1014results about "Sequential/parallel process reactions" patented technology

Disclosed are extended and / or branched oligonucleotide capture probe assemblies for use in spatial transcriptomics systems, and methods for making the capture probe assemblies.

The invention is directed to methods and systems for producing a surface coated with a plurality of spatial barcodes. In one aspect, beads carrying barcode oligonucleotides are disposed on a surface after which hydrogel chambers are synthesized around each bead and barcode oligonucleotides are released to be captured on the surface in the interiors of the chambers. Captured oligonucleotide barcodes are amplified and optionally sequenced to form a coating of spatial barcodes.

Nucleic acid hybridization buffer formulations and uses thereof are described that yield improvements in hybridization specificity, rate, and efficiency. The buffer formulation composition includes a target nucleic acid; at least one organic solvent having a dielectric constant in the range of no greater than 115; and a pH buffer system, wherein the target nucleic acid is attached to the surface via hybridization to a surface bound nucleic acid tethered to the surface, and wherein the hybridization of the target nucleic acid and surface bound nucleic acid has a high stringency and annealing rate.

The invention provides a wind-solar hydrogen production and ammonia synthesis system and a configuration method thereof, electronic equipment and a storage medium. The wind and light hydrogen production and ammonia synthesis system comprises a wind and light hydrogen production subsystem, an ammonia synthesis subsystem and a coordination controller which are relatively independent, the ammonia synthesis subsystem comprises a hydrogen storage tank, the hydrogen storage tank is connected with the wind and light hydrogen production subsystem, and the configuration method of the wind and light hydrogen production and ammonia synthesis system can be executed by the coordination controller. The configuration method of the wind-solar hydrogen production ammonia synthesis system comprises the following steps: firstly, determining capacity configuration parameters of a wind-solar hydrogen production subsystem by taking the minimum leveling hydrogen production cost as a target; and determining the capacity configuration parameters of the ammonia synthesis subsystem based on the capacity configuration parameters of the wind-solar hydrogen production subsystem by taking the maximization of the wind-solar absorption rate, the minimization of the total cost of the system and the minimization of the operation cost of the system as targets. The wind and light hydrogen production subsystem and the ammonia synthesis subsystem are subjected to two-stage decoupling type system configuration, and stable operation of the ammonia synthesis process is ensured while efficient utilization of green electricity is achieved.

The invention provides a preparation device and process of electronic-grade silane. According to the preparation device, a first disproportionation reaction is carried out on the basis of the first reactor, dichlorosilane is rectified and separated to serve as a raw material for a second disproportionation reaction, silane can be obtained on the basis of cheap trichlorosilane and a catalytic reagent, and electronic-grade silane can be obtained on the basis of traditional purification after rectification; based on heat exchange between tower top materials of the rectifying tower I and a tower kettle reboiler of the rectifying tower II, differential pressure thermal coupling integration is realized, condensing equipment and operation steps can be omitted, energy consumption is reduced, and the defects of high preparation cost and high energy consumption of a silane preparation device in the prior art are overcome.

The invention discloses a continuous synthesis and purification method of hexafluoropropylene oxide, which comprises the following steps: introducing hexafluoropropylene, oxygen and a fluorine-containing solvent into a supergravity reactor to carry out oxidation reaction at the reaction temperature of 100-200 DEG C under the reaction pressure of 1.0-2.5 Mpa for 10-120 seconds, and after the reaction is finished, overflowing and leading out reaction liquid to obtain the hexafluoropropylene oxide. And purifying to obtain the hexafluoropropylene oxide. According to the method, the problems of mass transfer and heat transfer can be effectively solved by using the supergravity reactor, the reaction selectivity is remarkably improved, meanwhile, the productivity is effectively improved, and the supergravity reactor is combined with extractive distillation treatment, so that the conversion rate of the hexafluoropropylene reaches 79% or above, the selectivity of the hexafluoropropylene oxide is 85% or above, and the purity of the hexafluoropropylene oxide after extractive distillation reaches 99%. The invention further discloses a device used in the continuous synthesis and purification method, and cyclic utilization of hexafluoropropylene and the fluorine-containing solvent can be achieved.

Polymers synthesized by solid-phase synthesis are selectively released from a solid support by reversing the bias of spatially addressable electrodes. Change in the current and voltage direction at one or more of the spatially addressable electrodes changes the ionic environment which triggers cleavage of linkers that leads to release of the attached polymers. The spatially addressable electrodes may be implemented as CMOS inverters embedded in an integrated circuit (IC). The IC may contain an array of many thousands of spatially addressable electrodes. Control circuity may independently reverse the bias on any of the individual electrodes in the array. This provides fine-grained control of which polymers are released from the solid support. Examples of polymers that may be synthesized on this type of array include oligonucleotides and peptides.

The invention provides a methanol production system comprising a reverse water gas reaction module having a first in-situ separation reactor configured to perform reverse water gas conversion of carbon dioxide and hydrogen, generate and in-situ separate carbon monoxide and water, a methanol synthesis module connected to the reverse water gas reaction module, and the second in-situ separation module is provided with a second in-situ separation reactor, and the second in-situ separation reactor is configured to generate and separate methanol in situ by utilizing the carbon monoxide generated by the reverse water gas reaction module to react with hydrogen. According to the scheme provided by the invention, a two-step methanol preparation process and the in-situ separation type reactor are organically coupled, so that the conversion of carbon dioxide can be completed under a relatively mild working condition; the problems of low one-way conversion rate, high recycling compression work and the like in methanol preparation through carbon dioxide hydrogenation are solved; the energy consumption for separating water and methanol is saved; the methanol production cost is further reduced, and the reliability and economy of the system are improved.

A preparation equipment and a process for a nylon salt solution. The preparation equipment includes a suspension preparation device, a first salt formation reactor and a second salt formation reactor. The suspension preparation device includes a feeding unit, a continuous feeding unit, a high-shear pump and a mixing reactor, the high-shear pump and the mixing reactor are cyclically connected with and in communication with each other through two connecting pipelines; the second salt formation reactor includes a second diamine feed pipe, a third diamine feed pipe, a circulation pipeline of the second salt formation reactor and an online near-infrared monitoring equipment located on the circulation pipeline of the second salt formation reactor.

A method comprising receiving a transaction request to print a genetic sequence, the transaction request including an account and a key, a state of the account managed by a blockchain where a plurality of copies of the blockchain include a state of the account; retrieving a stored key associated with the account; authenticating the transaction request based on the stored key and the transaction request key; determining a state of the account in a first copy of the blockchain is the same as in at least a second copy of the blockchain; validating the transaction request based on the determining a state of the account in a first copy of the plurality of copies of the blockchain is the same as in at least a second copy of the plurality of copies of the blockchain; and authorizing the transaction request based on the authentication and validation of the transaction request.

The invention relates to a nylon 6 polymerization production method capable of fully recycling extractable oligomers, and relates to the technical field of nylon 6 polymerization production processes, the nylon 6 polymerization production method comprises a nylon 6 polymerization section, a nylon 6 slice extraction section, a nylon 6 wet slice drying section, an extract evaporation and concentration section and an extractable oligomer cracking and recycling section; in an extractable oligomer cracking and recycling section, by adjusting process conditions such as temperature, pressure and water content of an oligomer extraction concentrated solution, deep cracking of extractable substances is realized, and all cracking products can be recycled into a polymerization section for recycling, so that rapid accumulation of cyclic dimers in polymers is inhibited, no residues are generated in the polymerization process, and the production cost is reduced. Accumulation of cyclic dimers in a system can be effectively slowed down, the service life of a polymerization reactor is prolonged, and the quality of polymer products is improved.

High surface area coatings are applied to solid substrates to increase the surface area available for solid-phase synthesis of polymers. The high surface area coatings use three-dimensional space to provide more area for functional groups to bind polymers than an untreated solid substrate. The polymers may be oligonucleotides, polypeptides, or another type of polymer. The solid substrate is a rigid supportive layer made from a material such as glass, a silicon material, a metal material, and plastic. The coating may be thin films, hydrogels, microparticles. The coating may be made from a metal oxide, a high-κ dielectric, a low-κ dielectric, an etched metal, a carbon material, or an organic polymer. The functional groups may be hydroxyl groups, amine groups, thiolate groups, alkenes, n-alkenes, alkalines, N-Hydroxysuccinimide (NHS)-activated esters, polyaniline, aminosilane groups, silanized oxides, oligothiophenes, and diazonium compounds. Techniques for applying coatings to solid substrates and attaching functional groups are also disclosed.

An example flow cell includes a first substrate; a second substrate attached to the first substrate; a flow channel defined between the first substrate and the second substrate; a first primer set attached to the first substrate, the first primer set including an un-cleavable first primer and a cleavable second primer; a second primer set attached to the second substrate, the second primer set including a cleavable first primer and an un-cleavable second primer; and a removable blocking mechanism passivating i) the first primer set or the second primer set or ii) the cleavable first primer and the cleavable second primer.

The invention relates to a preparation method and a preparation device of isocyanate, and the preparation method comprises the following steps: carrying out cold reaction on a phosgene solution and an amine solution in a cold reactor to obtain an intermediate mixed solution; separating the intermediate mixed solution by adopting a cyclone, obtaining a carbamoyl chloride mixed solution from an underflow port of the cyclone, and obtaining an amine hydrochloride mixed solution from an overflow port of the cyclone; the carbamyl chloride mixed solution enters a first thermal reactor for thermal reaction to obtain a first reaction product, and the amine hydrochloride mixed solution enters a second thermal reactor for thermal reaction to obtain a second reaction product; mixing the first reaction product and the second reaction product in a staying reactor to obtain an isocyanate reaction solution; and carrying out post-treatment on the isocyanate reaction liquid to obtain the isocyanate. The method can reduce the generation amount of urea by-products, further inhibits the generation of other subsequent by-products, reduces the chromaticity of the product, and improves the quality of isocyanate.

A Solid Phase Peptide Synthesis (SPPS) device and method of using the same for manufacturing peptides is taught herein. The system comprises at least two reactors, each reactor including a quantity of SPPS resin. The reactors are positioned in series. A de-protecting agent is added to the first reactor and then transferred to the second and third reactors, in series, thereby operating to de-protect the protected N-group. Wash solvent is added to the first reactor and then transferred to the second and this operation repeated several times. Likewise, an amino acid activated ester solution is added, in series, to the first, second and third reactors, thereby operating to couple the amino acid to the de-protected N-group. Wash solvent is added to the first reactor and then transferred to the second and this operation repeated several times prior to the next cycle. The use of the reactors in series reduces the overall solvent required. Online LCMS is also used to monitor progress and identity of reactions happening within the solid phase resin particles.

The invention relates to the technical field of micro-channel equipment extraction, in particular to a micro-channel extraction reactor with a grid plug-in and application of the micro-channel extraction reactor. The microchannel extraction reactor includes: a microchannel panel; an extraction unit; comprising a containing groove, a linear micro-channel, a front chamber and a rear chamber, the stainless steel filter screen set comprises at least one slightly-curved stainless steel filter screen arranged in the containing groove, the protruding end of the slightly-curved stainless steel filter screen faces the containing groove, and the slightly-curved stainless steel filter screen is arranged between a micro-channel panel and the containing groove; each adjusting unit comprises a sliding supporting piece and an adjusting groove for containing the sliding supporting piece, the sliding supporting pieces stretch out and draw back through the adjusting grooves, and one end of each sliding supporting piece is fixedly connected with the corresponding slightly-bent stainless steel filter screen. The micro-channel extraction reactor can promote sufficient mass transfer of phosphoric acid on an extraction phase interface so as to improve the separation effect of metal element impurities in a phosphoric acid raw material under different flow velocity conditions.

The invention provides a method for the exploration of chemical space through a multigenerational series of synthetic stages. In a first stage, a series of reactions is performed. The products of the first series are analysed, and a first product from the series of reactions is selected. The first product is used as a chemical input for each of the reactions in a second series of reactions. The products of the second series are analysed. The first and second stages are performed autonomously. The selection of a product in the first stage comprises the comparison of the products from the first series of reactions against a fitness function, where the selected product has a superior fitness compared with one or more other products in the first series. Each reaction in the first series differs in one or more chemical and / or physical inputs. Each reaction in the second series differs in one or more chemical and / or physical inputs. The invention also provides an automated exploration apparatus for performing the method, and the apparatus comprises a controller, and a chemical synthesiser and an analytical unit which are operable by the controller, as described herein.

The invention provides an inlet valve array, a cleaning method and a biosynthesis system based on the inlet valve array. A first connecting flow channel and a second connecting flow channel of the inlet valve array are connected with a cleaning main pipeline through pipelines; outlets of the first connecting flow channels of the two inlet valve arrays are connected with a first liquid inlet main pipeline through pipelines. A first connecting flow channel of one inlet valve array is connected with a second liquid inlet main pipeline through a pipeline, the first liquid inlet main pipeline and the second liquid inlet main pipeline are respectively connected with a column front input pipeline, and the column front input pipeline is connected with the input ends of a plurality of composite columns through a plurality of valves; an output end pipeline of each composite column is connected with an inlet of a post output pipeline through a corresponding valve; an outlet of the post-column output pipeline is connected with a discharge pipeline and a second liquid inlet main pipeline through valves; the device has the advantages that cross contamination generated when different materials are conveyed can be avoided, the synthesis purity is improved, and waste of the materials is reduced, so that the cost is reduced; and the pump can be switched without stopping.

A method comprising receiving a transaction request to print a genetic sequence, the transaction request including an account and a key, a state of the account managed by a blockchain where a plurality of copies of the blockchain include a state of the account; retrieving a stored key associated with the account; authenticating the transaction request based on the stored key and the transaction request key; determining a state of the account in a first copy of the blockchain is the same as in at least a second copy of the blockchain; validating the transaction request based on the determining a state of the account in a first copy of the plurality of copies of the blockchain is the same as in at least a second copy of the plurality of copies of the blockchain; and authorizing the transaction request based on the authentication and validation of the transaction request.

Selectively controllable cleavable linkers include electrochemically-cleavable linkers, photolabile linkers, thermolabile linkers, chemically-labile linkers, and enzymatically-cleavable linkers. Selective cleavage of individual linkers may be controlled by changing local conditions. Local conditions may be changed by activating electrodes in proximity to the linkers, exposing the linkers to light, heating the linkers, or applying chemicals. Selective cleaving of enzymatically-cleavable linkers may be controlled by designing the sequences of different sets of the individual linkers to respond to different enzymes. Cleavable linkers may be used to attach polymers to a solid substrate. Selective cleavage of the linkers enables release of specific polymers from the solid substrate. Cleavable linkers may also be used to attach protecting groups to the ends of growing polymers. The protecting groups may be selectively removed by cleavage of the linkers to enable growth of specific polymers.

Compositions and methods are provided for forming single-analyte arrays with enhanced characteristics for inhibiting orthogonal binding of molecules to the array. Arrays are modified to contain covalently incorporated passivating moieties at array addresses where orthogonal binding may occur. The compositions and methods may facilitate detection of an increased quantity of array features at single-analyte resolution.

The invention is directed to methods for massively parallel template-free enzymatic synthesis of a plurality of different polynucleotides of predetermined sequences. In one aspect, methods of the invention employ large scale arrays of reaction sites each associated with at least one working electrode for controlling deprotection and deblocking steps at predetermined user selected sites. In another aspect, the invention provides template-free enzymatic synthesis with proofreading, wherein completed polynucleotides at predetermined reaction sites are sequenced using a sequencing by synthesis technique, particularly employing electrochemically labile blocking groups.

Provided are systems and methods for analyte detection and analysis. A system can comprise an open substrate. The open substrate may be configured to rotate or otherwise move. The open substrate can comprise an array of individually addressable locations, with analytes immobilized thereto. The substrate may be spatially indexed to identify nucleic acid molecules from one or more sources, and / or sequences thereof, with the respective one or more sources. A solution comprising a plurality of probes may be directed across the array to couple at least one of the plurality of probes with at least one of the analytes to form a bound probe. A detector can be configured to detect a signal from the bound probe via scanning of the substrate while minimizing temperature fluctuations of the substrate or optical aberrations caused by bubbles.

The invention is directed to methods for massively parallel template-free enzymatic synthesis of a plurality of different polynucleotides of predetermined sequences. In one aspect, methods of the invention employ large scale arrays of reaction sites each associated with at least one working electrode for controlling deprotection and deblocking steps at predetermined user selected sites. In another aspect, the invention provides template-free enzymatic synthesis with proofreading, wherein completed polynucleotides at predetermined reaction sites are sequenced using a sequencing by synthesis technique, particularly employing electrochemically labile blocking groups.

Embodiments of the present disclosure relate to patterned substrates with functionalized surface such as flow cells, as well as methods of fabricating the patterned substrate. In particular, patterned substrates of the present disclosure may be prepared using two or more imprint resin layers, one of which acts as a photomask for the photoresist during substrate patterning, without the need of any metallic photomask. Embodiments of the patterned substrate may be used for simultaneous paired-end sequencing methods.

The invention provides a production device and process for recovering electronic-grade phosphoric acid from waste etching liquid. The device comprises a raw material storage tank, a multi-stage filtration system, a phosphoric acid evaporation kettle, a purification system, a mixed acid storage tank and a neutralization reaction and crystallization system. The waste etching liquid enters an evaporation kettle after being subjected to multi-stage filtration to remove suspended solid particles, and phosphoric acid is separated from volatile nitric acid and acetic acid; and condensing the volatile acid into mixed acid for storage, and removing metal ions from the semi-finished phosphoric acid through a purification system to obtain the electronic-grade phosphoric acid. The mixed acid reacts with alkali to generate a mixed solution of sodium acetate and nitric acid, sodium acetate crystals are separated out through evaporation and concentration, a solid product is obtained after separation, nitric acid is obtained by condensing gas, and suspended particles and metal impurities are subjected to incineration treatment. Through the steps of filtering, negative-pressure evaporation, neutralization and crystallization and the like, the process realizes efficient recovery of electronic-grade phosphoric acid and byproducts of sodium acetate and nitric acid, and has the characteristics of high resource recovery rate and low pollution emission.

The electronic-grade silane gas continuous production preparation system comprises a cold hydrogenation treatment device, an inlet of the cold hydrogenation treatment device is connected with a raw material supply system, and an outlet of the cold hydrogenation treatment device is connected with a cold hydrogenation rectifying tower through a pipeline. The cold hydrogenation rectifying tower is sequentially connected with the boron and phosphorus removal adsorption column, the first-stage disproportionator, the first tower, the second tower, the second-stage disproportionator, the third tower and the fourth tower through pipelines, and electronic-grade silane in the fourth tower is used for being conveyed to a silane tank area; the first tower is used for removing silicon tetrachloride produced after the disproportionation reaction of the first-stage disproportionator; the second tower is used for separating the extracted chlorosilane material; the third tower is used for separating silane from chlorosilane; and the fourth tower is used for discharging light impurities and heavy impurities and conveying gas-phase electronic-grade silane into a silane tank area. The device and the method realize continuous, efficient and high-purity production of the electronic-grade silane gas, and have the advantages of low energy consumption, low cost, thorough impurity removal and the like.

The invention relates to a device for examining electrochemical processes in a plurality of electrochemical reactor units. The device has a reaction device with a plurality of electrochemical reactor units, a fluid supply unit, and an electroanalytical measuring device, wherein each of the electrochemical reactor units of the reaction device is designed to carry out a respective electrochemical reaction, and wherein each of the electrochemical reactor units has a plurality of electrodes which are designed to be connected to the electroanalytical measuring device. The fluid supply unit is designed to supply each of the plurality of electrochemical reactor units with fluids for carrying out the electrochemical reaction, and the electroanalytical measuring device is designed to examine electrochemical processes occurring in the plurality of electrochemical reactor units when the respective electrochemical reaction is carried out. At least one of the electrochemical reactor units has a plurality of components, wherein the reactor unit is composed of the plurality of components, and wherein an impedance of each of the plurality of components is known, and the device is designed to carry out a characterisation of the electrochemical processes in the plurality of reactor units, wherein, during the characterisation, measured values of the respective reactor unit are compared with measured values of the reactor unit composed of the components with known impedances.

A system and method for automated synthesis of a macromolecule from a nucleic acid template is provided, which includes a flow cell with a sipper, a mount with a thermal block, a pump, a selectable valve, a holder, an XYZ gantry, and a controller. The flow cell can be held in a fixed position while the gantry can move the holder to sippers of the flow cell. In this configuration, the sipper length can be reduced or minimized to reduce loss of precious reagents and the synthesized macromolecule.