38 results about "Coenzyme A biosynthesis" patented technology

The invention provides a genetically engineered bacterium for producing O-succinyl-L-homoserine as well as a construction method and application of the genetically engineered bacterium. In a chassis bacterium genome, the expression of a 2-ketoglutaric acid decarboxylase encoding gene sucA is enhanced, and the expression of a succinyl-coenzyme A synthetase encoding gene sucD is weakened, so that the supply of succinyl-coenzyme A is increased; the method comprises the following steps: increasing the NADPH (Nicotinamide Adenine Dinucleotide Phosphate) reducing capacity and ATP (Adenosine Triphosphate) energy supply of a chassis bacterium, increasing DNA (Deoxyribose Nucleic Acid) in combination with a transcription dual regulatory factor ompR to improve the stress resistance of escherichia coli under high osmotic pressure, and introducing an overexpression plasmid containing a homoserine transsuccinylase coding gene metA to construct the genetically engineered bacterium for producing O-succinyl-L-homoserine. The engineering strain obtained through a systematic metabolic engineering modification strategy can realize effective accumulation of OSH, the shake flask yield of OSH reaches 19.8 g / L, the fed-batch fermentation yield of a 5L fermentation tank reaches 110.5 g / L, the sugar-acid conversion rate reaches 52.6%, and a foundation is laid for subsequent construction of high-yield OSH engineering bacteria.

The invention discloses a Chimonobambusa chensinensis CuCCoAOMT1 gene as well as a protein and application thereof, belongs to the technical field of plant genetic engineering, and particularly relates to a Chimonobambusa chensinensis caffeoyl-coenzyme A-O-methyltransferase CuCCoAOMT1 gene, and a nucleotide sequence of the CuCCoAOMT1 gene is shown as SEQ ID NO.1. The invention further discloses a preparation method of the CuCCoAOMT1 gene. The invention further discloses application of the CuCCoAOMT1 gene in regulation and control of the drought resistance of gramineous plants. By overexpressing the caffeoyl-coenzyme A-O-methyltransferase CuCCoAOMT1 gene in the rice, the lignin content in the rice can be effectively increased, the thickening of rice cell walls is promoted, the drought resistance of rice plants is improved, and a new gene resource is provided for plant material property improvement gene engineering.

The invention discloses application of thiamine in preparation of a product for treating subclinical ketosis of dairy cows, and belongs to the field of biological medicines. Tests prove that compared with dairy cows suffering from subclinical ketosis (SCK), by adding thiamine, the content of beta-hydroxybutyric acid (BHBA), the number of bacteria in milk and the number of somatic cells can be remarkably reduced, and the milk yield, the milk fat content, the acetic acid content and the A / P ratio are remarkably increased; the microbial diversity of rumen and excrement of the dairy cow can be adjusted; a metabonomics result shows that metabolite up-regulated after the thiamine is added mainly focuses on pyruvic acid metabolism, glycolysis or gluconeogenesis, tyrosine metabolism, glycerophospholipid metabolism and biosynthetic pathways of phenylalanine, tyrosine and tryptophan. The conclusion is that when thiamine is added into the SCK dairy cow, synthesis and catabolism of BHBA of an organism can be effectively regulated and controlled by adjusting the content of succinyl-coenzyme A, then subclinical ketosis is relieved, rumen fermentation is promoted, and therefore the milk yield and the milk quality are improved.

The present disclosure describes a recombinant yeast host cell having a first engineered metabolic pathway to convert fructose-6-phosphate (F-6-P) into acetyl-coenzyme A. The first engineered metabolic pathway comprises: a genetic modification for expressing a heterologous phosphoketolase capable of converting fructose-6-phosphate into erythrose-4-phosphate, and at least one genetic modification for decreasing native 6- phosphofructo-2-kinase activity classified under Enzyme Commission No. 2.7.1.105. Optionally, the recombinant yeast host cell comprises a second engineered metabolic pathway for convert acetyl-coA into a fermentation product. The present disclosure further describes a process for making a fermentation product. The process comprises contacting the recombinant yeast host cell described herein with a carbohydrate source under a condition allowing the conversion of at least a part of the carbohydrate into a fermentation product.

The invention relates to ribozyme for labeling biotin on target RNA (Ribonucleic Acid) as well as a screening method and application thereof. The RNA labeling technology is one of core tools for modern molecular biology research and clinical diagnosis. A specific marker is covalently linked to a target RNA molecule, so that accurate tracking, efficient detection and functional analysis of RNA can be realized. A biotin-streptavidin system becomes one of the technical routes which are most widely applied due to extremely high affinity and signal amplification capability of the biotin-streptavidin system. At present, common biotin-labeled RNA technical methods mainly comprise a chemical solid-phase synthesis method, an enzymatic method and a chemical labeling method. However, the methods have the limitations of poor site specificity, low efficiency, complex manipulation and the like in the use process. The engineering ribozyme for catalyzing RNA biotinylation is obtained by taking 12: 0 biotin coenzyme A as a biotin donor through an in-vitro screening technology. The ribozyme provided by the invention can efficiently and specifically carry out biotin labeling on target RNA, the reaction process is simple, and the labeling activity is up to 90% or above.

The invention discloses a compound enzyme preparation-containing nutritional feed for regulating and controlling intramuscular fat of black pigs and a preparation method of the compound enzyme preparation-containing nutritional feed, and relates to the technical field of livestock and poultry breeding feeds. The invention discloses a black pig skeletal muscle cell fat directional transport feed which is prepared from the following components in parts by weight: 850-920 parts of basal feed, 5-12 parts of fat directional deposition compound enzyme group, 25-40 parts of amino acid-enzymatic synthesis substrate combination and 2-6 parts of lipolytic enzyme specific inhibitor. According to the black pig skeletal muscle cell fat directional transport feed, fat precursor directional transport is realized through the compounded fat directional deposition compound enzyme group, a fat synthesis pathway targeting black pig skeletal muscle cells and lipoprotein lipase; acetyl-coenzyme A carboxylase and fatty acid synthetase catalyze fat synthesis step by step, only intramuscular fat generation pathways are activated, subcutaneous and abdominal fat deposition paths are blocked, the technical defects that fat deposition of an existing single enzyme preparation is non-directional, and the body fat rate is caused to be abnormally increased are overcome, meanwhile, an exclusive amino acid-enzymatic synthesis substrate combination is matched, and the body fat synthesis efficiency is improved. Sufficient adaptive substrates are supplied to enzyme group catalytic reaction, and the problem that a single enzyme preparation is insufficient in catalytic efficiency is solved.

The invention provides a method for producing DL-2, 4-dihydroxy-3, 3-dimethyl butyric acid (DL-pantoic acid) and / or DL-3-hydroxy-4, 4-dimethyl oxacyclopentane-2-one (DL-pantolactone), in particular to a method for producing DL-2, 4-dihydroxy-3, 3-dimethyl butyric acid and / or DL-3-hydroxy-4, 4-dimethyl oxacyclopentane-2-one (DL-pantolactone) by condensation of hydroxypivalaldehyde and formyl-CoA of C1 source, in particular to a method for producing DL-2, 4-dihydroxy-3, 3-dimethyl oxacyclopentane-2-one (DL-pantoic acid) and / or DL-3-hydroxy-4, 4-dimethyl oxacyclopentane-2-one (DL-pantoic acid). The invention relates to a method for preparing 2, 4-dimethyl oxacyclopentane-2-one. The present invention also provides a genetically engineered microorganism for use in the production of DL-2, 4-dihydroxy-3, 3-dimethylbutyric acid and / or DL-3-hydroxy-4, 4-dimethyl oxacyclopentane-2-one, and also provides a genetically engineered microorganism for use in the production of DL-3-hydroxy-4, 4-dimethyl oxacyclopentane-2-one. In addition, the key enzyme has been engineered to improve its performance in the production of DL-pantoic acid or DL-pantolactone.

The invention relates to a preparation method and application of 6-methyl-3, 5-dioxocapryloyl coenzyme A. According to the preparation method, 2-methylbutyric acid serves as a starting raw material, the 6-methyl-3, 5-dioxocapryloyl coenzyme A is chemically synthesized through the steps of activation, condensation, coenzyme A coupling and the like, and the structure and purity of the 6-methyl-3, 5-dioxocapryloyl coenzyme A are identified through the technologies of LC-MS / MS, 1HNMR and the like. The nine-carbon coenzyme A is successfully prepared and identified through a chemical synthesis method for the first time, the core problem that an intermediate is unstable and cannot be obtained in vitro is solved, and a stable substrate is provided for in-vitro acyl transfer; the QS-21 in-vitro acyl transfer reaction is realized for the first time, an in-vitro catalytic system of a chemical synthesis intermediate and an insect cell expression enzyme is established, and a foundation is laid for development of a QS-21 total synthesis process.

The invention relates to a 5-carboxyl-2-pentenoyl-coenzyme A reductase mutant and application thereof, and belongs to the technical field of biological engineering. Aiming at the problem of low cofactor binding efficiency of 5-carboxyl-2-pentenoyl-coenzyme A reductase in a reverse adipic acid degradation pathway, rational prediction and site-directed mutagenesis experiments are carried out by taking a gene for expressing the 5-carboxyl-2-pentenoyl-coenzyme A reductase as a research object, structural optimization is carried out on a binding site of the enzyme, and the cofactor binding efficiency of the 5-carboxyl-2-pentenoyl-coenzyme A reductase in the reverse adipic acid degradation pathway is improved. According to the method disclosed by the invention, the adipic acid is more suitable for the characteristics of cofactors, so that the binding capacity of the enzyme to the cofactors is enhanced, the activity of catalyzing the reaction of 5-carboxyl-2-pentenoyl-coenzyme A to generate adipoyl coenzyme A is improved, and the yield of the adipic acid is further improved.

The invention provides an application of up-regulated human enoyl coenzyme A delta isomerase 2 (ECI2) in preparation of a medicine for protecting a cardiac function. It is found for the first time that up-regulation of ECI2 promotes degradation of crotonyl coenzyme A by consuming a crotonyl donor (crotonyl coenzyme A) and effectively inhibits crotonyl modification, so that the heart function and myocardial hypertrophy can be effectively improved, and then the heart function protection effect on pressure overload heart diseases and heart failure is achieved. The above findings indicate a huge potential of upregulating ECI2 in the prevention and / or treatment of pressure overload heart diseases and heart failure.

The application discloses a kind of recombination of Pseudomonas putida for producing L-lactic acid using whole-component lignocellulose hydrolysate and its construction method and application, belong to the field of metabolic engineering.The application uses Pseudomonas putida KT2440 as chassis, realizes carbon flow to pyruvic acid convergence and prevents product degradation by knocking out aceEF 、 ppsA And lldD Gene;By in-situ replacement, introduce optimized Lactobacillus plantarum ldhL1 Reconfiguration L-lactic acid synthesis pathway;By integration xylD-yjhH Tandem expression cassette enables xylose utilization;And by quorum sensing promoter P Rox306 Overexpression maeB Realize funnel conversion of lignin monomer.The application innovatively uses lignin derivatives in hydrolysate as natural donor of endogenous acetyl-coenzyme A, makes up lethal growth defect caused by knocking out aceEF The engineering bacteria of the application realize the synchronous high-efficiency conversion of glucose, xylose and aromatic monomer under the condition of not adding exogenous auxiliary carbon source and chemical inducer, which greatly reduces the industrial fermentation cost.

The application belongs to the technical field of diagnostic markers, and particularly relates to a pathological diagnosis biomarker combination for adrenal Cushing syndrome and application. The pathological diagnosis biomarker combination comprises low density lipoprotein receptor (LDLR), 3-hydroxy-3-methylglutaryl coenzyme A synthetase 1 (HMGCS1) and cytochrome P450 11B1 (CYP11B1). The area under the ROC curve (AUC) of the pathological diagnosis biomarker combination can reach 0.881 (95% confidence interval (CI): 0.775-987; sensitivity: 85.7%; specificity: 85.0%), which indicates that the combination of LDLR, HMGCS1 and CYP11B1 as the pathological diagnosis biomarker combination for adrenal Cushing syndrome has high accuracy.

The invention discloses application of a Reg4 gene in treatment of metabolism-related fatty liver, and relates to the technical field of biomedicine. The Reg4 gene is applied to the aspect of treating obesity and metabolism-related fatty liver; according to the application, by increasing expression of the Reg4 gene, liver lipid deposition, liver cell ballooning and inflammatory infiltration related to the metabolism-related fatty liver are inhibited; the application is realized by inhibiting the processes of de novo synthesis of fat in the liver, liver lipid uptake and fatty acid beta oxidation. The step of inhibiting the de novo synthesis of fat in the liver comprises the step of inhibiting the expression of genes of acetyl coenzyme A carboxylase 1, fatty acid synthase and / or stearoyl coenzyme A desaturase 1. The invention definitely proves that the Reg4 gene has an obvious effect of improving obesity and metabolism-related fatty liver, and can relieve hepatic fatty degeneration and inflammation.

The invention discloses a high-stability anti-interference free fatty acid detection kit as well as a preparation method and application thereof, the kit comprises a reagent R1 and a reagent R2, the reagent R1 comprises a phosphate buffer solution, acetyl coenzyme A synthetase, coenzyme A, adenosine triphosphate, 4-aminoantipyrine and a cyclodextrin-metal organic framework nano stabilizer with the concentration of 5-20 mg / mL; and the reagent R2 is prepared from a Tris-HCl buffer solution, acetyl coenzyme A oxidase, peroxidase, N-ethyl-N-(3-sulfopropyl)-3-methylaniline sodium salt and a cyclodextrin-metal organic framework nano stabilizer with the concentration of 2 to 10mg / mL. Through the CD-MOF nano material with the specific aperture, triple breakthrough of improving the enzyme stability, enhancing the interference resistance of a complex sample and optimizing the detection sensitivity is synchronously realized.

The invention discloses a kit for catalyzing Friedel-Crafts acylation reaction through an enzyme method, application and a preparation method. The kit for catalyzing the Friedel-Crafts acylation reaction by the enzyme method comprises 1) a Friedel-Crafts acylation reaction buffer solution and 2) a thiolase complex MucABC or a functional fragment and a variant thereof. The kit for catalyzing the Friedel-Crafts acylation reaction does not depend on coenzyme A compounds as acyl donors, and can realize regional specific C-acylation modification on various lactam compounds by taking acyl phenyl esters with different chain lengths as the acyl donors under mild conditions; a novel green and efficient biological catalysis tool is provided for Friedel-Crafts acylation reaction, and the method has important value and significance for drug synthesis and chemical production.

The application provides a recombinant Halomonas, a construction method thereof and application of the recombinant Halomonas in production of polyhydroxyalkanoate. The recombinant Halomonas expresses 2-keto acid decarboxylase or a 2-keto acid decarboxylase mutant. The application constructs a 3HV synthesis path mediated by 2-keto acid decarboxylase, which can directly convert alpha-ketoglutarate in the tricarboxylic acid cycle into 3-hydroxyvaleryl coenzyme A, realizes de novo synthesis of 3HV monomers, can efficiently utilize conventional carbon sources to synthesize 3HV, breaks through the technical bottleneck of traditional dependence on exogenous propionic acid or valeric acid precursors, realizes obtaining of a higher proportion of 3HV under the condition of no need of adding exogenous precursors, and significantly simplifies the fermentation process and reduces raw material cost and production complexity.

The present application relates to a kind of Candida tropicalis engineering bacteria and its preparation method and the method for treating sewage, belong to genetic engineering technical field.Candida tropicalis engineering bacteria with Candida tropicalis CBW-2 ΔURA3 (preservation number: CGMCC NO.31647) as chassis strain, knock out ACS gene and transfer into promoter heterologous expression alpha-amylase gene;The promoter is endogenous promoter.Candida tropicalis is a kind of unconventional yeast with industrial significance, with the characteristics of fast cell growth, strong environmental tolerance, has the potential of sewage treatment.The present application significantly reduces the activity of acetyl-coenzyme A synthetase by knocking out ACS gene, increases the acetic acid accumulation of strain.And by high-efficiency expression alpha-amylase, starch component can be efficiently converted into acetic acid, can provide acetic acid as carbon source for heterotrophic nitrification-aerobic denitrification strain, without adding exogenous carbon source.

The invention relates to a preparation method of citryl coenzyme A, and belongs to the technical field of biology. The method comprises the following steps: taking citric acid, gastrodin and acetyl coenzyme A as raw materials, and generating citryl coenzyme A under the catalysis of citryl coenzyme A synthetase A obtained by CCSA region coding and citryl coenzyme A synthetase B obtained by CCSB region coding; as the citryl coenzyme A as an intermediate is difficult to separate and does not have a standard substance, the citryl coenzyme A finally passes synthesis indirect verification of a final product parishin C. The parishin C is synthesized through heterologous biology, the controllability is high, the requirement for raw material planting can be reduced, the yield of produced products is high, and the problems of environmental pollution and the like caused by chemical synthesis can be solved. The citryl-coenzyme A synthetase gene is used as a key gene for synthesizing an intermediate of parishin C, assists the synthesis of the parishin C, and can be used for the breeding research of the parishin C.

The application discloses a genetic element for enhancing lactic acid metabolic flux of actinomycetes, and belongs to the field of synthetic biology and microbial metabolic engineering. sucC The application successfully constructs a single expression element and a co-expression element by cloning genes from Streptomyces coelicolor sucD , and verifies that the SucC protein and the SucD protein both have lactic acid coenzyme A synthetase activity through in-vitro protein purification and enzyme activity determination, and the enzyme activity of a protein complex (SucC-SucD, SucCD) expressed by the co-expression element is significantly higher than that of the single protein, indicating that the two proteins have a synergistic effect; the genetic element can efficiently catalyze the combination reaction of lactic acid and coenzyme A in vitro, accelerates the lactic acid metabolic speed, provides a core genetic element for the modification of the lactic acid metabolic pathway of actinomycetes, enriches a synthetic biology tool library, and has important industrial application prospects and academic value.

The invention relates to the technical field of biology, in particular to an enzyme related to synthesis of kaempferol-3-O-(2 '-coumaroyl)-glucoside, a coding gene and application of the enzyme, and discloses acyltransferase CsCFAT, the amino acid sequence of which is shown as SEQ ID NO.1. The acyltransferase CsCFAT effectively solves the problem that existing kaempferol-3-O-(2'-coumaroyl)-glucoside is difficult to obtain. The invention discovers and identifies that the acyltransferase CsCFAT has the function of catalyzing kaempferol-3-O-glucoside and p-coumaroyl-coenzyme A to generate a target product for the first time, and fills the blank of a specific biological catalysis tool of the compound; compared with traditional plant extraction and chemical synthesis, large-scale production of CsCFAT is achieved through prokaryotic expression, a recombinant protein band after purification is single, a single product peak can be generated through catalytic reaction, and the obtaining efficiency and purity of a target compound are greatly improved.

The present application relates to a kind of acyltransferase and its application in the preparation of phenylpropionyl fatty amine compound, the acyltransferase is selected from LbSCT1 And / or LbSCT2, the amino acid sequence of the LbSCT1 As shown in SEQ ID NO:1, the amino acid sequence of the LbSCT2 As shown in SEQ ID NO:2.The present application is screened by acyltransferase in wolfberry, two acyltransferases are successfully identified, both can catalyze coffee acyl-coenzyme A, dihydrocaffeoyl-coenzyme A, p-coumaroyl-coenzyme A, feruloyl-coenzyme A and other phenylpropionyl group and adipylamine, spermine etc.Fatty amine connection, thus establish a kind of efficient with wolfberry spermidine methyl, ethyl, propyl, butyl (Lycibarbarspermidine I-IV) And Kukoamine B as representative phenylpropionyl fatty amine synthesis method, effectively solve the current dihydrocaffeoyl group containing phenylpropionyl fatty amine biosynthesis limited technical bottleneck.

The application discloses a kind of p-coumaric acid or p-coumaric acid derivative production level improved microorganism and its construction method and application, comprising: the genetic target point related to p-coumaric acid metabolic pathway of microorganism is reformed, target point is 4-coumaroyl coenzyme A ligase, hydroxycinnamoyl coenzyme A hydratase lyase, hydroxyacyl coenzyme A dehydrogenase, beta-ketoacyl-coa thiolase, 3- (aryl) propenoate reductase one or more than two combinations, reformation is removed the partial activity or all activity of p-coumaric acid metabolic pathway related genetic target point.The application reduces the degree of degradation of p-coumaric acid by microorganism by reformation p-coumaric acid metabolic pathway related genetic target point, to improve the yield and / or production efficiency of p-coumaric acid and p-coumaric acid derivative produced by microorganism.

The application belongs to the technical field of biological detection, and particularly relates to a probe compound for CoA detection, a fluorescent probe and application, comprising a modified rhodamine series fluorescent dye, a pantothenate kinase inhibitor, a HaloTag, a Nanoluc luciferase and a pantothenate kinase fusion protein; through protein labeling, a series of biosensors are obtained, coenzyme A concentration in living cells can be rapidly detected, and rapid screening of coenzyme A drugs is regulated; through rational design and modification of the chemical probe molecules, and engineering modification of the sensor proteins, the chemical probes can label the sensor proteins in living cells, and then the bioluminescent sensors working in living cells are obtained, the requirements of rapid and high-sensitivity detection of CoA can be met, and the drugs capable of regulating the change of coenzyme A concentration can be high-throughput screened.

The application provides a saccharomyces cerevisiae engineering bacterium for producing squalene through endogenous and artificial synthetic pathways and an application thereof, the saccharomyces cerevisiae engineering bacterium takes ZS00 strain as a starting strain, overexpresses acetyl coenzyme A acetyltransferase gene, hydroxymethylglutaryl coenzyme A synthase gene and N-terminal truncated hydroxymethylglutaryl coenzyme A reductase gene, and effectively improves the yield of squalene. Heterologous expression of mevalonate kinase gene and mevalonate pyrophosphate decarboxylase gene, overexpression of the key gene squalene synthase gene for promoting the conversion of precursors into squalene effectively promotes the synthesis of squalene. The introduction of isopentenol utilization pathway genes and their mutants, through the regulation of IU pathway key substrate, overexpression of isopentenyl pyrophosphate isomerase gene and farnesyl pyrophosphate synthase gene, the final strain can effectively accumulate squalene to 687.93 mg / L. The application realizes the breakthrough of squalene yield and efficiency through complementation and synergistic effect.

Target RNA molecules are labeled through biotin, and accurate tracking and efficient detection of RNA can be achieved. The ribozyme capable of transferring biotin acyl to the 5'terminal of RNA (Ribose Nucleic Acid) is obtained by screening by utilizing an in-vitro screening technology and taking small molecule 12: 0 biotin acyl coenzyme A as a substrate. Then, a conservative motif of the ribozyme is obtained through screening optimization, and a biotin modification site of the ribozyme is determined. The ribozyme is designed into enzyme chain RNA with trans-activity, the biotin modification activity of the ribozyme to substrate chain RNA is verified through EMSA, urea denatured polyacrylamide gel electrophoresis and mass spectrum identification methods, and meanwhile, the transfer site and the molecular weight are determined. The ribozyme obtained by the invention can efficiently and specifically carry out biotin labeling on target RNA, the reaction process is simple, and the labeling activity is up to 90% or above.

The invention belongs to the technical field of gene engineering and enzyme engineering, and particularly relates to hydroxyl o-aminobenzoic acid hydroxyl cinnamyl transferase 5 sourced from oat and application of the hydroxyl o-aminobenzoic acid hydroxyl cinnamyl transferase 5 in synthesis of oat alkaloid. The invention provides a key enzyme, namely hydroxyl anthranilic acid hydroxyl cinnamyl transferase 5 in an oat alkaloid synthesis route and a preparation method of the hydroxyl anthranilic acid hydroxyl cinnamyl transferase 5. The recombinant hydroxy o-aminobenzoic acid hydroxy cinnamyl transferase 5 prepared by the invention can catalyze the synthesis of oat alkaloid in vitro, and specifically, 5-hydroxy o-aminobenzoic acid and coumaroyl coenzyme A or caffeoyl coenzyme A are used as substrates; the oat alkaloid A and the oat alkaloid C can be synthesized under the catalysis of the hydroxyl o-aminobenzoic acid hydroxyl cinnamyl transferase 5, and a theoretical basis and a research direction are provided for developing and utilizing biosynthesis of the oat alkaloid.

The application discloses a polynucleotide sequence for simultaneously reducing blood fat and blood pressure and application thereof. Cell and mouse experiments show that the application can significantly inhibit the expression levels of multiple blood fat and blood pressure molecular targets such as proprotein convertase subtilisin / kexin type 9 (PCSK9), angiotensinogen (AGT) and beta-hydroxy-beta-methylglutaryl-coenzyme a reductase (HMGCR), thereby reducing the blood fat and blood pressure levels of a related disease model mouse. The application is expected to be applied to treating related cardiovascular and cerebrovascular diseases complicated with high blood fat and high blood pressure symptoms.

The invention provides a related medicine for treating senile diseases and application, the related medicine is used for inhibiting or removing methylmalonic acid in a body, and the related medicine is selected from at least one of coenzyme A, hydroxycobalamin, levocarnitine, betaine, carglutamic acid, metronidazole, neomycin, ACSF3 protein or an activator. According to the invention, the level of methylmalonic acid in vivo can be significantly reduced by using a removal technology (hemodialysis or application of a methylmalonic acid scavenger); after the methylmalonic acid is removed, the condition of senescence diseases can be obviously improved; mSC rejuvenation can be significantly increased by inhibiting methylmalonic acid.

The invention discloses a genetically engineered bacterium and application of the genetically engineered bacterium in synthesis of N-acetyl blue, and relates to the technical field of biocatalysis and biosynthesis. On the basis of a gene editing technology, pta, ackA and ldhA genes are knocked out, ACL, Rv2170, panK, panF and ppk genes are integrated, conversion of acetyl coenzyme A in escherichia coli into acetic acid can be blocked, conversion of pyruvic acid into lactic acid can be blocked, supply of coenzyme A and ATP can be enhanced, the concentration of acetyl coenzyme A in a living body is increased, synthesis of N-acetyl observation blue through catalysis of acetyltransferase is promoted, and the synthesis efficiency of N-acetyl observation blue is improved. Therefore, the yield of the N-acetyl blue is improved. According to the genetically engineered bacterium, the N-acetyl observation blue is synthesized through whole-cell catalysis by taking observation blue as a substrate, the problems of low purity and yield of the N-acetyl observation blue in the prior art can be effectively solved, and meanwhile, the genetically engineered bacterium has the advantages of being environmentally friendly, safe, stable and free of chemical pollution.

The application discloses a kit for rapidly detecting bacterial energy metabolism and a preparation method and application thereof, and belongs to the technical field of microorganism analysis, drug screening and detection. The kit comprises reaction premixed powder, anaerobic reaction liquid and optional positive control; the reaction premixed powder comprises the following components in parts by weight: 1 part of benzyl viologen; 0.43-0.64 parts of sodium pyruvate; 0.054-0.082 parts of coenzyme A; 0.037-0.055 parts of MgCl2.7H2O; the anaerobic reaction liquid is a buffer solution containing sodium sulfide-9 hydrate; and the positive control is nitazoxanide or glychalcone A. The application further discloses a preparation method and application of the kit, and the application comprises the following steps: rapidly detecting bacterial energy metabolism activity; and / or screening substances for inhibiting bacterial energy metabolism. The kit can rapidly, sensitively and at low cost evaluate the physiological activity of bacteria, and can also be used for rapidly screening and evaluating anaerobic microorganism energy metabolism inhibitors.