108 results about "Endogenous enzymes" patented technology

The invention discloses cured beef and a preparation method thereof, and belongs to the technical field of fermented food. Comprising the following steps: trimming and dicing beef, adding seasoning spices, and adding a microbial agent for fermentation, the microbial agent is prepared from lactobacillus plantarum NR1-7 and aroma-producing yeast. According to the invention, the cured beef fermented by adding the Chinese prickly ash extracting solution, the lactobacillus plantarum NR1-7 and the aroma-producing yeast is relatively high in viable count, good in color and luster, rich in free amino acid quantity, moderate in protease activity and appropriate in organic acid content, and the pH value is stabilized to be about 5.5. Compared with natural fermentation, the growth and reproduction of inoculated dominant bacteria can accelerate the degradation of beef components and generate rich flavor, the pepper extract can ensure the complete beef structure by weakening the activity of endogenous enzyme, and in addition, the sour taste can be alleviated, the excessive degradation of fat can be relieved, and the fermentation quality of the cured beef can be improved.

The invention belongs to the field of microbiological detection, and discloses a rapid detection chromogenic culture medium for pseudomonas, and the chromogenic detection culture medium comprises a chromogenic substrate, an endogenous enzyme promoter, a basic culture medium and a bacteriostatic agent. Specifically, the pseudomonas can react with a chromogenic substrate, so that a bacterial colony is blue-green, and other non-pseudomonas does not grow or weakly grow under the action of a bacteriostatic agent, or the chromogenic substrate different from the pseudomonas is utilized to display purple red or other colors which are inconsistent with the color of the pseudomonas bacteria, so that the pseudomonas bacteria can be distinguished, and the pseudomonas bacteria can be distinguished. And rapid separation and identification are realized. According to the rapid detection chromogenic culture medium for the pseudomonas, the glycoside chromogenic substrate and the endogenous enzyme accelerant are utilized, the problem that when the rapid detection chromogenic culture medium for the pseudomonas is prepared, the pseudomonas has chromatic aberration or does not have color development is solved, and the blank of the rapid detection chromogenic culture medium for the pseudomonas in the domestic market is filled.

The invention relates to the technical field of food processing, and particularly discloses soybean flavor peptide and a preparation method and application thereof. Aspergillus oryzae is selected as a leavening agent, a microbial endogenous enzyme generated by full fermentation of the aspergillus oryzae is utilized to cooperate with a compound enzyme preparation to perform combined action, abundant enzyme systems effectively perform enzymolysis on macromolecules such as protein, and related taste substances are provided for subsequent enzymolysis products; in the soybean flavor peptide prepared by enzyme hydrolysis and Maillard reaction, the contents of target peptide and target amino acid (umami peptide, umami amino acid and the like) are high; when the soybean flavor peptide is applied to the compound seasoning with the main function of increasing freshness, the freshness score can be increased by 0.5-2 times. In addition, on the basis of improving freshness, the taste richness of the food or the compound seasoning can be enhanced, and the aftertaste time is prolonged.

The invention provides a ganoderma lucidum fermentation lysate as well as a preparation method and application thereof in preparation of cosmetics. The preparation method of the ganoderma lucidum fermentation lysate comprises the steps of induced fermentation, catalytic degradation, membrane filtration, spray drying and the like. By inducing endogenous enzyme and metal ion regulation catalysis, the ganoderma lucidum fermentation lysate can be prepared in an environment-friendly, economical and efficient manner, the content of ganoderma lucidum polysaccharide in the prepared ganoderma lucidum fermentation lysate reaches 18% or above, and the content of beta-glucan in ganoderma lucidum polysaccharide is larger than 5%. Experiments prove that the ganoderma lucidum fermentation lysate has good antioxidant activity and an anti-aging effect, and has a wide market application prospect in the field of anti-aging cosmetics.

The invention relates to a cow's milk endogenous antibacterial peptide and application thereof, the cow's milk endogenous antibacterial peptide is an antibacterial peptide compound PKTKVIPYVRYL from endogenous enzyme hydrolyzed bovine casein, and the amino acid sequence of the antibacterial peptide compound is Pro-Lys-Thr-Lys-Val-Ile-Pro-Tyr-Val-Arg-Tyr-Leu. The compound has wide inhibitory activity on gram-positive bacteria and gram-negative bacteria, including porphyromonas gingivalis, escherichia coli, staphylococcus aureus and the like, respectively. The antibacterial peptide can be used as a health care product and / or a medical instrument or a medicine for preventing and / or reducing infectious diseases, a food bacteriostatic agent, novel functional food, pet food, an animal feed additive and daily chemicals and has a good application prospect.

The invention discloses a method for converting rutin into quercetin through biological catalysis, and belongs to the technical field of biological catalysis. The method comprises the following steps: mixing rutin and a solvent to obtain a quasi-solid rutin suspension; mixing the paraphlonia plant endogenous enzyme and the quasi-solid rutin suspension for enzymolysis, and then filtering, washing and drying to obtain quercetin; the solvent is methanol with the concentration of 5-50%, ethanol with the concentration of 5-50% or acetone with the concentration of 10-50%. The method is mild in biological catalytic reaction condition, does not generate pollutants such as acid waste liquid, and meets the environmental protection requirement. Efficient conversion of quercetin is realized by adopting a quasi-solid rutin strategy (rutin suspension reaction system), and the problem of substrate solubility limitation is solved. High-purity quercetin can be obtained through simple washing, the product purity is larger than 95%, and food and clinical application can be met.

The invention discloses a fresh plant directed biotransformation method based on endogenous enzyme activation and a product and application thereof. The core of the preparation method is as follows: fresh plants (tuberous roots, stems or fruits) which are harvested in a ripe state and have metabolism in a dormant state (the respiration rate is lower than 5 mg CO / kg.h) are taken as raw materials, the raw materials are treated in an environment with the specific pressure being 0.02-0.20 MPa, the temperature being 70-121 DEG C and the humidity being 50-95%, a specific impregnating compound can be selectively combined, and the raw materials can be prepared into the plant culture medium. Under the conditions that exogenous microorganisms are not introduced and plant tissue structures are not damaged, the catalytic function of an endogenous metabolic enzyme system is safely and efficiently restarted and maximally promoted, and components of a plant body are driven to realize directional biotransformation. According to the process, conversion of primary metabolites and / or secondary metabolites is remarkably promoted, the content conversion of one or more beneficial components in the primary metabolites and / or the secondary metabolites is improved, and meanwhile, the biological and pharmaceutical structures and inherent metabonomics characteristics of the plants are completely reserved. The biotransformation product can be used as a high-quality raw material to be applied to medicines, common foods, health foods, special diet foods, daily chemical products and disinfection products, and can be further prepared into traditional Chinese medicine decoction pieces, solid preparations, semi-solid preparations and liquid preparations by adding or not adding pharmaceutically acceptable auxiliary materials.

A mutant lysophospholipase is provided. A gene encoding the lysophospholipase, as well as a vector and a host cell comprising the gene, are further provided. Furthermore, a use of the lysophospholipase is further provided. The present invention also provides a mutant Aspergillus niger strain and a use thereof. In particular, the present invention provides a mutant Aspergillus niger strain that is an orotate phosphoribosyltransferase auxotroph and has improved ability to produce an endogenous enzyme, preferably a lysophospholipase, compared to a non-mutant strain. The present invention further provides a use of the strain. For example, the strain can be used to express the mutant lysophospholipase of the present invention.

The invention discloses a method for preparing rare saponin by utilizing panax japonicus endogenous enzyme, and relates to the technical field of extraction, the panax japonicus plant material or crude extract thereof is directly taken as a starting point, the enzyme system is simple and convenient to obtain (fresh medicinal material low-temperature homogenate extraction or direct use of freeze-dried powder), the reaction system is simple, and the operation is easy. According to the preparation process provided by the invention, the catalytic action of endogenous enzyme is fully utilized, so that the introduction of exogenous substances (acid-base, protein and the like) in the extract is completely avoided; the method disclosed by the invention is high in catalytic efficiency, can quickly realize complete conversion of saponin (within 10min) under specific conditions, and has extremely high industrial application.

The invention discloses an immunostaining reagent for MHC-II expression detection of a gastric cancer biopsy sample, and belongs to the field of bioengineering. The invention establishes a preparation method of an immunohistochemical staining section. The preparation method specifically comprises the following steps: step 1, early-stage treatment: baking a tissue section, dewaxing, rehydrating, subsequently adding an antigen solution for repairing, adding an endogenous enzyme inhibitor for incubation, adding a sealing agent for sealing and the like; step 2, antibody incubation: adding a first antibody and a second antibody for incubation; and step 3, post-treatment: dyeing, re-dyeing, bluing, sealing and the like. A mixed buffer solution is used as an endogenous enzyme inhibitor, so that the background staining intensity is effectively reduced, and the staining sensitivity and specificity are improved; meanwhile, a staining result interpretation standard is established, it is proved that MHC-II expression is remarkably related to pathological remission of the gastric cancer patient, and an evaluation method is provided for pathological remission of the gastric cancer patient.

This invention relates to a recombinant Halomonas strain that produces polyhydroxyalkanoates (PHA) by overexpressing endogenous enzymes encoding ADP-dependent acetyl-CoA synthase (ADP-ACS). acd This gene not only enhances the recombinant bacterium's tolerance to acetic acid but also enables it to efficiently convert acetic acid or acetate substrates into acetyl-CoA, thereby participating in the tricarboxylic acid cycle (TCA cycle) and the synthesis of PHA. The present invention also relates to a method for producing PHA using the aforementioned recombinant Halomonas bacterium employing a pH-stat strategy.

The invention discloses an enzyme-activated fluorescent molecular precursor as well as a preparation method and application thereof. The enzyme-activated fluorescent molecular precursor has a chemical structure as shown in a formula I which is described in the specification. The invention develops an enzyme-activated fluorescent molecular precursor which can be used for real-time imaging of tumors by targeting exogenous enzyme to the tumors and specifically activating a probe at the tumors; according to the invention, the exogenous enzyme is introduced as an activation trigger, and normal cells do not have the exogenous enzyme, so that the problem of co-expression of the endogenous enzyme in non-tumor tissues can be avoided, background fluorescence signals can be reduced to the greatest extent, and the imaging specificity and accuracy are remarkably improved.

In the process of producing indigo naturalis by taking strobilanthes cusia as a raw material, a very unpleasant smell is generated, a large amount of alkaline wastewater is discharged, and serious environmental pollution is caused. Therefore, development of a more environment-friendly production process and a higher value-added strobilanthes cusia extract is extremely urgent. The invention innovatively provides a green and efficient production process, namely integrally producing the indigo strobilanthes extract and the phenolic acid extract with higher additional values through endogenous enzymatic conversion coupled membrane separation and freeze drying technologies. Through an endogenous enzyme conversion process, the indigo extract with indigo content 20 times and indirubin content 1.8 times higher than that of traditional indigo can be prepared within one day. The phenolic acid extract is recovered from the indigo extract production wastewater by adopting membrane separation and freeze drying processes, and meanwhile, zero discharge of the wastewater can be realized. The prepared indigo extract shows a more excellent dyeing effect on pure cotton poplin than an Indian natural indigo extract. In addition, the prepared phenolic acid extract shows excellent inhibitory activity (IC50 = 32 [mu] g / mL) on staphylococcus aureus, and the wound healing rate of mice exceeds 98%. The method lays a foundation for high-valued and comprehensive utilization of strobilanthes cusia resources, and is of great significance to extension and sustainable and healthy development of a strobilanthes cusia industrial chain.

The invention belongs to the technical field of animal breeding, and discloses an application of N-methyltyramine hydrochloride in a breeding animal feed and the feed, the N-methyltyramine hydrochloride is added into the breeding animal feed in a mixed or mixed granulation form, and preferably, 45g of N-methyltyramine hydrochloride is added into each ton of compound feed. The N-methyltyramine hydrochloride is chemically synthesized by taking an effective stomach-invigorating component N-methyltyramine in the traditional Chinese herbal medicine immature bitter orange as a technical basis. When the N-methyltyramine hydrochloride is added into the feed for the bred animals in a proper proportion, the secretion of endogenous enzyme of the bred animals can be promoted, so that the feed reward of the bred animals is improved.

The invention provides an antheraea pernyi fresh-keeping method which comprises the following steps: S1, dividing a first group and a second group with different freezing response characteristics according to development biological characteristics of antheraea pernyi; the freezing response characteristic is represented by at least one parameter of tissue moisture content, methylene blue penetration depth and body wall elasticity modulus; s2, differential preprocessing is applied to the first group and the second group, so that the difference of the first group and the second group on any characterization parameter does not exceed a preset threshold value; s3, combining the two groups of tussah and freezing; in the first cooling stage, cooling from 0-1 DEG C to-10 DEG C at the speed of 0.8-1.2 DEG C / min; in the second cooling stage, cooling is conducted from-10 DEG C to-30 DEG C at the speed of 0.45-0.55 DEG C / min; in the third cooling stage, cooling from-30 DEG C to-45 DEG C at the speed of 1.8-2 DEG C / min; and S4, after freezing is completed, vacuum packaging is performed, and storage is performed at minus 45-minus 40 DEG C. According to the fresh-keeping method for the tussah, the integrity of a cell structure is maintained, juice loss is reduced, and abnormal release of endogenous enzyme is inhibited, so that nutritional ingredients are better reserved in the long-term freezing storage process, and the sensory characteristics of compact meat, uniform color and luster and no peculiar smell are maintained.

The invention relates to a method of producing an ester of a fatty acid and a C1-C4 alcohol, comprising successive steps of: cultivating cells of a microalga strain on a growth medium, harvesting the cultivated cells and concentrating them to obtain a wet cell pellet, suspending said wet cell pellet in an hydroalcoholic solution comprising said C1-C4 alcohol, incubating the cell suspension thus formed so as to form said ester of a fatty acid and a C1-C4 alcohol by transesterification and / or esterification of lipids produced by the microalga cells catalyzed by endogenous enzymes of said cells, and recovering said ester of a fatty acid and a C1-C4 alcohol from said cell suspension. The growth medium can advantageously be a dark fermentation effluent.

The invention relates to a sugar-controlling and weight-losing functional food cooperatively conditioned by endogenous enzymes of processing raw materials and a preparation method thereof, and belongs to the technical field of food. The raw materials comprise 6-10 parts of white kidney beans, 4-8 parts of lucid ganoderma, 20-40 parts of black-skin and green-kernel black beans, 3-6 parts of mung beans and 40-60 parts of wheat flour / rice flour. A dual biological activation process is adopted: white kidney beans are subjected to low-temperature enzymolysis by yeast powder and probiotic soybean enzymes (1%, 25-35 DEG C) for 2-4 hours to release an alpha-amylase inhibitor, and ganoderma lucidum is subjected to weak-base hydrolysis by sodium bicarbonate (1-3%) for 2-4 hours to activate a triterpenoid glycosidase inhibitor; and performing compound fermentation on the zymolyte and the alkaline hydrolysis product for 2-4 hours, and then adding a cereal base material to activate the endogenous enzyme activity. High fiber combination (more than 15%) is used for prolonging satiety and reducing fat absorption, resistant starch (heat is only 50% of saccharides) is used for inhibiting lipid synthesis, and quadruple synergy (enzyme inhibition is more than 90% / resistant starch / low GI / high fiber) is combined, so that dual effects of postprandial blood sugar reduction and weight control are realized.

The invention discloses a preparation method and application of a composite plant extract for inhibiting glutamate dehydrogenase activity, and belongs to the technical field of plant extract preparation. The process provided by the invention comprises the following steps: mixing and crushing licorice roots and honeysuckle buds together, adding water, adding cellulase and pectinase, carrying out ultrasonic-assisted enzymolysis to release cell contents, carrying out transglycosylation reaction by using endogenous enzymes in the cell contents, drying the obtained dissolved substance, carrying out alcohol extraction, centrifuging, and taking filtrate; and freeze-drying to obtain the composite plant extract. The extract has a good inhibition effect on the enzymatic activity of glutamate dehydrogenase, and can not only effectively reduce the odor degree of ammonia in excrement but also improve the digestive ability of pet cats after the pet cats are fed with the extract.

The invention provides a pea protein high-valued preparation method and system based on molecular level structure regulation and enzymolysis targeting synergy, and belongs to the field of vegetable protein functional processing. Pure natural pea meal is taken as a raw material, a porous structure is directionally generated through a superheated steam instantaneous phase change blasting mechanism, and phytic acid inactivation and endogenous enzyme retention are synchronously realized; by combining alpha-amylase to precut starch, alpha-galactosidase to degrade oligosaccharide in a targeted manner and a double-enzyme gradient time sequence activation mechanism, the enzymolysis competitive inhibition problem is solved; and finally, the protein dissolution index and digestibility are improved through microporous nanofiber compounding and a multi-scale structure interaction mechanism, and a steam-enzymolysis collaborative module is integrated in the system, so that an innovative solution is provided for natural plant protein processing. The obtained thin bean flour has breakthrough integration of nutritional advantages of pea protein and natural viscoelasticity regulation and control characteristics, has cold and hot double-soluble quick-acting property, low sensitization and long-term preservation property, and can be expanded to the field of precise nutritional food development.

The present application relates to a freshness indicator protein related to quality traits of low-temperature stored Siniperca chuatsi, endogenous proteases and applications. The freshness indicator endogenous proteases are related to postmortem proteolysis and meat softening of Siniperca chuatsi, including cathepsin B, cathepsin L and calpain. The freshness indicator proteins are related to quality changes occurring during postmortem cold storage of Siniperca chuatsi, including myoactin 1, myosin binding protein, LDB protein, actin-related protein, etc. The present application determines the change rule of endogenous protease activity of Siniperca chuatsi at different storage stages, and the dynamic influence on muscle proteolysis and muscle degradation of Siniperca chuatsi, and reveals the internal relationship with fish meat sensory quality and processing characteristics, and clearly defines the "critical point" of quality deterioration of Siniperca chuatsi. It provides clear guidance for the control of endogenous enzyme-mediated quality deterioration in the processing and storage process of Siniperca chuatsi.

The invention belongs to the technical field of food processing, and particularly relates to a garlic pickling method capable of promoting nutrient absorption, which comprises the following steps: S1, performing micropore puncture pretreatment on carefully selected complete garlic bulbs to form through pore channels in specific directions so as to establish rapid permeation channels of subsequent treatment substances; s2, sequentially immersing the garlic bulbs punctured by the micropores into a low-concentration and low-temperature first ethanol solution and a high-concentration and high-temperature second ethanol solution for gradient ethanol dehydration, then carrying out heat treatment to remove part of water, carrying out primary sterilization and activating the endogenous enzyme activity of the garlic bodies. According to the invention, physical pretreatment, gradient dehydration, heat treatment activation and compounding of absorption-promoting pickling liquid are combined with a dynamic alternate pickling process to synergistically destroy garlic cell barriers, activate endogenous enzyme conversion and introduce absorption-promoting functional components, so that the pickled garlic product with good flavor and nutrition easier to absorb by human bodies is prepared within a short time.

This invention relates to the field of grain processing technology, and in particular to a method for improving the nutritional value of brown rice and reducing its glycemic index. The invention uses brown rice as raw material, processing it through a process of mist-wetting for nutrient enrichment, integrated microwave gelatinization and drying, tempering, and milling to produce semi-cooked rice (parboiled rice). The brown rice processed by the method provided by this invention is clean and odorless, slows down the rate of water penetration, prevents grain cracking, reduces the rate of broken rice during subsequent milling, and, under the action of endogenous enzymes, enriches nutrients such as GABA, flavonoids, and free amino acids. Furthermore, the bran layer of the dried brown rice is easily separated.

This invention relates to the field of feed additive technology, specifically to a feed additive that promotes digestion and absorption and its preparation process. The preparation process of a feed additive that promotes digestion and absorption includes: preparing antioxidant-modified chicken fat; preparing an extract; preparing a composite oil phase; and preparing the feed additive. In this invention, the extract of Shenqu (a type of fermented wheat) can directly compensate for insufficient endogenous enzyme secretion in laying hens, rapidly decomposing starch in feed and chicken fat in plant proteins, laying the foundation for subsequent absorption. The extract of Zhishi (a type of immature bitter orange) accelerates gastrointestinal motility and digesta emptying, while also promoting bile and pancreatic juice secretion, further improving the digestibility and utilization rate of fat-soluble components in laying hens. The extract of pine pollen can repair the intestinal mucosa, increase intestinal villus height, increase the nutrient absorption area, and regulate the intestinal microecology, reducing nutrient loss and intestinal stress. The combined use of these three ingredients synergistically promotes the digestive and absorptive capacity of laying hens.

The invention discloses a directional preparation method of a coprinus comatus protein peptide Maillard reaction product with salt reducing and saltiness increasing capabilities. The directional preparation method comprises the following steps: S1, pretreatment; s2, raw material endogenous enzyme inactivation; s3, first-step enzymolysis: adding cellulase for enzymolysis; s4, performing enzyme deactivation treatment; s5, second-step enzymolysis: adding flavourzyme and aminopeptidase for enzymolysis; s6, performing enzyme deactivation treatment; s7, preparing supernate of the enzymatic hydrolysate; s8, preparation of a Maillard reaction product: adding D-xylose and L-cysteine to carry out a Maillard thermal reaction, and obtaining the coprinus comatus protein peptide Maillard reaction product after the reaction; wherein the addition amount of the D-xylose is 10-12% of the solid content of the enzymatic hydrolysate, and the addition amount of the L-cysteine is 6-13% of the solid content of the enzymatic hydrolysate. According to the method, cellulase, flavourzyme and aminopeptidase are selected to perform two-step hydrolysis on the coprinus comatus, then the Maillard reaction is performed, the ratio of D-xylose to L-cysteine and the reaction time are regulated and controlled, freshness and saltiness are increased to the maximum extent, and the fragrance or meat fragrance of the coprinus comatus is reserved.

The present invention relates to a cleaning composition, preferably a cleaning composition for use in automatic dish washing and / or laundry washing, which cleaning composition comprises an Alkalitalea saponiacus endogenous enzyme or a variant thereof. The invention also relates to a method of cleaning using the composition of the invention and to the use of an enzyme in a cleaning composition.

This invention discloses a cooking utensil and a method for preparing sleep-enhancing factors enriched therein, belonging to the field of household appliances. The method for preparing sleep-enhancing factors enriched in the cooking utensil includes a stress enzymatic hydrolysis process and a baking process. The stress enzymatic hydrolysis process refers to pre-treating the ingredients to activate their endogenous enzymes. Pre-treatment includes at least one of the following: steam heating and humidification treatment, periodic heating-cooling treatment, low-temperature treatment, low-oxygen treatment, negative pressure treatment, high-pressure treatment, ultrasonic treatment, pulsed light treatment, and plasma treatment. The baking process refers to baking the ingredients treated with the stress enzymatic hydrolysis process. The preparation method of this invention can significantly increase the GABA content of the ingredients, while also improving the bitter taste and enhancing the aroma. In other words, this invention solves the problem that existing cooking methods cannot simultaneously achieve good taste and high nutritional value.

The invention relates to the technical field of bacteriostatic agents, in particular to a radix scutellariae extract with a bacteriostatic effect and a preparation method. The preparation method comprises the following steps: weighing scutellaria baicalensis root powder, putting the scutellaria baicalensis root powder into a polyethylene bag, adding a hydroxypropyl-beta-cyclodextrin aqueous solution into the bag, sealing by a sealing machine, and putting a sample into ultrahigh pressure equipment. The preparation method comprises the following steps: after ultrahigh-pressure extraction is completed, transferring a material into a centrifugal tube, centrifuging to take supernate which is an extracting solution of baicalin and baicalein, heating and stirring the supernate in a water bath kettle, converting the baicalin into the baicalein under the action of endogenous enzyme, and performing vacuum freeze drying after the reaction is completed to obtain the hydroxypropyl-beta-cyclodextrin clathrate extract. According to the invention, baicalin and baicalein in scutellaria baicalensis are simultaneously extracted by adopting an ultrahigh pressure assisted hydroxypropyl-beta-cyclodextrin aqueous solution, and an extract (UP-HP-beta-CD extract) clathrated by hydroxypropyl-beta-cyclodextrin is prepared by cooperating an endogenous enzyme enzymolysis method with a hydroxypropyl-beta-cyclodextrin clathration technology. The extraction efficiency, solubility, biological activity and other properties of effective components in scutellaria baicalensis are improved, the antibacterial effect of the scutellaria baicalensis extract is further improved, and the prepared scutellaria baicalensis antibacterial extract is simple in preparation method, easy and convenient to operate, environmentally friendly, safe, capable of saving energy in process, good in environmental benefit and capable of being applied to industrial large-scale production.

The present invention relates to a method for preparing a broccoli extract and its use in controlling spider mites. The broccoli extract is obtained by enzymatically hydrolyzing broccoli using an endogenous enzyme system, extracting and concentrating the active ingredients in the broccoli using a solvent, and finally drying the broccoli extract. The broccoli extract can be used to prepare acaricides.

The application provides an edible mushroom culture medium prepared from yellow water, a preparation method and application thereof. The preparation method comprises the following steps: subjecting yellow water to a variable-temperature process; adding a compound enzyme to the yellow water subjected to the variable-temperature process for compound enzymolysis treatment; adjusting the pH of the yellow water subjected to the compound enzymolysis treatment to 7.0, collecting the precipitate by centrifugation, and drying to obtain a solid powder; and adding an auxiliary material to the solid powder, and stirring uniformly to obtain the edible mushroom culture medium. The method of the application activates the endogenous enzyme of the bacteria in the yellow water through a variable-temperature process, and then adds a compound enzyme for compound enzymolysis, so that the bacteria in the yellow water are fully lysed, and the bacteria in the yellow water release more efficiently organic nitrogen sources, carbon sources and organic small molecules and other nutrients, the obtained culture medium has more abundant nutrient components, is more suitable for the culture of edible mushrooms, can effectively improve the yield of the cultured edible mushrooms, and shortens the culture period and reduces the production cost.

The invention provides a method for obtaining a garlic fermentation extract with high content of S-allyl-L-cysteine, and belongs to the technical field of biology. The bacillus subtilis capable of producing invertase is separated from a natto product, and the bacillus subtilis can be applied to food and is high in passage stability; the obtained invertase has transglutaminyl ability and thermal stability, the enzyme activity is not reduced due to the influence of conditions in the biosynthesis process, and gamma-glutamyl-S-allyl-L-cysteine (SAC) can be efficiently biotransformed to obtain S-allyl-L-cysteine (SAC) when the invertase is applied to preparation of the garlic extract. Compared with endogenous enzyme self-transformation, the method has the advantages that the time required for obtaining high-content SAC is remarkably shortened, and the SAC content in the garlic fermentation extract can reach 0.8%, is 200 times of the SAC content in fresh garlic, and is far higher than the market product level.