3162 results about "Base sequence" patented technology

The present invention provides a polynucleotide that not only has a high RNA interference effect on its target gene, but also has a very small risk of causing RNA interference against a gene unrelated to the target gene. A sequence segment conforming to the following rules (a) to (d) is searched from the base sequences of a target gene for RNA interference and, based on the search results, a polynucleotide capable of causing RNAi is designed, synthesized, etc.:(a) The 3′ end base is adenine, thymine, or uracil,(b) The 5′ end base is guanine or cytosine,(c) A 7-base sequence from the 3′ end is rich in one or more types of bases selected from the group consisting of adenine, thymine, and uracil, and(d) The number of bases is within a range that allows RNA interference to occur without causing cytotoxicity.

The surface of a semiconductor substrate (1) comprises an input diode section (2) and a floating diffusion section (3) consisting of a diffusion region reverse to the substrate in conductivity type, an input gate (6) and an output gate (7) fixed on an insulation film (5) extending from an input diode section to a floating diffusion section, a sensing section (9) consisting of an ion sensitive film fixed on the insulation film extending from the input.

Transmission with multiple antennas in a wireless network is performed by transmitting a plurality of reference sequences (RS) from a UE. A first RS s1[k] is produced using a first cyclic shift and a base sequence s0[k], wherein k={1, 2 . . . K} is an element index. A second RS s2[k] is produced using a second cyclic shift and s0[k]. A first symbol sequence x1[k] is produced using at least s1[k] and s2[k], for at least one k. A second symbol sequence x2[k] is produced using at least s1[k] and s2[k], for at least one k. x1[k] is transmitted using a first transmit antenna and x2[k] is transmitted using a second transmit antenna.

Since conventional DNA sequence analyzing technologies are based on the fundamental principle of fluorescent detection, expensive, complex optical systems and laser sources have been necessary.A field-effect device for gene detection of the present invention analyzes a base sequence by immobilizing a single-strand nucleic acid probe at a gate portion, inducing hybridization at the gate portion to form a double-stranded DNA, inducing elongation reaction by adding a DNA polymerase and one of the substrates, and measuring the electrical characteristic of the field-effect device caused by elongation reaction.Since the elongation reaction of one base induced at the gate portion can be directly converted to an electrical signal, expensive lasers or complex optical systems are not needed. Thus, a small gene polymorphism detection system that can conduct measurement at high precision can be provided.

Convenient techniques for discriminating the base type in a base sequence of a nucleic acid are provided. The technique includes the step (a) of preparing a sample solution containing a nucleic acid, a primer having a base sequence that includes a complementary binding region which complementarily binds to the nucleic acid, and a nucleotide; the step (b) of allowing the sample solution to stand under a condition to cause an extension reaction of the primer, and producing pyrophosphate when the extension reaction is caused; the step (c) of bringing the sample solution into contact with the front face of a H+ hardly permeable membrane having H+-pyrophosphatase, which penetrates from front to back of the membrane, of which active site that hydrolyzes pyrophosphate being exposed to the front face; the step (d) of measuring the H+ concentration of at least either one of the solution at the front face side of the H+ hardly permeable membrane or the solution at the back face side of the H+ hardly permeable membrane, in a state where the H+-pyrophosphatase is immersed in the solution; the step (e) of detecting the extension reaction on the basis of the result of measurement in the step (d) ; and the step (f) of discriminating the base type in the base sequence of the nucleic acid on the basis of the result of detection in the step (e).

The present invention relates to a process for amplifying DNA of an organism. More particularly, the present invention is directed to a process for amplifying DNA of an organism through Polymerase Chain Reaction(PCR) without any information regarding a primer needed for amplifying DNA of an organism.

The surface of a semiconductor substrate (1) comprises an input diode section (2) and a floating diffusion section (3) consisting of a diffusion region reverse to the substrate in conductivity type, an input gate (6) and an output gate (7) fixed on an insulation film (5) extending from an input diode section to a floating diffusion section, a sensing section (9) consisting of an ion sensitive film fixed on the insulation film extending from the input.

The invention relates to a method for producing polymers, in particular synthetic nucleic acid double strands of optional sequence, comprising the steps:(a) provision of a support having a surface area which contains a plurality of individual reaction areas,(b) location-resolved synthesis of nucleic acid fragments having in each case different base sequences in several of the individual reaction areas, and(c) detachment of the nucleic acid fragments from individual reaction areas.

A method of transmitting a control signal in a wireless communication system is provided. The method includes acquiring a resource index, the number of cyclic shifts (CSs) and a CS interval, wherein the number of CSs is an integer multiple of the CS interval, determining a CS index based on the number of CSs and the CS interval, generating a cyclically shifted sequence by cyclically shifting a base sequence by a CS amount obtained from the CS index, generating a modulated sequence based on the cyclically shifted sequence and a symbol for a control signal and transmitting the modulated sequence after mapping the modulated sequence to a resource block obtained from the resource index.

A method and composition for treating colorectal cancer is disclosed. The method involves administering to a subject, a therapeutically effective amount of a morpholino antisense compound (i) having a nuclease-resistant backbone, (ii) capable of uptake by target cancer cells in the subject, (iii) containing between 10-40 nucleotide bases, and (iv) having a base sequence effective to hybridize to a region of processed or preprocessed human SNAIL RNA transcript.

Since conventional DNA sequence analyzing technologies are based on the fundamental principle of fluorescent detection, expensive, complex optical systems and laser sources have been necessary.A field-effect device for gene detection of the present invention analyzes a base sequence by immobilizing a single-strand nucleic acid probe at a gate portion, inducing hybridization at the gate portion to form a double-stranded DNA, inducing elongation reaction by adding a DNA polymerase and one of the substrates, and measuring the electrical characteristic of the field-effect device caused by elongation reaction.Since the elongation reaction of one base induced at the gate portion can be directly converted to an electrical signal, expensive lasers or complex optical systems are not needed. Thus, a small gene polymorphism detection system that can conduct measurement at high precision can be provided.

Methods and apparatus for remapping and regrouping transmission resources in a wireless communication system. First, a set of new permutation algorithms based on Galois field operation is proposed. Then the proposed algorithms and the known Pruned Bit Reversal Ordering (PBRO) algorithm are applied to several of various resource mapping schemes, including slot or symbol level Orthogonal Cover (OC) / Cyclic Shift (CS) mapping, cell-specific slot-level and symbol-level CS hopping patterns, and subframe and slot level base sequence hopping patterns.

An array of hybridization probes, each of which comprises a mass label linked to a known base sequence of predetermined length, wherein each mass label of the array, optionally together with the known base sequence, is relatable to that base sequence by mass spectrometry.

A method, an apparatus and a computer program product are described for automatically generating a state-based program for a component of a system consisting of a plurality of components communicating with each other, wherein the program is generated from a specification of the system, the specification comprising interaction-based sequence descriptions of the system. According to the present invention, all sequence descriptions of said component are determined, the sequence descriptions are normalized, a state-based specification of said component is determined from the normalized sequence descriptions, and the state-based program for the component is determined from the state-based specification. The present invention facilitates the process of program development since the costly, manual development of a state-based program from the specification of a system is automated at least to a substantial degree.

An apparatus and method for a User Equipment in a wireless network including at least one Base Station (BS) are provided. The method includes receiving an Information Element (IE) for configuring at least one of a PUSCH power control method and a PUSCH DMRS generation method from the BS, determining a state of the IE, and transmitting a PUSCH and a DMRS for the PUSCH according to the state of the IE. A transmission power of the PUSCH is controlled and a base sequence for the PUSCH DMRS is generated according to the state of the IE. When the state of the IE indicates a first state, the transmission power of the PUSCH is determined according to a first power control equation. When the state of the IE indicates a second state, the transmission power of the PUSCH is determined according to a second power control equation.

Methods and devices are provided for characterizing a duplex nucleic acid, e.g., a duplex DNA molecule. In the subject methods, a fluid conducting medium that includes a duplex nucleic acid molecule is contacted with a nanopore under the influence of an applied electric field and the resulting changes in current through the nanopore caused by the duplex nucleic acid molecule are monitored. The observed changes in current through the nanopore are then employed as a set of data values to characterize the duplex nucleic acid, where the set of data values may be employed in raw form or manipulated, e.g., into a current blockade profile. Also provided are nanopore devices for practicing the subject methods, where the subject nanopore devices are characterized by the presence of an algorithm which directs a processing means to employ monitored changes in current through a nanopore to characterize a duplex nucleic acid molecule responsible for the current changes. The subject methods and devices find use in a variety of applications, including, among other applications, the identification of an analyte duplex DNA molecule in a sample, the specific base sequence at a single nulceotide polymorphism (SNP), and the sequencing of duplex DNA molecules.

A nucleic acid probe for classification of pathogenic bacterial species is capable of collectively detecting bacterial strains of the same species and differentially detecting them from other bacterial species. Any one of the base sequences of SEQ ID NOS. 90 to 91 and complementary or modified sequences thereof or a combination of at least two of them is used for detecting the gene of an infectious disease pathogenic bacterium.

Various embodiments provide, for example, vectors, expression cassettes, and cells useful for transgenic expression of nucleic acid sequences. In various embodiments, vectors can contain nuclear-based sequences of unicellular photosynthetic bioprocess organisms for the production of food- and feed-stuffs, oils, biofuels, starches, raw materials, pharmaceuticals or fine chemicals.

A probe and a primer capable of collectively detecting microorganisms of the same species while differentiating microorganisms of other species with an object of classification by species of fungus. An oligonucleotide probe for detecting an infectious etiologic microorganism gene includes at least one base sequence selected from the base sequences belonging to one group of the following first to ninth groups. The base sequence groups of first to ninth groups are: first group (SEQ ID NOS:1 to 5); second group (SEQ ID NOS:6 to 10); third group (SEQ ID NOS:11 to 15); fourth group (SEQ ID NOS:16 to 21); fifth group (SEQ ID NOS:22 to 26); sixth group (SEQ ID NOS:27 to 31); seventh group (SEQ ID NOS:32 to 36); eighth group (SEQ ID NOS:37 to 41); and ninth group (SEQ ID NOS:42 to 46).

A nucleic acid probe for classification of pathogenic bacterial species is capable of collectively detecting bacterial strains of the same species and differentially detecting them from other bacterial species. Any one of the base sequences of SEQ ID NOS. 76 to 77 and complementary or modified sequences thereof or a combination of at least two of them is used for detecting the gene of an infectious disease pathogenic bacterium.

A nucleic acid probe for classification of pathogenic bacterial species is capable of collectively detecting bacterial strains of the same species and differentially detecting them from other bacterial species. Any one of the base sequences of SEQ ID NOS. 70 to 72 and complementary or modified sequences thereof or a combination of at least two of them is used for detecting the gene of an infectious disease pathogenic bacterium.

A method for screening presence or absence of a variation in a region of a nucleic acid which comprises the steps of (a) preparing a sample containing a test nucleic acid corresponding to the region, (b) preparing a probe having a base sequence fully complementary to a normal sequence of the region, and a plurality of probes each having at least one base not complementary to the normal sequence, (c) fixing the probes in separate regions on a surface of a substrate to prepare a DNA array substrate, (d) reacting the test nucleic acid with the probes on the DNA array substrate, (e) measuring signals in each region where the signals are originated from respective hybrids formed between the test nucleic acid and one of the probes, and (f) calling variation in the test nucleic acid using a pattern of total signals of all regions.

A router has at least one outbound interface configured for establishing multiple IP-based secure connections (i.e., tunnels) with respective destinations based on transmission of encrypted data packets via the IP-based secure connections. The encrypted data packets are generated by a cryptographic module, where each encrypted packet successively output from the cryptographic module includes a corresponding successively-unique sequence number. The supply of data packets to the cryptographic module is controlled by a queue controller: the queue controller assigns, for each secure connection, a corresponding queuing module configured for outputting a group of data packets associated with the corresponding secure connection according to a corresponding assigned maximum output bandwidth. Each queuing module also is configured for reordering the corresponding group of data packets according to a determined quality of service policy and the corresponding assigned maximum output bandwidth.

A nucleic acid probe for classification of pathogenic bacterial species is capable of collectively detecting bacterial strains of the same species and differentially detecting them from other bacterial species. Any one of the base sequences of SEQ ID NOS. 68 to 69 and complementary or modified sequences thereof or a combination of at least two of them is used for detecting the gene of an infectious disease pathogenic bacterium.

A nucleic acid probe for classification of pathogenic bacterial species is capable of collectively detecting bacterial strains of the same species and differentially detecting them from other bacterial species. Any one of the base sequences of SEQ ID NOS. 87 to 89 and complementary or modified sequences thereof or a combination of at least two of them is used for detecting the gene of an infectious disease pathogenic bacterium.

A router has at least one outbound interface configured for establishing multiple IP-based secure connections (i.e., tunnels) with respective destinations based on transmission of encrypted data packets via the IP-based secure connections. The encrypted data packets are generated by a cryptographic module, where each encrypted packet successively output from the cryptographic module includes a corresponding successively-unique sequence number. The supply of data packets to the cryptographic module is controlled by a queue controller: the queue controller assigns, for each secure connection, a corresponding queuing module configured for outputting a group of data packets associated with the corresponding secure connection according to a corresponding assigned maximum output bandwidth. Each queuing module also is configured for reordering the corresponding group of data packets according to a determined quality of service policy and the corresponding assigned maximum output bandwidth.

A probe, a set of probes, and a probe carrier on which the probe or the set of probes is immobilized, are provided for classification of fungus species. The probe or the set of probes is capable of collectively detecting fungus of the same species and distinguishingly detecting those fungus from fungus of other species. The probe is an oligonucleotide probe for detecting a pathogenic fungus DNA and includes at least one of base sequences of SEQ ID NOS. 1 to 2 and mutated sequences thereof.

A probe, a set of probes, and a probe carrier on which the probe or the set of probes is immobilized, are provided for classification of fungus species. The probe or the set of probes is capable of collectively detecting fungus of the same species and distinguishingly detecting those fungus from fungus of other species. The probe is an oligonucleotide probe for detecting a pathogenic fungus DNA and includes at least one of base sequences of SEQ ID NOS. 1 to 3 and mutated sequences thereof.