This invention discloses a low-temperature sterilization method for feed based on the synergistic effect of a complex bioenzyme, comprising the following steps: a pretreatment stage; a dynamic reaction stage: under conditions of 40-50℃ and 60%-70%
humidity, the
enzyme-catalyzed reaction lasts for 30 minutes, decomposing the microbial structure; and residual
enzyme activity is inactivated by short-term pulsed
ultraviolet light. The complex enzymes include
lysozyme, thermophilic
protease, and
lipoxygenase, and the nano-
chitosan carrier is mixed with the complex enzymes at a
mass ratio of 1:1. This invention avoids the destruction of heat-sensitive nutrients by operating at low temperature (40-50℃), achieves broad-spectrum sterilization by utilizing the synergistic effect of the complex enzymes, covering various microorganisms such as
bacteria, molds, and spores, and simultaneously uses
ultraviolet inactivation technology to ensure no chemical residues, protecting
animal health and
environmental safety. This method has low equipment cost, is easy to operate, and has no public acceptance issues, providing a green, efficient, and economical solution for the
feed processing industry, significantly improving the efficiency and safety of feed sterilization technology.