394results about "Sequential/parallel process reactions" patented technology

A preparation equipment and a process for a nylon salt solution. The preparation equipment includes a suspension preparation device, a first salt formation reactor and a second salt formation reactor. The suspension preparation device includes a feeding unit, a continuous feeding unit, a high-shear pump and a mixing reactor, the high-shear pump and the mixing reactor are cyclically connected with and in communication with each other through two connecting pipelines; the second salt formation reactor includes a second diamine feed pipe, a third diamine feed pipe, a circulation pipeline of the second salt formation reactor and an online near-infrared monitoring equipment located on the circulation pipeline of the second salt formation reactor.

The invention relates to a preparation method and a preparation device of isocyanate, and the preparation method comprises the following steps: carrying out cold reaction on a phosgene solution and an amine solution in a cold reactor to obtain an intermediate mixed solution; separating the intermediate mixed solution by adopting a cyclone, obtaining a carbamoyl chloride mixed solution from an underflow port of the cyclone, and obtaining an amine hydrochloride mixed solution from an overflow port of the cyclone; the carbamyl chloride mixed solution enters a first thermal reactor for thermal reaction to obtain a first reaction product, and the amine hydrochloride mixed solution enters a second thermal reactor for thermal reaction to obtain a second reaction product; mixing the first reaction product and the second reaction product in a staying reactor to obtain an isocyanate reaction solution; and carrying out post-treatment on the isocyanate reaction liquid to obtain the isocyanate. The method can reduce the generation amount of urea by-products, further inhibits the generation of other subsequent by-products, reduces the chromaticity of the product, and improves the quality of isocyanate.

A Solid Phase Peptide Synthesis (SPPS) device and method of using the same for manufacturing peptides is taught herein. The system comprises at least two reactors, each reactor including a quantity of SPPS resin. The reactors are positioned in series. A de-protecting agent is added to the first reactor and then transferred to the second and third reactors, in series, thereby operating to de-protect the protected N-group. Wash solvent is added to the first reactor and then transferred to the second and this operation repeated several times. Likewise, an amino acid activated ester solution is added, in series, to the first, second and third reactors, thereby operating to couple the amino acid to the de-protected N-group. Wash solvent is added to the first reactor and then transferred to the second and this operation repeated several times prior to the next cycle. The use of the reactors in series reduces the overall solvent required. Online LCMS is also used to monitor progress and identity of reactions happening within the solid phase resin particles.

The invention provides an inlet valve array, a cleaning method and a biosynthesis system based on the inlet valve array. A first connecting flow channel and a second connecting flow channel of the inlet valve array are connected with a cleaning main pipeline through pipelines; outlets of the first connecting flow channels of the two inlet valve arrays are connected with a first liquid inlet main pipeline through pipelines. A first connecting flow channel of one inlet valve array is connected with a second liquid inlet main pipeline through a pipeline, the first liquid inlet main pipeline and the second liquid inlet main pipeline are respectively connected with a column front input pipeline, and the column front input pipeline is connected with the input ends of a plurality of composite columns through a plurality of valves; an output end pipeline of each composite column is connected with an inlet of a post output pipeline through a corresponding valve; an outlet of the post-column output pipeline is connected with a discharge pipeline and a second liquid inlet main pipeline through valves; the device has the advantages that cross contamination generated when different materials are conveyed can be avoided, the synthesis purity is improved, and waste of the materials is reduced, so that the cost is reduced; and the pump can be switched without stopping.

The invention is directed to methods for massively parallel template-free enzymatic synthesis of a plurality of different polynucleotides of predetermined sequences. In one aspect, methods of the invention employ large scale arrays of reaction sites each associated with at least one working electrode for controlling deprotection and deblocking steps at predetermined user selected sites. In another aspect, the invention provides template-free enzymatic synthesis with proofreading, wherein completed polynucleotides at predetermined reaction sites are sequenced using a sequencing by synthesis technique, particularly employing electrochemically labile blocking groups.

Provided are systems and methods for analyte detection and analysis. A system can comprise an open substrate. The open substrate may be configured to rotate or otherwise move. The open substrate can comprise an array of individually addressable locations, with analytes immobilized thereto. The substrate may be spatially indexed to identify nucleic acid molecules from one or more sources, and / or sequences thereof, with the respective one or more sources. A solution comprising a plurality of probes may be directed across the array to couple at least one of the plurality of probes with at least one of the analytes to form a bound probe. A detector can be configured to detect a signal from the bound probe via scanning of the substrate while minimizing temperature fluctuations of the substrate or optical aberrations caused by bubbles.

The electronic-grade silane gas continuous production preparation system comprises a cold hydrogenation treatment device, an inlet of the cold hydrogenation treatment device is connected with a raw material supply system, and an outlet of the cold hydrogenation treatment device is connected with a cold hydrogenation rectifying tower through a pipeline. The cold hydrogenation rectifying tower is sequentially connected with the boron and phosphorus removal adsorption column, the first-stage disproportionator, the first tower, the second tower, the second-stage disproportionator, the third tower and the fourth tower through pipelines, and electronic-grade silane in the fourth tower is used for being conveyed to a silane tank area; the first tower is used for removing silicon tetrachloride produced after the disproportionation reaction of the first-stage disproportionator; the second tower is used for separating the extracted chlorosilane material; the third tower is used for separating silane from chlorosilane; and the fourth tower is used for discharging light impurities and heavy impurities and conveying gas-phase electronic-grade silane into a silane tank area. The device and the method realize continuous, efficient and high-purity production of the electronic-grade silane gas, and have the advantages of low energy consumption, low cost, thorough impurity removal and the like.

The application discloses a gene chip and a gene chip detection device. The gene chip comprises a substrate and a positioning device fixed to the upper surface of the substrate; the positioning device is provided with a containing cavity used for containing microbeads; the containing cavity is opened on the surface of the positioning device away from the substrate; and the area of the containing cavity is gradually narrowed in the direction of the height of the positioning device towards the upper surface of the substrate. The gene detection device comprises the gene chip and the microbeads covered with fluorescent probes, and the microbeads are clamped in the containing cavity. The gene chip and the gene chip detection device of the application reduce the reflection of the surface of the containing cavity of the substrate to excitation light, and at the same time, the exposed area of the microbeads is increased. In the process of using the gene chip, the proportion of the fluorescence excited by the fluorescent probes on the microbeads in the light received by the detection device is increased, so that the accuracy of the gene chip detection is improved.

The invention discloses a multi-channel high-precision liquid injection device for DNA synthesis and a method thereof, and relates to the field of deoxyribonucleic acid synthesis equipment.The multi-channel high-precision liquid injection device comprises a device shell; the movable mounting box is arranged at the bottom in the device shell in a sliding mode, a mounting groove is formed in the top of the movable mounting box, a clamping plate is arranged at the top in the mounting groove, and a plurality of combined columns are arranged on the clamping plate; and the cover plate is rotationally arranged on the fixed plate through a connecting rod. According to the invention, through the modularized liquid injection head assembly and the flexibly configured sequence identification system, the device can carry out multiple groups of different DNA synthesis operations at the same time, so that the synthesis flux and flexibility are obviously improved; by means of the driving and guiding mechanism and the electromagnetic coupling technology, efficient and accurate positioning of the liquid beating head in a two-dimensional plane is achieved, and the contradiction between multi-channel processing and positioning accuracy is solved.

The present disclosure relates to processes for suspending stretched nucleic acids over surface features. These processes can be used to prepare stretched nucleic acids that are more active in enzymatic reactions and other reactions than those laid down on a flat surface. These processes can be achieved by using a photoresist layer on top of a substrate, stretch a nucleic acid on top of the surface, and then remove part of the photoresist to form surface features that suspend the stretched nucleic acid. Furthermore, the formation of a hydrogel layer over the stretched nucleic acid and the surface features can transfer the stretched nucleic acid to the hydrogel for further reactions, including enzymatic reactions.

A sequencing system includes an automated sequencing instrument adapted to determine variant calls for one or more extracted polynucleotide samples with a performance of at least 98.5% raw read accuracy and a run time in a range of 5 hours to 14 hours to determine variant calls for 4 extracted polynucleotide samples using a targeted assay with one DNA pool per sample and an average amplicon size in a range of 100 to 120 bases.

In a method of preparing a flow cell, a slot die coater is used to introduce a nanoparticle suspension at a continuous flow rate to a substrate surface including depressions separated by interstitial regions, which generates a layer of the nanoparticle suspension at a stable concentration across the substrate surface. During the introduction of the nanoparticle suspension, at least some functionalized nanoparticles within the layer respectively enter at least some of the depressions. An excess amount of the layer is removed from the interstitial regions.

A full continuous-flow preparation method of (+)-biotin, including: subjecting a cyclic anhydride and a chiral biphenyl propylene glycol to asymmetric ring-opening reaction to produce a first intermediate, which undergoes selective reduction with a borohydride and cyclization with an inorganic mineral acid to produce (3aS, 6aR)-lactone; subjecting the (3aS, 6aR)-lactone and a sulfenylating reagent to sulfenylation to produce (3aS, 6aR)-thiolactone, which undergoes Fukuyama coupling with a zinc reagent in the presence of a palladium catalyst and elimination reaction in the presence of an inorganic mineral acid to produce an alkenyl valerate compound; subjecting the alkenyl valerate compound to reduction in the presence of a Pd / C catalyst to produce a valerate ester, which undergoes hydrolysis to produce a valeric acid salt; and subjecting the valeric acid salt to debenzylation in the presence of an inorganic mineral acid to produce the target product (+)-biotin.

The invention relates to an arsenic pentafluoride preparation device and method. The preparation device comprises a fluorine gas collecting tank, a first reactor, a second reactor, a rectifying tower, a heat exchanger, an arsenic trifluoride collecting steel cylinder, a product collecting steel cylinder, a first-stage adsorption tower and a second-stage adsorption tower. A fluorine gas collecting tank is connected with a first reactor and a second reactor, an outlet of the first reactor is connected with the second reactor, an outlet of the second reactor is connected with a rectifying tower, and a first outlet and a second outlet of the rectifying tower are connected with an arsenic trifluoride collecting steel cylinder and a heat exchanger respectively. A first outlet of the heat exchanger is connected with a second inlet of the rectifying tower and a first inlet of the product collecting steel cylinder, a second outlet of the heat exchanger is connected with a second inlet of the product collecting steel cylinder, the product collecting steel cylinder is connected with the first-stage adsorption tower, and the first-stage adsorption tower is further connected with the second-stage adsorption tower. The device is simple, the two-stage reactor is adopted to inhibit generation of byproducts, high-temperature and high-pressure conditions are not needed, the fluorine gas utilization rate and the arsenic conversion rate are increased, and high-purity arsenic pentafluoride can be prepared.

Provided in some aspects are methods for light-controlled in situ surface patterning of a substrate. Compositions such as nucleic acid arrays produced by the methods are also disclosed. In some embodiments, a method disclosed herein comprises using photoresist for photocontrollable hybridization and / or ligation of nucleic acid molecules, wherein photoresist removal allows hybridization and / or ligation of nucleic acid molecules at the exposed area. A large diversity of barcodes can be created in molecules on the substrate via sequential rounds of light exposure, hybridization, and ligation.

Methodology and apparatus for on-line UV monitoring of automated synthesis reactions. An apparatus includes a module with a fluidic cell the operational parameters of which remain substantially unchangeable not only during the process of monitoring but also in time between sequential processes. The module includes a separable housing structure containing a source of UV-light and an optical detector integrated with substantially temperature-insensitive fluidic cell. A portion of the cell is defined by a slot formed in a cell-chassis and complemented with inlet and outlet dimensioned to ensure that no air-bubble(s) and / or stagnating fluid is present in the cell during time when liquid reactants to-be-measured are delivered through the inlet into the cell. The method is configured to determine progression and completion of the reaction(s) and modification of reactions' times and repetitions of reaction(s)s in real time.

The present application relates to the technical field of pesticide intermediate production, and discloses a continuous chlorination reaction method and equipment for pesticide intermediates, which comprises the following steps: dehydrating and preheating ortho-nitrotoluene raw materials to 60-70 DEG C; continuously injecting a 10-15wt% ferric chloride / dilute hydrochloric acid solution filtered into a reaction system; and feeding the ortho-nitrotoluene pretreated in step a, the ferric chloride solution in step b and dry chlorine into the reaction equipment at flow rates of 8-10 L / h, 1.0-1.5 L / h and 0.8-1.0 m 3 / h respectively, controlling the reaction temperature to be 60-70 DEG C, the system pressure to be 0.4-0.6 MPa and the residence time to be 30-40 min. The present application has the following advantages and effects: full-automatic regulation and control from raw material dehydration to tail gas discharge are realized, the needs of automatic chlorination production are met, efficient mass transfer and heat transfer are realized, a multi-interface mode is adopted, gas-liquid two-phase segmented mixing is realized, the reaction efficiency is improved, the dichloro-substitution side reaction is inhibited through multi-stage temperature control, and the selectivity of monochloro-substitution products is improved.

The invention relates to a method for microarray transformation, wherein, by using a cavity chip with transformation matrix, a template array can be copied onto a planar support, and the information or spatial arrangement is changed in the process, so that a transformed second array forms. The invention further relates to a device for carrying out such a method.

Compositions, methods, and systems for handling liquids, for example, liquids containing one or more biological substances or chemical reagents, or combinations thereof. The liquid can be an "ink" containing nucleic acids (e.g., DNA) for "nucleic acid "printing" or "writing." These nucleic acids can include one or more components of nucleic acid molecules that encode digital information ("identifiers") that can be used in methods and systems for encoding digital information in nucleic acid. Described in this specification are technologies including an ink supply system and associated ecosystem for a DNA writing device for use with the technologies for encoding, storing, reading, and / or processing digital information in nucleic acid molecules.

The invention provides a device for DNA solid-phase synthesis and a preparation method of the device. The device comprises a solid-phase synthesis carrier and a micropore array which is arranged on the solid-phase synthesis carrier and is provided with a wrinkled inner wall. The pore diameter of the micropores is 70-90 [mu] m, the pore depth is 5-6 [mu] m, the inner wall roughness is 0.5-5 [mu] m, the distribution density of the micropores on the surface of the solid-phase synthesis carrier is greater than 1111 / mm < 2 >, and the reaction contact surface provided by the solid-phase synthesis carrier is further expanded; meanwhile, hydrophilic treatment and synthesis starting point grafting are carried out on the inner walls of the micropores, hydrophobic membrane coating is carried out outside the micropores, the synthesis density is increased, and the error rate of the synthesis process is reduced. According to the solid-phase synthesis device, the reaction contact area is further enlarged while the synthesis accuracy is ensured, the coupling density is increased, and a high-yield synthesis device is provided for solid-phase synthesis of DNA by a phosphoramidite method.

In an example of a method of making a flow cell, a functional material is deposited over a resin layer including depressions separated by interstitial regions. The resin layer includes an ultraviolet (UV) light blocking additive. The depressions overlie a first portion of the resin layer having a first thickness, and the interstitial regions overlie a second portion of the resin layer having a second thickness that is greater than the first thickness. The functional material is susceptible to interaction with the resin layer when exposed to UV light. A predetermined UV light dosage is directed through the resin layer, whereby the functional material within the depressions is exposed to the UV light and attaches to the resin layer within the depressions. The functional material overlying the interstitial regions is blocked from being exposed to the UV light by the second resin portion.

Precursors for forming a plurality of multielement materials of different compositions can be deposited on different portions of a common substrate according to a combinatorial approach. The substrate can be subjected to a thermal shock, thereby converting the deposited precursors into separate multielement materials on the substrate. The thermal shock can be a temperature greater than or equal to 500° C. and a duration less than 60 seconds. In some embodiments, each multielement material can be tested with respect to an electrical property, a chemical property, or an optical property. Based on the results of the testing, a composition of a multielement material can be determined for use in a predetermined application, such as use as a catalyst, a plasmonic nanoparticle, an energy storage device, an optoelectronic device, a solid-state electrolyte, or an ion conductive membrane.

Flow cells and methods are described that enable control over DNA fragment length bias during amplification. One example flow cell includes a substrate and a polymeric hydrogel positioned over at least a portion of the substrate. A plurality of oligonucleotide primers may be attached to the polymeric hydrogel, wherein the plurality of oligonucleotide primers defines a density gradient across the substrate of the flow cell.

Apparatuses and a method for plate-based oligonucleotide synthesis are disclosed. In one example, an apparatus used in oligonucleotide synthesis includes a synthesis adapter base to receive a commercially available synthesis plate. A keeper is used to apply pressure to the commercially available synthesis plate, and a sealing element is used to seal the commercially-available synthesis plate to the system adapter base. Other methods and apparatuses are disclosed.

This invention discloses a method for controllable preparation of nanoparticles using a multi-channel microreactor. The method utilizes a microchannel reactor to segment droplets and prepare quantitatively synthesized nanoparticles with controllable particle size. The invention employs capillary action to draw the reaction solution into the microchannel, segmenting one or more droplets (each droplet into 10-100 portions). By changing the width of the microchannel, controllable preparation of nanoparticles within a small range (±3 nm) can be achieved; by changing the length of the microchannel, controllable preparation of nanoparticles within a large range (±10-30 nm) can be achieved. Furthermore, the reactor structure of this invention is simple, enabling highly precise and quantitative synthesis of various nanoparticles. Compared to large-scale reactors, the miniaturization of the synthesis reaction in this invention effectively reduces energy waste and safety hazards, making it highly valuable for applications in safe chemical engineering, green chemical engineering, high-efficiency chemical engineering, microchemical engineering, and precise reaction monitoring.

The present disclosure relates in some aspects to methods for manufacturing molecular arrays using a hybrid approach comprising microfluidics-based delivery and photolithographyguided oligonucleotide hybridization and ligation. In particular, the molecular arrays can be used for determining spatial patterns of abundance and / or expression of a biological target in a sample.