60818 results about "Bioinformatics" patented technology

The technology described herein relates to systems, methods, and computer storage media, among other things, for providing search query intent specificity. Embodiments may include identifying a search query performed using a search engine and generating a query vector for the search query by aggregating search result embeddings (e.g., item listing vectors) of search results from the search query. Further, in some embodiments, similarities (e.g., cosine similarities) between the query vector and the item listing vectors can be determined. As such, an intent specificity of the search query can be determined. Further, in some embodiments, the intent specificity can be used to train an intent specificity machine learning model for generating intent specificity scores for other search queries. Based on the intent specificity scores determined using the one or more trained intent specificity machine learning models, determinations can be made with respect to precision and recall, etc.

Provided herein are methods of identifying a methylation status of an analyte in a biological sample. Also provided herein are methods that combine identifying the methylation status with spatial technology to identify the location of a methylation status in a biological sample.

Provided herein are methods, compositions, and systems for improved in situ detection of analytes and spatial analysis using, e.g., a sequencing readout.

Provided herein are methods of identifying abundance and location of an RNA in a biological sample using an adaptor sequence and a primer. Also disclosed herein are kits, compositions, and systems that are used to perform the methods.

The present disclosure features methods, compositions, and kits for determining the location of a target nucleic acid and / or detecting the presence or absence of a genetic variant in a biological sample using an array with capture probes comprising a spatial barcode and one probe which has complementarity to the capture probe, binds to target and is extended. A second probe which binds to the extended first probe and is extended and bound to capture probe. Location and genetic variant are determined by sequencing of the extended probe.

The present disclosure features methods, compositions, and kits for the spatial detection of genetic variants in a biological sample by using branched ligation.

The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.

Provided herein are methods, compositions, and kits to spatially detect genetic variants in a target nucleic acid via templated-ligation and reversible terminator nucleotides.

The method can include: recording a neural state, determining a latent array, and determining an output based on the latent array. In variants, the method can function to enable a subject to use neural signals to navigate within a high-dimensional concept space to control an output (e.g., image generation, motor control, communication, etc.).

The present disclosure relates to materials and methods for spatially analyzing nucleic acids that have been fragmented with a transposase enzyme, alone or in combination with other types of analytes.

Provided herein are methods, compositions, and kits for the spatial analysis of target nucleic acids, or complements thereof, by their 5′ end.

The present disclosure relates to materials and methods for spatially analyzing nucleic acids that have been fragmented with a transposase enzyme, alone or in combination with other types of analytes.

Provided herein are methods of identifying abundance and location of an RNA in a biological sample using an adaptor sequence and a primer. Also disclosed herein are kits. compositions, and systems that are used to perform the methods.

Disclosed herein are methods of amplifying an analyte in a biological sample using a bridging oligonucleotide that hybridizes to a captured analyte. The methods disclosed herein include steps of (a) contacting a biological sample with a substrate having capture probes comprising a capture domain and a spatial barcode; (b) hybridizing the analyte to the capture domain; and (c) contacting the analyte to a bridging oligonucleotide comprising (i) a capture-probe-binding sequence, and (ii) an analyte-binding sequence; (d) extending the bridging oligonucleotide; and (e) determining (i) all or a part of the sequence of the analyte, or a complement thereof, and (ii) the spatial barcode, or a complement thereof, and using the determined sequence of (i) and (ii) to determine the location of the analyte in the biological sample.

Provided herein are methods, kits, and compositions for determining the spatial location of non-coding RNA, such as siRNA and / or miRNA in a biological sample.

The present disclosure relates to materials and methods for spatially analyzing nucleic acids that have been fragmented with a transposase enzyme, alone or in combination with other types of analytes.

The present disclosure provides methods and compositions for detecting and spatially locating analyte interactions and gene expression in a biological sample. For example, provided herein are methods of determining a location of at least one analyte in a biological sample using analyte-binding moieties, proximity ligation, and an array including capture probes.

The present disclosure provides systems and methods for processing or analyzing a sample. Methods may include generating supports (e.g., beads) comprising barcode molecules coupled thereto. A support comprising barcode molecules may be useful for analyzing or processing one or more analytes such as nucleic acid molecules, proteins, and / or perturbation agents.

The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.

The invention discloses a pediatric abnormal breathing detection method and system based on a physiological signal, and relates to the technical field of medical information processing, and the method comprises the steps: collecting a multi-modal physiological signal, employing wavelet decomposition and a soft threshold function to eliminate noise, and outputting a preprocessing signal group in combination with morphological opening and closing operation; calculating a time domain feature, a frequency domain feature and a nonlinear feature of the preprocessed signal group, organizing the features into a three-layer pyramid structure, analyzing time sequence similarity by adopting an attention mechanism, and outputting a fusion feature sequence; and carrying out preliminary anomaly detection on the fused feature sequence, carrying out classification based on an age grouping threshold, extracting context feature vectors of windows before and after an abnormal point, and carrying out secondary discrimination to realize accurate identification and graded early warning of the respiratory anomaly. The respiration monitoring system with high reliability, high adaptability and intelligent learning ability is constructed, and comprehensive technical support is provided for early recognition, real-time early warning and intelligent diagnosis of children respiratory system diseases.

The present disclosure relates to materials and methods for spatially analyzing nucleic acids that have been fragmented with a transposase enzyme, alone or in combination with other types of analytes.

Methods and compositions to minimize barcode exchange during the preparation of barcoded next-generation sequencing libraries prepared from a single cell. The methods utilize oligonucleotides containing a 3′-terminated blocking group or sequences that prevent amplification or extension.

Provided herein are methods and compositions disclosing an approach that combines single nuclear RNA-seq (snRNA-seq) with spatial transcriptomics. The methods include processing and analyzing multiple analytes in a biological sample, and utilizes multiple substrates, one having a spatial array having a plurality of capture probes, each having a capture domain and a spatial barcode. The methods can be used to detect location of both nucleic acid and protein analytes. The methods also include isolating cells and / or nuclei from a sample and determining analyte expression in the isolated cells and / or nuclei.

Provided herein are methods of determining a location of a biological analyte in a biological sample.

The present disclosure relates to materials and methods for spatially analyzing nucleic acids that have been fragmented with a transposase enzyme, alone or in combination with other types of analytes.

Provided herein are methods for preparing biological samples for spatial proteomic analysis, methods of determining a location of a protein analyte in a biological sample, and methods of determining a location of a protein analyte and a nucleic acid analyte in a biological sample.

The present disclosure features methods, compositions, and kits for spatially barcoded, reusable master oligonucleotide probes.