54 results about "Endogenous enzymes" patented technology

The invention discloses a method for converting rutin into quercetin through biological catalysis, and belongs to the technical field of biological catalysis. The method comprises the following steps: mixing rutin and a solvent to obtain a quasi-solid rutin suspension; mixing the paraphlonia plant endogenous enzyme and the quasi-solid rutin suspension for enzymolysis, and then filtering, washing and drying to obtain quercetin; the solvent is methanol with the concentration of 5-50%, ethanol with the concentration of 5-50% or acetone with the concentration of 10-50%. The method is mild in biological catalytic reaction condition, does not generate pollutants such as acid waste liquid, and meets the environmental protection requirement. Efficient conversion of quercetin is realized by adopting a quasi-solid rutin strategy (rutin suspension reaction system), and the problem of substrate solubility limitation is solved. High-purity quercetin can be obtained through simple washing, the product purity is larger than 95%, and food and clinical application can be met.

The invention discloses a fresh plant directed biotransformation method based on endogenous enzyme activation and a product and application thereof. The core of the preparation method is as follows: fresh plants (tuberous roots, stems or fruits) which are harvested in a ripe state and have metabolism in a dormant state (the respiration rate is lower than 5 mg CO / kg.h) are taken as raw materials, the raw materials are treated in an environment with the specific pressure being 0.02-0.20 MPa, the temperature being 70-121 DEG C and the humidity being 50-95%, a specific impregnating compound can be selectively combined, and the raw materials can be prepared into the plant culture medium. Under the conditions that exogenous microorganisms are not introduced and plant tissue structures are not damaged, the catalytic function of an endogenous metabolic enzyme system is safely and efficiently restarted and maximally promoted, and components of a plant body are driven to realize directional biotransformation. According to the process, conversion of primary metabolites and / or secondary metabolites is remarkably promoted, the content conversion of one or more beneficial components in the primary metabolites and / or the secondary metabolites is improved, and meanwhile, the biological and pharmaceutical structures and inherent metabonomics characteristics of the plants are completely reserved. The biotransformation product can be used as a high-quality raw material to be applied to medicines, common foods, health foods, special diet foods, daily chemical products and disinfection products, and can be further prepared into traditional Chinese medicine decoction pieces, solid preparations, semi-solid preparations and liquid preparations by adding or not adding pharmaceutically acceptable auxiliary materials.

The invention discloses a method for preparing rare saponin by utilizing panax japonicus endogenous enzyme, and relates to the technical field of extraction, the panax japonicus plant material or crude extract thereof is directly taken as a starting point, the enzyme system is simple and convenient to obtain (fresh medicinal material low-temperature homogenate extraction or direct use of freeze-dried powder), the reaction system is simple, and the operation is easy. According to the preparation process provided by the invention, the catalytic action of endogenous enzyme is fully utilized, so that the introduction of exogenous substances (acid-base, protein and the like) in the extract is completely avoided; the method disclosed by the invention is high in catalytic efficiency, can quickly realize complete conversion of saponin (within 10min) under specific conditions, and has extremely high industrial application.

This invention relates to a recombinant Halomonas strain that produces polyhydroxyalkanoates (PHA) by overexpressing endogenous enzymes encoding ADP-dependent acetyl-CoA synthase (ADP-ACS). acd This gene not only enhances the recombinant bacterium's tolerance to acetic acid but also enables it to efficiently convert acetic acid or acetate substrates into acetyl-CoA, thereby participating in the tricarboxylic acid cycle (TCA cycle) and the synthesis of PHA. The present invention also relates to a method for producing PHA using the aforementioned recombinant Halomonas bacterium employing a pH-stat strategy.

The invention discloses an enzyme-activated fluorescent molecular precursor as well as a preparation method and application thereof. The enzyme-activated fluorescent molecular precursor has a chemical structure as shown in a formula I which is described in the specification. The invention develops an enzyme-activated fluorescent molecular precursor which can be used for real-time imaging of tumors by targeting exogenous enzyme to the tumors and specifically activating a probe at the tumors; according to the invention, the exogenous enzyme is introduced as an activation trigger, and normal cells do not have the exogenous enzyme, so that the problem of co-expression of the endogenous enzyme in non-tumor tissues can be avoided, background fluorescence signals can be reduced to the greatest extent, and the imaging specificity and accuracy are remarkably improved.

The invention provides an antheraea pernyi fresh-keeping method which comprises the following steps: S1, dividing a first group and a second group with different freezing response characteristics according to development biological characteristics of antheraea pernyi; the freezing response characteristic is represented by at least one parameter of tissue moisture content, methylene blue penetration depth and body wall elasticity modulus; s2, differential preprocessing is applied to the first group and the second group, so that the difference of the first group and the second group on any characterization parameter does not exceed a preset threshold value; s3, combining the two groups of tussah and freezing; in the first cooling stage, cooling from 0-1 DEG C to-10 DEG C at the speed of 0.8-1.2 DEG C / min; in the second cooling stage, cooling is conducted from-10 DEG C to-30 DEG C at the speed of 0.45-0.55 DEG C / min; in the third cooling stage, cooling from-30 DEG C to-45 DEG C at the speed of 1.8-2 DEG C / min; and S4, after freezing is completed, vacuum packaging is performed, and storage is performed at minus 45-minus 40 DEG C. According to the fresh-keeping method for the tussah, the integrity of a cell structure is maintained, juice loss is reduced, and abnormal release of endogenous enzyme is inhibited, so that nutritional ingredients are better reserved in the long-term freezing storage process, and the sensory characteristics of compact meat, uniform color and luster and no peculiar smell are maintained.

The invention relates to a method of producing an ester of a fatty acid and a C1-C4 alcohol, comprising successive steps of: cultivating cells of a microalga strain on a growth medium, harvesting the cultivated cells and concentrating them to obtain a wet cell pellet, suspending said wet cell pellet in an hydroalcoholic solution comprising said C1-C4 alcohol, incubating the cell suspension thus formed so as to form said ester of a fatty acid and a C1-C4 alcohol by transesterification and / or esterification of lipids produced by the microalga cells catalyzed by endogenous enzymes of said cells, and recovering said ester of a fatty acid and a C1-C4 alcohol from said cell suspension. The growth medium can advantageously be a dark fermentation effluent.

The present application relates to a freshness indicator protein related to quality traits of low-temperature stored Siniperca chuatsi, endogenous proteases and applications. The freshness indicator endogenous proteases are related to postmortem proteolysis and meat softening of Siniperca chuatsi, including cathepsin B, cathepsin L and calpain. The freshness indicator proteins are related to quality changes occurring during postmortem cold storage of Siniperca chuatsi, including myoactin 1, myosin binding protein, LDB protein, actin-related protein, etc. The present application determines the change rule of endogenous protease activity of Siniperca chuatsi at different storage stages, and the dynamic influence on muscle proteolysis and muscle degradation of Siniperca chuatsi, and reveals the internal relationship with fish meat sensory quality and processing characteristics, and clearly defines the "critical point" of quality deterioration of Siniperca chuatsi. It provides clear guidance for the control of endogenous enzyme-mediated quality deterioration in the processing and storage process of Siniperca chuatsi.

The invention belongs to the technical field of food processing, and particularly relates to a garlic pickling method capable of promoting nutrient absorption, which comprises the following steps: S1, performing micropore puncture pretreatment on carefully selected complete garlic bulbs to form through pore channels in specific directions so as to establish rapid permeation channels of subsequent treatment substances; s2, sequentially immersing the garlic bulbs punctured by the micropores into a low-concentration and low-temperature first ethanol solution and a high-concentration and high-temperature second ethanol solution for gradient ethanol dehydration, then carrying out heat treatment to remove part of water, carrying out primary sterilization and activating the endogenous enzyme activity of the garlic bodies. According to the invention, physical pretreatment, gradient dehydration, heat treatment activation and compounding of absorption-promoting pickling liquid are combined with a dynamic alternate pickling process to synergistically destroy garlic cell barriers, activate endogenous enzyme conversion and introduce absorption-promoting functional components, so that the pickled garlic product with good flavor and nutrition easier to absorb by human bodies is prepared within a short time.

This invention relates to the field of grain processing technology, and in particular to a method for improving the nutritional value of brown rice and reducing its glycemic index. The invention uses brown rice as raw material, processing it through a process of mist-wetting for nutrient enrichment, integrated microwave gelatinization and drying, tempering, and milling to produce semi-cooked rice (parboiled rice). The brown rice processed by the method provided by this invention is clean and odorless, slows down the rate of water penetration, prevents grain cracking, reduces the rate of broken rice during subsequent milling, and, under the action of endogenous enzymes, enriches nutrients such as GABA, flavonoids, and free amino acids. Furthermore, the bran layer of the dried brown rice is easily separated.

This invention relates to the field of feed additive technology, specifically to a feed additive that promotes digestion and absorption and its preparation process. The preparation process of a feed additive that promotes digestion and absorption includes: preparing antioxidant-modified chicken fat; preparing an extract; preparing a composite oil phase; and preparing the feed additive. In this invention, the extract of Shenqu (a type of fermented wheat) can directly compensate for insufficient endogenous enzyme secretion in laying hens, rapidly decomposing starch in feed and chicken fat in plant proteins, laying the foundation for subsequent absorption. The extract of Zhishi (a type of immature bitter orange) accelerates gastrointestinal motility and digesta emptying, while also promoting bile and pancreatic juice secretion, further improving the digestibility and utilization rate of fat-soluble components in laying hens. The extract of pine pollen can repair the intestinal mucosa, increase intestinal villus height, increase the nutrient absorption area, and regulate the intestinal microecology, reducing nutrient loss and intestinal stress. The combined use of these three ingredients synergistically promotes the digestive and absorptive capacity of laying hens.

The present invention relates to a cleaning composition, preferably a cleaning composition for use in automatic dish washing and / or laundry washing, which cleaning composition comprises an Alkalitalea saponiacus endogenous enzyme or a variant thereof. The invention also relates to a method of cleaning using the composition of the invention and to the use of an enzyme in a cleaning composition.

This invention discloses a cooking utensil and a method for preparing sleep-enhancing factors enriched therein, belonging to the field of household appliances. The method for preparing sleep-enhancing factors enriched in the cooking utensil includes a stress enzymatic hydrolysis process and a baking process. The stress enzymatic hydrolysis process refers to pre-treating the ingredients to activate their endogenous enzymes. Pre-treatment includes at least one of the following: steam heating and humidification treatment, periodic heating-cooling treatment, low-temperature treatment, low-oxygen treatment, negative pressure treatment, high-pressure treatment, ultrasonic treatment, pulsed light treatment, and plasma treatment. The baking process refers to baking the ingredients treated with the stress enzymatic hydrolysis process. The preparation method of this invention can significantly increase the GABA content of the ingredients, while also improving the bitter taste and enhancing the aroma. In other words, this invention solves the problem that existing cooking methods cannot simultaneously achieve good taste and high nutritional value.

The disclosure relates to the field of chronic pain and the treatment thereof. The disclosure involves antisense oligonucleotides and the use thereof in pre-mRNA and mRNA modulation, in one aspect related to RNA editing using endogenous ADAR enzymes, in targeting an adenosine in a (pre-) mRNA for human Nav1.7, preferably to change from a lysine residue to an arginine residue in the protein at position 1406 (K1406R), thereby impairing the ability of the Nav1.7 protein to act as a sodium ion channel. In another aspect the disclosure relates to exon skipping wherein antisense oligonucleotides are used to induce skipping of exon 23 in the generation of human SCN9A mRNA thereby generating a shortened Nav1.7 protein with an impaired ability to act as a sodium ion channel. The disclosure relates to methods and means to alter the Nav1.7 protein in cells of the peripheral nervous system, preferably large nociceptor cells (type Aα / Aβ), to provide a treatment of chronic pain.

This invention discloses a food-medicine homology composition and its application in black bean products, belonging to the field of food processing technology. First, black beans are washed and frozen, then rinsed with warm water to obtain broken-cell black beans. The broken-cell black beans are then mixed with dried angelica slices and stacked to balance the moisture content and promote the migration of angelica components and endogenous enzymatic hydrolysis of the black beans, resulting in a mixture. Finally, the mixture is placed in a wok with rock sugar and stir-fried using a stepped heating and stirring process to allow the rock sugar to melt and penetrate into the cracks of the black beans and cause the seed coat to wrinkle, thus obtaining the food-medicine homology composition. This achieves a food-medicine homology product with high active ingredient content, strong antioxidant capacity, and excellent sensory quality.

The invention relates to the technical field of biological fermentation, and discloses a functional fermentation product preparation process based on directional conversion of bean dregs, and the preparation process comprises the following steps: firstly, performing autolysis pretreatment on the bean dregs through an osmotic pressure regulator, activating an endogenous enzyme system, and releasing soluble nutrient substances; then, in the solid state fermentation stage, a composite stress factor composed of short-chain inulin and sorbitol is added, aspergillus oryzae is induced to form a loose and porous hypha network skeleton, and the ventilation and mass transfer efficiency of a fermentation substrate is remarkably improved. Then, regulating and controlling a cooling time sequence, adding L-glutamic acid as a metabolism inducer, and accurately receiving a metabolism path of bacillus megaterium to realize directional enrichment of functional products. The method effectively solves the problem that the physical structure is not matched with microbial metabolism in solid fermentation of the bean dregs, and improves the yield of functional fermentation products and the resource utilization value of the bean dregs.

This invention belongs to the field of whole grain deep processing technology, and relates to a high-resistant starch whole grain powder and its preparation method. The raw materials of the high-resistant starch whole grain powder include the following components by weight: chickpeas 25-45 wt%, quinoa 20-30 wt%, brown rice 10-20 wt%, pea protein isolate 10-20 wt%, hemp seed protein 1-10 wt%, fructooligosaccharides 1-5 wt%, and compound nutrients 0.5-4 wt%. The high-resistant starch whole grain powder has a resistant starch content ≥35 (g / 100g starch), a GI value ≤55, and a powder particle size ≤100μm. This invention significantly increases the resistant starch content of the obtained whole grain to over 35% through the synergistic effects of germination activating endogenous enzymes, fermentation promoting starch molecule rearrangement, and aging-induced retrogradation. The product has a GI value ≤55, a natural fermented aroma, and a fine, easily reconstituted texture.

Provided is a mutated lysophospholipase. Further provided are a gene encoding the lysophospholipase, and a vector and a host cell comprising the gene. In addition, further provided is a use of the lysophospholipase. The present invention also provides a mutated Aspergillus niger strain and a use thereof. Specifically, the present invention provides a mutated Aspergillus niger strain, which is an orotate phosphoribosyltransferase auxotrophic strain, and has improved production capacity of endogenous enzymes, preferably lysophospholipase, with respect to an unmutated strain. The present invention further provides uses of the strain. For example, the strain can be used for expressing the mutated lysophospholipase of the present invention.

This invention discloses a method for the targeted conversion and synthesis of rare ginsenosides Rg3 and Rh2. Specifically, fresh Panax notoginseng leaves are microwave-dried for 1-3 minutes to inactivate their endogenous β-glucosidase. The dried Panax notoginseng leaves are extracted with anhydrous methanol, and the extract is resuspended in an acetate-sodium acetate buffer solution after drying. α-arabinofuranase, β-xylosidase, and β-glucosidase are added to the resuspended solution, and an enzymatic reaction is carried out at 35-40℃ to directionally convert the original ginsenosides into rare ginsenosides Rg3 and Rh2. This method can achieve the inactivation of endogenous enzymes and the stable retention of original saponins in Panax notoginseng leaves in a very short time. Compared with the natural drying combined with exogenous enzymes, the conversion ratios of Rg3 and Rh2 are increased by 3.14 and 2.4 times, respectively. Compared with fresh Panax notoginseng leaves, the contents of Rg3 and Rh2 are increased by 20.6 and 8.7 times, respectively. This method is suitable for the high-value utilization of Panax notoginseng leaf waste resources and the large-scale preparation of rare saponins.

The invention discloses an EPSF / polygonatum sibiricum polysaccharide / casein submicron composite preservative as well as a preparation method and application of the EPSF / polygonatum sibiricum polysaccharide / casein submicron composite preservative. The compound is prepared by compounding EPSF, polygonatum polysaccharide and sodium caseinate according to a mass ratio of 1: (0.2-0.8): (2-8), wherein the EPSF is synthesized by catalyzing an endogenous enzyme from aspergillus niger, and the compound is prepared by ultrasonic treatment and freeze-drying. The compound has excellent oxidation resistance, the superoxide anion clearance rate is as high as 88.36%, meanwhile, the compound preservative has broad-spectrum antibacterial activity, and the diameter of an inhibition zone is as high as 23.25-26.5 mm. When being applied to fresh-keeping of fruits and vegetables such as fresh-cut apples and potatoes, the fresh-keeping agent can remarkably inhibit browning, the browning index is reduced by about 60-80%, the weight loss ratio is reduced by 20-30%, and the fresh-keeping agent has the advantages of maintaining hardness, effectively prolonging the fresh-keeping period, being efficient, safe, nutritional and the like.

The invention discloses a preparation method of a sugar-inhibiting fermented mulberry leaf extracting solution, relates to the technical field of natural product extraction, and solves the technical problem of low extraction rate of DNJ in mulberry leaves. The method comprises the following steps: crushing fresh mulberry leaves, and carrying out re-freezing and re-thawing circulation; adding water into the mulberry leaf powder, inoculating a sugar-inhibiting eurotium cristatum seed solution, fermenting, centrifuging, and collecting supernate A and residues A; adding a compound enzyme 1 into the residue A for enzymolysis, adjusting the pH value, adding a compound enzyme 2 for enzymolysis, centrifuging, and collecting supernate B; combining the supernate A and the supernate B, and filtering to obtain the sugar-inhibiting fermented mulberry leaf extracting solution. According to the method, 1-deoxynojirimycin (DNJ) in mulberry leaves is efficiently enriched, the alpha-glucosidase inhibitory activity is improved, the obtained product can be used for developing blood sugar regulating functional foods or vegetable drugs, endogenous enzymes are activated through fermentation, tissue structures are softened, and the extraction rate of DNJ in the mulberry leaves can be increased.

The present application relates to a kind of fruit and vegetable probiotic fermentation integrated device based on multistage processing, belong to fermentation engineering technical field, each module in device is sequentially connected by closed material conveying pipeline and constitutes continuous production line;Material pretreatment module includes the breaking mechanism and enzyme inactivation mechanism connected in sequence, for preparing fruit and vegetable slurry and carrying out transient heat treatment inactivation endogenous enzyme;Heat energy recovery module is used to recycle the heat energy of high-temperature slurry after enzyme inactivation and supply subsequent unit;Fermentation substrate preparation module is used to accurately temperature control, low-temperature concentration after cooling inactivated slurry and uniformly mixed with probiotic liquid;Control module is connected with each mechanism communication, and the operating parameters and process timing are coordinated and controlled.The present application realizes seamless connection and energy reuse of high-temperature enzyme inactivation and low-temperature concentration by the synergistic effect of heat energy recovery and dynamic temperature control, optimizes probiotic growth conditions while protecting the heat-sensitive nutrients of fruit and vegetable, significantly improves product quality and production efficiency.

The invention discloses yarrowia lipolytica genetically engineered bacteria capable of producing decaisopentenol and application of the yarrowia lipolytica genetically engineered bacteria, and belongs to the technical field of bioengineering. The yarrowia lipolytica genetically engineered bacterium is obtained by cloning a decene diphosphate synthase gene into yarrowia lipolytica, overexpressing related genes of an endogenous acetyl coenzyme A synthetic pathway and a mevalonic acid pathway of the yarrowia lipolytica, weakening a squalene synthetic pathway and knocking out endogenous ylCOQ1 enzyme. Experiments prove that the yarrowia lipolytica genetically engineered bacterium can significantly improve the yield of decyl isopentenol, and the product serves as an important precursor for chemical synthesis of coenzyme Q10, so that the engineered bacterium and the product thereof can be applied to biochemical synthesis of coenzyme Q10. The method is green and efficient, and has important industrial application value.

This invention belongs to the field of egg product processing technology, and discloses a Maillard reaction-based flavor-enhancing egg liquid, its preparation method, and its application. The flavor-enhancing egg liquid of this invention is prepared from the following raw material components via a Maillard reaction: egg liquid, L-cysteine, and natural compound sugars. This invention, for the first time, synergistically activates endogenous cysteine ​​proteases in egg liquid by adding L-cysteine, achieving precise and targeted hydrolysis of large protein molecules in the egg liquid without the introduction of exogenous proteases. This provides sufficient precursor substances for the Maillard reaction, thereby significantly enhancing the natural egg aroma, caramel aroma, and other characteristic flavors of the egg liquid. This flavor-enhancing egg liquid has a naturally rich aroma, high integration with the matrix, and maintains good emulsification and fluidity. When used in the production of baked goods such as cakes, bread, and egg tarts, it can impart a rich and lasting natural egg aroma to the final product, while also improving the color and taste of the final product, showing promising prospects for industrial application.

The invention discloses a processing method of goose meat blocks with high nutritional value, which comprises the following steps: selecting fresh goose or frozen goose qualified by quarantine, unfreezing, cleaning, removing lymph and excess fat, and cutting into meat blocks with uniform specification. The papain is utilized to cut off muscle fibers under a specific pH value, and calcium chloride is matched to activate endogenous enzymes, so that the shearing force value of the goose meat blocks is reduced to about 30N, and the effect that the goose meat blocks are soft and rotten without losing shape is achieved; low-temperature tenderization and short-time high-temperature baking are combined, so that the damage of long-time high temperature to protein and vitamins is avoided, and the retention rate of the protein is greater than 95%; the grape wine and the Chinese herbal medicine extract are added, so that the fishy smell is removed, the product is endowed with unique medicine fragrance and fruit fragrance, the product has a natural anti-oxidation effect, and an artificially synthesized preservative does not need to be added; the process of steaming first and then baking enables the interior of the meat loaf to be well-done and juicy, the surface of the meat loaf is subjected to a Maillard reaction to form attractive golden yellow, and appetite is increased.

The present invention relates to an injectable delivery system for long-acting administration of drugs. The present invention includes compounds formed at least in part from peptidomimetic molecules. The compound is configured to link one or more drug molecules, and is further configured to release the one or more drug molecules under physiological conditions. A solution of the compound forms a hydrogel in response to an endogenous enzyme of a human or animal. A solution of the compound can be applied to the skin where it forms a hydrogel upon application, and any drug attached to the compound will then be gradually released.

The invention discloses a poultry meat tenderizing and freshness increasing method based on endogenous enzyme activation, and aims to solve the problems of non-uniform tenderizing and low efficiency due to dependence on exogenous additives in the prior art. The method comprises the following steps: firstly, carrying out pretreatment and stress regulation on poultry meat to obtain raw materials with consistent states; then inducing intracellular energy signal channel activation through intermittent mechanical stimulation and gas environment change; then establishing a microenvironment for activating endogenous calcium-activated protease by using a composite osmotic treatment liquid containing a natural calcium source and assistance of a physical field; then carrying out dynamic standing under the conditions of accurate temperature control, humidity control and specific gas, so as to promote the endogenous protease system to have a sufficient synergistic effect; and finally, carrying out gradient heating and rapid cooling to terminate enzyme activity and lock quality. According to the method, fundamental and uniform tenderization occurring in muscles is achieved, flavor precursor substances are synchronously increased, dependence on exogenous enzyme preparations is not needed, the method conforms to the label cleaning trend, and the processing efficiency is remarkably higher than that of a traditional ripening process.

The invention discloses a transmission type ozone micro-nano bubble coupling slurry ice bacteriostasis and enzyme inactivation system and application thereof.According to the transmission type ozone micro-nano bubble coupling slurry ice bacteriostasis and enzyme inactivation system, a flow guide pressing device is arranged above a conveying belt, and a flexible net is combined, so that the floating problem of aquatic products is effectively solved; a multi-direction stirring paddle and a side plug flow nozzle are introduced into the cooling tank, so that the heat transfer efficiency is improved, and the uniformity of the ozone effect is enhanced; the ozone micro-nano liquid stabilization technology is cooperated with the high-voltage pulse electric field, so that the ozone solubility and the action durability are remarkably improved. According to the method, the effects of broad-spectrum sterilization and endogenous enzyme activity delaying are achieved, rapid cooling of the pre-cooled water product can be achieved, and the shelf life is prolonged.

The invention belongs to the technical field of aquatic product processing, and particularly relates to a constant-temperature and constant-pressure soaking method for light dry sea cucumbers, which comprises the following steps: putting the light dry sea cucumbers in an integrated reaction kettle, soaking the light dry sea cucumbers in purified water, and introducing steam for heat shock treatment to inactivate endogenous enzymes and promote collagen denaturation and unwinding; a soaking stabilizer composed of biological polysaccharide, fish collagen peptide and protease is used for soaking at constant temperature and constant pressure, and remodeling of a collagen network is achieved under the condition that a hypotonic environment is maintained through the synergistic effect that polypeptide guides collagen folding, polysaccharide promotes system stability and biological enzyme covalent cross-linking; on one hand, material transfer loss and damage are avoided, the production efficiency is greatly improved, on the other hand, the problem that low-temperature gelation of sea cucumber collagen is unstable is solved, and the original ecological elastic and tough taste of sea cucumbers is maintained.

The invention discloses a low-temperature low-carbon water extraction-natural fermentation debitterizing method of Huyoshan Huyoshan pomelo, which comprises the following steps: extracting Huyoshan pomelo peel by using water as an extraction medium in combination with a physical superfine grinding and ultrasonic-assisted technology by adopting a water extraction method, so as to realize efficient extraction of naringin; then, further debitterizing by utilizing a natural fermentation debitterizing mode, specifically, placing an extracted clear solution subjected to ultrasonic treatment in a sealed environment, fermenting by utilizing an endophytic flora naturally attached to the grapefruit peel, degrading and converting a bitter substance naringin by virtue of microbial metabolism and an endogenous enzyme effect, and performing centrifugal separation after natural fermentation is completed, and supernate is removed to obtain debittered grapefruit peel powder, so that the bitter taste strength is further reduced, and the flavor is improved. The method has the advantages of being environmentally friendly, low in temperature, low in carbon, remarkable in debitterizing effect and the like, and the obtained extracting solution and the debitterized grapefruit peel powder both keep certain antioxidant activity and can be used for development of functional beverages, nutritional health-care products and seasoning products.