870 results about "Mannose" patented technology

The invention discloses a synthesis method of amphiphilic quantum dots and application of the amphiphilic quantum dots in nano oil displacement, and belongs to the field of nano oil displacement. The synthesis method comprises the following steps: heating a mixture containing a carbon source and a nitrogen source in an air atmosphere for reaction, and centrifuging, filtering and drying a reaction product to obtain the amphiphilic quantum dots, the carbon source is selected from at least one of glucose, mannose, citric acid, lignin, chitosan and starch; the nitrogen source is selected from at least one of alkylamine, and the carbon chain length of the alkylamine is C6 to C12. The synthesis method is simple, heating of a high-pressure reaction kettle is not needed, the technological process is simple, and the method is suitable for large-scale industrial production; the series of amphiphilic carbon quantum dots show better solubility and higher absolute fluorescence quantum yield in water and various organic solvents.

The invention provides homogenized pitaya polysaccharide as well as a preparation method and application thereof, and belongs to the technical field of polysaccharide extraction and purification. The homogenized pitaya polysaccharide is a polysaccharide of which the monosaccharide is composed of mannose, glucosamine hydrochloride, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose and arabinose, the relative molecular weight is 8.08 * 10 < 4 > Da, and the corresponding molar ratio is (0.181): (0.250): (16.544): (12.762): (3.793): (0.739): (51.014): (14.716). The invention further provides a preparation method of the homogenized pitaya polysaccharide and application of the homogenized pitaya polysaccharide in preparation of lipid-lowering and sugar-lowering drugs. The homogenized pitaya polysaccharide disclosed by the invention has an obvious lipid-lowering and blood-glucose-reducing effect, is from a natural product, so that the homogenized pitaya polysaccharide has no toxic or side effect and can be used for preparing lipid-lowering and blood-glucose-reducing medicines.

The invention discloses a liver-targeting drug liposome and application thereof, and relates to the field of drug delivery, cholate and mannose are used for respectively modifying phospholipid molecules DSPE-PEG2000, two raw materials DSPE-PEG2000-CA and DSPE-PEG2000-MAN composed of the liposome are synthesized, and then the liposome is prepared into the liver-targeting drug liposome. The liposome with liver targeting property is obtained from the liposome, cholesterol, DSPC, DC-cholesterol and a water-soluble therapeutic drug according to a certain proportion, the liposome has relatively high cell safety and liver targeting property, and the therapeutic effect of the corresponding drug can be remarkably improved after the drug is embedded.

The invention belongs to the field of agricultural chemistry and bio-fertilizer, and discloses a fertilizer synergist based on polyaspartic acid, alginate oligosaccharide and mannan oligosaccharide and a preparation method of the fertilizer synergist. According to the synergist, polyaspartic acid derived from plant source amino acid, alginate oligosaccharide extracted from natural brown algae and mannan oligosaccharide extracted from plants are used as main raw materials, and the polyaspartic acid, the alginate oligosaccharide and the mannan oligosaccharide cooperate to form an induction-complexation-adsorption three-effect-in-one mechanism, wherein the alginate oligosaccharide and the mannan oligosaccharide induce plant root activity and stress resistance gene expression; the polyaspartic acid is dominated to form a multi-point weak complex network, so that slow release and controlled release of nitrogen and potassium ions are realized; the three components cooperate on the surface of soil colloid to form an adsorption layer, and nutrient leaching loss is reduced. The preparation process adopts a green process of'enzymatic pretreatment-segmented complexing-low-temperature forming ', so that the oligosaccharide activity is maintained, and the use of an organic solvent is avoided. The obtained synergist can significantly improve the nitrogen utilization rate and the root activity and prolong the fertilizer efficiency period, and is suitable for green and efficient agricultural production of various crops.

The invention relates to the field of establishment of fingerprints of traditional Chinese medicinal materials, in particular to an establishment method and application of a salvia miltiorrhiza polysaccharide HPLC (High Performance Liquid Chromatography) fingerprint. The establishment method comprises the following steps: preparation of a test solution: taking a salvia miltiorrhiza polysaccharide hydrolysis solution, carrying out derivatization in a reaction bottle, and passing through a 0.1-0.3 [mu] m microporous filter membrane; preparing a reference substance solution, namely weighing mannose, ribose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose and arabinose reference substances, and preparing into a mixed reference substance solution; performing HPLC (high performance liquid chromatography) determination; and establishing the salvia miltiorrhiza polysaccharide HPLC fingerprint spectrum. According to the method, the difference of monosaccharide composition and content in salvia miltiorrhiza medicinal materials from different producing areas and the spectrum-effect relationship between antioxidant activity and anticoagulant activity are clarified, and a certain reference basis is provided for quality control and evaluation of the salvia miltiorrhiza medicinal materials from the aspect of polysaccharide.

The invention relates to the technical field of medicines, and discloses a mannose / trimethyl chitosan modified MXene material (MTC / MXene), a preparation method and application of the mannose / trimethyl chitosan modified MXene material in prevention and treatment of inflammatory bowel diseases. The MTC / MXene material is obtained by preparing mannose-modified trimethyl chitosan and modifying the mannose-modified trimethyl chitosan on a single-layer MXene nanosheet, compared with a pure MXene material, MTC modification endows MXene with hydrophilicity, rich reaction sites are provided for drug loading, higher oxidation resistance is achieved, meanwhile, through surface functional groups and interlayer gaps of the MXene material, the MXene / MTC / MXene material can be used for preparing the drug, and the drug loading efficiency is improved. Different types of drug molecules are loaded and are used for realizing targeted delivery and controlled release of drugs.

The present invention discloses a fermented mucus lactobacillus and its application. The present invention provides a fermented mucus lactobacillus A10 with high extracellular polysaccharide production, and its deposit number is CCTCC NO: M20242082. The fermented mucus lactobacillus A10 has strong bile salt and gastrointestinal digestion tolerance, and has obvious antibacterial effects on Escherichia coli and Staphylococcus aureus. The extracellular polysaccharide synthesized by the fermented mucus lactobacillus A10 is a Wzy type extracellular polysaccharide, which is a heteropolysaccharide composed of rhamnose, galactose, glucose, mannose, and glucuronic acid. It has good thermal stability and antioxidant activity, and can significantly scavenge free radicals. The fermented mucus lactobacillus A10 extracellular polysaccharide provided by the present invention can exert antioxidant activity by activating the Keap1-Nrf2 / ARE signaling pathway, and is expected to be developed into a natural antioxidant that interacts with Keap1-Nrf2 in a targeted manner.

The invention provides essential oil containing ceramide and a preparation method of the essential oil. The essential oil containing ceramide is prepared from ceramide, mannosyl erythritol lipid, synthetic grease and natural vegetable grease. According to the ceramide-containing essential oil disclosed by the invention, complex components such as a surfactant and a stabilizer do not need to be added, and the added mannosyl erythritol lipid is natural sugar ester and has good biocompatibility and safety, so that the ceramide-containing essential oil disclosed by the invention is mild and non-irritant to skin and has excellent product safety; besides, according to the pure oil formulation formula, the purpose of improving the solubility and stability of ceramide can be achieved only by adding mannosyl erythritol lipid, the skin barrier maintaining effect of ceramide can be better played, the anti-inflammatory and skin repairing effects of ceramide are improved, and the skin health level is improved; the application range of ceramide in cosmetics is expanded.

The invention provides a freeze-dried mask. The freeze-dried mask is prepared from the following raw materials in parts by weight: 70 to 90 parts of deionized water, 15 to 25 parts of algae extract, 3 to 5 parts of mannitol, 0.5 to 1 part of glycerol, 0.3 to 0.5 part of hydrolyzed collagen, 1 to 3 parts of amino acid mixture, 4 to 6 parts of hydroxypropyl tetrahydropyrantriol, 3 to 5 parts of tetrahydromethylpyrimidine carboxylic acid and 2 to 4 parts of hyaluronic acid. According to the freeze-dried mask disclosed by the invention, natural anti-wrinkle and moisturizing components such as the hydrolyzed collagen, the algae extract, the amino acid mixture and the hyaluronic acid are locked on the mask cloth in a freeze-drying manner, so that on one hand, the freeze-dried mask can better supplement moisture and nutrition for human skin when being used; on the other hand, the freeze-dried facial mask prepared from the natural beautifying and skin-care components is free of any irritating component during use, and does not cause damage to the human skin.

The method for preparing D-mannose through catalysis can effectively reduce the amount of by-products glucose and fructose, and greatly improves the conversion rate of D-mannose. Specifically, engineering bacteria for expressing isoamylase genes and engineering bacteria for expressing alpha-glucan phosphorylase genes, glucophosphate mutase genes, difunctional enzyme glucophosphate isomerase / mannose 6-phosphate isomerase genes and mannose 6-phosphate phosphatase genes are utilized, starch or starch derivatives are used as substrates, and the starch or starch derivatives are used as substrates. A phosphate buffer solution and Mg < 2 + > are added, a preliminary catalysis system is constructed, after the reaction is completed, an incompletely-reacted substrate and maltodisaccharide exist in the reaction system, the incompletely-reacted substrate and maltodisaccharide can be hydrolyzed into glucose by glucoamylase, and the polyphosphoglucokinase can be used for catalyzing the reaction of the polyphosphoglucokinase under the assistance of sodium hexametaphosphate. All glucose is converted into phosphorylated glucose, then D-mannose is generated, and by-products in a system are removed while the yield is increased.

The present invention discloses an anti-non-small cell lung cancer active birch inonotus polysaccharide IOP-1 and its application, belonging to the field of biomedicine. The present invention adopts a method of boiling water extraction and ethanol precipitation of birch inonotus polysaccharide, and combines DEAE cellulose column purification technology, and utilizes the principle that macromolecular polysaccharides are insoluble in organic solvents to successfully achieve the separation of birch inonotus polysaccharide and obtain a birch inonotus polysaccharide IOP-1 with anti-non-small cell lung cancer activity, wherein the monosaccharide composition of the birch inonotus polysaccharide IOP-1 includes fucose, rhamnose, arabinose, galactose, glucose, xylose and mannose with a molar mass ratio of 2.41:0.58:1.03:2.17:82.20:4.33:7.29. Its significant effect against non-small cell lung cancer was verified by in vitro and in vivo experiments, providing a new treatment method for the clinical treatment of non-small cell lung cancer.

The invention relates to the technical field of natural compounds, in particular to ganoderma leukemia polysaccharide and a preparation method thereof. The ganoderma leukemia polysaccharide comprises galactose, glucose and mannose, and the molar ratio of the galactose to the mannose to the glucose is (1.5-3.5): (15-30): (65-85); the ganoderma leukemia polysaccharide comprises:-> 6)-Glcp-(1-> dominant residues; (-> 6)-Manp-(1->,-> 4)-Glcp-(1-> residue; and-> 4, 6)-Manp-(1->,-> 3, 6)-Galp-(1->. The ganoderma leukemia polysaccharide has anti-oxidation and detoxification capabilities, can avoid neurodegenerative diseases, reduce lipid accumulation and prolong the service life, and also has high-temperature resistance.

The invention discloses a macrophage-targeting inhalable nano material, a preparation method thereof and application of the inhalable nano material in treatment of sepsis myocarditis, and belongs to the technical field of medicines. The nano-material is a nano-liposome, the nano-liposome comprises a mannose modified nano-liposome microsphere, and the mannose modified nano-liposome microsphere comprises a liposome skeleton shell layer, a drug and a targeting layer; the liposome skeleton shell layer comprises soybean lecithin and cholesterol; the medicine comprises quercetin and TPPU (Thermoplastic Polyurethane); and the targeting layer is formed by connecting mannose modified DSPE-PEG2000 to the outer surface of the liposome skeleton shell layer. The nano material can accurately target macrophages at a cardiac inflammation part, has a multi-target-point synergistic effect, is more convenient and faster in administration, remarkably improves the safety and comprehensively improves the treatment effect.

The invention relates to an extraction method and application of a sarcodia montagneana extract for inhibiting bacteria, removing dandruff, controlling oil and relieving, and belongs to the technical field of sarcodia montagneana extracts. The extraction method of the sarcodia montagneana extract comprises the following steps: cleaning fresh sarcodia montagneana with deionized water, soaking the cleaned sarcodia montagneana in a compound solution, grinding the soaked sarcodia montagneana to obtain slurry, freeze-drying the slurry, putting the freeze-dried slurry into a supercritical fluid extractor, adjusting corresponding temperature and pressure, and then extracting the freeze-dried sarcodia montagneana by using a supercritical fluid extractor; and fully contacting the supercritical fluid with the sarcodia montagneana powder, performing extraction treatment, and adjusting pressure through a pressure reducer for separation to obtain the sarcodia montagneana extract. The sarcodia montagneana extract contains galactose, fucose, mannose and glucuronic acid, has a remarkable removal effect on ROS and DPPH free radicals, has high antioxidant activity, can serve as a natural antioxidant and has good effects of inhibiting bacteria, removing dandruff, controlling oil and relieving.

The invention relates to a Kluyveromyces cerevisiae gene modified cell containing GDP-mannose dehydratase polypeptide, which can be applied to synthesis of human milk oligosaccharide, and belongs to the field of synthetic biology. According to the technical scheme, the gene modified cell comprises a recombinant nucleic acid sequence for coding a polypeptide with GDP-mannose dehydratase polypeptide (GDP-mannose dehydratase polypeptide) activity. The polypeptide capable of catalyzing the conversion of GDP-mannose into GDP-4-keto-6-deoxymannose comprises a polypeptide of which the amino acid sequence is at least 70%, 75%, 80%, 85%, 90%, 95%, 99% and 100% identical to the amino acid sequence of SEQ ID NO: 1, SEQ ID NO: 2 and / or the amino acid sequence of polypeptides M1-M13, wherein the amino acid sequence of the polypeptide is at least 70%, 75%, 80%, 85%, 90%, 95%, 99% and 100% identical to the amino acid sequence of the polypeptides M1-M13. The invention provides a kluyveromyces kluyveromyces genetically modified cell with fucosyllactose synthesis activity.

Engineering bacteria for expressing an alpha-glucose phosphate mutase gene, a glucose phosphate mutase gene, a difunctional enzyme glucose phosphate isomerase / mannose 6-phosphate isomerase gene and a mannose 6-phosphate phosphatase gene are used as a whole-cell catalyst, starch or a starch derivative is used as a substrate, phosphate and Mg < 2 + > are added, and the whole-cell engineering bacteria are obtained. A whole-cell catalysis system is constructed, and starch or starch derivatives can be efficiently catalyzed to synthesize D-mannose. According to the method, the D-mannose is prepared through whole-cell catalysis, and the method has the advantages of being low in cost, low in pollution, high in yield and the like and is suitable for industrial production of the D-mannose.

The invention relates to the technical field of medicines, and discloses application of D-mannose in prevention and treatment of androgenetic alopecia. In the prior art, D-mannose can be a potential strategy for treating ferroptosis related diseases, can also be a non-prescription medicine supplement with high safety, and is used for treating neuritis diseases such as multiple sclerosis. The androgenic alopecia mouse model is constructed by utilizing dihydrotestosterone induction, the model mouse is subjected to mannose intervention, and the result shows that application of mannose can promote mouse hair growth, increase cortex thickness, promote hair regeneration and melanogenesis and effectively resist alopecia caused by dihydrotestosterone. The invention provides a new effect of mannose in prevention and treatment of androgenetic alopecia, and also provides a new strategy for prevention and treatment of androgenetic alopecia.

The invention discloses emblic leafflower fruit polysaccharide with sugar-controlling and lipid-lowering activity as well as a preparation method and application of the emblic leafflower fruit polysaccharide. The polysaccharide is prepared by taking fresh phyllanthus emblica pulp as a raw material through hot water extraction, yeast fermentation, desorption after XAD-16 macroporous resin adsorption, alcohol precipitation, ultrafiltration and DEAE-52 cellulose column purification. The low-molecular-weight gulcomannan is separated from phyllanthus emblica for the first time, and monosaccharide composition of the gulcomannan mainly comprises glucose, mannose and galactose and contains trace rhamnose and xylose. The polysaccharide has sugar-controlling and lipid-lowering active action targets in the whole digestive tract (the upper digestive tract: glycolipid digestion and absorption enzymes alpha-amylase, alpha-glucosidase and pancreatic lipase inhibitory activity, starch hydrolysis delay, bile acid adsorption capacity and cholesterol micelle dissolution inhibition capacity; in the lower digestive tract, the multiplication promoting capacity of glycolipid metabolism-related probiotics) shows extremely high biological activity, and can be applied to food and health care products.

The invention discloses ganoderma tsugae polysaccharide with a colorectal cancer relieving effect and a preparation method and application thereof, and belongs to the technical field of biological medicine. The ganoderma tsugae polysaccharide is prepared from mannose, glucose, galactose and fucose, the molar ratio of the mannose to the glucose to the galactose to the fucose is 3.221 to 1.414 to 80.539 to 14.826, and the molecular weight of the ganoderma tsugae polysaccharide is 7.056 kDa. It is proved that the ganoderma tsugae polysaccharide can significantly reduce the number and size of colon tumors; the pathological symptoms such as colon weight index, bleeding, crypt budding, dysplasia and hyperplasia are relieved; compared with the prior art, the ganoderma tsugae polysaccharide provided by the invention has the advantages that the ganoderma tsugae polysaccharide can obviously inhibit the levels of IFN-gamma, TGF-beta and IL-17A in colon and also can obviously inhibit the levels of IFN-gamma, TGF-beta and IL-17A in colon, and meanwhile, the level of IL-4 is improved, so that the ganoderma tsugae polysaccharide provided by the invention has an outstanding action effect in the aspect of relieving colorectal cancer, and a theoretical support is provided for developing colorectal cancer medicines with effectiveness and biological safety.

The invention discloses a pH-responsive deformable dual-targeting nano protein and a preparation method and application thereof, the method comprises the following steps: activating carboxylated mannose through an EDC / NHS system, reacting with an albumin solution, and dialyzing and purifying to obtain mannose modified albumin; and mixing the mannose modified albumin with an inhibitor IRG1-IN-1, and loading the IRG1-IN-1 through a hydrophobic effect to obtain the mannose albumin nanoparticles, ferritin serves as a carrier, oxaliplatin is loaded through a solvent replacement method, and ferritin nanoparticles are obtained; cHO-PEG2k-CHO is used as a coupling agent, and the mannose albumin nanoparticles and the ferritin nanoparticles are crosslinked through a Schiff base reaction, so that the large-size pH-responsive dual-targeting nano-protein is formed. The nano protein has the characteristic of double-targeting separability, so that specific treatment can be realized, immune cell metabolism is interfered while tumor cells are killed, metabolic immunosuppression is blocked, and anti-tumor immunotherapy is promoted.

The invention provides a nano preparation for breaking oxidative stress-inflammation vicious circle and regulating intestinal flora as well as a preparation method and application of the nano preparation. The preparation method of the preparation comprises the following steps: dissolving potassium ferricyanide and polyvinylpyrrolidone in a solution, slowly dropwise adding a zinc acetate dihydrate solution into the solution, stirring, standing, and repeatedly performing ultrafiltration centrifugal washing to obtain zinc-doped Prussian blue; crushing the yeast cells to obtain yeast cell walls; the yeast cell wall and the zinc-doped Prussian blue are repeatedly co-extruded through a liposome extruder to obtain the nano preparation. The ZnPBA in the nano material can efficiently remove active oxygen at an inflammation part and reduce secretion of inflammatory factors, and the oxidative stress-inflammatory vicious circle of ulcerative colitis is broken; meanwhile, the YCW can deliver the ZnPBA to an inflammation part in a targeted manner to kill pathogenic escherichia coli, and mannose components can promote growth of probiotics to realize bidirectional regulation of intestinal flora. The material is simple in preparation method, can be synthesized on a large scale and is used for treating ulcerative colitis.

The invention discloses a production method of fucosylated oligosaccharide. The core of the method is as follows: the GDP-D-rhamnose is converted into the GDP-L-fucose by using the GDP-D-rhamnose-3, 5-epimerase; the GDP-D-rhamnose is obtained by converting the GDP-D-mannose-4, 6-dehydratase and the GDP-4-keto-6-deoxy D-mannose reductase by taking the GDP-D-mannose as a substrate, and the GDP-D-rhamnose is obtained by taking the GDP-D-mannose as a substrate. The invention relates to a method for producing a fucosylated oligosaccharide, which is characterized in that a GDP-D-mannose-4, 6-dehydratase, a GDP-4-keto-6-deoxyD-mannose reductase, a GDP-D-rhamnose-3, 5-epimerase and a fucosyltransferase are expressed in a genetically modified host cell, and the fucosylated oligosaccharide can be produced by the way mentioned above.

The invention relates to the technical field of crop cultivation, and particularly discloses a method for increasing the anthocyanin content of triticale grains, which comprises the following steps: respectively spraying 80-100 times of leaf fertilizer diluent once at the elongation stage and filling stage of triticale growth, and spraying 6-10kg / mu each time; the formula of the foliar fertilizer is as follows: 100 mg to 150 mg of mannose, 100 mg to 150 mg of fructose, 130 mg to 160 mg of mannitol, 160 mg to 200 mg of betaine, 180 mg to 200 mg of alginic acid, 180 mg to 200 mg of fucoidin, 300 mg to 350 mg of amino acid and 1000 g of water. According to the method, the content of anthocyanin in triticale grains can be remarkably increased by spraying 80-100 times of foliar fertilizer diluent prepared by the method in the elongation stage and the pustulation stage of the triticale.

The invention discloses inonotus obliquus polysaccharide IOP-1 capable of resisting non-small cell lung cancer activity and application, and belongs to the field of biological medicine. The method for extracting the inonotus obliquus polysaccharide by boiling water and precipitating the inonotus obliquus polysaccharide by ethanol is combined with a DEAE (diethylaminoethanol) cellulose column purification technology, and the principle that macromolecular polysaccharide is insoluble in an organic solvent is utilized, so that the separation of the inonotus obliquus polysaccharide is successfully realized, and the inonotus obliquus polysaccharide IOP-1 with non-small cell lung cancer resisting activity is obtained. The monosaccharide of the inonotus obliquus polysaccharide IOP-1 comprises fucose, rhamnose, arabinose, galactose, glucose, xylose and mannose, wherein the molar mass ratio of the fucose to the rhamnose to the arabinose to the galactose to the glucose to the xylose to the mannose is 2.41: 0.58: 1.03: 2.17: 82.20: 4.33: 7.29. In-vivo and in-vitro experiments prove that the traditional Chinese medicine composition has a remarkable effect of resisting the non-small cell lung cancer, and a novel treatment method is provided for clinical treatment of the non-small cell lung cancer.

An anti-aging composition for remodeling a dermal structure, a preparation method therefor and a use thereof, relating to the technical field of cosmetics. The anti-aging composition provided comprises the following components in parts by mass: 0.1-5 parts of an alpinia zerumbet leaf extract, 0.01-2 parts of a mixed solution of sodium mannose phosphate and mannose, and 0.01-0.2 parts of retinol substances. The composition provided can promote the generation of collagen and elastin in a reticular layer, enhance the cohesion, enable the reticular layer to have a more compact structure and fiber bundles to be more tightly woven, and promote fibroblast proliferation and metabolism to prevent the extracellular matrix from disintegration, thereby completing remodeling of the dermal structure, toughening the dermal-epidermal junction, and achieving the effect of lifting and firming the skin. The composition can also be prepared into an essence to achieve the effects of ameliorating skin dullness and repairing the skin barrier. The preparation methods for the anti-aging composition and the essence are simple, the raw materials are easily obtained, and the actual production is easy.

The invention belongs to the technical field of preparation of probiotics, and provides a bacterial strain with anti-allergic and immunity-improving effects and application of the bacterial strain. According to the method, a three-layer physical self-assembly structure is adopted, and polysaccharide-polyphenol layer-by-layer self-assembly coating treatment, lipid insertion layer treatment and calcium ion crosslinking protection coating treatment are sequentially carried out. And mannan oligosaccharide MOS and / or galactooligosaccharide GOS are / is mixed in the chitosan layer in a ratio of 0.05-0.15% w / v, and the surface sugar ligand density reaches 4-8 [mu] g per 10 CFU. The coating is constructed under the condition that the pH value is 5.4-5.8; the thickness of the lipid layer is 20-80 nm when the pH value is 5.6-6.2; the cross-linked layer is treated at the pH of 6.0-6.5, so that the gastric acid environment is stable for 2 hours, and the gastric acid is controllably released in intestinal juice for 2-4 hours. The prepared strain has the total thickness of 220-900 nm, the outermost layer coverage rate is larger than or equal to 85%, the gastric juice survival rate is remarkably improved, the strain can be prepared into an oral preparation to be used for preventing and relieving allergic diseases, the viable count of each dose is 1 * 10 <-1 > * 10 <-1 > CFU, and the process is mild and suitable for industrial production.

The invention discloses a composition for proliferating animal bifidobacterium subsp. Lactis and application of the composition, and belongs to the field of probiotics, and the composition comprises a prebiotic composition and a traditional Chinese medicine composition, wherein the mass ratio of the prebiotic composition to the traditional Chinese medicine composition is (5-10): (1-5); the prebiotic composition comprises at least three of mannose oligomer, cotton seed oligosaccharide, isomaltooligosaccharide and galactooligosaccharide; the traditional Chinese medicine composition comprises a Chinese prescription and ginseng powder, and the Chinese prescription comprises at least two of astragalus membranaceus, liquorice and ginger and cinnamon. The composition capable of efficiently proliferating the animal bifidobacterium subsp.lactis is screened out, the proliferation speed and the proliferation amount are both considered, the probiotic effect of probiotics is enhanced, the composition can help proliferation of the animal bifidobacterium subsp.lactis, the advantages of rapidness and high growth are both achieved, and more health effects are achieved.

The invention discloses allium fistulosum and bolete polysaccharide as well as a preparation method and application thereof. The allium fistulosum and bolete polysaccharide is a glycopolymer composed of arabinose, xylose, mannose, glucose and galactose. The allium fistulosum bolete polysaccharide disclosed by the invention has strong antioxidant capacity, has a stimulation and activation effect on macrophage immune response, has an inhibition effect on alpha-glucosidase, has the effects of resisting oxidation, enhancing immunity and reducing blood sugar, and can be applied to development of health-care foods and functional foods.

The invention discloses a preparation method of cordyceps sobolifera polysaccharide and application of the cordyceps sobolifera polysaccharide in relieving acute chemical liver injury. Monosaccharide of the cordyceps sobolifera polysaccharide (abbreviated as CCPW) is composed of glucose, mannose and galactose according to the molar ratio of 8.918: 1: 0.356, and the relative molecular mass is 2.62 * 10 < 4 > Da. Efficacy experiments show that the cordyceps sobolifera homogeneous polysaccharide CCPW can significantly inhibit increase of ALT and AST in a CCl4-induced acute chemical liver injury model mouse, effectively improve the degree of liver cell disruption and swelling, relieve liver inflammatory response, relieve CCl4-induced mouse liver tissue injury, protect mouse intestinal barriers, recover intestinal microbial balance and have obvious liver protection activity. The cordyceps sobolifera polysaccharide CCPW is clear in composition and remarkable in effect, and has wide development and application prospects in the aspect of relieving acute chemical liver injury.

The invention belongs to the field of probiotics and application thereof, and particularly relates to a humanized bifidobacterium animalis probiotic strain and application thereof in preparation of drugs for preventing or treating melanoma. The preservation number of the humanized bifidobacterium animalis is GDMCC No: 65044, the preservation unit is called GDMCC for short, and the active metabolite of the humanized bifidobacterium animalis contains mannose. The composition contains the bifidobacterium animalis probiotic strain, and contains / does not contain metabolites of the bifidobacterium animalis probiotic strain and one or more physiologically acceptable excipients or carriers. The method has the advantages that the protective strain lacked by the melanoma patient is screened and separated from the intestinal tract of a healthy person, the strain can normally survive in the intestinal tract of the human body and has no toxic or side effect, and a new way is provided for prevention and treatment of the melanoma patient by supplementing the lacked protective strain and active metabolites of the patient in a targeted manner; and an innovative solution is provided for auxiliary immunotherapy of melanoma.