74results about "Protozoa" patented technology

The invention discloses a toxoplasma gondii attenuated vaccine strain RH delta rop67 as well as a construction method and application thereof, and belongs to the technical field of parasitic disease prevention and control and biological product preparation. The attenuated vaccine strain is constructed by performing targeted knockout on the ROP67 gene in a toxoplasma gondii strain RH delta ku80 through a CRISPR / Cas9 mediated gene editing technology. Compared with a wild type strain, the attenuated vaccine strain shows remarkable attenuation characteristic and good immunogenicity. A test result shows that the attenuated vaccine strain can induce a host to generate specific immune response mainly based on cellular immunity, maintains a protection effect on toxoplasma gondii infection in a relatively long immune period, and has a protection effect on tachyzoite infection and a chronic infection stage of toxoplasma gondii strains with different virulence; the survival ability of a host to tachyzoite infection can be improved, and the formation level of cysts in tissues is reduced. The invention provides a technical scheme with long-term immune potential for research and development of toxoplasma gondii attenuated vaccines.

The present invention relates to a method for decomposing peracetic acid using a metal compound and a method for culturing microorganisms using the decomposition method. The cultivation of microorganisms using the method of the present invention allows an effective removal of the peracetic acid used in a culture medium for sterilization.

The invention provides a toxoplasma gondii gene deletion strain with an immune protection effect and application of the toxoplasma gondii gene deletion strain, and belongs to the technical field of veterinary parasitology, veterinary immunology and parasite molecular biology. According to the toxoplasma gondii gene deletion strain, a gamma-glutamyl hydrolase gene is deleted. The invention also provides a method for constructing the insect strain. The method comprises the following steps: constructing a circular plasmid pSAG1-Cas9-U6-sgGGH; constructing a repair template containing upstream and downstream homologous arms of the gamma-glutamyl hydrolase gene and a DHFR resistance gene; co-transfecting the annular plasmid and the repair template to a toxoplasma gondii ME49 strain; performing drug screening on pyrimethamine to obtain positive monoclone; and confirming that the GGH gene is completely knocked out through PCR (Polymerase Chain Reaction) detection, sequencing analysis and immunofluorescence detection. The toxoplasma gondii gene deletion strain can provide a remarkable protection effect on toxoplasma gondii infection and is high in safety.

The invention provides an in-vitro induction and purification method for Giardia lamblia cysts. The induction method is characterized in that Giardia lamblia trophozoite is cultured in a TYI-S-33 culture medium containing pulvis fellis suis to obtain the Giardia lamblia cysts. According to the in-vitro induction method for the Giardia lancea cysts, the pig gall powder is added into the TYI-S-33 culture medium to induce the Giardia lancea trophozoite, and it is found that compared with common ox gall powder, the proportion of converting the Giardia lancea trophozoite into the cysts can be remarkably increased, and the generation rate of the induced cysts stably reaches 50%; according to the giardia lamblia cyst purification method disclosed by the invention, protease K is utilized for purification, so that pure cysts can be obtained, long-term stability and activity can be maintained, and the cysts can be stably stored for several weeks to several months at 2-8 DEG C and can be prepared into giardia lamblia cyst standard substances; the method is used for method verification, quality control, disinfection efficiency research, filtration efficiency evaluation and the like.

The invention discloses a method for breeding, separating and purifying nosema bombycis, which comprises the following steps: infecting 2-year-old silkworms for 6-12 hours by using nosema bombycis with the concentration of 1.0 * 10 < 5 > spores / mL to 5.0 * 10 < 5 > spores / mL, feeding the silkworms with clean mulberry leaves until the silkworms fall asleep at 4 years old, and gradually causing pebrine death when the silkworms grow at 5 years old; the method comprises the following steps: grinding dead silkworms to prepare grinding liquid, filtering the grinding liquid, and separating out filtrate enriched with nosema bombycis; centrifuging the filtrate to obtain a precipitate which comprises grey silkworm body tissues on the upper layer and white nosema bombycis on the lower layer, shaking the centrifuge tube by using a shaking table to release the grey silkworm body tissues to supernate, pouring out the supernate, and retaining the white nosema bombycis at the bottom of the centrifuge tube. According to the method, the nosema bombycis is bred, separated and purified according to the growth rules and characteristics of the bombyx mori and the nosema bombycis, a high-quality sample can be efficiently obtained, and the nosema bombycis is high in content and few in impurity.

The invention belongs to the technical field of biology, and provides an application of alpha-amylase protein derived from nephroplasmosis in culture of nephroplasmosis, and the alpha-amylase protein is obtained by synthesizing a gene sequence of alpha-amylase as shown in SEQ ID NO.2 after codon optimization and then transfecting the gene sequence to an expression vector. And carrying out bacterial transformation, culture, induction and crushing. The invention also provides a method for inducing the formation of the reproductive cyst of the reniform worm. It is found for the first time that alpha-amylase protein specifically induces reproductive cysts to be formed, the biomass of the nephroplasmosis is increased, the growth generation of the nephroplasmosis is shortened, and the method is suitable for species such as distended nephroplasmosis and nephroplasmosis henslouianum and fits natural ecological scenes; complex equipment is not needed, the cost is low, large-scale production is easy, the stability is high, and the method can be widely applied to soil ecological restoration, agricultural microbial resource development and basic research related to nephroplasmosis.

The invention discloses a toxoplasma gondii double-gene deletion attenuated vaccine strain as well as a construction method and application thereof. The toxoplasma gondii double-gene deletion attenuated vaccine strain is constructed by deleting a ku80 gene and a CDC14 gene on the basis of a toxoplasma gondii type II PLK strain, and is named as PLK [delta] TgKu80 [delta] TgCDC14 ([delta] CDC14 for short), and experimental results show that the pathogenicity of the strain is remarkably reduced after the CDC14 gene is knocked out. In order to further explore the immune protection effect on toxoplasma gondii acute infection mice, secondary acute infection is carried out on mice immunized with delta CDC14 strains, and the result shows that compared with non-immunized group mice, no immunized mice die within 30 days, and all non-immunized mice die within 13 days. Therefore, the double-gene deletion attenuated vaccine strain has the potential of being used as a vaccine, and an effective technical means is provided for preventing toxoplasma gondii infection.

The invention belongs to the technical field of biological medicine, and discloses a toxoplasma gondii in-vitro merozoite induction model and a construction method thereof. The invention discloses application of a glutamine antagonist to induction of differentiation of toxoplasma gondii tachyzoite to merozoite or preparation of a product for induction of differentiation of toxoplasma gondii tachyzoite to merozoite, and finds that the glutamine antagonist JHU083 can induce differentiation of toxoplasma gondii virulent strain RH and attenuated strain ME49 tachyzoite into merozoite under in-vitro conditions for the first time. A reliable in-vitro model is provided for researching the key initial stage of sexual reproduction of the toxoplasma gondii, and an irreplaceable research foundation is laid for the key link of targeted blocking of parasite transmission.

Provided herein are microbial oils with reduced saturated fatty acids and improved cold flow properties and methods of producing same. The methods include culturing oil-producing microorganisms in a fermentation medium in the presence of one or more antifoaming agents under a controlled carbon consumption rate, wherein the culturing produces oils comprising fatty acids and wherein less than 35% of the fatty acids in the oil are saturated fatty acids.

The invention discloses protozoa zopoda and application of the protozoa zopoda in promotion of tomato growth and prevention and control of fusarium wilt. The protozoa zopoda cometa NJAU-S1 is preserved in the China Center for Type Culture Collection, and the preservation number of the protozoa zopoda cometa NJAU-S1 is CCTCC (China Center for Type Culture Collection) NO: C2025398. Pot experiment results show that inoculation of protozoa nematopoda NJAU-S1 can significantly promote tomato growth, and plate experiments show that inoculation of protozoa nematopoda NJAU-S1 can inhibit growth of tomato fusarium oxysporum and reduce the infection ability of tomato fusarium oxysporum to tomato, so that the inhibition effect of tomato fusarium oxysporum on tomato growth is relieved. The invention provides a new strategy and resource for biological prevention and control of tomato fusarium oxysporum and scientific planting of tomatoes, and has important application value for safe production of food crops.

The invention provides non-naturally occurring microbial organisms containing enzymatic pathways and / or metabolic modifications for enhancing carbon flux through acetyl-CoA. In some embodiments, the microbial organisms having such pathways also include pathways for generating reducing equivalents, formaldehyde fixation and / or formate assimilation. The enhanced carbon flux through acetyl-CoA, in combination with pathways for generating reducing equivalents, formaldehyde fixation and / or formate assimilation can, in some embodiments, be used for production of a bioderived compound. Accordingly, in some embodiments, the microbial organisms of the invention can include a pathway capable of producing a bioderived compound of the invention. The invention still further provides a method for producing a bioderived compound of the invention.

This invention discloses a strain of Acanthamoeba that inhibits the pathogen of Fusarium graminearum and its applications. Acanthamoeba (Acanthamoeba rhysodes NJAU-ZY1, deposited at the China Center for Type Culture Collection (CCTCC) with accession number CCTCC NO: C2025219, demonstrates in plate experiments, liquid co-culture experiments, and pot experiments that inoculation with the protozoan Acanthamoeba NJAU-ZY1 significantly inhibits the growth of Fusarium graminearum pathogen, reduces its infectivity in wheat, alleviates the inhibitory effect of Fusarium graminearum on wheat growth, reduces the risk of diseases caused by this pathogen, and thus increases wheat biomass. This provides a new strategy and resource for the biological control of Fusarium graminearum pathogen and the scientific cultivation of wheat, and has important application value for the safe production of food crops.

The invention discloses a toxoplasma gondii gene deletion strain ME49 [delta] TGZS and an application thereof in preparation of a gene engineering inactivated vaccine. The toxoplasma gondii gene deletion strain ME49 [delta] TGZS is prepared by taking a toxoplasma gondii strain ME49 as a parent strain and knocking out a TGZS gene in the toxoplasma gondii strain ME49; the nucleotide sequence of the TGZS gene is as shown in SEQ ID NO: 1. The strain can maintain normal polypide growth and replication capacity under an in-vitro culture condition, after inactivation treatment, the strain loses proliferative activity but maintains a complete polypide morphological structure, and the strain is compatible with an aluminum hydroxide adjuvant to prepare a vaccine preparation. According to the vaccine, by activating the specific immune response of a terminal host cat, the sexual reproduction cycle of the toxoplasma gondii in the intestinal epithelial cells of the cat is effectively blocked, and the formation and discharge of oocysts are remarkably inhibited, so that the propagation chain of the toxoplasma gondii is controlled from the source. The invention provides a new effective means for prevention and control of toxoplasmosis.

The present invention provides an apparatus, method, and program for extracting multiple images of the same microorganism from multiple captured images. [Solution] In the system 10, the discrimination device 60 includes an acquisition unit that receives multiple images of the flow channel 24 through which microorganisms in the flow channel device 20 flow, and an extraction unit that extracts multiple images of the same individual microorganism from the multiple images. The flow channel through which microorganisms flow may have a vortex generator that generates vortices in the imaging range 26 that captures multiple images within the flow channel. The acquisition unit receives multiple images of the flow channel through which microorganisms flow, taken at different timings. The acquisition unit also receives multiple images taken from two or more different directions.

One aspect of this disclosure is a method for maintaining a plankton population in a culture medium by removing particles from the culture medium. The method may comprise rotating a filter body to lift the particles from the culture medium with a filter of the filter body, positioning the filter relative to a conduit so that a first portion of the lifted particles fall into the conduit, directing a removal fluid toward the filter body to move a second portion of the lifted particles off the filter and into the conduit with impact forces applied by the removal fluid, and / or outputting an effluent flow from the conduit. The effluent flow may comprise the first and second portions of the lifted particles and a portion of the removal fluid. Aspects of related apparatus, methods, and systems also are disclosed.

The application provides a strain of protozoan nephrospora NJAU-HN1 and an application thereof, and belongs to the technical field of agricultural microorganisms. The nephrospora NJAU-HN1 has a preservation number of CCTCC NO: C2025172. The nephrospora NJAU-HN1 can directly and effectively inhibit the survival of root-knot nematodes. Application of the nephrospora NJAU-HN1 or a culture thereof can significantly promote the growth of melon plants, increase the fresh weight, dry weight and root length of the plants. The nephrospora NJAU-HN1 provided by the application provides valuable microbial resources for the development of a new microbial preparation for green prevention and control of melon root-knot nematode disease and crop health promotion, and has a good application prospect.

The invention belongs to the technical field of protozoa preservation. Relates to a cryopreservation and resuscitation method of rumen ciliates. Comprising the following steps: preparing a special cryopreservation culture medium; gathering insect bodies through centrifugation; mixing the cryopreservation protection liquid with the enriched polypide according to a ratio, and slowly and preliminarily freezing in a refrigerator at the temperature of 80 DEG C below zero; transferring into liquid nitrogen for long-term cryopreservation after staying overnight; during resuscitation, preparing a resuscitation culture medium, taking out the cryopreservation tube from the liquid nitrogen, and quickly placing the cryopreservation tube in a water bath at 39 DEG C for unfreezing; centrifugally washing to remove the cryopreservation protective agent and re-enriching the polypide; and inoculating the polypide into a resuscitation culture medium, and culturing in a constant-temperature incubator at 39 DEG C. A standardized cryopreservation resuscitation process of the internal hair rumen ciliates is established, and the operation is simple, convenient and rapid; the survival rate of the resuscitated ciliates is remarkably improved, and the highest survival rate of the mixed species can reach 58.1% through verification; particularly, the highest survival rate of a single species of incarnaeus incarnaeus can reach 66.4%; the resuscitated ciliates can quickly recover and proliferate; the method is simple, rapid and good in repeatability.

The present disclosure features methods for modulating the production of small halogenated organic compounds using peroxidase enzymes, as well as related compositions and methods of use. The present disclosure features peroxidase enzymes and related compositions, as well as methods for modulating the production of small halogenated organic compounds using peroxidase enzymes. In one embodiment, the methods described herein include (i) providing a small organic compound (e.g., acetone or acetylacetone), (ii) contacting the small organic compound with a peroxidase (e.g., VHPO) to form a reaction mixture under conditions sufficient to produce the small halogenated organic compound, and / or (iii) evaluating the amount of halogenated organic compound produced.

The invention is applicable to the technical field of biological detection, and provides a specific antibody and application thereof in detection and enrichment of oocysts of cryptosporidium. According to the method, an immunomagnetic separation detection system is constructed by taking oocyst outer wall protein (cgd6660) as a target: an antibody capable of being specifically combined with the protein is subjected to purification and biotinylation and then is coupled to the surface of a streptavidin magnetic bead, so that efficient and specific capture of the oocyst is realized. The system is efficient and specific in combination with oocysts of cryptosporidium, interference of impurities such as algae and bacteria can be effectively avoided, and the system has good applicability and stability in complex water quality samples with high turbidity, high organic matter and the like. The method is high in process standardization degree and short in operation time, and the autonomously prepared local antibody greatly reduces import dependence and detection cost; by combining fluorescent staining and microscopic confirmation, sensitive and accurate detection of oocysts can be realized, the method is suitable for scenes such as drinking water source monitoring and the like, and stable and reliable technical support is provided for water safety and cryptosporidiosis prevention and control.

The invention discloses an eimeria gene editing system, a recombinant coccidium vector and application of the recombinant coccidium vector. The invention firstly provides the sgRNA targeting the ETH00010755 gene and the gene editing system containing the sgRNA, and the sgRNA and the gene editing system can realize high-efficiency and high-specificity gene editing. A donor plasmid template containing His4 and EtU6 promoters is constructed, compared with an original plasmid template, the donor plasmid template has the advantages that the positive rate is higher, an exogenous gene can be accurately inserted into an ETH00010755 gene, the unspecific gene editing risk can be avoided through the gene editing plasmid, and the high editing efficiency and the low off-target rate are achieved. The gene editing system, the recombinant coccidiosis vector and the construction method of the recombinant coccidiosis vector are mature, reliable and easy to operate and have high success rate and repeatability, and the obtained recombinant coccidiosis vector can be applied to preparation of immune drugs and / or vaccines for preventing eimeria.

The invention relates to the technical field of plasmodiophora brassicae inoculation, in particular to an artificial inoculation method for plasmodiophora brassicae immediate infection and application, and the method specifically comprises the following steps: S1, accelerating germination: accelerating germination of seeds to obtain seedlings; s2, grinding bacteria and preparing a bacteria solution; s3, inoculation: performing field planting on the seedlings obtained in the step S1, and then performing injection inoculation on the overground parts of the seedlings by using the bacterial liquid prepared in the step S2; s4, seedling stage management; s5, disease investigation. According to the method, the cognitive limitation of traditional two-step infection of root hair infection and cortex diffusion is broken through, a brand-new inoculation technical path is constructed, and a new idea and method for inoculating plasmodiophora brassicae of cruciferae are developed; the defects that a traditional underground inoculation method is fuzzy in infection time sequence, uncontrollable in inoculation metering, low in inoculation efficiency and poor in pathogenesis stability due to large bacterial quantity fluctuation are fundamentally overcome.

The present invention concerns a process and related system allowing the improvement of the production / growth of microorganisms at high yield in a culture system comprising a culture medium such as bioreactors or photobioreactors, or plants in various culture systems, by separation of nucleic acids produced by said organisms.

The invention relates to an in-vitro culture method and application of anerennial crabs, and belongs to the technical field of aquaculture. The invention provides a culture medium. The culture medium comprises hemolymph of healthy animals and sterile seawater, the volume ratio of the hemolymph of the healthy animal to the sterile seawater is (1-5): (5-9). According to the in-vitro culture method of the crabbidae, the survival time of the crabbidae inoculated into the culture medium for one time is not less than 1 month under the culture system, and when the crabbidae body reaches the optimal state, the culture is transferred to the condition of 2-8 DEG C, so that the survival time of the crabbidae body can be prolonged to be not less than 2 months, and the survival time of the crabbidae body can be prolonged to be not less than 2 months. If the strains are continuously transferred to a fresh culture medium, long-term stable passage of the polypide can be realized, the in-vitro culture system can effectively guarantee supply of the polypide in non-epidemic seasons, and a key technical support is provided for accelerating research and development of quasi-amarerenosis drugs and realizing effective prevention and control of the quasi-amarerenosis.

Provided herein are eukaryotic microorganisms having a simple lipid profile comprising long chain fatty acids (LCFAs). Also provided are compositions and cultures comprising the eukaryotic microorganisms as well as methods of using the eukaryotic microorganisms.

The invention relates to the technical field of bioengineering, and discloses a specific identification primer for lactobacillus brevis in human intestinal tracts, a detection method and application. The invention provides a specific primer COG4986F3 / COG4986R3 for identifying lactobacillus brevis, and a conventional PCR (Polymerase Chain Reaction) and real-time fluorescent quantitative qPCR (Quantitative Polymerase Chain Reaction) detection method is established; the primer is designed on the basis of the specific COG4986 gene of lactobacillus brevis, is high in specificity and can accurately recognize a target strain under the background of complex flora in the intestinal tract of a human body; the detection method is easy and convenient to operate, high in amplification efficiency and low in detection limit, qualitative detection can be achieved through conventional PCR, and quantitative analysis can be achieved through qPCR; according to the method, an identification system of the lactobacillus brevis is expanded, a precise tool is provided for intestinal microecology research, food microorganism detection and probiotic preparation quality control, and the method is suitable for detection of human intestinal samples, food and environment samples and has important application value.

This invention relates to the field of biotechnology, specifically to a scutocilius membrane protein vaccine, its preparation method, and its application. The specific technical solution is as follows: a method for preparing a scutocilius membrane protein vaccine involves mixing scutocilius membrane protein with an adjuvant. The scutocilius membrane protein is extracted from the scutocilius membrane using ultracentrifugation. This invention prepares an immunogenic vaccine by extracting and purifying scutocilius membrane protein for the prevention or treatment of infections caused by scutocilius.

The invention relates to the field of sewage treatment and the field of microbial mucus preparation, in particular to ciliate biological mucus and an obtaining method and application thereof. The biological mucus of the ciliates obtained through the method is high in sustainability, the ciliates can be cultured on a large scale, and in order to further improve the synchronous secretion efficiency, the cells are preferably subjected to temperature impact pretreatment at the temperature of 4-25 DEG C before chemical induction. The temperature change interferes with the membrane fluidity and the ion gradient, and the potential disturbance effect is amplified, so that the mucous sacs of most cells in the population are synchronously activated in a short time. According to the process, high-efficiency mucus release can be achieved, cell activity can be maintained within the reasonable dosage and action time range, irreversible damage caused by overstimulation is avoided, the yield of mucus is increased, the mucus components are possibly changed, and the obtained mucus removing nano plastic is better in effect.

The invention discloses an acanthamoeba protozoa for inhibiting fusarium graminearum pathogenic bacteria and application of the acanthamoeba protozoa. The acanthamoeba rhysodes NJAU-ZY1 is preserved in the China Center for Type Culture Collection, and the preservation number of the acanthamoeba rhysodes NJAU-ZY1 is CCTCC (China Center for Type Culture Collection) NO: C2025219. Results of a plate experiment, a liquid co-culture experiment and a pot experiment show that inoculation of the protozoa acanthamoeba NJAU-ZY1 can significantly inhibit the growth of the fusarium graminearum pathogenic bacteria and reduce the infection ability of the fusarium graminearum pathogenic bacteria on wheat, so that the inhibition effect of the fusarium graminearum pathogenic bacteria on the growth of the wheat is relieved, and the growth of the wheat is inhibited. The disease risk caused by the pathogenic bacteria is reduced, so that the wheat biomass is improved, a new strategy and a new resource are provided for biological prevention and control of the fusarium graminearum pathogenic bacteria and scientific wheat planting, and the method has an important application value for safe production of food crops.

The invention provides a protist reniform worm NJAU-HN1 and application thereof, and belongs to the technical field of agricultural microorganisms, and the preservation number of the reniform worm NJAU-HN1 is CCTCC (China Center for Type Culture Collection) NO: C2025172. The kidney-shaped worm NJAU-HN1 can be used for directly and effectively inhibiting the survival of the root-knot nematode; by applying the kidney-shaped worm NJAU-HN1 or the culture thereof, the growth of melon plants can be obviously promoted, and the fresh weight, dry weight and root length of the plants can be increased. The reniform worm NJAU-HN1 provided by the invention provides a valuable microbial resource for developing a novel microbial preparation for green prevention and control of melon root knot nematode disease and healthy growth promotion of crops, and has a good application prospect.

The invention discloses a construction method and application of a toxoplasma gondii attenuated strain RH ncl1, and belongs to the technical field of biological medicines. According to the invention, the TgNCL1 gene is knocked out to obtain the toxoplasma gondii attenuated strain with deletion of the TgNCL1 gene, the in-vitro proliferation speed of the toxoplasma gondii with deletion of the TgNCL1 gene is obviously reduced, and the pathogenicity is obviously reduced; the mouse infected with the TgNCL1 gene deletion strain is neither diseased nor dead, and has high safety to a host. After a mouse is immunized by the toxoplasma gondii attenuated strain with deletion of the TgNCL1 gene, a relatively high humoral immunity and cellular immunity antibody level is generated, a remarkable immune protection effect can be provided for reinfection of high-dose RH, PYS tachyzoites and Pru cysts, and reinfection of toxoplasma gondii can be prevented.