbred corn line KW4FP1932 and / or of the hybrids produced using the inbred as a parent. The disclosure further relates to methods for producing a corn plant containing in its genetic material one or more transgenes and to the transgenic plants produced by that method and to methods for producing other corn lines derived from inbred corn line KW4FP1932.

The invention belongs to the technical field of biology, and discloses a method for screening plant interaction protein by combining big data with artificial intelligence and verifying protein interaction by using yeast two-hybrid. According to the method, AI is combined with big data, and co-expression protein of family protein is obtained through big data screening and analysis; aI interaction prediction and molecular docking are carried out on the proteins, and interaction information is further analyzed and extracted. And further analyzing the condition of the screened interaction protein by using a yeast two-hybridization or fluorescence co-localization experiment. According to the method, aiming at the characteristics of complex genomes and abundant sequencing data of species such as wheat, public sequencing data is creatively utilized to carry out an interaction protein screening process of subgenomes. The problems of long protein screening period, high cost, large workload, false positive protein screening, no co-expression and the like are solved. The interaction relationship between important gene families can be quickly and conveniently obtained, the interaction structural domain can be accurately and clearly found, and technical support is provided for research on analysis of a wheat key gene functional mechanism.

The invention provides seeds and plants of spinach hybrid SVVB2741. The invention thus relates to the plants, seeds, plant parts, and tissue cultures of spinach hybrid SVVB2741 and to methods for producing a spinach plant produced by crossing such plants with themselves or with another plant, such as a spinach plant of another genotype. The invention further relates to seeds and plants produced by such crossing. The invention further relates to plants, seeds, plant parts, and tissue cultures of spinach hybrid SVVB2741 comprising introduced beneficial or desirable traits.

The invention provides seeds and plants of pepper hybrid SVPH8928, pepper line SLR-8T20-6755, and pepper line SLR-8T18-6570. The invention thus relates to the plants, seeds, plant parts, and tissue cultures of pepper hybrid SVPH8928, pepper line SLR-8T20-6755, and pepper line SLR-8T18-6570 and to methods for producing a pepper plant produced by crossing such plants with themselves or with another plant, such as a pepper plant of another genotype. The invention further relates to seeds and plants produced by such crossing. The invention further relates to plants, seeds, plant parts, and tissue cultures of pepper hybrid pepper hybrid SVPH8928, pepper line SLR-8T20-6755, and pepper line SLR-8T18-6570 comprising introduced beneficial or desirable traits.

The invention relates to an artificial cross breeding method of Jinlong hybrid spot new germplasm, which belongs to the technical field of aquatic product distant cross breeding, and comprises the following steps: (1) parent breeding; (2) collecting and storing sperms of epinephelus lanceolatus; (3) carrying out reproductive regulation and ovum acquisition on the epinephelus coioides; (4) artificial preparation and incubation of hybrid fertilized eggs; according to the method, the artificial cross breeding of the female epinephelus coioides and the male epinephelus lanceolatus is realized for the first time, and the new germplasm Jinlong hybrid spots of the epinephelus coioides, which grow fast and are high in survival rate, are cultivated. The body weight of the Jinlong hybrid Epinephelus coioides of 3 months old is increased by 46.45% compared with that of pure Epinephelus coioides; and the survival rate of the new germplasm is increased by 62.50% compared with that of the epinephelus coioides. The industrial problems that artificial spawning of large groupers is difficult, the survival rate of fries is low, and fast-growing new germplasm lacks are effectively solved.

The invention discloses a grain-saving disease-resistant high-yield black feather laying hen commercial line male parent breeding method and a commercial line seed production method, which comprises the following specific steps: step S1: by taking a homozygous dwarf gene (dw) type dark purple fast-feathering chicken as a basic material, hybridizing with a princess chicken to obtain a first generation (F1) hybridized synthetic line chick; s2, the bred and selected first-generation sibs are subjected to inbreeding, and second-generation (F2) chicks are generated; s3, selecting cocks with excellent quality traits of the second generation and backcrossing the cocks with the hens of the first generation to generate a third generation (F3); s4, screening out cocks conforming to target traits from the third generation (F3), namely the grain-saving disease-resistant high-yield black feather laying hen commercial line male parents; then selecting a Rhode Island Red parent generation hen (slow feathering) as a female parent to form a grain-saving disease-resistant high-yield black feather laying hen commercial line; and the bred commercial generation chicks can sex automatically.

The invention belongs to the field of crop genetic breeding, and relates to a method for efficiently creating brown glume rice and application thereof. According to the application, a stably inherited brown glume mutant hcm is created through radiation mutagenesis, and a molecular marker of brown glume characters is developed; a restorer line Huazhan is used as a recurrent parent for recurrent breeding with a mutant hcm, and the brown glume rice is efficiently created by combining molecular marker-assisted selection. And matching the rice sterile line Yunnan 165S with brown glume rice to obtain F1 hybrids, and planting the F1 hybrids to obtain brown glume hybrid rice grains. According to the invention, the brown character of the hcm glume of the mutant is directionally introduced into the restoring line Huazhan and the hybrid thereof, and when the improved restoring line is used for carrying out hybrid rice seed production, the hybrid and the selfing seed of the restoring line can be distinguished by utilizing the color difference of the glume of the male and female parent seeds, so that the large-scale mixed planting, mixed harvesting and color sorting separation of the hybrid are realized; the seed production efficiency of the hybrid rice is favorably improved.

The invention relates to a Canola hybrid designated 22CN0172, essentially derived variants of that Canola hybrid, to the cells, seeds, plants, and plant parts of this Canola hybrid 22CN0172. The invention also relates to methods for producing a canola plant containing in its genetic material one or more traits introgressed into 22CN0172 through backcross conversion and / or transformation, and to the Canola seed, plant and plant part produced thereby. The invention also relates to uses of Canola hybrid 22CN0172.

A novel maize variety designated X09T080 and seed, plants and plant parts thereof are produced by crossing inbred maize varieties. Methods for producing a maize plant by crossing hybrid maize variety X09T080 with another maize plant are disclosed. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into X09T080 through backcrossing or genetic transformation, and to the maize seed, plant and plant part produced thereby are described. Maize variety X09T080, the seed, the plant produced from the seed, and variants, mutants, and minor modifications of maize variety X09T080 are provided. Methods for producing maize varieties derived from maize variety X09T080 and methods of using maize variety X09T080 are disclosed.

A novel maize variety designated X05T063 and seed, plants and plant parts thereof are produced by crossing inbred maize varieties. Methods for producing a maize plant by crossing hybrid maize variety X05T063 with another maize plant are disclosed. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into X05T063 through backcrossing or genetic transformation, and to the maize seed, plant and plant part produced thereby are described. Maize variety X05T063, the seed, the plant produced from the seed, and variants, mutants, and minor modifications of maize variety X05T063 are provided. Methods for producing maize varieties derived from maize variety X05T063 and methods of using maize variety X05T063 are disclosed.

A novel canola variety designated 4PPVF68A and seed, plants and plant parts thereof. Methods for producing a canola plant that comprise crossing canola variety 4PPVF68A with another canola plant. Methods for producing a canola plant containing in its genetic material one or more traits introgressed into 4PPVF68A through backcross conversion and / or transformation, and to the canola seed, plant and plant part produced thereby. Hybrid canola seed, plant or plant part produced by crossing the canola variety 4PPVF68A or a locus conversion of 4PPVF68A with another canola variety.

A novel maize variety designated X09T084 and seed, plants and plant parts thereof are produced by crossing inbred maize varieties. Methods for producing a maize plant by crossing hybrid maize variety X09T084 with another maize plant are disclosed. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into X09T084 through backcrossing or genetic transformation, and to the maize seed, plant and plant part produced thereby are described. Maize variety X09T084, the seed, the plant produced from the seed, and variants, mutants, and minor modifications of maize variety X09T084 are provided. Methods for producing maize varieties derived from maize variety X09T084 and methods of using maize variety X09T084 are disclosed.

New and unique brown varieties of the mushroom Agaricus bisporus Imbach were produced by crossing the progeny of brown mushrooms and off-white hybrid mushrooms to bridging cross strains. The resultant hybrids, named 'RO10425', 'RO14176', and'RO12756', exhibit distinct and stable characteristics. Hybrid 'RO10425' exhibits a lighter cap color, thicker cap flesh, and rounder cap, especially when compared to available brown mushroom varieties. 'RO 10425' also exhibits fast mushroom development and high yield. Hybrid 'RO14176' exhibits a light cap color, thicker cap flesh, rounder cap, faster mushroom development, and higher yield, especially when compared to available brown mushroom varieties. Hybrid 'RO12756' exhibits a light cap color, thicker cap flesh, rounder cap, faster mushroom development, and high yield, especially when compared to available brown mushroom varieties.

The invention discloses a bactrocera dorsalis point mutation gene editing method based on CRISPR-Cas9 and homologous directional repair, and relates to the technical field of gene editing. The method comprises the following steps: designing high-specificity sgRNA for a PAM sequence adjacent region of a target gene; extending the length of the homologous arm to 100bp, and designing a single-stranded DNA (deoxyribonucleic acid) containing a mutation site as a homologous repair template; after a mixture containing the high-specificity sgRNA, the homologous repair template and Cas9 protein is injected into a bactrocera dorsalis fertilized egg in a microinjection mode, incubation and incubation are carried out, and G0-generation imagoes are obtained through culture; hybridizing the G0-generation imago with wild bactrocera dorsalis, and screening to obtain a G1-generation hybrid mutant; and carrying out selfing by using the G1-generation heterozygous mutant, and screening to obtain a G2-generation homozygous mutant. By adopting the method, the mutation efficiency and embryo survival rate of bactrocera dorsalis can be effectively improved.

A novel maize variety designated X08T074 and seed, plants and plant parts thereof are produced by crossing inbred maize varieties. Methods for producing a maize plant by crossing hybrid maize variety X08T074 with another maize plant are disclosed. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into X08T074 through backcrossing or genetic transformation, and to the maize seed, plant and plant part produced thereby are described. Maize variety X08T074, the seed, the plant produced from the seed, and variants, mutants, and minor modifications of maize variety X08T074 are provided. Methods for producing maize varieties derived from maize variety X08T074 and methods of using maize variety X08T074 are disclosed.

A novel maize variety designated X13T112 and seed, plants and plant parts thereof are produced by crossing inbred maize varieties. Methods for producing a maize plant by crossing hybrid maize variety X13T112 with another maize plant are disclosed. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into X13T112 through backcrossing or genetic transformation, and to the maize seed, plant and plant part produced thereby are described. Maize variety X13T112, the seed, the plant produced from the seed, and variants, mutants, and minor modifications of maize variety X13T112 are provided. Methods for producing maize varieties derived from maize variety X13T112 and methods of using maize variety X13T112 are disclosed.

A novel maize variety designated X18T233 and seed, plants and plant parts thereof are produced by crossing inbred maize varieties. Methods for producing a maize plant by crossing hybrid maize variety X18T233 with another maize plant are disclosed. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into X18T233 through backcrossing or genetic transformation, and to the maize seed, plant and plant part produced thereby are described. Maize variety X18T233, the seed, the plant produced from the seed, and variants, mutants, and minor modifications of maize variety X18T233 are provided. Methods for producing maize varieties derived from maize variety X18T233 and methods of using maize variety X18T233 are disclosed.

A novel maize variety designated X87T010 and seed, plants and plant parts thereof are produced by crossing inbred maize varieties. Methods for producing a maize plant by crossing hybrid maize variety X87T010 with another maize plant are disclosed. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into X87T010 through backcrossing or genetic transformation, and to the maize seed, plant and plant part produced thereby are described. Maize variety X87T010, the seed, the plant produced from the seed, and variants, mutants, and minor modifications of maize variety X87T010 are provided. Methods for producing maize varieties derived from maize variety X87T010 and methods of using maize variety X87T010 are disclosed.

A new and distinct hybrid of Heuchera plant named ‘Sultry Night’ has a medium-sized habit with foliage that is moderately-dissected, moderately-wavy, lightly overlapping cordate bases, light marginal undulation, glossy leaf blades of dark purplish-grey with a lighter purplish-red overlay on short petioles. The leaf blades are shallowly dissected at five main lobes with many smaller indentations. The new plant has upright, moderately-dense, branched panicles producing pinkish flower effect. The new plant is useful in the landscape or in containers.

A maize variety designated X13R112 and seed, plants and plant parts thereof are produced by crossing inbred maize varieties. Methods for producing a maize plant by crossing hybrid maize variety X13R112 with another maize plant are disclosed. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into X13R112 through backcrossing or genetic transformation, and to the maize seed, plant and plant part produced thereby are described. Maize variety X13R112, the seed, the plant produced from the seed, and variants, mutants, and minor modifications of maize variety X13R112 are provided. Methods for producing maize varieties derived from maize variety X13R112 and methods of using maize variety X13R112 are disclosed.