822 results about "Glycan" patented technology

The invention relates to the field of enzyme engineering, in particular to modified xylanase, a DNA (deoxyribonucleic acid) molecule, a recombinant plasmid and a transfected cell. The modified xylanase protected by the application is formed by replacing a deletion segment of a receptor enzyme with a substitution segment, the receptor enzyme is wild type xylanase, the sequence of the receptor enzyme is SEQ ID NO: 1, the deletion segment comprises a carbohydrate binding module of the receptor enzyme, the substitution segment is a carbohydrate binding module of a donor enzyme, the donor enzyme is a carbohydrate active enzyme, and the donor enzyme is a carbohydrate active enzyme. The sequence of the donor enzyme is one of SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4 and SEQ ID NO: 5. The carbohydrate binding module of the carbohydrate active enzyme with relatively high activity is replaced into the receptor enzyme, so that the binding capacity of the receptor enzyme and the carbohydrate is improved, and the activity of the receptor enzyme is improved.

The present application relates to the technical field of solder paste. The present application discloses a high-precision nano solder paste and a preparation method thereof. The high-precision nano solder paste comprises solder powder, flux, glucomannan and polyethylene glycol modified aliphatic epoxy resin; the raw material composition of the flux comprises: an active agent, a surfactant, a thixotropic agent, an antioxidant, a corrosion inhibitor, a film former and a solvent. The preparation method comprises the following steps: stirring and mixing the active agent and the solvent to prepare a premixed liquid; adding a surfactant, a thixotropic agent, an antioxidant, a film former and a corrosion inhibitor to the premixed liquid in sequence, stirring and mixing to prepare a flux; mixing and stirring the solder powder, flux, glucomannan and polyethylene glycol modified aliphatic epoxy resin, grinding and preparing a high-precision nano solder paste. The high-precision nano solder paste prepared in the present application has good storage stability, long service life, high expansion rate and good wetting performance.

The invention discloses difunctional glucanase for hydrolyzing agricultural and sideline products and application, and belongs to the technical field of biology. The difunctional glucanase is named as RuCel071, and the amino acid sequence of the difunctional glucanase is as shown in SEQ ID NO. 3. The novel cellulase RuCe071 is developed on the basis of a rumen microorganism metagenome resource mining technology, has the activity of simultaneously hydrolyzing cellulose and xylan, can be used for degrading agricultural and sideline products, reducing accumulation of the agricultural and sideline products and environmental pollution, lowering the treatment cost and improving the degradation efficiency, and has the advantages of being simple in preparation process, low in cost and high in application value. The utilization rate and the economic benefit of agricultural and sideline products are favorably improved.

The invention discloses a multifunctional cellulolytic enzyme and application thereof in degradation of agricultural by-products, and belongs to the technical field of biology. The multifunctional cellulolytic enzyme is named RuCelI, and the amino acid sequence of the multifunctional cellulolytic enzyme is as shown in SEQ ID NO. 1. Based on a rumen microbial metagenome resource mining technology, a novel multifunctional cellulose decomposition enzyme RuCelI is developed, and the enzyme has double activities of endo-cellulase and xylanase, can be used for degrading agricultural by-products, reducing the treatment cost and improving the degradation efficiency, and is beneficial to improving the utilization rate and the economic benefit of the agricultural by-products.

The invention discloses an endoglucanase truncated mutant and an application of the endoglucanase truncated mutant. According to the invention, based on endoglucanase BsEGL from bacillus subtilis DLG, a truncated mutant is constructed by deleting a carbohydrate binding domain (CBM) of the endoglucanase BsEGL, and the truncated mutant is named as BsEGL [delta] CBM. Compared with BsEGL, the truncated mutant BsEGL delta CBM has the advantages that the thermal stability, the pH stability, the organic solvent tolerance and the like are remarkably improved, and the activity of degrading phosphoric acid expanded cellulose and corncob xylan is obtained. In addition, the enzymatic activity of the truncated mutant BsEGL delta CBM is remarkably improved by 6.41%, and the catalytic efficiency is remarkably improved. Due to the characteristics, the mutant can be suitable for industrial processes such as feed processing, papermaking, biological energy sources, spinning and food brewing, and is particularly suitable for enzyme catalytic reaction under extreme environments such as high temperature and low pH. The invention provides an enzyme preparation tool with excellent performance for efficient degradation of cellulose biomass, and has important application value.

To provide an imitation shrimp having a distinctive springy texture like that of natural shrimp.SOLUTION: An imitation shrimp comprises a matrix containing glucomannan and a polysaccharide (other than glucomannan) in which plant protein particles containing a seasoning and a colorant are dispersed.SELECTED DRAWING: Figure 1

The invention discloses a radix pseudostellariae extract with high content of radix pseudostellariae cyclic peptide B and a preparation method and cosmetic application of the radix pseudostellariae extract, and belongs to the technical field of cosmetics. The preparation method comprises the following steps: (1) crushing radix pseudostellariae, adding alcohol and performing ultrasonic treatment to obtain feed liquid; (2) adding a compound enzyme into the feed liquid to carry out enzymolysis, enzyme deactivation, solid-liquid separation, concentration and impurity removal to obtain an enzymatic hydrolysate; and (3) eluting the enzymatic hydrolysate with macroporous resin to obtain an eluent, and drying to obtain the product, the compound enzyme is prepared from pectinase, papain, beta-glucosidase, cellulase and xylanase according to the mass ratio of (5 to 10) to (0.5 to 1) to (3 to 7) to (0.3 to 1). The radix pseudostellariae extract disclosed by the invention is prepared by adopting an enzymolysis process, more active ingredients can be reserved, the content of radix pseudostellariae cyclic peptide B in the obtained extract is high, and the radix pseudostellariae extract has relatively good anti-inflammatory, soothing, repairing and anti-aging effects.

The invention discloses an oral monoclonal antibody micro-coated nanopolysaccharide microsphere preparation for targeted therapy of inflammatory bowel diseases and a preparation method thereof. According to the method, oxidized mannan and an anti-TNF-alpha monoclonal antibody are mixed and cross-linked by a cross-linking agent containing a diselenide bond to form the drug-loaded nanoparticles. Mixing the drug-loaded nanoparticles with pectin, dropwise adding the mixture into a calcium chloride solution through an electrostatic spinning device, and carrying out ionic crosslinking to obtain the oral monoclonal antibody micro-coated nano polysaccharide microsphere preparation with the particle size range of 180-200 microns. The drug-loaded polysaccharide microsphere delivers the wrapped drug-loaded nanoparticles to a colitis disease part through electrostatic interaction. The drug-loaded nanoparticles are targeted to macrophages, release of the monoclonal antibody is accelerated under the triggering of high-concentration active oxygen at an inflammation part, and the oral stability and the treatment effect of the monoclonal antibody drug are improved. The polysaccharide is used as a monoclonal antibody targeted delivery carrier, raw materials are cheap and easy to obtain, the preparation process is simple and convenient, reaction conditions are mild, and application and development are easy.

The invention provides a soluble dietary fiber composition. The soluble dietary fiber composition comprises galactomannan, kelp dietary fibers, oat beta glucan, galactooligosaccharide, polydextrose, stachyose and xanthophyll. The invention also provides application of the soluble dietary fiber composition in functional food or constipation treatment medicines.

Provided herein are novel stabiligases for use in cell membrane proteome analysis. The subject stabiligases are capable of attaching to glycans found on the surface of cell membranes to form glycan-tethered (GT) stabiligases. Such glycan-tethered stabiligases are capable of robustly and selectively attaching label probes to cell surface proteins of intact cells. The subject novel stabiligases described herein advantageously allow for the identification and profiling of cell membrane proteins that have undergone an extracellular N-terminal proteolytic event.

The invention relates to the field of extraction of sacha inchi oil, in particular to a preparation method and application of sacha inchi oil, and aims to solve the problems of large loss of heat-sensitive components, solvent residue and low oil yield in the traditional process. The ratio of cellulase to pectinase to xylanase to lipase is 3: 2: 1: 0.5, and carrying out enzymolysis for 2-3 hours at the pH value of 4.5-5.5 and the temperature of 45-50 DEG C; the obtained enzymolysis product is subjected to 28-40kHz ultrasonic treatment for 20-30 minutes, and then is subjected to two-stage gradient squeezing (pre-squeezing at 50-55 DEG C to final squeezing at 35-40 DEG C) in combination with 0.1 mu m ceramic membrane filtration, so that efficient oil extraction and active ingredient protection are realized. The oil yield of the sacha inchi oil is increased to 50.0%, the content of alpha-linolenic acid is larger than or equal to 42%, the content of vitamin E reaches 158 mg / 100 g, no solvent is left, and the sacha inchi oil is suitable for the fields of functional food and cosmetics.

The invention discloses a bi-crosslinking gelatin-based hydrogel wound dressing as well as a preparation method and application thereof, and belongs to the technical field of biomedical materials. The preparation method disclosed by the invention comprises the following steps: mixing a gelatin solution and an oxidized konjac glucomannan solution, heating and stirring to obtain a hydrogel precursor solution; and mixing the hydrogel precursor solution with a borax solution to obtain the bi-crosslinked gelatin-based hydrogel wound dressing. According to the method, oxidized konjac glucomannan and gelatin are subjected to Schiff base and Schiff base crosslinking, borax is further added to introduce a dynamic boric acid ester bond effect to enhance a three-dimensional network of the hydrogel, and the dynamic reversible Schiff base effect and the boric acid ester bond effect are enhanced, so that the pore diameter and the swelling ratio of the hydrogel are increased, and the gel strength of the hydrogel is enhanced; due to the good self-healing injectable performance, biodegradability and mechanical property of the hydrogel, the hydrogel can be used as a wound dressing for irregular deep skin wounds, and the technical problem that existing hydrogel is insufficient in treatment of irregular wounds is solved.

The invention discloses cubilose sialic acid glycan peptide capable of improving lung inflammation and application of the cubilose sialic acid glycan peptide. The ratio of fucose modified sugar chains in the cubilose sialic acid glycan peptide is 30-60%, the ratio of sialic acid modified sugar chains in the cubilose sialic acid glycan peptide is 20-40%, the core structure of the fucose type is (HexNAc) 3 (Man) 3 (Fuc) 1, the core structure of the sialic acid type is (HexNAc) 3 (Man) 3 (Gal) 1 (Fuc) 1 (NeuAc) 1, the neutral sugar dissolution rate of the cubilose sialic acid glycan peptide is high, and the glycopeptide shown in SEQ ID NO 2 has a good inflammation improving effect.

The invention relates to the technical field of dendrobium officinale polysaccharide processing and active ingredient function improvement, in particular to a preparation method of a dendrobium officinale polysaccharide fragment. According to the specific technical scheme, the method comprises the following steps: adding mannase with enzyme activity of 0.7-1U into a dendrobium officinale polysaccharide solution, and stirring for 0.5-1.5 hours; and adding absolute ethyl alcohol until the concentration is 65-75%, centrifuging to remove supernate, collecting precipitate, adding water to redissolve, concentrating, and freeze-drying to obtain the dendrobium officinale polysaccharide fragment. The dendrobium officinale polysaccharide with higher immunoregulatory activity can be conveniently and rapidly obtained in an enzymolysis mode.

Compositions containing a nucleic acid nanostructure having a desired geometric shape and an antigen and / or immunostimulatory agent(s) bound to its surface are provided. The nanostructure design allows for control of the relative position and / or stoichiometry of the immunostimulatory agent(s) bound to its surface. The antigen and / or immunostimulatory agent(s) displayed on the nanostructure surface are arranged with the preferred number, spacing, and 3D organization to elicit a robust immune response. The displayed antigen can be eOD-GT8. The immunostimulatory agent can be, e.g., T cell epitope such as a pan HLA DR-binding epitope (PADRE) and / or a lectin such as MBL or C3, or ligand thereof such as a glycan including mannose. Also provided are antigen-T cell epitope fusions such as eOD-PADRE and nanostructures presenting the same. The immunostimulatory compositions may thus be useful as immunogens, vaccines, adjuvants, and the like. Methods of inducing immune responses are also provided.

The invention relates to the technical field of functional food, in particular to immune regulation type prebiotic syrup with synergy of galactomannan. The active components of the composite solid beverage are prepared from the following raw materials in percentage by weight: 20%-35% of fructo-oligosaccharide, 10%-18% of galactooligosaccharide, 8%-15% of stachyose, 3%-7% of xylooligosaccharide, 5%-12% of lactitol, 5%-10% of galactomannan, 2%-5% of yeast beta-glucan and 1%-3% of N-acetylglucosamine. The composition further comprises 4-8% of inulin and 3-6% of resistant dextrin as a matrix filling and slow-release carrier, and the sum of the weight percentages of all the active ingredients and the carrier is 100%. By remodeling intestinal microecology and inhibiting inflammation pathways, the intestinal inflammation state can be remarkably improved, expression of intestinal mucosa tight junction protein is promoted, and an innovative solution is provided for people suffering from chronic bowel diseases and immune imbalance.

The invention provides a feed for relieving weaning stress of lambs and a preparation method of the feed, and belongs to the field of animal feeds. The feed disclosed by the invention is prepared from the following components in parts by weight: 35-40 parts of puffed corn, 15-20 parts of enzymolysis soybean meal, 5-8 parts of fish meal, 5-8 parts of alfalfa meal, 0.3-3 parts of an organic trace element compound, 0.1-2 parts of a vitamin compound, 3-5 parts of whey powder, 0.5-1 part of butyrin, 0.3-0.5 part of glutamine and 0.2-0.3 part of a radix astragali-spina date seed compound extract. 0.2 to 0.3 part of xylooligosaccharide-xylanase compound, 0.05 to 0.1 part of pancreatin active peptide and 0.1 to 2 parts of complex microbial inoculant. The weaning stress of the lambs is reduced through a targeted intestinal tract-immunity-nerve regulation ternary synergistic formula, the digestive system development of the weaned lambs can be obviously improved, the activity of digestive enzymes can be improved, and therefore the weight gain of the lambs is obviously improved, and the lambs stably pass through the weaning period.

The invention provides a polymorphic zinc and organic acid composition for targeted scalp barrier repair as well as a preparation method and application of the polymorphic zinc and organic acid composition. The composition is prepared from the following components in percentage by mass: 1 to 80 percent of yeast / zinc fermentation product, 1 to 20 percent of polyaspartic acid chelated zinc, 0.5 to 20 percent of gluconolactone, 0.5 to 6 percent of hydroxydecanoic acid, 1 to 10 percent of succinyl glycan and the balance of water or other pharmaceutically / cosmetically acceptable carriers. Wherein the mass ratio of the yeast / zinc fermentation product to the polyaspartic acid chelated zinc is (1: 1)-(8: 1); the mass ratio of the hydroxydecanoic acid to the gluconolactone is (1: 0.5)-(1: 3). The invention provides an efficient, mild and multi-effect synergistic nursing composition, and the composition realizes a three-in-one scalp regulation mechanism of barrier repair, mild bacteriostasis and long-acting moisturizing through unique component synergy.

Preparations of glycan therapeutics, pharmaceutical compositions, dietary supplements and medical foods thereof are provided, and methods of using said gycan therapeutics, e.g. in cancer therapy.

The invention relates to the technical field of toluene degradation treatment, and discloses an immobilized microbial inoculum capable of degrading toluene as well as a preparation method and application of the immobilized microbial inoculum. The preparation method comprises the following steps: adding a bacillus megatherium activated bacterium solution into a liquid culture medium, and adding nano carbon black powder to obtain carbon black immobilized bacteria; heating and stirring the carboxymethyl xylan zinc complex and the nano magnesium lithium silicate until the mixture is molten; after cooling, adding the nano carbon black immobilized bacteria to obtain a mixed solution; slowly dropwise adding the mixed solution into a calcium chloride solution to form embedded pellets; and washing to obtain the immobilized microbial inoculum. According to the immobilized microbial inoculum capable of degrading toluene and the preparation method and application thereof, a strain is embedded in CXY-Zn / nLMS gel and gradually released through gel beads, the biological activity of the strain is better maintained, meanwhile, carbon black of a porous structure provides a place for attachment and growth of the strain, the service life of the strain is prolonged, the degradation capacity of microorganisms is improved, and the immobilized microbial inoculum can be used for degrading toluene. The prepared immobilized microbial agent can be used for sewage treatment and soil improvement.

Provided herein is disclosure of a recombinant bifunctional protein or polypeptide capable of binding to a cell surface receptor for lysosome targeting that is made up of an N-glycosylated peptide comprising at least one N-glycan group and a protein of interest, or antibody or antibody fragment capable of binding to a protein of interest. Also provided herein are methods for producing said recombinant bifunctional protein. Also provided herein are methods for lysosomal degradation of a protein of interest comprising introducing to a cell the peptide sequence of the recombinant bifunctional protein.

The invention discloses a deacetylated konjac glucomannan composite hydrogel and a preparation method thereof, the raw materials comprise deacetylated konjac glucomannan, agar, guar gum and water, the water and polysaccharide powder are mixed according to a certain proportion, magnetically stirred for 15 minutes, then placed in a water bath kettle at 90 DEG C for water bath for 30 minutes, taken out and cooled to room temperature, and the deacetylated konjac glucomannan composite hydrogel is obtained. And refrigerating in a refrigerator at 4 DEG C for 24 hours. The high-elasticity deacetylated konjac glucomannan composite hydrogel is obtained by controlling the adding amount of raw material powder, the deacetylation degree, the stirring time, the water bath temperature and the water bath time, improving the problems of poor gel strength, overlarge viscosity and poor thermal stability of konjac glucomannan single gel and remarkably improving the elasticity and the water-holding capacity of the gel.

The application belongs to the technical field of biology and specifically relates to a method for analyzing glycans on single extracellular vesicles in a fluid sample. The method uses magnetic nanoparticles to capture and manipulate EVs, based on the specific affinity of lectins for specific glycan structures, uses polydisperse microdroplets to separate the captured EVs, realizes local signal amplification in the droplets through enzymatic reaction, adopts fluorescence microscopy to collect images of the droplets, and thus realizes identification and analysis of the glycan components on single EVs. The preparation process of the microdroplets formed by the oscillating emulsification method of the application makes the entire detection process more simple and fast, does not require any microfluidic chip and special device, and can be completed in a centrifugal tube, and all the microdroplets are generated in parallel and quickly, and the amount of microdroplets can be flexibly expanded. Moreover, compared with the traditional ELISA, the method has high sensitivity, low detection limit and small sample consumption.

The invention discloses a frozen surimi antifreeze agent, a preparation method thereof and an application of the frozen surimi antifreeze agent in improving the quality of frozen surimi. The antifreeze agent is prepared from soybean oligosaccharide and konjac glucomannan. According to the present invention, the compound ultrasonic condition is used for surimi freezing, such that the surimi freezing resistance can be significantly improved, the storage quality index of surimi can be well controlled, the surimi protein freezing denaturation can be effectively prevented, and the surimi gel property and the freezing quality can be improved; in the freezing storage process, the quality of the minced fillet treated by the antifreeze agent is obviously better than that of a blank control group. And the freezing deformation of protein in the surimi can be effectively retarded by adding the antifreeze agent and adopting a compound ultrasonic method, the quality of the surimi is ensured, and the shelf life is effectively prolonged. The method disclosed by the invention is convenient to apply, relatively simple in process steps, low in production cost, low in equipment requirement, high in operability and suitable for large-scale industrial application.

The invention discloses a preparation method of a stable Pickering emulsion based on enzymic method modified whey protein microgel particle-araboxylan covalent composite particle, which comprises the following steps: preparing whey protein microgel particles by cross-linking glutamine transaminase, mixing the whey protein microgel particles with an araboxylan solution, and adding an enzyme method modified whey protein microgel particle-araboxylan covalent composite particle to prepare the Pickering emulsion. Adding horse radish peroxidase and a hydrogen peroxide solution to carry out enzymatic modification to obtain a water phase, mixing the oil phase and the water phase according to a volume ratio, carrying out IKA homogenization, and then carrying out ultrasonic treatment to obtain a Pickering emulsion with stable covalent composite particles; the preparation method is simple, harmful substances cannot be generated in the preparation process, and meanwhile, the preparation method is low in production cost, short in production period, rich in nutrition, green, free of pollution and high in economic utilization value; the obtained Pickering emulsion has high viscosity and excellent storage stability and environmental stability, an effective delivery carrier construction strategy is provided for construction of a fat-soluble and heat-sensitive bioactive micromolecule delivery system, and the application range of protein microgel particles in the fields of food, medicine and the like is widened.

The invention provides a radix asparagi homogeneous acidic polysaccharide. The radix asparagi homogeneous acidic polysaccharide contains pectic polysaccharide which is shown as a formula 1, takes rhamnogalacturonidan I (RG-I) as a core and has a homopolygalacturonic acid (HG) structural domain. The invention further provides a preparation method and application of the radix asparagi homogeneous acidic polysaccharide. The asparagi pectin polysaccharide with a new structure is found for the first time, and the asparagi pectin polysaccharide has the effect on acute lung injury. The acidic polysaccharide disclosed by the invention has the advantage of anti-ALI activity, realizes synergistic protection of intestine-lung double target regions, has anti-inflammatory and anti-oxidation double activities, is natural in source and high in biological safety, and is suitable for long-term adjuvant therapy of acute lung injury.

The present invention belongs to the field of biodegradation of mycotoxins, and specifically relates to a complex enzyme preparation for degrading aflatoxins, a complex enzyme preparation solution, and its preparation method and application. The complex enzyme preparation comprises: 35-45 wt% tyrosinase, 20-30 wt% cellulase, 8-12 wt% pectinase, 8-12 wt% xylanase, and 10-20 wt% mushroom laccase. Compared with existing chemical and biological detoxification technologies, the complex enzyme preparation of the present invention contains safe and non-toxic components, is simple to use, and has low economic costs. After treatment with the enzyme preparation solution, the cellulose and hemicellulose in the peanut meal are initially decomposed, making it easier for animals to digest and absorb, and having higher nutritional value and better economic benefits.

The invention discloses a compound enzyme preparation and a preparation method thereof, and relates to the technical field of enzyme preparations. The compound enzyme preparation is at least prepared from the following components in parts by weight: 10 to 30 parts of xylanase, 15 to 35 parts of modified cellulase, 8 to 20 parts of glucanase, 5 to 15 parts of protease, 5 to 18 parts of amylase, 3 to 12 parts of pectinase, 5 to 25 parts of phytase and 15 to 40 parts of modified superoxide dismutase compound microspheres. The compound enzyme preparation provided by the invention is added into daily ration of ruminants, so that the apparent digestibility and growth performance of the ruminants on nutrients can be improved, meanwhile, safer and better meat quality can be obtained, the meat taste is improved, and higher economic value is obtained.

To provide a glycan preparation method for preparing labeled glycans quickly from glycoproteins.SOLUTION: A glycan preparation method comprises: a releasing step in which a glycosidase is allowed to act on a glycoprotein immobilized on a solid phase in a container to obtain a released product containing glycans; and a labeling step in which a labeling reagent is added to the released product in the container to obtain a labeled product containing labeled glycans, wherein the glycoprotein is an antibody, the solid phase has on its surface a ligand selected from the group consisting of protein A, protein G, protein L, protein H, protein D, and protein Arp, and the labeling reagent comprises a reducer, a solution and at least one selected from the group consisting of 8-aminopyrene-1,3,6-trisulfonic acid, a sodium salt thereof, and 9-aminopyrene-1,4,6-trisulfonic acid.SELECTED DRAWING: None

The invention discloses a biomolecular film composition, a skin external preparation as well as a preparation method and application of the biomolecular film composition and the skin external preparation. The composition comprises the following components: 5%-30% of bisabolol; 2%-20% of oryzanol; 0-60% of grease; 0.3%-6% of ethyl cellulose and / or 5%-40% of a rape / tung oil copolymer; 0.5% to 8% of C20-40 acid; 0.1%-1% of a neutralizer; 0.025% to 0.225% of silk fibroin; 0.05% to 0.45% of glucomannan; and 0.1%-1.6% of a preservative. Water-soluble active molecules and oil-soluble active molecules are combined together through hydrophobic chains and hydrophilic chains in fibroin, the composition is prepared through high-pressure homogenization, and the composition has the effects of oxidation resistance, UV damage repair, UV light protection and inflammation resistance and can be adsorbed on the skin surface to form a film; the composition can be used together with a sun-screening agent, so that the sun-screening efficiency and stability of the sun-screening agent are improved, and relatively good water-resistant and sweat-resistant performance is provided.