10 results about "Genetic element" patented technology

Most therapeutic treatments require dose adjustment after initial administration. Dosage adjustment is crucial to ensuring optimal safety and efficacy characteristics of the treatment. Gene therapy is inherently limited by the inability to adjust the dose after initial administration. This invention describes a genetic element capable of dose adjustment after the initial administration of gene therapy. The realization of dose adjustment allows for the replacement of multiple subcutaneous protein injection regimens with single-injection gene therapy, thereby improving pharmacokinetics, safety / efficacy characteristics, medication adherence, and reducing healthcare costs.

This technology provides targeted genome editing techniques for altering the color of parts of grape plants and their fruits. Specifically, this technology relates to using genome editing methods to generate edits in target genes that cause large fragment inactivation deletions, thereby altering the color traits of plants and fruits. These target genes include genes and genetic elements associated with plant color and pigment deposition (e.g., the PDS1 gene, the mybA1 gene, and the Gret1 retrotransposon).

Disclosed are variants of Fas ligands (APTL, FASL, CD178, CD95L, ALPS1B, CD95-L, TNFSF6, TNLG1A, APT1LG1) and vectors providing them. Furthermore, recombinant cells, such as NK cells (natural killer cells), containing genetic elements that enable the production of at least one FasL variant to enhance the cytotoxicity and viability of recombinant cells and improve the therapeutic activity of recombinant cells are disclosed. Also disclosed are pharmaceutical compositions containing recombinant cells and methods for obtaining them, as well as NK cells modified to express Fas ligand variants for use in immunotherapy.

This invention relates to GM002714 The application of genes, recombinant vectors containing these genes, and recombinant bacteria in increasing the yield of Streptomyces natural products belongs to the field of genetic engineering technology. To discover and utilize yield-related genetic elements in the Streptomyces metabolic network to improve the yield of Streptomyces natural products, this invention utilizes transposon mutagenesis technology to discover and elucidate for the first time in Streptomyces the function of a two-component regulatory system involved in citric acid metabolism—TCS2713. By constructing a recombinant vector and recombinant bacteria containing the gene encoding the transcriptional regulator GM002714 of this two-component regulatory system, overexpression was observed in *Streptomyces avermitilis*, *Streptomyces cerevisiae*, *Streptomyces glabripennis*, or *Streptomyces roseospora*. GM002714 Genes can increase the yield of natural products. This invention provides valuable biosynthetic elements for the efficient biomanufacturing of Streptomyces.

This invention belongs to the field of solid waste resource utilization technology, and relates to a method for synergistic reduction of antibiotic resistance genes in anaerobic digester residues through hydrothermal-induced iron-calcium-magnesium-silicon-aluminum mineralization. Anaerobic digester residues are used as the treatment target. After screening, homogenization, and solids content adjustment, a composite mineralization inducer is added and the pH is adjusted, and a mineralization reaction is carried out under closed hydrothermal conditions. During the reaction, the hydrothermal effect promotes the rupture of resistant bacterial cells, the deconstruction of extracellular polymers, and DNA strand breaks in the digester residues. Simultaneously, iron, calcium, magnesium, silicon, and aluminum components undergo in-situ mineralization with phosphates, carbonates, humic substances, and dissolved organic matter in the digester residues, forming a composite mineral phase. This complexes, precipitates, encapsulates, and passivates antibiotic resistance gene fragments, mobile genetic elements, extracellular DNA, antibiotic residues, and heavy metals. This method achieves synergistic reduction of resistance genes, decrease in migration risk, weakening of selection pressure, and resource utilization of digester residues, and has advantages such as short treatment cycle, high reduction efficiency, good product stability, and strong engineering applicability.

The application discloses a genetic element for enhancing lactic acid metabolic flux of actinomycetes, and belongs to the field of synthetic biology and microbial metabolic engineering. sucC The application successfully constructs a single expression element and a co-expression element by cloning genes from Streptomyces coelicolor sucD , and verifies that the SucC protein and the SucD protein both have lactic acid coenzyme A synthetase activity through in-vitro protein purification and enzyme activity determination, and the enzyme activity of a protein complex (SucC-SucD, SucCD) expressed by the co-expression element is significantly higher than that of the single protein, indicating that the two proteins have a synergistic effect; the genetic element can efficiently catalyze the combination reaction of lactic acid and coenzyme A in vitro, accelerates the lactic acid metabolic speed, provides a core genetic element for the modification of the lactic acid metabolic pathway of actinomycetes, enriches a synthetic biology tool library, and has important industrial application prospects and academic value.

The present application relates to a kind of plasmid for endowing bacillus to degrade skatole function and its application, belong to agricultural environment microorganism and genetic engineering technical field, to solve the existing skatole management high cost, easy to produce secondary pollution, part of degradation strain exists biological safety risk, and the genetic element of natural degradation strain is difficult to cross-strain application problem.The present application discloses the plasmid of nucleotide sequence as shown in SEQ ID NO.1, by the plasmid is transformed into bacillus competent cell, is cultivated in M9 solid medium containing skatole and positive clone detection obtains recombination bacillus, it is inoculated with 0.5% to 5% inoculation amount in the medium containing 0.05 to 0.2g / L skatole, under the condition of 30 to 37 DEG C, 120 to 250rpm 4 to 48h can be degraded skatole.The present application is mainly used for the skatole deodorization of breeding manure and the like scene, provides safe controllable, efficient and scalable biological technology scheme.

A method of transposon insertion sequencing (TIS) capable of resolving insertion sites in or around repeat elements, said method including the steps of; preparing a pool or library of mutant cells by

The application discloses a genetic element for accelerating morphological differentiation of actinomycetes, and belongs to the fields of synthetic biology and molecular biology. eshA Gene overexpression vector pIB139-PermE-his- eshA -his is transformed into target actinomycetes streptomyces coelicolor, and an engineering strain is obtained through screening, and through plate phenotype observation and growth curve determination, the significant effect of gene overexpression is verified. eshA The overexpression strain O eshA S. coelicolor WT appears aerial hyphae and mature spores earlier. The application provides a simple and specific technical scheme for controlling the speed of morphological differentiation of actinomycetes, provides a new genetic element for accelerating morphological differentiation of actinomycetes, and has important application value.​

This invention discloses a method for regulating the balance between metabolic load and product synthesis in gluconic acid fermentation, belonging to the field of biomanufacturing and process control technology. The method first integrates a heterologous enzyme gene into *E. coli* to construct a heterologous gluconic acid synthesis pathway; then, it establishes a kinetic mechanism model with key metabolites and heterologous enzyme concentrations as state variables and Miox expression parameters as regulatory inputs; based on the model, it constructs two conflicting optimization objective functions: production loss and enzyme expression burden; it designs a dynamic optimization regulation strategy to predict and optimize Miox expression regulation parameters at discrete decision moments; finally, it converts the parameters into genetic element regulation instructions, combining state monitoring to update the model to form a closed-loop control. This invention can automatically balance product synthesis and metabolic load in a dynamic fermentation environment, improving the stability, yield, and robustness to environmental disturbances in gluconic acid production, providing a solution for metabolic regulation in biomanufacturing.