17 results about "Lipoxygenase" patented technology

This invention relates to the field of chicken gizzard lining processing technology, and particularly to an active polypeptide of chicken gizzard lining, its preparation method, and functional products. The preparation method includes the following steps: mixing chicken gizzard lining powder with water and subjecting it to alkaline heat treatment to form a slurry; sequentially adding Bacillus licheniformis alkaline protease, trypsin, and pepsin to the slurry for three separate hydrolysis processes to obtain a hydrolysate; after enzyme inactivation treatment of the hydrolysate, adding Lactobacillus plantarum for fermentation to obtain a fermentation broth; sterilizing the fermentation broth and then filtering it to obtain a fermentation supernatant; sequentially subjecting the fermentation supernatant to membrane filtration, sterilization, and spray drying to obtain the active polypeptide of chicken gizzard lining. The active polypeptide of chicken gizzard lining prepared by the method of this invention exhibits alcohol dehydrogenase activating activity, AGEs inhibitory activity, albumin denaturation inhibitory activity, lipoxygenase inhibitory activity, pancreatic lipase inhibitory activity, and a beneficial bacteria proliferation promoting effect.

A soybean milk enzyme deactivation and fishy smell removal treatment device belongs to the technical field of soybean enzyme deactivation and fishy smell removal treatment devices, and comprises a skin breaking device and a fishy smell removal device, the lower end of the skin breaking device is fixedly provided with the fishy smell removal device, and the skin breaking device is communicated with the fishy smell removal device. The problems that in the prior art, when soybean milk powder is produced, soybeans need to be treated, and due to the effect of lipoxygenase, typical beany flavor can be generated can be solved. And the beany flavor substance and the water-soluble protein have strong affinity and are difficult to remove.

The invention provides black powder yeast and application of the black powder yeast in preparation of tobacco products. The invention relates to a saccharomyces uniguttatus strain SC-8, which is preserved in China General Microbiological Culture Collection Center (CGMCC) on April 16, 2025, the address of the saccharomyces uniguttatus strain SC-8 is No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number of the saccharomyces uniguttatus strain SC-8 is CGMCC No.34232. The saccharomyces uniguttatus strain SC-8 is named as the saccharomyces uniguttatus strain SC-8. The strain can secrete lipoxygenase and degrade carotenoid and lipid substances in tobacco leaves. When the compound is used for enhanced fermentation of tobacco leaves, the content of carotenoid degradation products, cembrane degradation products, Maillard reaction products, chlorophyll degradation products, terpenoid substances and the like in the tobacco leaves can be remarkably increased, the total content of aroma components is increased by 21.2%, and the potassium-chlorine ratio is increased by 35.4%. The cigar tobacco essence can significantly improve the aroma amount, aroma richness, maturity, sweetness and combustibility of cigar tobacco leaves, reduce irritation and offensive odor, and highlight the aroma characteristics of sweet aroma, costustoot, bean aroma and the like, thereby improving the usability of the tobacco leaves.

The invention discloses low-fishy-smell high-satiety composite soybean milk and a preparation method thereof, and the composite soybean milk comprises the following components in parts by mass: 100 parts of a bean composition which is composed of soybeans, black beans, kidney beans, cowpeas and chickpeas in equal mass ratio; 3-5 parts of chia seeds; 1-2 parts of soybean lecithin; 0.5 to 0.8 part of a sweetening agent, wherein the sweetening agent comprises erythritol and mogroside; the metal ion chelating agent comprises sodium citrate and sodium phytate in a mass ratio of 2: 1; and 0.02 to 0.08 part of sodium chloride. The method has the beneficial effects that the activity of lipoxygenase in bean compositions after germination is effectively reduced and the beany flavor in soybean milk is reduced by virtue of a synergistic means of pulse and germination, and various bean compositions are arranged, are subjected to enzymolysis and are matched with chia seeds, so that the bean flavor in the soybean milk is reduced. The chia seeds and enzymatic hydrolysate of the bean composition can form a composite gel system, and the satiety of the soybean milk is remarkably improved.

The present invention discloses a method for regulating the activity of endogenous enzymes in soybeans to increase the oil yield and prepare a highly emulsifiable odorless soy protein isolate, which belongs to the field of food processing technology. The present invention mainly includes heat shocking soybeans, soaking them in a complex enzyme to control the water absorption rate, a second heat shock and drying at a controlled speed and temperature, so as to achieve the multiple purposes of destroying the cell wall structure to accelerate the water absorption of seeds, regulating the activity of endogenous enzymes in soybeans and in situ enzymatic hydrolysis of oil body proteins, and the like. The new soybeans thus obtained are subjected to conventional oil extraction to significantly improve the oil yield. In addition, the step-by-step heat shock treatment activates the activity of endogenous proteases in soybeans to the maximum extent, and by regulating the relationship between the intensity and time of heat shock and the water content in soybeans, while inhibiting the activity of lipoxygenase, the advantages of the functional characteristics of the dual-modified soy protein isolate by the in situ enzyme method of the endogenous enzyme system and the free enzyme method are fully utilized to prepare a highly emulsifiable odorless soy protein isolate product.

This invention discloses a low-temperature sterilization method for feed based on the synergistic effect of a complex bioenzyme, comprising the following steps: a pretreatment stage; a dynamic reaction stage: under conditions of 40-50℃ and 60%-70% humidity, the enzyme-catalyzed reaction lasts for 30 minutes, decomposing the microbial structure; and residual enzyme activity is inactivated by short-term pulsed ultraviolet light. The complex enzymes include lysozyme, thermophilic protease, and lipoxygenase, and the nano-chitosan carrier is mixed with the complex enzymes at a mass ratio of 1:1. This invention avoids the destruction of heat-sensitive nutrients by operating at low temperature (40-50℃), achieves broad-spectrum sterilization by utilizing the synergistic effect of the complex enzymes, covering various microorganisms such as bacteria, molds, and spores, and simultaneously uses ultraviolet inactivation technology to ensure no chemical residues, protecting animal health and environmental safety. This method has low equipment cost, is easy to operate, and has no public acceptance issues, providing a green, efficient, and economical solution for the feed processing industry, significantly improving the efficiency and safety of feed sterilization technology.

This disclosure provides a method of preparing a liquid soy base, which includes the steps of: roasting whole, dehulled soybeans to a maximum temperature sufficient to inactive lipoxygenase in the soybeans; milling the roasted soybeans to a particle size from about 120 microns to about 180 microns, alternatively from about 130 microns to about 170 microns; adding the milled roasted soybeans to water to reach a desired solids content to create a milled soybean and water mixture; heating the milled roasted soybean and water mixture to pasteurize or sterilize the milled roasted soybean and water mixture and thereby generate a liquid blend; and homogenizing the liquid blend. The disclosure also provides liquid soy bases produced by the methods.

The present invention provides a mutant of soybean lipoxygenase LOX2 and its application in creating odorless soybeans. The mutant is based on the parent amino acid sequence of SEQ ID NO.1. By introducing succinylation modifications at lysine residues 257, 263, and 472, the catalytic efficiency of LOX2 is reduced, reducing the oxidation of polyunsaturated fatty acids and effectively creating odorless soybeans. Compared with the wild type, the catalytic efficiency is reduced by 15.62%, providing a new basis for application in the food industry. In addition, by achieving molecular-level transformation through grafting breeding technology, the present invention not only improves soybean quality but also significantly reduces production costs, which is of great significance for soybean variety improvement and oil quality improvement.

The invention relates to a production process of a composite bean product. The production process comprises the following process steps: step 1, screening and cleaning soybeans; step 2, soaking: soaking the soybeans in water at a certain temperature, and adding cellulase into the water; step 3, crushing and pulping: crushing and pulping the soaked soybeans, adding a defoaming agent into the soybeans, and performing separation treatment on the ground primary pulp for multiple times to obtain soybean milk and bean dregs; step 4, boiling the soybean milk and filtering: carrying out lipoxygenase inactivation treatment on the soybean milk, then heating and cooking the soybean milk thoroughly, and after boiling, waiting until the soybean milk is cooled to 60-75 DEG C; step 5, curdling: curdling is carried out for many times by adopting a ternary compounding agent, curdling is carried out by adopting a dynamic stepped coagulator adding method during curdling, and a three-stage stirring process is further included, and step 6, after standing is carried out to form uncongealed bean curd, processing of bean products such as soft bean curd, bean curd sheets, hard bean curd and white dried bean curd is carried out respectively. And various bean products can be produced on one production line.

The present application belongs to the technical field of biological medicine, and particularly relates to an oral preparation of lipoxygenase of a healthy donor treatment type intestinal flora exosome package and a preparation method and application thereof. The oral preparation of lipoxygenase of a healthy donor treatment type intestinal flora exosome package comprises 0.18-0.78wt% lipoxygenase, 0.24-0.48wt% fucoidan, 1.0-2.0wt% tetrahydropyrimidine and a healthy donor treatment type intestinal flora exosome supplementing to 100% according to weight percentage. The present application adopts an ultrasonic method to prepare the lipoxygenase of a healthy donor treatment type intestinal flora exosome package, uses the treatment type intestinal flora exosome as a natural medicine carrier, successfully solves the technical problem of low oral bioavailability of lipoxygenase, and provides a new effective means for enhancing immunity and preventing and treating tumors.

The present invention relates to a Chlamydomonas insulata-derived 15S-lipoxygenase for simultaneously producing resolvin D5 and protectin Dx. More specifically, provided are: a composition for producing resolvin D5 and protectin Dx, the composition comprising, as an active ingredient, a Chlamydomonas insulata-derived 15S-lipoxygenase or a gene encoding same; a method for producing resolvin D5 and protectin Dx, the method comprising the step of treating a substrate with the composition; a recombinant expression vector for producing resolvin D5 and protectin Dx, the recombinant expression vector comprising the 15S-lipoxygenase gene; and a transformant in which a host cell is transformed with the recombinant expression vector.

The present disclosure describes that the 15-lipoxygenase (15-LO) product 4-hydroxynonenal (4HNE or 4-HNE) post-translationally modifies LRRK2 and is believed to induce a pathologically elevated, chronic LRRK2 kinase activity associated with a LRRK2 mediated disease or condition, e.g., Parkinson's disease or frontotemporal dementia. Production of LRRK2-4HNE adducts, e.g., LRRK2 Cys2024-4HNE and / or LRRK2 Cys2025-4HNE adduct described herein, may be an indication of a LRRK2 mediated disease or condition. Inhibiting the production of excessive 4HNE in cells by 15-LO inhibitors converted an elevated LRRK2 activity to a normal basal LRRK2 activity level as demonstrated in cellular experiments in the Examples. The 15-LO inhibition of 4HNE production is believed to only remove elevated LRRK2 activity associated with LRRK2 mediated diseases or conditions and not normal LRRK2 activity that may be required for other cellular functions. Accordingly, 15-LO inhibition of elevated LRRK2 activity and / or expression may afford a safe and effective treatment for LRRK2 mediated diseases or conditions.

The invention provides a method for stabilizing hemp seed oil emulsion by using xanthan gum. As one of more functional components developed in hemp seeds, the hemp seed oil body plays an important role in enhancing the comprehensive utilization of hemp seed byproducts and developing and processing hemp seed oil body foods. The oil body extract tends to be similar to emulsions or creams, and is a natural oil-in-water (O / W) emulsion. However, during the storage period, due to the effects of factors such as emulsion concentration, emulsifying components, endogenous substances and the like, the stability of the oil emulsion is finally influenced. In order to improve the stability of the emulsion, polysaccharide plant components contained in food are researched and applied. At present, polysaccharide as a plant component has been widely applied to the food industry. The stability of the oil body emulsion is influenced on the basis of a mechanism that polysaccharide changes the surface charge quantity and surface tension of an oil body, forms a non-covalent bond, passivates the activity of lipoxygenase and the like. By adding the xanthan gum, the physicochemical properties of the hemp seed oil emulsion are influenced, so that the formed more stable hemp seed oil emulsion has important significance in the food industry.

The invention belongs to the technical field of medicines for treating inflammatory skin diseases, and discloses application of kinsenoside in preparation of medicines for treating skin inflammatory diseases, the structural formula of kinsenoside is shown in the specification, and kinsenoside can be particularly used for preparing medicines for treating psoriasis. According to the application, the kinsenoside is applied to the preparation of the medicine for treating the skin inflammatory diseases, particularly the medicine for treating the psoriasis for the first time, the kinsenoside can up-regulate arachidonate 15-lipoxygenase (ALOX15) in the skin so as to intervene the psoriasis, and a candidate medicine is provided for clinically developing natural products for treating the skin inflammatory diseases including the psoriasis.

The invention relates to a wall breaking machine, a control method and device thereof and computer equipment. The method comprises the following steps: in response to a wall breaking machine starting instruction, identifying the type of to-be-processed food; if the type of the food to be processed is beans, a blue light source irradiation function is started; if the type of the to-be-processed food is not beans, identifying whether the type of the to-be-processed food is a vitamin C-containing type; and if the type of the food to be processed is the vitamin C-containing type, starting a red light source irradiation function. Thus, by identifying the type of the food to be processed, when the food is determined to be beans, the irradiation function of the blue light source is started, the blue light source starts the activity of targeted activation lipoxygenase, and the content of hexanal and hexanol products is reduced, so that the beany flavor is removed; when the beans are determined to be non-beans and are judged to contain vitamin C, the irradiation function of the red light source is started, and the red light source excites Cu / Zn-superoxide dismutase in a targeted manner, so that the generation amount is reduced, ascorbic acid oxidase is inhibited, and the vitamin C is further protected from being damaged.

The present disclosure provides compounds, compositions comprising them, and pharmaceutical uses thereof, for example, compositions suitable for the treatment of 12 lipoxygenase (12-LOX) mediated diseases.

The invention relates to a baking technology of fragrant tea leaves, and belongs to the technical field of tea leaf processing. In the tea leaf preparation process, supercritical CO2 extraction is used, activated carbon adsorption is used for effectively removing caffeine in tea leaves, meanwhile, activated carbon is soaked with an ethanol solution and then alternately soaked with acid and alkali, the removal efficiency of caffeine in the tea leaves can be improved, and the bitterness of the tea leaves is remarkably reduced; besides, cellulase, catalase and lipoxygenase are further selected for composite synergistic fermentation, the taste and fragrance of the tea leaves are obviously improved, the fermented leaves are subjected to fixation, rolling, baking, spreading and cooling and then mixed with the shaddock peel to be baked, and the taste and fragrance of the tea leaves can be obviously improved.