25021 results about "Bioinformatics" patented technology

The invention relates to the technical field of medical artificial intelligence and knowledge graph construction, in particular to a rare disease knowledge graph construction method based on modal injection and multi-modal fusion. Comprising the following steps: S1, collecting multi-modal medical information including texts, images and genes; s2, standardization processing is carried out, and a three-layer metadata structure is constructed; s3, complementing missing modal data, and performing feature extraction and unified dimension conversion on the modal data to realize representation alignment in a shared semantic space; s4, performing multi-level semantic fusion to obtain a unified fusion semantic vector; and S5, constructing a double-layer structure system rare disease knowledge graph comprising an ontology layer and an instance layer. According to the method, multi-modal medical information of texts, images and genes is selected to construct the knowledge graph of the rare disease, the application range, coverage and accuracy of the knowledge graph are improved, correspondence adaptation of rare cases during clinical diagnosis and treatment of the rare disease can be achieved, and the method has high recognition capacity.

The method can include: recording a neural state, determining a latent array, and determining an output based on the latent array. In variants, the method can function to enable a subject to use neural signals to navigate within a high-dimensional concept space to control an output (e.g., image generation, motor control, communication, etc.).

A system and method for maintaining an oxygen concentration of a biological sample. The oxygen concentration can be maintained by measuring the oxygen concentration within the biological sample and adjusting a rate of an oxygen supplier in response to this measurement. For example, when the oxygen concentration is below a threshold, oxygen can be delivered to the biological sample at a higher rate.

Provided herein are methods of characterizing tumors or a region of interest in a biological sample.

The invention discloses an intestinal cancer immune response graph neural network prediction method, a medium and equipment, and the method comprises the steps: collecting pathological image information, immunodetection information and basic clinical information, extracting a tissue space distribution characteristic spectrum through a deep convolutional network, and constructing a graph neural network model in combination with an immunomarker expression characteristic matrix; spatial interaction characteristics of a tumor microenvironment are modeled by adopting a graph attention mechanism, finally a treatment response probability, an optimal treatment opportunity and an adverse reaction risk are predicted through a multi-task learning framework, and a clinical decision report containing a prediction response curve, a risk early warning threshold and a treatment time window suggestion is output. According to the method, through multi-modal data fusion and spatial interaction modeling, accurate prediction of intestinal cancer immunotherapy response is realized, and a more comprehensive reference basis is provided for clinical decision making.

The invention discloses a drug target affinity prediction method and system based on a multi-scale protein attention mechanism, and belongs to the crossing field of bioinformatics and artificial intelligence. The method comprises the following steps: firstly, extracting protein sequence features through an ESM2 pre-training model, predicting that a three-dimensional structure is converted into a two-dimensional contact graph, and extracting spatial topological information in combination with a graph convolutional network; a two-dimensional attention mechanism is innovatively designed, structural features are taken as query vectors, sequence features are taken as key value pairs, and cross-modal feature fusion is realized by dynamically associating sequence semantics and spatial proximity relationships through multiple attention. Drug molecules are characterized by adopting MACCS fingerprints, are spliced with protein multi-modal features and then are optimized through a deep network, and finally an affinity value is output through a regression prediction module. According to the technology, the problem of protein heterogeneous data fusion is effectively solved, the generalization ability to unknown targets is remarkably improved, an efficient calculation tool is provided for new drug research and development and drug relocation, and the drug research and development cost can be reduced.

The invention provides a method for establishing a diagnosis and treatment system for digestive system disease multi-modal information. The method comprises the following steps: S1, collecting multi-modal information for labeling and preprocessing; s2, extracting a feature vector and embedding a label into the multi-modal information according to the labeled information; s3, splicing and mapping the feature vector and the tag to a unified dimension to obtain an enhanced feature vector; s4, fusing the enhanced feature vectors to form a multi-modal feature matrix, performing linear mapping and weighted aggregation on the multi-modal feature matrix to obtain global fusion vectors, and collecting to generate a fusion vector sequence; s5, enhancing the time sequence information of the global fusion vector sequence, enhancing the spatial information of the spatial relevance of the specific feature of the part, and performing interactive fusion to obtain a spatio-temporal joint feature; s6, performing classification prediction on the disease stage or the specific pathological type, and outputting a diagnosis result; and S7, performing semantic association on the diagnosis result and the medical knowledge graph, sharing data to an online health intelligent platform, and providing a personalized decision basis for clinicians.

A database structure obtained by means of information retrieval, and a reasoning system, which structure and system specifically relate to a gut microbe knowledge graph system, comprising: a gut microbe knowledge graph consisting of a gut microbe knowledge base, a gut microbe and small-molecule drug therapy association knowledge base, and a clinical medicine database; and a multimodal uncertainty reasoning system, using the gut microbe knowledge graph. The gut microbe knowledge graph system predicts potential diseases, drugs, genes, etc., which are associated with gut microbes.

The invention discloses an abnormal monitoring method and system for immune cell culture. The method comprises the following steps: collecting cell physical state parameters, culture environment parameters and metabolic biochemical indexes in a culture system in real time; performing motility rate threshold judgment and morphological analysis based on the cell physical state parameters to generate a first abnormal signal; performing dynamic trend analysis on the metabolism biochemical indexes to generate a second abnormal signal; performing grade association on the culture environment parameters, the first abnormal signal and the second abnormal signal, and outputting a monitoring abnormal grade; and performing grading response construction according to the monitoring abnormity grade to obtain an abnormity monitoring report. According to the method, the pollution diffusion risk and the functional cell failure misjudgment rate can be reduced.

The invention relates to the technical field of biology, in particular to a verticillium dahliae virulence gene, a verticillium dahliae virulence protein and application. The invention discloses a verticillium dahliae virulence gene VdPHO23like. The verticillium dahliae virulence gene VdPHO23like comprises a polynucleotide sequence for coding an amino acid sequence of SEQ ID NO: 3. According to the invention, expression of the gene is inhibited in a targeted manner through an RNA interference mediated gene silencing technology so as to weaken virulence of pathogenic bacteria, and an efficient, specific and environment-friendly comprehensive prevention and control strategy is provided for verticillium wilt of crops such as cotton.

The invention discloses an overlapped cervical cytoplasm region segmentation method based on deep learning and a conditional diffusion model, and relates to the technical field of artificial intelligence analysis of medical images. According to the method, accurate segmentation of the overlapped cytoplasm region in the cervical cell image is realized through a morphological prior guided conditional diffusion process. The method comprises the following steps: constructing a multi-scale cervical cytoplasm mask pair image; designing a cytoplasm specific data enhancement and preprocessing process; building a multi-branch cervical cell morphology perception condition diffusion network; using a self-adaptive multi-scale combination loss function to optimize training; and a hierarchical classifier is adopted to freely guide sampling for reasoning. According to the method, the frequency domain and space domain features are fused, a cellular morphology and statistics priori knowledge base is established, and a strategy of generating complete cytoplasm by adopting non-overlapped parts is adopted, so that the problem that the traditional method is difficult to segment in complex backgrounds and overlapped regions is successfully solved, and reliable technical support is provided for early screening of cervical cancer.

The invention provides a semi-supervised target detection method for a visible light-infrared multi-mode fusion scene. The method comprises the following steps: constructing a semi-supervised visible light-infrared multi-modal image data set based on an LLVIP data set; on the basis of a YOLOv11 model architecture, constructing a target detection model oriented to multi-modal image feature fusion, and training the target detection model by using a semi-supervised visible light-infrared multi-modal image data set in a deep learning end-to-end mode to obtain a trained target detection model; and inputting a to-be-detected multi-modal image into the trained target detection model, and outputting a target detection result of the to-be-detected multi-modal image by the trained target detection model. The method is based on a semi-supervised learning normal form, so that the precision and robustness of target detection in a multi-modal fusion scene are improved, and the requirements for high efficiency and reliability of target recognition in practical application scenes such as intelligent traffic and intelligent security and protection are met.

The invention provides an UTR (Untranslated Region) element H2202 P1-G as well as a construction method and application thereof, and relates to the technical field of mRNA (messenger ribonucleic acid). According to the present invention, the ribosome load prediction and the secondary structure optimization are performed on the natural 5 'UTR of the HIV TAT 202 gene through the BaidleHelix platform, and the obtained HTAT 202 P1 sequence avoids the inhibitory hairpin structure so as to significantly improve the luciferase expression quantity compared to the natural UTR; an ncRNA sequence without a secondary structure is introduced on the basis of the HTAT 202 P1, translation inhibition of a 5 'cap region is further relieved, and the protein expression quantity of the constructed H2202 P1-G mutant (the DNA sequence of the H2202 P1-G is as shown in SEQ NO 1, and the RNA sequence is as shown in SEQ NO 2) is further improved.

Provided herein are methods, compositions, and kits for determining the spatial location of target nucleic acids, including endogenous and exogenous target nucleic acids, in a biological sample using padlock probes and substrates with spatially barcoded capture probes. Also disclosed herein are methods for determining a presence and / or location of a microbe (e.g., archaea, fungi, bacteria) in a biological sample, e.g., by determining the presence and / or location of a microbial target nucleic acid in the biological sample.

The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.

Artificial intelligence enabled disease profiling is described. An electrocardiogram analysis module is configured to derive disease vectors for a plurality of diseases using electrocardiogram training data from both disease-negative and disease-positive individuals. A standardized input is generated, via a data preprocessor of the electrocardiogram analysis module, from an electrocardiogram recorded from an individual. The standardized input is encoded, by a deep learning autoencoder of the electrocardiogram analysis module, into an embedding, the embedding being a lower-dimensional latent space representation of features extracted from the standardized input. At least one disease risk score for the individual is generated, by a statistical modeling algorithm of the electrocardiogram analysis module, for the plurality of diseases based on the embedding and the disease vectors.

The invention belongs to the technical field of medical data processing and artificial intelligence, and particularly relates to a cardiovascular disease risk prediction system based on multi-modal fusion, which comprises a multi-modal data acquisition and preprocessing module, a cross-modal association graph construction module, a dynamic fusion and prediction module based on a graph neural network and an interpretability analysis module. By constructing a heterogeneous graph fusing prior knowledge and data driving and utilizing a graph attention network to perform multi-level dynamic feature fusion, deep integration and interaction of multi-modal data such as genomes, iconography, clinical and intestinal flora metabolism are realized, so that the accuracy and interpretability of cardiovascular disease risk prediction are improved.

The invention relates to the technical field of artificial intelligence, in particular to a pest and disease identification method and system based on image processing and a medium, and the method comprises the steps: obtaining an original image of a plant leaf, and carrying out the preprocessing of the original image, and obtaining a preprocessed image; performing scab region segmentation on the preprocessed image by using an improved U-Net model to obtain a contour and a position of a scab; carrying out feature extraction on the segmented scab region, extracting deep semantic features through a pre-trained ResNet-50 network, and carrying out splicing fusion on the deep semantic features and color features and shape features of the scab to form a comprehensive feature vector; inputting the comprehensive feature vector into an integrated classifier based on XGBoost to carry out disease and insect pest type identification, and outputting disease and insect pest types and corresponding probabilities; the accuracy of pest and disease prediction can be improved.

The invention provides a brain tumor survival prediction method and system based on a multi-modal medical knowledge graph, and belongs to the technical field of brain tumor survival prediction. The multi-modal medical knowledge graph based on third-party knowledge base fusion is constructed; performing feature extraction on the brain tumor multi-modal data; searching an entity corresponding to the brain tumor related data in the multi-modal medical knowledge graph, and converting the entity into feature representation by using an entity representation learning method; the learned feature representation related to the brain tumor type complements the missing data mode, and finally the complemented features are input into a pre-trained survival prediction model to achieve brain tumor survival prediction. According to the multi-modal medical knowledge graph, comprehensive medical knowledge support meeting clinical requirements is provided; the multi-modal mapping knowledge domain is used for missing modal completion of brain tumor survival prediction, and a completion feature is generated by querying an associated entity through the mapping knowledge domain, so that the problem of weak modal missing processing capability in the prior art is solved.

The invention relates to the technical field of brain-computer interfaces, and provides a brain-computer interface instruction issuing method, device and equipment based on regulation enhancement simulation, and the method comprises the steps that an electroencephalogram decoding model comprises an encoder, a feature enhancer and a task classifier, the encoder encodes a real-time electroencephalogram signal to obtain compression representation before nerve regulation, and the feature enhancer is used for classifying the compression representation before nerve regulation; the feature enhancer performs feature enhancement on the compression representation to obtain enhanced representation, and the task classifier classifies the enhanced representation to obtain an electroencephalogram decoding result. According to the method, a feature enhancer is obtained by combining training of a state discriminator based on a sample electroencephalogram signal collected before nerve regulation and a real state label after nerve regulation, and the feature enhancer is driven to learn a feature migration relation between a compression feature before nerve regulation and a feature after nerve regulation; the feature characterization capability of an electroencephalogram decoding model on electroencephalogram signals is remarkably improved, so that the decoding robustness on weak stimulation signals is enhanced on the premise of not depending on high-intensity external stimulation.

A deep neural network(s) (DNN) may be used to detect objects from sensor data of a three dimensional (3D) environment. For example, a multi-view perception DNN may include multiple constituent DNNs or stages chained together that sequentially process different views of the 3D environment. An example DNN may include a first stage that performs class segmentation in a first view (e.g., perspective view) and a second stage that performs class segmentation and / or regresses instance geometry in a second view (e.g., top-down). The DNN outputs may be processed to generate 2D and / or 3D bounding boxes and class labels for detected objects in the 3D environment. As such, the techniques described herein may be used to detect and classify animate objects and / or parts of an environment, and these detections and classifications may be provided to an autonomous vehicle drive stack to enable safe planning and control of the autonomous vehicle.

The invention provides a multi-modal characterization molecular property prediction method based on hierarchical bidirectional cross attention, and relates to the technical field of machine learning assisted organic chemistry, and the method comprises the following steps: S10, generating same-molecule multiple sequences for data enhancement; s20, coding the sequence features through a pre-trained molecular language model MolBERT; s30, performing multi-modal feature fusion through a layered bidirectional cross attention mechanism; s40, establishing a prediction head; s50, in the reasoning stage, only the feature extraction and fusion steps are executed, and a molecular property prediction result is output through the trained prediction head. According to the method, the molecular sequence, the topological graph structure and the fingerprint features are effectively integrated, so that the prediction precision of the model on a plurality of MoleculeNet (molecular network benchmark) public data sets is superior to that of an existing method.

The invention discloses a body feeling evaluation method based on electroencephalogram-electromyographic signal fusion and dynamic interaction modeling. The body feeling evaluation method comprises the steps that EEG signals and EMG signals in the lower limb movement process of a subject are synchronously collected; carrying out band-pass filtering, artifact removal and wavelet transform processing on the acquired signals, extracting multi-channel time-frequency features, and forming a preprocessing feature matrix; fusing the time-frequency features of the EEG signal and the EMG signal, constructing a multi-modal feature set, and compressing feature dimensions by adopting a sparse coding method; inputting the compressed feature sequence into a neural network model combining a long short-term memory network and an attention mechanism, and carrying out dynamic interaction modeling; and an interaction index sequence is generated based on model output, and an interaction matrix is constructed through a sliding window and Gaussian kernel smoothing processing, so that visualization of brain-muscle interaction strength and dynamic quantification of a proprioceptive function are realized. The invention further provides a system for implementing the method. The method is high in objectivity, high in feature extraction precision and excellent in dynamic modeling capability.

The invention belongs to the field of molecular biological identification, and particularly relates to a specific SNP (Single Nucleotide Polymorphism) site combination for identifying a Wuzhishan pig variety and application. The specific SNP site combination for identifying the Wuzhishan pig variety comprises 77 SNP sites, and the physical positions of the SNP sites are determined by sequence comparison based on a pig reference genome Sscrofa11.1. The specific SNP site combination is screened based on a method of combining whole genome association analysis with selection signal analysis so as to ensure the accuracy of site selection. The selected specific SNP site combination can rapidly realize accurate identification of the Wuzhishan pig variety on the gene level, and has significant application value in the aspects of genetic resource accurate protection and variety utilization of Hainan Wuzhishan pigs.

The invention discloses an adverse drug reaction event recognition method and system based on multivariate knowledge mixed retrieval enhancement, and the method comprises the steps: extracting drug entities from a to-be-recognized clinical disease course record, segmenting the disease course record, and obtaining a drug entity set and a sentence set; for each extracted drug entity, retrieving drug concept knowledge having a hyponymy relationship with the drug entity and drug adverse reaction knowledge having an adverse reaction relationship with the drug entity in a pre-constructed multivariate knowledge base; for each sentence obtained through segmentation, searching suspected adverse drug reaction events meeting a first similarity requirement and drug field text knowledge meeting a second similarity requirement in a pre-constructed multivariate knowledge base; and finally, calling a large language model, taking all the retrieved knowledge as reference knowledge, and identifying the adverse drug reaction event from the to-be-identified clinical disease course record. According to the invention, the accuracy and reliability of adverse drug reaction event identification can be improved.

The invention provides an UTR element NHP1 as well as a construction method and application thereof, and relates to the technical field of mRNA. A 5 'UTR with a good expression effect is designed by integrating dominant sequences of a human high-expression gene and a pathogen natural UTR, a chimeric structure NHP1 with high ribosome load is predicted through a calculation model, a DNA sequence of the NHP1 is as shown in SEQ NO 1, and an RNA sequence of the NHP1 is as shown in SEQ NO 2; an EGFP report system is adopted on the DNA level to rapidly screen UTR; the translation efficiency is quantitatively evaluated on the RNA level through luciferase mRNA (N1-methyl pseudouridine modification); and the particle size is controlled by a microfluidic technology, so that the optimized UTR-mRNA is efficiently expressed after being delivered.

The invention belongs to the technical field of radar signal processing, and discloses a sea surface small target detection method based on optimized characteristic mode decomposition, which comprises the following steps: S1, acquiring to-be-detected signal data; s2, decomposing an original signal into a plurality of modal components by using FMD, and selecting an envelope spectrum entropy as a fitness function; s3, performing global optimization on the fitness function in the FMD by using an SOS algorithm; s4, introducing a PSO algorithm to carry out local optimization on key parameters of the FMD; s5, components with low envelope spectrum entropy values and correlation coefficients larger than a threshold value are reserved; s6, extracting an envelope spectrum entropy and frequency band energy ratio feature from the screened modal components, introducing a Gini coefficient as a weighting factor, and constructing a GSEBE joint feature; and S7, inputting the entropy value of the envelope spectrum into a DELM classifier with a controllable false alarm, and realizing target detection based on comparison between a predicted value and a judgment threshold. According to the invention, the capability of distinguishing sea clutters and target echoes is enhanced, and more accurate classification detection is realized.

The invention discloses a colorectal cancer drug relocation method based on multi-omics integration. The system comprises a multi-omics data acquisition and preprocessing module, a tumor microenvironment analysis module, a specific disease network construction module, a multi-dimensional drug relocation module and a result evaluation module. And the tumor microenvironment analysis module comprises cell heterogeneity identification, cell map construction, cell annotation and tumor cell subset annotation. The specific disease network construction module comprises tumor feature expression program extraction, expression program screening, meta-program construction, clinical related meta-program recognition and specific disease protein interaction network construction. And the multi-dimensional drug relocation module comprises a module for identifying diseases by using a random walk algorithm, carrying out drug screening based on disturbance data, carrying out drug screening based on network proximity and carrying out comprehensive drug relocation. From the perspective of single cell data, element programs related to colorectal cancer survival are excavated, corresponding modules are designed, and the efficiency and precision of colorectal cancer targeted drug screening are improved.

The invention discloses a bird identification method based on sound-image multi-modal fusion. The bird identification method comprises the following steps: S1, carrying out standardized frame-level preprocessing on bird audio signals; s2, acoustic features are extracted and enhanced, and an acoustic high-level feature vector which highlights birdsong discrimination information and suppresses environmental noise is obtained; s3, visual image standardization preprocessing; s4, performing visual feature extraction and multi-scale fusion to obtain a visual high-level feature vector which enhances correspondence to the bird key form area and inhibits background interference; s5, performing dynamic weighted fusion on the decision-making layer to obtain a bird existence probability; and S6, comparing the bird existence probability with a preset threshold value of the corresponding bird, and judging whether the bird exists or not and the type of the existing bird. Through cross-modal feature enhancement and adaptive fusion, the precision, robustness and real-time performance of bird recognition in a complex orchard environment are significantly improved, and a core technical support is provided for green intelligent bird repelling.

The invention discloses a multi-mode emotion continuous recognition method for medical treatment, belongs to the technical field of artificial intelligence and medical treatment information, and mainly aims to simulate the dynamic change process of emotion by establishing a Neural ODEs framework and overcome the static property and discreteness of emotion modeling in a traditional method. Through a causal inference technology, emotional features are separated from individual-independent physiological differences, and the generalization ability across individuals is improved. A self-supervised learning method is utilized, the synergistic effect between the EEG and the eye movement signal is improved through cross-modal contrast learning, and the emotion recognition precision is enhanced. The calculation complexity is reduced through a dynamic sparse attention mechanism, and meanwhile, focusing is performed on a key time slice in emotion recognition. Through multi-task joint learning, the model learns multiple tasks such as emotion intensity regression and tested identity recognition during emotion classification, and the personalized emotion recognition capability is improved.