The invention belongs to the technical field of
plant seedling raising, and discloses a rhododendron micranthum seed disinfection and sterile
sowing method, which comprises the following steps: screening seeds to obtain rhododendron micranthum seeds with the purity of more than or equal to 90%; washing with normal-temperature water until the water
absorption rate of the seeds reaches 15-18% Sterile seeds are obtained through
alcohol disinfection and bromogeramine disinfection; inoculating the disinfected seeds into a
germination culture medium, and culturing in an illumination
incubator to obtain aseptic seedlings. According to the method, double disinfection is combined with sterile operation, so that the seed
pollution rate is remarkably reduced, and the disinfection safety is improved; an optimized culture medium formula and precise light and temperature conditions are adopted, so that the seed
germination rate is increased to be greater than or equal to 90%, the
germination time is shortened to 7 days, and the germination is neater and quicker than that of traditional soil
sowing; robust transplantable seedlings can be obtained after sterile culture is performed for 3 months, the
seedling raising period is greatly shortened, meanwhile, the
seedling death risk caused by environment fluctuation and
plant diseases and
insect pests in traditional seedling raising is reduced, and reliable
technical support is provided for efficient breeding of high-quality rhododendron micranthum seedlings.