41 results about "Start site" patented technology

The present invention relates to methods and devices for identifying and quantifying, including low abundance, nucleotide base mutations, insertions, deletions, translocations, splice variants, miRNA variants, alternative transcripts, alternative start sites, alternative coding sequences, alternative non-coding sequences, alternative splicings, exon insertions, exon deletions, intron insertions, or other rearrangement at the genome level and/or methylated nucleotide bases.

The present invention relates to a PCR primer facilitating hot-start PCR by suppressing non-specific amplification at room temperature and at the same time capable of reducing significantly non-specific amplification by dominating the amplification of the PCR product rather than the amplification of the original template from the third PCR cycle, more precisely a PCR primer prepared by additionally inserting the reverse-complementary sequence to a certain region starting from the 5′-start site of the 5′-terminus of the original primer which is composed of priming sequence to anneal to a PCR template into the 5′-terminus of the original primer and a PCR method using the same. The primer of the present invention has a original primer sequence composed of priming sequence to anneal to a PCR template and an additional reverse-complementary sequence, which inserted into the 5′-terminus of the original primer, to a certain region starting from the 5′-start site of the 5′-terminus of the original primer sequence, suggesting that a template-specific sequence and its reverse-complementary sequence are included in the same primer. The present invention can improve PCR specificity by reducing non-specific amplification.

The invention relates to a screening method of silkworm hemocyte specific expressed genes and a method for analyzing and finding a hemocyte specific expression regulation element through cloning of a protease O gene promoter. The hemocyte specific expression regulation element is manufactured through the following steps that 1, a hemocyte specific expression candidate gene protease O gene is screened out through silkworm tissue chip expression data, transcriptome data, a quantitive RT-PCR and other methods, and the silkworm hemocyte specific expression of the gene is verified successfully; 2, a related gene promoter is successfully cloned, and functional analysis is carried out; 3, a Bac-to-Bac rhabdovirus expression system is successfully improved, an effective hemocyte promoter detection system is built, the activity of the candidate gene promoter is detected at the cell and the individual level, and tissue specificity of the promoter is verified; 4, the silkworm hemocyte specific gene protease O gene promoter is obtained through the study, and it is analyzed and found that the promoter is provided with the tissue specificity regulation element in the section between the upstream -333 and -80 of a transcriptional start site. The specificity expression of the gene on hemocyte is controlled.