4 results about "Large fragment" patented technology

The application belongs to the field of molecular biology, and particularly relates to a method for efficiently constructing large fragment deletion of zebrafish helt gene and application. The method for efficiently constructing large fragment deletion of zebrafish helt gene is applied to constructing an animal model for treating and drug screening of Alzheimer disease caused by large fragment deletion of genome. The application also provides a method for efficiently constructing large fragment deletion of zebrafish helt gene. Through design of a target and a primer, a mutant with large fragment deletion of zebrafish helt gene is obtained. The CRISPR / Cas system is used to perform genome large fragment deletion in a zebrafish helt gene cluster. The length of the deleted genome fragment ranges from 1.4 kb, and the average efficiency reaches more than 60%, which greatly improves the efficiency of genome large fragment deletion.

This application discloses a method for preparing adapters and large-fragment sequencing libraries, a sequencing method, a kit for the application, and its applications. The scheme in this application utilizes a specially designed adapter for circularization, enabling simultaneous translation of restriction gaps in two directions. This allows for rapid and accurate capture of information from both ends of large fragments, facilitating genome assembly and variant detection.

The present invention belongs to the field of biotechnology and relates to a multiplex amplification system and kit for 16 Mini Y-STRs, with the amplified fragment length less than 290 bp, which is used to supplement the detection of large fragment loci greater than 350 bp in conventional Y kits on the market, for samples with amplification failure in trace degraded forensic materials. The present invention involves simultaneously amplifying multiple short tandem repeats in a single PCR system. Specifically, the loci are 16 Y short tandem repeats with high genetic polymorphisms. Primers are designed and fluorescent groups are labeled respectively. This system has an extremely high individual recognition rate and non-paternity exclusion rate, a large amount of information, and good compatibility. The present invention fills the current gap in the Y-case market and can be used in combination with all high-locus Y-case kits on the market. When large fragment amplification fails in trace degraded forensic materials, this system can be used to complement the missing large fragments and obtain complete genotyping data of more than 35 loci as much as possible. The present invention has higher accuracy and better sensitivity.