595 results about "Base sequence" patented technology

The invention belongs to the technical field of genetic engineering, and particularly relates to recombinant collagen based on engineering bacterium expression and a preparation method thereof, and the recombinant collagen is formed by connecting repetitive units derived from human III type collagen in series for three times; the invention also discloses a base sequence of the coding gene of the recombinant collagen, efficient expression and accurate folding of target protein are realized through genetic modification of engineering bacteria, a light-operated dynamic folding process and an orthogonal purification strategy, and cascade purification of a His6-SUMO tag and an intein-CBD module is utilized to obtain the recombinant collagen. The product purity and the native conformation retention rate are remarkably improved, and the technical problems that hydroxyproline depends on exogenous addition, the folding controllability is poor and the purification efficiency is low in a traditional process are solved.

The present disclosure features useful oligonucleotide compositions and methods related thereto. The present disclosure encompasses the recognition that structural elements of oligonucleotides, such as base sequence, chemical modifications (e.g. modifications of sugar, base and / or internucleotide linkages) or patterns thereof, can have a significant impact on oligonucleotide properties and activities. The present disclosure also provides methods to treat disorders for which deamination of an adenosine in an mRNA produces a therapeutic result, e.g., in a subject in need thereof.

The invention discloses SSR (simple sequence repeat) multiple PCR (polymerase chain reaction) primers for paternity test of grass carp and application of the SSR multiple PCR primers. The primers comprise 16 pairs of specific primers, and base sequences of the 16 pairs of specific primers are respectively shown as SEQ ID NO.1-32 in sequence. The invention further discloses a fish paternity test kit comprising the SSR multiple PCR primer, an SSR multiple fluorescent PCR method for grass carp paternity test and application of the SSR multiple PCR primer, the kit or the method in grass carp paternity test. According to the kit, 16 pairs of specific SSR primers, multiple fluorescent PCR and a universal amplification primer technology are combined, 16 sites can be detected at a time, universal primers can be repeatedly used, and compared with simple single-site detection, the efficiency is improved, and the cost is reduced; the microsatellite loci contained in the invention are 3-6 basic groups, the allele size interpretation is more accurate, and the accuracy of genotype data is improved.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to an SCRE10 gene for improving rice disease resistance and application of the SCRE10 gene, the base sequence of the SCRE10 gene is shown as SEQ ID NO.1, and the amino acid sequence of the SCRE10 gene is shown as SEQ ID NO.2. Through construction of a dexamethasone induced expression SCRE10 transgenic rice plant, it is found that the transgenic rice can significantly induce PR gene expression and active oxygen outbreak, and the resistance of rice to false smut and bacterial leaf blight can be improved. The invention proves that heterologous inducible expression of the Ustilaginoidea virens SCRE10 gene has the function of positively regulating the disease resistance of the rice, and the SCRE10 gene can be used for improving the disease resistance of the rice, which is of great significance to the creation of disease-resistant germplasm of the rice.

The invention relates to a variation identification method of a nanopore single molecule sequencing current signal, which comprises the following steps of: dividing an original current signal generated by nanopore single molecule sequencing into continuous time periods according to a sequence that a basic group passes through a nanopore, calculating an average signal rhythm, constructing a standard time rhythm when a standard basic group sequence passes through the nanopore, and identifying the variation of the nanopore single molecule sequencing current signal. Comparing the offset difference between the actually measured average signal rhythm and the standard time rhythm, wherein a difference salient region is a potential variation region; current signals of front and back neighborhoods are extracted, a current signal disturbance difference value is quantized, and a region with the disturbance difference value having obvious direction asymmetry characteristics is a variation region to be confirmed; the range is expanded, current signals are extracted, disturbance intensity is calculated, and when the disturbance intensity presents a gradually-enhanced or gradually-weakened continuous change trend, it is judged that the variation area to be confirmed has physical rationality; and determining a theoretical current signal by using the corresponding non-variation sequence, comparing the theoretical current signal with an actually measured current signal, calculating a current difference, and determining that the current difference exceeds a normal difference range as a real variation region.

The present invention relates to a method for producing a transgenic plant having an increased saponin content. It is confirmed that a nucleotide having a base sequence represented by SEQ ID NO: 1 or 2 derived from ginseng Panax ginseng increases ginsenoside accumulation by regulating the expression of genes involved in ginsenoside biosynthesis. Therefore, the method can be advantageously applied to the production of transgenic plants with increased saponin content.

The present invention particularly relates to the technical field of data storage, and provides a data encryption method based on DNA encoding, a data decryption method based on DNA encoding, an intelligent terminal, and a medium. A solution comprises: encrypting acquired data to be encoded to obtain encrypted data to be encoded; on the basis of a pre-constructed DNA encoding table, performing DNA encoding on each byte of said encrypted data to obtain an initial base sequence, the DNA encoding table being constructed from four-base units and five-base units; and using a preset DNA sequence structure optimization rule to perform chaotic mapping adjustment and rearrangement on the initial base sequence to obtain an encrypted DNA sequence. According to the solution, the GC content in the encrypted DNA sequence can be kept in a balanced state, and the information density of the encrypted data is increased, such that the encryption effect is effectively improved, thereby reducing a storage space occupied by data to be encoded, improving the security and attack resistance of the data to be encoded, and thus guaranteeing the stability of the encrypted DNA sequence.

The invention discloses a DNA synthesis method which comprises the following steps: processing an initial substrate according to a preset strategy to obtain a target substrate with a Mark pattern and a plurality of reaction sites, and adjusting the positions of the target substrate and an ink-jet printing device through the Mark pattern, so that the working directions of a reaction unit array and the device are consistent, and the subsequent operation accuracy is ensured; deprotecting the reaction sites to generate active groups; sequentially spraying a target monomer solution and an activator solution into the reaction sites of the preset area after deprotection treatment through an ink-jet printing device, and carrying out a coupling reaction with the active groups to obtain a mixed solution containing a nucleotide chain; carrying out cleaning, oxidation, capping and deprotection treatment on the mixed solution to obtain a single-layer base product; according to the target base sequence, determining a corresponding target monomer solution, and circularly carrying out a reaction to obtain a mixed solution containing a nucleotide chain; and obtaining the synthesized DNA according to the mixed solution. According to the method, high-throughput, high-quality and high-efficiency DNA synthesis can be realized.

The invention discloses a CRISPR / Cas12a-RPA (clustered regularly interspaced short palindromic repeats / CRISPR associated protein 12a-recombinase polymerase amplification)-based sequence combination for rapidly detecting mouse hepatitis virus and a kit thereof, and belongs to the technical field of biological detection The sequence combination comprises an RPA primer pair sequence, a crRNA sequence and an ssDNA probe sequence; the kit comprises an RPA primer pair sequence, a crRNA sequence and an ssDNA probe sequence, the kit further comprises a mouse hepatitis virus negative control standard substance, RNase-free water, an RPA reaction buffer solution, an NEB buffer solution, Lba Cas12a nuclease, dry powder RPA reaction microspheres containing recombinase polymerase and magnesium acetate with the concentration of 280 mM. The kit can be used for rapidly detecting the mouse hepatitis virus. The primer sequence combination provided by the invention is good in conservative property and specificity and relatively high in sensitivity, and rapid detection of the mouse hepatitis virus can be realized.

The invention discloses PDRN and a preparation method and application thereof.The base sequence of the PDRN is shown as SEQ ID NO1, and the preparation method of the PDRN comprises the following steps that fermentation treatment is conducted through lactobacillus plantarum, and thalli are collected after an obtained fermentation culture solution is centrifuged; and resuspending the obtained thalli, homogenizing and crushing, centrifuging to remove precipitate, concentrating by using a 5kDa hollow fiber membrane, purifying the obtained concentrated solution by using a Q column, and then concentrating and changing the solution to obtain the high-purity PDRN. According to the method, the PDRN is obtained through fermentation of lactobacillus plantarum for the first time, compared with PDRN sourced from salmon or salmon and the like at present, the method is lower in production cost, and a new way is provided for PDRN production. The PDRN obtained by the method disclosed by the invention is high in purity and single in molecular weight, and experiments prove that the PDRN obtained by the method disclosed by the invention has higher activity in the aspects of promoting cell proliferation, promoting collagen generation and the like.

Disclosed in the present invention is a use of genes NAT1 and bHLH110 in improving high-temperature resistance of rice, for use in using gene editing technology to obtain a rice material having enhanced high-temperature resistance. The base sequence of a rice gene NAT1 is as shown in SEQ ID NO. 1, and the base sequence of a gene bHLH110 is as shown in SEQ ID NO. 4. A base of a rice gene NAT1 of rice is replaced, deleted or inserted through gene editing technology to release inhibition on the rice gene bHLH110, and the expression of wax synthesis genes CER1 and CER1L is promoted by the rice gene bHLH110 to enhance the high-temperature resistance of a plant, thereby preparing a rice material having enhanced high-temperature resistance. According to the present invention, a NAT1 mutant rice material Nipponbare obtained by using CRISPR-Cas9 gene editing technology has remarkably enhanced high-temperature resistance, including an improved survival rate in a seedling stage and improved agronomic traits in a reproductive stage.

A composition of matter includes an antisense phosphorodiamidate morpholino oligomer (MO) that includes an MO base sequence. The MO base sequence is arranged to bind a corresponding complementary base sequence of messenger RNA (mRNA) transcribed from one or more genes for the beta subunit of human chorionic gonadotropin (hCG-β). An inventive method, for suppressing intracellular synthesis of a beta subunit of human chorionic gonadotropin (hCG-β), includes introducing such an MO into one or more cells.

The present disclosure provides double stranded oligonucleotides, compositions, and methods relating thereto. The present disclosure encompasses the recognition that structural elements of double stranded oligonucleotides, such as base sequence, chemical modifications (e.g., modifications of sugar, base, and / or internucleotidic linkages) or patterns thereof, and / or stereochemistry (e.g., stereochemistry of backbone chiral centers (chiral internucleotidic linkages), and / or patterns thereof, can have significant impact on oligonucleotide properties and activities, e.g., RNA interference (RNAi) activity, Ago2 loading, thermal stability, in vivo stability, delivery to tissues and into cells, etc. The present disclosure also provides methods for treatment of diseases, e.g., hepatic diseases, central nervous system (CNS) diseases, etc., using provided double stranded oligonucleotide compositions, for example, in RNA interference.

To detect apple snails from environmental DNA with a high detection rate.SOLUTION: This method amplifies and detects environmental DNA in a sample with a first primer having a specific base sequence, a second primer having another specific base sequence, and a probe having still another base sequence.SELECTED DRAWING: None

The present invention provides a quality control analysis method for third-generation sequencing, which includes performing raw sequencing on the extracted gene sequence to obtain a POD5 text of the initial data. The POD5 text is then converted into a FASTQ file, and adapter data is removed. Quality assessment is then performed to obtain read data. The read data is then aligned and analyzed with the target base sequence of theoretical length, impurities are filtered, and finally, visual detection results are obtained. The present invention also provides a gene extraction method, which forms a highly optimized and reliable bioinformatics analysis process from raw data acquisition, preprocessing, quality assessment, alignment analysis, to final report generation.

The invention relates to the technical field of biology, and discloses an application method of a donkey whole genome 60K liquid phase chip, which comprises the following steps: carrying out nucleic acid extraction on pure biological tissues to obtain biological tissue nucleic acid; the method comprises the following steps: screening biological tissue nucleic acid to obtain target tissue nucleic acid, carrying out fragment connection and mixing on the target tissue nucleic acid to obtain a nucleic acid fragment mixture, carrying out amplification reaction on the nucleic acid fragment mixture to obtain an amplified nucleic acid library, and sequencing the hybridization liquid phase chip to obtain chip sequencing data; performing single-base variation screening on the chip sequencing data to obtain single-base variation data, performing base sequence splicing on the single-base variation data to obtain a single-base sequence, and calculating a genetic effect value corresponding to the donkey sample; and determining a core gene set corresponding to the donkey sample from the chip sequencing data, mining key gene loci in the core gene set, and generating a personalized genetic report of the donkey sample. The method can improve the analysis accuracy of the donkey whole genome.

The invention relates to a group of specific amplification primers for mitochondrial genes of procambarus clarkia and application thereof, and belongs to the technical field of molecular biology, and the mitochondrial genes of the procambarus clarkia comprise cytochrome c oxidase I subunit genes and cytochrome b genes. The specific amplification primer of the CO I gene comprises a CO I gene upstream primer and a CO I gene downstream primer, wherein the base sequences of the CO I gene upstream primer and the CO I gene downstream primer are respectively shown as SEQ NO.1 and SEQ NO.2; the specific amplification primer of the Cytb gene comprises a Cytb gene upstream primer and a Cytb gene downstream primer of which the base sequences are respectively as shown in SEQ NO.3 and SEQ NO.4. The invention further discloses a kit for detecting the Cytb gene. According to the amplification method of the mitochondrial gene of the procambarus clarkia, PCR (Polymerase Chain Reaction) amplification is carried out on a DNA (Deoxyribonucleic Acid) template solution of the procambarus clarkia by adopting amplification primers of a mitochondrial COI gene and a Cytb gene of the procambarus clarkia. The procambarus clarkia mitochondrial gene specific amplification primer has high specificity, a single target gene can be stably amplified, the length of the obtained procambarus clarkia mitochondrial COI gene segment reaches about 1000 bp, and the length of the obtained procambarus clarkia mitochondrial Cytb gene segment reaches about 800 bp.

The invention provides a simplified genome sequencing library construction method and a kit and related application thereof. The construction method of the simplified genome sequencing library comprises the following steps: (1) respectively carrying out enzyme digestion on a plurality of DNA (Deoxyribonucleic Acid) samples by using restriction endonuclease; wherein restriction endonucleases adopted by all the samples are different from one another, and the tail ends of specific DNA fragments of the samples are generated to serve as sample bar codes of the samples; (2) mixing the enzyme digestion products of the samples, constructing a DNA library, carrying out fragment sorting on the DNA library, and taking the selected library as a simplified genome sequencing library; or carrying out fragment sorting on the enzyme digestion product of each sample, and carrying out DNA library construction by using the selected fragments to obtain the simplified genome sequencing library. According to the method, the experimental operation process is simplified, the library building efficiency is improved, and meanwhile, the feasibility of the restriction endonuclease for recognizing 6 basic groups based on a third-generation long-read-long sequencing platform in the simplified genome sequencing technology is also explored.

The invention relates to the field of synthetic biology, in particular to a high-flux biosynthesis ink-jet printing control method and device, and the method comprises the steps: sequentially extracting base data from an obtained DNA base sequence file, and forming a one-dimensional original array; according to a preset printing width, the one-dimensional original array is converted into a two-dimensional array, meanwhile, visualization processing is carried out, corresponding colors are given to different basic groups, and a basic group distribution array diagram with matched row and column structures is obtained; establishing null arrays which correspond to different bases and are consistent in dimension based on the null arrays, traversing the original arrays and assigning values to the corresponding bases at the corresponding positions of the null arrays to obtain a plurality of single base arrays; converting the arrays into a two-dimensional array according to a preset width, and then converting the two-dimensional array into a monochromatic bitmap; and sending the bitmap according to the corresponding relationship between the basic groups and the nozzles and controlling printing. According to the invention, efficient and accurate printing of the base sequence in high-throughput biosynthesis is realized, and the synthesis efficiency and reliability are improved.

The invention discloses a compression and decompression method for DNA sequencing data, and belongs to the technical field of biological information. The technical problems that in the prior art, when third-generation sequencing data are compressed, flexibility is poor, and the compression rate is low are solved. According to the compression method for the DNA sequencing data, global compression or block compression can be dynamically selected before compression, different compression methods are adopted for different types of data, and the flexibility of the compression method is improved; when the base sequence is compressed, the adopted self-indexing structure is a lossless compression method, the initial positions of all the sequences can be compressed and restored by using smaller data volume, and the compression rate is improved. Due to the fact that a special design is adopted in a run length coding structure, a character part is removed, an obtained run length segment is shorter, lossless compression of the position and the sequence is guaranteed, and meanwhile a better compression effect is provided. The method is mainly used for compression and decompression of DNA sequencing data.

The invention relates to the field of optogenetics, in particular to an optogenetics tool for light-operated induced protein degradation and a construction method and application of the optogenetics tool. The invention relates to an optogenetics tool for light-operated induced protein degradation, which is characterized by comprising a fusion system of a truncated body mTRIM211-80 of mTRIM21 and CRY2, the base sequence of the mTRIM211-80 is as shown in SEQ ID NO. 1 (sequence identifier number 1); the base sequence of the CRY2 is as shown in SEQ ID NO. 2. The optogenetics tool is used for inducing the TRIM21 to form a polymer by utilizing the blue light photosensitive protein CRY2 and is used for the targeted regulation and control of the protein degradation under the driving of the blue light. Targeted degradation of protein can be achieved by utilizing Intrabody (intracellular antibody), time and space targeting is achieved, and after an optogenetics toolbox is optimized, the sensitivity and the reaction rate of the optogenetics toolbox are further improved.

The invention relates to the technical field of gene SNP molecular markers, in particular to a gene SNP molecular marker method and system for watermelon breeding, and the method comprises the following steps: respectively extracting DNA solutions of watermelon samples of a plurality of varieties, and performing fingerprint construction based on SNP typing; the method comprises the following steps: acquiring a 16S rRNA sequence of each watermelon flora, and dividing each 16S rRNA sequence into each base sequence; determining mutation associated parameters of each basic group; obtaining a transition probability matrix of each 16S rRNA sequence; correcting the sampling step length of each 16S rRNA sequence; the transition probability matrix is corrected; determining a feature vector of each 16S rRNA sequence; the genetic distance between the watermelon flora and the 16S rRNA sequence of the soil sample is determined, a phylogenetic tree is constructed, and the strongly correlated flora of the breeding environment of each variety of watermelon samples is determined. Therefore, the suitability of the watermelon breeding environment is improved.

The invention belongs to the technical field of biological medicine. The invention relates to a signal peptide, in particular to a signal peptide of GLP-1 and an analogue thereof and application thereof. The invention provides a signal peptide of a GLP-1 protein or an analogue thereof. The amino acid sequence of the signal peptide is shown as any one of SEQ ID NO.1-8. The signal peptide of the GLP-1 protein is improved, so that the signal peptide can promote the generation of an inclusion body and optimize the property of the inclusion body, and also can improve the unit yield of the target protein, and finally, the target protein can obtain more efficient expression. Besides, while the signal peptide is truncated, the base sequence of the target protein is optimized, the character of the inclusion body is changed, and the character of the inclusion body is further optimized. When the fusion protein is used for preparing the GLP-1 protein analogue, the proportion of the target protein in an inclusion body can be greatly improved while the original high inclusion body yield is ensured, the purpose of improving the productivity from the source is finally achieved, and the fusion protein has good application prospects and value.

The invention discloses research of Pdh1 allelic variation in soybean breeding material distribution and pod cracking resistance, and pod cracking severely affects improvement of soybean productivity and is one of important indexes of soybean breeding selection. The invention discloses a high-quality anti-pod-cracking soybean breeding identification marker Pdh1-2 gene, the Pdh1-2 gene is a variation type of a Pdh1 gene, the base sequence of the Pdh1 gene is as shown in SEQ ID NO.2, and the base sequence of the Pdh1-2 gene is as shown in SEQ ID NO.3. The invention also discloses a preparation method of the high-quality anti-pod-cracking soybean breeding identification marker Pdh1-2 gene. The practicability of the Pdh1-2 variation type is further explored. 1118 breeding strains are randomly selected, the proportions of split pods and non-split pods of variation types are respectively counted, and the result shows that 975 parts of materials are Pdh1-1 and Pdh1-3 variation types, and 143 parts of materials are Pdh1-2 variation types. The combined phenotypic analysis shows that the proportion of the Pdh1-2 split pod material is the maximum and is 84.6%, the proportion of the Pdh1-1 is 81.0%, and the proportion of the Pdh1-3 is the minimum and is only 41.1%. The research provides a theoretical basis for breeding of southern anti-pod-cracking soybeans assisted by the molecular marker.

The invention is suitable for the technical field of gene sequencing, and provides a nanopore sequencing signal identification method and system based on Transform, and the method comprises the following steps: carrying out normalization processing on a nanopore sequencing signal, and segmenting the nanopore sequencing signal into signal windows with fixed lengths; signal features are extracted through a Transformer encoder comprising a convolutional layer and a multi-head self-attention module; generating a prediction probability of a current base through a cross attention mechanism by adopting an autoregressive Transform decoder and combining the signal features and context information of a decoded base sequence; iteratively generating an optimal base sequence based on a beam search algorithm, and optimizing decoding efficiency in combination with key value cache; and carrying out overlapping region fusion and quality control on a segmented decoding result, and outputting a final sequence. According to the method, the precision and reliability of nanopore sequencing in complex biological sequence analysis are remarkably improved, and the recognition accuracy on multiple gene sequencing data sets is higher than that of an existing CTC-based model.

The invention discloses SSR (Simple Sequence Repeat) multiple PCR (Polymerase Chain Reaction) primers for megalobrama amblycephala paternity test and application, the primers comprise seven pairs of specific primers, namely a primer pair MamGLB2501, a primer pair MamGLB2502, a primer pair MamGLB2503, a primer pair MamGLG2504, a primer pair MamGLG2505, a primer pair MamGLR2506 and a primer pair MamGLR2507, and the base sequences of the seven pairs of specific primers are sequentially shown as SEQ ID NO.1-14; the invention also discloses an SSR multiplex fluorescent PCR method for megalobrama amblycephala paternity test by using the primer and application of the primer or the method in megalobrama amblycephala paternity test. The microsatellite site amplified by the primer is 3-6 base repetition, the typing is accurate, and the polymorphism of the amplified site is high; the method is accurate in identification and low in price.

The invention discloses a recombinant CapA protein of cat astrovirus. The recombinant CapA protein has an amino acid sequence as shown in SEQ ID NO.1 in a sequence table or is coded by a base sequence as shown in SEQ ID NO.2 in the sequence table. Therefore, a cat astrovirus indirect ELISA antibody detection kit is developed and comprises a coated ELISA plate, and the coated ELISA plate takes the recombinant CapA protein as a coating antigen. Meanwhile, a corresponding indirect ELISA antibody detection method is established. Research results show that the critical value of the indirect ELISA antibody detection method and kit is 0.523; the sensitivity is 1: 32000; the in-batch variation coefficient of detected samples is 2.679%-7.910%, the inter-batch variation coefficient is 3.627%-8.982%, both the in-batch variation coefficient and the inter-batch variation coefficient are smaller than 10%, and the clinical application effect is good. Therefore, the kit has the characteristics of high sensitivity, strong specificity, good repeatability and the like, can quickly and effectively detect the FAstV antibody, and provides technical support for accurate diagnosis, timely prevention and control and vaccine effect evaluation of FAstV.

The present invention discloses a genetically engineered bacterium expressing oligosaccharide debranching enzyme and its application, belonging to the technical field of microbial engineering. The present invention provides an oligosaccharide debranching enzyme with a specific base sequence, and successfully heterologously expresses the oligosaccharide debranching enzyme in Escherichia coli. By using the method of the present invention, the catalytic activity of the crude enzyme solution of the expressed oligosaccharide debranching enzyme can reach 258.83 U / mL. The oligosaccharide debranching enzyme provided by the present invention is a novel oligosaccharide debranching enzyme from a new source, which has not been reported before, has a high specific enzyme activity, and strong substrate specificity. The oligosaccharide debranching enzyme of the present invention can debranch short chain segments with DP<6 in maltodextrin, filling the blank in the substrate specificity of commonly used debranching enzymes at present, and has high potential application prospects in industrial fields such as starch sugar, resistant starch, beer production and ethanol fuel.

To develop a molecular marker related to carotenoid accumulation for the Patinopecten yessoensis variety "Haidu Jinbei" and to provide a method for maintaining population quality.SOLUTION: An InDel molecular marker related to carotenoid accumulation of Patinopecten yessoensis and an application thereof are disclosed. A base sequence of the InDel molecular marker in carotenoid accumulating Patinopecten yessoensis and a base sequence of the InDel molecular marker in common Patinopecten yessoensis are disclosed. Furthermore, an upstream primer sequence and a downstream primer sequence of a primer pair for amplifying the InDel molecular marker related to carotenoid accumulation of Patinopecten yessoensis are disclosed. By detecting the InDel molecular marker, homozygous Patinopecten yessoensis individuals capable of accumulating carotenoids can be conveniently and rapidly screened in a seedling stage and from parents, and effective technical support is provided for breeding of "Haidu Jinbei" and optimization of characters.SELECTED DRAWING: Figure 2

The invention discloses an mRNA sequence scoring method and related equipment. The method comprises the following steps: acquiring an mRNA sequence to be scored; extracting a key base sequence from the mRNA sequence, wherein the key base sequence comprises an initiation codon context and a termination codon context; inputting the key base sequence into a target scoring model, and processing to obtain a score of the mRNA sequence in the target index; wherein the target scoring model comprises a multi-modal RNA sequence coding module and a large language model, and the target scoring model is obtained based on unsupervised pre-training of transcription sample data and supervised fine tuning of a score label data set corresponding to the target index. According to the method, a non-coding region and a coding region key region (start / stop codon context) are integrated as input, and a large language model trained based on mass data is used for processing, so that various complex factors such as sequence context information and secondary structures can be comprehensively considered, and the method can be widely applied to the technical field of gene sequence analysis.