194 results about "Attenuated vaccine" patented technology

The invention relates to an anti-PRV gD (pseudorabies virus gD) protein neutralizing monoclonal antibody, a hybridoma cell and application of the anti-PRV gD protein neutralizing monoclonal antibody. The anti-PRV gD neutralizing monoclonal antibody is prepared by constructing a PRV gD eukaryotic expression vector, transduction the PRV gD eukaryotic expression vector into a CHO (Chinese hamster ovary) cell for expression and then carrying out animal immunization by taking a PRV gD recombinant protein and an attenuated live vaccine as immunogens. The neutralizing monoclonal antibody or hybridoma cell provided by the invention lays a foundation for the development of a rapid detection technology of the pseudorabies virus and the gD protein thereof, has wide research and application values and commercial use values in immunodetection related to the pseudorabies virus, and has important guiding significance for vaccine development based on the pseudorabies virus.

The invention discloses a toxoplasma gondii attenuated vaccine strain RH delta rop67 as well as a construction method and application thereof, and belongs to the technical field of parasitic disease prevention and control and biological product preparation. The attenuated vaccine strain is constructed by performing targeted knockout on the ROP67 gene in a toxoplasma gondii strain RH delta ku80 through a CRISPR / Cas9 mediated gene editing technology. Compared with a wild type strain, the attenuated vaccine strain shows remarkable attenuation characteristic and good immunogenicity. A test result shows that the attenuated vaccine strain can induce a host to generate specific immune response mainly based on cellular immunity, maintains a protection effect on toxoplasma gondii infection in a relatively long immune period, and has a protection effect on tachyzoite infection and a chronic infection stage of toxoplasma gondii strains with different virulence; the survival ability of a host to tachyzoite infection can be improved, and the formation level of cysts in tissues is reduced. The invention provides a technical scheme with long-term immune potential for research and development of toxoplasma gondii attenuated vaccines.

The invention discloses a freeze-drying protection system for an influenza live attenuated vaccine. The freeze-drying protection system is composed of a buffer solution and auxiliary materials. The freeze-dried powder is safe and effective, has a relatively good protection effect on an influenza attenuated strain, is relatively low in virus loss before and after freeze-drying, and has good storage stability.

The invention provides a classical swine fever virus subunit vaccine and a preparation method thereof, the classical swine fever virus subunit vaccine is based on an E2 protein gene and a GP67 signal peptide gene of a CSFV 1.1 strain, a recombinant expression vector is constructed through gene cloning, a classical swine fever virus E2 recombinant protein is obtained through expression of an insect baculovirus system, and the classical swine fever virus subunit vaccine is obtained. And the recombinant protein is combined with a colloidal manganese adjuvant to prepare the swine fever virus subunit vaccine. The hog cholera virus E2 recombinant protein is close to natural E2 protein in structural function, high in reduction degree, high in expression quantity and suitable for vaccine production and application; the vaccine can generate specific humoral immunity and relatively strong cellular immunity, and is beneficial to removal of CSFV in an animal body; meanwhile, the vaccine can be distinguished from attenuated vaccine strain immunization or wild strain infection at the nucleic acid level, can be used for preventing and purifying classical swine fever in an intensive pig farm, and has a considerable application prospect.

The invention discloses a recombinant adenovirus based on 33 protective genes of Brucella, 33 most important protective antigen genes are screened from 89 whole genes of Brucella through a bioinformatics technology, and are recombined into a human type 5 replication-deficient adenovirus vector through a genetic engineering technology. Therefore, the recombinant adenovirus vaccine based on 33 protective genes of Brucella is obtained. The invention effectively overcomes the defects of virus dispersion, virulence recombination, virulence reversion, virulence residue and the like of attenuated vaccine strains such as conventional brucella vaccine strains, gene deletion vaccine strains and the like, is a safe and effective vaccine, has the effect reaching the brucella vaccine evaluation standard formulated by the world animal hygiene organization OIE, and can be applied to prevention of brucellosis.

The invention provides a gntR17 gene deleted strain M5 delta gntR17 as well as a construction method and application thereof, and belongs to the technical field of biology. The M5 delta gntR17 strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation date is July 4, 2024, and the preservation number is CGMCC NO.46061. The M5 delta gntR17 strain is preserved in the China General Microbiological Culture Collection Center (CGMCC). It is found that the gntR17 gene is a related gene affecting the virulence of the brucella, the virulence of the brucella can be weakened by deletion of the gntR17 gene, the safety is improved, the survival ability of the brucella in cells and a mouse body can be remarkably reduced after deletion of the gntR17 gene, and the gntR17 gene has potential application value. Compared with the original brucella M5 strain, the M5 delta gntR17 strain constructed after the gntR17 gene is knocked out has the advantages that the virulence is weakened, the safety is high, the immune protection similar to that of an M5-90 delta 26 vaccine is realized, and the strain can be used as a candidate attenuated live vaccine strain for preventing animal brucellosis.

The present invention relates to a mutant plasmid and a preparation method and application thereof, belonging to the field of plant virology and molecular biology. F R2‑2bPT‑PSTVd 1‑200 Contains CMV Fny The full-length RNA2 sequence of the isolate has TAATAG and PSTVd ​​fragment inserted between positions 2682 and 2683; the nucleotide sequence of the PSTVd ​​fragment is shown in SEQ ID NO.1. Fny Isolate RNA1 and modified PCB301‑RNA3‑ △ The apno plasmid and the mutant plasmid of the present invention are pre-mixed and inoculated. The attenuated vaccine can prevent the potato spindle tuber virus from causing harm and avoid the spread of the virus by aphids in the field. The effect is good and provides vaccine materials and effective measures for field control of PSTVd.

The invention discloses construction and application of a streptococcus suis serotype 2 cps2C gene knockout mutant strain. The invention relates to a streptococcus suis serotype 2 cps2C gene knockout mutant strain 05ZYH33 delta cps2C. A coding gene, from the 31st site to the 1000th site, of the cps2C gene in the strain 05ZYH33 is replaced by a spectinomycin resistance gene cassette SpcR. The mutant strain 05ZYH33 delta cps2C is applied to preparation of a streptococcus suis type 2 attenuated vaccine and a subunit vaccine. The capsule of the mutant strain 05ZYH33 delta cps2C is obviously reduced; in-vitro experiments show that the macrophage phagocytosis resistance of the mutant strain 05ZYH33 delta cps2C is reduced, and animal toxicity test results show that the toxicity of the mutant strain is obviously reduced. The mutant strain provides an important clue for screening of protective antigens of multivalent subunit vaccines, and can be applied to development of S.suis attenuated vaccines and multivalent subunit vaccines.

The present disclosure provides a pseudorabies virus (PRV) expressing a porcine circovirus 2 (PCV2) capsid protein in its envelope, and use thereof. In the present disclosure, an attenuated PRV vaccine strain is used as a vector to express an exogenous immunogen. The exogenous immunogen only replaces an extracellular domain of a non-essential envelope protein of the PRV, while retaining a transmembrane domain and an intracellular domain of an original envelope protein, thereby allowing the expression of one or more exogenous immunogens on the viral envelope without changing genes of other PRV autoimmunogens. During the recombinant PRV particle being recognized by the body's immune system, the host's immune system recognizes all immunogens (exogenous immunogens and PRV autoimmunogens) on the recombinant PRV particle and then initiates an immune response, thus exerting a protective effect of a bivalent vaccine or a polyvalent vaccine based on the recombinant PRV particle.

The invention belongs to but not limited to the technical field of genetic engineering technology, and discloses a construction method and application of a BspE gene deletion strain of Brucella effect protein, the BspE gene deletion strain is constructed by using homologous recombination and SacB reverse screening technology, Brucella abortus A19 vaccine strain liquid is used as a template, PCR amplification is carried out to obtain upstream and downstream homologous arms of BspE gene, and the BspE gene deletion strain is constructed by using the upstream and downstream homologous arms of the BspE gene. The method comprises the following steps: carrying out fusion PCR (Polymerase Chain Reaction) amplification on upstream and downstream homologous arms of a BspE gene subjected to gel recovery, and connecting and transforming an obtained fusion fragment and a pUC19-SacB plasmid to obtain a recombinant plasmid pUC19-delta BspE. The invention relates to a bovine brucella BspE deletion vaccine strain, which is characterized in that a plasmid is obtained, the plasmid is electrically transformed into a bovine brucella A19 competent cell, and the positive transformant is a bovine brucella BspE deletion strain through kanamycin resistance and sucrose pressure screening, PCR (polymerase chain reaction) identification and sequencing, and the deletion vaccine strain improves the levels of antibacterial cytokines and specific brucella antibody IgG, and can be used for preparing the bovine brucella BspE deletion vaccine strain. The Brucella attenuated vaccine strain is a novel Brucella candidate attenuated vaccine strain and has a good market prospect.

The invention discloses application of a yncJ gene in regulation and control of toxicity of salmonella typhimurium, and belongs to the technical field of biology. According to the invention, the function of the yncJ gene is studied by constructing the yncJ gene deleted strain of salmonella typhimurium, and it is found that after the yncJ gene is deleted, the multiplication capacity of the strain in macrophages is significantly reduced; infected mouse experiments show that the lethal ability of the yncJ gene deleted strain to mice is remarkably reduced. Moreover, the conventional biochemical characteristics and the in-vitro growth speed of the yncJ gene deletion strain are not obviously changed, so that the yncJ gene deletion strain has an application prospect in production of attenuated vaccines for preventing and treating salmonella typhimurium.

The invention provides an H9N2 avian influenza virus attenuated strain as well as a preparation method and application thereof, and belongs to the technical field of virus attenuation. The invention provides an attenuated strain of an H9N2 type avian influenza virus. The attenuated strain is obtained by modifying an M2 gene; the modification comprises the following steps: carrying out synonymous mutation on overlapped genes for coding the M1 protein and the M2 protein, replacing adjacent genes subjected to synonymous mutation with genes for coding termination codons, and carrying out deletion mutation on intracellular structural domain genes for coding the M2 protein. The H9N2 type avian influenza virus attenuated strain disclosed by the invention can be used for preparing an H9N2 type avian influenza virus attenuated vaccine. After chickens are immunized with the H9N2 avian influenza virus attenuated vaccine, a high-level hemagglutination inhibition antibody can be induced, no toxin is expelled after the chickens are immunized, safety is high, potential harm to the environment is avoided, and a new strategy is provided for prevention and control of H9N2 avian influenza.

This document provides details regarding the construction of a recombinant African swine fever virus (ASFV) live attenuated vaccine for the prevention of African swine fever (ASF) caused by various viral strains of ASFV such as the highly virulent Georgia 2007 isolate (“ASFV-G”). Exemplary vaccines comprise an ASFV-GΔI177l modified virus, i.e., a recombinant ASFV-G modified by deletion of a portion of the I177L ORF such that the I177L gene is rendered non-functional.

The invention discloses a cryptosporidium parvum AOX gene deleted strain as well as a construction method and application thereof, and the cryptosporidium parvum AOX gene deleted strain disclosed by the invention is obtained by editing an AOX gene of cryptosporidium parvum through a gene editing technology. According to the gene editing technology, the cryptosporidium parvum AOX gene is knocked out by using a CRISPR / Cas9 gene editing technology, so that a cryptosporidium parvum AOX gene deleted strain is obtained; the AOX gene is found to be related to insect toxicity for the first time, and the infection intensity of an AOX gene deleted insect strain in a gamma interferon gene knockout mouse body is remarkably reduced. Research shows that after the AOX gene is deleted from the wild type cryptosporidium parvum IIdA20G1 subtype strain, the strain has the advantage that the toxicity is obviously weakened, and can be used for preparing cryptosporidium parvum attenuated vaccines so as to prevent cryptosporidium parvum infection.

A coxsackievirus B4 strain and application thereof are disclosed. The strain is named KM140-G01 and is preserved in China Center for Type Culture Collection (CCTCC) on Jun. 18, 2023, and the preservation number is CCTCC NO: V202356, the preservation address is Wuhan University, China. The strain is a humanized coxsackievirus B4 wild type single purified strain, and has strong virus replication capacity, good genetic stability and immunogenicity; the strain can be applied to the development of human coxsackievirus B4 vaccine production strains, and is suitable for the development of attenuated coxsackievirus B4 vaccine and inactivated coxsackievirus B4 vaccine; the method can also be used for establishing an infection model on Vero cells and suckling mice, and is used for CVB4 infection and pathogenic mechanism research, CVB4 vaccine evaluation and antiviral drug screening.

There are SARS coronavirus 2 recombinant vectors derived from a GH clade SARS coronavirus 2 Korean isolate, which express distinct reporter genes, and the production method thereof. A full-length clone of a SARS coronavirus 2 Korean isolate or a derivative thereof, according to one embodiment, can be used as a standard material for evaluating the efficacy of therapeutic agents and vaccines in cell lines and animal models while maintaining infectivity and replication capacity when restored to viruses, can be used to develop a large-scale testing method for therapeutic agent development, and can be used to develop attenuated vaccine strains. In addition, a SARS coronavirus 2 recombinant vector derived from a Korean isolate or a derivative thereof, expressing a reporter gene, can be used for high-capacity, rapid drug screening in the development of antibody therapeutic agents and antiviral agents.

The application discloses a PEDV chimeric virus attenuation identification method based on reverse genetic operation and application of screening potential attenuated vaccine, and the PEDV chimeric virus CHM2013-SP BJ , CHM2013- BJ , CHM2013-SP BJ -ORF3 CHM , CHM2013-SP BJ -E CHM , CHM2013-SP BJ -M CHM , CHM2013-SP BJ -N CHM and CHM2013-(S+ORF3) BJ are constructed and rescued, loss-of-function test and gain-of-function test are carried out, and the attenuated chimeric virus CHM2013-SP BJ -ORF3 CHM and CHM2013-SP BJ -M CHM are obtained, and it is disclosed that the S gene of the PEDV variant BJ2011C promotes virus pathogenicity in cooperation with the ORF3, E and M genes, and the ORF3 gene is the most critical.

This invention discloses a candidate strain of *R. anatipestifer* double-gene deletion attenuated vaccine, its construction method, and its application. The candidate strain of *R. anatipestifer* double-gene deletion attenuated vaccine is *R. anatipestifer* strain CH-1. B739_RS01935-B739_RS07625 A candidate attenuated live vaccine strain with double gene deletion, deposited at the China Center for Type Culture Collection (CCTCC) on May 22, 2025, with accession number CCTCC NO: M20251080, at Wuhan University, Wuhan, China, has been deposited on that date. The attenuated live strain constructed in this invention showed no significant impact on the weight gain of ducklings after immunization, demonstrating good safety. Furthermore, the protection rate against challenge with the wild-type virulent RACH-1 strain reached 83.3% after immunization, indicating excellent immunoprotective efficacy.

The invention provides an application of trichostatin A in promotion of RNA virus proliferation, and belongs to the field of chemical medicines. The invention discloses a novel application of trichostatin A. The trichostatin A can effectively promote the-1 Ribosomal Frameshift process of RNA viruses, can effectively promote replication and proliferation of the RNA viruses, can be used for preparing products for promoting proliferation of the RNA viruses and can be used for producing and preparing RNA virus inactivated vaccines or attenuated vaccines, and the application of the trichostatin A in promoting proliferation of the RNA viruses and the application of the trichostatin A in promoting proliferation of the RNA viruses and the application of the trichostatin A in promoting proliferation of the RNA viruses and the application of the trichostatin A in promoting proliferation of the RNA viruses and the application of the trichostatin A in promoting proliferation of the RNA viruses and the application of the trichostatin A in promoting proliferation of the RNA viruses. And the production cost can be effectively reduced, the production period is shortened, and wide application prospects and economic values are achieved.

The use of anti-HPV immunogens and nucleic acid molecules encoding them for the treatment and prevention of RRP is disclosed.SOLUTION: Pharmaceutical compositions, recombinant vaccines comprising the DNA plasmids, and live attenuated vaccines are disclosed, as well as methods of inducing an immune response to treat or prevent RRP.SELECTED DRAWING: Figure 1

The application discloses a Toxoplasma gondii DAHPS gene deletion vaccine strain and application thereof. The application provides a Toxoplasma gondii gene knockout strain, which is obtained by directly knocking out a 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase TgDAHPS gene of the Toxoplasma gondii through a gene editing technology, the gene ID of the TgDAHPS gene is TGGT1_221260, and the nucleotide sequence is shown as SEQ ID NO. 1. The gene deletion strain Δdahps provided by the application can grow normally in vitro, does not reproduce in a host in vivo, and is almost non-toxic. After immunization of the Δdahps strain, the host can be induced to have good immune protection. The Δdahps has the advantages of weak virulence and almost no reproduction in vivo as the attenuated vaccine strain of the Toxoplasma gondii, can improve the resistance of the host to wild Toxoplasma gondii, and can be used for preventing the infection of the Toxoplasma gondii in humans and animals, and has important significance for developing more Toxoplasma gondii vaccines.

The invention discloses a porcine epidemic diarrhea and porcine rotavirus (G9 type) bigeminy attenuated vaccine and a preparation method thereof, and relates to the technical field of veterinary biological products. According to the present invention, dominant genotype strains such as PEDV G2 type KQ01 strain (the preservation number is CCTCC NO: V202005) and PoRV G9 type CH09 strain (the preservation number is CCTCC NO: V202191) are adopted as parent strains, a cell continuous passage method is adopted to carry out passage attenuation, and the virus content, the purity, the specificity, the hereditary stability, the safety and the immunogenicity are systematically inspected during the passage process; and then, carrying out proliferation process research on passage attenuated strains PEDV HB17-R and PoRV JS01-R in a bioreactor, harvesting a cell culture, mixing the two antigens according to a proper ratio, adding a proper amount of a stabilizer, and carrying out a freeze vacuum drying process to obtain the high-purity PoRV-HB17-R / PoRV-HB17-R / PoRV-HB17-R / PoRV-HB17-R / PoRV-JS01-R composite antigen. The bigeminy attenuated vaccine is highly matched with current dominant prevalent strains (PEDV G2 type and PoRV G9 type), is based on a suspension culture technology and has both safety and high efficiency.

The invention discloses a toxoplasma gondii double-gene deletion attenuated vaccine strain as well as a construction method and application thereof. The toxoplasma gondii double-gene deletion attenuated vaccine strain is constructed by deleting a ku80 gene and a CDC14 gene on the basis of a toxoplasma gondii type II PLK strain, and is named as PLK [delta] TgKu80 [delta] TgCDC14 ([delta] CDC14 for short), and experimental results show that the pathogenicity of the strain is remarkably reduced after the CDC14 gene is knocked out. In order to further explore the immune protection effect on toxoplasma gondii acute infection mice, secondary acute infection is carried out on mice immunized with delta CDC14 strains, and the result shows that compared with non-immunized group mice, no immunized mice die within 30 days, and all non-immunized mice die within 13 days. Therefore, the double-gene deletion attenuated vaccine strain has the potential of being used as a vaccine, and an effective technical means is provided for preventing toxoplasma gondii infection.

This invention provides a live attenuated Brucella vaccine strain M5Δ pyrE Its construction methods and applications belong to the field of biotechnology. The M5... pyrE The strain was obtained by knocking out Brucella mesenteriae strain M5. pyrE Genetically constructed and deposited at the China Center for Type Culture Collection. This invention discovers that... pyrE The gene is associated with Brucella virulence; knocking out this gene significantly weakens Brucella virulence and reduces its intracellular and mouse viability. Compared to the existing vaccine strain M5-9026, M5... pyrE The strain exhibits superior immunoprotective effects, effectively inducing humoral and Th1 cellular immune responses, and demonstrates good safety. The M5 strain constructed in this invention... pyrE This strain has promising potential as a candidate strain for a live attenuated vaccine to prevent brucellosis in animals.

The present invention relates to the field of viruses, and specifically to a fish rhabdovirus attenuated vaccine freeze-dried protective agent, vaccine and preparation method thereof. The freeze-dried protective agent includes sucrose, seaweed liquid, PEG, gelatin and casein hydrolyzed protein, and has good heat resistance through compounding of multiple components, has good protective effect when stored at 4°C for a long time, can be used to prepare effective freeze-dried vaccines, and ensures the stability of the product under short-term deviation storage conditions.

This invention relates to the field of biotechnology, and more particularly to a mutant of the Rift Valley fever virus L protein and its applications. The amino acid sequence of the mutant is based on the amino acid sequence shown in SEQ ID NO:1, with mutations made at position 1189 (aspartic acid) and / or position 1205 (tryptophan). Through cryo-electron microscopy structural analysis and in vitro mismatch experiments, this invention has confirmed that both identified key amino acid sites are involved in regulating the synthetic fidelity of viral polymerase: mutation at N1189 decreases the synthetic fidelity of the polymerase, while mutation at W1205 increases it. This invention not only provides a new target for the development of attenuated vaccines but also provides crucial evidence for elucidating the molecular regulatory mechanisms of RNA virus fidelity.

The invention relates to a Pseudomonas plecoglossicida pldB gene deletion mutant strain and a preparation method and application thereof, and belongs to the technical field of microorganisms and the field of algae preservation, the pldB gene of the mutant strain is deleted or inactivated, the nucleotide sequence of the pldB gene is shown as SEQ ID NO: 1, and the GenBank accession number of the Pseudomonas plecoglossicida is CP031146.1. The virulence of the mutant strain is obviously reduced compared with that of a wild strain of pseudomonas plecoglossicida; the strain can induce appropriate immune response of a host and up-regulate expression of anti-inflammatory factors, is beneficial to relieving immune pathological injury, has good attenuated live vaccine potential, and provides a brand new vaccine candidate strain for prevention and control of the visceral white-spot disease of the large yellow croaker.

The invention discloses a riemerella anatipestifer double-gene deletion attenuated vaccine candidate strain, a construction method and application, the riemerella anatipestifer double-gene deletion attenuated vaccine candidate strain is a riemerella anatipestifer CH-1 strain B739RS01935-B739RS07625 double-gene deletion attenuated vaccine candidate strain, the preservation number is CCTCC NO: M20251080, the preservation date is May 22, 2025, and the preservation number is CCTCC NO: M20251080. The strain is preserved in China Center for Type Culture Collection, and the address of the preservation unit is Wuhan University, Wuhan, China. After ducklings are immunized by the low virulent strain, the low virulent strain has no obvious influence on weight proliferation of the ducklings and has good safety. And after the ducklings are immunized, the challenge protection rate on RACH-1 wild virulent strains reaches 83.3%, and the immune protection effect is good.

The present application relates to a kind of carp herpesvirus II type ORF150 gene deletion strain and its preparation method and application, belong to aquatic animal disease prevention and control technical field.The present application carp herpesvirus II type ORF150 gene deletion strain preservation number is CCTCC NO:V202603, is preserved in China typical culture preservation center, and is named carp herpesvirus 2 type CyHV-2-SS24-Δ150-GFP Cyvirus cyprinidallo2 The virulence of the present application carp herpesvirus II type ORF150 gene deletion strain is significantly weakened, but still retains good immunogenicity, can induce the immune fish body to produce immune protection to carp herpesvirus II type.ORF Therefore, the present application carp herpesvirus II type ORF150 gene deletion strain can be used as live attenuated vaccine to carp herpesvirus II type, prevent koi and its variety in aquaculture from being infected by carp herpesvirus II type and lead to hematopoietic organ necrosis disease.

The present disclosure relates to a viral vector having an exogenous RNA segment having a hairpin-like structure, for example, having two or more base pairing regions separated by one or more non-base pairing regions. The exogenous RNA segment may have a secondary structure, minimum free energy, average position entropy, or other properties within a specified range, or have a value similar to one or more hairpin-like structures of a reference wild-type virus. In some examples, the viral vector is a live attenuated vaccine. In some examples, the viral vectors down-regulate a susceptibility gene in a host plant.