398 results about "Coenzyme A biosynthesis" patented technology

The invention relates to a human mesenchymal stem cell culture medium. According to the culture medium, the basal culture medium comprises the following components based on the final concentration: 1-2g/L of human serum albumin, 5-10mg/L of transferring, 2-8mg/L of fibronectin, 1-4mg/L of laminin, 50g/L of Fe(NO3)3.9H2O, 417g/L of FeSO4.7H2O, 1-3mu g/L of estradiol, 2-5mu g/L of testosterone, 1-3mu g/L of progesterone, 39.25-117.74 mu g/L of dexamethasone, 5-10mg/L of insulin, 376.36mg/L of riboflavin, 80.96-242.87mg/L of coenzyme A, 4.41-6.17mg/L of butanediamine, 1-2mg/L of taurine, 0.61-1.85mg/L of aminoethanol, 8.81-26.42mg/L of pyruvic acid, 3.78-7.56mu g/L of sodium selenate, 292.3-584.6mg/L of L-glutamine, 2-8mu g/L of vascular endothelial growth factor, 4-10mu g/L of epidermal growth factor, 4-10mu g/L of basic fibroblast growth factor, 1-5mu g/L of leukaemia inhibitory factor, 1-5mu g/L of insulin-like growth factor-I and 2-8mu g/L of stem cell factor. The culture medium does not contain the animal serum, the potential animal endogenous endotoxin or virus of the animal serum is eliminated, and the culture medium is conveniently applied to clinics.

The invention describes a process for preparing acetone starting from acetyl-coenzyme A comprising process steps A. enzymatic conversion of acetyl-CoA into acetoacetyl-CoA B. enzymatic conversion of acetoacetyl-CoA into acetoacetate and CoA and C. decarboxylation of acetoacetate to acetone and CO₂, which is characterized in that the coenzyme A is not transferred in process step B to an acceptor molecule. In addition, process step B is surprisingly catalysed by enzymes of the classes of acyl-CoA thioesterase, acyl-CoA synthetase or acyl-CoA thiokinase.

A completely novel metabolic pathway is concerned, because the enzymatic hydrolysis of acetoacetyl-CoA without simultaneous transfer of CoA to a receptor molecule has never previously been described for any microbial enzyme.

Novel 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase inhibitors are useful as antihypercholesterolemic agents and are represented by the following general structural formula (II):

The present invention relates to compounds of the Formula (I), wherein X₁ and X₂ independently represent O, NH or S; R₁ and R₂ are independently selected from the group consisting of a C1-C30 hydrocarbyl group, an amino acid bonded via an amide bond or a peptide bonded via an amide bond each having up to 200 amino acids, the conjugated residue X₁ or X₂ in this case being NH, and hydrogen, both radicals R₁ and R₂ preferably not being H; and R₃ is a residue selected from group consisting of —S—R₆, wherein R₆ is a C1-C30 hydrocarbyl group, at least one of R₁ and R₂ not being H when X₁ and X₂ are oxygen, —S—CH₂—CH(NH₂)(COOH) (cysteine-S-yl), a homologue or derivative (e.g. N-acetyl cysteine-S-yl) thereof, a peptide having up to 200 amino acids which contains at least one amino acid radical with a thiol group, preferably a cysteine radical, and is bonded via the thio sulfur, preferably via the cysteine sulfur (peptide-S-yl), coenzyme A which is bonded via a thiol group or fragments thereof, acyl carrier protein bonded via a thiol group, and dihydrolipoic acid bonded via a thiol group; and pharmaceutically acceptable salts thereof. The present invention also relates to the use of these compounds for preparing a drug, drugs containing the same and their use for the therapy of diseases such as autoimmune disease, NF-kappaB mediated diseases, psoriasis, psoriatic arthritis, neurodermitis, enteris regionalis Crohn, cardiac insufficiency, chronic obstructive pulmonary diseases and asthma and in transplantation medicine.

Provided herein are compositions and methods for improved production of acetyl-CoA and acetyl-CoA derived compounds in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a phosphoketolase (PK), and a functional disruption of an endogenous enzyme that converts acetyl phosphate to acetate. In some embodiments, the host cell further comprises a heterologous nucleotide sequence encoding a phosphotransacetylase (PTA). In some embodiments, the enzyme that converts acetyl phosphate to acetate is a glycerol-1-phosphatase. In some embodiments, the glycerol-1-phosphatase is GPP1/RHR2. In some embodiments, the glycerol-1-phosphatase is GPP2/HOR2. The compositions and methods described herein provide an efficient route for the heterologous production of acetyl-CoA-derived compounds, including but not limited to, isoprenoids, polyketides, and fatty acids.

The present disclosure relates to the use of a switch for the production of heterologous non-catabolic compounds in microbial host cells. In one aspect, provided herein are genetically modified microorganisms that produce non-catabolic compounds more stably when serially cultured under aerobic conditions followed by microaerobic conditions, and methods of producing non-catabolic compounds by culturing the genetically modified microbes under such culture conditions. In another aspect, provided herein are genetically modified microorganisms that produce non-catabolic compounds more stably when serially cultured in the presence of maltose followed by the reduction or absence of maltose, and methods of producing non-catabolic compounds by culturing the genetically modified microbes under such culture conditions.

A method for manufacturing polyhydroxyalkanoate-containing structure, at least a part of a base material surface of the structure being coated with polyhydroxyalkanoate, the method comprises the steps of immobilizing a polyhydroxyalkanoate synthase on the base material surface, synthesizing, on the base material surface, polyhydroxyalkanoate using a 3-hydroxyacyl coenzyme A to become the substrate of the synthase and the synthase and coating at least a part of the base material surface with the synthesized polyhydroxyalkanoate, wherein the synthase contains an amino acid sequence capable of binding to the base material. A polyhydroxyalkanoate-containing structure, at least a part of a base material surface of the structure being coated with a polyhydroxyalkanoate, comprises the base material, a polyhydroxyalkanoate synthase immobilized on the base material surface, and the polyhydroxyalkanoate with which at least a part of the base material surface is coated, wherein the synthase contains an amino acid sequence capable of binding to the base material.

Novel 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase inhibitors are useful as antihypercholesterolemic agents and are represented by the following general structural formulae (I) and (II):

A method for labeling acyl carrier protein (ACP) fusion proteins with a wide variety of different labels is disclosed. The method relies on the transfer of a label from a coenzyme A type substrate to an ACP fusion protein using a holo-acyl carrier protein synthase (ACPS) or a homologue thereof. The method allows detecting and manipulating the fusion protein, both in vitro and in vivo, by attaching molecules to the fusion proteins that introduce a new physical or chemical property to the fusion protein. Examples of such labels are, among others, spectroscopic probes or reporter molecules, affinity tags, molecules generating reactive radicals, cross-linkers, ligands mediating protein-protein interactions or molecules suitable for the immobilization of the fusion protein.

The invention relates to a composition with the efficacies of whitening, moisturizing, repairing and caring the skin, as well as a preparation method and applications of the composition. The skin care composition is prepared from the following raw materials: water, sodium hyaluronate, sodium dihydrogen phosphate dihydrate, potassium chloride, sodium hydroxide, calcium chloride, glutamine, benzalkonium chloride, magnesium sulfate, aminobutyric acid, sodium ascorbate, alanine, arginine, lysine hydrochloride, valine, histidine, leucine, taurine, coenzyme A, alcohol, polysorbate 80, thiamine, disodium diphosphate, recombinant human epidermal cell growth factors and the like. The skin care composition disclosed by the invention can permeate the muscle bottom for replenishing water for the skin from the deep layer, so that the skin is full of elasticity, the color of the skin is brightened, the fine wrinkles are reduced, the skin is clean and watery, the balance of water and oil is regulated, the pores are shrunk, meanwhile, the brightness of the face can be increased, the injured epidermal layer is effectively repaired, the activity of tyrosinase is inhibited, the generation of skin melanin is reduced, so that the skin is healthy, natural and white.

The invention discloses recombinant saccharomyces cerevisiae for producing dammarenediol and protopanoxadiol using xylose and a construction method. The construction method comprises the steps of replacing a promoter of a saccharomyces cerevisiae xylulokinase gene XKS1 with a promoter PFBA1 by virtue of a homologous recombination method, introducing xylose reductase XYL1 and a xylitol dehydrogenase XYL2 expression cassette, increasing the activities of transketolase TKL1 and transaldolase TAL1 so as to obtain recombinant bacteria 1, introducing a farnesyl-diphosphate farnesyltransferase gene ERG9, a squalene monooxygenase gene ERG1 and a dammarenediol synthase gene DS into the recombinant bacteria 1 so as to obtain recombinant bacteria 2, and introducing a nicotinamide adenine dinucleotide-hydroxymethylglutaryl coenzyme A reductase gene NADH-HMGr, farnesyl diphosphatesynthase ERG20 and a protopanoxadiol synthase-cytochrome P450 reductase fusion protein gene PPDS-ATR1 into the recombinant bacteria 2, so as to obtain recombinant bacteria 3. According to the recombinant saccharomyces cerevisiae, dammarenediol and protopanoxadiol can be artificially synthesized by virtue of xylose.

The invention discloses a method for enhancing adipic acid yield in Escherichia coli, belonging to the field of bioengineering. The method is implemented by overexpressing beta-ketothiolase gene, 3-hydroxyacyl-coenzyme A dehydrogenase gene, 3-hydroxyadipyl dehydrogenase gene, 5-carboxyl-2-pentene acylcoenzymea A reductase gene and adipyl coenzyme A in different modules by using atoB-gene-knock-out Escherichia coli BL21 (DE3) as a host; and the adipic acid yield can reach 25.57 g/L. The recombinant bacterium can also perform cyclic fermentation; and after ten cycles, the adipic acid accumulated yield is up to 18.94 g/L. The method has important functions on industrial continuous mass production, can shorten the thallus growth time, and has the advantages of time saving, high speed and cost saving since the cyclic thallus fermentation is directly utilized.

The invention discloses a method for preparing hydroxyalkanoate homopolymer and special bacteria thereof. Recombinant pseudomonas putida is prepared by the method comprising the following steps of: depriving coding functions of 3-hydroxyfatty acyl-coenzyme A dehydrogenase coding gene and 3-ketoacyl coenzyme A thiolase coding gene in pseudomonas putida to obtain recombinant pseudomonas putida, and recording the recombinant pseudomonas putida as recombinant pseudomonas putida I. Experiments prove that the hydroxyalkanoate homopolymer, particularly medium and long-chain hydroxyalkanoate homopolymer (C4-C14), can be obtained by using the recombinant bacteria organism to synthesize polyhydroxyalkanoate. The obtained hydroxyalkanoate homopolymer has high purity and high yield. The method of the invention can synthesize the medium and long-chain hydroxyalkanoate homopolymer with random length, and overcomes the defect that only one 3-hydroxybutyrate (HB) homopolymer can be obtained by biosynthesis in the prior art. Therefore, the method of the invention has broad application prospect in the biosynthesis field of the hydroxyalkanoate homopolymer.

The invention discloses a brewer yeast gene engineering strains for producing miltiradiene, and a construction method and application of the brewer yeast gene engineering strains. The construction method for the gene engineering strains comprises the following steps of introducing exogenous SmCPS (salvia miltiorrhiza copalyl pyrophosphate synthase) and SmKSL (salvia miltiorrhiza kaunene synthase like) into original brewer yeast to obtain recombinant brewer yeasts for producing the miltiradiene, wherein the recombinant brewer yeasts are recorded as ZD-T-000 and ZD-T-010; improving the activity of one or more proteins in 3-hydroxy-3-methylglutaryl coenzyme A reductase, a terpene control element protein UPC2, geranylgeranyl pyrophosphate synthase and farnesyl phosphate synthase on such a basis to construct 24 high-miltiradiene yield recombinant brewer yeast strains (ZD-T-001 to ZD-T-008, ZD-T-011 to ZD-T-018 and ZD-T-021 to ZD-T-028). The brewer yeast ZD-Tans-001 can be used for producing 487.91mg/L miltiradiene after being fermented for 6 days under an aerobic condition.

The invention discloses a haemophilus parasuis culture medium. A preparation method of the haemophilus parasuis culture medium comprises the steps of preparing a basic culture solution, treating coenzyme A, and perfecting a culture medium, wherein the basic culture solution comprises peptone, tryptone, sodium chloride, dextrose, yeast extract, glycerin and distilled water, and the basic culture solution, the coenzyme A and fetal bovine serum are uniformly mixed so as to obtain the haemophilus parasuis culture medium. The haemophilus parasuis culture medium provided by the invention can keep haemophilus parasuis, thereby facilitating the transportation of suspicious haemophilus parasuis samples; and the death of haemophilus parasuis is not caused in the process of transportation, thereby increasing the separation probability of the haemophilus parasuis.

The invention provides a recombinant escherichia coli strain, a preparation method of the recombinant escherichia coli strain and a method for biologically synthesizing poly-3-hydroxypropionic acid from acetyl coenzyme A. The method comprises the following steps: with glucose or glycerin and the like as carbon sources, commonly over-expressing endogenous or exogenous acetyl coenzyme A carboxylase genes (acc) and propionyl coenzyme A synthetase genes (prpE) as well as exogenous malony coenzyme A reductase genes (mcr) and polyhydroxyalkanoate synthetase genes (phaC) in proper host cells (such as escherichia coli), and degrading intermediate products by virtue of glucose so as to biologically synthesize the poly-3-hydroxypropionic acid, wherein acetyl coenzyme A is finally obtained from simple starting materials such as the glucose and the like.

The present invention is directed to compositions for preventing or reducing the flush and other unpleasant symptoms that sometimes accompany the consumption of alcoholic beverages, and methods for use of said compositions. The compositions comprise an agent, or combinations of various agents, such as an agent capable of increasing intracellular or intramitochondrial concentration of NAD in the subject; an agent capable of increasing glutathione concentration in the subject; an anti-oxidant; alpha-lipoic acid or a precursor to alpha-lipoic acid; and/or an agent capable of increasing the level of Coenzyme A in the subject; where the agent or agents are present or administered in amounts effective to prevent or reduce the alcohol reaction in a subject who has consumed one or more alcoholic beverages.

The invention relates to the technical field of landscaping plantation and especially relates to a multiple-effect instillation liquid for trees and a use method thereof. The multiple-effect instillation liquid for trees comprises: coconut, radix astragali and ephedra, Chinese ephedra root, dextrose, coenzyme A, triphosadenine, vitamin B1, vitamin C, dexamethasone sodium phosphate, cytex, brassin, potassium sorbate, boric acid, manganese sulfate, zinc sulfate, an auxin and an antiseptic. The multiple-effect instillation liquid for trees can realize the one-time supply of nutrients to a nursery stock, balancing of moisture of a nursery stock, resistance of water evaporation, fast healing of wounds of a wounded root system, promotion of early rooting, and prevention of infection.

The invention discloses a recombinant strain for producing a 3-hydracrylic acid homopolymer and/or a 3-hydracrylic acid copolymer and an application thereof. The construction method of the recombinant strain comprises the following steps: leading 1,3-Propanediol dehydrogenase coded genes, aldehyde dehydrogenase coded genes, 3-hydracrylic acid coenzyme A ligase coded genes and PHA (Polyhydroxyalkanoates) polymerase coded genes into a starting strain to obtain the recombinant strain. The experiments in the invention prove that the engineering bacteria can efficiently express 3-hydracrylic acid coenzyme A ligase coded genes and PHA polymerase coded genes and enable the 3-hydracrylic acid to be finally polymerized into 3-hydracrylic acid homopolymer (P(3HP)) from the 3-hydracrylic acid coenzyme A. Minitype fermentation tank experiments show that the engineering bacteria provided by the invention can have a maximum P (3HP) output of 8.9g/L after being fermented in a 6L fermentation tank and the P (3HP) can account for a maximum 91.5% of cell dry weight. In addition, the recombinant strain provided by the invention has the advantages of simple production process, low costs and broad application prospects.

A method for constructing genetic engineering bacteria used in the fermentation and the coproduction of PDO, BDO and PHP belongs to the biochemical technical field. The process of the method comprises the following steps that: D-type lactate dehydrogenase gene is removed from wild fungus used for generating PDO, and coenzyme A dependent aldehyde dehydrogenase and polyhydroxy fatty acid synthase gene are introduced so as to construct genetic engineering bacteria used in the fermentation and the coproduction of PDO, BDO and PHP; aerobic fermentation and a fermentation adjust and control mode according to which glycerol and an alkali solution undergo mixing fed batch are adopted; and a product extraction flow during which fermentation broth undergoes membrane filtration, electrodialysis, concentration and rectification steps so as to separate the products of PDO, BDO and PHP. The method has the advantages that: the constructed genetic engineering bacteria can produce PDO, BDO and PHP at the same time, thereby increasing the utilization rate of raw materials and reducing production cost; meanwhile, the synthesis of byproduct lactic acid is reduced, and an after-extraction process is simplified so as to reduce extraction cost; moreover, the method increases the synthesis of thalli NADH2 while introducing PHP.