26 results about "Rotavirus" patented technology

A single-domain antibody (sdAb) which binds to head domain of VP6 derived from a human rotavirus A and has neutralization activity against different the rotavirus A types.

Disclosed are nucleic acid oligomers, including amplification oligomers and detection probes, for detection of rotavirus target nucleic acid. Also disclosed are methods of specific nucleic acid amplification and detection using the disclosed oligomers, as well as corresponding formulations, reaction mixtures, and kits. Methods of synthesizing the nucleic acid oligomers are also disclosed.

This invention provides a PCR primer set and method for detecting bovine rotavirus subtypes, belonging to the field of virus detection technology. The invention designs primers with high specificity and sensitivity for different bovine rotavirus subtypes. The detection method is simple to operate, can greatly shorten the typing and identification time, has high accuracy, and saves costs.

The application provides a pair of mouse monoclonal antibodies 4A5 and 2C12 with specific binding to canine rotavirus VP7 protein, and a colloidal gold detection kit prepared from the mouse monoclonal antibodies, which can detect various epidemic strains and standard strains of canine rotavirus with high sensitivity, has a high coincidence rate with an RT-PCR detection method, and can detect epidemic strains and target strains in different regions, has the advantages of rapidness, simplicity, accuracy, and can meet the urgent needs of the present clinical stage.

The invention discloses a G10 type bovine rotavirus nano antibody and application thereof, and belongs to the technical field of nano antibodies. The invention aims to provide the nano antibody of the G10 type bovine rotavirus. The invention provides a bovine rotavirus nano-antibody. The amino acid sequence of a CDR1 of the nano-antibody is shown as SEQ ID NO.9, the amino acid sequence of a CDR2 of the nano-antibody is shown as SEQ ID NO.10, and the amino acid sequence of a CDR3 of the nano-antibody is shown as SEQ ID NO.11. The invention further provides a preparation method of the bovine rotavirus nano-antibody. And a novel efficient strategy is provided for prevention and control of the bovine rotavirus.

The application discloses a G10 type bovine rotavirus nanobody and application thereof, and belongs to the technical field of nanobodies. The application aims to provide a G10 type bovine rotavirus nanobody. The application provides a bovine rotavirus nanobody, wherein the amino acid sequence of CDR1 of the nanobody is shown as SEQ ID NO. 9, the amino acid sequence of CDR2 of the nanobody is shown as SEQ ID NO. 10, and the amino acid sequence of CDR3 of the nanobody is shown as SEQ ID NO. 11. The application provides a novel and efficient strategy for the prevention and control of bovine rotavirus.

The invention provides a traditional Chinese medicine preparation for preventing and treating rotavirus diarrhea of calves and lambs and a preparation method thereof, and belongs to the technical field of traditional Chinese veterinary medicines. The traditional Chinese medicine preparation is prepared from the following traditional Chinese medicinal materials in parts by weight: 1 part of pomegranate bark, 1 part of pomegranate seeds, 3 parts of white halloysite, 3 parts of red halloysite, 3 parts of limonite, 1 part of montmorillonite, 1 part of cuttlebone, 2 parts of liquorice, 3 parts of dried ginger and 2 parts of dark malt. The traditional Chinese medicine preparation is prepared by adopting different superfine grinding processes according to the materials of the medicinal materials, the traditional Chinese medicine preparation is convenient to take, and the superfine particle size can effectively improve the absorption and utilization rate of the body to the medicine and reduce the dosage. The cure rate of the traditional Chinese medicine preparation for the rotavirus diarrhea of the young livestock can reach 90%, and the negative conversion rate of the rotavirus can reach 100%. The traditional Chinese medicine preparation has an exact curative effect on the rotavirus diarrhea of the young livestock, contains safe and mild components, has no toxic or side effect on organisms, and can be widely popularized and applied.

The present application relates to the technical field of virus detection, in particular to a primer probe combination for identifying mixed infection pathogens of cattle and application and kit thereof. When identifying cattle pathogens, the nucleotide sequence of the upstream primer of the primer probe combination used is shown in SEQ ID No. 1, the nucleotide sequence of the downstream primer is shown in SEQ ID No. 2, the nucleotide sequence of the probe is shown in SEQ ID No. 3, and the like scheme. The present application can detect and identify bovine epidemic fever virus, bovine herpes virus 4, bovine rotavirus and clostridium welchii through four groups of primer probes. The primer probe combination provided by the present application can identify and diagnose four common pathogens of cattle through a one-tube reaction system by fluorescence quantitative PCR method, and can also identify mixed infection samples, has the advantages of accurate identification, high sensitivity, simple operation, short time consumption and the like, and can provide effective technical means for the diagnosis of cattle infectious disease pathogens and disease prevention in China.

This invention discloses a G6 bovine rotavirus nanobody and its application, belonging to the field of nanobody technology. The purpose of this invention is to provide a G6 bovine rotavirus nanobody. This invention provides a bovine rotavirus nanobody, namely nanobody 5A. The amino acid sequence of CDR1 of nanobody 5A is shown in SEQ ID NO. 9, the amino acid sequence of CDR2 of nanobody 5A is shown in SEQ ID NO. 10, and the amino acid sequence of CDR3 of nanobody 5A is shown in SEQ ID NO. 11. This provides a novel and efficient strategy for the prevention and control of bovine rotavirus.

The invention discloses a G6 type bovine rotavirus nano antibody and application thereof, and belongs to the technical field of nano antibodies. The invention aims to provide the nano antibody of the G6 type bovine rotavirus. The invention provides a bovine rotavirus nano-antibody, the nano-antibody is a nano-antibody 5A, the amino acid sequence of the CDR1 of the nano-antibody 5A is shown as SEQ ID NO.9, the amino acid sequence of the CDR2 of the nano-antibody 5A is shown as SEQ ID NO.10, and the amino acid sequence of the CDR3 of the nano-antibody 5A is shown as SEQ ID NO.11. The invention further provides a preparation method of the bovine rotavirus nano-antibody. And a novel efficient strategy is provided for prevention and control of the bovine rotavirus.

The present invention pertains to a ribonucleic acid (RNA)-based vaccine for immunizing equine subjects, particularly foals, against equine rotavirus. More specifically, the present invention provides for a mRNA vaccine that contains an open reading frame (ORF) encoding an immunogenic or antigenic viral peptide or polypeptide, such as virulence-associated protein A (VapA), which, when administered to an equine subject, will induce a humoral response and / or a cell-mediated immune (CMI) response against a pathogenic equine rotavirus.

The invention belongs to the field of biomedical treatment, and particularly relates to a rotavirus, enteric adenovirus and norovirus detection kit. A convection amplification primer group in the kit comprises an upstream primer and a downstream primer, each of the upstream primer and the downstream primer comprises a complementary sequence complementary with target nucleic acid and a tailed sequence, and the tailed sequence is located at the 5'end of the complementary sequence and is not complementary with the target nucleic acid; the complementary sequence has a folded sequence, and the folded sequence is complementary with the tailing sequence, so that the primer sequence has a curved hairpin structure; the enterovirus is a norovirus, an enteroadenovirus and a rotavirus. Meanwhile, a primer probe is designed, and a kit for simultaneously detecting multiple enteroviruses is prepared. The kit can avoid non-specific amplification and improve the detection sensitivity.

The present invention relates to compositions and methods for treating or preventing infections, in particular infections by intracellular parasites such as Cryptosporidium spp. The present invention also relates to compositions and methods for treating or preventing viral or bacterial infections, in particular intestinal infections such as those caused by rotavirus and Salmonella spp. Infections. The methods comprise administration of, and the compositions comprise, a farnesyl-diphosphate farnesyltransferase 1 (FDFT1) inhibitor, such as lapaquistat.

The invention provides a detection reagent and a kit for detecting type 1 bovine viral diarrhea virus and type A bovine rotavirus based on a dual RT-ddPCR technology and application, and belongs to the field of molecular biology, the detection reagent comprises: (a) a first primer pair and a first probe for specific amplification of a type 1 bovine viral diarrhea virus target gene; (b) a second primer pair and a second probe for specifically amplifying the A-type bovine rotavirus target gene; (c) reverse transcriptase, DNA (deoxyribonucleic acid) polymerase and ddPCR reaction premix liquid for the dual RT-ddPCR amplification; according to the invention, the absolute quantitative detection of BVDV-1 and BRV-A in a sample can be realized through dual RT-ddPCR in combination with dual-channel fluorescence detection and Poisson distribution analysis; the sensitivity of the method can reach a single copy and is improved by more than 10 times compared with the traditional qPCR, and the method has no non-specific reaction to other common pathogens.

The application discloses a quadruple RT-qPCR method for simultaneously detecting canine digestive tract viruses, a kit and application, and the real-time fluorescent quantitative PCR detection kit comprises PCR primer probes, the PCR primer probes comprise forward PCR amplification primers, reverse PCR amplification primers and detection probes respectively for canine parvovirus, canine coronavirus, canine adenovirus type 1 and canine rotavirus; the sequences are shown in SEQ ID NO. 1 to SEQ ID NO. 12, respectively, the application can detect canine parvovirus, canine coronavirus, canine adenovirus type 1 and canine rotavirus, has very high sensitivity and specificity, and is favorable for early diagnosis or treatment of sick dogs.

The invention relates to the technical field of immunofluorescence detection, and provides a method for detecting rotavirus in vitro based on an immunofluorescence method, and a fixing and permeation integrated composition, a weak acid region anti-quenching sealing liquid and a detection kit which are matched with the method. According to the method, paraformaldehyde and a nonionic surfactant are compounded, so that fixation and permeation synergism is realized, and cell morphology maintenance and antigen epitope accessibility are both considered; and a phosphoric acid-citric acid buffer system is combined with glycerol, and the pH is regulated to a sealing liquid in a weak acid region, so that fluorescence quenching is delayed, and the signal-to-noise ratio and repeatability are improved. According to the invention, the non-specific background is reduced, the observable time is prolonged, and the method is suitable for in-vitro rotavirus immunofluorescence detection.

The present disclosure relates to the field of livestock farming and specifically pertains to an alkaline mineral composition, a method of preparation, and use thereof. The alkaline mineral composition includes a metal ion complex, a mineral ion complex, an alkaline soluble rare earth salt, and a composite gel as active ingredients, formulated in specific mass ratios. It can be used as a feed additive, added to drinking water, or directly fed to animals. The components of the alkaline mineral composition in the present disclosure synergistically enhance efficacy, collectively inhibiting replication and transmission of viruses including coronaviruses, circoviruses, rotaviruses, arteriviruses, and herpesviruses in livestock. It enhances the body's toxin-filtering capacity, strengthens host cell antiviral and immune capabilities, and helps the host resist the damage caused by viruses to the body, effectively improving the viral infection in livestock. It can serve as a safe and effective alternative to antiviral vaccines.

The invention belongs to the technical field of biology, and particularly relates to a rotavirus VP6 protein nano antibody and application thereof. The nano antibody can specifically recognize and bind to the rotavirus VP6 protein, has good affinity with the rotavirus VP6 protein, and can be used for preparing products for diagnosing, preventing or treating diseases or symptoms related to the rotavirus VP6 protein or detecting the existence or level of the rotavirus VP6 protein in a sample.

The invention discloses a primer probe group for detecting sheep rotavirus and application of the primer probe group. The primer probe group comprises an upstream primer with a nucleotide sequence as shown in SEQ ID NO: 1, a downstream primer with a nucleotide sequence as shown in SEQ ID NO: 2 and a probe with a nucleotide sequence as shown in SEQ ID NO: 3. The primer probe group for detecting lamb rotavirus provided by the invention is high in sensitivity and good in specificity, and can quickly and sensitively detect lamb rotavirus.

This invention discloses the application of an NCOA4 gene / NCOA4 protein expression inhibitor in the preparation of drugs to inhibit rotavirus replication, relating to the fields of biomedicine and antiviral therapy. This invention uses an NCOA4 gene / NCOA4 protein expression inhibitor to prepare drugs to inhibit rotavirus replication, effectively suppressing rotavirus replication by inhibiting NCOA4 gene / NCOA4 protein expression.

Provided herein are nucleic acids, vectors, cells, and systems comprising engineered fusion-associated small transmembrane (FAST) proteins from fusogenic Rotaviruses that can mediate cell-cell membrane fusion and syncytium formation. Also provided herein are liposome nanoparticles and lipid nanoparticles comprising fusogenic Rotavirus-derived FAST proteins and their use for the delivery of genetic cargo to a cell.

The invention provides application of an MNP marker combination for identifying rotaviruses which are common pathogens of diarrhea symptoms, a primer pair combination, a kit and application of the kit. The application comprises the steps that the MNP marker combination is used for identifying the pathogen rotavirus type A for the purpose of non-disease diagnosis, the MNP marker combination comprises at least one of a marker site MNP-1, a marker site MNP-2 and a marker site MNP-3, and nucleotide sequences of the marker site MNP-1, the marker site MNP-2 and the marker site MNP-3 are sequentially shown as SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3 in a sequence table. According to the application, the MNP markers are analyzed and detected by fusing the super-multiplex PCR amplification and high-throughput sequencing technology, tens of thousands of MNP markers of hundreds of samples are subjected to typing at a time, high-throughput and sensitive detection and accurate typing of rotaviruses are achieved, and the kit has the advantages of being small in sample demand amount, accurate in diagnosis result, capable of saving data amount and capable of detecting subtypes.

This invention discloses the application of emetine in the preparation of anti-rotavirus drugs, belonging to the field of biotechnology. The application of emetine in the preparation of anti-rotavirus drugs disclosed in this invention demonstrates that emetine has an anti-biosynthetic effect on RV, exerting its anti-RV effect by inhibiting the expression of the structural protein VP6 gene.

Provided herein are a set of primers and probes for detecting rotavirus. The set of primers and probes includes at least one forward primer selected from the group including SEQ ID NOS: 17, 20, 23, 26, 29, 32, 35, 38, 41, 44, 47, 50, 53, 56, 59, 62, 65, 68, 71, 74, 77, 80, 83, 86, 89, 92, 95, 98, 101, 104, 107, 110, 113, 116, 119, 122, 125, 128, 131, 134, 137, 140, 143, 146, 149, 152, 155, 158, 161, 164, 167, 204, 207, 210, and combinations thereof; at least one reverse primer selected from the group including SEQ ID NOS: 18, 21, 24, 27, 30, 33, 36, 39, 42, 45, 48, 51, 54, 57, 60, 63, 66, 69, 72, 75, 78, 81, 84, 87, 90, 93, 96, 99, 102, 105, 108, 111, 114, 117, 120, 123, 126, 129, 132, 135, 138, 141, 144, 147, 150, 153, 156, 159, 162, 165, 168, 205, 208, 211, and combinations thereof; and at least one probe selected from the group including SEQ ID NOS: 19, 22, 25, 28, 31, 34, 37, 40, 43, 46, 49, 52, 55, 58, 61, 64, 67, 70, 73, 76, 79, 82, 85, 88, 91, 94, 97, 100, 103, 106, 109, 112, 115, 118, 121, 124, 127, 130, 133, 136, 139, 142, 145, 148, 151, 154, 157, 160, 163, 166, 169, 206, 209, 212, and combinations thereof. Also provided herein is a method for detecting rotavirus using the set of primers and probes.

The invention discloses a recombinant protein VP8: CTB for inducing a long-acting rotavirus neutralizing antibody and application of the recombinant protein VP8: CTB. According to the present invention, the soluble expression of the CTB-Spycater003 carrier protein and the VP8-Spytag003 protein is achieved, and the rotavirus recombinant subunit vaccine is assembled through the in vitro coupling; the recombinant subunit vaccine has intact antigenicity and immunogenicity, and can induce mice to generate long-acting and high-titer IgG binding antibodies and broad-spectrum neutralizing antibodies.