35 results about "Genetic element" patented technology

Most therapeutic treatments require dose adjustment after initial administration. Dosage adjustment is crucial to ensuring optimal safety and efficacy characteristics of the treatment. Gene therapy is inherently limited by the inability to adjust the dose after initial administration. This invention describes a genetic element capable of dose adjustment after the initial administration of gene therapy. The realization of dose adjustment allows for the replacement of multiple subcutaneous protein injection regimens with single-injection gene therapy, thereby improving pharmacokinetics, safety / efficacy characteristics, medication adherence, and reducing healthcare costs.

This technology provides targeted genome editing techniques for altering the color of parts of grape plants and their fruits. Specifically, this technology relates to using genome editing methods to generate edits in target genes that cause large fragment inactivation deletions, thereby altering the color traits of plants and fruits. These target genes include genes and genetic elements associated with plant color and pigment deposition (e.g., the PDS1 gene, the mybA1 gene, and the Gret1 retrotransposon).

Disclosed are variants of Fas ligands (APTL, FASL, CD178, CD95L, ALPS1B, CD95-L, TNFSF6, TNLG1A, APT1LG1) and vectors providing them. Furthermore, recombinant cells, such as NK cells (natural killer cells), containing genetic elements that enable the production of at least one FasL variant to enhance the cytotoxicity and viability of recombinant cells and improve the therapeutic activity of recombinant cells are disclosed. Also disclosed are pharmaceutical compositions containing recombinant cells and methods for obtaining them, as well as NK cells modified to express Fas ligand variants for use in immunotherapy.

Gene therapy compositions and methods related to transposition are provided, e.g., those engineered from Myotis myotis.

A geminivirus replicon, an in planta directed evolution / selection system based thereon, and use. Provided is a geminivirus-based controllable artificial replicon, which comprises two LIRs derived from BeYDV; the two LIRs are located at two flanks of the artificial replicon, respectively. Also provided is an in planta directed evolution / selection system, which comprises the geminivirus-based controllable artificial replicon, a Rep and / or RepA protein, and a mutant or mutant library of a genetic element. The replication of the geminivirus-based controllable artificial replicon is configured to be associated with the desired function of the mutant of the genetic element. The provided in planta directed evolution / selection system is a universal, rapid, and efficient in planta directed evolution system, and can be used for generating new excellent alleles and assisting future agricultural breeding.

The invention relates to a microbial strain DNA (deoxyribonucleic acid) mixed coding and an alternating Transformer depth prediction method for gene expression of the microbial strain DNA mixed coding, and belongs to the crossing field of bioinformatics and artificial intelligence. The core of the method is to construct a deep learning model capable of efficiently capturing DNA sequence characteristics, and end-to-end accurate prediction of the expression level of a target gene in a specific microbial host is realized. The method comprises the following steps: carrying out binary coding on four conventional basic groups of DNA of a target strain, splicing eight key biological characteristics closely related to gene expression, and carrying out hybrid coding to generate a combined characteristic matrix; a deep learning network containing an alternating Transform coding structure is established, local structure information of a DNA combination feature matrix and a global context dependency relationship are deeply fused, and the analysis precision of microorganism DNA sequence data and the prediction accuracy of a gene expression level are improved. According to the technical scheme, an efficient and accurate computer-aided prediction tool is provided for optimization design of genetic elements (such as promoters) and gene sequences, the expensive experiment trial and error cost and the strain characterization cost can be remarkably reduced, and the research and development process of high-yield strains is accelerated.

The present invention relates generally to methods and materials for boosting gene expression in eukaryotic cells, for example the expression of heterologous genes encoding proteins of interest. In particular, it relates to gene expression cassettes with increased expression rates.

Provided are cells that are resistant to mobile genetic elements or horizontal gene transfer, and methods for obtaining said cells. Also provided are methods for preventing the horizontal transfer of genetic information between a mobile genetic element and a first cell, cells making use of new genetic codons schemes and related subject matter, kits comprising mutually orthogonal cells, and mobile genetic elements. Also provided are methods of altering the susceptibility of a gene to mutations that alter the encoded amino acid sequence, methods for evolving or improving a protein, and methods for rendering a target gene more resistant to mutation. Additionally provided are uses of the cells for making polymers and methods comprising using the cells for making polymers.

Here, the inventors use genomics to discover a new family of lanthipeptides from Actinobacteria dedicated to anti-phage defense. They demonstrate that a specific family of metabolic pathways that produce unknown class I lanthipeptides are encoded near known anti-phage defense systems or within mobile genetic elements, indicating their anti-phage function. Furthermore, the inventors show that the heterologous expression of these pathways in Streptomyces albus (a model strain of Actinobacteria) confers anti-phage defense. The experimental results show that different lanthipeptides of this family confer a selective protection against phages, without compromising the cell viability. The inventors also have identified a specific regulatory system that controls the expression of "defensive lanthipeptides" in Actinobacteria and use this to demonstrate their anti-phage function in a native strain. Finally, the inventors explore the anti-phage mechanism of action of these compounds. Thus the present invention relates to a new class of anti-phage natural peptides.

The invention provides a method for identifying, quantifying and visualizing genomes of drug-resistant pathogenic bacteria in environmental samples, which is suitable for identifying dangerous drug-resistant pathogenic bacteria in various environments. The method comprises the following steps: (1) based on a metagenome sequencing big data set, obtaining a high-quality bacterial genome by using genome quality control, assembly and binning technologies; (2) identifying a drug-resistant bacterium genome carrying an antibiotic drug-resistant gene, further annotating a virulence factor, and comparing with an authoritative pathogenic bacterium directory to realize accurate identification of drug-resistant pathogenic bacteria; and (3) mapping the reference genome of the drug-resistant pathogenic bacteria through the metagenome so as to quantify the abundance of the drug-resistant pathogenic bacteria, and further carrying out visual analysis on the drug-resistant pathogenic bacteria mediated drug-resistant genes, related movable genetic elements and other gene neighborhood structures. The method does not depend on isolated culture, realizes full-spectrum analysis of drug-resistant pathogenic bacteria in environmental samples, and provides important technical support for accurate and scientific evaluation of drug resistance risks of environmental bacteria antibiotics.

The application belongs to the technical field of biomedicine, and provides an alginate-based thermal genetics CXCL12 releaser, a preparation method and application thereof. The application uses transgenic and freeze-drying technology to prepare an alginate-based thermal genetics CXCL12 releaser (ATCG) based on an optimized alternating magnetic field. The preparation is convenient and simple, and can be produced on a large scale. The ATCG is composed of a porous magnetic support loaded with engineering cells. The engineering cells are stably transfected with a pHSP70-CXCL12 thermal genetics element by a lentivirus. The porous magnetic support is composed of a main frame structure of a biopolymer material wrapped with magnetic particles, and has an isotropic porous structure. The ATCG prepared by the application can controllably release CXCL12 chemotactic factors for DTC recruitment, and can activate the anti-DTC peritoneal immunity after magnetic heat ablation, thereby inhibiting the peritoneal metastasis of DTC.

Described herein are methods for modulating the expression of complex gene networks by increasing expression of an RNA-encoding therapeutic genetic element that is normally attenuated in response to an internal or environmental signal while simultaneously disrupting expression of an endogenous RNA-encoding sequence that is normally activated in response to an internal or environmental signal. Methods for enhancing cell therapies and treatment of conditions related to aberrant gene expression are also described.

Vectors, cell lines comprising the vectors, recombinant virions produced from the vectors, and methods of using the vectors for barcoding nuclei of cells are provided. Vector libraries that collectively encode a plurality of genetic elements of interest for screening and methods of screening cells having nuclei barcoded according to the subject methods are also provided.

The invention discloses an editor fusing 5 'exonuclease and CRISPR-Cas12i2 as well as construction and application of the editor, and relates to the technical field of plant biology. The editor for fusing the 5'exonuclease and the CRISPR-Cas12i2 comprises a 5 'exonuclease T5E and Cas12i2 variant fusion protein T5E-Cas12i2 v1 coded by a nucleotide sequence, and a nuclear localization signal bpNLS, the 5' exonuclease T5E, the Cas12i2 variant Cas12i2 v1 and a nuclear localization signal bpNLS are sequentially arranged from the N end to the C end. Compared with an existing plant genome editing tool, the constructed T5E-Cas12i2v1 editor has the advantages that the efficiency is high, TTN-PAM sites are targeted, and site-specific large fragment deletion can be generated. The method is beneficial to accurate manipulation research on large cis-genetic elements, and has a wide application prospect in plant functional genome research and genetic improvement of crops.

This invention relates to GM002714 The application of genes, recombinant vectors containing these genes, and recombinant bacteria in increasing the yield of Streptomyces natural products belongs to the field of genetic engineering technology. To discover and utilize yield-related genetic elements in the Streptomyces metabolic network to improve the yield of Streptomyces natural products, this invention utilizes transposon mutagenesis technology to discover and elucidate for the first time in Streptomyces the function of a two-component regulatory system involved in citric acid metabolism—TCS2713. By constructing a recombinant vector and recombinant bacteria containing the gene encoding the transcriptional regulator GM002714 of this two-component regulatory system, overexpression was observed in *Streptomyces avermitilis*, *Streptomyces cerevisiae*, *Streptomyces glabripennis*, or *Streptomyces roseospora*. GM002714 Genes can increase the yield of natural products. This invention provides valuable biosynthetic elements for the efficient biomanufacturing of Streptomyces.

This invention belongs to the field of solid waste resource utilization technology, and relates to a method for synergistic reduction of antibiotic resistance genes in anaerobic digester residues through hydrothermal-induced iron-calcium-magnesium-silicon-aluminum mineralization. Anaerobic digester residues are used as the treatment target. After screening, homogenization, and solids content adjustment, a composite mineralization inducer is added and the pH is adjusted, and a mineralization reaction is carried out under closed hydrothermal conditions. During the reaction, the hydrothermal effect promotes the rupture of resistant bacterial cells, the deconstruction of extracellular polymers, and DNA strand breaks in the digester residues. Simultaneously, iron, calcium, magnesium, silicon, and aluminum components undergo in-situ mineralization with phosphates, carbonates, humic substances, and dissolved organic matter in the digester residues, forming a composite mineral phase. This complexes, precipitates, encapsulates, and passivates antibiotic resistance gene fragments, mobile genetic elements, extracellular DNA, antibiotic residues, and heavy metals. This method achieves synergistic reduction of resistance genes, decrease in migration risk, weakening of selection pressure, and resource utilization of digester residues, and has advantages such as short treatment cycle, high reduction efficiency, good product stability, and strong engineering applicability.

The application discloses a genetic element for enhancing lactic acid metabolic flux of actinomycetes, and belongs to the field of synthetic biology and microbial metabolic engineering. sucC The application successfully constructs a single expression element and a co-expression element by cloning genes from Streptomyces coelicolor sucD , and verifies that the SucC protein and the SucD protein both have lactic acid coenzyme A synthetase activity through in-vitro protein purification and enzyme activity determination, and the enzyme activity of a protein complex (SucC-SucD, SucCD) expressed by the co-expression element is significantly higher than that of the single protein, indicating that the two proteins have a synergistic effect; the genetic element can efficiently catalyze the combination reaction of lactic acid and coenzyme A in vitro, accelerates the lactic acid metabolic speed, provides a core genetic element for the modification of the lactic acid metabolic pathway of actinomycetes, enriches a synthetic biology tool library, and has important industrial application prospects and academic value.

The present application belongs to the technical field of excrement pollution prevention and treatment, and relates to a pollutant removal promoter and application thereof. The present application uses fresh earthworm body fluid and earthworm manure mixture as raw material of the pollutant removal agent, which is low in price, natural, non-toxic, easy to obtain, environmentally friendly, efficient and sustainable, simple in method and easy to operate, and suitable for wide application. The present application not only can utilize the breeding waste in a harmless way in a very short period, but also can simultaneously improve the overall reduction efficiency of nearly 500 new biological pollutants, such as 319 main resistance genes, 57 mobile genetic elements and 120 virulence genes in livestock and poultry manure, and synergistically and efficiently reduce the relative abundance and detection number of most resistance genes and virulence genes in livestock and poultry manure. The reduction rate of the functional genes related to the antibiotic resistance and pathogenic bacteria of human and animals is about 60.95-70.31% and 67.85-80.80%.

The present application relates to a kind of plasmid for endowing bacillus to degrade skatole function and its application, belong to agricultural environment microorganism and genetic engineering technical field, to solve the existing skatole management high cost, easy to produce secondary pollution, part of degradation strain exists biological safety risk, and the genetic element of natural degradation strain is difficult to cross-strain application problem.The present application discloses the plasmid of nucleotide sequence as shown in SEQ ID NO.1, by the plasmid is transformed into bacillus competent cell, is cultivated in M9 solid medium containing skatole and positive clone detection obtains recombination bacillus, it is inoculated with 0.5% to 5% inoculation amount in the medium containing 0.05 to 0.2g / L skatole, under the condition of 30 to 37 DEG C, 120 to 250rpm 4 to 48h can be degraded skatole.The present application is mainly used for the skatole deodorization of breeding manure and the like scene, provides safe controllable, efficient and scalable biological technology scheme.

A method of transposon insertion sequencing (TIS) capable of resolving insertion sites in or around repeat elements, said method including the steps of; preparing a pool or library of mutant cells by

A directed evolution method based on geminiviruses is provided, which is a directed evolution method using in vivo screening of genetic elements by primary and secondary replicons of geminiviruses in plant cells.

The application discloses a genetic element for accelerating morphological differentiation of actinomycetes, and belongs to the fields of synthetic biology and molecular biology. eshA Gene overexpression vector pIB139-PermE-his- eshA -his is transformed into target actinomycetes streptomyces coelicolor, and an engineering strain is obtained through screening, and through plate phenotype observation and growth curve determination, the significant effect of gene overexpression is verified. eshA The overexpression strain O eshA S. coelicolor WT appears aerial hyphae and mature spores earlier. The application provides a simple and specific technical scheme for controlling the speed of morphological differentiation of actinomycetes, provides a new genetic element for accelerating morphological differentiation of actinomycetes, and has important application value.​

Systems and methods for mammalian cell genetic manipulation are described herein. Non-human animal models utilizing the systems, methods of generating the non-human animals, and methods of using the non-human animals are also described.

The invention relates to the technical field of genetic engineering and bioinformatics, in particular to a method for creating a new gene in a living body on the premise of no artificial DNA template and application. The method is characterized in that DNA fractures are simultaneously generated at at least two different specific positions in the genome of an organism, the specific positions are genomic sites capable of segmenting different gene elements or different protein structural domains, and the DNA fractures are connected with one another by means of non-homologous end connection (NHEJ) or homologous repair. And generating a new combination of the different gene elements or different protein structural domains, which is different from the original genome sequence, so as to form a new gene. The new gene provided by the invention can change the growth, development, resistance, yield and other characters of organisms, and has important application value.

Provided herein are methods for assessing ageing process, lifespan, life expectancy, biological age and the rate of aging by determining pangenome gene alterations. Specifically, the methods comprising determination of the alteration of the gene composition in mammals and / or non-living genetic elements (NLGE) associated with mammals and at least one of the following: microbiota and / or NLGE associated with microbiota. The methods can be used for increasing lifespan and / or for inhibiting aging in mammals.

The present invention relates to a novel nucleic acid construct with improved stability and protein expression levels and uses thereof and, more specifically, to a nucleic acid construct comprising: a coding region encoding a polypeptide or a protein; a poly(A) tail having a secondary or tertiary structure; and a non-A sequence at the 3'-terminal region, and to a pharmaceutical composition for vaccine or gene therapy comprising said nucleic acid construct. The nucleic acid construct platform according to the present invention exhibits superior stability and protein expression levels in cells without interference with CDS and UTR sequences, or other genetic elements, and thus may be broadly and advantageously used in fields such as gene therapy and vaccine applications.

This disclosure provides a system for improving productivity and quality of adeno-associated virus (AAV) vectors and virus-like particles produced from packaging cell lines. Different groups of genetic elements required to produce AAV vectors (encoding regions for Rep proteins, Cap proteins, and helper function) are separated into different modules, which are each placed under control of different inducible regulatory elements. Optionally, the cell line is progeny of fused cells, and is thereby optimized for higher levels of virus production. To manufacture the intended AAV vector or particle, packaging cells containing the suite of inducible modules are contacted with (1) a payload vector containing a marker protein or therapeutic cargo, and (2) the inducer molecules for each of the regulatory elements. The viral titer, ratio of full capsids, and functional titer can be optimized by tuning the amount and ratio of each of the inducer molecules used.

This disclosure provides a system for improving productivity and quality of adeno-associated virus (AAV) vectors obtained from hybrid packaging cells. Different groups of genetic elements required to produce AAV vectors (rep, cap, and helper genes) are separated into different modules, which are each placed under control of a different inducible regulatory element. The regulatory elements are designed to be leakproof, to prevent overexpression of genes that would reduce yield. The packaging cells also contain a landing pad to integrate a payload gene. To manufacture the intended AAV vector or particle, the packaging cells are contacted with (1) a pay load vector encoding a therapeutic cargo, and (2) inducer molecules for each of the regulatory elements. Viral production and ratio of full capsids can be optimized by tuning the inducer compounds for each of the modules.

This invention discloses a method for regulating the balance between metabolic load and product synthesis in gluconic acid fermentation, belonging to the field of biomanufacturing and process control technology. The method first integrates a heterologous enzyme gene into *E. coli* to construct a heterologous gluconic acid synthesis pathway; then, it establishes a kinetic mechanism model with key metabolites and heterologous enzyme concentrations as state variables and Miox expression parameters as regulatory inputs; based on the model, it constructs two conflicting optimization objective functions: production loss and enzyme expression burden; it designs a dynamic optimization regulation strategy to predict and optimize Miox expression regulation parameters at discrete decision moments; finally, it converts the parameters into genetic element regulation instructions, combining state monitoring to update the model to form a closed-loop control. This invention can automatically balance product synthesis and metabolic load in a dynamic fermentation environment, improving the stability, yield, and robustness to environmental disturbances in gluconic acid production, providing a solution for metabolic regulation in biomanufacturing.