206results about "Sequential/parallel process reactions" patented technology

Provided are systems and methods for analyte detection and analysis. A system can comprise an open substrate. The open substrate may be configured to rotate or otherwise move. The open substrate can comprise an array of individually addressable locations, with analytes immobilized thereto. The substrate may be spatially indexed to identify nucleic acid molecules from one or more sources, and / or sequences thereof, with the respective one or more sources. A solution comprising a plurality of probes may be directed across the array to couple at least one of the plurality of probes with at least one of the analytes to form a bound probe. A detector can be configured to detect a signal from the bound probe via scanning of the substrate while minimizing temperature fluctuations of the substrate or optical aberrations caused by bubbles.

The invention discloses a multi-channel high-precision liquid injection device for DNA synthesis and a method thereof, and relates to the field of deoxyribonucleic acid synthesis equipment.The multi-channel high-precision liquid injection device comprises a device shell; the movable mounting box is arranged at the bottom in the device shell in a sliding mode, a mounting groove is formed in the top of the movable mounting box, a clamping plate is arranged at the top in the mounting groove, and a plurality of combined columns are arranged on the clamping plate; and the cover plate is rotationally arranged on the fixed plate through a connecting rod. According to the invention, through the modularized liquid injection head assembly and the flexibly configured sequence identification system, the device can carry out multiple groups of different DNA synthesis operations at the same time, so that the synthesis flux and flexibility are obviously improved; by means of the driving and guiding mechanism and the electromagnetic coupling technology, efficient and accurate positioning of the liquid beating head in a two-dimensional plane is achieved, and the contradiction between multi-channel processing and positioning accuracy is solved.

A sequencing system includes an automated sequencing instrument adapted to determine variant calls for one or more extracted polynucleotide samples with a performance of at least 98.5% raw read accuracy and a run time in a range of 5 hours to 14 hours to determine variant calls for 4 extracted polynucleotide samples using a targeted assay with one DNA pool per sample and an average amplicon size in a range of 100 to 120 bases.

In a method of preparing a flow cell, a slot die coater is used to introduce a nanoparticle suspension at a continuous flow rate to a substrate surface including depressions separated by interstitial regions, which generates a layer of the nanoparticle suspension at a stable concentration across the substrate surface. During the introduction of the nanoparticle suspension, at least some functionalized nanoparticles within the layer respectively enter at least some of the depressions. An excess amount of the layer is removed from the interstitial regions.

A full continuous-flow preparation method of (+)-biotin, including: subjecting a cyclic anhydride and a chiral biphenyl propylene glycol to asymmetric ring-opening reaction to produce a first intermediate, which undergoes selective reduction with a borohydride and cyclization with an inorganic mineral acid to produce (3aS, 6aR)-lactone; subjecting the (3aS, 6aR)-lactone and a sulfenylating reagent to sulfenylation to produce (3aS, 6aR)-thiolactone, which undergoes Fukuyama coupling with a zinc reagent in the presence of a palladium catalyst and elimination reaction in the presence of an inorganic mineral acid to produce an alkenyl valerate compound; subjecting the alkenyl valerate compound to reduction in the presence of a Pd / C catalyst to produce a valerate ester, which undergoes hydrolysis to produce a valeric acid salt; and subjecting the valeric acid salt to debenzylation in the presence of an inorganic mineral acid to produce the target product (+)-biotin.

Provided in some aspects are methods for light-controlled in situ surface patterning of a substrate. Compositions such as nucleic acid arrays produced by the methods are also disclosed. In some embodiments, a method disclosed herein comprises using photoresist for photocontrollable hybridization and / or ligation of nucleic acid molecules, wherein photoresist removal allows hybridization and / or ligation of nucleic acid molecules at the exposed area. A large diversity of barcodes can be created in molecules on the substrate via sequential rounds of light exposure, hybridization, and ligation.

The present application relates to the technical field of pesticide intermediate production, and discloses a continuous chlorination reaction method and equipment for pesticide intermediates, which comprises the following steps: dehydrating and preheating ortho-nitrotoluene raw materials to 60-70 DEG C; continuously injecting a 10-15wt% ferric chloride / dilute hydrochloric acid solution filtered into a reaction system; and feeding the ortho-nitrotoluene pretreated in step a, the ferric chloride solution in step b and dry chlorine into the reaction equipment at flow rates of 8-10 L / h, 1.0-1.5 L / h and 0.8-1.0 m 3 / h respectively, controlling the reaction temperature to be 60-70 DEG C, the system pressure to be 0.4-0.6 MPa and the residence time to be 30-40 min. The present application has the following advantages and effects: full-automatic regulation and control from raw material dehydration to tail gas discharge are realized, the needs of automatic chlorination production are met, efficient mass transfer and heat transfer are realized, a multi-interface mode is adopted, gas-liquid two-phase segmented mixing is realized, the reaction efficiency is improved, the dichloro-substitution side reaction is inhibited through multi-stage temperature control, and the selectivity of monochloro-substitution products is improved.

The invention provides a device for DNA solid-phase synthesis and a preparation method of the device. The device comprises a solid-phase synthesis carrier and a micropore array which is arranged on the solid-phase synthesis carrier and is provided with a wrinkled inner wall. The pore diameter of the micropores is 70-90 [mu] m, the pore depth is 5-6 [mu] m, the inner wall roughness is 0.5-5 [mu] m, the distribution density of the micropores on the surface of the solid-phase synthesis carrier is greater than 1111 / mm < 2 >, and the reaction contact surface provided by the solid-phase synthesis carrier is further expanded; meanwhile, hydrophilic treatment and synthesis starting point grafting are carried out on the inner walls of the micropores, hydrophobic membrane coating is carried out outside the micropores, the synthesis density is increased, and the error rate of the synthesis process is reduced. According to the solid-phase synthesis device, the reaction contact area is further enlarged while the synthesis accuracy is ensured, the coupling density is increased, and a high-yield synthesis device is provided for solid-phase synthesis of DNA by a phosphoramidite method.

In an example of a method of making a flow cell, a functional material is deposited over a resin layer including depressions separated by interstitial regions. The resin layer includes an ultraviolet (UV) light blocking additive. The depressions overlie a first portion of the resin layer having a first thickness, and the interstitial regions overlie a second portion of the resin layer having a second thickness that is greater than the first thickness. The functional material is susceptible to interaction with the resin layer when exposed to UV light. A predetermined UV light dosage is directed through the resin layer, whereby the functional material within the depressions is exposed to the UV light and attaches to the resin layer within the depressions. The functional material overlying the interstitial regions is blocked from being exposed to the UV light by the second resin portion.

Precursors for forming a plurality of multielement materials of different compositions can be deposited on different portions of a common substrate according to a combinatorial approach. The substrate can be subjected to a thermal shock, thereby converting the deposited precursors into separate multielement materials on the substrate. The thermal shock can be a temperature greater than or equal to 500° C. and a duration less than 60 seconds. In some embodiments, each multielement material can be tested with respect to an electrical property, a chemical property, or an optical property. Based on the results of the testing, a composition of a multielement material can be determined for use in a predetermined application, such as use as a catalyst, a plasmonic nanoparticle, an energy storage device, an optoelectronic device, a solid-state electrolyte, or an ion conductive membrane.

Flow cells and methods are described that enable control over DNA fragment length bias during amplification. One example flow cell includes a substrate and a polymeric hydrogel positioned over at least a portion of the substrate. A plurality of oligonucleotide primers may be attached to the polymeric hydrogel, wherein the plurality of oligonucleotide primers defines a density gradient across the substrate of the flow cell.

This invention discloses a method for controllable preparation of nanoparticles using a multi-channel microreactor. The method utilizes a microchannel reactor to segment droplets and prepare quantitatively synthesized nanoparticles with controllable particle size. The invention employs capillary action to draw the reaction solution into the microchannel, segmenting one or more droplets (each droplet into 10-100 portions). By changing the width of the microchannel, controllable preparation of nanoparticles within a small range (±3 nm) can be achieved; by changing the length of the microchannel, controllable preparation of nanoparticles within a large range (±10-30 nm) can be achieved. Furthermore, the reactor structure of this invention is simple, enabling highly precise and quantitative synthesis of various nanoparticles. Compared to large-scale reactors, the miniaturization of the synthesis reaction in this invention effectively reduces energy waste and safety hazards, making it highly valuable for applications in safe chemical engineering, green chemical engineering, high-efficiency chemical engineering, microchemical engineering, and precise reaction monitoring.

The present invention relates to an improved method of a solid-phase phosphoramidite-synthesis for oligonucleotides, preferably RNA molecules having a length of between 50 and 500 nucleotides, as well as to an improved method of purification of said oligonucleotide molecules.

A system for continuous manufacture of biomolecules includes feed tanks suitable for storing products and a reaction chamber designed to be fed with products from the feed tanks that form a reaction phase in the reaction chamber. The reaction chamber is designed to manufacture the biomolecules from the reaction phase. The system includes a scale tank in fluid communication with the reaction chamber, which is suitable for storing an immiscible phase, and a monitoring and control unit set to control the injection flow rate of the immiscible phase into the reaction chamber to keep constant a certain filling level of the reaction chamber according to the amount of reaction phase to be injected into the reaction chamber to manufacture a certain amount of biomolecules.

An example of a flow cell includes a substrate; a first primer set attached to a first region on the substrate, the first primer set including an un-cleavable first primer and a cleavable second primer; and a second primer set attached to a second region on the substrate, the second primer set including a cleavable first primer and an un-cleavable second primer.

The invention is directed to methods and apparatus for parallel enzymatic synthesis of polynucleotides in an array of reaction chambers using a template-free polymerase that has sequence-dependent coupling efficiencies. Whenever sequences causing low efficiency coupling occur at a 3′ end of a growing chain of a polynucleotide being synthesized, one or more additional coupling cycles without de-protection steps are inserted into synthesis plans to provide additional time for completing the coupling reaction at that position of the polynucleotide.

A method comprising receiving a transaction request to print a genetic sequence, the transaction request including an account and a key, a state of the account managed by a blockchain where a plurality of copies of the blockchain include a state of the account; retrieving a stored key associated with the account; authenticating the transaction request based on the stored key and the transaction request key; determining a state of the account in a first copy of the blockchain is the same as in at least a second copy of the blockchain; validating the transaction request based on the determining a state of the account in a first copy of the plurality of copies of the blockchain is the same as in at least a second copy of the plurality of copies of the blockchain; and authorizing the transaction request based on the authentication and validation of the transaction request.

A method for verifying the program code of a synthetic computer program is disclosed. The synthetic computer program is configured to computer-control an automated synthesizer (114) to automatically synthesize at least one oligonucleotide, and the synthetic computer program has a plurality of program cycles for computer-controlling the automated synthesizer (114) to sequentially synthesize oligonucleotides by using at least one sequence of synthesis cycles. The method includes: i. applying an automatic parsing procedure to the program code of the synthetic computer program, the automatic parsing procedure including automatically searching for parameter values of at least one predetermined list of parameters of interest in the program cycles of the synthetic computer program; and ii. automatically constructing a parameter matrix for the program cycles of the synthetic computer program, including the parameters of interest and the corresponding parameter values of the parameters of interest. A computer program and a computer-readable storage medium for executing the method for verifying the program code of a synthetic computer program, a system (110) for verifying the program code of a synthetic computer program, and a method for synthesizing at least one oligonucleotide are further disclosed.

Disclosed are methods and apparatus for synthesizing oligomers, such as oligonucleotides and peptides. The method uses a device including at least one deprotection station for performing a deprotection step, at least one coupling station for performing a coupling step, optionally one or more oxidation and / or thiolation stations for performing an oxidation or thiolation step, optionally one or more capping stations for performing a capping step, and optionally one or more washing stations for performing a washing step. Multiple solid-phase materials for oligomer synthesis are moved to the stations via a conveyor device, where a fluid delivery device acts on the solid-phase materials to produce oligomers.

A miniaturized, automated method for controlled printing of large arrays of nano- to femtoliter droplets by actively transporting mother droplets over hydrophilic-in-hydrophobic (“HIH”) micropatches. The technology uses single or double-plate devices where mother droplets can be actuated and HIH micropatches on one or both plates of the device where the droplets are printed. Due to the selective wettability of the hydrophilic micropatches in a hydrophobic matrix, large nano- to femtoliter droplet arrays are created when mother droplets are transported over the arrays. The parent droplets are moved by various droplet actuation principles. Also, a method using two plates placed one top another while being separated by a spacer. One plate is dedicated to confirming and guiding parent droplets by using hydrophilic patches in a hydrophobic matrix, while the other plate contains HIH arrays for printing of the droplets. When the parent droplet guidance plate is rotated over the plate dedicated to printing of nano- to femtoliter droplets, the droplets are dispensed inside the HIH array utilizing their selective wettability. The methods allow the parent droplets to move over the HIH arrays many times, providing advantages for performing bio-assays or miniaturized materials synthesis in nano- to femtoliter sized droplets. With controlled evaporation of the dispensed droplets of solution, large arrays of printed material can be generated in seconds. The methods provide a nano- to femtoliter droplet printing technique for a wide variety of applications, e.g., protein- or cell-based bio-assays or printing of crystalline structures, suspensions of nanoparticles or microelectronic components.

A polymeric hydrogel is applied to a surface of a substrate including depressions separated by interstitial regions. Each depression includes a UV blocking layer positioned at a first region, and a second region that is transparent to UV and adjacent to the first region. Before or after the hydrogel is applied, a primer set is grafted to the hydrogel. The primer set includes 3' blocked uncleavable first and second pre-primers. The hydrogel or grafted layer is removed from the interstitial regions. At a first predetermined region of the grafted layer within the second region: the first pre-primers are altered to introduce cleavable first primers; and the second pre-primers are altered to introduce uncleavable second primers. At a second predetermined region of the grafted layer within the first region: the first pre-primers are altered to introduce uncleavable first primers; and the second pre-primers are blocked to introduce cleavable second primers.

This application relates to functionalized magnetic particles and methods of using magnetic particles to generate amplicons. In some examples, a method of modifying a magnetic particle comprising a first functional group includes contacting the magnetic article with a first molecule comprising a polymer coupled to second and third functional groups, wherein the first functional group reacts with the second functional group to form a bond via which the polymer is coupled to the magnetic particle. The method may include contacting the magnetic particle with a second molecule, the second molecule comprising an oligonucleotide coupled to a fourth functional group, wherein the third functional group reacts with the fourth functional group to form a bond via which the ligonucleotide is coupled to the magnetic particle.

Embodiments disclosed herein are directed to microfluidic devices that allow for scalable on-chip screening of combinatorial libraries and methods of use thereof. Droplets comprising individual molecular species to be screened are loaded onto the microfluidic device. The droplets are labeled by methods known in the art, including but not limited to barcoding, such that the molecular species in each droplet can be uniquely identified. The device randomly sorts the droplets into individual microwells of an array of microwells designed to hold a certain number of individual droplets in order to derive combinations of the various molecular species. The paired droplets are then merged in parallel to form merged droplets in each microwell, thereby avoiding issues associated with single stream merging. Each microwell is then scanned, e.g., using microscopy, such as high content imaging microscopy, to detect the optical labels, thereby identifying the combination of molecular species in each microwell.

Compositions, systems, and methods for the display of analytes such as biomolecules are described. Display of analytes is achieved by coupling of the analytes to displaying molecules that are configured to associate with surfaces or interfaces. Arrays of analytes may be formed from the described systems for utilization in assays and other methods.

To provide a method for determining a nucleic acid sequence, capable of reducing a non-specific background signal, thereby improving a contrast noise ratio (CNR), thereby improving accuracy of base calling and potentially reducing a cycle period, and reducing dependency to an accurate optical system and expensive equipment for a sequence determination process.SOLUTION: A method comprises: a) a step for providing a coupled surface to at least one polymer layer including a polymer coupled to a first nucleic acid molecule; b) a step for bringing the first nucleic acid molecule into contact with a second nucleic acid molecule so that the second nucleic acid molecule is coupled to the first nucleic acid molecule; c) a step for, after step b), magnifying the second nucleic acid molecule or a derivative thereof; and d) a step for acquiring a fluorescent image of the surface.SELECTED DRAWING: Figure 3

Provided are microfluidic devices. The devices comprise a chamber configured to contain a liquid medium. The devices further comprise a substrate comprising: i) a photoconductive layer forming a first wall of the chamber; and ii) first and second electrodes disposed in a planar arrangement within or adjacent to the photoconductive layer. The devices further comprise a microfluidic inlet channel connected to the chamber, and a microfluidic droplet generator connected to the chamber. Systems comprising the microfluidic devices of the present disclosure are also provided. The systems comprise a light pattern generator optically coupled to the photoconductive layer; and a voltage source electrically coupled to the first and second electrodes. Methods of engulfing a particle into a droplet implemented using a system of the present disclosure are also provided.

An example of a flow cell includes a substrate having an active region that is directly adjacent to an inactive region. The active region includes a hydrophilic material disposed within a plurality of first depressions that are separated by first interstitial regions. The inactive region includes a fluidic pinning region that is directly adjacent to the active region. The inactive region further includes a plurality of second depressions that are separated by second interstitial regions, wherein at least some of the plurality of second depressions are directly adjacent to the fluidic pinning region. The inactive region still further includes a hydrophobic barrier positioned over at least the plurality of second depressions. The hydrophobic barrier includes a hydrophobic polymer.