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74 results about "Parvovirus" patented technology
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Parvovirus is the common name applied to all the viruses in the Parvoviridae taxonomic family, although it can also be used specifically for members of one of the two Parvoviridae subfamilies, the Parvovirinae, which infect vertebrate hosts. Members of the second subfamily, the Densovirinae, which infect invertebrate hosts, are more commonly referred to as densoviruses. In subfamily Parvovirinae there are eight genera, containing a total of 58 recognized species, while in subfamily Densovirinae there are 5 genera and a total of 21 species. These viruses have small genomes, encoding just two genes, and must rely on the synthetic machinery of their host cell for their own preferential replication. This means that many parvoviruses require host cells to enter S-phase before viral DNA replication can initiate, but they do not encode any gene products that can drive this transition. Parvoviruses overcome this problem in various ways: viruses in many genera simply wait within the cell for it to enter S-phase under its own cell cycle control, which means that they can only infect actively-dividing cell populations. In contrast, the so-called adeno-associated viruses (AAVs) from genus Dependoparvovirus must wait until the cell is co-infected by a helper DNA virus, commonly an adenovirus or herpes virus, which does encode gene products that can drive the cell into S-phase, allowing AAV infection to initiate and out-compete the helper virus. A third strategy is used by human bocavirus 1 (HBoV1) from genus Bocaparvovirus, which appears to invoke a specific DNA-damage response in its host cell that ultimately supports viral DNA amplification and progeny virus production.
Nylon 6 nanofiber virus-removing filtering membrane as well as preparation method and application thereof
The invention provides a nylon 6 nanofiber virus-removing filtering membrane and a preparation method and application thereof.The nylon 6 nanofiber virus-removing filtering membrane comprises a base material and a nylon 6 nanofiber membrane compounded to the surface of the base material, the average pore size of the virus-removing filtering membrane is 20 nm, the virus-removing filtering membrane plays a role in blocking parvoviruses, the surface of the virus-removing filtering membrane is charged, and the surface of the virus-removing filtering membrane is not prone to being damaged. Proteins with the same charges on the surface can be prevented from adhering to the surface of the fiber. In the preparation process of the virus-removing filtering membrane, the nylon 6 nanofibers with a certain diameter range are selected and compounded on the surface of the base material in a stable dispersion state, the uniform microporous virus-removing filtering membrane is prepared, the effect that parvoviruses are blocked and protein molecules are not easily intercepted is achieved, the hydrophilicity of the nylon 6 nanofibers is relatively good, and the virus-removing filtering membrane has a good application prospect. The non-specific adsorption of the filtering membrane on the protein is reduced; the nylon 6 nanofiber virus-removing filtering membrane can be applied to the field of biological medicine products, and has important significance on separation and purification of biological preparations.
Owner:WUHAN WEICHEN TECH CO LTD +1
Swine fever and porcine parvovirus bivalent subunit vaccine and preparation method thereof
ActiveCN121293299AViral antigen ingredientsVirus peptidesClassical swine fever virus CSFVPharmaceutical medicine
The invention discloses a swine fever and porcine parvovirus bivalent subunit vaccine and a preparation method thereof. The vaccine comprises a first recombinant protein encoded by a first gene, a second recombinant protein encoded by a second gene and a pharmaceutically acceptable carrier. The first gene has a sequence as shown in SEQ ID NO: 1 or an increased or reduced sequence thereof. And the second gene has a sequence as shown in SEQ ID NO: 2 or an increased or reduced sequence thereof. An antigen E2 protein of a hog cholera virus (CSFV) and a VP2 protein of a porcine parvovirus (PPV) are taken as double targets, a recombinant SC-E2 protein with a SpyCatcher tag and a recombinant ST-VP2 protein with a SpyTag tag are respectively expressed in insect cells through a recombinant baculovirus vector, double-antigen covalent assembly is realized in vitro, and the constructed bivalent subunit vaccine can be used for simultaneously preventing and controlling two epidemic diseases and has a good application prospect. And the vaccine has the advantages of high safety, strong immunogenicity, high prevention and control efficiency, easiness in large-scale production and the like.
Owner:SUZHOU WOMEI BIOLOGY CO LTD
Primer pair and kit for detecting duck parvovirus and duck dependovirus universal type
ActiveCN116479180BMicrobiological testing/measurementMicroorganism based processesDependovirusBiomedical engineering
The present application relates to a kind of primer pair for detecting duck parvovirus and duck dependent virus universal type and its kit and detection method.The kit includes four duck parvovirus (MDPV, N-MDPV, GPV and N-GPV) and the primer pair of duck dependent virus (DAAV) universal type newly found in recent years, sequence as shown in SEQ ID NO.1-2.The specific primer used in the present application can effectively amplify four duck parvovirus (MDPV, N-MDPV, GPV and N-GPV) and the duck dependent virus (DAAV) newly found in recent years, and can quickly determine whether the above pathogen infection exists in the sample to be tested.Currently, there is no duck parvovirus and duck dependent virus (both belong to parvovirus family parvovirus subfamily dependent parvovirus genus) universal type detection kit related research report at home and abroad, and the establishment of the present application can fill the gap in related fields at home and abroad.
Owner:INST OF ANIMAL HUSBANDRY & VETERINARY FUJIAN ACADEMY OF AGRI SCI
Quadruple TaqMan real-time fluorescent quantitative PCR primer group and probe group for simultaneously detecting cGPV, MDPV, MDGPV and SBDSV and kit thereof
PendingCN120829993AMicrobiological testing/measurementMicroorganism based processesPcr methodFluorescent pcr
The invention relates to a quadruple TaqMan real-time fluorescent quantitative PCR (Polymerase Chain Reaction) primer group and a probe group for simultaneously detecting cGPV (Complementary Glutathione Virus), MDPV (Minimal Disease Papilloma Virus), MDGPV (Minimal Disease Papilloma Virus) and SBDSV (Sequence Broadcast DSV) and a kit thereof. The sequences of the primer group and the probe group are respectively shown as SEQ ID NO.1-12. The research successfully develops and verifies a multiple TaqMan-MGB real-time fluorescent PCR method capable of simultaneously detecting and distinguishing four important waterfowl parvoviruses (cGPV, MDPV, MDGPV and SBDSV). The method has good specificity, repeatability and high sensitivity. Compared with a conventional PCR method, the multiple detection method has the advantages that the clinical waterfowl parvovirus detection rate is remarkably improved, mixed infection can be effectively recognized, and the clinical diagnosis efficiency is greatly improved.
Owner:INST OF ANIMAL HUSBANDRY & VETERINARY FUJIAN ACADEMY OF AGRI SCI
An amino acid sequence with steric hindrance effect and a preparation method thereof, a VP2 protein of recombinant porcine parvovirus and a nucleotide sequence thereof, and a virus-like particle assembled thereby
This invention belongs to the field of bioengineering and virus-like particle (VLP) construction technology, specifically relating to an amino acid sequence with steric hindrance effect and its preparation method, the VP2 protein of recombinant porcine parvovirus and its nucleotide sequence, and the virus-like particles assembled from it. An amino acid sequence with steric hindrance effect, SEQ ID NO: 1, is provided. This invention provides an amino acid sequence with steric hindrance effect, which is fused to the N-terminus of the wild-type VP2 protein to obtain a modified VP2 protein. During the critical phase of the assembly of the modified VP2 protein into VLPs, SEQ ID NO: 1 at the N-terminus, due to its steric hindrance effect, pre-occupies and physically seals the 5-fold pores of the capsid, ensuring that the host background nucleic acid cannot enter the particle interior. Through protein structure modification, nucleic acid entry into the particle interior is physically blocked from the source, resulting in a pure antigen with extremely high hollowing rate.
Owner:HENAN AGRICULTURAL UNIVERSITY
DNA impurities in a composition comprising a parvoviral virion
The current invention relates to nucleic acid impurities in a composition comprising a parvoviral vector. In particular, the current invention shows that DNA impurities are not randomly encapsulated within a parvoviral virion. The invention therefore relates to a method for identifying and quantifying a nucleic acid impurity in a composition comprising a parvoviral vector. Finally, the current invention relates to method of determining whether a composition comprising a parvoviral vector is regarded as clinically pure.
Owner:UNIQURE IP BV
Feline parvovirus monoclonal antibody and application thereof
The application provides a feline parvovirus antibody and application, a heavy chain variable region amino acid sequence of the feline parvovirus antibody 3E8 is shown as SEQ ID NO:1; a light chain variable region amino acid sequence is shown as SEQ ID NO:2.The monoclonal therapy group of the parvovirus antibody obtained in the application has a 60% higher cure rate than the adjuvant therapy group, further indicating that the feline parvovirus monoclonal antibody injection has a better treatment effect on the test cat infected with FPV.The monoclonal antibody has very high neutralization activity through in vitro cell level neutralization test and animal protection test, can specifically treat FPV, and is simple to prepare and convenient to operate, and has great significance for preventing and treating the disease.
Owner:TAIZHOU BIOALLY TECHNOLOGY CO LTD +1
Parvovirus-like drug carrier targeting tumor stem cells and preparation method and application thereof
The application discloses a virus-mimic drug carrier targeting tumor stem cells and a preparation method and application thereof. The drug carrier can improve cell uptake efficiency by simulating physical surface characteristics of a biological system and can directly target tumor stem cells. In addition, the drug carrier can protect healthy tissues due to the targeting property, significantly reduce side effects of high-dose single use of a loaded drug such as PTC209, effectively enhance the response to tumor stem cells, inhibit tumor proliferation and metastasis, and reduce drug resistance of combined use of drugs such as cisplatin.
Owner:PEKING UNIV SCHOOL OF STOMATOLOGY
Construction and application of a recombinant pseudorabies virus strain stably expressing green fluorescent protein
The application provides a construction and application of a recombinant pseudorabies virus strain stably and efficiently expressing green fluorescent protein, in the research, a green fluorescent protein gene (EGFP) is inserted into different positions of a double gene deletion vaccine strain (JS-2012-△gE / gI) of a pseudorabies virus mutant strain by using a homologous recombination method. Finally, it is proved that the recombinant virus JS-2012-△gE / gI-VP1 / 2-EGFP has good genetic stability, and the EGFP gene can also be stably and efficiently expressed, and the growth rate on cells is also comparable to that of the parent virus, so that the VP1 / 2 gene is the best exogenous gene insertion site. Subsequently, the classical swine fever virus E2 gene, the parvovirus VP2 gene, the cap gene of the circovirus type 2 and the VP1 gene of the foot-and-mouth disease virus can be replaced or inserted into the EGFP expression frame in a single or combined manner to construct different types of multi-vaccines, and the multi-vaccines have extremely high research value and platform significance.
Owner:SHANGHAI VETERINARY RESEARCH INSTITUTE CAAS (CHINESE ANIMAL HEALTH & EPIDEMIOLOGY CENTER SHANGHAI BRANCH)
Vaccine prepared from Graisseria parasuis SmpA protein and related tandem protein thereof and application of vaccine
The invention provides a vaccine prepared from Gleisseria parasuis SmpA protein and related tandem protein thereof and application, and belongs to the technical field of veterinary biological products. The invention relates to a vaccine prepared from a Gleisseria parasuis SmpA protein, and further relates to recombinant proteins SmpA-OppA1 and OppA1-SmpA which are expressed in a tandem manner, and recombinant proteins SmpA-PalA and PalA-SmpA which are expressed in a tandem manner, namely SmpA and OppA1 recombinant proteins SmpA-OppA1 and OppA1-SmpA. Experiment results show that the SmpA protein, the SmpA-OppA1 and OppA1-SmpA, and the SmpA-PalA and PalA-SmpA all have the obvious advantage and characteristic of strong immune protection efficacy, and after the SmpA protein, the SmpA-OppA1 and OppA1-SmpA as well as the SmpA-PalA and PalA-SmpA are mixed with an adjuvant, the prepared swine Graisseria subunit vaccine has the advantages of simple process and good safety, can effectively resist the infection of Graisseria parasuis, and lays a foundation for industrial production of the swine Graisseria subunit vaccine.
Owner:HENAN UNIV OF SCI & TECH
AAV vectors produced by insect cells comprising Rep52 and Rep78 coding sequences with differential codon biases
The present invention relates to production of proteins in insect cells whereby repeated coding sequences are used in baculoviral vectors. In particular the invention relates to the production of parvoviral vectors that may be used in gene therapy and to improvements in expression of the viral rep proteins that increase the productivity of parvoviral vectors.
Owner:UNIQURE IP BV
Quantitative PCR primers, probes, kits and applications for simian and cytomegalovirus
PendingCN122256574AMicrobiological testing/measurementMicroorganism based processesPathogenic microorganismGenome
The application discloses quantitative PCR primers, probes, kits and application of monkey parvovirus and cytomegalovirus, relates to the technical field of pathogenic microorganism detection, and is characterized in that through deep alignment analysis on whole genomes of the monkey parvovirus and the monkey cytomegalovirus, two specific target regions specific to the two viruses, highly conservative and low in homology with other viruses are screened out, and two groups of primer probe combinations with high specificity and no mutual interference are designed; based on the two groups of primers and probes, a standardized kit with complete components and accurate proportion is constructed, and the kit comprises core components such as a fluorescence quantitative PCR premix and positive and negative controls; meanwhile, an optimized rapid quantitative detection method is established, through standard sample pretreatment, a precise reaction system, optimization of an amplification program and clear interpretation standards, the detection is completed within 2 hours, the detection lower limit is as low as 5 copies / µL, and the detection effect with 100% specificity is achieved.
Owner:SUZHOU XISHAN BIOLOGICAL TECH
Primer probe combination for detecting parvovirus B19
PendingCN121204313AMicrobiological testing/measurementMicroorganism based processesFluoProbesVp1 gene
The invention discloses a primer probe combination for detecting parvovirus B19. According to the invention, a method for simultaneously detecting double targets of the NS1 gene and the VP1 gene of the B19 virus is established, the detection accuracy is improved, the clinical omission ratio is reduced, meanwhile, the result credibility is increased, and unnecessary confirmation experiments are reduced. Meanwhile, the method using the MGB-TaqMan fluorescent probe has the advantages of rapidness, sensitivity, easiness in operation and the like, the double-target synergism is good, and the clinical reinspection condition can be reduced.
Owner:NAT INST FOR FOOD & DRUG CONTROL
Typing detection system for sewage diarrhea virus
PendingCN120989302AMolecular entity identificationMicrobiological testing/measurementMagnetic beadDiarrheal diseases
The present invention relates to the technical field of virus detection, and discloses a sewage diarrhea virus typing detection system, which comprises a pathogen enrichment unit, an improved polyethylene glycol precipitation method is adopted to carry out pathogen enrichment on a sewage sample; and the nucleic acid extraction unit is used for extracting nucleic acid in the enriched sample by using a magnetic bead extraction method. According to the typing detection system for the diarrhea viruses in the sewage, simultaneous detection and typing of various diarrhea viruses such as norovirus, rotavirus, adenovirus, astrovirus, parareovirus and fiveleaf virus in the sewage can be realized, the detection range is widened, powerful support is provided for comprehensive monitoring of virus pollution conditions in the sewage, and the system is suitable for popularization and application. According to the present invention, the multiple fluorescent RT-PCR technology is adopted, and the efficient sample enrichment and nucleic acid extraction method is combined, such that the detection sensitivity is improved, the low-concentration viral nucleic acid can be detected, and the potential viral threat is not missed;
Owner:青岛国际旅行卫生保健中心
Methods, devices, kits and compositions for detecting tapeworm
PendingEP4671759A2Immunoglobulins against animals/humansBiological material analysisHook wormsAfter treatment
Methods, devices, kits and compositions for detecting the presence or absence of one or more tapeworm coproantigens in a sample are disclosed herein. The methods, devices, kits and compositions of the present invention may be used to confirm the presence or absence of tapeworm in a fecal sample from a mammal and may also be able to distinguish between different tapeworm species and in the presence of one or more infections with helminths (such as roundworm, hookworm, whipworm and heartworm), Giardia and parvovirus. Confirmation of the presence or absence of tapeworm in the mammal may be made, for example, for the purpose of selecting an optimal course of treating the mammal and / or for the purpose of determining whether the mammal has been rid of the infection after treatment has been initiated.
Owner:IDEXX LABORATORIES INC
Modified capsid proteins for enhanced delivery of parvovirus vectors
This invention relates to modified parvovirus capsid proteins with enhanced transduction efficiency, viral vectors comprising the same, and methods of using the same for delivery of nucleic acids to a cell or a subject.
Owner:THE UNIV OF NORTH CAROLINA AT CHAPEL HILL
Egg yolk antibody for cat parvovirus and preparation method thereof
According to the present invention, the FPV-BJ strain (2 # seed virus) is adopted as the immunogen, and is inactivated to prepare the egg yolk antibody, the research results show that the egg yolk antibody with the FPV-HI titer of 1: 2048 can be obtained under the condition of the immunogen concentration of 105.0 TCID50 / ml, the obtained egg yolk antibody is higher than the existing reported level, and the egg yolk antibody can be further prepared into the feeding egg yolk antibody egg yolk powder for pet food, according to the present invention, the egg yolk antibody freeze-drying preparation or the egg yolk antibody injection preparation can be used as the drug, and the egg yolk antibody freeze-drying preparation is optimized, such that the treatment period and the treatment effect of the affected cat using the egg yolk antibody freeze-drying preparation or the egg yolk antibody injection preparation can be significantly improved; the symptom recovery time and the recovery time are shorter than those of sick cats without using the egg yolk antibody, so that the egg yolk antibody injection preparation prepared by the invention can effectively relieve clinical symptoms and greatly shorten the curing time.
Owner:BEIJING SHIHUA KANGYUAN BIOTECHNOLOGY CO LTD +1
Parvovirus ITR-based gene delivery vector system
PCT designated stageWO2025174770A1Microencapsulation basedGenetic material ingredientsGene deliveryNucleotide
The present disclosure provides an AAV-ITR based gene delivery system comprising (i) an AAV-ITR vector comprising a double stranded polynucleotide encoding a gene of interest flanked by ITRs and (ii) a Rep vector comprising an mRNA encoding a Rep protein such as Rep78, wherein both vectors are encapsulated, e.g., in a liposome or LNP. Such a combination of ITR vector with the expression of Rep68 / 78 results in amplification of the AAV-ITR vector and enhanced episomal maintenance of the vector in the cells, which provides the base for sustained transgene expression. The liposomes containing the AAV-ITR vector and Rep vector components of the gene delivery system can be targeted to one or more surface proteins on a targeted cell or tissue. The gene of interest can be, for example, chimeric antigen receptors (CAR), or antibodies. Also provided are pharmaceutical compositions, vectors, lipid compositions, kits, and methods of treatment.
Owner:MODEX THERAPEUTICS INC +6
Combined vaccine for swine erysipelothrix erysipelas, porcine parvovirus and leptospirosis
The present invention relates to a combination of a first vaccine comprising a non-replicating immunogen of porcine erysipelothrix sp. And a non-replicating immunogen of porcine parvovirus and a second vaccine comprising a non-replicating immunogen of leptospira sp. And the first vaccine and the second vaccine are jointly injected into corium layers of a first injection part and a second injection part of the pig at different points to prophylactically treat the pig so as to resist swine erysipelothrix erysipelas infection, porcine parvovirus infection and leptospirosis infection.
Owner:INTERVET INT BV
Construction method of recombinant canine distemper virus strain expressing mink parvovirus VP2 protein
ActiveCN119876270BSsRNA viruses negative-senseViral antigen ingredientsCanine distemper virus CDVGenetic engineering
The application provides a construction method of a recombinant canine distemper virus strain expressing mink parvovirus VP2 protein and relates to the technical field of genetic engineering. The construction method uses a mink canine distemper virus attenuated vaccine CDV3 strain as a basis, clones mink parvovirus VP2 genes into a canine distemper virus genome cDNA, and obtains a recombinant canine distemper virus rCDV3-mVP2 strain capable of simultaneously expressing CDV proteins and MEV VP2 proteins through virus reverse genetic technology, so as to provide a candidate vaccine strain for the research and development of a mink canine distemper and parvovirus enteritis double recombinant live vaccine.
Owner:HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
AAV-mediated expression using a synthetic promoter and enhancer
PendingUS20250381308A1Genetic material ingredientsPharmaceutical non-active ingredientsPromoterBioinformatics
An isolated recombinant parvovirus vector comprising a synthetic enhancer comprising plurality of enhancer sequences operably linked to a promoter, and methods of using the vector, are provided.
Owner:THE UNIVERSITY OF IOWA RESEARCH
Parvovirus compositions and related methods for gene therapy
PCT designated stageWO2026064739A1Viral antigen ingredientsPeptide/protein ingredientsGeneBioinformatics
The present disclosure provides technologies comprising parvovirus compositions, preparations, constructs, and methods for gene therapy.
Owner:CARBON BIOSCIENCES INC
Erythroparvovirus with a modified genome for gene therapy
Disclosed are recombinant virions that have a capsid protein or a variant thereof of erythroparvovirus and a nucleic acid that includes a heterologous nucleic acid.
Owner:SYNTENY THERAPEUTICS INC
A multiplex PCR primer set for simultaneously detecting four pathogens, as well as a detection method and kit thereof
ActiveCN115786587BMicrobiological testing/measurementMicroorganism based processesMultiplexParvovirus
The present invention provides a primer set for multiplex PCR for the simultaneous detection of four pathogens, a detection method, and a kit thereof, and relates to the field of biological detection technology. The present invention has developed and designed a rapid nucleic acid extraction method for four common pathogens in living minks and a primer set for multiplex PCR detection. The primer set includes upstream and downstream primers for detecting mink canine distemper virus, parvovirus, Aleutian virus, and Pseudomonas aeruginosa, respectively. The nucleic acid extraction method provided by the present invention is simple and rapid to operate, and has a good extraction effect; the primer set, detection system, and detection method for multiplex PCR detection are characterized by high efficiency, strong sensitivity, good specificity, and good repeatability, and overcome the cumbersome steps of single-plex PCR, are easy to operate, simple and efficient, and have pioneering significance.
Owner:INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
Sheldrake fibroblast passage cell line as well as preparation and domestication method and application thereof
PendingCN121136917AViral antigen ingredientsMicroorganism based processesFibroblast cell lineTGE VACCINE
The invention belongs to the field of cell biology, and relates to a shelduck fibroblast cell line which is finally obtained by performing primary culture, screening and optimized subculture on shelduck embryos serving as materials. The fibroblasts cultured by the method are slender, fusiform, triangular and irregular, and can be continuously subcultured for at least 82 generations; the shelduck fibroblast cell line is used for culturing waterfowl viruses such as duck plague virus, novel duck reovirus, duck tembusu virus, duck parvovirus and the like for the first time; compared with the existing duck-derived fibroblast culture, the duck-derived fibroblast culture medium has the advantages of high virus content and wide application range. The shelduck fibroblast cell line provided by the invention can provide a large amount of high-quality materials for scientific researches of cell engineering, molecular biology, immunology and the like; the method is used for culturing, separating and identifying poultry viruses, provides raw materials for development and production of poultry attenuated vaccines and inactivated vaccines, can shorten the production period and reduce the production cost, and has certain practical significance for large-scale production of vaccines.
Owner:CHINA INST OF VETERINARY DRUG CONTROL
RAA-LFD primer group and probe for pangolin parvovirus and application of RAA-LFD primer group and probe
PendingCN122060926AMicrobiological testing/measurementMicroorganism based processesDimerFeral animal
The invention relates to an RAA-LFD primer group and a probe for pangolin parvovirus and application of the RAA-LFD primer group and the probe, and relates to the technical field of molecular biological detection. The pangolin parvovirus is detected on the basis of the RAA-LFD primer group and the probe, cross reaction with other common pathogens can be avoided, the problem of false positive caused by primer dimers is avoided, high specificity and sensitivity are achieved, and a method is provided for zoo rapid diagnosis, wild animal protection and the like.
Owner:SOUTH CHINA AGRICULTURAL UNIVERSITY
Proliferation culture method of high-titer mouse parvovirus
The invention relates to the field of biology, in particular to a proliferation culture method of high-titer mouse parvovirus. The method comprises the following steps: centrifuging mouse parvovirus harvest liquid to remove cell debris, taking supernatant, filtering with a filter membrane to remove bacteria, carrying out ultrafiltration concentration on a sample to be concentrated, and then carrying out conventional multiplication culture to obtain high-titer mouse parvovirus liquid. Compared with a traditional'conventional mouse parvovirus multiplication-concentration experiment 'mode, the multiplication culture method has the advantages that the yield of the viruses cannot be reduced, the quantity of the obtained viruses is large, the operation is simple, and the cost is low.
Owner:SHANDONG VOIN BIOTECHNOLOGY CO LTD
Double bifunctional vector for AAV generation
This invention provides nucleic acid constructs for the production of recombinant parvovirus gene therapy vectors. [Solution] A cell comprising one or more nucleic acid constructs, each comprising: a first expression cassette comprising a first promoter operably ligated to a nucleotide sequence encoding mRNA, the translation of which in a cell produces at least one of the parvovirus Rep78 and 68 proteins; a second expression cassette comprising a second promoter operably ligated to a nucleotide sequence encoding mRNA, the translation of which in a cell produces at least one of the parvovirus Rep52 and 40 proteins; a third expression cassette comprising a third promoter operably ligated to nucleotide sequences encoding parvovirus VP1, VP2 and VP3 capsid proteins; and a nucleotide sequence comprising a transgene adjacent to at least one parvovirus reverse terminal repeat sequence.
Owner:UNIQURE BIOPHARMA BV