47 results about "Rotavirus" patented technology

A single-domain antibody (sdAb) which binds to head domain of VP6 derived from a human rotavirus A and has neutralization activity against different the rotavirus A types.

The present invention relates to a combination vaccine comprising the antigens: a) Cryptosporidium parvum gp40; b) inactivated bovine rotavirus; c) inactivated bovine coronavirus; and d) E. coli fimbrial adhesins F5 and F41. In addition, the invention relates to methods for the preparation of the vaccine, methods for vaccinating ruminants against infection by Cryptosporidium parvum, bovine rotavirus, bovine coronavirus and E. coli, and to a kit-of-parts at least comprising a container comprising the at least one antigen according to the invention.

The invention discloses an application of estramustine sodium phosphate (EMP) in preparation of a drug for treating rotavirus, and discovers that the estramustine sodium phosphate (EMP) can significantly inhibit replication of rotavirus and can play an excellent anti-rotavirus effect at a lower IC50; as the biological safety of the EMP is excellent, it is found that the EMP can almost completely inhibit rotavirus replication within the safe concentration, and it is found that the EMP not only significantly inhibits transcription of rotavirus, but also can reduce expression of VP6 protein. Thus, EMP can be used to treat rotavirus.

Disclosed are nucleic acid oligomers, including amplification oligomers and detection probes, for detection of rotavirus target nucleic acid. Also disclosed are methods of specific nucleic acid amplification and detection using the disclosed oligomers, as well as corresponding formulations, reaction mixtures, and kits. Methods of synthesizing the nucleic acid oligomers are also disclosed.

A composition comprising Bidens pilosa or an active compound isolated from the Bidens pilosa for use in treating, inhibiting and / or decreasing the presence of pathogenic gut microbiota in an animal in need thereof is disclosed. The pathogenic gut microbiota are at least one selected from the group consisting of Brachyspira, porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis coronavirus (TGEV) and Rotavirus (RV). The composition is also for use in treating, inhibiting and / or decreasing occurrence of swine diarrhea, swine dysentery, swine viral infection and increasing the percentage of swine carcass and bone weight. In one embodiment, the composition is for use in treating, inhibiting and / or decreasing occurrence of swine dysentery associated with Brachyspira.

This invention provides a PCR primer set and method for detecting bovine rotavirus subtypes, belonging to the field of virus detection technology. The invention designs primers with high specificity and sensitivity for different bovine rotavirus subtypes. The detection method is simple to operate, can greatly shorten the typing and identification time, has high accuracy, and saves costs.

The application provides a pair of mouse monoclonal antibodies 4A5 and 2C12 with specific binding to canine rotavirus VP7 protein, and a colloidal gold detection kit prepared from the mouse monoclonal antibodies, which can detect various epidemic strains and standard strains of canine rotavirus with high sensitivity, has a high coincidence rate with an RT-PCR detection method, and can detect epidemic strains and target strains in different regions, has the advantages of rapidness, simplicity, accuracy, and can meet the urgent needs of the present clinical stage.

The present invention relates to compositions and methods for treating or preventing infectious diseases, particularly infections caused by intracellular parasites such as Cryptosporidium species. The present invention also relates to compositions and methods for treating or preventing viral or bacterial infections, particularly intestinal infections such as those caused by rotavirus and Salmonella species infections. The methods include administering a farnesyl diphosphate farnesyltransferase 1 (FDFT1) inhibitor, such as lapaquistat, and the composition includes a farnesyl diphosphate farnesyltransferase 1 (FDFT1) inhibitor, such as lapaquistat.

The invention discloses a G10 type bovine rotavirus nano antibody and application thereof, and belongs to the technical field of nano antibodies. The invention aims to provide the nano antibody of the G10 type bovine rotavirus. The invention provides a bovine rotavirus nano-antibody. The amino acid sequence of a CDR1 of the nano-antibody is shown as SEQ ID NO.9, the amino acid sequence of a CDR2 of the nano-antibody is shown as SEQ ID NO.10, and the amino acid sequence of a CDR3 of the nano-antibody is shown as SEQ ID NO.11. The invention further provides a preparation method of the bovine rotavirus nano-antibody. And a novel efficient strategy is provided for prevention and control of the bovine rotavirus.

The invention discloses a human-sheep rotavirus gene reassortment attenuated strain LG1 and application of the human-sheep rotavirus gene reassortment attenuated strain LG1. The human-sheep rotavirus gene reassortment attenuated strain LG1 comprises: i) a coding gene of a structural protein VP7 of a human G1 type rotavirus HN15D2 strain; and ii) coding genes of structural proteins VP1, VP2, VP3, VP4 and VP6 and non-structural proteins NSP1, NSP2, NSP3, NSP4 and NSP5 of the sheep rotavirus strain LLR. The human-sheep rotavirus gene reassortment strain which takes a sheep rotavirus strain as a skeleton, contains a human G1-VP7 gene and can be stably passaged on a Vero cell is successfully constructed for the first time, and the reassortment strain not only has the safety of an animal strain, but also has the immunogenicity of a human G1 type RV, can effectively prevent infection of a human G1 type wild strain, and has a good application prospect. The method has important significance for developing multivalent RV vaccines containing G1 genotypes and developing RV vaccines which are higher in prevention pertinence and provide a better protection effect on Chinese infantile diarrhea.

The application provides application of rotavirus non-structural protein 4 in improving immune function of recombinant rotavirus subunit vaccine, wherein the application is to express the rotavirus non-structural protein 4 gene and rotavirus antigen gene in different surface rings on a norovirus P particle to obtain a recombinant protein, and the recombinant protein is the recombinant rotavirus subunit vaccine. The application improves the immune function of the recombinant rotavirus subunit vaccine by using the NSP4 protein, and parallel expression of the NSP4 gene and the VP8 antigen gene or the NSP4 antigen gene can improve the NSP4 antigen and the VP8 antigen specific IgG antibody titer, improve the neutralization ability of the VP8 antigen to the rotavirus, and improve the protection of the NSP4 antigen and the VP8 antigen to the diarrhea mice induced by the rotavirus SA11 strain.

The invention provides a bovine rotavirus VP7 protein hybridoma cell strain, a monoclonal antibody and application of the monoclonal antibody in antigen detection, and belongs to the technical field of immunodetection. The invention provides a bovine rotavirus VP7 protein hybridoma cell BRV (bovine rotavirus) Mab-VP7-23 strain, and the preservation number of the BRV Mab-VP7-23 strain is CCTCC (China Center for Type Culture Collection) NO: C2025181. The monoclonal antibody generated by the bovine rotavirus VP7 protein hybridoma cell strain BRVMab-VP7-23 can specifically recognize strains of BRV G6, G8 and G10 genotypes, does not have cross reaction with viruses such as bovine viral diarrhea / mucosal disease virus (BVDV) and the like, has good specificity, can be used for exogenous virus detection (cell inspection method) of BRV in bovine virus live vaccines, and also can be used for detecting BRV in bovine virus live vaccines. The kit can also be used for clinical detection and epidemiological investigation of BRV.

The application discloses a G10 type bovine rotavirus nanobody and application thereof, and belongs to the technical field of nanobodies. The application aims to provide a G10 type bovine rotavirus nanobody. The application provides a bovine rotavirus nanobody, wherein the amino acid sequence of CDR1 of the nanobody is shown as SEQ ID NO. 9, the amino acid sequence of CDR2 of the nanobody is shown as SEQ ID NO. 10, and the amino acid sequence of CDR3 of the nanobody is shown as SEQ ID NO. 11. The application provides a novel and efficient strategy for the prevention and control of bovine rotavirus.

The invention discloses a coupling protein as well as a preparation method and application thereof. According to the preparation method disclosed by the invention, in-vitro self-coupling of the rotavirus VP8 protein and the norovirus VP1 protein is realized by utilizing a Spatier-Spytag system, the prepared coupled protein can induce a neutralizing antibody with higher titer aiming at the rotavirus, and the immunogenicity of the norovirus VP1 protein is not influenced; the immunized animal / human body can generate a high-titer specific IgG binding antibody, a serum blocking antibody and a neutralizing antibody aiming at the two viruses. The coupling protein has a wide application prospect in vaccines for resisting infant diarrhea viruses (norovirus and rotavirus).

The application discloses an antibody against rotavirus and application thereof, and relates to the field of antibodies. The antibody against rotavirus disclosed by the application comprises a heavy chain complementarity determining region and a light chain complementarity determining region, the antibody provides an important raw material source for detection of rotavirus, and has good activity and sensitivity.

The invention provides a traditional Chinese medicine preparation for preventing and treating rotavirus diarrhea of calves and lambs and a preparation method thereof, and belongs to the technical field of traditional Chinese veterinary medicines. The traditional Chinese medicine preparation is prepared from the following traditional Chinese medicinal materials in parts by weight: 1 part of pomegranate bark, 1 part of pomegranate seeds, 3 parts of white halloysite, 3 parts of red halloysite, 3 parts of limonite, 1 part of montmorillonite, 1 part of cuttlebone, 2 parts of liquorice, 3 parts of dried ginger and 2 parts of dark malt. The traditional Chinese medicine preparation is prepared by adopting different superfine grinding processes according to the materials of the medicinal materials, the traditional Chinese medicine preparation is convenient to take, and the superfine particle size can effectively improve the absorption and utilization rate of the body to the medicine and reduce the dosage. The cure rate of the traditional Chinese medicine preparation for the rotavirus diarrhea of the young livestock can reach 90%, and the negative conversion rate of the rotavirus can reach 100%. The traditional Chinese medicine preparation has an exact curative effect on the rotavirus diarrhea of the young livestock, contains safe and mild components, has no toxic or side effect on organisms, and can be widely popularized and applied.

The present application relates to the technical field of virus detection, in particular to a primer probe combination for identifying mixed infection pathogens of cattle and application and kit thereof. When identifying cattle pathogens, the nucleotide sequence of the upstream primer of the primer probe combination used is shown in SEQ ID No. 1, the nucleotide sequence of the downstream primer is shown in SEQ ID No. 2, the nucleotide sequence of the probe is shown in SEQ ID No. 3, and the like scheme. The present application can detect and identify bovine epidemic fever virus, bovine herpes virus 4, bovine rotavirus and clostridium welchii through four groups of primer probes. The primer probe combination provided by the present application can identify and diagnose four common pathogens of cattle through a one-tube reaction system by fluorescence quantitative PCR method, and can also identify mixed infection samples, has the advantages of accurate identification, high sensitivity, simple operation, short time consumption and the like, and can provide effective technical means for the diagnosis of cattle infectious disease pathogens and disease prevention in China.

This invention discloses a G6 bovine rotavirus nanobody and its application, belonging to the field of nanobody technology. The purpose of this invention is to provide a G6 bovine rotavirus nanobody. This invention provides a bovine rotavirus nanobody, namely nanobody 5A. The amino acid sequence of CDR1 of nanobody 5A is shown in SEQ ID NO. 9, the amino acid sequence of CDR2 of nanobody 5A is shown in SEQ ID NO. 10, and the amino acid sequence of CDR3 of nanobody 5A is shown in SEQ ID NO. 11. This provides a novel and efficient strategy for the prevention and control of bovine rotavirus.

The invention discloses a G6 type bovine rotavirus nano antibody and application thereof, and belongs to the technical field of nano antibodies. The invention aims to provide the nano antibody of the G6 type bovine rotavirus. The invention provides a bovine rotavirus nano-antibody, the nano-antibody is a nano-antibody 5A, the amino acid sequence of the CDR1 of the nano-antibody 5A is shown as SEQ ID NO.9, the amino acid sequence of the CDR2 of the nano-antibody 5A is shown as SEQ ID NO.10, and the amino acid sequence of the CDR3 of the nano-antibody 5A is shown as SEQ ID NO.11. The invention further provides a preparation method of the bovine rotavirus nano-antibody. And a novel efficient strategy is provided for prevention and control of the bovine rotavirus.

The present invention relates to a method for preparing a reassortant rotavirus, and provides a reverse genetics platform using an inhibitory RNA as selective pressure for the reassortant rotavirus. Through the method, reassortment probability dramatically increases so that a desired reassortant rotavirus can be prepared in a short time and at low cost.

The present invention pertains to a ribonucleic acid (RNA)-based vaccine for immunizing equine subjects, particularly foals, against equine rotavirus. More specifically, the present invention provides for a mRNA vaccine that contains an open reading frame (ORF) encoding an immunogenic or antigenic viral peptide or polypeptide, such as virulence-associated protein A (VapA), which, when administered to an equine subject, will induce a humoral response and / or a cell-mediated immune (CMI) response against a pathogenic equine rotavirus.

The invention provides a kit for rapidly detecting five calf diarrhea related pathogenic microorganisms, which comprises a colloidal gold test strip for ELISA (enzyme-linked immuno sorbent assay) antigen detection, and a monoclonal antibody is dotted on the test strip. Wherein the monoclonal antibody is prepared by immunizing mice with antigen proteins of bovine rotavirus, bovine coronavirus, escherichia coli, cryptosporidium and clostridium perfringens. The kit for rapidly detecting five pathogenic microorganisms related to calf diarrhea provided by the invention has high detection accuracy, can simultaneously detect five pathogenic microorganisms causing calf diarrhea, and does not cause pathogenic microorganism pollution and affect biological safety. And field operation can be performed, detection is performed immediately after sampling, and a result is given in a relatively short time. The kit disclosed by the invention is simple in detection operation and low in requirements on personnel and equipment.

The invention belongs to the field of biomedical treatment, and particularly relates to a rotavirus, enteric adenovirus and norovirus detection kit. A convection amplification primer group in the kit comprises an upstream primer and a downstream primer, each of the upstream primer and the downstream primer comprises a complementary sequence complementary with target nucleic acid and a tailed sequence, and the tailed sequence is located at the 5'end of the complementary sequence and is not complementary with the target nucleic acid; the complementary sequence has a folded sequence, and the folded sequence is complementary with the tailing sequence, so that the primer sequence has a curved hairpin structure; the enterovirus is a norovirus, an enteroadenovirus and a rotavirus. Meanwhile, a primer probe is designed, and a kit for simultaneously detecting multiple enteroviruses is prepared. The kit can avoid non-specific amplification and improve the detection sensitivity.

The present invention relates to compositions and methods for treating or preventing infections, in particular infections by intracellular parasites such as Cryptosporidium spp. The present invention also relates to compositions and methods for treating or preventing viral or bacterial infections, in particular intestinal infections such as those caused by rotavirus and Salmonella spp. Infections. The methods comprise administration of, and the compositions comprise, a farnesyl-diphosphate farnesyltransferase 1 (FDFT1) inhibitor, such as lapaquistat.

The invention provides a detection reagent and a kit for detecting type 1 bovine viral diarrhea virus and type A bovine rotavirus based on a dual RT-ddPCR technology and application, and belongs to the field of molecular biology, the detection reagent comprises: (a) a first primer pair and a first probe for specific amplification of a type 1 bovine viral diarrhea virus target gene; (b) a second primer pair and a second probe for specifically amplifying the A-type bovine rotavirus target gene; (c) reverse transcriptase, DNA (deoxyribonucleic acid) polymerase and ddPCR reaction premix liquid for the dual RT-ddPCR amplification; according to the invention, the absolute quantitative detection of BVDV-1 and BRV-A in a sample can be realized through dual RT-ddPCR in combination with dual-channel fluorescence detection and Poisson distribution analysis; the sensitivity of the method can reach a single copy and is improved by more than 10 times compared with the traditional qPCR, and the method has no non-specific reaction to other common pathogens.

The invention belongs to the technical field of medical biology, and particularly relates to a circular RNA (Ribonucleic Acid) molecule and an application thereof in resisting rotavirus replication, and provides the circular RNA molecule of which the nucleotide sequence is shown as SEQ ID No.1. Through a high-throughput sequencing technology, a human rotavirus wild strain obtained by separation is infected with a host cell and then is subjected to complete transcription sequencing, so that a high-throughput sequencing result is obtained. A brand-new circular RNA molecule circRNA46 for regulating and controlling rotavirus proliferation is found, the nucleotide sequence of the circular RNA molecule circRNA46 is shown as SEQ ID No.1, the circRNA46 is up-regulated after virus infection, overexpression of the circRNA46 can obviously inhibit virus replication, and the circRNA46 has the effect of resisting rotavirus proliferation.

The application discloses a quadruple RT-qPCR method for simultaneously detecting canine digestive tract viruses, a kit and application, and the real-time fluorescent quantitative PCR detection kit comprises PCR primer probes, the PCR primer probes comprise forward PCR amplification primers, reverse PCR amplification primers and detection probes respectively for canine parvovirus, canine coronavirus, canine adenovirus type 1 and canine rotavirus; the sequences are shown in SEQ ID NO. 1 to SEQ ID NO. 12, respectively, the application can detect canine parvovirus, canine coronavirus, canine adenovirus type 1 and canine rotavirus, has very high sensitivity and specificity, and is favorable for early diagnosis or treatment of sick dogs.

The present invention belongs to the technical field of mRNA vaccine preparation, and relates to a rotavirus multi-antigen mRNA vaccine and its application. The mRNA vaccine comprises a delivery vector and an mRNA immunogenic composition, wherein the composition comprises: i) an mRNA encoding a G1 rotavirus antigen protein VP7 antigenic fragment, the sequence of which is shown in SEQ ID NO: 2; ii) an mRNA encoding a G3 rotavirus antigen protein VP7 antigenic fragment, the sequence of which is shown in SEQ ID NO: 4; and iii) an mRNA encoding a G9 rotavirus antigen protein VP7 antigenic fragment, the sequence of which is shown in SEQ ID NO: 6. The mRNA vaccines prepared by the present invention have been shown to stimulate neutralizing antibody titers through humoral immunity experiments. The multi-antigen mRNA vaccine under compounding can produce higher antibody titers and has a cross-neutralizing effect on different types of rotavirus.

Disclosed is a pharmaceutical agent for the prevention and / or elimination of the adverse effects of the rotavirus NSP4 viral protene in cells and / or organisms using the Lactobacillus plantarum bacteriocin PlnA.

The application discloses an antibody against rotavirus and application thereof, and relates to the field of antibodies. The antibody against rotavirus disclosed by the application comprises a heavy chain complementarity determining region and a light chain complementarity determining region, the antibody provides an important raw material source for detection of rotavirus, and has good activity and sensitivity.