3 results about "Large fragment" patented technology

The application belongs to the field of molecular biology, and particularly relates to a method for efficiently constructing large fragment deletion of zebrafish helt gene and application. The method for efficiently constructing large fragment deletion of zebrafish helt gene is applied to constructing an animal model for treating and drug screening of Alzheimer disease caused by large fragment deletion of genome. The application also provides a method for efficiently constructing large fragment deletion of zebrafish helt gene. Through design of a target and a primer, a mutant with large fragment deletion of zebrafish helt gene is obtained. The CRISPR / Cas system is used to perform genome large fragment deletion in a zebrafish helt gene cluster. The length of the deleted genome fragment ranges from 1.4 kb, and the average efficiency reaches more than 60%, which greatly improves the efficiency of genome large fragment deletion.

This application discloses a method for preparing adapters and large-fragment sequencing libraries, a sequencing method, a kit for the application, and its applications. The scheme in this application utilizes a specially designed adapter for circularization, enabling simultaneous translation of restriction gaps in two directions. This allows for rapid and accurate capture of information from both ends of large fragments, facilitating genome assembly and variant detection.