227 results about "Endogenous enzymes" patented technology

The present disclosure provides a device having a casing with a barrier surface and a contact surface and a composition in the casing having a nitric oxide precursor and an isolated enzyme or live cell expressing an endogenous enzyme, for converting the nitric oxide gas precursor to nitric oxide gas or having activity on a substrate that produces a catalyst that causes the conversion of the nitric oxide gas precursor to nitric oxide gas. The present disclosure also provides methods and uses for treating wounds, microbial infections and dermatological disorders and for preserving meat products.

The invention relates to a manufacturing method of fermented black garlic, which is characterized in that the temperature and the humidity in a fermentation room are controlled, so that the endogenous enzyme of the garlic infiltrated in inoculation liquid is activated, and the internal natural fermentation process of the garlic is finished in the fermentation room. The fermented black garlic produced by the manufacturing method can be stored to the utmost extent, the physiological activity of the garlic is increased, and the garlic has good mouthfeel and no strong biting taste.

The present disclosure provides a device having a casing with a barrier surface and a contact surface and a composition in the casing having a nitric oxide precursor and an isolated enzyme or live cell expressing an endogenous enzyme, for converting the nitric oxide gas precursor to nitric oxide gas or having activity on a substrate that produces a catalyst that causes the conversion of the nitric oxide gas precursor to nitric oxide gas. The present disclosure also provides compositions, methods and uses for skin cosmesis.

The invention relates to the technical field of biology, and discloses an oyster peptide with a sexual promoting function and a preparation method and application thereof. The method comprises the steps that oyster meat, calcium salt and water are ground to obtain oyster meat paste; enzymolysis is performed on the oyster meat paste, and centrifugation is performed to obtain supernatant after enzymolysis is performed, and an oyster enzymolysis coarse solution is obtained; discoloring and impurity removing are performed on the oyster enzymolysis coarse solution, and an oyster peptide refined solution is obtained; concentrating and spray drying are performed on the oyster peptide refined solution to obtain the oyster peptide. According to the oyster peptide with the sexual promoting function and the preparation method and application thereof, due to the fact that the oyster meat is pretreated with the calcium salt before enzymolysis is performed on the oyster meat, endogenous enzymes of oysters are activated, and enzymic preparations consumed in subsequent enzymolysis are reduced; meanwhile, the specific enzymic preparation composition is adopted to cooperate with the endogenous enzymes of the oysters to perform enzymolysis in two steps, and therefore the oyster peptide with the higher antioxidant activity and the significant sexual promoting function is prepared.

Provided herein are compositions and methods for improved production of acetyl-CoA and acetyl-CoA derived compounds in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a phosphoketolase (PK), and a functional disruption of an endogenous enzyme that converts acetyl phosphate to acetate. In some embodiments, the host cell further comprises a heterologous nucleotide sequence encoding a phosphotransacetylase (PTA). In some embodiments, the enzyme that converts acetyl phosphate to acetate is a glycerol-1-phosphatase. In some embodiments, the glycerol-1-phosphatase is GPP1 / RHR2. In some embodiments, the glycerol-1-phosphatase is GPP2 / HOR2. The compositions and methods described herein provide an efficient route for the heterologous production of acetyl-CoA-derived compounds, including but not limited to, isoprenoids, polyketides, and fatty acids.

The invention discloses a method for preparing bioactive protein powder through endogenous enzymolysis of marine low-value coarse fish, which is characterized by comprising steps of raw material preparation, primary crushing, enzymolysis, small peptide liquid preparation, mixing and drying, post treatment and the like. During enzymolysis, enzymolysis process parameters are selected according to the endogenous enzyme activity measured from the raw materials; and during mixing and drying, peeled bean pulp powder is used as a carrier to prepare a bioactive protein material. The method of the invention performs process improvement based on the enzymolysis of the primary exogenous proteinase preparation, solves the difficult problems of high production cost, unstable exogenous enzyme activity and non-thorough enzymolysis in the endogenous enzymolysis process, and is more suitable for industrialized production; and the prepared bioactive protein powder has the sense, flavor and cultivation effects superior to those of the fish powder, and is a good fish powder substitute.

The invention relates to a production process for preparing biological active small peptide with endogenous enzyme, which is characterized in that low value freshwater fish or aquatic product processing leftover is selected as raw material; the raw material is pulped after being cleaned, and water is added to prepare fish cream; the fish cream is put into a zymohydrolysis device, calcium chloride is added in, and the fish cream is heated to raise and control the temperature to be 53 DEG C to 57 DEG C, the ph value is regulated, zymohydrolysis is conducted, to obtain enzymolysis product; enzyme killing and sterilization are conducted to the enzymolysis product, impurities in enzyme- hydrolyzed solution are filtered, to obtain coarsely filtered enzyme- hydrolyzed solution; the enzyme- hydrolyzed solution is concentrated, to obtain liquid or paste biological active small peptide. The production process pursues the best autologous enzymolysis conditions with the endogenous enzyme system in the mechanism of low value freshwater fish, not only greatly saves the cost, but also simplifies the production process of the biological active small peptide, so that the mass production is easy to be realized.

Belonging to the malt manufacturing field, the invention provides a preparation method of burnt malt with high caramel aroma and beer. The malt preparation method includes the steps of: barley selection, raw material pretreatment, wheat dipping, germination, green malt saccharification, baking and root removal. Specifically, during barley selection, barley with moderate protein content is adoptedas the raw material; the wheat dipping process provides barley grains sufficient internal moisture content to fully stimulate the vitality of the endogenous enzyme system of malt, thus providing sufficient conditions for subsequent acquisition of a Maillard reaction substrate; the saccharification process is carried out in four stages, so that green malt can effectively degrade cellulose, proteinand starch under an appropriate water condition, and the concentration of the Maillard reaction substrate is increased; and then baking is carried out at an appropriate generation temperature of DMHFso as to enrich DMHF product substantially. The obtained malt has significant caramel aroma, the aroma is pure and strong, sensory evaluation indicates that the malt is free of scorched flavor, burntflavor and other foreign and miscellaneous smell, and can be used for production of beer with high caramel aroma.

The invention discloses a method for processing instant flavor fish bean curd with high fish protein preserved at normal temperature. The method comprises the following steps: taking frozen minced fillet as a main raw material; chopping, mixing, molding, cooling, slicing, pickling, packaging and sterilizing to prepare the instant flavor fish bean curd preserved at normal temperature. The texture taste of the sterilized minced fillet product is improved by integrating a bio-enzyme crosslinking technology, an inhibiting effect of the plasma protein on an endogenous enzyme, a polysaccharide structure modifying technology and a variable temperature sterilization technology, long-term preservation of the product under a normal-temperature condition is achieved, and the product is convenient to eat and carry. Meanwhile, the fish protein content in the fish bean curd product is significantly improved by integrated application of the technologies, the product has high nutrient value and good flavor taste, is broad in market prospect, and the consumer demands of consumers on convenience, daintiness, nutrition and safety of the food are met.

The invention discloses a sea cucumber egg nutriment and a preparation method thereof. The sea cucumber egg nutriment is white or light yellow hydroscopic powder free of odor and taste and highly soluble to water and ethanol. The preparation method comprises sea cucumber egg and intestine breaking and enzymolysis, metal ion removal through ion exchange, and freeze drying, wherein the enzymolysis employs conjugated enzymes of exogenous enzymes and endogenous enzymes or single exogenous enzymes; single exogenous enzyme enzymolysis refers to the enzymolysis using single pepsinase or using the mixture of trypsinase, dispase and alkaline proteinase. The invention not only makes wastes in sea cucumber processing with traditional methods into an invigorant rich in nutrition but also reduces pollution of the wastes to the environment. The processing method is simple and reasonable and can be industrialized easily.

The invention discloses an industrialized production method for instant brown rice. The method comprises the following steps of: firstly, carrying out the operation steps of drying, cleaning, grading, stone removing, rice ridging and husked rice separating on unhusked rice as the raw material so as to obtain the original brown rice; and secondly, carrying out soaking, enzymolyzing, curing, dryingand other processing means on the original brown rice to obtain the instant brown rice. In the invention, the common brown rice is used as the raw material to prepare the products by means of an endogenous enzyme germination technology, an exogenous enzyme hydrolysis technology, a brown rice pregelatinization technology, a rapid drying technology and the like; and the prepared products have the advantages of good palatability, short cooking time, long shelf life and the like. By adoption of the industrialized production method disclosed by the invention, the Alpha degree of the prepared brownrice can reach 100%; the cooking time of the brown rice is shorter than 15 minutes and the shelf life of the brown rice is more than 6 months; and the taste of the brown rice is favorably improved.

The invention discloses a biologic modifying method of bran for producing whole wheat flour. The biologic modifying method can comprise the following steps of: crushing large bran, performing sterilization treatment, inoculating microzymes and / or lactic acid bacteria for solid state fermentation, and after the fermentation, performing drying treatment. According to the biologic modifying method disclosed by the invention, the sterilization treatment is performed, so that infectious microbes and endogenous enzymes in the bran are in inactivation, the eating safety is improved, and the shelf life is prolonged; the fermentation effects of microorganisms including the microzymes, the lactic acid bacteria and the like are utilized for modifying the bran, so that the effects are obvious, the operation is easy, the safety is high, and besides, through the fermentation, nutrient components in the bran are obviously improved; the fermentation is performed under solid-state conditions, so that water in the later-stage drying process is easy to remove, and reduction of energy consumption is facilitated. In short, through the technology disclosed by the invention, the preparation of the bran with the characteristics of being high in stability, good in nutritional properties, good in processing properties and the like can be realized, the bran can be used for producing high-quality whole wheat flour, and besides, the biologic modifying method is simple in technology, low in cost and suitable for large-scale industrial production.

The invention belongs to the field of pharmacy, and relates to a brain tumor multiple targeting drug delivery system of a stability polypeptide mediated cross-barrier film, which is composed of stability polypeptide DCDX and c(RGDyK) and liposome. The drug delivery system is can resist an endogenous enzyme barrier and mediate cross-blood cerebral barrier (BBB) and blood-brain tumor barrier(BBTB). The experiment result displays that the liposome can delivers an entrapment model medicine to a target tissue, survival stage of the glioma model animal can be prolonged by the entrapment doxorubicin, and the curative effect of the medicine can be obviously increased. The brain tumor multiple targeting drug delivery system has good application prospect on diagnosis and treatment of brain tumor.

The invention discloses a non-thermal processing method for soft fruit and vegetable cans, and belongs to the technical field of can processing. The method comprises the following steps of sorting, classification, washing, removal of peels, kernels and seeds, slicing, crisp keeping, blanching, cooling, canning, blending, vacuum seal, high hydrostatical sterilization and the like, wherein the pressure of the high hydrostatical sterilization is 400 to 600 MPa, and the pressure maintaining time is 3 to 30 min. In the method, the blanching is combined with the high hydrostatical sterilization, so that compared with the conventional processing technology for the fruit and vegetable cans, the method has the advantages of killing microorganisms, passivating endogenous enzymes, maintaining organoleptic quality such as color, smell, taste and the like and the nutrient components of the product, ensuring food safety, prolonging storage period under the condition of not adding preservatives, and eliminating the cooking smell of the conventional fruit and vegetable cans; and metal cans and glass bottles are replaced by EVOH / PVPP bottles or bags with high oxygen barrier property, so that the soft fruit and vegetable cans are light and portable, attractive, convenient to open and easy to transport.

The invention discloses a production method of deodorized silver carp protein powder, which comprises the steps of taking a low-value freshwater fish, namely a silver carp, as a raw material, preparing surimi pulp, degreasing and deodorizing with saccharomycete fermentation liquid, extracting by an acid method, condensing, and drying to obtain the silver carp protein powder. According to the method, surimi and the rosin saccharomycete fermentation liquid are incubated together, saccharomycete fermentation and active ingredients in the fermentation liquid are used for deodorization, and hydrolysis of an endogenous histenzyme of fish is used to promote protein dissolution. With the adoption of the ultrasonic aided acid method for extraction, the protein extraction rate of the silver carp is increased significantly, and acetic acid can be utilized to deodorize a protein extract effectively. Endogenous enzyme hydrolysis and yeast fermentation liquid deodorization as well as acid method extraction and acetic acid deodorization are performed simultaneously during the whole production process, a bio-enzyme is not required to be added, the technological operation is simple, the cost is low, and the protein powder product is green, safe and good in water solubility.

A shotgun metabolomics approach using MALDI-tandem mass spectrometry was developed for the rapid analysis of cellular metabolites. Through the use of neutral organic solvents to inactivate endogenous enzyme activities (i.e., methanol / chloroform / H2O extraction), multiplexed extraction conditions and combinatorial alterations in matrix stereoelectronic composition and analyte interactions, multiple suites of metabolites were directly ionized and quantitated directly from biologic extracts without the need for prior chromatographic separation. Through combinatorial alterations in 9-aminoacridine charge, aromaticity and stacking, a set of multiplexed conditions was developed that allowed identification of many hundreds of peaks corresponding to metabolites from mouse heart extracts. Identification of metabolite peaks was based on mass accuracy and isomeric species were assigned based on diagnostic fragment ions present during tandem mass spectrometry for many of the identified metabolite peaks.

The invention relates to a Hi-C high-throughput sequencing library construction method applicable to plants. The method comprises the following steps: (1) performing vacuum formaldehyde cross-linkingon sample ice; (2) cracking the cross-linked material, and releasing cell chromatin; performing endogenous enzyme inactivation on the cell chromatin by SDS (Sodium Dodecyl Sulfate), performing enzymedigestion cutting by MboI endonuclease to obtain a material subjected to enzyme digestion, wherein the concentration of the MboI endonuclease is 5000 units / mL, and the enzyme digestion condition is that the material is incubated overnight at 37 DEG C at 900 revolutions per minute; (3) performing end repair on the material subjected to enzyme digestion; (4) performing DNA intramolecular ligation after end repair; (5) decrosslinking the material subjected to intramolecular ligation, removing the non-ligation terminal biotin to obtain purified DNA, randomly breaking the DNA into fragments favorable for sequencing by ultrasonic waves, so as to obtain the DNA sequencing library. According to the method, by designing the appropriate formaldehyde cross-linking conditions, endogenous enzyme inactivation conditions and appropriate tool enzymes, the technical problems that the cytoplasm is difficultly released from plant cells and the library construction effect of low-quality plant samples is poor are solved, the Hi-C high-throughput sequencing library construction of the plants is realized, and the application range of the Hi-C technology is widened.

The present disclosure provides an endogenous enzyme-triggered DNA walker and its application for detecting UDG, The DNA walker comprises gold nanoparticles, at least one walking chain and a pluralityof track chains, wherein the walking chain and the tracks are single-stranded DNA, one end of the walking chain is connected with the gold nanoparticles, one end of each track chain is connected to the gold nanoparticles, the other end of each track chain is connected to fluorophore, the walking chain contains a first sequence, the track chain contains a second sequence, the first sequence is complementary to the second sequence, and one base in the second sequence is a uracil base. The constructed DNA walker is capable of sensitive imaging of UDG activity in cells.

The invention relates to a method for improving boiling property and palatability of germinated brown rice, which belongs to the technical field of food machining. The method specifically comprises treating brown rice by using ultrasonic auxiliary external cellulose compound pectinase, and through the synergistic effect of endogenous enzyme activated when the brown rice sprouts, the brown rice cortex is subjected to partial hydrolysis, so as to make the germinated brown rice cortex loose and porous. The germinated brown rice produced by using the method is good in boiling property, light in bran wax taste and good in palatability, and the hardness of the germinated brown rice after being boiled is reduced by 10-30% when being compared with the hardness of the untreated germinated brown rice.

The invention discloses a making method of high-density gamma-aminobutyric acid powder, which comprises the following steps: adopting rice bran as raw material; adding glutamic acid, calcium chloride and phosphopyridoxal to obtain the reacting liquid with rich gamma-aminobutyric acid through endogenous enzyme; centrifuging and separating to obtain supernatant; adding alcohol to remove impurity; hyperfiltering; condensing; drying to obtain the product with content over 50%.

Provided herein are methods for imaging an optically labeled target cell implanted in vivo using a reporter construct that encodes an enzyme that is not endogenous to the target cell. The disclosed methods include the steps of: (a) introducing target cell transformed with non-endogenous enzyme into a mammalian subject; (b) contacting the transformed target cell with a cell-permeable soluble fluorescent dye; and (c) observing a fluorescent signal generated by the target cell present in the mammalian subject. In some embodiments, the non-endogenous enzyme comprises a nitroreductase. In some embodiments, the methods may also include the step of tracking the fluorescent target cells in the mammalian subject over time.

The present invention is related to biosynthetic oil compositions and methods of making thereof. In some embodiments, the invention relates to the use of endogenous enzymes in plants capable of synthesizing oil. In preferred embodiments, said plants are algae. In further embodiments, said algae are from the family Chlamydomonas, Nannochloropsis, Dunaliella, Chlorella and Scenedesmus. In still further embodiments, said endogenous enzymes are diacylglycerol acyltransferases.

The invention discloses a method for constructing a high-yield sclareol engineering strain by a synthetic biology methdo, and an application thereof. The construction method for the strain relates to express plant sourced key enzymes for synthesizing sclareol, wherein the key enzymes comprise a pyrophosphoric acid ladanum enediol ester synthase and a sclareol synthase; and overexpress a combination of one or more yeast endogenous enzyme-based genes, wherein the enzyme-based genes comprise a key enzyme of a mevalonic acid approach-(hydroxymethyl) coenzyme A reductase, and two enzymes of an isopentenylpyrophosphate approach-a farnesyl pyrophosphate synthase and a geranylgeranyl diphosphate synthase. The method can efficiently produce sclareol, with a shake flask fermentation level reaches 9 mg / l, and has industrial application prospects.

The invention relates to a production method of music germinated brown rice rich in selenium and high r-aminobutyric acid and a product thereof, and belongs to the technical filed of rice deep and finishing processing. High-quality fresh rice produced in a green food base during that time is selected as a raw material, and full and robust brown rice can be obtained by hulling, husked rice separation and selection. The brown rice is processed by magnetic separation, high voltage electrostatic field and ozone sterilization, and then is put in a music germination pot, and a red nano-selenium nutrient solution with appropriate concentration is added and oxygen is pumped to soak; the temperature control and music frequency vibration activate the physiological activities of protease, dehydrogenase and other endogenous enzymes of the brown rice to promote the germination. The product obtained after processing is rich in selenium of 1.5mg / kg and high r-aminobutyric acid of 280mg / 100g, and has rich nutrition, good taste, can meet the requirements of health of human body. Compared with the prior art of currently used chemical substances, microorganism fermentation method and the like, the physical germination method is natural and environment-friendly, efficient, nutritious, fashionable, and low in production cost.

The invention discloses a method for comprehensively extracting active matters from rice processing byproducts such as rice bran and grain embryo. The rice bran, the grain embryo and the like are taken as raw materials, and the method comprises the following steps of: converting by using an endogenous enzyme method and extracting to obtain gamma-aminobutyric acid; extracting the active matters according to physical and chemical characteristics of the active matters in a certain order; extracting natural rice bran ceramide from a filter cake subjected to gamma-aminobutyric acid extraction by using an amphiprotic solvent; stepwise precipitating by using ethanol to obtain rice bran polysaccharide; and extruding and bulking the filter cake to prepare rice bran dietary fibers. The rice processing byproduct resources such as the rice bran and the grain embryo are fully utilized, the wastes generated in the production process are reduced, and higher economic and social benefits are achieved.

The invention provides a production method of Hubei flavored fermented mandarin fish. The production method comprises the following steps: pretreatment, bacteria-reducing treatment, composite fishy smell removal, pickling, gradient temperature-controlled humidity-controlled fermentation, bittern washing, vacuum sealing, fast freezing and the like. The bacteria-reducing treatment is adopted to effectively reduce the initial bacterial colonies on the fish body surface. The composite fishy smell removal is adopted to treat the fish body, thereby effectively lowering the fishy smell. The wet pickling treatment is adopted to effectively isolate oxygen, thereby decelerating deterioration of the fish, and enabling the salt and spices to uniformly penetrate into the fish tissues. The gradient temperature-controlled humidity-controlled fermentation is adopted, and the microbes and endogenous enzyme are used for fermenting the fish under appropriate conditions to generate the special flavor substance.

The invention relates to a preparation method of peeled black garlic. Endogenous enzymes of immersed peeled garlic cloves are activated mainly by controlling temperature and humidity in a fermentation room, so as to finish a natural fermentation process of the inside of garlic in the fermentation room. The peeled black garlic prepared by the method preserves and increases physiological activity of the garlic maximally, allowing the black garlic to have good taste without irritating spicy taste.

The invention discloses a method for preparing Noni juice. The method includes the steps: 1) cleaning picked 7-8% ripe normal Noni fruits, dipping the Noni juice in chlorine dioxide water solution, washing the Noni fruits again, drying the Noni fruits, hermetically preserving the fruits and after-ripening the fruits; 2) pressing and separating the fruits, adding normal juice of passion fruits and mango puree into the fruits and mixing the fruits, the normal juice and the mango puree; and 3) homogenizing, filling and sterilizing the mixture to obtain the Noni juice. Chlorine dioxide is used for sterilizing, the Noni fruits are after-ripened at a certain sealing storage temperature, aril breakdown is promoted by endogenous enzyme in the Noni fruits, the juice can be extracted, flavor is improved by adding a small amount of normal juice of the passion fruits and banana puree, the prepared Noni juice beverage is sour, sweet and delicious, the flavor is coordinated, and original flavor and nutrition of the Noni fruits are kept.

The invention relates to a method for capturing interacted DNA fragments in tissue nuclear genome. The method comprises the following steps: S1) taking fresh animal tissue, fragmenting the tissue and filtering the tissue with a cell sieve to obtain a filtrate containing dispersed tissue cell, performing centrifugation to collect the tissue cell; S2) resuspending the obtained tissue cell obtained in the S1 in formaldehyde for crosslinking; S3) cracking the crosslinked tissue cell; S4) inactivating endogenous enzyme, adding restrictive enzyme digesting genome; S5) filling a cohesive end generated through restrictive enzyme digesting genome in the S4, adding a marker; S6) connecting the DNA fragments which are mutually closed due to combination with protein; and S7) fragmenting chromatin to capture molecule-labelled fragments, and constructing a library. According to the invention, the animal tissue is subjected to Hi-C operation, a usage scope of a Hi-C technology is enlarged, the Hi-C technology is not limited in cell line, and more species tissue samples can be used for genomics research.