1242 results about "Hybrid" patented technology

The invention provides a functional molecular marker for rice anti-blast gene Pi9 and application thereof, belonging to the field of crop molecular genetic breeding science. The invention finds out that the rice anti-blast gene Pi9 has a 10bp insertion/deletion site positioned between the gene promoter -516 and -517, wherein the nucleotide sequence is disclosed as SEQ ID NO.1; and on such basis, the invention develops a method of the functional molecular marker for gene Pi9. By detecting the functional molecular marker, the invention can accurately detect whether genomes of different rice species contain the Pi9 functional gene and detect the homozygotic state, and can be used for screening rice hybrid transformed descendant plants to enhance the breeding efficiency of the rice anti-blast material, thereby effectively controlling the size of the breeding population, obviously saving the breeding and screening cost and obtaining the anti-blast rice species containing the Pi9 functional gene.

The invention discloses a Dendrobium in vitro crossbreeding method. The method can be used to greatly shorten the maturation period of fruits, to enable a hybrid to bloom in vitro in a short period so as to observe flower shapes and colors, and to cultivate a novel variety, thus accelerating Dendrobium breeding, the first such report internationally. Since in vitro Dendrobium blooms annually, it enables crossbreeding of varieties having different inflorescences in nature. In addition, the medium used at each stage of the invention utilizes Hyponex which has a unique composition and costs little, thus allowing for a high blossoming rate and rapid fruit development. Also, only simple plant tissue culture equipment is required for implementing the invention, thus the entire breeding method is simple and low cost, and provides conditions for cultivating of high-quality Dendrobium varieties.

The invention provides a formula of a culture medium for white beech mushroom and a method for cultivating white beech mushroom, relates to an edible fungus cultivating technique, and mainly relates to a culture medium and a cultivating method for white beech mushroom. The formula of the culture medium comprises the following components: 28.5%-72.5% of hybrid sawdust, 10%-20% of cottonseed hulls, 10%-20% of corn cobs, 4%-8% of wheat, 1%-5% of beanstalks, 2%-12% of rice bran, 0.25%-0.75% of lime, 0.25%-0.75% of plaster and 1%-5 of soybean hulls. The cultivating method comprises the steps that: the hybrid sawdust is decayed by a decay accelerator and then evenly mixed with the other components, then the mixture is bottled, sterilized, inoculated and the like; and finally, fruiting management is performed. The method provided by the invention has the advantages that the culture medium is treated in advance; a full-automatic bionic system is employed for the key link; the whole cultivation process is free of pollution and fungus emerging is quick; the hypha are good in quality and grow densely, and the growing power of the hypha is strong; the produced white beech mushroom is pure white and tender, and good in quality; the production cycle is only 100-120 days from inoculation to harvesting; the biological efficiency reaches 85-90%; and the competitiveness of the white beech mushroom product is enhanced.

The invention discloses an SSR molecular marker A002 for identifying males and females of kiwi fruit hybrid populations, relating to the technical field of molecular genetic breeding. The male plant specific fragment nucleotide sequence of the molecular marker A002 is shown in SEQ.ID No.1; the female plant specific fragment nucleotide sequence is shown in SEQ.ID No.2; and the primer sequence is shown in SEQ.ID No.3 and SEQ.ID No.4. The molecular marker A002 can be used for the early identification detection of males and females of kiwi fruits so as to avoid the great waste caused by abundant male plants in the breeding and production; by utilizing a high-density genetic map, sex related molecular markers can be rapidly explored to assist the breeding and important practical experience is provided for the development of the functional markers; and being used for marker assisted selection of males and females, the molecular marker A002 is capable of greatly improving the selection efficiency of the female plants in the breeding and shortening the breeding period, and has great application potential and relatively high economic value.

The invention belongs to the field of crop biotechnology and in particular relates to a molecular marker of a rice-blast-resistant gene Pi2 and application of the molecular marker. The upstream and downstream molecular markers are based on specific insertion/deletion loci polymorphism of nucleotide sequences shown in SEQ ID NO.1 and SEQ ID NO.2. The two molecular markers are co-dominant molecular markers developed for close linkage regions on two sides of the functional gene Pi2, and have the advantages of being high in accuracy and capable of being widely applied to different breeding parents. By detecting the two molecular markers, whether a detected rice material has the rice-blast-resistant gene Pi2 or not can be accurately judged. By virtue of a molecular marker combination, whether a hybrid transferred progeny plant genome of a parent of the functional gene Pi2 and other parents has the functional gene Pi2 or not and the homozygous state of the functional gene Pi2 can be accurately detected, and the breeding efficiency of a rice-blast-resistant rice variety with the functional gene Pi2 is improved.

The invention discloses a bee attractant and a method for field seed production and pollination of soybean hybrids. The method comprises the following steps of: selecting a field which is suitable for the growth of soybeans, duly sowing the soybean hybrids, and intercropping male parents and female parents across ridges; selecting a required amount of healthy pollination swarm within 5 to 7 days before pollination, and transporting into the field of the soybeans at the early flowering stage of the soybeans; feeding attractant syrup within 1 hour before the bee attractant is sprayed everyday or every other day in the seed production field of the soybean hybrids which just blossom; spraying the bee attractant on leaves of hybrid soybean plants of the male parents and the female parents in 1 to 5 ridges of field across 1 to 5 ridges of field every 1 to 3 days so as to ensure that bees are attracted into the field and fall down to the leaves of the hybrid soybean plants to eat the syrup; spraying the attractant on two sides of stalks of the hybrid soybean plants to attract the bees to fall down to hybrid soybeans to collect so as to pollinate the hybrid soybeans; and stopping spraying the bee attractant in rainy days, and restoring the spray when the weather gets better so as to maintain the attractant which can meet the requirements in the field. The attractant is obtained by mixing a honeycomb liquid, a flower aroma imitation liquid and syrup in a proportion and diluting by using quantitative cold boiled water.

Provided by this invention are novel intergeneric bivalve shellfish hybrids, including clams and scallops. Also provided are methods for producing the novel hybrids and their progeny.

The invention provides seed and plants of cucumber hybrid SV1096CN and the parent lines thereof. The invention thus relates to the plants, seeds and tissue cultures of cucumber hybrid SV1096CN and the parent lines thereof, and to methods for producing a cucumber plant produced by crossing such plants with themselves or with another cucumber plant, such as a plant of another genotype. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of such plants, including the fruit and gametes of such plants.

The invention discloses a method for breeding Wannan boars, which includes: subjecting paternal Duroc pigs and maternal Wannan spotted pigs to hybridization to obtain Du-Wan two-way hybrid sows; subjecting pure Wannan wide boars as male parents and the Du-Wan two-way hybrid sows as female parents to hybridization to obtain wild boars 50% of Wannan wild boars; subjecting the pure Wannan male wild boars as male parents and the boars 50% of the Wannan wild sows as female parents to hybridization to obtain wild boars 12.5% of Du-Wan blood and 75% of Wannan wild boars; and finally subjecting the wild boars which are 12.5% of Du-Wan blood and 75% of the Wannan wild boars as male parents and the wild boars 50% of the Wannnan wild boars as female parents to hybridization so as to obtain wild boars 12.5% of Du-Wan blood. By hybridizing the Wannan wild boars, the Wannan spotted pigs and Duroc pigs, the obtained wild boars are stable in hereditary character, high in adaptability, resistant to coarse feed, low in feeding quantity, low in rearing cost, fine in reproductive performance, high in disease resistance, high in meat nutrition value, full of wild taste, high in slaughter rate and good in economic benefit.

The invention relates to a slaughter type high-quality chilling fresh chicken breeding method. The breeding method includes: selecting recessive white-feather chickens (B line) having green shins and yellow skins as first male parents, and selecting large cockscomb high yield chickens (C line) having yellow shins and yellow skins as first female parents; hybridizing the B line and the C line to acquire parental generations (BC system) to be as final female parents according to the heredity principle that a green shin gene is sex-linked and an autosome controls a skin color gene, wherein parental chickens can be distinguished through the shin color, the shins of the female chickens are green, and the shins of the male chickens are yellow. using yellow-feather fast-growing chickens (A line) having green shins, yellow skins, and large cockscombs as final male parents, producing commodity chickens (ABC) by A line male X (B line male X C line female) female mating hybrid, wherein commodity male and female chickens have green shins, yellow skins, large cockscombs, and wide chests. The commodity chickens can be sold at the age of 63-65 days, the average weight of the female chickens is 1.6-1.65 Kg, the average weight of the male chickens is 1.8-1.85 Kg, the average uniformity can reach more than 96%; the dressing percentage, the whole net carcass rate, and the chest muscle rate can exceed 85%, 65%, and 18%, and the carcass character has significant advantages.

The invention discloses a breeding method for the improved variety of fishes. The breeding method comprises the following steps of: a. selection of ripe fishes; b. parental hybrid; c. parental mark; d. family construction; and e. improved variety breeding. By adopting the technical scheme, the family for the bred largemouth black bass is established, the families of the largemouth black bass are mated and novel variety of the largemouth black bass which has high quality, high yield and stable yield is suitable for the breeding environment in China is bred.