288 results about "Mannose" patented technology

The invention discloses a macrophage-targeting inhalable nano material, a preparation method thereof and application of the inhalable nano material in treatment of sepsis myocarditis, and belongs to the technical field of medicines. The nano-material is a nano-liposome, the nano-liposome comprises a mannose modified nano-liposome microsphere, and the mannose modified nano-liposome microsphere comprises a liposome skeleton shell layer, a drug and a targeting layer; the liposome skeleton shell layer comprises soybean lecithin and cholesterol; the medicine comprises quercetin and TPPU (Thermoplastic Polyurethane); and the targeting layer is formed by connecting mannose modified DSPE-PEG2000 to the outer surface of the liposome skeleton shell layer. The nano material can accurately target macrophages at a cardiac inflammation part, has a multi-target-point synergistic effect, is more convenient and faster in administration, remarkably improves the safety and comprehensively improves the treatment effect.

The invention relates to the field of cosmetics, in particular to dendrobium devonianum stem extract as well as a preparation method and application thereof. The invention provides a dendrobium devonianum stem extract. The mass ratio of glucose to mannose in the dendrobium devonianum stem extract is 3: 7. The invention provides the dendrobium devonianum stem extract with tightening and moisturizing functions, and the extract can effectively inhibit ROS secreted by UVB-induced in-vitro keratinocytes at a lower concentration, and has a certain tightening effect; the AQP3 level in keratinocytes is improved, the AQP3 gene expression is up-regulated, and a certain moisturizing effect is achieved; the dendrobium devonianum stem extract is used for a formula human body efficacy test, and it is proved that 0.1% of the dendrobium devonianum stem extract has certain tightening and moisturizing effects.

Provided are pharmaceutical formulations comprising a recombinant acid α-glucosidase, wherein the recombinant acid α-glucosidase is expressed in Chinese hamster ovary (CHO) cells and comprises an increased content of N-glycan units bearing one or two mannose-6-phosphate residues when compared to a content of N-glycan units bearing one or two mannose-6-phosphate residues of alglucosidase alfa; at least one buffer selected from the group consisting of a citrate, a phosphate and combinations thereof; and at least one excipient selected from the group consisting of mannitol, polysorbate 80, and combinations thereof, wherein the formulation has a pH of from about 5.0 to about 7.0. Also provided are methods of treating Pompe disease using these pharmaceutical formulations.

The invention discloses a mannose modified liposome gel microsphere oral delivery system based on cations and application of the mannose modified liposome gel microsphere oral delivery system. The oral delivery system comprises a core layer and a wrapping layer, the core layer is mannose modified cationic liposome loaded with a mucous membrane adjuvant retinoic acid and an antigen, and the wrapping layer is sodium alginate gel vulcanized and modified by cysteine. The mannose modified cationic liposome is prepared by coupling mannose on the surface of the cationic liposome, and the cysteine modified sodium alginate gel is used for protecting antigens in the core layer from being eroded by gastric juice, enhancing the adhesiveness of a delivery system in the intestinal tract and prolonging the retention time of the delivery system in the intestinal tract; in addition, the R-MLip can be expanded and released under the environment that the pH of the intestinal tract is increased. The inside of the gel microsphere of the oral delivery system shows a regular net-shaped structure and has abundant holes and channels, and the oral delivery system shows a good prospect in the aspect of enhancing mucous membrane and systemic immunity and possibly becomes a potential substitute of a traditional attenuated live vaccine in the future.

The invention provides a series of GDP-mannose dehydratase polypeptides which can be applied to synthesis of GDP-fucose, and belongs to the technical field of enzyme engineering. The GDP-mannose dehydratase polypeptide comprises a polypeptide of which the amino acid sequence is at least 70%, 75%, 80%, 85%, 90%, 95%, 99% and 100% identical to that of a polypeptide shown in SEQ ID NO: 1, or a polypeptide of which the amino acid sequence is at least 70%, 75%, 80%, 85%, 90%, 95%, 99% and 100% identical to that of a polypeptide M1-13. The GDP-mannose dehydratase polypeptide disclosed by the invention has relatively high catalytic activity.

The application discloses a Dendrobium nobile Lindl polysaccharide component group, a preparation method thereof and application of the Dendrobium nobile Lindl polysaccharide component group in immunoregulation. Four polysaccharide components with definite structures, namely DNP-0-1 (>100 kDa), DNP-0-2 (30-100 kDa), DNP-0-3 (<30 kDa) and DNP-1-1 (<50 kDa), are synchronously separated from Dendrobium nobile Lindl by combining ultrasonic extraction process optimized by a response surface method with DEAE-cellulose column chromatography and ultrafiltration membrane series grading technology. After structure characterization and in-vitro immunological activity evaluation, the four components all have immunoregulatory activity, and the activity of DNP-0-1 is optimal. Pearson correlation analysis shows that the content of galactose and the molecular weight are extremely significantly positively correlated with the immunological activity, and the content of glucose and mannose is significantly negatively correlated with the activity. The application establishes an integrated research system of 'preparation by grading-structure characterization-activity screening-structure-activity analysis', and provides a material basis and evaluation basis for the development of immunoregulatory drugs or functional foods.

This invention relates to a method for preparing dapavancin precursor A40926, the method comprising adding mannose to the fermentation broth during fermentation. The preparation method of this invention can significantly reduce the content of impurity ZZ1 in the fermentation product without increasing other unknown impurities; A0, A1, B0, B1, and B2 all meet the dapavancin quality standards; simultaneously, the process is simple, the production cost is low, and it is suitable for large-scale industrial production.

The application provides probiotic oral burst balls and a preparation process, and belongs to the technical field of probiotic embedding. The probiotic oral burst balls comprise core materials and wall materials. The core materials comprise probiotic raw materials, a flavoring agent, mannose, xylitol, mannose oligosaccharide, glycine, vitamin E and a solvent 1, and the solvent 1 is an oily solvent. The wall materials comprise trehalose, gelatin, cyclic dextrin and a solvent 2, and the solvent 2 is an oily solvent. The probiotic oral burst balls prepared by the preparation process have the characteristics of temperature resistance, acid resistance and storage resistance.

The invention relates to the technical field of agricultural chemistry, and discloses a method for enhancing leaf surface wetting spreading and prevention effects of a herbicide by using mannosylerythritol lipid. Comprising the steps of S1, material and equipment preparation, S2, MELs synergist formula optimization, S3, herbicide and MELs synergist compounding, S4, standardized pesticide application process step, S5, effect verification and evaluation step and S6, cost optimization and matters needing attention. The MELs synergist adopted in the method has excellent interfacial activity, the 60s contact angle on hydrophobic wheat leaves is smaller than or equal to 36 degrees under the concentration of 2%, the hydrophilic crop leaf surface contact angle is smaller than or equal to 34 degrees, the canvas sedimentation time is as low as 6.75 s and is far better than that of a traditional auxiliary agent Tween 80, through a standardized pesticide application process, the pesticide liquid can be spontaneously and evenly spread on the hydrophilic and hydrophobic leaf surfaces, and the pesticide liquid adhesion rate is remarkably increased. Compared with a traditional method, the leaf surface adhesion rate is increased by 50% or above, and the problem of liquid medicine loss and waste is effectively solved.

The invention discloses armillariella mycelium polysaccharide ATMP capable of improving cognitive impairment and a preparation method and application of the armillariella mycelium polysaccharide ATMP. The polysaccharide is composed of two components, and the molecular weights of the two components are 2.30 * 10 < 4 > Da and 4.56 * 10 < 5 > Da respectively; the monosaccharide composition comprises mannose (Man), glucose (Glc) and galactose (Gal), and the molar ratio of the mannose (Man) to the glucose (Glc) to the galactose (Gal) is 1: 16.64: 1.96. Animal experiment results show that the armillariella mycelium polysaccharide ATMP can improve cognitive impairment induced by lipopolysaccharide (LPS) by improving the spatial memory ability of mice, repairing the disorder state and deletion injury of neurons in a hippocampal region and relieving neuroinflammatory response, so that the obvious neuroprotective effect is achieved. As a natural brain-benefiting active component, the armillariella tabescens mycelium polysaccharide ATMP has a wide application prospect in the related fields of brain health.

The application belongs to the technical field of medicines, and discloses a mannose-modified nano silver cluster, a preparation method and application of the mannose-modified nano silver cluster as a probe, wherein the mannose-modified nano silver cluster is obtained by coupling bovine serum albumin-nano silver clusters and modified mannose through acid-amine condensation reaction, 14 modified mannose molecules are coupled on each bovine serum albumin molecule, the mannose-modified nano silver cluster can be applied as a probe in biological recognition and target labeling, a new visual tool is provided for receptor-ligand interaction research and drug delivery monitoring, and the application development of the sugar probe in biomedical detection and diagnosis and treatment is promoted.

Device (1) and system for macrofiltration are disclosed. The macrofiltration comprises mannosylerythritol lipids (MELs) recovery, the MELs being in the form of particles. System comprising the device (1) is also disclosed. The system comprises a first pathway (6) in fluid communication, wherein this first pathway comprises sequentially a first valve (3), a first pump (4), a bioreactor (2) and a second valve (10), and where this first pathway is between the at least one outlet and inlet of the device (1). Process for enhancing mannosylerythritol lipids (MELs) production is also disclosed.

The invention provides yellow-rose yellow-meat boletus fucomannan as well as a composition, a preparation method and application thereof, and belongs to the technical field of biological medicine mass health. The fucomannan has a structure represented by a general formula in an attached drawing of the abstract, and n represents the number of sugar repeating units and is a natural number. The weight-average molecular weight of the fucose glucomannan galactan of the boletus fucose is 10 kDa to 20 kDa, the polydispersity coefficient of the fucose glucomannan galactan is 1 to 2, and the molar ratio of glucose to mannose to fucose to galactose is 1.00 to 3.08 to 2.84 to 14.86. The boletus fucomannan and the composition containing the boletus fucomannan and the composition containing the boletus fucomannan and the composition containing the boletus fucomannan have the effects of promoting proliferation of beneficial bacteria, reducing the pH value of a microenvironment, increasing secretion of SCFAs and lactic acid, adjusting composition of intestinal microorganisms and the like, and have the function of improving the intestinal microecology. Therefore, the invention also discloses the fucomannan-glucomannan of the boletus roseus and the fucomannan-glucomannan of the boletus roseus and the fucomannan-glucomannan of the boletus roseus and the fucomannan-glucomannan of the boletus roseus and the fucomannan of the composition of the boletus roseus and the fucomannan of the composition of the boletus roseus and the fucomannan of the composition of the boletus roseus and the fucomannan of the composition of the composition of the composition of the composition of the composition of the composition of the composition of

A composition comprises: a) at least one cellulose-based polymer; b) at least one amphoteric surfactant; and c) at least one monosaccharide chosen from mannose, rhamnose, and mixtures thereof. A process for and / or removing makeups from keratin materials, comprises applying the composition to the keratin materials and rinsing off said composition after an optional period of time.

The application provides a yeast mannose oligosaccharide and a preparation method and application thereof. The preparation method comprises the following steps: S1, mixing yeast cell walls with water to obtain a mixed solution; S2, adjusting the pH of the mixed solution to 4-9, performing first enzymolysis on the mixed solution by using a protease to form a first enzymolysis product; secondly, performing second enzymolysis on the first enzymolysis product by using a polysaccharide enzyme to obtain a second enzymolysis product; the amount of the protease is greater than or equal to 0.2% of the weight of the yeast cell walls, and the amount of the polysaccharide enzyme is greater than or equal to 0.1% of the weight of the yeast cell walls; S3, centrifuging the second enzymolysis product to obtain a supernatant and a centrifugal heavy phase; filtering the supernatant by using an ultrafiltration membrane, and the retentate is the yeast mannose oligosaccharide, the permeate is a yeast extract, and the centrifugal heavy phase is a yeast glucan. The yeast mannose oligosaccharide obtained by the application has high purity and high yield, the mannose oligosaccharide is clean, can be fermented by beneficial bacteria, and can be applied as a prebiotic in food.

The application provides a copper selenide-halloysite-mannose composite drug delivery system, which comprises a carrier, the carrier is halloysite nanotubes (HNTs), copper selenide nanoparticles (CuSe) are in situ grown on the tube wall of the halloysite nanotubes to form a composite CuSe@HNTs; and mannose is loaded in the lumen of the CuSe@HNTs to form a composite drug delivery system CuSe@HNTs@ME. The application also provides a preparation method and application of the copper selenide-halloysite-mannose composite drug delivery system. The drug delivery system can maintain stability in a gastric acid environment, directly reaches an intestinal inflammation site through an oral route, and significantly reduces intestinal inflammation symptoms.

The application discloses Ixeris sonchifolia Hance polysaccharides, a preparation method and application thereof, belongs to the technical field of plant polysaccharides, and provides polysaccharides ISP-1a and ISP-2a, wherein the ISP-1a has a molecular weight of 2086 Da and is composed of seven monosaccharides of mannose, rhamnose, glucuronic acid, glucose, galactose, xylose and arabinose; the ISP-2a has a molecular weight of 1781 Da and is composed of five monosaccharides of mannose, rhamnose, galacturonic acid, xylose and arabinose. The whole grass of Ixeris sonchifolia Hance is subjected to hot water extraction after ethanol degreasing, neutral enzyme + Sevage method is used to remove protein, macroporous resin is used for decolorization, column chromatography elution is carried out, purification is carried out, and two kinds of uniform polysaccharides are obtained after freeze-drying. The polysaccharides ISP-1a and ISP-2a have good antioxidant and lipid-lowering effects, and can be applied to the preparation of food, health products or medicines with antioxidant and lipid-lowering functions.

The invention discloses a method and a medicament for relieving tobacco root rot and repairing a root system. A tobacco root rot relieving and root system repairing method comprises the following steps that S1, in the first-aid induction stage, 3-5 days after transplanting, induction liquid is applied in a irrigating mode, and the induction liquid comprises chitosan oligosaccharide, betaine, nano SiO2 and ZnSO4; s2, a slow-release protection stage: 3-7 days after irrigation, chitosan and gelatin coated controlled-release particles are applied to the tobacco root zone, the controlled-release particles comprise mannitol, water-soluble zinc and potassium humate, and the pH value of the root zone is maintained to be 6.0-6.8; s3, an ecological maintenance stage, the period is 15 days, a nutrient solution is applied, and the nutrient solution comprises organic acid, amino acid calcium and magnesium nitrate. The invention provides a green scheme without chemical pesticides and exogenous strains, and the control effect of the root rot is not less than 70%; the root system damage repair time is shortened; the root zone microenvironment is continuously regulated for more than or equal to 15 days.

This disclosure provides one or more oligomannose-type N-glycans and glycosylated Fc domain variants containing Fc domain mutations. The disclosure also provides nucleic acids encoding Fc domain variants and host cells for constructing Fc domain variants. Methods for increasing the yield of Fc domain variants and methods for treating diseases using Fc domain variants are also provided.

The invention belongs to the technical field of cosmetics, and particularly discloses mannosylerythritol lipid (MELs) serving as a PPAR alpha agonist and application of the MELs in cosmetics. The MELs is a single component, and can significantly activate keratinocyte PPARalpha and a downstream pathway thereof, promote synthesis of glucosylceramide and hyaluronic acid, up-regulate expression of barrier related proteins (OCLN, CLDN1, IVL, TGM1 and FLG) and aquaporin AQP3, inhibit inflammatory factors IL-1beta and TNF-alpha, and realize endogenous skin barrier repair. According to the invention, MELs is used as a PPAR alpha agonist for cosmetics for the first time, and a new raw material is provided for skin barrier repair.

The method for catalytically preparing D-mannose provided in the application can effectively reduce the amount of by-products glucose and fructose, and greatly improve the conversion rate of D-mannose. Specifically, the engineering bacteria expressing isoamylase gene and the engineering bacteria expressing alpha-glucan phosphorylase gene, glucose phosphomutase gene, bifunctional enzyme glucose phosphate isomerase / mannose 6-phosphate isomerase gene and mannose 6-phosphate phosphatase gene are used, starch or starch derivatives are used as substrates, phosphate buffer solution and Mg 2+ A preliminary catalytic system is constructed, and after the reaction is completed, there are unreacted substrates and maltodextrin in the reaction system, the saccharifying enzyme can hydrolyze the unreacted substrates and maltodextrin into glucose, the polyphosphate glucose kinase can convert all the glucose into phosphorylated glucose with the assistance of sodium hexametaphosphate, and then D-mannose is generated, thereby improving the yield and removing the by-products in the system.

The application discloses radix sophorae tonkinensis polysaccharide STGP-1, a preparation method thereof and pharmaceutical use for treating lung squamous carcinoma, and relates to the technical field of medicines. The monosaccharide composition of STGP-1 includes fucose Fuc, arabinose Ara, galactose Gal, glucose Glc, xylose Xyl and mannose Man, and the molar ratio is 0.34:12.15:25.76:57.07:1.06:3.62. The STGP-1 is obtained by extraction, separation and purification from dried roots and rhizomes of radix sophorae tonkinensis, and the preparation method comprises the steps of pretreatment and crude extraction, impurity removal treatment, anion exchange column chromatography separation, gel column chromatography purification and the like. The STGP-1 can be used alone or in combination with anti-PD-1 monoclonal antibody for preparing a medicine for treating lung squamous carcinoma. The preparation process is stable, the source is natural, and the STGP-1 can provide a new high-efficiency and low-toxicity candidate medicine for immune combined treatment of lung squamous carcinoma.

The invention discloses marsdenia tenacissima herb residue polysaccharide as well as a preparation method and application thereof. The invention discloses marsdenia tenacissima herb residue polysaccharide, the marsdenia tenacissima herb residue polysaccharide comprises TGT-1 or TGT-2, the average molecular weight of the TGT-1 is 19.929 kDa, the TGT-1 is composed of mannose, rhamnose, fucose, glucose and galactose, and the molar ratio of the mannose to the rhamnose to the fucose to the glucose to the galactose is 1: 1.11: 0.57: 22.62: 3.96; the average molecular weight of the TGT-2 is 11.762 kDa, the TGT-2 is composed of mannose, fucose, glucose and galactose, and the molar ratio of the mannose to the fucose to the glucose to the galactose is 2.01: 1: 35.21: 1.55. The polysaccharide components TGT-1 and TGT-2 are separated out, and the marsdenia tenacissima herb residue polysaccharide nano-selenium compound is prepared and has oxidation resistance and antitumor activity.

To provide a functional composition reduced in sulfur dioxide in a composition containing mannose and / or mannooligosaccharide.SOLUTION: The sulfur dioxide concentration in the functional composition can be reduced by decomposing devil's tongue powder derived from devil's tongue as a raw material and then heat-treating the resultant composition containing mannose and / or mannooligosaccharide at ≥ 105 °C. The functional composition with reduced sulfur dioxide (sulfurous acid) can be distributed as food and drink without legal problems.SELECTED DRAWING: None

The invention discloses asterias amurensis glucan as well as a preparation method and application thereof, and belongs to the technical field of biological medicine, the asterias amurensis glucan is obtained by combining an enzymolysis method with anion exchange column chromatography and gel column chromatography purification, the main chain structure of the asterias amurensis glucan is 1, 4-linked alpha-D-glucose, and the main chain structure of the asterias amurensis glucan is 1, 4-linked alpha-D-glucose. A terminal alpha-D-glucose branch chain is arranged at the O-6 position of a main chain, and meanwhile, a small amount of mannose and galactose are contained. Moreover, it is proved for the first time that the asterias amurensis glucan can serve as an intestinal barrier protective agent, and oxidative damage, caused by H2O2, of intestinal epithelial cells is improved by up-regulating an Nrf2 signal channel and tight junction protein. Therefore, the asterias amurensis glucan prepared by the invention has a huge potential of being developed into medicines and health-care products for protecting intestinal tracts.

The invention relates to freshening and whitening toothpaste and a preparation method thereof. The freshening and whitening toothpaste comprises the following raw materials: hydrated silica, disodium dihydrogen pyrophosphate, a PVM / MA copolymer, color repair particles and an auxiliary agent, the color repair particles are prepared from mannitol, microcrystalline cellulose, CI 74160, cetyl alcohol, corn starch, ethyl cellulose and hydroxypropyl methyl cellulose. Through combination of triple whitening methods, tooth whitening is realized, a color locking film is formed on the surface of the whitened tooth, color spots and color spots are prevented, and the tooth yellowing risk is reduced. Experiments prove that the freshening and whitening toothpaste disclosed by the invention has an effect of freshening breath, the temperature of the inner wall of the oral cavity can be remarkably reduced by 4.5 DEG C by using the toothpaste, and the toothpaste has no irritation to the mucous membrane of the oral cavity and is high in safety.

Disclosed herein are C-mannoside compounds, compositions thereof and their application as pharmaceuticals for the treatment of human disease. Methods of inhibition of FimH activity in a human subject are also provided for the treatment of diseases such as bacterial infection, Crohn's disease (CD) and Inflammatory Bowel Disease (IBD).

Disclosed is a homogeneous polysaccharide, monosaccharide components of the homogeneous polysaccharide including mannose and glucose; wherein the main chain structure of the homogeneous polysaccharide is an alpha-(1→4)-linked glucose pyranose main chain; wherein the average molecular weight of the homogeneous polysaccharide is 11.1 ± 5.0 kDa. The homogeneous polysaccharide of the present application can be used for preventing and treating chronic liver disease.

The present application belongs to the technical field of application of fungal polysaccharides, and particularly relates to a Boletus edulis fruiting body polysaccharide as well as a preparation method and application thereof. Polysaccharides in Boletus edulis fruiting bodies are extracted, and the chemical structure of BDP-F is characterized by ultraviolet-visible light, Fourier transform infrared spectroscopy, one-dimensional nuclear magnetic resonance and two-dimensional nuclear magnetic resonance. The research results show that BDP-F is mainly composed of mannose, glucose, galactose and fucose. Methylation and nuclear magnetic resonance analysis show that the glycosidic bond of BDP-F is (1→6)-alpha-d-galactose, the main chain is composed of ->6)-alpha-d-galp-(1→ residues, and the O-2 position is branched by alpha-L-Fucp-(1→ and alpha-D-Manp-(1→ residues. Through DPPH free radical, hydroxyl radical and superoxide anion radical scavenging experiments, it is shown that BDP-F has the ability to serve as a natural antioxidant, and BDP-F can also reduce alcohol-induced GES-1 cell damage through antioxidant and anti-inflammatory effects within the experimental concentration range.

The invention discloses lake sphingosine bacteria AT4-200, exopolysaccharides thereof and application of the lake sphingosine bacteria AT4-200 in promoting plant growth and improving plant stress resistance, relates to the technical field of microbial technology and biostimulant application, and discloses the lake sphingosine bacteria AT4-200 separated and purified from tobacco rhizosphere and the exopolysaccharides generated by the lake sphingosine bacteria AT4-200, the molecular weight of the exopolysaccharides is about 1340Da, and the exopolysaccharides can be applied to plant growth promotion and plant stress resistance improvement. The polysaccharide mainly comprises five monosaccharides, namely mannose, glucose, glucosamine, galactosamine and rhamnose. Application verifies that the strain and / or exopolysaccharide thereof can significantly increase agronomic characters such as leaf area and dry weight of tobacco, can effectively improve salt tolerance of soybean under salt stress, and can be used as a novel, low-cost and efficient plant biostimulant or stress-resistant preparation.