203 results about "Base sequence" patented technology

The invention discloses SSR (simple sequence repeat) multiple PCR (polymerase chain reaction) primers for paternity test of grass carp and application of the SSR multiple PCR primers. The primers comprise 16 pairs of specific primers, and base sequences of the 16 pairs of specific primers are respectively shown as SEQ ID NO.1-32 in sequence. The invention further discloses a fish paternity test kit comprising the SSR multiple PCR primer, an SSR multiple fluorescent PCR method for grass carp paternity test and application of the SSR multiple PCR primer, the kit or the method in grass carp paternity test. According to the kit, 16 pairs of specific SSR primers, multiple fluorescent PCR and a universal amplification primer technology are combined, 16 sites can be detected at a time, universal primers can be repeatedly used, and compared with simple single-site detection, the efficiency is improved, and the cost is reduced; the microsatellite loci contained in the invention are 3-6 basic groups, the allele size interpretation is more accurate, and the accuracy of genotype data is improved.

The invention discloses PDRN and a preparation method and application thereof.The base sequence of the PDRN is shown as SEQ ID NO1, and the preparation method of the PDRN comprises the following steps that fermentation treatment is conducted through lactobacillus plantarum, and thalli are collected after an obtained fermentation culture solution is centrifuged; and resuspending the obtained thalli, homogenizing and crushing, centrifuging to remove precipitate, concentrating by using a 5kDa hollow fiber membrane, purifying the obtained concentrated solution by using a Q column, and then concentrating and changing the solution to obtain the high-purity PDRN. According to the method, the PDRN is obtained through fermentation of lactobacillus plantarum for the first time, compared with PDRN sourced from salmon or salmon and the like at present, the method is lower in production cost, and a new way is provided for PDRN production. The PDRN obtained by the method disclosed by the invention is high in purity and single in molecular weight, and experiments prove that the PDRN obtained by the method disclosed by the invention has higher activity in the aspects of promoting cell proliferation, promoting collagen generation and the like.

A composition of matter includes an antisense phosphorodiamidate morpholino oligomer (MO) that includes an MO base sequence. The MO base sequence is arranged to bind a corresponding complementary base sequence of messenger RNA (mRNA) transcribed from one or more genes for the beta subunit of human chorionic gonadotropin (hCG-β). An inventive method, for suppressing intracellular synthesis of a beta subunit of human chorionic gonadotropin (hCG-β), includes introducing such an MO into one or more cells.

The invention discloses SSR (Simple Sequence Repeat) multiple PCR (Polymerase Chain Reaction) primers for megalobrama amblycephala paternity test and application, the primers comprise seven pairs of specific primers, namely a primer pair MamGLB2501, a primer pair MamGLB2502, a primer pair MamGLB2503, a primer pair MamGLG2504, a primer pair MamGLG2505, a primer pair MamGLR2506 and a primer pair MamGLR2507, and the base sequences of the seven pairs of specific primers are sequentially shown as SEQ ID NO.1-14; the invention also discloses an SSR multiplex fluorescent PCR method for megalobrama amblycephala paternity test by using the primer and application of the primer or the method in megalobrama amblycephala paternity test. The microsatellite site amplified by the primer is 3-6 base repetition, the typing is accurate, and the polymorphism of the amplified site is high; the method is accurate in identification and low in price.

This invention discloses an efficient RNA data preprocessing method to address the problems of low processing efficiency and low information density in high-throughput sequencing data. Its core steps include: (1) introducing a parallel processing scheme for high-throughput sequence data, rapidly mapping RNA-seq data to a reference genome to generate a BAM file; (2) extracting base sequences and expression levels and storing them as compact PKL format files; (3) extracting all exon position information by parsing the genome annotation file; (4) combining multi-sample expression level data to screen for highly variable exons and constructing a high-information-density feature list based on the sample set; and (5) accurately extracting target sequences from the preprocessed file based on this list. Compared to traditional methods, this innovative approach achieves triple optimization: full-process parallel processing for accelerated computation, high-compression data storage, and adaptive feature selection. Processing speed is increased by 3-5 times, and data volume is reduced by more than 90%, making it suitable for high-throughput RNA-seq data analysis with large sample sizes.

The present application relates to the technical field of plant genetic breeding, and particularly relates to a Brassica campestris fatty acyl reductase BcFAR2 gene, an expression protein thereof and application, a base sequence of the Brassica campestris fatty acyl reductase BcFAR2 gene is shown as SEQ ID NO. 1, and an amino acid sequence of the expression protein is shown as SEQ ID NO. 2, the Brassica campestris fatty acyl reductase BcFAR2 gene is cloned, and BcFAR2 gene overexpression Brassica campestris sterile plants can restore pollen activity; gene knockout Brassica campestris fertile plants can significantly reduce pollen activity; in Arabidopsis thaliana, overexpression of the gene can significantly improve salt tolerance of the transgenic plants; and in Brassica campestris, overexpression of the gene can significantly enhance salt tolerance.

The invention provides CrRNA for combined detection of chikungunya virus (CHIKV) and dengue fever virus (DENV), the CrRNA comprises Cas12a-crRNA and Cas13a-crRNA, and the base sequences of the Cas12a-crRNA and the Cas13a-crRNA are respectively shown as SEQ ID NO.1 and SEQ ID NO.2. The invention also provides a kit for detecting the chikungunya virus (CHIKV) and the dengue fever virus (DENV). The invention also provides a CRISPR-Cas system comprising the crRNA and application of the CRISPR-Cas system, and also provides a rapid detection method for chikungunya virus and dengue virus. According to the present invention, by designing the specific Cas12a-crRNA and the specific Cas13a-crRNA, the CRISPR-Cas dual system fused with the RT-RAA is constructed, such that the efficient amplification and the accurate identification are achieved; a fluorescent probe or an immunochromatography strip is combined to read signals, sensitivity and simplicity are taken into consideration, rapid combined identification of the two viruses is achieved, the kit is adaptive to multiple scenes, and a support is provided for early diagnosis and epidemic situation prevention and control.

This invention discloses a method, apparatus, and device for predicting the activity of chemically modified siRNA, comprising: acquiring the original base sequence and chemical modification information of the target siRNA, and retrieving the physicochemical properties of the target siRNA; encoding the original base sequence, chemical modification information, and physicochemical properties as features; and generating a prediction result of the silencing efficiency of the target siRNA based on the encoded features using a pre-constructed prediction model; wherein the pre-constructed prediction model includes: a feature fusion sub-model and a classification sub-model; the feature fusion sub-model is used to perform feature fusion on the encoded features based on a cross-attention mechanism; and the classification sub-model is used to generate a prediction result of the silencing efficiency based on the fused features. This method, based on a multi-dimensional, multi-view learning strategy and an attention mechanism fusion model, significantly improves the algorithm's ability to represent chemically modified siRNA data, and, combined with the nonlinear data fitting ability of the deep learning framework, improves the accuracy of the algorithm in predicting the drug activity of chemically modified siRNA.

The invention relates to the technical field of agricultural breeding, and discloses a molecular marker related to egg shell color and application of the molecular marker, the SNP molecular marker corresponds to the 18378867 site from the 5'terminal on a chicken reference genome GRCg6a 6 # chromosome published in an Ensembl gene browser database, and the base of the site is C / T and is missense mutation on an ABCG2 gene. The SNP molecular marker is related to the egg shell color, is a new molecular marker, can accelerate the breeding progress of the character by detecting the genotype of the locus of a chicken individual to be determined and performing early selection on the chicken with the dominant genotype, and has great application value and economic benefit.

The purpose of the present invention is to provide a novel mutant gene that imparts perpetual flowering properties to roses. Specifically, the present invention pertains to a KSN mutant gene comprising a base sequence (a) or (b): (a) a base sequence represented by SEQ ID NO: 1; and (b) a base sequence that is at least 90% identical in sequence to the base sequence represented by SEQ ID NO: 1, and maintains a transposon comprising the base sequence from position 729 to position 5812 in the base sequence represented by SEQ ID NO: 1.

The application relates to the field of biological medicine, and particularly relates to application of a TMEM119 gene or protein in development of an anti-gastric cancer drug, and particularly to application of a TMEM119 inhibitor in preparation of an anti-gastric cancer drug, wherein the inhibitor can be a small-molecule nucleotide for targeted knockdown of the TMEM119 gene or protein, the small-molecule nucleotide is shRNA or siRNA, the shRNA is a double-stranded oligonucleotide composed of complementary base sequences shown in SEQ ID NO. 1-2 or SEQ ID NO. 3-4; and the siRNA has a guide strand with a base sequence shown in SEQ ID NO. 7 or SEQ ID NO. 8. Experiments prove that when the expression of the TMEM119 gene of human gastric cancer cells is knocked down, the viability and the colony formation ability of the human gastric cancer cells are significantly reduced, thereby inhibiting the proliferation and survival of the gastric cancer cells, and further achieving the effect of restraining the occurrence, development, recurrence or metastasis of the gastric cancer.

The invention relates to the technical field of fish monitoring, in particular to a rare fish nondestructive monitoring method and system based on water eDNA sample recognition. A hotspot model is constructed through historical eDNA sampling distribution, sampling hotspot transfer prediction is achieved, and the optimal sampling point position is determined; high-throughput sequencing and a base sequence recognition decision tree are combined, the sequence rare degree is judged, and valuable and rare fishes are retrieved; further coupling the water flow dynamic model and the eDNA migration state space model, and tracing the potential migration area or inhabitation area of the rare fish; and a dynamic population community graph is constructed based on the time sequence community change, and the seasonal scale trend is analyzed. According to the method, accurate nondestructive monitoring of seasonal distribution rules and community structure trends of the rare fishes is realized on the basis of efficient collection and accurate identification of the eDNA of the water body, and a reliable scientific basis is provided for resource protection of the rare fishes in different water areas.

This invention belongs to the field of biodetection technology, specifically relating to a primer set, kit, and application for detecting *Alternaria solani* brown spot disease. The base sequences of the primer set are shown in SEQ ID NO.1~SEQ ID NO.5. Based on the primer set, this invention utilizes loop-mediated isothermal amplification technology for the rapid detection of *Alternaria solani*, accurately detecting the fungus from the complex pathogenic environment in diseased plant tissues. The rapid detection system described in this invention exhibits high specificity and sensitivity, with a detection limit of 0.246 fg / μL for *Alternaria solani* DNA. Using the rapid detection system provided by this invention, *Alternaria solani* can be detected rapidly, conveniently, accurately, and sensitively from field samples within 45 minutes, which is simpler and more efficient than traditional symptom identification methods and ordinary molecular detection methods.

The application discloses application of long-chain non-coding RNA LncZFHX2 in preparation of a drug for treating osteoarthritis. LncZFHX2 is specifically combined with KLF4 protein through a specific structure and a base sequence of LncZFHX2, and promotes transcriptional regulation of the KLF4 protein on RIF1 protein. LncZFHX2 indirectly regulates RIF1 protein to improve a cell state. Overexpression of LncZFHX2 inhibits osteoarthritis in vitro. In the results of the examples of the application, it is verified that overexpression of LncZFHX2 inhibits development of osteoarthritis, and a new intervention approach and a candidate drug are provided for clinical prevention and treatment of osteoarthritis. LncZFHX2 has a short sequence, does not need to be translated into a protein to play a role, has the characteristics of quick effect, high efficiency and low cost, and has high cost performance in synthesis and application.

The purpose of the present invention is to provide technology capable of accurately specifying a peak of a chromatogram that has been obtained by performing electrophoresis of a biological sample. A base sequence determination device according to the present invention fits a point spread function to a chromatogram, calculates a sigma estimation function that defines a standard deviation of the point spread function for each measurement point of the chromatogram, and estimates a correct standard deviation by searching for a peripheral region of the sigma estimation function (see fig. 13).

The present disclosure provides a technique for inhibiting ubiquitin ligase RFFL function and improving the plasma-membrane expression of a mutant CFTR protein. The present disclosure also provides a novel therapeutic agent for CF.　The present inventors have found that a modified oligonucleotide, or a salt thereof, having a specific base sequence and a modified structure, can inhibit ubiquitin ligase RFFL function and improve the plasma-membrane expression of a mutant CFTR protein.

The application provides a base sequencing quality evaluation method and system, which are used in the field of gene sequencing. The base sequencing quality evaluation method provided by the application comprises the following steps: providing a standard nucleic acid sequence, aligning the standard nucleic acid sequence to a reference sequence, and giving a label to a base in the standard nucleic acid sequence according to an alignment result; training a classifier to fit a relationship between a sequencing signal feature of the base and the label; providing a to-be-tested nucleic acid sequence, taking the base as a basic unit, and calculating the sequencing signal feature of the base in the to-be-tested nucleic acid sequence; and giving at least two quality values to the base in the to-be-tested nucleic acid sequence.

This invention relates to plant genetic engineering, and more particularly to a variegated Phalaenopsis orchid and its preparation method, the preparation method comprising the following steps: (1) according to the target gene with the base sequence shown in SEQ ID No. 1 PePDS (1) Synthesize specific nucleotide fragments; (2) Construct specific nucleotide fragments into a plant virus vector; (3) Introduce the recombinant plant virus vector obtained in step (2) into Phalaenopsis orchid plant material; (4) Screen to obtain Phalaenopsis orchids with silenced target genes. This invention achieves rapid, efficient, and non-transgenic leaf color variegation creation in commercial Phalaenopsis orchid varieties for the first time through VIGS technology; it not only breaks through the technical bottleneck of difficult and long cycle of genetic manipulation of orchid plants, significantly shortening the trait verification cycle, but also induces diverse and highly ornamental variegated leaf phenotypes such as albino, yellow, or purple in different varieties, and has the advantages of simple operation, low cost, and industrialization feasibility.

This invention provides a preventive and / or therapeutic agent for cystic kidney disease. The preventive and / or therapeutic agent for cystic kidney disease comprises a single-stranded oligonucleotide containing a base sequence complementary to at least a portion of the base sequence of miR-21.

The invention discloses a sequence alignment method and a seed search method and device thereof. The seed searching method comprises the steps that an index structure is constructed based on a reference genome sequence S, and the constructed index structure comprises an FM-Index index, a TBWT array, a TOCC array and a CNT array. And taking out the last base from the base fragments to be compared as an initial matching target, and locking the current matching region according to the CNT array and the initial matching target. And starting from the last but one basic group in the basic group fragment, matching two basic groups with S according to the current matching area and the constructed index structure in a reverse order every time until the number of the matched basic groups in the basic group fragment exceeds a preset threshold value, and taking the matched base sequence in the base fragment as a seed sequence. According to the method, the number of times of irregular memory access is reduced, so that the efficiency of a seed search task is improved, and a more efficient gene sequence comparison scheme is provided.

The present invention relates to a multiplex Sanger sequencing kit and method. Using the kit and method according to the present invention can facilitate multiple amplification and sequencing in Sanger sequencing for diagnosing genetic diseases and the like, and thus can significantly save costs and time.

The invention provides a DNA storage medium-oriented scalable vector graphics (SVG) image coding method and system, and the method comprises the steps: reading an SVG file, analyzing the SVG image to obtain a document tree containing a plurality of nodes, and distributing structure information for representing the structure position of the nodes in the document tree for the nodes; coding the nodes to generate node fragments, wherein the node fragments comprise label codes generated according to labels of the nodes and attribute codes generated according to attributes of the nodes; aggregating the plurality of node fragments according to the label codes thereof to form at least one aggregation block, the aggregation block comprising header information and the plurality of node fragments, the header information being used for indexing the node fragments contained therein; the at least one aggregate block is converted into at least one DNA base sequence, and error correction information for error verification or repair is added to the at least one DNA base sequence. According to the method, the encoding length is reduced while the semantic integrity of the SVG is maintained, the error-resistant capability is improved, and the progressive decoding capability is provided.

The application provides a tea plant gene and a method for regulating theanine accumulation in a tea plant CsSSCD1 The application belongs to the technical field of plant molecular biology. CsSSCD1 The base sequence of the gene is shown as SEQ ID No. 1. The application verifies the negative regulation of the gene by using VIGS technology and transient overexpression technology. In addition, through targeted amino acid metabolome analysis, it is found that CsSSCD1 The expression inhibition weakens the synthesis of tyrosine, the expression of the key enzyme AAAT of aromatic amino acid synthesis is inhibited, the accumulation of glutamic acid is reduced but not significant, and the accumulation of glutamine is significantly reduced, finally the degradation of theanine is blocked and the synthesis is increased. It is firstly proposed that the tyrosine degradation pathway may regulate the accumulation of theanine through metabolic pathway interaction and substrate competition mechanism, and on this basis, the key gene of the pathway is identified and functionally verified CsSSCD1 The gene is negatively correlated with theanine metabolism.

The present invention relates to a gene variant of the transcriptional regulator LysG and a method for producing L-citrulline or L-arginine using same. The gene variant of the transcriptional regulator LysG according to the present invention has protein activity that is altered due to the mutation of one or more bases in the base sequence of a gene encoding the transcriptional regulator LysG, and thus, it is possible to effectively produce L-citrulline or L-arginine from a recombinant microorganism comprising the variant.

The invention belongs to the technical field of DNA information storage, and relates to a dynamic information storage method based on a DNA condensation body and application thereof, the method comprises the following steps: coding file information into a DNA base sequence according to a coding rule, adding a cohesive end at a 3'end to obtain two complementary single-stranded DNAs, and synthesizing a DNA double-stranded structure with the cohesive end through co-annealing; the method comprises the following steps: synthesizing an X-type DNA structure by using four DNA single chains with cohesive ends at 3'ends through co-annealing; mixing the DNA double-chain structure with the X-type DNA structure, and incubating at room temperature in a salt ion solution to obtain a DNA aggregate containing DNA information; carrying out target information amplification and sequencing on the solution of the DNA condensation body containing the DNA information by utilizing an amplification primer, and decoding to obtain file information so as to realize reading of the file information; the DNA condensation body is used as a liquid-phase storage medium, and information reading and dynamic operation can be achieved.

A method for compressing molecular tagged sequence data includes: grouping sequence reads associated with a molecular tag sequence to form a family of sequence reads, corresponding vectors of flow space signal measurements and corresponding sequence alignments, calculating an arithmetic mean of the corresponding vectors of flow space signal measurements to form a vector of consensus flow space signal measurements, calculating a standard deviation of the corresponding vectors of flow space signal measurements to form a vector of standard deviations, determining a consensus base sequence based on the vector of consensus flow space signal measurements, determining a consensus sequence alignment and generating a compressed data structure comprising consensus compressed data, the consensus compressed data including for each family, the consensus base sequence, the consensus sequence alignment, the vector of consensus flow space signal measurements, the vector of standard deviations and the number of members.

The application discloses an mRNA sequence scoring method and related equipment, and the method comprises the following steps: acquiring an mRNA sequence to be scored; extracting a key base sequence from the mRNA sequence, wherein the key base sequence comprises a start codon context and a stop codon context; inputting the key base sequence into a target scoring model to obtain a score of the mRNA sequence on a target index; wherein the target scoring model comprises a multi-modal RNA sequence encoding module and a large language model, and the target scoring model is obtained based on unsupervised pre-training of transcription sample data and supervised fine-tuning of a score label data set corresponding to the target index. The application integrates non-coding regions and key regions of coding regions (start / stop codon contexts) as inputs, and processes them by using a large language model trained based on massive data, so that the application can comprehensively consider various complex factors such as sequence context information and secondary structure, and can be widely applied to the technical field of gene sequence analysis.

Provided is lacticaseibacillus paracasei or a variant thereof having 16S rRNA that includes a base sequence having a homology of at least 90% with the base sequence represented by SEQ ID NO: 1.

The present invention provides a single-stranded antisense oligonucleotide or pharmaceutically acceptable salt thereof for regulating the expression and / or function of the ATXN7 gene. In the single-stranded antisense oligonucleotide, each nucleotide is bonded by a phosphate group and / or modified phosphate group. The single-stranded antisense oligonucleotide includes a gap region, a 3' wing region bonded to the 3' terminal of the gap region, and a 5' wing region bonded to the 5' terminal of the gap region. The gap region is a deoxyribose-constituted nucleic acid in which a nucleic acid modified by a sugar moiety may be included. The 3' wing region and the 5' wing region are modified nucleic acids. The sugar modification constituting the single-stranded antisense oligonucleotide is a modified nucleic acid represented by formula (A1). The single-stranded antisense oligonucleotide has a base length of 12-30 mer. The base sequence of the antisense oligonucleotide is: a base sequence having a sequence identity of 90-100% with a base sequence that is complementary to at least one target region constituted at the same base length as the antisense oligonucleotide in the base sequence shown in SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, or SEQ ID NO: 5; a base sequence that is complementary to the base sequence obtained by deleting, substituting, inserting, or adding one or more bases in the target region; or a base sequence that, under stringent conditions, hybridizes with an oligonucleotide having the target region.

The present invention relates to the technical field of gene detection. Provided are a long-read and short-read co-sequencing device and a bioinformatics sequencing method. The method comprises: performing first base sequencing processing on a test object, so as to obtain a long base sequence of the test object; and performing second base sequencing processing on the test object, so as to obtain at least one short base sequence of the test object; and on the basis of the long base sequence and the at least one short base sequence, determining a target base sequence corresponding to the test object. Obtaining an accurate target base sequence while ensuring the base detection efficiency is achieved on the basis of maintaining the base detection efficiency of the acquiring of the long base sequence in combination with the base information accuracy of the short base sequence, such that the target base sequence can conform to the actual situation of the test object.