995 results about "Glycan" patented technology

The invention discloses a compound enzyme preparation for improving the absorption and utilization rate of a fertilizer and a preparation method thereof, and belongs to the technical field of enzyme preparations. The compound enzyme preparation is prepared from the following components in parts by weight: 100 parts of purified water, 6 to 8 parts of modified cellulase, 10 to 12 parts of immobilized protease, 10 to 12 parts of phosphatase, 10 to 14 parts of urease, 8 to 10 parts of nitrate reductase, 10 to 12 parts of pectinase, 8 to 10 parts of chitinase, 6 to 8 parts of xylanase, 10 to 12 parts of beta-glucosidase, 10 to 12 parts of superoxide dismutase and 4.5 to 6 parts of a microbial agent, the modified cellulase is obtained by chemically modifying cellulase; the immobilized protease is prepared by the following steps: adsorbing activated and coupled modified polylactic acid and a protease solution, crosslinking with glutaraldehyde, filtering, washing and drying. The compound enzyme preparation improves the fertilizer absorption and utilization rate of plants.

The invention relates to the field of cubilose peptides, in particular to preparation and application of cubilose glycopeptides capable of preventing and assisting in treating gynecological inflammation and gastrointestinal inflammation. The invention provides cubilose glycopeptide which comprises cubilose peptide, sialylated O-glycan and sialylated N-glycan, and the cubilose peptide is connected with the sialylated O-glycan and / or the sialylated N-glycan. The cubilose glycopeptide provided by the invention has unique sialylated O-glycans and N-glycans, has unique biological activity and functions and synergistic effect compared with the cubilose glycopeptide without N-glycans or O-glycans and non-sialylated cubilose peptides, and forms and enhances the inhibition effect on pathogens and the antiviral effect; therefore, the compound has unique advantages in the aspect of preventing and treating related problems. The sialic acid binding type cubilose glycopeptide has stronger antibacterial activity, especially has an inhibition effect on pathogens such as candida albicans and helicobacter pylori, and also has an antiviral effect.

The invention relates to the field of enzyme engineering, in particular to modified xylanase, a DNA (deoxyribonucleic acid) molecule, a recombinant plasmid and a transfected cell. The modified xylanase protected by the application is formed by replacing a deletion segment of a receptor enzyme with a substitution segment, the receptor enzyme is wild type xylanase, the sequence of the receptor enzyme is SEQ ID NO: 1, the deletion segment comprises a carbohydrate binding module of the receptor enzyme, the substitution segment is a carbohydrate binding module of a donor enzyme, the donor enzyme is a carbohydrate active enzyme, and the donor enzyme is a carbohydrate active enzyme. The sequence of the donor enzyme is one of SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4 and SEQ ID NO: 5. The carbohydrate binding module of the carbohydrate active enzyme with relatively high activity is replaced into the receptor enzyme, so that the binding capacity of the receptor enzyme and the carbohydrate is improved, and the activity of the receptor enzyme is improved.

The present disclosure relates to a low glycosylated spike protein and a vaccine designed to express the spike protein in vivo. The present disclosure also teaches a method for generating an immune response by utilizing the low glycosylated spike protein, which provides a broader protection across different variants. A method for identifying a glycan-shielded conserved peptide of a glycoprotein is also disclosed.

The present application relates to the technical field of solder paste. The present application discloses a high-precision nano solder paste and a preparation method thereof. The high-precision nano solder paste comprises solder powder, flux, glucomannan and polyethylene glycol modified aliphatic epoxy resin; the raw material composition of the flux comprises: an active agent, a surfactant, a thixotropic agent, an antioxidant, a corrosion inhibitor, a film former and a solvent. The preparation method comprises the following steps: stirring and mixing the active agent and the solvent to prepare a premixed liquid; adding a surfactant, a thixotropic agent, an antioxidant, a film former and a corrosion inhibitor to the premixed liquid in sequence, stirring and mixing to prepare a flux; mixing and stirring the solder powder, flux, glucomannan and polyethylene glycol modified aliphatic epoxy resin, grinding and preparing a high-precision nano solder paste. The high-precision nano solder paste prepared in the present application has good storage stability, long service life, high expansion rate and good wetting performance.

The invention discloses difunctional glucanase for hydrolyzing agricultural and sideline products and application, and belongs to the technical field of biology. The difunctional glucanase is named as RuCel071, and the amino acid sequence of the difunctional glucanase is as shown in SEQ ID NO. 3. The novel cellulase RuCe071 is developed on the basis of a rumen microorganism metagenome resource mining technology, has the activity of simultaneously hydrolyzing cellulose and xylan, can be used for degrading agricultural and sideline products, reducing accumulation of the agricultural and sideline products and environmental pollution, lowering the treatment cost and improving the degradation efficiency, and has the advantages of being simple in preparation process, low in cost and high in application value. The utilization rate and the economic benefit of agricultural and sideline products are favorably improved.

The invention discloses a multifunctional cellulolytic enzyme and application thereof in degradation of agricultural by-products, and belongs to the technical field of biology. The multifunctional cellulolytic enzyme is named RuCelI, and the amino acid sequence of the multifunctional cellulolytic enzyme is as shown in SEQ ID NO. 1. Based on a rumen microbial metagenome resource mining technology, a novel multifunctional cellulose decomposition enzyme RuCelI is developed, and the enzyme has double activities of endo-cellulase and xylanase, can be used for degrading agricultural by-products, reducing the treatment cost and improving the degradation efficiency, and is beneficial to improving the utilization rate and the economic benefit of the agricultural by-products.

The invention discloses a kind of eucommia leaf fermentation extract and its preparation method and application, and belongs to the technical field of microorganisms and feed additives. With eucommia leaf and wheat bran as substrates, by configuring a specific black aspergillus niger-trichoderma harzianum-saccharomyces cerevisiae complex bacterial liquid as a fermentation agent, a closed fermentation is carried out to make black aspergillus, trichoderma harzianum, and saccharomyces cerevisiae ferment to produce a large amount of cellulase, protease, lipase, amylase and glucosidase, etc., and then by pumping and circulating treatment, the cellulose, protein, starch, beta-glycan and other macromolecular substances in eucommia leaf are further enzymolyzed into protein oligopeptides, monosaccharides, and soluble oligosaccharides that are beneficial to livestock and poultry absorption. The eucommia leaf fermentation extract prepared by the present invention is used as a feed additive for beef cattle feeding, improves the daily weight gain and feed intake of beef cattle, and significantly reduces the feed-to-meat ratio, saves feed consumption, improves the protein content in meat, reduces the fat content, reduces energy, and improves meat quality.

The invention discloses an endoglucanase truncated mutant and an application of the endoglucanase truncated mutant. According to the invention, based on endoglucanase BsEGL from bacillus subtilis DLG, a truncated mutant is constructed by deleting a carbohydrate binding domain (CBM) of the endoglucanase BsEGL, and the truncated mutant is named as BsEGL [delta] CBM. Compared with BsEGL, the truncated mutant BsEGL delta CBM has the advantages that the thermal stability, the pH stability, the organic solvent tolerance and the like are remarkably improved, and the activity of degrading phosphoric acid expanded cellulose and corncob xylan is obtained. In addition, the enzymatic activity of the truncated mutant BsEGL delta CBM is remarkably improved by 6.41%, and the catalytic efficiency is remarkably improved. Due to the characteristics, the mutant can be suitable for industrial processes such as feed processing, papermaking, biological energy sources, spinning and food brewing, and is particularly suitable for enzyme catalytic reaction under extreme environments such as high temperature and low pH. The invention provides an enzyme preparation tool with excellent performance for efficient degradation of cellulose biomass, and has important application value.

To provide an imitation shrimp having a distinctive springy texture like that of natural shrimp.SOLUTION: An imitation shrimp comprises a matrix containing glucomannan and a polysaccharide (other than glucomannan) in which plant protein particles containing a seasoning and a colorant are dispersed.SELECTED DRAWING: Figure 1

The current disclosure provides binding polypeptides (e.g., antibodies), and effector moiety conjugates thereof (e.g., antibody-drug conjugates or ADCs), comprising a site-specifically engineered drug-glycan linkage within native or engineered glycans of the binding polypetpide. The current disclosure also provides nucleic acids encoding the antigen-binding polypeptides, recombinant expression vectors and host cells for making such antigen-binding polypeptides. Methods of using the antigen-binding polypeptides disclosed herein to treat disease are also provided.

The invention relates to a skin-cleaning composition and application thereof in cleaning cream, and belongs to the technical field of daily chemicals, the skin-cleaning composition comprises the following raw materials: plant fermentation liquor, a chrysanthemum artemisia extract, silk amino acid, cetyl alcohol ethyl hexanoate, PEG-20 glyceryl triisostearate and soapberry seed oil; the preparation method of the plant fermentation liquid comprises the following steps: S1, pretreating part of the raw materials; s2, activating and culturing the strain; and S3, adjusting the pH value of the mixed solution A, performing temperature-controlled sterilization, cooling, adding mannase, performing temperature-controlled enzymolysis, performing enzyme deactivation after enzymolysis is completed, so as to obtain a mixed solution B, cooling, adding a lactobacillus acidophilus fermentation seed solution and a streptococcus thermophilus fermentation seed solution, performing temperature-controlled standing fermentation, performing temperature-controlled sterilization and speed-controlled centrifugation after fermentation is completed, taking supernate, and performing 0.22 mu m membrane microfiltration, so as to obtain the plant fermentation liquor. The raw materials of the skin-cleaning composition provided by the invention have a synergistic effect, so that the skin-cleaning composition has the effects of moisturizing and whitening while effectively cleaning the skin.

The present disclosure relates to a low glycosylated spike protein and a vaccine designed to express the spike protein in vivo. The present disclosure also teaches a method for generating an immune response by utilizing the low glycosylated spike protein, which provides a broader protection across different variants. A method for identifying a glycan-shielded conserved peptide of a glycoprotein is also disclosed.

The invention discloses a radix pseudostellariae extract with high content of radix pseudostellariae cyclic peptide B and a preparation method and cosmetic application of the radix pseudostellariae extract, and belongs to the technical field of cosmetics. The preparation method comprises the following steps: (1) crushing radix pseudostellariae, adding alcohol and performing ultrasonic treatment to obtain feed liquid; (2) adding a compound enzyme into the feed liquid to carry out enzymolysis, enzyme deactivation, solid-liquid separation, concentration and impurity removal to obtain an enzymatic hydrolysate; and (3) eluting the enzymatic hydrolysate with macroporous resin to obtain an eluent, and drying to obtain the product, the compound enzyme is prepared from pectinase, papain, beta-glucosidase, cellulase and xylanase according to the mass ratio of (5 to 10) to (0.5 to 1) to (3 to 7) to (0.3 to 1). The radix pseudostellariae extract disclosed by the invention is prepared by adopting an enzymolysis process, more active ingredients can be reserved, the content of radix pseudostellariae cyclic peptide B in the obtained extract is high, and the radix pseudostellariae extract has relatively good anti-inflammatory, soothing, repairing and anti-aging effects.

The invention discloses an oral monoclonal antibody micro-coated nanopolysaccharide microsphere preparation for targeted therapy of inflammatory bowel diseases and a preparation method thereof. According to the method, oxidized mannan and an anti-TNF-alpha monoclonal antibody are mixed and cross-linked by a cross-linking agent containing a diselenide bond to form the drug-loaded nanoparticles. Mixing the drug-loaded nanoparticles with pectin, dropwise adding the mixture into a calcium chloride solution through an electrostatic spinning device, and carrying out ionic crosslinking to obtain the oral monoclonal antibody micro-coated nano polysaccharide microsphere preparation with the particle size range of 180-200 microns. The drug-loaded polysaccharide microsphere delivers the wrapped drug-loaded nanoparticles to a colitis disease part through electrostatic interaction. The drug-loaded nanoparticles are targeted to macrophages, release of the monoclonal antibody is accelerated under the triggering of high-concentration active oxygen at an inflammation part, and the oral stability and the treatment effect of the monoclonal antibody drug are improved. The polysaccharide is used as a monoclonal antibody targeted delivery carrier, raw materials are cheap and easy to obtain, the preparation process is simple and convenient, reaction conditions are mild, and application and development are easy.

The invention provides a soluble dietary fiber composition. The soluble dietary fiber composition comprises galactomannan, kelp dietary fibers, oat beta glucan, galactooligosaccharide, polydextrose, stachyose and xanthophyll. The invention also provides application of the soluble dietary fiber composition in functional food or constipation treatment medicines.

Provided herein are novel stabiligases for use in cell membrane proteome analysis. The subject stabiligases are capable of attaching to glycans found on the surface of cell membranes to form glycan-tethered (GT) stabiligases. Such glycan-tethered stabiligases are capable of robustly and selectively attaching label probes to cell surface proteins of intact cells. The subject novel stabiligases described herein advantageously allow for the identification and profiling of cell membrane proteins that have undergone an extracellular N-terminal proteolytic event.

The invention provides a Chinese date modified acidic polysaccharide. Each 100 mg of the Chinese date modified acidic polysaccharide contains 90.26 mg + / -1.39 mg to 94.42 mg + / -1.83 mg of total polysaccharide, the total uronic acid content is 21.42 mg + / -1.01 mg to 50.72 mg + / -0.62 mg; total bound phenol: 0.65 mg of GAE + / -0.05 mg of GAE-1.21 mg of GAE + / -0.03 mg of GAE (equivalent weight of gallic acid). The invention also provides a preparation method and application of the jujube modified acidic polysaccharide. The jujube acidic polysaccharide is modified through a low-temperature alkaline deesterification technology, a directional enzyme digestion technology and a controllable partial acid hydrolysis technology, the in-vivo and in-vitro immunomodulatory activity of the jujube acidic polysaccharide before and after modification is compared, and the immunomodulatory activity of the jujube modified acidic polysaccharide obtained through modification is remarkably enhanced; especially, the jujube modified acidic polysaccharide with araban side chains removed and galactan side chains reduced has better immunocompetence.

The invention relates to the field of extraction of sacha inchi oil, in particular to a preparation method and application of sacha inchi oil, and aims to solve the problems of large loss of heat-sensitive components, solvent residue and low oil yield in the traditional process. The ratio of cellulase to pectinase to xylanase to lipase is 3: 2: 1: 0.5, and carrying out enzymolysis for 2-3 hours at the pH value of 4.5-5.5 and the temperature of 45-50 DEG C; the obtained enzymolysis product is subjected to 28-40kHz ultrasonic treatment for 20-30 minutes, and then is subjected to two-stage gradient squeezing (pre-squeezing at 50-55 DEG C to final squeezing at 35-40 DEG C) in combination with 0.1 mu m ceramic membrane filtration, so that efficient oil extraction and active ingredient protection are realized. The oil yield of the sacha inchi oil is increased to 50.0%, the content of alpha-linolenic acid is larger than or equal to 42%, the content of vitamin E reaches 158 mg / 100 g, no solvent is left, and the sacha inchi oil is suitable for the fields of functional food and cosmetics.

The invention discloses chimeric xylanase XynZT-1-Z5 and a construction method thereof, and relates to the technical field of molecular biology, and the technical key points are as follows: comparing the sequence of xylanase XynZT-1 with the sequence of xylanase from different thermoduric bacteria, analyzing the amino acid conserved site of the xylanase XynZT-1, and replacing the sequence beta5-beta6 of the thermoduric bacteria onto the XynZT-1 to obtain the chimeric xylanase XynZT-1-Z5. The chimeric enzyme XynZT-1-Z5 has efficient overexpression, good solubility and relatively strong temperature and pH stability, is particularly suitable for industrial enzyme catalytic reaction under the conditions of high temperature and extreme pH, and can be widely applied to the food industry, the wine brewing industry, the papermaking industry and the feed industry.

The invention discloses an extracellular vesicle and a preparation method and application thereof, and belongs to the technical field of cosmetics, and the preparation method comprises the following steps: mixing rose and a PBS buffer solution, juicing, and filtering to obtain a filtrate; adding cellulase, xylanase and beta-glucanase into the filtrate, carrying out enzymolysis, and filtering to obtain an enzymatic hydrolysate; and centrifuging the enzymatic hydrolysate, filtering, resuspending and precipitating to obtain the rose extracellular vesicles. The rose extracellular vesicles can guarantee structural integrity and biological activity, can effectively load active ingredients, and can effectively deliver active molecules in the rose extracellular vesicles to target cells through interaction with membranes of skin cells. The extracellular vesicles can be fused with skin cell membranes to enter cells, active ingredients encapsulated by the extracellular vesicles are released, and the absorption efficiency of skin is improved.

The invention discloses a bi-crosslinking gelatin-based hydrogel wound dressing as well as a preparation method and application thereof, and belongs to the technical field of biomedical materials. The preparation method disclosed by the invention comprises the following steps: mixing a gelatin solution and an oxidized konjac glucomannan solution, heating and stirring to obtain a hydrogel precursor solution; and mixing the hydrogel precursor solution with a borax solution to obtain the bi-crosslinked gelatin-based hydrogel wound dressing. According to the method, oxidized konjac glucomannan and gelatin are subjected to Schiff base and Schiff base crosslinking, borax is further added to introduce a dynamic boric acid ester bond effect to enhance a three-dimensional network of the hydrogel, and the dynamic reversible Schiff base effect and the boric acid ester bond effect are enhanced, so that the pore diameter and the swelling ratio of the hydrogel are increased, and the gel strength of the hydrogel is enhanced; due to the good self-healing injectable performance, biodegradability and mechanical property of the hydrogel, the hydrogel can be used as a wound dressing for irregular deep skin wounds, and the technical problem that existing hydrogel is insufficient in treatment of irregular wounds is solved.

The preparation method comprises the following steps: degreasing polygonatum cyrtonema leaf superfine powder, sequentially carrying out ultrasonic-assisted enzymolysis treatment on cellulase, xylanase, high-temperature-resistant alpha-amylase, papain and saccharifying enzyme, and carrying out alcohol precipitation, dialysis and vacuum freeze drying treatment to obtain the polygonatum cyrtonema leaf polysaccharide. The polygonatum cyrtonema leaf enzyme extraction crude polysaccharide is obtained; and separating the enzyme-extracted crude polysaccharide through a cellulose column, and purifying through a sephadex column to obtain the polygonatum cyrtonema leaf enzyme-extracted polysaccharide. Polysaccharide in polygonatum cyrtonema leaves is extracted through ultrasonic-assisted compound enzyme, single pure polysaccharide is obtained through alcohol precipitation, dialysis, cellulose column chromatography and gel column chromatography, and the purity can reach 99.86%; the obtained enzyme extraction polysaccharide has good anti-cancer activity, can significantly inhibit proliferation of breast cancer cells and colorectal cancer cells, has the characteristics of good biocompatibility, strong specificity, low toxic and side effects and the like, and has wide application prospects.

The invention discloses cubilose sialic acid glycan peptide capable of improving lung inflammation and application of the cubilose sialic acid glycan peptide. The ratio of fucose modified sugar chains in the cubilose sialic acid glycan peptide is 30-60%, the ratio of sialic acid modified sugar chains in the cubilose sialic acid glycan peptide is 20-40%, the core structure of the fucose type is (HexNAc) 3 (Man) 3 (Fuc) 1, the core structure of the sialic acid type is (HexNAc) 3 (Man) 3 (Gal) 1 (Fuc) 1 (NeuAc) 1, the neutral sugar dissolution rate of the cubilose sialic acid glycan peptide is high, and the glycopeptide shown in SEQ ID NO 2 has a good inflammation improving effect.

The present invention relates to the use of a Bifidobacterium longum transitional microorganism to metabolise one or more glycan substrates selected from the group recited in any of Tables 1 to 3.

The invention relates to the technical field of dendrobium officinale polysaccharide processing and active ingredient function improvement, in particular to a preparation method of a dendrobium officinale polysaccharide fragment. According to the specific technical scheme, the method comprises the following steps: adding mannase with enzyme activity of 0.7-1U into a dendrobium officinale polysaccharide solution, and stirring for 0.5-1.5 hours; and adding absolute ethyl alcohol until the concentration is 65-75%, centrifuging to remove supernate, collecting precipitate, adding water to redissolve, concentrating, and freeze-drying to obtain the dendrobium officinale polysaccharide fragment. The dendrobium officinale polysaccharide with higher immunoregulatory activity can be conveniently and rapidly obtained in an enzymolysis mode.

Disclosed in the present invention is a process for preparing a monosaccharide solution by means of acid gradient treatment of a straw lignocellulose. The process comprises the steps of: S1: performing a first acidolysis reaction on a lignocellulose biomass using an acid solution with a concentration of 50 wt% or above to obtain a first acidolysis solution; S2: uniformly mixing the first acidolysis solution with an organic solvent, and performing solid-liquid separation to obtain a glycan-containing residue; and S3: adding water and / or an acid solution to the glycan-containing residue to obtain a mixed solution with an acid concentration of 45 wt% or below, and performing a second acidolysis reaction to obtain a monosaccharide-containing reaction solution. In the present invention, pretreatment is performed at room temperature and atmospheric pressure without the need to provide additional energy, and various agents used in the pretreatment process are all recoverable, which greatly reduces the production cost. In addition, in the present invention, a relatively low liquid-solid ratio is used, monosaccharide can be directly obtained after the pretreatment, and finally a monosaccharide solution with a high and adjustable concentration is obtained, thereby avoiding a large amount of input in enzyme costs.

Compositions containing a nucleic acid nanostructure having a desired geometric shape and an antigen and / or immunostimulatory agent(s) bound to its surface are provided. The nanostructure design allows for control of the relative position and / or stoichiometry of the immunostimulatory agent(s) bound to its surface. The antigen and / or immunostimulatory agent(s) displayed on the nanostructure surface are arranged with the preferred number, spacing, and 3D organization to elicit a robust immune response. The displayed antigen can be eOD-GT8. The immunostimulatory agent can be, e.g., T cell epitope such as a pan HLA DR-binding epitope (PADRE) and / or a lectin such as MBL or C3, or ligand thereof such as a glycan including mannose. Also provided are antigen-T cell epitope fusions such as eOD-PADRE and nanostructures presenting the same. The immunostimulatory compositions may thus be useful as immunogens, vaccines, adjuvants, and the like. Methods of inducing immune responses are also provided.

The invention relates to the technical field of functional food, in particular to immune regulation type prebiotic syrup with synergy of galactomannan. The active components of the composite solid beverage are prepared from the following raw materials in percentage by weight: 20%-35% of fructo-oligosaccharide, 10%-18% of galactooligosaccharide, 8%-15% of stachyose, 3%-7% of xylooligosaccharide, 5%-12% of lactitol, 5%-10% of galactomannan, 2%-5% of yeast beta-glucan and 1%-3% of N-acetylglucosamine. The composition further comprises 4-8% of inulin and 3-6% of resistant dextrin as a matrix filling and slow-release carrier, and the sum of the weight percentages of all the active ingredients and the carrier is 100%. By remodeling intestinal microecology and inhibiting inflammation pathways, the intestinal inflammation state can be remarkably improved, expression of intestinal mucosa tight junction protein is promoted, and an innovative solution is provided for people suffering from chronic bowel diseases and immune imbalance.

The invention provides a feed for relieving weaning stress of lambs and a preparation method of the feed, and belongs to the field of animal feeds. The feed disclosed by the invention is prepared from the following components in parts by weight: 35-40 parts of puffed corn, 15-20 parts of enzymolysis soybean meal, 5-8 parts of fish meal, 5-8 parts of alfalfa meal, 0.3-3 parts of an organic trace element compound, 0.1-2 parts of a vitamin compound, 3-5 parts of whey powder, 0.5-1 part of butyrin, 0.3-0.5 part of glutamine and 0.2-0.3 part of a radix astragali-spina date seed compound extract. 0.2 to 0.3 part of xylooligosaccharide-xylanase compound, 0.05 to 0.1 part of pancreatin active peptide and 0.1 to 2 parts of complex microbial inoculant. The weaning stress of the lambs is reduced through a targeted intestinal tract-immunity-nerve regulation ternary synergistic formula, the digestive system development of the weaned lambs can be obviously improved, the activity of digestive enzymes can be improved, and therefore the weight gain of the lambs is obviously improved, and the lambs stably pass through the weaning period.