The invention discloses a preparation technology of disodium adenosine triphosphate capable of reducing energy consumption. An appropriate amount of room temperature reverse osmosis water is used for dissolving adenosine to prepare an adenosine solution, the energy consumption is saved by 15% or higher, the operation is simple, and the damage to enzymes is effectively avoided. In the prior art, magnesium sulfate is selectively used as an enzymatic reaction metal ionic compound in a biochemical reaction, but the adsorption capacity of strong basic anion resin used in a subsequent technology for sulfate ions is extremely high, a great difficulty is brought for later resin regeneration treatment, and new foreign ions can be introduced; however, when magnesium chloride is used for replacing the magnesium sulfate, the adsorption capacity of strong basic anion resin for chloride ions is weaker than that of strong basic anion resin for sulfate ions, the later resin regeneration treatment is facilitated, and new foreign ions cannot be introduced. According to the preparation technology, benzalkonium bromide is used as an active agent; the benzalkonium bromide is a nontoxic and innocuous disinfectant , and the difficulty is not increased for later treatment, so that the benzalkonium bromide is a reasonable substitute; a two-stage method is adopted for vacuum drying, so that not only is the pollution avoided, but also the time is saved.