81 results about "Synthetase activity" patented technology

A polypeptide according to the present invention includes: an altered polypeptide obtained by altering an ArgRS, a CysRS, a MetRS, a GlnRS, a GluRS, a LysRS, a TyrRS, or a TrpRS so that an unnatural amino acid is recognized; and an editing polypeptide derived from a PheRS, a LeuRS, an IleRS, a ValRS, an AlaRS, a ProRS, or a ThrRS, the editing polypeptide having been either inserted between a Rossman-fold N domain and a Rossman-fold C domain that exist in the altered polypeptide, or bound to an N terminal of the altered polypeptide. Thus provided are a new aaRS that exhibits high substrate specificity to an unnatural amino acid and a technique that involves the use of such an aaRS.

The subject invention concerns a method of inhibiting an RNA virus infection within a patient by increasing the amount of 2-5 oligoadenylate synthetase (2-5 AS) activity within the patient. Preferably, the preventative and therapeutic methods of the present invention involve administering a nucleotide encoding 2-5 AS, or at least one catalytically active fragment thereof, such as the p40, p69, p100 subunits, to a patient in need thereof. The present inventors have determined that overexpression of 2-5AS causes a reduction in epithelial cell damage, reduction in infiltration of mononuclear cells in the peribronchiolar and perivascular regions, and reduction in thickening of the septa in the lungs. Levels of chemokines, such as MIP1-alpha, are also reduced upon overexpression of 2-5AS. The subject invention also pertains to pharmaceutical compositions containing a nucleotide sequence encoding 2-5 AS and a pharmaceutically acceptable carrier, as well as vectors for delivery of the 2-5 AS nucleotide sequence.

The invention relates to application of male sterility gene OsLAP5 and a method for recovering rice male sterility, belonging to the technical field of rice breeding. A base sequence of the male sterility gene OsLAP5 is as shown in SEQ ID NO.2. The application is as follows: the OsLAP5 gene is knocked out or inhibited so that activity of rice sporopollenin synthetase loses or expression level of the rice sporopollenin synthetase drops; and an obtained new rice male sterile line can be used for producing hybrid seeds. Furthermore, the invention relates to a method for creating a rice male sterile strain, application of the rice sterile strain in production of rice seeds and a method for recovering a male sterile character. The rice mutant disclosed by the invention has no abnormity in a vegetative growth stage, but a homozygote plant is completely sterile. The method, if applied to hybrid seeding, can avoid an operation of castration of female parent, greatly improve production efficiency and reduce labor cost, and the method has good application on agricultural production.

A mycose synthetase and its use are disclosed. It consists of (a) or (b) protein, (a) protein is made of amino-acid residue sequence in sequence 2; (b) protein is derived by (a) and substituted and/or lost and/or added by one or several amino-acid residue and has mycose synthetase activity. It can be used to produce mycose by catalyzed malt dust.

The present invention features improved methods for producing pantoate and pantothenate utilizing microorganisms having modified pantothenate biosynthetic enzyme activities. In particular, the invention features methods for reducing byproduct formation and increasing yields and purity of desired product. Recombinant microorganisms and conditions for culturing same are also are featured. Also featured are compositions produced by such microorganisms.

The invention discloses an astaxanthin synthetic gene recombinant expression plasmid pET-Ast with a base sequence as shown in SEQ ID NO: 1. The invention also discloses a preparation method of the recombinant expression plasmid pET-Ast, an application of the recombinant plasmid pET-Ast to the detection of astaxanthin synthetase activity of a target receptor, and an application method. After the astaxanthin synthetic gene recombinant expression plasmid pET-Ast provided by the invention is introduced to an Escherichia coli BL21 strain, the accumulation of astaxanthin in a cell body of Escherichia coli is successfully realized, so that an astaxanthin production strain can be accurately and rapidly established. The preparation method of the astaxanthin synthetic gene recombinant expression plasmid pET-Ast is simple and convenient, easy to operate and low in cost. By using the method disclosed by the invention, a gene sequence to be detected in the target receptor can be replaced with a related gene sequence in the recombinant expression plasmid pET-Ast through digestion and connection, and then, the function of a gene to be detected in the target receptor can be simply and effectively detected and verified through detecting whether the astaxanthin is accumulated in a host cell body.

The invention relates to a recombined lipoxygenase PhLOX and a preparation method thereof. The PhLOX has activities of a lipoxygenase, a hydroperoxide lyase and a propadiene oxide synthetase at the same time. The method includes following steps: extracting total RNA from porphyra haitanensis; designing a primer according to a gene sequence of the lipoxygenase; amplifying an overall-length cDNA sequence through an RACE technology; recombining the overall-length cDNA sequence to an expression vector pET-28a; converting escherichia coli E.coli BL21; screening out positive clone; and generating the multifunctional lipoxygenase of a bacteria liquid with an expression quantity being 2mg/L through inducible expression. Compared with a lipoxygenase in the prior art, the recombined lipoxygenase has a plurality of enzyme functions, is free of a strict substrate specifity and allows downstream products in various structures to be formed.

The present invention provides a preventative/therapeutic agent for cancer capable of selectively and effectively killing cancer cells. A medicament of the present invention prepared by combining at least one selected from a substance that inhibits the activity of an enzyme belonging to the acyl-CoA synthase family and a substance that inhibits expression of a gene for an enzyme belonging to the acyl-CoA synthase family, with at least one selected from a substance that inhibits the activity of fatty acid synthase and a substance that inhibits expression of a fatty acid synthase gene and the like can be used as a preventative/therapeutic agent for cancer capable of selectively and effectively killing cancer cells.

The invention discloses healthy tea rice porridge with a function of reducing blood pressure and a preparation method thereof. The tea rice porridge is prepared from 60-70 of millet, 20-30 of corn, 15-25 of green tea, 6-8 of peas, 3-5 of semen coicis, 1-3 of white sesame, 1-3 of kudzuvine root, 1-2 of fructus citri sarcodactylis, 0.8-1.2 of Japanese thistle root, 0.8-1.2 of immature bitter orange, 0.5-1 of pawpaw, 0.5-1 of pumpkin, 1-1.5 of Chinese dwarf cherry leaves, 1-1.5 of grosvenor momordica fruit leaves and white granulated sugar. The rice porridge produced by the preparation method has the advantages that the rice porridge has the special fragrance of tea and is tasty; the added kudzuvine root can inhibit excessive release of ET, promote endothelium recovery to excite PG12 synthetase activity, obviously increase EC to generate PG and inhibit platelet aggregation, and has the functions of improving blood circulation and reducing the blood pressure; the Japanese thistle root can reinforce myocardial contractility, improve heart functions, increase myocardial blood flow, dilate peripheral vessels, increase blood flow and reduce the blood pressure; and the product also contains various traditional Chinese medicine ingredients beneficial to human bodies, is suitable for people of all ages and is beneficial to body health after being eaten for a long time.

The invention discloses a pesticide composition containing pyriproxyfen and hexaflumuron, and belongs to the technical field of pesticides. Pyriproxyfen and hexaflumuron in the pesticide composition belong to insect growth regulators; and functional mechanisms of pyriproxyfen and hexaflumuron are different, and pyriproxyfen belongs to juvenile hormone analogues, which disturbs the insect hormone balance, while hexaflumuron belongs to chitin synthesis inhibitor class, which has functions of inhibiting the chitin synthetase activity and hindering a larval molting effect. According to the pesticide composition, pyriproxyfen and hexaflumuron are mixed to play an obvious synergistic interaction function on leek late eye fungus gnat 4-instar larva, thereby improving the prevention and control effect, greatly reducing the dosage in the field, effectively reducing environmental pollution and pesticide residues and reducing the cost. In addition, two single dosages have different mechanical functions, so that the overcoming of injurious insect resistance and the delaying of the injurious insect resistance are facilitated due to the mixed using of the two single dosages. The pesticide composition containing pyriproxyfen and hexaflumuron is low in toxicity, quick in response and wide in disease prevention and treatment, and has a good pesticide effect.

The invention discloses a Tulipa fosteriana flavonol synthase TfFLS protein and a coding gene thereof. The protein is a protein composed by an amino acid sequence shown in SEQ ID No. 2 or a protein composed of an amino acid sequence obtained through replacement, deletion or addition of one or more amino acids of the amino acid sequence shown in the SEQ ID No. 2 and having the activity of Tulipa fosteriana flavonol synthase. The invention further provides a nucleic acid sequence used for coding the protein, and the nucleic acid sequence is shown in SEQ ID No. 1. The Tulipa fosteriana flavonol synthase TfFLS gene provided by the invention expresses a recombinant flavonol synthase protein in Escherichia coli cells and enables dihydrokaempferol to produce kaempferol, which proves that the gene has the functions of flavonol synthase. With the TfFLS gene, substances, receptors, inhibitors, antagonists or the like interacting with TfFLS can be screened by using a variety of conventional screening methods.

The present invention relates to an isolated DNA which comprises a DNA sequence encoding avermectin aglycon synthase domains that corresponds to multifunctional enzyme proteins involved in the biosynthesis of a polyketide compound, or its mutants having avermectin aglycon synthase activity, particularly functional modules and submodules in the DNA sequence derived from Streptomyces avermitilis, a polypeptide or mutants thereof encoded by the DNA or the mutants, a vector containing the DNA or the mutants, a host cell transformed with the DNA, the mutants thereof, or the vector, and a method for producing avermectin.

Recombinant E. coli having enhanced acetyl-CoA synthetase activity and the ability to produce glycerol and glycerol-derived products, such as 3-hydroxypropionic acid, methylglyoxal, 1,2-propanediol, and 1,3-propanediol, are described. The recombinant E. coli comprise a promoter operably linked to a nucleotide sequence encoding a polypeptide having acetyl-CoA synthetase enzyme activity, wherein the promoter and nucleotide sequence are each independently either native or non-native.

The present invention relates to an enzyme determining amino acid sequences of an enzyme involved in pyrethrin biosynthesis and a base sequence of the gene thereof; constructing vectors bearing the gene and transformants; and extractable from plant bodies producing pyrethrin by applying such creative techniques to plant bodies with faster growth aiming to provide a method to efficiently produce pyrethrin; and the enzyme is a gene encoding a protein of the following (i) or (ii):

• • (i) a protein consisting of an amino acid sequence shown in Sequence No. 1; or
  • (ii) a protein consisting of an amino acid sequence including one or more of a substitution, deletion, insertion, and/or addition of amino acid in the amino acid sequence shown in Sequence No. 1, in which the protein exhibits activity of pyrethrin biosynthetic enzyme.

A series of independent non-transgenic mutations found in at least one deoxyhypusine synthase gene of tomato; tomato plants having these mutations in one or more of their deoxyhypusine synthase genes; and a method of creating and identifying similar and/or additional mutations in a deoxyhypusine synthase gene by screening pooled and/or individual tomato plants. The tomato plants of the present invention exhibit altered deoxyhypusine synthase activity and delayed post harvest softening of their tomato fruit without having the inclusion of foreign nucleic acids in their genomes. Novel nucleotide and protein sequences for deoxyhypusine synthases in tomato and their uses.

The present invention relates to protein fragments of 5-enolpyruvylshikimate-3-phosphate synthase, which are selected from the protein fragment pairs of EPSPS, two such protein fragments can make up full length EPSPS and reconstitute EPSPS activities by complementation without help of any joint structure. The present invention also relates to nucleic acid molecules encoding the protein fragments, expression vectors and cells comprising such nucleic acid molecules. The present invention also relates to methods for reconstituting EPSPS activities by using the fragments or the nucleic acid molecules or the expression vectors of the present invention, as well as methods for dividing the protein fragments of the present invention.

A DNA fragment which encodes a polypeptide defined in the following (a) or (b), and a polypeptide defined in the following (c) or (d):

• [0001]
  • (a) a polypeptide which has at least the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID NO: 2 shown in Sequence Listing,
  [0002]
  • (b) a polypeptide which has at least the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID No: 2 shown in Sequence Listing including substitution, deletion, insertion, addition, or inversion of one or several amino acids, and can constitute a protein having a carbamoyl-phosphate synthetase activity with a large subunit of carbamoyl-phosphate synthetase having the amino acid sequence comprising at least the amino acid numbers 55 to 1113 of SEQ ID NO: 3,
  [0003]
  • (c) a polypeptide which has the amino acid sequence comprising at least the amino acid numbers 55 to 1113 of SEQ ID NO: 3 shown in Sequence Listing,
  [0004]
  • (d) a polypeptide which has the amino acid sequence comprising at least the amino acid numbers 55 to 1113 of SEQ ID NO: 3 shown in Sequence Listing including substitution, deletion, insertion, addition, or inversion of one or several amino acids, and can constitute a protein having a carbamoyl-phosphate synthetase activity with a small subunit of carbamoyl-phosphate synthetase having the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID NO: 2.

A method of performing high-throughput screening of various enzymatic activities with high sensitivity using artificial genetic circuits is provided. Particularly, the invention is screening and quantifying the activity of isoprene biosynthesis enzymes using an artificial genetic circuit capable of sensing isoprene. The artificial genetic circuit comprises an isoprene-sensing transcriptional regulator which recognizes isoprene, at least one reporter gene, a isoprene-sensing transcriptional regulator binding region and promoters for genes encoding isoprene-sensing transcriptional regulators and reporter proteins.

The artificial genetic circuit detects isoprene liberated from many enzymatic reactions and measures the activity of reporter genes. This system is widely applicable for high throughput and quantitative screening of isoprene biosynthesis enzymes and MEP/MVA pathway enzymes.

Therefore, the invention can be advantageously used in the protein engineering technology for enzyme modification. Particularly, it can provide a quantitative investigation of enzymatic activity, and thus can be applied to molecular evolution technology.