116 results about "Glycan" patented technology

A thermotolerant xylanase enzyme that is a variant of SEQ ID NO:2, wherein the variant comprises at least one amino acid substitution at a position selected from the group consisting of 90, 105, 114, and 115 and / or comprises any of the following sets of substitutions: (a) G48C and T206C; (b) Y123V, S125C and N171C; (c) S114C, Y123V, S125C and N171C; (d) Y123V, S125C, N171C, G48C and T206C; (e) S114C, Y123V, S125C, N171C, G48C and T206C; wherein the substitution or substitutions increases the thermotolerance of the xylanase relative to a parent xylanase lacking the substitution(s).

This invention discloses an arabinogalactan sulfate derived from *Streptococcus filamentosa*, its preparation method, and its applications, belonging to the fields of natural product chemistry and marine biological resource development technology. The technical solution includes an arabinogalactan sulfate derived from *Streptococcus filamentosa*, with a monosaccharide composition comprising arabinose, galactose, and rhamnose, the molar percentage of arabinose being not less than 70%; the main chain backbone is modified with natural sulfate groups, with the sulfate groups replacing at least one hydroxyl site of the arabinose residues. This invention, when applied to anticoagulation, exhibits good safety and a significant anticoagulant effect.

In a method for treating cancer by desialylation of sialylated glycans expressed on the surface of cancer cells, the present invention provides a method for extending the serum half-life of the active ingredient, sialidase, and enhancing the therapeutic effect of said sialidase. [Solution] A pharmaceutical composition is provided that contains sialidase which, when administered to a subject, conjugates to a serum half-life enhancing factor that increases the serum half-life of sialidase.

This invention relates to the field of fertilizer technology, specifically to an organic liquid fertilizer based on straw enzymatic hydrolysate and its preparation method. The method includes: pretreating corn straw with potassium hydroxide, followed by enzymatic hydrolysis with cellulase and xylanase to obtain the enzymatic supernatant; sequentially treating the supernatant with an ultrafiltration membrane with a molecular weight cutoff of 3000 Da and a nanofiltration membrane with a molecular weight cutoff of 500 Da to collect oligosaccharides with a molecular weight window of 500-3000 Da; partially stabilizing the reducing end of the oligosaccharides with sodium borohydride; pre-complexing with boric acid at pH 8.4-8.8; then sequentially adding relatively low molecular weight sodium polyglutamate, calcium chloride dihydrate aqueous solution, and ultra-high molecular weight sodium polyglutamate to construct a sequential complexation system; subsequently adding potassium nitrate and citric acid aqueous solution; and finally, after filtration at 5 μm and heat treatment at 70℃, obtaining the organic liquid fertilizer. This fertilizer has a high proportion of 500-3000 Da oligosaccharides and a low reducing sugar equivalent, inhibiting storage precipitation and browning, and improving the retention rate of extractable oligosaccharides in the soil.

The application discloses a kind of high toughness biomass-based composite film for liquid packaging and its preparation method and application, belong to biomass-based packaging material technical field.The composite film is ABA three-layer co-extrusion structure;A layer is composed of modified PLA, PBAT and lignin-containing cellulose nanofiber;B layer is composed of oligosaccharide modified polyester;A layer master batch and B layer material are prepared respectively, and three-layer co-extrusion molding is obtained.The application uses amide bond to covalently fix oligosaccharide on polyester main chain, solves the problems of existing technology, such as easy migration of antibacterial agent, weak interlayer bonding force and insufficient comprehensive performance;Through multivariate blending, the synergistic improvement of mechanical property and barrier property is realized by using multiple intermolecular forces;ABA three-layer co-extrusion structure is used, and interface firm combination is realized by means of interlayer molecular diffusion and hydrogen bond network.The obtained composite film has high toughness, excellent barrier property and antibacterial property, and is suitable for milk, fruit juice and other liquid food packaging.

The application discloses a konjac glucomannan-based temperature-sensitive and targeted microsphere probiotic embedding method and relates to the technical field of biochemistry, and comprises the following steps: step 1, a free radical copolymerization method is used to synthesize copolymer microspheres; step 2, amino modification is performed to prepare amino groups; step 3, the amino groups are grafted onto the copolymer microspheres through an epoxy ring-opening reaction to prepare copolymer microspheres; step 4, monoclonal antibody copolymer microspheres are prepared; and step 5, the copolymer microspheres are mixed with probiotic hyphae to complete embedding of the probiotics, and monoclonal antibody-probiotic copolymer microspheres are obtained. The konjac glucomannan-based temperature-sensitive and targeted microsphere probiotic embedding method is a four-component composite carrier which is innovative in terms of a skeleton, temperature sensitivity, crosslinking and antibody targeting, and is innovative in a step-by-step grafting and temperature-sensitive and targeted synergistic process, solves the pain points of poor protection and difficult targeted release of the probiotics, and realizes precise and intelligent delivery.

The application discloses a high-stability immobilized mannanase and a preparation method and application thereof, and belongs to the technical field of enzyme immobilization. The preparation method uses PAMAM modified SBA-15 / calcium alginate composite microspheres as a carrier to fix mannanase in an enzyme solution by adopting an embedding-covalent crosslinking method, so as to obtain the high-stability immobilized mannanase. The preparation method uses the PAMAM modified SBA-15 / calcium alginate composite microspheres as the carrier to fix the mannanase by adopting the embedding-covalent crosslinking synergistic method, so that the pH tolerance, the circulation stability and the hypoglycemic efficiency of the enzyme are improved, and the problems of the traditional immobilized enzyme, such as large enzyme activity loss, easy leakage and poor stability under high temperature, are solved.

Tumor glycocalyx may be regarded as a glycan “canopy” above the tumor plasma membrane. Disclosed are methods of treating a neoplastic malignant cell cancer wherein the neoplastic malignant cancer cell has a malignant glycocalyx B. Also disclosed are methods of inducing apoptosis of one or more neoplastic malignant cells and methods of increasing a subject's responsiveness to an immunotherapy wherein the neoplastic malignant cancer cell has a malignant glycocalyx B.

The application discloses a kind of fermented whipped cream and method prepared by using two-phase oil gel, belong to fermentation milk processing technical field.The application is prepared oil phase gel with enzymolysis coconut oil, rapeseed protein as raw material, and prepared water phase gel with selenized carrageenan and oxidized konjac glucomannan, then two-phase oil gel is formed by processing;Cream raw material is pre-fermented by saccharomyces cerevisiae, and cream microspheres are obtained by high-pressure homogenization, then fermented whipped cream is obtained by mixing two-phase oil gel and compound lactic acid bacteria.The application constructs stable crosslinking network by cysteine, gives two-phase oil gel good elasticity and stability, cream microspheres can avoid sedimentation and aggregation, and compound lactic acid bacteria fermentation not only promotes the system to form uniform gel network, but also retains characteristic flavor.The fermented whipped cream prepared by the application has uniform texture and rich and lasting flavor, has nutrition and taste advantage, can effectively solve the problem of loose structure and easy flavor loss of traditional fermented whipped cream, and has good industrial application prospect and market value.

This invention discloses a method for preparing fucoidan from *Sargassum hemifolia* and its application in the preparation of anti-hepatocellular carcinoma drugs. Experimental results show that *Sargassum hemifolia* fucoidan Fb has a strong inhibitory effect on HepG2 hepatocellular carcinoma cells, significantly stronger than the inhibitory effects of *Sargassum henryi* polysaccharide Fh and *Sargassum fusiforme* polysaccharide SFPS on HepG2 hepatocellular carcinoma cells. After treatment of HepG2 hepatocellular carcinoma cells with *Sargassum hemifolia* fucoidan DF1 and DF2, apoptosis is ultimately induced through the interaction and cooperation of exogenous (death receptor pathway) and endogenous (mitochondrial pathway). *Sargassum hemifolia* fucoidan possesses strong antitumor activity and therefore can be used to prepare anti-hepatocellular carcinoma drugs.

This invention discloses a sialyl polysaccharide-sialic acid-binding immunoglobulin-like lectin signaling pathway blocker, containing one or more N-acetylneuraminic acid derivatives, used to manufacture immunotherapy synergistic drugs or immunomodulatory drugs. The immunotherapy synergistic drugs are used in combination with immunotherapy drugs, and the immunomodulatory drugs are used to enhance the regulation of immunity.

A food or beverage ingredient is produced from enzymatically hydrolysed cereal, by contacting a slurry of cereal bran with an enzyme composition including xylanase, alpha-amylase, beta-glucanase, cellulase and endoprotease. A beverage creamer composition may contain the food or beverage ingredient.

This invention discloses a forest-grown Panax notoginseng extract, an oral liquid, its preparation method, and its application, belonging to the field of Panax notoginseng processing technology. The preparation method includes the following steps: (1) fresh forest-grown Panax notoginseng is crushed with water to obtain a Panax notoginseng suspension; (2) a complex enzyme is added to the Panax notoginseng suspension for enzymatic hydrolysis to obtain an enzymatic hydrolysate; (3) the enzyme inactivation of the hydrolysate, solid-liquid separation, and concentration of the clear liquid are obtained; the complex enzyme is cellulase, xylanase, and flavor protease, and the mass ratio of cellulase, xylanase, and flavor protease is 10-20:3-7:2-6. The Panax notoginseng extract prepared by enzymatic hydrolysis using forest-grown Panax notoginseng as raw material has the effects of assisting in lowering blood pressure and enhancing immunity. Compared with conventional Panax notoginseng extracts, this invention has a better effect in assisting in lowering blood pressure.

The present application relates to the field of enzyme engineering, and in particular to a mutanase and application thereof. At present, mutan is mostly prepared by bacterial extraction, and there are problems such as low product yield and many impurities. The present application provides a mutanase, a MutI gene fragment of the mutanase is obtained by cloning a genome of Leuconostoc pseudomesenteroides G496, and a genetically engineered bacterium is constructed. The mutanase provided by the present application is subjected to enzymatic property analysis, and it is found that the mutanase has good stability under acidic conditions, and the optimal induction expression temperature and reaction temperature are close to room temperature, and the production energy consumption is low and the raw material conversion rate is high. The catalytic product of the mutant enzyme is linear mutan with a high polymerization degree, the molecular weight of the linear mutan is as high as 650 kDa, the linear mutan contains 90% of alpha-1,3 glycosidic bonds, and the structure is relatively dense, and the linear mutan has a good application prospect in the field of biological base materials.

The present invention relates to a novel polypeptide which displays specific carbohydrate binding activity, particularly against glycans that contain mannose, and in vivo and ex vivo methods of use thereof. Methods of use comprise administering a polypeptide of the invention, for example to a sample or a subject in which carbohydrate is present, under conditions suitable for the polypeptide to bind the carbohydrate. The carbohydrate may be expressed by a pathogen. The invention relates to polypeptides and compositions thereof with anti-pathogen activity, such as anti-viral, anti-bacterial, anti-fungal or anti-tumour activity. Also provided are methods for the prevention or treatment of diseases and conditions mediated by pathogens which express carbohydrate, for example as glycoproteins.

The present application provides a soluble dietary fiber composition, its preparation method and application. After the soluble dietary fiber composition enters the colon, the ester bond in the alanine modified pectin is rapidly degraded, part of the chlorogenic acid is released, the secretion of anorectic hormone is stimulated, the appetite is reduced, and the heat intake is reduced; the remaining amide bond network is slowly degraded, realizing the slow and continuous release of chlorogenic acid. Lutein removes free radicals generated by fat decomposition, reducing the oxidative damage of L cells. Galactomannan and alanine modified pectin-glucan modified protein-chlorogenic acid ternary complex synergize to improve the molar ratio of butyric acid / propionic acid: propionic acid enhances satiety through intestinal gluconeogenesis, and butyric acid stimulates the secretion of GLP-1 / PYY, realizing endogenous appetite suppression. Galactomannan absorbs water to form a lubricating gel, softening the stool and increasing the stool volume; the ternary complex degrades to produce short-chain fatty acids, optimizing the intestinal microenvironment and enhancing gastrointestinal peristalsis, both of which synergistically improve constipation.

The application provides a preparation method of anti-aging wheat starch; high-temperature-resistant alpha-amylase, papain and saccharifying enzyme are used for enzymolysis of young leaves of highland barley; the product after the enzymolysis is subjected to first solid-liquid separation, and residues are collected; the residues are added with xylanase and cellulase for enzymolysis; the product is cooled to room temperature and subjected to second solid-liquid separation; polysaccharide in the liquid is precipitated by ethanol; the precipitate is redissolved, dialyzed, concentrated by rotary evaporation and vacuum freeze-dried to obtain polysaccharide; the polysaccharide is dissolved in water to obtain a uniform polysaccharide solution; wheat starch is added into the polysaccharide solution; the mixture is stirred and uniformly mixed to obtain a mixed system; the mixed system is heated and gelatinized; the product is obtained by cooling at room temperature and freeze-drying; and the anti-aging wheat starch prepared based on the polysaccharide obtained from the enzyme residue can be used for making various snacks, such as crystal moon cake crust, shrimp dumplings, powder cakes, rice rolls and the like, and can enhance the anti-aging function of food.

The application discloses a method for separating and extracting xylanase based on a deep eutectic solvent (DES) and a two-water-phase system, and belongs to the technical field of biological separation engineering and enzyme purification. The method comprises the following steps: first, preparing a deep eutectic solvent by mixing and heating betaine (Bet) and 1,2-propylene glycol (1,2-Prop); second, mixing the deep eutectic solvent, inorganic salt and water in a certain proportion to build a two-water-phase system, wherein the mass percentage of the deep eutectic solvent is 40%, and the dosage of the inorganic salt is 0.2 g / mL; and third, adding a hydrolyzate containing xylanase into the two-water-phase system to perform extraction separation. The two-water-phase system formed by the deep eutectic solvent and the inorganic salt is used to selectively distribute the xylanase, the extraction rate can reach more than 90%, and the enzyme activity retention rate is greater than 85%. Compared with a traditional polymer two-water-phase system, the method has the advantages of high separation efficiency, mild conditions, small enzyme activity loss, low cost, green environmental protection, simple operation and the like, and is suitable for industrialized separation and purification of xylanase.

The present invention relates to a method for purifying labeled glycans by electroosmotic flow assisted capillary zone electrophoresis, or a method for on-line electrokinetic purification of a labeled reaction mixture of labeled glycans and free labeling agent, wherein the method is performed in a separation mode, wherein the free labeling agent and the labeled glycans migrate in opposite directions, thereby separating the free labeling agent from the glycans. The method according to the present invention allows for a purification-free capillary zone electrophoresis analysis of labeled glycans. Furthermore, the method allows for separation in a shorter analysis time without the need for a sample purification step.

Recombinant yeasts bioengineered to overexpress genes for utilization of cellulosic and hemicellulosic fibers and / or produce lipid, and methods of use thereof. The yeasts are modified to express, constitutively express, or overexpress an exo-cellobiohydrolase I, an exo-cellobiohydrolase II, an endoglucanase, an endoxylanase, or any combination thereof. The yeasts may also be modified to express, constitutively express, or overexpress a lytic polysaccharide monooxygenase, an arabionofuranosidase, a xylanases, an acetylxylan esterase, or any combination thereof. The methods include cultivating the recombinant yeasts to convert low value organic stillage byproducts such as cellulose and hemicellulose into lipids suitable for biodiesel production and other higher value uses.

This application discloses a muscle stem cell resuscitation and protection washing solution, a quality assessment kit, and a method of use, belonging to the field of cell biology technology. In this application, vitamin E succinate and mannan peptide are used as core antioxidant components, synergistically combined with Gynostemma pentaphyllum polysaccharide, Portulaca oleracea polysaccharide, trehalose, L-proline, and magnesium sulfate heptahydrate to construct a four-in-one resuscitation and protection washing solution that provides isotonic buffering, extracellular protection, intracellular protein stabilization, antioxidant effects, and mitochondrial stabilization. This increases the survival rate of muscle stem cells after resuscitation to over 93%. The kit integrates a multi-dimensional detection system for cell resuscitation and protection washing solution, cell viability, proliferation rate, Pax7 / MyoD double-positive stemness assessment, and cryopreservation damage, achieving closed-loop management of the entire process of muscle stem cell resuscitation, protection, and quality assessment. The double-positive rate of stemness markers reaches over 90%, providing reliable quality assurance for cell bank management and clinical applications.

The present application belongs to the technical field of washing liquid, and particularly relates to a mild laundry liquid for low-temperature stain removal based on microcapsule embedding technology. The laundry liquid is composed of microcapsules and laundry liquid base liquid. The microcapsules are composed of the following raw materials: a biological enzyme system, a polyol protective agent, a sugar-protein protective agent, a complexing agent, calcium lactate, an interface booster, sodium alginate, a polysaccharide, CaCl2, hydroxyethyl cellulose and PEG-400. The laundry liquid base liquid is composed of the following raw materials: a mild surfactant, a complexing agent, PEG-400, polyvinylpyrrolidone and a thickening agent, and the rest is deionized water. The present application realizes the unification of low-temperature high-efficiency stain removal and mild washing and protection in a four-layer structure. The core phase protects the enzyme and resists the interference of hard water. The double-triggered shell resolves the contradiction between storage stability and fast release in washing, the outer layer promotes the dispersion and mass transfer of the capsule, the mild base liquid reduces the damage to the fabric, and the multiple structures are cooperatively adapted to the low-temperature fast washing scene.

The present invention aims to provide an intraoral adhesive film that combines adhesive properties and slow dissolution properties in a well-balanced manner. [Solution] The present invention provides an intraoral adhesive film containing (A) ιcarrageenan and (B) κcarrageenan. Preferably the film further contains (C) a water-soluble polymer such as tamarind gum, gum arabic, karaya gum, xanthan gum, gellan gum, tragacanth gum, guar gum, tara gum, locust bean gum, pullulan, gelatin, casein, pectin, agar, glucomannan, galactomannan, starch, carboxymethyl starch, dextrin, alginic acid, alginic acid ester, chitin, chitosan, carboxymethylcellulose, λcarrageenan, hydroxyethylcellulose, hydroxypropylcellulose, hydroxypropylmethylcellulose, methylcellulose, and ethylcellulose.

The application provides a sensor with antioxidant and antifouling properties and a preparation method and application thereof, and belongs to the technical field of marine chemistry. The sensor takes a glassy carbon electrode (GC) as a substrate, adopts polyaniline as a solid contact layer, and takes a calcium ion selective membrane (ISM) as a detection core. The innovation of the sensor is that a fucoidan-based functional polypeptide (PFp) is modified on the ISM to construct an antifouling layer. The PFp layer forms a triple synergistic defense mechanism of "anti-adhesion-sterilization-antioxidation". Experiments prove that the sensor can effectively inhibit the adhesion and colonization of bacteria such as escherichia coli and staphylococcus aureus and chlorella, and significantly slow down the oxidative decay of the sensing membrane. In addition, when the sensor is used for calcium ion detection in actual seawater, it exhibits excellent long-term stability and high precision, and provides an innovative strategy for continuous in-situ monitoring in complex media.

A protein-glycan conjugate having multiple payloads. By means of a site-specific glycan conjugation technique, different types of payloads are conjugated to specific positions of a protein-glycan chain, so as to obtain a conjugate having multiple payloads. The conjugate exhibits good biological effects.

The application discloses a cell PD-L1 glycosylation in-situ visualization and biological function research method based on hybridization chain reaction. The application adopts a hybridization chain reaction strategy (HCR) of a logic "AND" gate activated by nucleic acid aptamer recognition and metabolic glycan labeling, and constructs a new method for a cell PD-L1 glycosylation in-situ visualization and biological function research method. Through HCR signal amplification induced by adjacent double recognition, the detection sensitivity of PD-L1 glycosylation on different cells is significantly improved, and subtle fluctuations in the PD-L1 glycosylation level can be accurately captured, so as to provide an analysis tool for revealing the dynamic changes under different physiological or pathological conditions. Meanwhile, the application first clarifies the immune regulation function of the PD-L1 glycosylation of dendritic cells, and further expands the research scope of tumor immune checkpoints.

This invention relates to the field of sports nutrition food technology, and discloses a multivitamin formula and its preparation method, comprising the following components in parts by weight: 1.8-2.2 parts of mannose oligosaccharide, 1.8-2.2 parts of galacose oligosaccharide, 9-11 parts of xylooligosaccharide, 6.5-7.5 parts of fructooligosaccharide, 8.5-9.5 parts of maltitol, 1.8-2.2 parts of erythritol, 2.8-3.2 parts of galacose-mannan, 1.8-2.2 parts of stachyose, 13-15 parts of inulin, 38-42 parts of resistant dextrin, 0.20-0.24 parts of vitamin C, 0.45-0.55 parts of silicon dioxide, 0.010-0.014 parts of vitamin B1, and vitamin B2. 0.005-0.007 parts, folic acid 0.0009-0.0011 parts, γ-aminobutyric acid 0.45-0.55 parts, tyrosine 3.8-4.2 parts, magnesium lactate 3.8-4.2 parts; This invention forms a complex prebiotic system by simultaneously adding eight kinds of dietary fiber / oligosaccharides: resistant dextrin, inulin, xylooligosaccharide, fructooligosaccharide, galactooligosaccharide, mannose, galactomannan, and stachyose, which comprehensively regulates the intestinal flora and promotes the production of short-chain fatty acids.