4500results about "Plant genotype modification" patented technology

A Chinese cabbage hybrid seed production marking method relates to the technical field of Chinese cabbage hybrid seed production. The technical scheme of the method is as follows: after Chinese cabbage hybrid seed production pollination is finished, firstly holding the pedicel of a pollinated flower with the left hand; then transversely tearing off two sepals from the root with the right hand, wherein light movement is required. The Chinese cabbage hybrid seed production marking method can perform marking without rope tying, ring arranging or stamp-pad ink coating, and is low in cost and high in accuracy.

The present disclosure provides drug responsive domains derived from human carbonic anhydrase 2 that can modulate protein stability for human interleukin 15 (IL15) payloads, as well as compositions and methods of use thereof.

This invention belongs to the field of biopharmaceuticals and provides a keratin YK93-6, its encoding nucleic acid molecule, an expression vector containing the nucleic acid molecule, a host cell containing the expression vector or whose genome integrates the nucleic acid molecule, as well as a preparation method and a pharmaceutical composition thereof. It also provides the application of the above-mentioned keratin YK93-6 and other products in the preparation of drugs for treating benign prostatic hyperplasia, lymphoma, melanoma, analgesia, lactation, breast cancer, lung cancer, uterine fibroids, coagulation, etc.

This invention relates to the field of plant genetic engineering technology, and more particularly to... SsNHX1 and SsSOS2 Dual-gene plant expression vector and its application in improving salt tolerance in alfalfa. The plant expression vector uses pCAMBIA1300 as its backbone and inserts genes derived from Suaeda salsa. SsNHX1 Genes and SsSOS2 It is constructed from genes. SsNHX1 Genes and SsSOS2 The CDS coding sequence of the gene is shown in SEQ ID NO:1 and SEQ ID NO:2 of the sequence listing. Using Agrobacterium tumefaciens EHA105-mediated transformation, the vector was introduced into the leaves of sterile alfalfa seedlings. Positive transgenic plants were obtained through co-culture, callus induction differentiation, and PCR identification. The advantage lies in the synergistic effect of the two genes, which effectively enhances the salt tolerance of alfalfa, enabling it to grow normally under 200–300 mM NaCl stress, providing a new solution for forage cultivation in saline soils.

This invention discloses a method for constructing a biological model of tertiary lymphoid structures, which can rapidly and efficiently induce the generation of in situ TLS, without relying on specific tissues or organs, and without the need for complex and expensive preparation processes such as cell editing and transgenic mice. It can generate stable, mature, and long-lasting TLS structures within 2 to 3 weeks, and has the characteristics of short cycle, low cost, simple operation, reproducibility, safety and effectiveness.

This invention relates to the field of plant genetic engineering technology, and more particularly to... SsNHX1 and SsSOS1 A dual-gene plant expression vector and its application in improving salt tolerance in alfalfa. The plant expression vector uses pCAMBIA3301 as its backbone and inserts genes derived from Suaeda salsa. SsNHX1 Genes and SsSOS1 It is constructed from genes. SsNHX1 Genes and SsSOS1 The nucleotide sequences of the gene are shown in SEQ ID NO:1 and SEQ ID NO:2. Using Agrobacterium tumefaciens EHA105-mediated transformation, the vector was introduced into the leaves of sterile alfalfa seedlings. Positive transgenic plants were obtained through co-culture, callus induction differentiation, and PCR identification. The advantages are: the synergistic effect of the two genes effectively enhances the salt tolerance of alfalfa, enabling it to grow normally under 200–300 mM NaCl stress, providing a new solution for forage cultivation in saline soils.

This invention belongs to the field of biopharmaceuticals and provides a keratin YK93-8, its encoding nucleic acid molecule, an expression vector containing the nucleic acid molecule, a host cell containing the expression vector or whose genome integrates the nucleic acid molecule, as well as a preparation method and a pharmaceutical composition thereof. It also provides the application of the above-mentioned keratin YK93-8 and other products in the preparation of drugs for treating benign prostatic hyperplasia, lymphoma, melanoma, breast cancer, lung cancer, analgesia, lactation, uterine fibroids, coagulation, etc.

Methods are disclosed for producing high-titer recombinant adeno-associated virus (rAAV) using a modified adenovirus-based helper nucleic acid. One aspect provided herein is a human adenovirus 5 (hAd)-based nucleic acid that does not contain one or more of (a) an E4 region having E4-ORF6 / 7, (b) a viral associated (VA) RNA region, (c) an E2A region having L4-22K and L4-33K, (d) at least one packaging protein, (e) at least one structural protein, (f) a major late promoter (MLP), (g) an E1 region, and / or (h) an E3 region.

The present invention belongs to the field of plant genetics research, and particularly relates to gene functions for increasing plant biomass and seed yield. The present invention discloses the use of a gene that negatively regulates plant biomass and seed yield. This gene is the AT3G28990 gene whose nucleotide sequence is shown in SEQ ID NO: 1. By knocking out the AT3G28990 gene in plants, the biomass and seed yield of plants can be increased. Knocking out the AT3G28990 gene of Arabidopsis thaliana can effectively increase the biomass and yield of plants. This has important reference significance for the breeding of high-yield crops.

Provided is a bispecific antibody and use thereof, the bispecific antibody comprising: a first domain that specifically binds the interleukin-6 receptor IL-6R, and a second domain that specifically binds interleukin-23. The bispecific antibody can simultaneously target IL-6R and IL-23p19, with stronger specificity, more accurately targeting tumor cells and reducing the adverse effects of off-target toxicity and can perform a unique function to play a biological function that is difficult to achieve with monoclonal antibody drugs. Compared with monoclonal antibody combination therapies, it can also effectively reduce the cost of treatment and break the current upper limit of therapeutic efficacy in the treatment of autoimmune diseases such as rheumatoid arthritis and IBD.

The application discloses a florfenicol and thiamphenicol monoclonal antibody and application thereof, and belongs to the technical field of biology. The monoclonal antibody contains a heavy chain variable region and a light chain variable region, the heavy chain variable region and the light chain variable region are both composed of a complementarity determining region and a framework region, and the complementarity determining region is composed of CDR1, CDR2 and CDR3. The monoclonal antibody can be used for preparing a florfenicol and thiamphenicol detection product. The florfenicol and thiamphenicol colloidal gold detection test strip provided by the application has the characteristics of high sensitivity, good specificity and strong stability.

The application discloses a kind of Candida albicans BUB2 Gene deletion attenuated strain, its construction method and application belong to the field of genetic engineering technology.The attenuated strain is Candida albicans BUB2 deletion strain with BUB2 gene not expressed, its construction method uses CRISPR / Cas9 system, with HIS1 as screening marker, by knocking out box and sgRNA, Cas9 co-transforms Candida albicans wild type strain, replaces two alleles of BUB2 gene to obtain.The phenotype experiment verifies that BUB2 deletion strain is highly sensitive to hydrogen peroxide, cadmium chloride and congo red;Hyphal induction experiment shows that the hyphal production capacity of deletion strain significantly decreases, and the hyphal length significantly shortens;Biofilm formation experiment shows that the biofilm formation capacity of deletion strain is about 41.4% of wild type;Galleria mellonella and mouse infection model confirm that the virulence of BUB2 deletion strain significantly decreases, and the survival rate of infection group is much higher than that of wild type group.The application provides a new action target for clinical treatment of Candida albicans infection, and can be used for preparing or screening anti-Candida albicans infection drugs, and has good application prospect.

The application belongs to the technical field of biology and particularly relates to an African swine fever virus pNP868R protein monoclonal antibody and a preparation method and application thereof. The application takes the pNP868R protein prepared by eukaryotic expression as an antigen, immunizes BALB / c mice, uses hybridoma technology, and obtains a hybridoma cell line capable of stably secreting the anti-African swine fever virus pNP868R protein monoclonal antibody through cell fusion, indirect ELISA method screening, limited dilution method subcloning and hybridoma continuous passage culture. The application also obtains an African swine fever virus pNP868R protein monoclonal antibody. The monoclonal antibody has high affinity with the pNP868R protein and can be applied in Western-Blot experiments, indirect immunofluorescence experiments, immunoprecipitation experiments and the capture of proteins interacting with pNP868R, and can be used for the diagnosis, prevention or treatment of African swine fever.

The present invention provides an hBCMA-binding polypeptide comprising at least one motif that binds to hBCMA, wherein the peptide has the following structure: [N-terminal portion]-[Helix 1]-[Spacer portion]-[Helix 2]-[C-terminal portion], and the hBCMA-binding motif is the portion [Helix 1]-[Spacer portion]-[Helix 2]. The present invention further provides a pharmaceutical composition comprising the hBCMA-binding polypeptide, and the use of the hBCMA-binding polypeptide or the pharmaceutical composition for use as a medicament, particularly for the treatment or prevention of cancer.

The present application relates to a kind of aldehyde ketone reductase, and the gene of the reductase, using the enzyme of the present application can biosynthesize the important pharmacological active action of the four-ring alkaloid component, such as galanthamine or rivastigmine.

The present invention provides a method for the production of a recombinant adeno-associated viral vector (rAAV), comprising contacting a host cell with a solution comprising at least one compound of formula (I), (IA), (IB), (II), (III), or (IV), or a salt thereof, or vitamin B, or any combination thereof. Also provided is a method for increasing production of rAAV by a host cell, comprising contacting the host cell with a solution comprising at least one compound of formula (I), (IA), (IB), (II), (III), or (IV), or a salt thereof, or vitamin B, or any combination thereof.

The present disclosure provides methods and compositions for enhancing immune responses against cancer and pathogens. The subject matter of the present disclosure provides methods and compositions for enhancing immune responses against cancer and pathogens. The subject matter of the present disclosure relates to cells comprising a c-Kit mutant, for example, a c-Kit mutant comprising an activating mutation. The cells may further comprise an antigen-recognition receptor (e.g., a chimeric antigen receptor (CAR) or a T cell receptor (TCR)). The subject matter of the present disclosure relates to the use of the cells for treatment, for example, for the treatment of cancer.

Provided are: a genome-edited plant production method by which genome editing can be applied to plants including the family Leguminosae that is difficult to transform, without undergoing processes of tissue culture requiring transformation and time; and a genome-edited plant obtained by said method. The present invention pertains to a genome-edited plant production method and a genome-edited plant obtained by said method, the genome-edited plant production method comprising: providing a cutout in a part of a plant capable of autonomously growing; injecting, to the cutout, an Agrobacterium-containing solution for genome editing; introducing a mutation into the plant without leaving a foreign gene therein; and collecting seeds from the plant, into which the mutation has been introduced, without subjecting the plant to a re-differentiation step, to obtain a genome-edited plant.

The present disclosure provides a HER2 binder comprising a VHH domain that specifically binds to HER2. Also provided are nucleic acids encoding the protein, vectors and host cells comprising the nucleic acids, and methods of manufacturing and using the HER2 binder. The present disclosure also provides a HER2 binder conjugate in which the protein is conjugated to a detectable label, and a HER2-binding targeted radiotherapy agent for therapeutic and / or diagnostic purposes. Also provided are methods of using the HER2 binder for detecting, monitoring and / or treating diseases and conditions such as cancer.

The present invention relates to a method for evaluating or predicting a therapeutic response to treatment with an IL7R modulator, such as an IL7R antagonist or agonist, in a patient, and more particularly to the identification of biomarkers for evaluating or predicting whether an IL7R modulator is effective in treating a patient. The present invention also relates to a method for screening for a compound that is effective in treating a patient. The biomarkers are BCL2, CISH, SOCS2, FLT3LG, PTGER2, and DPP4.

The disclosure relates to novel soybean plants, plant parts, and nucleotide sequences in soybean plants comprising an amorphic allele of a RPF1 gene, along with methods of using and making the same.

To date, there is still a need in this field for more efficient cancer treatment. More specifically, there is still a lack of tools that can be used for more effective cancer immunotherapy. [Solution] The present invention relates to the use of human growth differentiation factor 15 (GDF-15) inhibitors in the treatment of solid tumors, and to the use of such inhibitors in combination with immune checkpoint blockers.

To provide methods of making immune effector cells (e.g., T cells, NK cells) that can be engineered to express a chimeric antigen receptor (CAR), compositions comprising the same and reaction mixtures.SOLUTION: A method for expanding and / or activating a population of immune cells (e.g., immune effector cells) includes introducing a nucleic acid encoding a first chimeric antigen receptor (CAR) molecule into an immune cell population, under conditions suitable for transient expression of the CAR molecule, thereby producing a first CAR-expressing cell population, where the CAR molecule comprises an antigen binding domain of an antigen molecule; and contacting the first CAR-expressing cell population with a ligand of the CAR antigen binding domain selected from a cognate antigen molecule (e.g., a recombinant antigen) or an anti-idiotypic antibody molecule, under conditions such that immune cell expansion and / or activation occurs, thereby producing an expanded and / or activated immune cell population.SELECTED DRAWING: None

The application relates to the field of genetic engineering, and discloses an Aspergillus niger, a construction method and application of the Aspergillus niger, and a method for producing citric acid and / or succinic acid. Aspergillus niger The Aspergillus niger is obtained by simultaneously overexpressing alpha-amylase and aspartic protease in a starting strain. The Aspergillus niger provided by the application can realize efficient degradation and conversion of starch by synergistically overexpressing amyA genes and pepA genes, solve the technical bottleneck that the original strain is limited in growth in a starch-based fermentation system and has insufficient acid production capacity, can efficiently produce succinic acid and citric acid by taking low-cost starch as the only carbon source, significantly reduce the industrial production cost of organic acid, and promote the green and sustainable upgrading of the fermentation industry.

The present invention relates to a combination of a specific binding molecule that binds to human ANXA1 and a second active agent for use in the treatment of cancer. The second active agent includes thymidylate synthase inhibitors, nucleobase analogs, checkpoint inhibitors, proteasome inhibitors, taxanes, platinum-based chemotherapy agents, and nucleoside analogs. Suitable cancers for treatment include pancreatic cancer, colon cancer, breast cancer, lung cancer, myeloma, and mantle cell lymphoma. Also provided are related kits, products, and uses.

The application belongs to the field of bioengineering and microbial fermentation technology, and discloses an engineered Aurantiochytrium limacinum with high palmitoleic acid yield and a construction method and application thereof. The engineered Aurantiochytrium limacinum is obtained by introducing and expressing a heterologous Δ9 desaturase gene in Aurantiochytrium limacinum through genetic engineering, and the heterologous Δ9 desaturase gene is subjected to gene source screening, promoter optimization and target gene copy number optimization to enhance the conversion ability of C16:0 to C16:1, thereby significantly improving the content of palmitoleic acid in Aurantiochytrium limacinum oil. The engineered strain can significantly improve the content of palmitoleic acid, preferably more than 4%, and further more than 6%. The technical route is clear and operable, and can provide an engineered strain and an application scheme for the industrialized production of a functional lipid raw material with high palmitoleic acid.

The application discloses a Campylobacter-specific protein antibody, an antibody-coupled magnetic bead and application. The antibody is a monoclonal antibody 1A10 and a monoclonal antibody 3D9, the monoclonal antibody 1A10 comprises a light chain variable region and a heavy chain variable region, the amino acid sequences of the light chain variable region and the heavy chain variable region of the monoclonal antibody 1A10 are shown in SEQ ID NO: 7 and 8 respectively; the monoclonal antibody 3D9 comprises a light chain variable region and a heavy chain variable region, the amino acid sequences of the light chain variable region and the heavy chain variable region of the monoclonal antibody 3D9 are shown in SEQ ID NO: 9 and 10 respectively. The antibody-coupled magnetic bead is coupled by the monoclonal antibody 1A10, the monoclonal antibody 3D9 and activated carboxyl magnetic beads respectively. The antibody-coupled magnetic bead can specifically enrich Campylobacter, has high enrichment efficiency, simple enrichment operation, can be applied to metabolic product analysis of Campylobacter, and can be used for qualitative and quantitative analysis of the Campylobacter.

This invention discloses the application of the NtMPK4 gene in tobacco cold tolerance, belonging to the field of molecular biology. The NtMPK4 gene sequence is shown in SEQ ID NO.1. The NtMPK4 gene negatively regulates tobacco cold tolerance. The discovery and functional identification of this gene provide important genetic resources and a theoretical basis for low-temperature tolerance breeding of tobacco.

The present application provides an engineered circular RNA capable of recruiting adenosine deaminase acting on RNA (ADAR; arRNA) and its application in treating Duchenne muscular dystrophy (DMD).

This utility model provides a rapeseed pollination isolation device, belonging to the field of rapeseed pollination isolation. It includes four sets of isolation bags and straps attached to the isolation bags. It also includes: a support rod with a height adjustment mechanism on its outer wall, capable of raising and lowering the isolation bags; a telescopic mechanism located on the side wall of the height adjustment mechanism, capable of adjusting the position of the isolation bags; and a rotating mechanism located on the telescopic mechanism, capable of adjusting the tilt angle of the isolation bags. This allows it to adapt to rapeseed in different conditions. By setting a height adjustment mechanism to adjust the height of the isolation bags, a telescopic mechanism to adjust the position of the isolation bags, and a rotating mechanism to adjust the tilt angle of the isolation bags, this utility model achieves precise three-way positioning, is compatible with lodged and tall / short plants, and improves the effectiveness of the isolation bagging.