9 results about "Anti-diarrhea" patented technology

The application discloses a feed additive for improving intestinal health of animals and preventing and treating intestinal diseases and application thereof, and relates to the technical field of green and healthy breeding. The application firstly confirms that indole-3-butyric acid (IBA) has no toxic effect on cells and can relieve oxidative stress damage and inflammation damage of the cells in an effective concentration. Meanwhile, the application also fills the application blank of IBA as one of indole derivatives in livestock production, and provides experimental basis and theoretical basis for the fact that IBA is expected to be a new green additive for replacing antibiotics and zinc oxide. More importantly, the application also finds that IBA has positive effects in aspects of resisting diarrhea, repairing intestinal barrier damage, relieving intestinal inflammation and preventing and treating intestinal related diseases.

The invention provides a preservation method of anti-diarrhea enterococcus faecium which is separated from excrement of a healthy baby, and particularly provides a preservation method of the anti-diarrhea enterococcus faecium which is separated from the excrement of the healthy baby. According to the method, a three-in-one composite preservation system of stress resistance protection, old-age intestinal tract adaptation and functional activity locking is constructed aiming at the adaptive pain point of an infant source strain and old-age intestinal tract micro-ecology, and a gradient cooling-vacuum freeze drying coupling process is combined; according to the present invention, the strain has characteristics of high viable bacteria survival rate, high senile intestinal tract colonization rate and anti-diarrhea function stability during the long-term preservation, and the technical problems that the existing preservation technology neglects the cross-age strain suitability and only pays attention to the viable bacteria number so as to cause poor colonization and function attenuation when the infant-derived anti-diarrhea strain is used for the elderly are solved.

The invention belongs to the technical field of pig feed, and relates to a weaned piglet creep feed capable of enhancing immunity and resisting diarrhea and a preparation method of the weaned piglet creep feed. The weaned piglet creep feed comprises basic raw materials such as puffed corn, broken rice, puffed soybeans, fermented soybean meal, soybean milk powder and fish meal, and a creep core material; the core material contains an egg yolk antibody IgY resisting escherichia coli K88 and plant polysaccharide compounded by sweet wormwood herb polysaccharide, inulin polysaccharide and salvia miltiorrhiza polysaccharide in proportion. Through the synergistic effect of the specific egg yolk antibody and the immunocompetence polysaccharide, the immunity of piglets is remarkably enhanced, pathogenic bacteria colonization is inhibited, and the diarrhea rate is effectively reduced. The invention also provides a corresponding preparation method. The preparation method comprises the processes of raw material crushing, segmented mixing and granulation. The creep feed is comprehensive in nutrition and good in digestion and absorption, can promote the growth performance of weaned piglets, and meets the requirements of green and healthy breeding.

The invention provides a feed for improving disease resistance of Songliao black piglets and application of the feed, and belongs to the technical field of healthy breeding of livestock. The feed comprises a basic ration and the compound polysaccharide of the cortex dictamni and the sambucus williamsii, wherein the compound polysaccharide is preferably prepared by mixing and extracting the cortex dictamni and the sambucus williamsii according to the weight ratio of 3: 2. The preparation method of the compound polysaccharide comprises the steps of raw material pretreatment, ultrasonic mixing extraction, deproteinization and 10 kDa and 300 Da membrane separation and purification. Experimental results show that in an enterotoxigenic Escherichia coli challenge model, Songliao black piglets fed with the feed (in a ratio of 3: 2) show significant unexpected synergistic interaction, the diarrhea rate can be significantly reduced, and intestinal barrier injury can be effectively repaired. Therefore, the invention provides an efficient and green anti-diarrhea and growth-promoting solution for the Songliao black piglets, and has important application value.

The invention belongs to the technical field of biology, and particularly relates to an antibacterial peptide capable of improving animal intestinal health and resisting diarrhea and a preparation method thereof.The novel antibacterial peptide is prepared, and the amino acid sequence of the novel antibacterial peptide is shown as SEQ ID NO: 1; experiments prove that after the cat is raised in a strange environment for one week, the antibacterial peptide can slightly improve the propionic acid and butyric acid levels of the experimental group cat; and the total BCFAs level of the experimental group before and after transportation is lower than that of the blank group. Although the alpha diversity and the beta diversity between an experimental group fed with the antibacterial peptide and a blank group have no significant difference, the feeding of the antibacterial peptide significantly increases the levels of bacteroides, parabacteroides and paraprevotella in the experimental group, and significantly reduces the levels of harmful bacteria such as salmonella and actinomycetes. Therefore, the antibacterial peptide disclosed by the invention can be used for preparing products for improving animal intestinal health and resisting diarrhea, such as feed additives or oral liquid.

The invention discloses application of single-base nonsense mutation of a sheep FUT2 gene in diarrhea resistance, and belongs to the technical field of diarrhea-resistant breeding of sheep. The invention provides a single base mutation related to diarrhea resistance of sheep, which is located at the 55082566th nucleotide from the 5'end of chromosome 14 of a sheep genome AR-UIRamv2.0 version, and when the site is T, the sheep shows the ability to resist infectious diarrhea; the provided single base mutation is linked with sheep anti-diarrhea, can be used as a molecular marker, is used for breeding infectious diarrhea resistant sheep varieties and / or screening natural anti-diarrhea individuals, is extremely high in accuracy, strong in universality and simple and convenient to operate, can realize fundamental transformation from post phenotype selection to beforehand genotype selection, and has a wide application prospect. The method has important significance in simplifying the breeding process, shortening the breeding period and workload and improving the breeding efficiency.

The application discloses a preparation method of humic acid anti-diarrhea medicine, and belongs to the technical field of biological medicine, which can greatly improve the anti-diarrhea activity intensity of humic acid substances by homogenizing and grading the humic acid substances, and the activity intensity of the prepared anti-diarrhea raw medicine can reach three times of that before grading; in addition, the original inactive samples can also show anti-diarrhea activity, so that the invalid humic acid resources obtain medicinal value. The method systematically realizes the efficient preparation of humic acid anti-diarrhea raw medicine, and has important practical significance and application value for enhancing the stability of the quality of the medicine raw material, expanding the selection range of the medicine raw material, improving the safety of the medicine raw material and solving the bottleneck problem in the actual production of pharmaceutical enterprises. Meanwhile, the system and operability of the whole method are strong, the applicability is high, and the method is easy to be applied in industrialization.

The present invention relates to mutants of an E. coli heat-stable toxin (ST) having the following wild-type sequence: NSSNYCCELCCNPACTGCY wherein the mutant has two mutations wherein the first mutation is selected from L9K, L9N and L9T and the second mutation is Al4T.

This invention belongs to the field of biotechnology, specifically relating to an antimicrobial peptide for improving intestinal health and combating diarrhea in animals, and its preparation method. The invention prepares a novel antimicrobial peptide with the amino acid sequence shown in SEQ ID NO:1. Experiments have verified that after one week of feeding in an unfamiliar environment, the antimicrobial peptide can slightly increase the levels of propionic acid and butyric acid in the experimental group of cats. Furthermore, the overall BCFAs levels in the experimental group were lower than those in the control group before and after transportation. Although there were no significant differences in α-diversity and β-diversity between the experimental group fed the antimicrobial peptide and the control group, feeding the antimicrobial peptide significantly increased the levels of Bacteroides, Parabacterium, and Prevotella in the experimental group, and significantly reduced the levels of harmful bacteria Salmonella and Actinomyces. Therefore, the antimicrobial peptide of this invention can be used to prepare products for improving intestinal health and combating diarrhea in animals, such as feed additives or oral liquids.