471 results about "Mannose" patented technology

The invention belongs to the field of agricultural chemistry and bio-fertilizer, and discloses a fertilizer synergist based on polyaspartic acid, alginate oligosaccharide and mannan oligosaccharide and a preparation method of the fertilizer synergist. According to the synergist, polyaspartic acid derived from plant source amino acid, alginate oligosaccharide extracted from natural brown algae and mannan oligosaccharide extracted from plants are used as main raw materials, and the polyaspartic acid, the alginate oligosaccharide and the mannan oligosaccharide cooperate to form an induction-complexation-adsorption three-effect-in-one mechanism, wherein the alginate oligosaccharide and the mannan oligosaccharide induce plant root activity and stress resistance gene expression; the polyaspartic acid is dominated to form a multi-point weak complex network, so that slow release and controlled release of nitrogen and potassium ions are realized; the three components cooperate on the surface of soil colloid to form an adsorption layer, and nutrient leaching loss is reduced. The preparation process adopts a green process of'enzymatic pretreatment-segmented complexing-low-temperature forming ', so that the oligosaccharide activity is maintained, and the use of an organic solvent is avoided. The obtained synergist can significantly improve the nitrogen utilization rate and the root activity and prolong the fertilizer efficiency period, and is suitable for green and efficient agricultural production of various crops.

The invention discloses a macrophage-targeting inhalable nano material, a preparation method thereof and application of the inhalable nano material in treatment of sepsis myocarditis, and belongs to the technical field of medicines. The nano-material is a nano-liposome, the nano-liposome comprises a mannose modified nano-liposome microsphere, and the mannose modified nano-liposome microsphere comprises a liposome skeleton shell layer, a drug and a targeting layer; the liposome skeleton shell layer comprises soybean lecithin and cholesterol; the medicine comprises quercetin and TPPU (Thermoplastic Polyurethane); and the targeting layer is formed by connecting mannose modified DSPE-PEG2000 to the outer surface of the liposome skeleton shell layer. The nano material can accurately target macrophages at a cardiac inflammation part, has a multi-target-point synergistic effect, is more convenient and faster in administration, remarkably improves the safety and comprehensively improves the treatment effect.

The invention discloses a production method of fucosylated oligosaccharide. The core of the method is as follows: the GDP-D-rhamnose is converted into the GDP-L-fucose by using the GDP-D-rhamnose-3, 5-epimerase; the GDP-D-rhamnose is obtained by converting the GDP-D-mannose-4, 6-dehydratase and the GDP-4-keto-6-deoxy D-mannose reductase by taking the GDP-D-mannose as a substrate, and the GDP-D-rhamnose is obtained by taking the GDP-D-mannose as a substrate. The invention relates to a method for producing a fucosylated oligosaccharide, which is characterized in that a GDP-D-mannose-4, 6-dehydratase, a GDP-4-keto-6-deoxyD-mannose reductase, a GDP-D-rhamnose-3, 5-epimerase and a fucosyltransferase are expressed in a genetically modified host cell, and the fucosylated oligosaccharide can be produced by the way mentioned above.

The invention belongs to the technical field of preparation of probiotics, and provides a bacterial strain with anti-allergic and immunity-improving effects and application of the bacterial strain. According to the method, a three-layer physical self-assembly structure is adopted, and polysaccharide-polyphenol layer-by-layer self-assembly coating treatment, lipid insertion layer treatment and calcium ion crosslinking protection coating treatment are sequentially carried out. And mannan oligosaccharide MOS and / or galactooligosaccharide GOS are / is mixed in the chitosan layer in a ratio of 0.05-0.15% w / v, and the surface sugar ligand density reaches 4-8 [mu] g per 10 CFU. The coating is constructed under the condition that the pH value is 5.4-5.8; the thickness of the lipid layer is 20-80 nm when the pH value is 5.6-6.2; the cross-linked layer is treated at the pH of 6.0-6.5, so that the gastric acid environment is stable for 2 hours, and the gastric acid is controllably released in intestinal juice for 2-4 hours. The prepared strain has the total thickness of 220-900 nm, the outermost layer coverage rate is larger than or equal to 85%, the gastric juice survival rate is remarkably improved, the strain can be prepared into an oral preparation to be used for preventing and relieving allergic diseases, the viable count of each dose is 1 * 10 <-1 > * 10 <-1 > CFU, and the process is mild and suitable for industrial production.

Compositions containing a nucleic acid nanostructure having a desired geometric shape and an antigen and / or immunostimulatory agent(s) bound to its surface are provided. The nanostructure design allows for control of the relative position and / or stoichiometry of the immunostimulatory agent(s) bound to its surface. The antigen and / or immunostimulatory agent(s) displayed on the nanostructure surface are arranged with the preferred number, spacing, and 3D organization to elicit a robust immune response. The displayed antigen can be eOD-GT8. The immunostimulatory agent can be, e.g., T cell epitope such as a pan HLA DR-binding epitope (PADRE) and / or a lectin such as MBL or C3, or ligand thereof such as a glycan including mannose. Also provided are antigen-T cell epitope fusions such as eOD-PADRE and nanostructures presenting the same. The immunostimulatory compositions may thus be useful as immunogens, vaccines, adjuvants, and the like. Methods of inducing immune responses are also provided.

The invention discloses an emulsifier for preparing a Pickering emulsion, the Pickering emulsion as well as a preparation method and application of the Pickering emulsion. The emulsifier of the Pickering emulsion is obtained by dissolving distearoyl phosphatidylcholine, cholesterol and distearoyl phosphatidyl ethanolamine-polyethylene glycol 2000 in ethanol, carrying out rotary evaporation to remove ethanol, then adding an antigen aqueous solution containing an antigen, continuing rotary evaporation to obtain liposome nanoparticles, heating the liposome nanoparticles with mannose in a water bath, and carrying out freeze drying. The Pickering emulsion is obtained by dispersing an emulsifier in water as a water phase, taking squalene as an oil phase, mixing the water phase and the oil phase, and homogenizing. The Pickering emulsion disclosed by the invention is prepared by taking antigen presenting cell targeted liposome nanoparticles as a raw material; and the antigen has excellent ion concentration characteristic, pH stability, temperature stability and storage stability, and can protect the integrity of the antigen.

The invention relates to the field of cosmetics, in particular to dendrobium devonianum stem extract as well as a preparation method and application thereof. The invention provides a dendrobium devonianum stem extract. The mass ratio of glucose to mannose in the dendrobium devonianum stem extract is 3: 7. The invention provides the dendrobium devonianum stem extract with tightening and moisturizing functions, and the extract can effectively inhibit ROS secreted by UVB-induced in-vitro keratinocytes at a lower concentration, and has a certain tightening effect; the AQP3 level in keratinocytes is improved, the AQP3 gene expression is up-regulated, and a certain moisturizing effect is achieved; the dendrobium devonianum stem extract is used for a formula human body efficacy test, and it is proved that 0.1% of the dendrobium devonianum stem extract has certain tightening and moisturizing effects.

Provided are pharmaceutical formulations comprising a recombinant acid α-glucosidase, wherein the recombinant acid α-glucosidase is expressed in Chinese hamster ovary (CHO) cells and comprises an increased content of N-glycan units bearing one or two mannose-6-phosphate residues when compared to a content of N-glycan units bearing one or two mannose-6-phosphate residues of alglucosidase alfa; at least one buffer selected from the group consisting of a citrate, a phosphate and combinations thereof; and at least one excipient selected from the group consisting of mannitol, polysorbate 80, and combinations thereof, wherein the formulation has a pH of from about 5.0 to about 7.0. Also provided are methods of treating Pompe disease using these pharmaceutical formulations.

The invention belongs to the technical field of mesenchymal stem cell exosome compositions, and discloses a preparation method and application of a mesenchymal stem cell exosome composition. The mesenchymal stem cell exosome composition is prepared from a mesenchymal stem cell exosome, a hyaluronic acid-arginine grafted copolymer, acetyl tetrapeptide-5, resveratrol, mannose, polyglycerol-8 octoate and a buffer solution. Arginine residues contained in the hyaluronic acid-arginine graft copolymer in the composition can be specifically combined with integrin receptors (such as alpha v beta 3) highly expressed by fibroblasts at the wrinkle part, so that the exosome can actively anchor the wrinkle part, can be accurately and directionally enriched at the wrinkle part, and is high in utilization rate and high in anti-wrinkle efficiency. The composition constructs a three-dimensional anti-wrinkle network of mesenchymal stem cell exosome, acetyl tetrapeptide-5 and resveratrol, skin collagen degradation and oxidative damage are effectively restrained, and generation of skin wrinkles is blocked through multiple channels.

The invention discloses a mannose modified liposome gel microsphere oral delivery system based on cations and application of the mannose modified liposome gel microsphere oral delivery system. The oral delivery system comprises a core layer and a wrapping layer, the core layer is mannose modified cationic liposome loaded with a mucous membrane adjuvant retinoic acid and an antigen, and the wrapping layer is sodium alginate gel vulcanized and modified by cysteine. The mannose modified cationic liposome is prepared by coupling mannose on the surface of the cationic liposome, and the cysteine modified sodium alginate gel is used for protecting antigens in the core layer from being eroded by gastric juice, enhancing the adhesiveness of a delivery system in the intestinal tract and prolonging the retention time of the delivery system in the intestinal tract; in addition, the R-MLip can be expanded and released under the environment that the pH of the intestinal tract is increased. The inside of the gel microsphere of the oral delivery system shows a regular net-shaped structure and has abundant holes and channels, and the oral delivery system shows a good prospect in the aspect of enhancing mucous membrane and systemic immunity and possibly becomes a potential substitute of a traditional attenuated live vaccine in the future.

The invention provides pantoprazole sodium for injection, which is prepared by dissolving 40 parts of pantoprazole sodium, 25 parts of amino-PEG-mannose, 0.5 part of disodium calcium ethylene diamine tetraacetate and water for injection to 1000 parts and freeze-drying, the redissolution time of pantoprazole sodium for injection is short, related substances in the solution are not increased, the content is not obviously reduced, and the pantoprazole sodium for injection is suitable for clinical application. The stability is good in the transportation and storage process, the compatible solution can be stored for a long time during clinical use, clinical use becomes more convenient, and the problems of medication potential safety hazards, side effects and the like brought to patients due to impurity increase, content reduction and the like are also greatly reduced.

The invention provides a series of GDP-mannose dehydratase polypeptides which can be applied to synthesis of GDP-fucose, and belongs to the technical field of enzyme engineering. The GDP-mannose dehydratase polypeptide comprises a polypeptide of which the amino acid sequence is at least 70%, 75%, 80%, 85%, 90%, 95%, 99% and 100% identical to that of a polypeptide shown in SEQ ID NO: 1, or a polypeptide of which the amino acid sequence is at least 70%, 75%, 80%, 85%, 90%, 95%, 99% and 100% identical to that of a polypeptide M1-13. The GDP-mannose dehydratase polypeptide disclosed by the invention has relatively high catalytic activity.

The invention belongs to the technical field of medicine and health care, and discloses oleander oligosaccharide and a preparation method and application thereof, the molecular weight of the oleander oligosaccharide is 2900 Da, and the oleander oligosaccharide is composed of glucose, mannose, galactose and arabinose; the preparation method comprises the following steps: degreasing oleander roots with ethanol, respectively carrying out water extraction and gradient concentration alcohol extraction, and then sequentially carrying out ceramic membrane-ultrafiltration-nanofiltration enrichment, ion exchange column chromatography and molecular sieve gel column purification on the alcohol extract to obtain the oleander extract. The oligosaccharide can be applied to preparation of immunological enhancement drugs, corresponding tumor immune drugs or health care products. The preparation method is efficient and safe, and the prepared product is good in quality and can be applied to preparation of immunological enhancement drugs, corresponding tumor immune drugs or health care products.

The application discloses a Dendrobium nobile Lindl polysaccharide component group, a preparation method thereof and application of the Dendrobium nobile Lindl polysaccharide component group in immunoregulation. Four polysaccharide components with definite structures, namely DNP-0-1 (>100 kDa), DNP-0-2 (30-100 kDa), DNP-0-3 (<30 kDa) and DNP-1-1 (<50 kDa), are synchronously separated from Dendrobium nobile Lindl by combining ultrasonic extraction process optimized by a response surface method with DEAE-cellulose column chromatography and ultrafiltration membrane series grading technology. After structure characterization and in-vitro immunological activity evaluation, the four components all have immunoregulatory activity, and the activity of DNP-0-1 is optimal. Pearson correlation analysis shows that the content of galactose and the molecular weight are extremely significantly positively correlated with the immunological activity, and the content of glucose and mannose is significantly negatively correlated with the activity. The application establishes an integrated research system of 'preparation by grading-structure characterization-activity screening-structure-activity analysis', and provides a material basis and evaluation basis for the development of immunoregulatory drugs or functional foods.

This invention relates to a method for preparing dapavancin precursor A40926, the method comprising adding mannose to the fermentation broth during fermentation. The preparation method of this invention can significantly reduce the content of impurity ZZ1 in the fermentation product without increasing other unknown impurities; A0, A1, B0, B1, and B2 all meet the dapavancin quality standards; simultaneously, the process is simple, the production cost is low, and it is suitable for large-scale industrial production.

This invention belongs to the field of glycopolymer technology, and particularly relates to a tetraphenylethylene mono-arm thiomannose polymer with a side chain, its preparation method, and its application. The chemical structural formula of the tetraphenylethylene mono-arm thiomannose polymer is shown in formula (I). Wherein, n is an integer ≥2. The glycopolymer of this invention exhibits high biocompatibility and fluorescent recognition properties, and innovatively utilizes efficient and rapid RAFT polymerization and click chemistry to prepare the tetraphenylethylene mono-arm thiomannose polymer.

The invention belongs to the technical field of sugar synthesis chemistry, and particularly relates to a preparation method of L-mannose. According to the method, 1, 2, 3, 4, 6-penta-O-acetyl-beta-L-glucopyranose is used as a raw material, L-mannose is obtained by modifying and overturning L-glucose 2-OH, the raw material is easy to obtain, and the method is simple in process, low in cost, high in yield and suitable for large-scale production.

The application provides probiotic oral burst balls and a preparation process, and belongs to the technical field of probiotic embedding. The probiotic oral burst balls comprise core materials and wall materials. The core materials comprise probiotic raw materials, a flavoring agent, mannose, xylitol, mannose oligosaccharide, glycine, vitamin E and a solvent 1, and the solvent 1 is an oily solvent. The wall materials comprise trehalose, gelatin, cyclic dextrin and a solvent 2, and the solvent 2 is an oily solvent. The probiotic oral burst balls prepared by the preparation process have the characteristics of temperature resistance, acid resistance and storage resistance.

The invention relates to the technical field of agricultural chemistry, and discloses a method for enhancing leaf surface wetting spreading and prevention effects of a herbicide by using mannosylerythritol lipid. Comprising the steps of S1, material and equipment preparation, S2, MELs synergist formula optimization, S3, herbicide and MELs synergist compounding, S4, standardized pesticide application process step, S5, effect verification and evaluation step and S6, cost optimization and matters needing attention. The MELs synergist adopted in the method has excellent interfacial activity, the 60s contact angle on hydrophobic wheat leaves is smaller than or equal to 36 degrees under the concentration of 2%, the hydrophilic crop leaf surface contact angle is smaller than or equal to 34 degrees, the canvas sedimentation time is as low as 6.75 s and is far better than that of a traditional auxiliary agent Tween 80, through a standardized pesticide application process, the pesticide liquid can be spontaneously and evenly spread on the hydrophilic and hydrophobic leaf surfaces, and the pesticide liquid adhesion rate is remarkably increased. Compared with a traditional method, the leaf surface adhesion rate is increased by 50% or above, and the problem of liquid medicine loss and waste is effectively solved.

The present invention provides a method for producing immobilized cells for producing mannose, and a method for producing mannose, which include the steps of obtaining fermentation liquids of Escherichia coli or Bacillus subtilis in which α-glucan phosphorylase, phosphoglucomutase, glucose phosphate isomerase, mannose 6-phosphate isomerase, and mannose 6-phosphate phosphatase have been expressed by fermentation, and mixing the fermentation liquids to obtain a fermentation mixture.

To provide a method for producing a cyclic diol compound derived from saccharides as a starting material, a cyclic diol compound, and a catalyst for saccharide decomposition.SOLUTION: A method for producing a cyclic diol compound represented by the following general formula (1), comprises a step of reacting glucose or mannose in the presence of zeolite and a metal oxide in an aliphatic alcohol solvent having 1 to 10 carbon atoms, wherein the metal oxide comprises at least one selected from the group consisting of oxides of Group 2 metal elements, oxides of Group 5 metal elements, oxides of Group 6 metal elements, and complex metal oxides, and the complex metal oxide is Mo3Nb2O14 or W3Nb2O14. [In the formula, R represents an aliphatic hydrocarbon group having 1 to 10 carbon atoms].SELECTED DRAWING: None

The invention discloses a refreshing pill and a preparation method thereof, and particularly relates to the technical field of refreshing products. The refreshing pill comprises an outer coating and an inner core, the outer coating comprises 28% of sorbitol, 12% of xylitol, 20% of gum base, 10% of maltitol, 1% of edible flavors and fragrances, 1% of glycerin, 2% of D-mannitol, 5% of Arabic gum, 7% of sucralose, 2% of phospholipid, 2% of palm wax and 2% of shellac, and the inner core is hard edible particles. The inner core is composed of 4% of a refreshing substance and 4% of cane sugar and is used for refreshing people. According to the refreshing pill and the preparation method thereof, through combination of natural components, the refreshing effect is achieved, and meanwhile potential adverse effects on the body are reduced.

The present invention relates to a standardized plant extract of biomass from an in vitro culture of Brassica granatum, containing biologically active compounds and their primary and secondary metabolites, comprising, in% by weight: 4.0 to 6.0 organic acids, 0.5 to 1.5 fatty acids, 8.0 to 12.0 amino acids, 0.5 to 1.0 sterols, 3.0 to 6.0 free phenols, 45 to 55 sugars and 25.0 to 35.0 polyphenols, wherein the content of major mannose glycosides in the polyphenol fraction is 70-96%, making up 18%-35% of the total extract, and to compositions containing the standardized extract and glycerol and to methods for preparing standardized plant extracts. According to the method of the invention, along with the optimally selected steps, specific conditions, parameters such as temperature, duration, agitation, light, growth factors and the like, maximum volume productivity of the target substance and mannoside and stable productivity of in vitro plant cultures are achieved, and the method is a reliable and effective 24 / 7 continuous system for the production of NP.

The invention discloses armillariella mycelium polysaccharide ATMP capable of improving cognitive impairment and a preparation method and application of the armillariella mycelium polysaccharide ATMP. The polysaccharide is composed of two components, and the molecular weights of the two components are 2.30 * 10 < 4 > Da and 4.56 * 10 < 5 > Da respectively; the monosaccharide composition comprises mannose (Man), glucose (Glc) and galactose (Gal), and the molar ratio of the mannose (Man) to the glucose (Glc) to the galactose (Gal) is 1: 16.64: 1.96. Animal experiment results show that the armillariella mycelium polysaccharide ATMP can improve cognitive impairment induced by lipopolysaccharide (LPS) by improving the spatial memory ability of mice, repairing the disorder state and deletion injury of neurons in a hippocampal region and relieving neuroinflammatory response, so that the obvious neuroprotective effect is achieved. As a natural brain-benefiting active component, the armillariella tabescens mycelium polysaccharide ATMP has a wide application prospect in the related fields of brain health.

The invention belongs to the technical field of biological medicines, and particularly discloses a pH and ultrasound double-response type oncolytic microorganism as well as a preparation method and application thereof. On the basis of a tumor targeting platform, a mild thermal response gene loop expression GM-CSF and a surface-coated oncolytic microbial system are integrated, chemotherapeutic drugs are released in a tumor core area, and immunogenic cell death is induced. A thermal response loop is accurately activated through low-intensity focused ultrasound, and engineering bacteria are promoted to express GM-CSF and secrete a large amount of mannose modified OMVs. Due to the nanometer size and mannose targeting of the OMVs, the OMVs are efficiently enriched in lymph nodes, the OMVs are reprogrammed into an immune activation state from an immune tolerance state, and the OMVs and ICD cooperate to promote dendritic cell maturation, tumor antigen presentation and activation of tumor killer T cells, so that a remarkable and powerful treatment effect is achieved in various tumor models, and the application prospect is wide. A new strategy is provided for remodeling the lymph node immune microenvironment and enhancing the anti-tumor immune response.

The application belongs to the technical field of medicines, and discloses a mannose-modified nano silver cluster, a preparation method and application of the mannose-modified nano silver cluster as a probe, wherein the mannose-modified nano silver cluster is obtained by coupling bovine serum albumin-nano silver clusters and modified mannose through acid-amine condensation reaction, 14 modified mannose molecules are coupled on each bovine serum albumin molecule, the mannose-modified nano silver cluster can be applied as a probe in biological recognition and target labeling, a new visual tool is provided for receptor-ligand interaction research and drug delivery monitoring, and the application development of the sugar probe in biomedical detection and diagnosis and treatment is promoted.

Device (1) and system for macrofiltration are disclosed. The macrofiltration comprises mannosylerythritol lipids (MELs) recovery, the MELs being in the form of particles. System comprising the device (1) is also disclosed. The system comprises a first pathway (6) in fluid communication, wherein this first pathway comprises sequentially a first valve (3), a first pump (4), a bioreactor (2) and a second valve (10), and where this first pathway is between the at least one outlet and inlet of the device (1). Process for enhancing mannosylerythritol lipids (MELs) production is also disclosed.

The invention relates to the technical field of pet oral health care, in particular to a compound seaweed source composition for assisting in improving oral health of dogs and cats and application of the compound seaweed source composition. The invention discloses a compound seaweed source composition for assisting in improving oral health of dogs and cats. The compound seaweed source composition comprises sodium alginate and fucoidan, the value of mannuronic acid (M) / guluronic acid (G) in the sodium alginate is 0.96-1.18; the fucoidan is prepared from mannose, glucuronic acid, galactose, xylose and fucose; the weight ratio of the sodium alginate to the fucoidan is 9: 2. The compound seaweed source composition disclosed by the invention has the effects of inhibiting the proliferation of pathogenic bacteria in the oral cavity of a pet, slowing down the rising rate of health indexes of the oral cavity of the pet, inhibiting the generation of volatile organic compounds and ammonia gas in the oral cavity, improving saliva components, slowing down the generation of dental plaque and the like, and can effectively improve the health condition of the oral cavity of the pet.

This invention provides a method for producing recombinant human lysosomal proteins. [Solution] A method for producing recombinant human lysosomal protein is provided, comprising: culturing host cells in a bioreactor that secretes recombinant human lysosomal protein; removing the culture medium from the bioreactor; filtering the culture medium to provide a filtrate; loading the filtrate into an anion exchange chromatography (AEX) column to capture the lysosomal protein; and eluting a first protein product from the AEX column. Such recombinant human lysosomal protein may have a high content of mannose-6-phosphate residues. Pharmaceutical compositions containing such recombinant human lysosomal protein, as well as methods for treating and using such recombinant human lysosomal protein, are also described.

The invention provides yellow-rose yellow-meat boletus fucomannan as well as a composition, a preparation method and application thereof, and belongs to the technical field of biological medicine mass health. The fucomannan has a structure represented by a general formula in an attached drawing of the abstract, and n represents the number of sugar repeating units and is a natural number. The weight-average molecular weight of the fucose glucomannan galactan of the boletus fucose is 10 kDa to 20 kDa, the polydispersity coefficient of the fucose glucomannan galactan is 1 to 2, and the molar ratio of glucose to mannose to fucose to galactose is 1.00 to 3.08 to 2.84 to 14.86. The boletus fucomannan and the composition containing the boletus fucomannan and the composition containing the boletus fucomannan and the composition containing the boletus fucomannan have the effects of promoting proliferation of beneficial bacteria, reducing the pH value of a microenvironment, increasing secretion of SCFAs and lactic acid, adjusting composition of intestinal microorganisms and the like, and have the function of improving the intestinal microecology. Therefore, the invention also discloses the fucomannan-glucomannan of the boletus roseus and the fucomannan-glucomannan of the boletus roseus and the fucomannan-glucomannan of the boletus roseus and the fucomannan-glucomannan of the boletus roseus and the fucomannan of the composition of the boletus roseus and the fucomannan of the composition of the boletus roseus and the fucomannan of the composition of the boletus roseus and the fucomannan of the composition of the composition of the composition of the composition of the composition of the composition of the composition of