4results about How to "High transfection efficiency" patented technology

This invention relates to the field of gene vector technology, and more particularly to an ionizable comb-like polymer, its preparation method, and its applications. This comb-like polymer is a block polymer, comprising at least one block unit. Each block unit includes a first block, a second block, and a third block linked by covalent bonds. It exhibits high molecular flexibility and readily binds to nucleic acid-like substances to form polymer / nucleic acid complexes. Due to the presence of easily degradable intracellular glycosidic bonds in its structure, the polymer demonstrates high transfection efficiency. Furthermore, cell experiments show that the polymer / nucleic acid complex exhibits low toxicity to cells and significantly improves cell survival compared to the positive control. Therefore, it holds promise for widespread application in cell transfection. The preparation method provided by this invention utilizes a redox amination tandem reaction to directly prepare the target product, eliminating the need for intermediate product purification processes. This simple process facilitates large-scale production.

The present application relates to the technical fields of biological medicine and gene therapy, and particularly relates to a protonatable cationic lipid, a nucleic acid delivery system and application thereof. The protonatable cationic lipid has a structure as shown in formula (I), and contains a protonatable group such as a hydroxyl group or an amino group at the end. The protonatable cationic lipid can form a lipid nanoparticle for loading various nucleic acid substances. The lipid nanoparticle nucleic acid delivery system has high cell transfection efficiency in vitro, and can be targeted to organs such as liver in vivo to achieve efficient gene editing. The lipid nanoparticle constructed by the protonatable cationic lipid provided by the present application has high encapsulation efficiency, good stability and excellent delivery efficiency, and has a wide application prospect in the fields of gene therapy and cell engineering.

The application provides a structural lipid compound and a preparation method and application thereof. The structural lipid compound has a structure shown in formula I. The structural lipid compound provided by the application can replace traditional neutral phospholipids to prepare drug-loaded lipid nanoparticles. The drug-loaded lipid nanoparticles containing the structural lipid compound have small particle sizes, uniform particle size distribution, and good loading and delivery effects on nucleic acid drugs.

The application discloses a construction method and application of a SOX18 lentivirus recombinant tag carrier stably overexpressed in sheep hair papilla cells, and the method comprises the following steps: amplifying a full-length CDS product of a sheep SOX18 gene, double-enzyme cutting a carrier, connecting the full-length CDS amplification product of the SOX18 gene with a linearized carrier, and expanding the recombinant carrier, mixing the SOX18 lentivirus recombinant tag carrier with a lentivirus packaging auxiliary plasmid and an auxiliary plasmid, and transfecting 293T cells to realize packaging of the SOX18 lentivirus recombinant tag carrier, and transfecting the SOX18 lentivirus recombinant tag carrier into sheep hair papilla cells, and it is found that overexpression of SOX18 can inhibit apoptosis of the sheep hair papilla cells. The application significantly improves the transfection efficiency of the hair papilla cells, greatly increases the probability of integration of the SOX18 gene into the genome of the hair papilla cells, constructs a high-level in-vitro expression model of SOX18, and proposes an application of the SOX18 gene in inhibiting apoptosis of the hair papilla cells.