48 results about "Genetic element" patented technology

Most therapeutic treatments require dose adjustment after initial administration. Dosage adjustment is crucial to ensuring optimal safety and efficacy characteristics of the treatment. Gene therapy is inherently limited by the inability to adjust the dose after initial administration. This invention describes a genetic element capable of dose adjustment after the initial administration of gene therapy. The realization of dose adjustment allows for the replacement of multiple subcutaneous protein injection regimens with single-injection gene therapy, thereby improving pharmacokinetics, safety / efficacy characteristics, medication adherence, and reducing healthcare costs.

This technology provides targeted genome editing techniques for altering the color of parts of grape plants and their fruits. Specifically, this technology relates to using genome editing methods to generate edits in target genes that cause large fragment inactivation deletions, thereby altering the color traits of plants and fruits. These target genes include genes and genetic elements associated with plant color and pigment deposition (e.g., the PDS1 gene, the mybA1 gene, and the Gret1 retrotransposon).

Disclosed are variants of Fas ligands (APTL, FASL, CD178, CD95L, ALPS1B, CD95-L, TNFSF6, TNLG1A, APT1LG1) and vectors providing them. Furthermore, recombinant cells, such as NK cells (natural killer cells), containing genetic elements that enable the production of at least one FasL variant to enhance the cytotoxicity and viability of recombinant cells and improve the therapeutic activity of recombinant cells are disclosed. Also disclosed are pharmaceutical compositions containing recombinant cells and methods for obtaining them, as well as NK cells modified to express Fas ligand variants for use in immunotherapy.

Gene therapy compositions and methods related to transposition are provided, e.g., those engineered from Myotis myotis.

Methods of evaluating microorganisms to determine efficiency of colonization of a plant or plant part are described. Methods of identifying genetic elements correlated with colonization efficiency of plant-associated microorganism are also provided. Methods to identify microorganisms for use as inoculants for improved plant yield using the colonization screening methods or the presence of genetic elements associated with colonization efficiency are described. Further methods useful for identification of microorganisms useful as inoculants for improving plant yield are presented.

A geminivirus replicon, an in planta directed evolution / selection system based thereon, and use. Provided is a geminivirus-based controllable artificial replicon, which comprises two LIRs derived from BeYDV; the two LIRs are located at two flanks of the artificial replicon, respectively. Also provided is an in planta directed evolution / selection system, which comprises the geminivirus-based controllable artificial replicon, a Rep and / or RepA protein, and a mutant or mutant library of a genetic element. The replication of the geminivirus-based controllable artificial replicon is configured to be associated with the desired function of the mutant of the genetic element. The provided in planta directed evolution / selection system is a universal, rapid, and efficient in planta directed evolution system, and can be used for generating new excellent alleles and assisting future agricultural breeding.

The invention relates to a microbial strain DNA (deoxyribonucleic acid) mixed coding and an alternating Transformer depth prediction method for gene expression of the microbial strain DNA mixed coding, and belongs to the crossing field of bioinformatics and artificial intelligence. The core of the method is to construct a deep learning model capable of efficiently capturing DNA sequence characteristics, and end-to-end accurate prediction of the expression level of a target gene in a specific microbial host is realized. The method comprises the following steps: carrying out binary coding on four conventional basic groups of DNA of a target strain, splicing eight key biological characteristics closely related to gene expression, and carrying out hybrid coding to generate a combined characteristic matrix; a deep learning network containing an alternating Transform coding structure is established, local structure information of a DNA combination feature matrix and a global context dependency relationship are deeply fused, and the analysis precision of microorganism DNA sequence data and the prediction accuracy of a gene expression level are improved. According to the technical scheme, an efficient and accurate computer-aided prediction tool is provided for optimization design of genetic elements (such as promoters) and gene sequences, the expensive experiment trial and error cost and the strain characterization cost can be remarkably reduced, and the research and development process of high-yield strains is accelerated.

Mobile genetic elements or cells that are resistant to horizontal gene transfer, and methods for obtaining the cells, are provided. Also provided are methods for suppressing horizontal transfer of genetic information between a mobile genetic element and a first cell, cells utilizing novel gene codon schemes and related subject matter, and kits containing cells and mobile genetic elements that are orthogonal to one another. Also provided are methods for altering the susceptibility of a gene to mutations that alter the encoded amino acid sequence, methods for evolving or improving proteins, and methods for making a target gene more resistant to mutations. Additionally, the use of cells to make polymers and methods comprising using cells to make polymers are provided.

The present invention describes recombinant constructs, cells, and devices for increasing the production of adeno-associated virus (AAV). The present invention also describes methods for producing recombinant AAV using the constructs and the cells.

The present invention relates generally to methods and materials for boosting gene expression in eukaryotic cells, for example the expression of heterologous genes encoding proteins of interest. In particular, it relates to gene expression cassettes with increased expression rates.

Provided are lipid formulations capable of forming lipid-based nanoparticles comprising a molar ratio of ionizable lipid to phospholipid of 0.1 to 1.30, wherein the lipid formulation is associated with a nucleic acid payload and, in some embodiments, a stabilizer. In some embodiments, the nucleic acid payload is a vaccine genetic element.

Described herein are optimally-modified GJB2 cDNA and associated genetic elements for use in recombinant adeno-associated virus (rAAV)-based gene therapy for the treatment of genetic hearing loss.

Provided are cells that are resistant to mobile genetic elements or horizontal gene transfer, and methods for obtaining said cells. Also provided are methods for preventing the horizontal transfer of genetic information between a mobile genetic element and a first cell, cells making use of new genetic codons schemes and related subject matter, kits comprising mutually orthogonal cells, and mobile genetic elements. Also provided are methods of altering the susceptibility of a gene to mutations that alter the encoded amino acid sequence, methods for evolving or improving a protein, and methods for rendering a target gene more resistant to mutation. Additionally provided are uses of the cells for making polymers and methods comprising using the cells for making polymers.

Here, the inventors use genomics to discover a new family of lanthipeptides from Actinobacteria dedicated to anti-phage defense. They demonstrate that a specific family of metabolic pathways that produce unknown class I lanthipeptides are encoded near known anti-phage defense systems or within mobile genetic elements, indicating their anti-phage function. Furthermore, the inventors show that the heterologous expression of these pathways in Streptomyces albus (a model strain of Actinobacteria) confers anti-phage defense. The experimental results show that different lanthipeptides of this family confer a selective protection against phages, without compromising the cell viability. The inventors also have identified a specific regulatory system that controls the expression of "defensive lanthipeptides" in Actinobacteria and use this to demonstrate their anti-phage function in a native strain. Finally, the inventors explore the anti-phage mechanism of action of these compounds. Thus the present invention relates to a new class of anti-phage natural peptides.

A method of producing an antibody specific for a target antigen, the method comprising (a) immunizing an animal one or more rounds with the target antigen; (b) collecting a first sample from B cells of the animal; (c) identifying one or more first antibodies specifically binding to the antigen from the B cells in the first sample, and determining a genetic element characterizing the B cell lineage of the B cells in the first sample; (d) performing one or more additional rounds of immunization on the animal with the target antigen; (e) collecting a second sample from the B cells of the animal; (f) identifying one or more second samples from the B cells of the second sample, the B cells of the second sample being from the same cell lineage as the B cells from the first sample; and (g) testing the specific binding of the second antibody to a target antigen. Optionally, in certain embodiments, steps (d) to (g) are repeated.

Cells resistant to moving genetic elements or horizontal gene transfer, as well as methods for obtaining the cells, are provided. Also provided are methods for preventing horizontal transfer of genetic information between a mobile genetic element and a first cell, cells utilizing the new genetic codon regimen and related subject matter, kits comprising mutually orthogonal cells, and mobile genetic elements. Also provided are methods of altering the susceptibility of a gene to altering mutations of an encoded amino acid sequence, methods for evolving or improving a protein, and methods for making a target gene more resistant to mutations. Also provided are uses of the cells for making polymers and methods comprising making polymers using the cells.

The invention provides a method for identifying, quantifying and visualizing genomes of drug-resistant pathogenic bacteria in environmental samples, which is suitable for identifying dangerous drug-resistant pathogenic bacteria in various environments. The method comprises the following steps: (1) based on a metagenome sequencing big data set, obtaining a high-quality bacterial genome by using genome quality control, assembly and binning technologies; (2) identifying a drug-resistant bacterium genome carrying an antibiotic drug-resistant gene, further annotating a virulence factor, and comparing with an authoritative pathogenic bacterium directory to realize accurate identification of drug-resistant pathogenic bacteria; and (3) mapping the reference genome of the drug-resistant pathogenic bacteria through the metagenome so as to quantify the abundance of the drug-resistant pathogenic bacteria, and further carrying out visual analysis on the drug-resistant pathogenic bacteria mediated drug-resistant genes, related movable genetic elements and other gene neighborhood structures. The method does not depend on isolated culture, realizes full-spectrum analysis of drug-resistant pathogenic bacteria in environmental samples, and provides important technical support for accurate and scientific evaluation of drug resistance risks of environmental bacteria antibiotics.

The application belongs to the technical field of biomedicine, and provides an alginate-based thermal genetics CXCL12 releaser, a preparation method and application thereof. The application uses transgenic and freeze-drying technology to prepare an alginate-based thermal genetics CXCL12 releaser (ATCG) based on an optimized alternating magnetic field. The preparation is convenient and simple, and can be produced on a large scale. The ATCG is composed of a porous magnetic support loaded with engineering cells. The engineering cells are stably transfected with a pHSP70-CXCL12 thermal genetics element by a lentivirus. The porous magnetic support is composed of a main frame structure of a biopolymer material wrapped with magnetic particles, and has an isotropic porous structure. The ATCG prepared by the application can controllably release CXCL12 chemotactic factors for DTC recruitment, and can activate the anti-DTC peritoneal immunity after magnetic heat ablation, thereby inhibiting the peritoneal metastasis of DTC.

Described herein are methods for modulating the expression of complex gene networks by increasing expression of an RNA-encoding therapeutic genetic element that is normally attenuated in response to an internal or environmental signal while simultaneously disrupting expression of an endogenous RNA-encoding sequence that is normally activated in response to an internal or environmental signal. Methods for enhancing cell therapies and treatment of conditions related to aberrant gene expression are also described.

The present invention discloses a method for detecting mobile genetic elements based on whole-genome data. The method uses an MGE feature sequence extraction algorithm to obtain the feature sequences of mobile genetic elements, and obtains a predicted set and a result set of mobile genetic elements through the blast tool. The whole-genome sequencing technology is used to perform individual analysis and determination of the complete gene sequence information of bacteria with unknown genomic sequences, effectively solving the problem that the mobile genetic element database cannot be updated in time, resulting in the inability to detect all mobile genetic elements. Furthermore, the detection rate of mobile elements is improved, and a unified format is provided for the naming of the mobile genetic element database to prevent the same mobile genetic element from being misidentified as different mobile genetic elements.

Vectors, cell lines comprising the vectors, recombinant virions produced from the vectors, and methods of using the vectors for barcoding nuclei of cells are provided. Vector libraries that collectively encode a plurality of genetic elements of interest for screening and methods of screening cells having nuclei barcoded according to the subject methods are also provided.

The invention discloses an editor fusing 5 'exonuclease and CRISPR-Cas12i2 as well as construction and application of the editor, and relates to the technical field of plant biology. The editor for fusing the 5'exonuclease and the CRISPR-Cas12i2 comprises a 5 'exonuclease T5E and Cas12i2 variant fusion protein T5E-Cas12i2 v1 coded by a nucleotide sequence, and a nuclear localization signal bpNLS, the 5' exonuclease T5E, the Cas12i2 variant Cas12i2 v1 and a nuclear localization signal bpNLS are sequentially arranged from the N end to the C end. Compared with an existing plant genome editing tool, the constructed T5E-Cas12i2v1 editor has the advantages that the efficiency is high, TTN-PAM sites are targeted, and site-specific large fragment deletion can be generated. The method is beneficial to accurate manipulation research on large cis-genetic elements, and has a wide application prospect in plant functional genome research and genetic improvement of crops.

This disclosure provides methods for performing quantitative-trait loci (QTL) analysis in bacteria. The methods of the instant disclosure utilize multiple rounds of protoplast fusion-induced genomic recombination to break genetic linkages in bacterial genomes. The methods of the instant disclosure allow determining which genetic elements (QTL) are associated with phenotypic al features.

This invention relates to GM002714 The application of genes, recombinant vectors containing these genes, and recombinant bacteria in increasing the yield of Streptomyces natural products belongs to the field of genetic engineering technology. To discover and utilize yield-related genetic elements in the Streptomyces metabolic network to improve the yield of Streptomyces natural products, this invention utilizes transposon mutagenesis technology to discover and elucidate for the first time in Streptomyces the function of a two-component regulatory system involved in citric acid metabolism—TCS2713. By constructing a recombinant vector and recombinant bacteria containing the gene encoding the transcriptional regulator GM002714 of this two-component regulatory system, overexpression was observed in *Streptomyces avermitilis*, *Streptomyces cerevisiae*, *Streptomyces glabripennis*, or *Streptomyces roseospora*. GM002714 Genes can increase the yield of natural products. This invention provides valuable biosynthetic elements for the efficient biomanufacturing of Streptomyces.

This invention belongs to the field of solid waste resource utilization technology, and relates to a method for synergistic reduction of antibiotic resistance genes in anaerobic digester residues through hydrothermal-induced iron-calcium-magnesium-silicon-aluminum mineralization. Anaerobic digester residues are used as the treatment target. After screening, homogenization, and solids content adjustment, a composite mineralization inducer is added and the pH is adjusted, and a mineralization reaction is carried out under closed hydrothermal conditions. During the reaction, the hydrothermal effect promotes the rupture of resistant bacterial cells, the deconstruction of extracellular polymers, and DNA strand breaks in the digester residues. Simultaneously, iron, calcium, magnesium, silicon, and aluminum components undergo in-situ mineralization with phosphates, carbonates, humic substances, and dissolved organic matter in the digester residues, forming a composite mineral phase. This complexes, precipitates, encapsulates, and passivates antibiotic resistance gene fragments, mobile genetic elements, extracellular DNA, antibiotic residues, and heavy metals. This method achieves synergistic reduction of resistance genes, decrease in migration risk, weakening of selection pressure, and resource utilization of digester residues, and has advantages such as short treatment cycle, high reduction efficiency, good product stability, and strong engineering applicability.

The application discloses a genetic element for enhancing lactic acid metabolic flux of actinomycetes, and belongs to the field of synthetic biology and microbial metabolic engineering. sucC The application successfully constructs a single expression element and a co-expression element by cloning genes from Streptomyces coelicolor sucD , and verifies that the SucC protein and the SucD protein both have lactic acid coenzyme A synthetase activity through in-vitro protein purification and enzyme activity determination, and the enzyme activity of a protein complex (SucC-SucD, SucCD) expressed by the co-expression element is significantly higher than that of the single protein, indicating that the two proteins have a synergistic effect; the genetic element can efficiently catalyze the combination reaction of lactic acid and coenzyme A in vitro, accelerates the lactic acid metabolic speed, provides a core genetic element for the modification of the lactic acid metabolic pathway of actinomycetes, enriches a synthetic biology tool library, and has important industrial application prospects and academic value.

The present application belongs to the technical field of excrement pollution prevention and treatment, and relates to a pollutant removal promoter and application thereof. The present application uses fresh earthworm body fluid and earthworm manure mixture as raw material of the pollutant removal agent, which is low in price, natural, non-toxic, easy to obtain, environmentally friendly, efficient and sustainable, simple in method and easy to operate, and suitable for wide application. The present application not only can utilize the breeding waste in a harmless way in a very short period, but also can simultaneously improve the overall reduction efficiency of nearly 500 new biological pollutants, such as 319 main resistance genes, 57 mobile genetic elements and 120 virulence genes in livestock and poultry manure, and synergistically and efficiently reduce the relative abundance and detection number of most resistance genes and virulence genes in livestock and poultry manure. The reduction rate of the functional genes related to the antibiotic resistance and pathogenic bacteria of human and animals is about 60.95-70.31% and 67.85-80.80%.

The present application relates to a kind of plasmid for endowing bacillus to degrade skatole function and its application, belong to agricultural environment microorganism and genetic engineering technical field, to solve the existing skatole management high cost, easy to produce secondary pollution, part of degradation strain exists biological safety risk, and the genetic element of natural degradation strain is difficult to cross-strain application problem.The present application discloses the plasmid of nucleotide sequence as shown in SEQ ID NO.1, by the plasmid is transformed into bacillus competent cell, is cultivated in M9 solid medium containing skatole and positive clone detection obtains recombination bacillus, it is inoculated with 0.5% to 5% inoculation amount in the medium containing 0.05 to 0.2g / L skatole, under the condition of 30 to 37 DEG C, 120 to 250rpm 4 to 48h can be degraded skatole.The present application is mainly used for the skatole deodorization of breeding manure and the like scene, provides safe controllable, efficient and scalable biological technology scheme.

The present invention discloses a genetic element associated with adipocyte differentiation, namely lncRNA420, with a nucleotide sequence as SEQ ID NO. 1. The present invention first discovered that lncRNA420 sequence expression is associated with bovine preadipocyte differentiation. As the degree of preadipocyte differentiation increases, the expression level of lncRNA420 decreases, and lncRNA420 expression is higher in bovine abdominal fat than in subcutaneous fat. By detecting the expression of lncRNA420, the degree of adipocyte differentiation can be identified. Promoting lncRNA420 expression can inhibit adipocyte differentiation, providing a new method for targeting bovine fat development and lipid metabolism.

A method of transposon insertion sequencing (TIS) capable of resolving insertion sites in or around repeat elements, said method including the steps of; preparing a pool or library of mutant cells by