18 results about "Anti-diarrhea" patented technology

The application discloses a feed additive for improving intestinal health of animals and preventing and treating intestinal diseases and application thereof, and relates to the technical field of green and healthy breeding. The application firstly confirms that indole-3-butyric acid (IBA) has no toxic effect on cells and can relieve oxidative stress damage and inflammation damage of the cells in an effective concentration. Meanwhile, the application also fills the application blank of IBA as one of indole derivatives in livestock production, and provides experimental basis and theoretical basis for the fact that IBA is expected to be a new green additive for replacing antibiotics and zinc oxide. More importantly, the application also finds that IBA has positive effects in aspects of resisting diarrhea, repairing intestinal barrier damage, relieving intestinal inflammation and preventing and treating intestinal related diseases.

The invention discloses a piglet anti-diarrhea complete feed formula based on a microecological preparation, and relates to the technical field of animal nutrition and health. The microsphere has a unique three-layer core-shell structure, wherein a core layer is coated with butyric acid producing probiotics and precise prebiotics; the middle layer is a chitosan-gallic acid-vanillin terpolymer with dual functions of strong mucous membrane adsorbability and quorum sensing quenching; and the shell layer is a pH-sensitive slow-release coating and is loaded with an acidifying agent. The structure is constructed through a layer-by-layer coating process, and the technical problems that in the prior art, functional components cannot be delivered in a targeted mode, the synergistic effect is poor, and intestinal barriers cannot be actively repaired are solved. When the microspheres enter animal digestive tracts, the shell layer firstly releases an acidifying agent at the front end to inhibit pathogenic bacteria and clear obstacles for subsequent steps; after entering the small intestine, the middle layer polymer is released, adheres and repairs the damaged intestinal mucosa in the form of a biological bandage, and interferes pathogen communication at the same time.

The invention relates to a preparation method of an animal anti-diarrhea feed additive containing delinted moxa powder and essential oil. The preparation method of the animal anti-diarrhea feed additive containing the delinted moxa powder and the essential oil comprises the following steps: mixing essential oil components thymol and cinnamyl aldehyde, and stirring at 35-65 DEG C until the essential oil components are dissolved to obtain an essential oil solution; the preparation method comprises the following steps: mixing antioxidants BHA and PG, citric acid and ethanol, and stirring until dissolving to obtain an ethanol solution; mixing the ethanol solution with stone powder, and drying to remove ethanol to obtain a solid mixture; mixing the solid mixture with the essential oil solution, adding delinted moxa powder, and stirring uniformly; according to the preparation method of the animal anti-diarrhea feed additive containing the delinted moxa powder and the essential oil, stable combination of the essential oil and the delinted moxa powder is achieved, the diarrhea morbidity and mortality are remarkably reduced in animal experiments, and the effect is better than that of independent use of the essential oil.

The invention relates to a low-residual zinc complex feed additive and a preparation method and application thereof, and belongs to the technical field of feed additives. The zinc element in the feed additive exists in the form of a small peptide zinc complex, the chelating strength coefficient of the small peptide zinc complex is not lower than 850, and the zinc retention rate after the small peptide zinc complex is digested in simulated gastric juice for 2 hours is not lower than 95%. The preparation method comprises the following steps: carrying out enzymolysis on protein to obtain a small peptide solution, then carrying out complex reaction on the small peptide solution and zinc salt under the coupling condition of pulse ultrasound and gradient heating, and then carrying out dynamic pH refining and drying. Through a unique coupling technology, the problems that conventional organic zinc gastric juice is poor in stability and poor in effect under the low addition amount are solved, the prepared additive can achieve the anti-diarrhea effect equivalent to that of 1200-1600 mg / kg zinc oxide with the low addition amount of 300-600 mg / kg (in terms of zinc element) in daily ration of weaned piglets, meanwhile, the zinc discharge amount of excrement is reduced by 80% or above, and the production cost is reduced. Unification of efficient replacement and low residue is realized.

The invention provides a preservation method of anti-diarrhea enterococcus faecium which is separated from excrement of a healthy baby, and particularly provides a preservation method of the anti-diarrhea enterococcus faecium which is separated from the excrement of the healthy baby. According to the method, a three-in-one composite preservation system of stress resistance protection, old-age intestinal tract adaptation and functional activity locking is constructed aiming at the adaptive pain point of an infant source strain and old-age intestinal tract micro-ecology, and a gradient cooling-vacuum freeze drying coupling process is combined; according to the present invention, the strain has characteristics of high viable bacteria survival rate, high senile intestinal tract colonization rate and anti-diarrhea function stability during the long-term preservation, and the technical problems that the existing preservation technology neglects the cross-age strain suitability and only pays attention to the viable bacteria number so as to cause poor colonization and function attenuation when the infant-derived anti-diarrhea strain is used for the elderly are solved.

The invention discloses a preparation method and application of a traditional Chinese medicine composition for improving diarrhea, relates to the technical field of traditional Chinese medicine compositions, and solves the problem of poor clinical improvement effect of existing compatibility. The preparation method comprises the following steps: processing rhizoma zingiberis, rhizoma atractylodis macrocephalae, radix glycyrrhizae and fructus gardeniae, adding ginseng, weighing the processed rhizoma zingiberis, rhizoma atractylodis macrocephalae, radix glycyrrhizae and fructus gardeniae, uniformly mixing, crushing, decocting with water for multiple times, filtering with gauze, and merging filtrates to obtain the traditional Chinese medicine composition. The invention can be used for preparing the traditional Chinese medicine preparation for improving diarrhea. The dosage forms of the traditional Chinese medicine preparation comprise granules, oral thick paste, oral liquid, powder, pills, tablets and capsules. The traditional Chinese medicine composition can be applied to prevention and health care of acute and chronic diarrhea and functional dyspepsia, can be intermittently taken by people with long-term spleen deficiency and easy diarrhea at low dosage, and can also be used for combined medication. On the premise of maintaining cell safety, side effects of traditional hormone therapy are avoided through component synergy, and a new direction is provided for developing a novel anti-diarrhea preparation with both safety and high efficiency.

The invention discloses a comprehensive modification method for remarkably improving the anti-diarrhea activity of sodium brown humate, and belongs to the technical field of biological medicines.The method combines a chemical modification technology and a physical modification technology, the anti-diarrhea activity strength of sodium brown humate can be greatly improved and can be improved to three times or above as high as that of sodium brown humate before modification, and the anti-diarrhea activity of sodium brown humate is improved. The activity intensity of the sodium humate can reach more than two times of the activity intensity of commercially available sodium humate; in addition, the original sample without the anti-diarrhea activity can show the activity; the method disclosed by the invention has important application value and practical significance in enhancing the stability of the quality of the medicine raw materials, relieving the limitation on the source of the feed coal, expanding the selection range of the medicine raw materials, improving the safety of the raw materials and solving the bottleneck problem in the actual production of a medicine enterprise. Meanwhile, the comprehensive modification technology is high in production efficiency, high in applicability, high in standardization degree and easy to industrially apply.

The invention belongs to the technical field of pig feed, and relates to a weaned piglet creep feed capable of enhancing immunity and resisting diarrhea and a preparation method of the weaned piglet creep feed. The weaned piglet creep feed comprises basic raw materials such as puffed corn, broken rice, puffed soybeans, fermented soybean meal, soybean milk powder and fish meal, and a creep core material; the core material contains an egg yolk antibody IgY resisting escherichia coli K88 and plant polysaccharide compounded by sweet wormwood herb polysaccharide, inulin polysaccharide and salvia miltiorrhiza polysaccharide in proportion. Through the synergistic effect of the specific egg yolk antibody and the immunocompetence polysaccharide, the immunity of piglets is remarkably enhanced, pathogenic bacteria colonization is inhibited, and the diarrhea rate is effectively reduced. The invention also provides a corresponding preparation method. The preparation method comprises the processes of raw material crushing, segmented mixing and granulation. The creep feed is comprehensive in nutrition and good in digestion and absorption, can promote the growth performance of weaned piglets, and meets the requirements of green and healthy breeding.

The invention provides a feed for improving disease resistance of Songliao black piglets and application of the feed, and belongs to the technical field of healthy breeding of livestock. The feed comprises a basic ration and the compound polysaccharide of the cortex dictamni and the sambucus williamsii, wherein the compound polysaccharide is preferably prepared by mixing and extracting the cortex dictamni and the sambucus williamsii according to the weight ratio of 3: 2. The preparation method of the compound polysaccharide comprises the steps of raw material pretreatment, ultrasonic mixing extraction, deproteinization and 10 kDa and 300 Da membrane separation and purification. Experimental results show that in an enterotoxigenic Escherichia coli challenge model, Songliao black piglets fed with the feed (in a ratio of 3: 2) show significant unexpected synergistic interaction, the diarrhea rate can be significantly reduced, and intestinal barrier injury can be effectively repaired. Therefore, the invention provides an efficient and green anti-diarrhea and growth-promoting solution for the Songliao black piglets, and has important application value.

The present invention relates to mutants of an E. coli heat-stable toxin (ST) having the following wild-type sequence: NSSNYCCELCCNPACTGCY wherein the mutant has two mutations wherein the first mutation is selected from L9K, L9N and L9T and the second mutation is A14T.

The invention discloses a construction method of a polycarbophil calcium tablet enhanced anti-diarrhea self-adaptive release system. According to the method, calcium polycarbophil and an ion-exchangeable multivalent metal salt carrier are treated under controlled humidity to form a microporous structure, so that the calcium polycarbophil and the ion-exchangeable multivalent metal salt carrier can be replaced and released by monovalent cations in intestinal fluid. The obtained pretreated component and a biodegradable porous polymer matrix are mixed and pressed at low temperature, and pH responsive microcapsules are distributed in the matrix. A composite blocking layer cross-linked by hydrophilic and hydrophobic polymers is formed on the surface of the matrix, so that the expansion rate of the composite blocking layer dynamically changes along with local pH; the outer layer is provided with a controllable degradation nano-porous membrane, and the pore size distribution is reconfigured under the induction of the ion concentration gradient. After constant-temperature and constant-humidity compaction treatment, the obtained system shows a self-adaptive release curve in an in-vitro simulation experiment, and delayed effect and stable release can be realized under different individual intestinal tract conditions. According to the method, the treatment effect of the anti-diarrhea medicine is remarkably improved through a multi-response mechanism.

The invention belongs to the technical field of biology, and particularly relates to an antibacterial peptide capable of improving animal intestinal health and resisting diarrhea and a preparation method thereof.The novel antibacterial peptide is prepared, and the amino acid sequence of the novel antibacterial peptide is shown as SEQ ID NO: 1; experiments prove that after the cat is raised in a strange environment for one week, the antibacterial peptide can slightly improve the propionic acid and butyric acid levels of the experimental group cat; and the total BCFAs level of the experimental group before and after transportation is lower than that of the blank group. Although the alpha diversity and the beta diversity between an experimental group fed with the antibacterial peptide and a blank group have no significant difference, the feeding of the antibacterial peptide significantly increases the levels of bacteroides, parabacteroides and paraprevotella in the experimental group, and significantly reduces the levels of harmful bacteria such as salmonella and actinomycetes. Therefore, the antibacterial peptide disclosed by the invention can be used for preparing products for improving animal intestinal health and resisting diarrhea, such as feed additives or oral liquid.

The invention discloses application of single-base nonsense mutation of a sheep FUT2 gene in diarrhea resistance, and belongs to the technical field of diarrhea-resistant breeding of sheep. The invention provides a single base mutation related to diarrhea resistance of sheep, which is located at the 55082566th nucleotide from the 5'end of chromosome 14 of a sheep genome AR-UIRamv2.0 version, and when the site is T, the sheep shows the ability to resist infectious diarrhea; the provided single base mutation is linked with sheep anti-diarrhea, can be used as a molecular marker, is used for breeding infectious diarrhea resistant sheep varieties and / or screening natural anti-diarrhea individuals, is extremely high in accuracy, strong in universality and simple and convenient to operate, can realize fundamental transformation from post phenotype selection to beforehand genotype selection, and has a wide application prospect. The method has important significance in simplifying the breeding process, shortening the breeding period and workload and improving the breeding efficiency.

The application discloses a preparation method of humic acid anti-diarrhea medicine, and belongs to the technical field of biological medicine, which can greatly improve the anti-diarrhea activity intensity of humic acid substances by homogenizing and grading the humic acid substances, and the activity intensity of the prepared anti-diarrhea raw medicine can reach three times of that before grading; in addition, the original inactive samples can also show anti-diarrhea activity, so that the invalid humic acid resources obtain medicinal value. The method systematically realizes the efficient preparation of humic acid anti-diarrhea raw medicine, and has important practical significance and application value for enhancing the stability of the quality of the medicine raw material, expanding the selection range of the medicine raw material, improving the safety of the medicine raw material and solving the bottleneck problem in the actual production of pharmaceutical enterprises. Meanwhile, the system and operability of the whole method are strong, the applicability is high, and the method is easy to be applied in industrialization.

The invention provides a method for preparing an anti-diarrheal disease feed from beer brewing waste and application, and belongs to the technical field of microbial fermentation feeds, and particularly relates to a method for preparing an anti-diarrheal disease feed by mixing 3-4 parts of brewer's grains, 3-4 parts of corn kernels, 2-4 parts of soybean meal, 1-2 parts of beer waste yeast powder, 1-2 parts of rice bran and 1-2 parts of wheat bran as a fermentation raw material. Adding a waste beer yeast washing solution-cyclodextrin solution, a waste beer yeast agent, bacillus subtilis and lactobacillus acidophilus, and carrying out anaerobic solid fermentation to prepare the anti-diarrhea feed. The prepared feed can significantly increase the weight gain of the fattening pigs and reduce the feed-gain ratio and the diarrhea rate.

The present invention relates to mutants of an E. coli heat-stable toxin (ST) having the following wild-type sequence: NSSNYCCELCCNPACTGCY wherein the mutant has two mutations wherein the first mutation is selected from L9K, L9N and L9T and the second mutation is Al4T.

This invention belongs to the field of biotechnology, specifically relating to an antimicrobial peptide for improving intestinal health and combating diarrhea in animals, and its preparation method. The invention prepares a novel antimicrobial peptide with the amino acid sequence shown in SEQ ID NO:1. Experiments have verified that after one week of feeding in an unfamiliar environment, the antimicrobial peptide can slightly increase the levels of propionic acid and butyric acid in the experimental group of cats. Furthermore, the overall BCFAs levels in the experimental group were lower than those in the control group before and after transportation. Although there were no significant differences in α-diversity and β-diversity between the experimental group fed the antimicrobial peptide and the control group, feeding the antimicrobial peptide significantly increased the levels of Bacteroides, Parabacterium, and Prevotella in the experimental group, and significantly reduced the levels of harmful bacteria Salmonella and Actinomyces. Therefore, the antimicrobial peptide of this invention can be used to prepare products for improving intestinal health and combating diarrhea in animals, such as feed additives or oral liquids.

The invention discloses an anti-diarrhea feed additive and a preparation method thereof, and belongs to the technical field of feed additives. The anti-diarrhea feed additive is prepared from 5 to 10 parts of phloretin, 3 to 5 parts of creatine, 10 to 15 parts of methyl cinnamate, 10 to 15 parts of ethyl cinnamate, 10 to 15 parts of propyl cinnamate, 10 to 15 parts of butyl cinnamate, 20 to 30 parts of montmorillonite, 10 to 15 parts of inulin, 6 to 10 parts of hydrogenated vegetable oil and 3 to 5 parts of sodium alginate. According to the anti-diarrhea feed additive and the preparation method thereof, the phloretin, the creatine, the cinnamate, the montmorillonite and the inulin are scientifically compounded, so that the anti-diarrhea effect of the feed additive is remarkably improved, meanwhile, intestinal health can be effectively guaranteed, nutrient absorption is promoted, and the growth performance of livestock and poultry is improved.