201results about "Protozoa" patented technology

The invention belongs to the field of gene engineering, and relates to a gene-deleted recombinant toxoplasma gondii strain vaccine and a construction method thereof, in particular to a gene-deleted recombinant toxoplasma gondii strain, a drug and / or a biological product containing the gene-deleted recombinant toxoplasma gondii strain, a construction method and application of the gene-deleted recombinant toxoplasma gondii strain. The toxoplasmosis vaccine with attenuated activity developed based on the recombinant toxoplasmosis strain is suitable for immunoprophylaxis of toxoplasmosis of various mammals, can block transmission of toxoplasmosis to people and other animals, and is especially suitable for immunoprophylaxis of cat toxoplasmosis infection and / or toxoplasmosis. The vaccine is easy to produce, high in safety, excellent in efficacy, long in immunization duration, capable of distinguishing vaccine immunization from natural infection, free of pollution to animals and the environment, capable of effectively reducing residues of toxoplasma oocysts in the environment and reducing the risk that people are infected with toxoplasmosis, and has huge application potential and important public health significance.

The invention discloses a toxoplasma gondii attenuated vaccine strain RH delta rop67 as well as a construction method and application thereof, and belongs to the technical field of parasitic disease prevention and control and biological product preparation. The attenuated vaccine strain is constructed by performing targeted knockout on the ROP67 gene in a toxoplasma gondii strain RH delta ku80 through a CRISPR / Cas9 mediated gene editing technology. Compared with a wild type strain, the attenuated vaccine strain shows remarkable attenuation characteristic and good immunogenicity. A test result shows that the attenuated vaccine strain can induce a host to generate specific immune response mainly based on cellular immunity, maintains a protection effect on toxoplasma gondii infection in a relatively long immune period, and has a protection effect on tachyzoite infection and a chronic infection stage of toxoplasma gondii strains with different virulence; the survival ability of a host to tachyzoite infection can be improved, and the formation level of cysts in tissues is reduced. The invention provides a technical scheme with long-term immune potential for research and development of toxoplasma gondii attenuated vaccines.

The invention discloses a toxoplasma gondii attenuated strain as well as a construction method and application thereof. The toxoplasma gondii attenuated strain delta MIC1 delta MIC3 disclosed by the invention is obtained by knocking out MIC1 and MIC3 genes from a TIR1 strain by utilizing a CRISPR-Cas9 (clustered regularly interspaced short palindromic repeats-associated 9) technology. The toxoplasma gondii attenuated strain delta MIC1 delta MIC3 can effectively enhance macrophage functions including phagocytic ability, proinflammatory factor secretion and the like, and provides theoretical basis and technical support for clarification of an immunoregulation network of toxoplasma gondii-host interaction and development of vaccine design based on attenuated strains.

The invention belongs to the technical field of plant growth-promoting bacteria, and particularly relates to protozoa trichomonad cooperating with bacillus to prevent and control cucumber fusarium wilt and application of the protozoa trichomonad. The invention provides a strain of protozoa trichomonas mtoleri (Cercomonas mtoleri) B5, and the preservation number of the strain is CCTCC (China Center for Type Culture Collection) N0: V202502. The protozoa trichomonad B5 provided by the invention can induce spore germination of a biocontrol strain bacillus velezensis SQR9 and improve the rhizosphere colonization capability of the biocontrol strain SQR9. The protozoa trichomonad B5 and the bacillus velezensis SQR9 are jointly inoculated in cucumber fusarium wilt continuous cropping soil, and the fusarium wilt morbidity of cucumber plants can be effectively reduced.

The invention discloses an antigen fusion protein and application thereof in preparation of a live vaccine for preventing infectious bursal disease. Specifically disclosed are antigen fusion proteins comprising a VP2 protein, a trimer tag, and a C3d protein. The invention also discloses recombinant Eimeria heap constructed by using the antigen fusion protein and application of the recombinant Eimeria heap in preparation of IBDV live vaccines. The oocyst of the recombinant Eimeria heap can effectively induce humoral immune response aiming at IBDV as a vaccine active ingredient, the level of an induced antibody is obviously higher than that of an insect strain expressed by a single VP2 antigen, viruses can be more effectively neutralized, the immune protection efficacy is improved, the safety is high, and the oocyst has the potential of serving as a live vector genetic engineering vaccine. The IBDV live vaccine can be orally taken through drinking water or feed, vaccination is simple, large-scale immunization of chicken flocks can be achieved, and the IBDV live vaccine is economical and efficient and has a wide prospect of being applied to prevention and control of the IBDV of the chicken flocks.

The invention belongs to the technical field of biology, and particularly relates to a simple reniform worm enlarged culture and reniform worm cyst resuscitation method, which is characterized in that a wheat seedling culture solution is mixed with a reniform worm culture solution of escherichia coli, and the method comprises the following steps: carrying out small-range high-density culture on reniform worm; forming a reniform worm cyst and preserving breeds; resuscitating the reniform worm cyst; the resuscitated kidney-shaped worm can also be subjected to enlarged culture. The method is suitable for culture and cyst resuscitation of various kidney-shaped worms, the culture efficiency of the kidney-shaped worms is greatly optimized, the time required for the kidney-shaped worms to grow to the highest density is shortened, and resuscitation of dormant cysts of the kidney-shaped worms and the recovery speed of population density after resuscitation are both remarkably increased. The method can be applied to researches on morphological classification, cytobiology, ecology and the like of the reniform worm, research on environmental monitoring and treatment of the reniform worm in soil heavy metal pollution and the like, and research on preparation of microbial fertilizers from the reniform worm and the like.

Provided are Streptomyces and a use thereof. The Streptomyces is named Streptomyces linyiensis, which was preserved in China General Microbiological Culture Collection Center (CGMCC) on 28 September 2022, and has a preservation number of CGMCC No. 25834. A Streptomyces fermentation broth can be used to significantly improve the abundance of nitrogen-fixing microorganisms in crop rhizosphere soil, improve the nitrogen-fixing enzyme activity of the crop rhizosphere soil, improve the nitrogen-fixing capability of the nitrogen-fixing microorganisms in the crop rhizosphere soil, and promote the absorption of nitrogen nutrients by crops, and can be used for preparing bacterial agents, microbial fertilizers or biopesticides having corresponding functions.

The invention relates to protozoa reniform worm and application thereof in promoting saline-alkaline tolerant growth of corn, protozoa swollen reniform worm ISS-TY1 has been preserved in a preservation unit specified by the State Intellectual Property Office on April 22, 2025, the preservation unit name is China Center for Type Culture Collection, and the preservation number is CCTCC NO: C202521. Research finds that the biomass of the upper part of the corn field treated by applying the reniform enlargement worm ISS-TY1 is increased by 183%-197%, the abscisic acid content of the root is remarkably reduced by 30.3%-43.1%, the K < + > content of the leaf is increased by 20.7%-29.2%, and the Na < + > content is reduced by 18.5%-26.8%. The invention reveals that the reniform swollen worm ISS-TY1 has huge application potential and prospect in the aspects of improving the saline-alkaline resistance of crops and promoting the growth of the crops.

The invention belongs to the field of biology, and discloses a cdc42 gene knockout HCT-8 cell line and application thereof, the taxonomic name of the cdc42 gene knockout HCT-8 cell line is Homo sapiens, the cdc42 gene knockout HCT-8 cell line is preserved in Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC NO: 66616; the preservation date is July 01, 2025; the preservation address is the fifth floor of building 59, No.100 courtyard, Xianlie Middle Road, Guangzhou City, Guangdong Province. According to the invention, a cdc42 gene in an HCT-8 cell is knocked out by adopting a CRISPR-Cas9 technology so as to construct a cdc42 gene knocked-out HCT-8 cell line, and the cdc42 gene knocked-out HCT-8 cell line is used as a cryptosporidium parvum cell infection model.

The present invention relates to a method for decomposing peracetic acid using a metal compound and a method for culturing a microorganism using the same, and more particularly, to a method for decomposing peracetic acid using a metal compound and a method for culturing a microorganism using the same, whereby a microorganism can be cultured in a sterilized culture medium by significantly rapidly decomposing peracetic acid when using the method of the present invention.

The invention relates to in vivo generation of beneficial molecules using genetically modified, nonpathogenic microorganisms. The invention has application to various fields, such as medicine, human performance, and veterinary medicine. In various embodiments, the use of transgenic Trypanosoma rangeli, a nonpathogenic microorganism may be purposefully engineered, altered, and designed to be a blood symbiont in humans and animals after genetic modification to express foreign nucleic acid sequences that encode proteins, polynucleotides, and / or pathways to treat disease or enhance specific aspects of host physiology to include metabolic dominance to correct or augment homeostatic imbalances when suitable. The foreign nucleic acid sequences may be used to generate enzymes, scavenger molecules, peptides and hormones, antibodies, nanobodies, signaling ligands, and other therapeutic or novel synthetic agents.

The purpose of the present disclosure is to provide a nicotinamide adenine dinucleotide (NAD) production promoter derived from a novel material, and a method for producing the NAD production promoter. The technology disclosed herein relates to an NAD production promoter and is characterized by containing a Euglena-derived substance, which is a component derived from microalgae belonging to the genus Euglena. It is confirmed that the NAD production promoter has an NAD-production-promoting action on a living animal body. Because NAD production promoter uses microalgae belonging to Euglena as a material, the NAD production promoter can be stably supplied by means of culturing.

The invention discloses a construction method and application of a toxoplasma gondii polygene deletion vaccine strain. According to the invention, a TgBFD1 gene and a TgDAHPS gene are respectively knocked out from an ME49 [delta] 6pgdh1 insect strain through a gene editing technology, and a polygene deleted insect strain [delta] 6pgdh1 [delta] bfd1 [delta] dahps is successfully constructed; the strain can be subcultured in vitro, has the characteristics of no toxicity and no formation of tissue cysts in a host body, has better immune protection after immunization and inoculation, and can resist lethal infection of highly toxic strains. The toxoplasma gondii polygene deletion strain provided by the invention has better safety than ME49 delta 6pgdh1 strain, the toxicity of the toxoplasma gondii polygene deletion strain is obviously reduced, tissue cysts are not formed, the toxoplasma gondii polygene deletion strain can effectively resist infection of wild poisonous strains, the toxoplasma gondii polygene deletion strain has good immune protection capability, the defects that ME49 delta 6pgdh1 still has toxicity and can form tissue cysts are overcome, and the toxoplasma gondii polygene deletion strain has good application prospects. A method is provided for developing a safe and efficient toxoplasma gondii vaccine.

The invention discloses a cryptosporidium parvum AOX gene deleted strain as well as a construction method and application thereof, and the cryptosporidium parvum AOX gene deleted strain disclosed by the invention is obtained by editing an AOX gene of cryptosporidium parvum through a gene editing technology. According to the gene editing technology, the cryptosporidium parvum AOX gene is knocked out by using a CRISPR / Cas9 gene editing technology, so that a cryptosporidium parvum AOX gene deleted strain is obtained; the AOX gene is found to be related to insect toxicity for the first time, and the infection intensity of an AOX gene deleted insect strain in a gamma interferon gene knockout mouse body is remarkably reduced. Research shows that after the AOX gene is deleted from the wild type cryptosporidium parvum IIdA20G1 subtype strain, the strain has the advantage that the toxicity is obviously weakened, and can be used for preparing cryptosporidium parvum attenuated vaccines so as to prevent cryptosporidium parvum infection.

The invention discloses a toxoplasma gondii attenuated strain with deletion of a Tgsrs51 gene and application of the toxoplasma gondii attenuated strain, and belongs to the technical field of gene engineering. A Tgsrs51 gene is deleted in a genome of the toxoplasma gondii attenuated strain; and the ID (Identity) number of the Tgsrs51 gene on a ToxoDB (Database) website is TGME49308840. A Tgsrs51 gene deleted strain of a toxoplasma gondii type II Pru strain is constructed by utilizing a CRISPR-Cas9 technology, and experiments prove that the Tgsrs51 gene deletion can remarkably reduce the invasion efficiency of the toxoplasma gondii Pru strain, the secretion capacity of micronematosome MIC2 and the tachyzoite proliferation rate, lead to in-vitro growth limitation, remarkably improve the survival rate of infected mice and remarkably reduce the number of intracerebral cysts. The invention provides a theoretical basis for researching a molecular mechanism and an immune mechanism of toxoplasma gondii pathopoiesis, and provides a new strategy for prevention and control of toxoplasmosis.

A filter assembly includes a casing having of a polymeric film and bounding a compartment, the casing having an inlet opening communicating with the compartment and a first outlet opening communicating with the compartment. A first filter includes a porous filter body through which gas can pass and has a pore size smaller than 1 µm, the first filter being coupled with the casing so that the filter body is at least partially disposed within compartment of the casing and so that gas passing through the compartment of the casing from the inlet opening to the first outlet opening must pass through the filter body.

The present invention relates to a method for decomposing peracetic acid using a metal compound and a method for culturing microorganisms using the decomposition method. The cultivation of microorganisms using the method of the present invention allows an effective removal of the peracetic acid used in a culture medium for sterilization.

The invention provides a toxoplasma gondii gene deletion strain with an immune protection effect and application of the toxoplasma gondii gene deletion strain, and belongs to the technical field of veterinary parasitology, veterinary immunology and parasite molecular biology. According to the toxoplasma gondii gene deletion strain, a gamma-glutamyl hydrolase gene is deleted. The invention also provides a method for constructing the insect strain. The method comprises the following steps: constructing a circular plasmid pSAG1-Cas9-U6-sgGGH; constructing a repair template containing upstream and downstream homologous arms of the gamma-glutamyl hydrolase gene and a DHFR resistance gene; co-transfecting the annular plasmid and the repair template to a toxoplasma gondii ME49 strain; performing drug screening on pyrimethamine to obtain positive monoclone; and confirming that the GGH gene is completely knocked out through PCR (Polymerase Chain Reaction) detection, sequencing analysis and immunofluorescence detection. The toxoplasma gondii gene deletion strain can provide a remarkable protection effect on toxoplasma gondii infection and is high in safety.

The invention provides an in-vitro induction and purification method for Giardia lamblia cysts. The induction method is characterized in that Giardia lamblia trophozoite is cultured in a TYI-S-33 culture medium containing pulvis fellis suis to obtain the Giardia lamblia cysts. According to the in-vitro induction method for the Giardia lancea cysts, the pig gall powder is added into the TYI-S-33 culture medium to induce the Giardia lancea trophozoite, and it is found that compared with common ox gall powder, the proportion of converting the Giardia lancea trophozoite into the cysts can be remarkably increased, and the generation rate of the induced cysts stably reaches 50%; according to the giardia lamblia cyst purification method disclosed by the invention, protease K is utilized for purification, so that pure cysts can be obtained, long-term stability and activity can be maintained, and the cysts can be stably stored for several weeks to several months at 2-8 DEG C and can be prepared into giardia lamblia cyst standard substances; the method is used for method verification, quality control, disinfection efficiency research, filtration efficiency evaluation and the like.

The invention belongs to the technical field of marine environmental protection, and discloses a biological treatment method for efficiently removing red tide algae by utilizing ciliates, which comprises the following steps: culturing and treating ciliates; removing red tide algae; and data processing and analysis and red tide treatment. The method is high in removal efficiency, and experiments show that in a red-tide algae culture solution with the initial density of 3000cells / mL, the infusorians can reduce the abundance of the red-tide algae to be close to 0 (the removal rate reaches 99%) within 7 days. The method shows a remarkable removal effect on red tide algae with different initial concentrations, and is suitable for large-scale red tide treatment. The use of the ciliates is consistent with the natural ecological chain of the red tide algae, so that the problem of environmental pollution caused by a traditional treatment method is avoided.

The invention discloses a method for breeding, separating and purifying nosema bombycis, which comprises the following steps: infecting 2-year-old silkworms for 6-12 hours by using nosema bombycis with the concentration of 1.0 * 10 < 5 > spores / mL to 5.0 * 10 < 5 > spores / mL, feeding the silkworms with clean mulberry leaves until the silkworms fall asleep at 4 years old, and gradually causing pebrine death when the silkworms grow at 5 years old; the method comprises the following steps: grinding dead silkworms to prepare grinding liquid, filtering the grinding liquid, and separating out filtrate enriched with nosema bombycis; centrifuging the filtrate to obtain a precipitate which comprises grey silkworm body tissues on the upper layer and white nosema bombycis on the lower layer, shaking the centrifuge tube by using a shaking table to release the grey silkworm body tissues to supernate, pouring out the supernate, and retaining the white nosema bombycis at the bottom of the centrifuge tube. According to the method, the nosema bombycis is bred, separated and purified according to the growth rules and characteristics of the bombyx mori and the nosema bombycis, a high-quality sample can be efficiently obtained, and the nosema bombycis is high in content and few in impurity.

The present invention provides a protozoan Trichomonas cercoides for use in improving wheat yield and soil organic matter content. Protozoan Trichomonas cercoides NJAU‑Z1 (Cercomonas direct) NJAU‑Z1), with a preservation number of CCTCC NO: C202443. Field trials have found that the average yield of wheat treated with protozoan Trichomonas cercoides NJAU‑Z1 increased by 11.23%. At the same time, the organic matter content of the soil treated with protozoan Trichomonas cercoides NJAU‑Z1 increased by 9.2%. The present invention shows that protozoan Trichomonas cercoides Cercomonas direct The application of NJAU‑Z1 has great potential in increasing soil organic matter content, soil fertility and grain yield.

The invention belongs to the technical field of biology, and provides an application of alpha-amylase protein derived from nephroplasmosis in culture of nephroplasmosis, and the alpha-amylase protein is obtained by synthesizing a gene sequence of alpha-amylase as shown in SEQ ID NO.2 after codon optimization and then transfecting the gene sequence to an expression vector. And carrying out bacterial transformation, culture, induction and crushing. The invention also provides a method for inducing the formation of the reproductive cyst of the reniform worm. It is found for the first time that alpha-amylase protein specifically induces reproductive cysts to be formed, the biomass of the nephroplasmosis is increased, the growth generation of the nephroplasmosis is shortened, and the method is suitable for species such as distended nephroplasmosis and nephroplasmosis henslouianum and fits natural ecological scenes; complex equipment is not needed, the cost is low, large-scale production is easy, the stability is high, and the method can be widely applied to soil ecological restoration, agricultural microbial resource development and basic research related to nephroplasmosis.

Provided is a means for constructing a modified genomic DNA which has a nucleotide sequence different from an existing genomic DNA sequence, contains a modified gene capable of expressing a polypeptide, a functional RNA, or both, and does not contain exogenous DNA.　This method comprises: performing, at least twice, (a) a step for specifically cleaving genomic DNA of a cell into at least two target sequences by means of a sequence-specific nuclease, and linking the resultants through a DNA double-stranded cleavage repair by the cell; and deleting at least two regions from an existing gene and non-coding regions adjacent thereto to thereby produce a modified gene.

The application discloses a Toxoplasma gondii DAHPS gene deletion vaccine strain and application thereof. The application provides a Toxoplasma gondii gene knockout strain, which is obtained by directly knocking out a 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase TgDAHPS gene of the Toxoplasma gondii through a gene editing technology, the gene ID of the TgDAHPS gene is TGGT1_221260, and the nucleotide sequence is shown as SEQ ID NO. 1. The gene deletion strain Δdahps provided by the application can grow normally in vitro, does not reproduce in a host in vivo, and is almost non-toxic. After immunization of the Δdahps strain, the host can be induced to have good immune protection. The Δdahps has the advantages of weak virulence and almost no reproduction in vivo as the attenuated vaccine strain of the Toxoplasma gondii, can improve the resistance of the host to wild Toxoplasma gondii, and can be used for preventing the infection of the Toxoplasma gondii in humans and animals, and has important significance for developing more Toxoplasma gondii vaccines.

[Problem] It is an object of the present invention to provide a method for collecting seawater that contains plankton and producing DHMBA, which is an antioxidant, from the plankton contained in the seawater.[Solution] The method of the present invention includes: filtering collected seawater containing the plankton using a filter; taking out a cell content from the plankton remaining on the filter; subsequently heating / pressurizing the cell content taken out; and producing 3,5-dihydroxy-4-methoxybenzyl alcohol from the heated / pressurized product. The plankton is a diatom.

The invention discloses a locust microsporidia strain and a preparation and use method thereof, and relates to the technical field of locusts, the locust microsporidia strain and locust microsporidia have the preservation number of CGMCC No.19390 and are named as PL-GM1 strain. The preparation method of the locust microsporidia strain comprises the following steps: S1, screening high-temperature-resistant mutant strains; s2, other high-temperature-resistant microorganisms are used in a combined mode; s3, cultivating healthy insects; s4, healthy insect inoculation; s5, feeding diseases and pests; s6, microspores are processed and inspected, packaging and quality inspection are conducted, and finally freezing storage is conducted. According to the method, high-temperature-resistant mutant strains are screened, high-temperature-resistant culture can be conducted on the locust microsporidia of the PL-GM1 strain, and the locust microsporidia of the PL-GM1 strain can have the high-temperature-resistant characteristic; other high-temperature-resistant microorganisms are used in a combined mode, the microsporidia screened in the step S1 and the other high-temperature-resistant microorganisms are co-cultured, and the high-temperature-resistant capacity and the prevention and treatment effect of the microsporidia are enhanced.

The invention discloses a toxoplasma gondii double-gene deletion attenuated vaccine strain as well as a construction method and application thereof. The toxoplasma gondii double-gene deletion attenuated vaccine strain is constructed by deleting a ku80 gene and a CDC14 gene on the basis of a toxoplasma gondii type II PLK strain, and is named as PLK [delta] TgKu80 [delta] TgCDC14 ([delta] CDC14 for short), and experimental results show that the pathogenicity of the strain is remarkably reduced after the CDC14 gene is knocked out. In order to further explore the immune protection effect on toxoplasma gondii acute infection mice, secondary acute infection is carried out on mice immunized with delta CDC14 strains, and the result shows that compared with non-immunized group mice, no immunized mice die within 30 days, and all non-immunized mice die within 13 days. Therefore, the double-gene deletion attenuated vaccine strain has the potential of being used as a vaccine, and an effective technical means is provided for preventing toxoplasma gondii infection.

The present disclosure is directed to malaria-causing parasites, specifically Plasmodium species parasites, and more specifically Plasmodium species parasites that have been genetically modified to develop normally only to the late liver stage, but are completely inhibited from entering the blood stage or infection of red blood cells. Specifically, the inventors have identified a genetic modification resulting from disruption of the genetic function of the liver stage nuclear protein (LINUP) gene that results in a late liver stage arrest of the development of these parasites. This complete late liver stage arrest allows enhanced expression of the parasite antigen array during liver stage development, but prevents entry into the blood stage, red blood cell infection, and associated signs, symptoms, and pathology of malarial disease.

The invention belongs to the technical field of biological medicine, and discloses a toxoplasma gondii in-vitro merozoite induction model and a construction method thereof. The invention discloses application of a glutamine antagonist to induction of differentiation of toxoplasma gondii tachyzoite to merozoite or preparation of a product for induction of differentiation of toxoplasma gondii tachyzoite to merozoite, and finds that the glutamine antagonist JHU083 can induce differentiation of toxoplasma gondii virulent strain RH and attenuated strain ME49 tachyzoite into merozoite under in-vitro conditions for the first time. A reliable in-vitro model is provided for researching the key initial stage of sexual reproduction of the toxoplasma gondii, and an irreplaceable research foundation is laid for the key link of targeted blocking of parasite transmission.