83 results about "Immune Factors" patented technology

The invention belongs to the technical field of bioengineering, and particularly relates to leuconostoc mesenteroides subsp. Mesenteroides FTCM002 and application thereof in preparation of dendrobium officinale leavening with the effects of reducing blood sugar, nourishing the stomach and resisting inflammation. Dendrobium officinale is subjected to enzymolysis and inoculated with the strain for fermentation, and the obtained fermented dendrobium officinale has the effect of inhibiting alpha-glucosidase; the gastric mucosal injury can be relieved, the expression of VEGF, IL-6, IL-8 and TNF-alpha in the gastric mucosal injury is reduced, and the effect of nourishing the stomach is achieved; the cell phagocytic rate can be increased, the NO concentration and the content of immune factors IL-6 and TNF-alpha can be increased, and the effect of enhancing the immunity is achieved; the expression quantity of IL-6, IL-1beta and TNF-alpha in inflammatory cells can be reduced, and the anti-inflammatory effect is achieved.

The invention relates to the technical field of biomedical engineering, in particular to engineered probiotics with a surface-modified protective shell, a preparation method and application of the engineered probiotics, the engineered probiotics with the surface-modified protective shell comprise the protective shell and engineered probiotics, and the engineered probiotics are coated with the protective shell; the engineered probiotics are subjected to surface modification through immune regulation factors, and metal ions are loaded on the surfaces of the engineered probiotics subjected to surface modification; the engineered probiotics with the surface modified protective shell can regulate immune factors, can be targeted and colonized in colon and promote repair of mucosa surface, and has a better treatment effect on enteritis, so that the engineered probiotics are expected to be applied to preparation of enteritis pharmaceutical preparations; according to the preparation method of the engineering probiotics, raw materials are easy to obtain, the operation process is simple and convenient, reaction conditions are controllable, and large-scale production is facilitated.

The invention discloses a preparation method and application of an ovotransferrin oligomer with an immunomodulatory effect, and a function enhanced nano oligomer is constructed through an enzymatic crosslinking and dynamic hot pressing synergistic treatment technology. The preparation method comprises the following specific steps: preparing an ovotransferrin solution, adjusting the pH value to 1.5-2.0, carrying out high-pressure homogenization and double-frequency ultrasonic treatment after pre-denaturation, adding TG enzyme to catalyze, crosslink and induce controllable aggregation, and finally freeze-drying to obtain oligomer powder, the oligomer is of a nano-scale spherical structure, the particle size is 50-200nm, the solubility is good, and the immunoregulation function is remarkably enhanced. Comprising the steps of promoting dendritic cells to secrete immune factors, prolonging the survival time of nematodes under bacterial infection and improving the immune function of immunosuppressive mice. The method disclosed by the invention is simple in process, green and environment-friendly, suitable for the fields of functional health-care products, pharmaceutical preparations, formula foods with special medical purposes and the like, and wide in application prospect.

The invention discloses a giant salamander immune regulation meat peptide and application thereof. The meat peptide comprises one or more than two polypeptides selected from SEQ ID NOs: 1-5. The polypeptide can enhance cellular immune response, such as enhancing macrophage proliferation activity, improving macrophage nitric oxide secretion level, enhancing macrophage phagocytic ability, enhancing macrophage antigen presentation ability, enhancing immune organ functions, regulating T cell subpopulation, improving cellular immune factor level and enhancing immune globulin level.

The invention belongs to the technical field of radiooncology, artificial intelligence and medical image fusion, and particularly relates to a model construction method and system for predicting the radiation pneumonitis risk of a patient receiving immune combined radiotherapy. According to the method, for a non-small cell lung cancer patient receiving immune checkpoint inhibitor (ICIs) combined radiotherapy, clinical information and radiotherapy dose parameters of the patient are collected, a positioning CT image and dynamic immune factor data are simulated, multi-source features including radiomics features, an immune change curve and dose volume indexes are extracted, and a multi-modal fusion prediction model is constructed. The system integrates automatic data input, feature extraction, model training and risk output modules, can output individual radiation pneumonitis risk scores, provides risk grading and dose intervention suggestions, and has high prediction accuracy and clinical interpretability. The method can be widely applied to individualized risk management and precise treatment of non-small cell lung cancer immune combined radiotherapy patients.

The invention provides a culture method of fibroblast stem cells and application of the fibroblast stem cells in facial beautification, and relates to the technical field of cell biology. The culture medium provided by the invention comprises a basic culture medium, a growth factor, an immunomodulatory factor, an antioxidant protective agent and amino acid, the growth factors comprise bFGF, TGF-beta and EGF (Epidermal Growth Factor); the immunoregulation factors comprise IL-6 (Interleukin-6) and IL-10 (Interleukin-10); the antioxidant protective agent comprises vitamin C and glutathione. Through scientific combination of the growth factor, the immune factor and the antioxidant, the culture medium and the culture method significantly improve the proliferation efficiency, the passage frequency and the functional stability of the fibroblast stem cells, and efficient and safe cell products are obtained.

The invention discloses a biological preparation suitable for young pigeon growth and a preparation method and application thereof, and belongs to the technical field of feeds, the biological preparation comprises the following components by weight: 8-40 parts of a microbial agent, 1-10 parts of an additive, 1-10 parts of an antioxidant, 0.5-5 parts of peptidoglycan, and 40-60 parts of montmorillonite; the microbial agent comprises bifidobacterium animalis, lactobacillus acidophilus, lactobacillus paracasei and lactobacillus reuteri; the additive comprises at least one of yeast beta-glucan and astragalus polysaccharide. According to the biological preparation suitable for young pigeon growth, montmorillonite is used as a base material, under the combined action of the microbial inoculum, the additive, the antioxidant and peptidoglycan, the biological preparation capable of adjusting intestinal health of breeding pigeons and improving the immunity of the breeding pigeons is obtained, meanwhile, immune factors in pigeon milk can be improved, and the growth of young pigeons is promoted. The effects of improving the growth performance of the young pigeons and reducing the elimination rate of the breeding pigeons and the young pigeons can be achieved, so that economic benefits are improved, and wide application prospects are achieved.

Provided is a method of optimizing animal colostrum for human consumption by removing allergenic components and combining one or more immune factors to enable the composition to target the mucosal barriers, thereby enhancing mucosal integrity and increasing immune support. The composition is purified to remove one or more of lactose, casein proteins, fats, and / or oils, thereby creating a product that is devoid of allergenic components. The composition may be combined with a prebiotic, and / or natural sweeteners, additional vitamins, herbs, and / or minerals, and flavoring ingredients. The resulting product forms an instantly dissolvable powder that can be distributed in single serve packets, aerosolized spray, and / or in a chewable form. Furthermore, the composition can be used as an ingredient in food and beverages, in infant formula, in medical foods, or in over-the-counter drugs. Also provided is a method of manufacturing the bioactive composition.

The application provides a construction method of a humanized keloid mouse model, comprising the following steps: BAC plasmid construction and preparation; superovulation of experimental mice and collection of zygotes; pronuclear microinjection of zygotes; post-injection embryo transplantation; genotype identification; breeding and genetic analysis; and verification of construction results of the humanized immune system by immunohistochemistry and immunofluorescence. In the application, peripheral blood mononuclear cells treated by sCD27 and skin around keloids (homologous cells and tissues) are transplanted into NSG-MHC-DKO immune-deficient mice with overexpression of CD70 genes, so that HLA rejection of different homologous immune cells and tissues can be avoided, the internal microenvironment of keloids and the interaction between the internal microenvironment and the immune system can be restored to the maximum extent, and the influence of immune factors on the occurrence and development of keloids can be realized in vitro. The application first discovers and verifies that the activation of the CD27-CD70 axis can promote the occurrence and development of keloids, and provides a suitable animal model for the research and development of anti-keloid drugs, especially immunotherapy.

The invention provides application of M1 protein related substances in preparation of medicines for treating diseases caused by avian influenza viruses. The application is any one of the following applications: application of Y1 in preparation of medicines for preventing and / or treating diseases caused by avian influenza viruses or avian influenza virus infection; the Y2 is applied to preparation of an avian influenza virus inhibitor; the invention discloses application of Y3 in inhibition of avian influenza virus replication. After a mouse is immunized by the M1 recombinant protein, immune factors related to T cells are increased, secretion of proinflammatory factors is reduced, preparation is made for protecting a body from virus attack, and the M1 recombinant protein is also an expression for laterally improving the immunity of a host. The M1 protein has a good immune effect as an immunogen. The invention has application value in treatment of avian influenza virus and similar viruses.

The present application relates to the field of microbial technology, and particularly relates to bifidobacterium BB36 with improved antigen presentation ability and enriched calcium, iron and selenium, probiotics and application thereof. Bifidobacterium bifidum The present application provides bifidobacterium BB36 with activated macrophages, up-regulated immune factor expression level, and activated dendritic cells, enhanced antigen presentation ability in immune process, and enriched calcium, iron and selenium, and can be used as a biological carrier of mineral elements, in addition, can inhibit the growth of escherichia coli and staphylococcus aureus, and protect intestinal health of the body.

The invention provides an application and a method of an RSAD2 agonist glycopyrronium in resisting RSV infection. The method comprises the following steps: S1, virtual screening and molecular docking; s1.1, performing virtual screening and molecular docking; s1.2, carrying out molecular dynamics simulation; s2, cell culture and RSV infection; s3, performing RT-qPCR detection on the expression of the RSAD2 and the virus load of the RSV; s4, performing Westernblot analysis on the influence of a small molecule compound on the expression of the RSAD2; s5, CCK8 is used for detecting the toxicity of the compound to Beaas-2b cells; s6, carrying out indirect immunofluorescence detection on a fluorescence signal of the RSV; s7, animal model establishment; s8, carrying out HE dyeing; s9, carrying out enzyme-linked immunosorbent assay (ELISA); and S10, statistical analysis. According to the present invention, the small molecule agonist targeting the host immune factor RSAD2 is screened, the endogenous antiviral factor is activated from the host perspective, the drug resistance problem caused by direct virus targeting is avoided, the RSAD2 is adopted as the host natural antiviral molecule, the activation strategy has good targeting property and good safety, and the efficient anti-RSV effect under the low toxic-side effect is achieved.

The invention discloses immune factor detection test paper and a preparation method thereof, and relates to the technical field of biomedicine. The test paper structurally comprises a bottom plate, a sample pad, a binding pad, a nitrocellulose membrane and absorbent paper, and multi-index joint detection is realized by coating a specific detection antibody on the binding pad and coating a specific capture substance on a detection line. The test paper has the advantages of simplicity and convenience in operation, rapidness in detection, multi-index joint detection and the like, and can provide powerful support for diagnosis of immune-related adverse reactions and other diseases.

This application belongs to the field of microbial technology, and mainly relates to a strain of *Pediococcus lactis* BPA03 and its applications. *Pediococcus lactis* BPA03 is classified and named *Pediococcus lactis*. Pediococcus acidilactici This *Pediococcus lactis* strain BPA03 was deposited on September 28, 2025, at the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, with accession number CGMCC No. 36109. It inhibits skin melanin deposition and improves UV photoaging, suppresses the gene expression levels of immune factors such as IL-1β, IL-6, IL-8, and TNF-α, improves the activities of superoxide dismutase, catalase, and glutathione peroxidase, inhibits vinorelbine-induced neutropenia, and improves the inhibition of immune factor expression induced by vinorelbine, thus exhibiting comprehensive anti-aging effects.

The invention discloses a duck plague virus LORF2 gene deleted strain as well as a construction method and application thereof, and belongs to the technical field of gene engineering. The construction method comprises the following steps: S1, by taking a pEP-Kan-S plasmid as a template, carrying out PCR (Polymerase Chain Reaction) amplification on a delta LORF2-Kan targeting fragment; s2, electrically transferring the targeting fragment to a competent state to construct a positive bacterium of which the LORF2 gene is replaced by a Kan resistance gene; s3, knockout of Kan resistance genes through homologous recombination; and S4, carrying out virus rescue to obtain the LORF2 gene deleted strain. The duck plague virus LORF2 gene deletion strain constructed by the invention has reduced pathogenicity to ducks and high immune factor level, can completely protect the ducks from being attacked by virulent viruses by immunizing the ducks with an ultra-low dose of 102 TCID50, has a virulent virus removal effect and a neutralizing antibody level which are similar to those of an existing commercial vaccine group, and provides strains, thoughts and technical supports for construction of DPV attenuated live vaccines.

The application discloses a quinazoline-4(3H)-ketone compound and a preparation method, a pharmaceutical composition and application thereof, the structure of the compound is as shown in the formula (I), and the compound comprises isomers, pharmaceutically acceptable salts or mixtures thereof. The compound and the pharmaceutical composition thereof have a high inhibitory effect on PARP7, the inhibitory activity reaches a nanomolar concentration level, can significantly promote the release of immune factors, are used for immunotherapy of tumors, and can exert good drug efficacy at a molecular level and a cell level. In addition, the preparation method of the compound is simple and easy to implement.

This invention belongs to the field of active ingredient extraction and purification technology, specifically a neutral polysaccharide ORP-0 from *Agaricus bisporus* and its applications. The preparation method of this invention includes the following steps: (1) pretreatment; (2) polysaccharide extraction; (3) impurity removal; and (4) separation and purification. This invention obtains a single neutral polysaccharide ORP-0 through defatting pretreatment, ultrasonic-assisted extraction, multiple impurity removal processes, and separation and purification using a DEAE-52 cellulose chromatography column. The method of this invention is simple to operate, has mild reaction conditions, and a complete impurity removal process. The obtained neutral polysaccharide has high purity, with a total sugar content of 92.09%. Furthermore, the obtained neutral polysaccharide ORP-0 enhances the activity of RAW 264.7 macrophages, increases the release of immune factors, enhances the expression of phosphorylated proteins in the MAPK signaling pathway, and activates the body's immune response.

The invention discloses an application of a combination of a ficus pandurata aqueous extract and a PD-1 antibody in preparation of a medicine for preventing or treating colorectal cancer. The ficus pandurata water extract and the PD-1 antibody are creatively combined for use, and experimental results show that on one hand, the combination of the ficus pandurata water extract and the PD-1 antibody can synergistically inhibit tumor growth, improve the anti-tumor immune cell factor level and remarkably enhance the treatment effect on the colorectal cancer CRC; a new direction is provided for breaking through the clinical bottlenecks that the colorectal cancer CRC immunotherapy is low in response rate and limited in single-drug curative effect; on the other hand, the combination of the two drugs has no obvious toxic and side effects on heart, lung, liver and kidney, can reduce adverse effects on the spleen, has a potential protection effect on the important immune organ of the spleen, and is helpful to maintain the normal immune function of the organism.

The invention relates to the technical field of biology, and discloses a kit for predicting clinical pregnancy outcome of first-time frozen embryo transplantation. The kit comprises immune cells and immune factors in blood, the immune cells comprise CD19 < + > B cells, CD3 < + > T cells, CD4 < + > Th cells, CD8 < + > Ts cells, CD4 < + > / CD8 < + > ratio cells, CD56 < + > NK cells, CD68 < + > monocytes, CD86 < + > M1 sample monocytes, D206 < + > M2 sample monocytes and CD163 < + > M2 sample monocytes; the immune factors comprise IL-1beta, IL-2r, IL-6, IL-8 and TNF (Tumor Necrosis Factor)-alpha. According to the application, the importance of the peripheral blood CD163 + M2 sample mononuclear cell on predicting the continuous pregnancy of first embryo implantation of an FE T patient is confirmed, and the peripheral blood CD163 + M2 sample mononuclear cell is prompted to be possibly a biomarker of an FET period.

The invention belongs to the technical field of detection, and particularly discloses a measurement uncertainty evaluation method for measuring immune factors in serum by an ELISA (enzyme-linked immuno sorbent assay) method. According to the method, ELISA detection is carried out on immune factors (such as IL-1beta) in serum of an experimental animal in contact with a medical instrument according to standards such as GB / T16886.20-2015, system analysis comprises nine types of uncertainty sources such as repeated detection, standard substance preparation and sample dilution, the standard uncertainty of each component is calculated through mathematical methods such as rectangular distribution and a least square method, and the standard uncertainty of each component is calculated according to the standard uncertainty. And synthesizing the standard uncertainty and calculating the expansion uncertainty, and finally outputting a standard detection result. The method comprehensively covers the uncertainty source, is rigorous in computational logic and clear in key quality control factors, provides a scientific and complete uncertainty evaluation scheme for medical instrument immunogenicity evaluation and immune factor determination in other experimental scenes, is beneficial to laboratory quality control and detection result optimization, and has wide practicability.

The invention relates to the technical field of deep learning, and discloses a prediction method and system for a liver cancer immunotherapy effect. The method comprises the following steps: carrying out multiple immunofluorescence staining pretreatment on a clinical sample, and collecting forward scattering data, lateral scattering data and multi-channel fluorescence data of mononuclear cells to obtain a cell expression initial data matrix; performing standardization processing to obtain a multi-dimensional feature matrix expressed by the immune factors; performing Bayesian network analysis, calculating a conditional probability relation and a mutual information value between immune cell factors, and constructing an immune factor association network; performing anomaly detection to obtain an immune factor expression anomaly detection result; and performing minimum tail boundary analysis on the immune factor expression anomaly detection result, solving an optimal risk boundary through a semi-definite programming model, and outputting immune factor anomaly quantitative evaluation data, thereby realizing quantitative risk evaluation of the prediction result and refined grading of the prediction result.

The invention discloses a functional food for improving immunity and belongs to the field of health-care foods. Comprising the following substances in parts by weight: 15-20 parts of chitosan oligosaccharide, 4-6 parts of astragalus polysaccharide, 2-4 parts of lucid ganoderma oligosaccharide, 2-4 parts of Chinese wolfberry oligosaccharide, 1-2 parts of codonopsis pilosula polysaccharide, 2-4 parts of ginseng oligosaccharide, 6-8 parts of lentinan, 6-10 parts of Chinese yam polysaccharide, 2-4 parts of oat peptide, 1-2 parts of pericarpium citri reticulatae polysaccharide, 6-8 parts of soybean peptide and 10-15 parts of whey protein peptide. The various components have a synergistic effect, the polysaccharide components can enhance the activity of immune cells, the peptide substances can promote the generation of immune factors, and the synergistic effect of the polysaccharide components and the peptide substances is beneficial for improving the immune defense capability of the body and improving the immunity of the human body.

The invention discloses application of oxidized berberine and neohesperidin in improving intestinal health of broiler chickens, and belongs to the technical field of feed additives. Research finds that oxidized berberine and neohesperidin can increase the intestinal villus height, crypt depth and goblet cell number of broiler chickens and improve intestinal health; the slaughter indexes such as daily weight gain and feed intake can be improved, the feed-weight ratio is reduced, and the slaughter indexes such as carcass weight and semi-eviscerating weight are increased; meanwhile, the serum immune factor and antioxidant index level is improved, the safety is high, and no obvious toxicity exists; and the two components are combined for use, so that a synergistic interaction effect is also achieved. The invention provides a new raw material resource for improving the intestinal health of the broiler chicken and improving the production performance and economic value of the broiler chicken.

The construction method comprises the following steps: amplifying a Cwp8 protein gene on a clostridium difficile genome, constructing the Cwp8 protein gene on a pLEB124 plasmid to obtain a recombinant plasmid pLEB124-Cwp8, and transferring the recombinant plasmid pLEB124-Cwp8 into lactococcus lactis F44 to obtain the clostridium difficile resistant lactococcus lactis, which is named as Lla-Cwp8, and the clostridium difficile resistant lactococcus lactis is named as Lla-Cwp8, and the clostridium difficile resistant lactococcus lactis is named as Lla-Cwp8. The surface of the Lla-Cwp8 disclosed by the invention can express the adhesion protein and realize high yield of Nisin. Experiments prove that Lla-Cwp8 has strong competition, rejection and translocation capabilities on clostridium difficile in vitro, can effectively reduce the survival rate of clostridium difficile and reduce colon shortening in vivo, can significantly reduce the expression of virulence proteins TcdA and TcdB and the expression of immune factors TNF-alpha and IL-6, and has a treatment effect on clostridium difficile infection.

The application belongs to the field of marine biotechnology, and particularly relates to natural polysaccharide-quaternized chitosan nanoparticles with immune stimulation and preparation and application thereof. The nanoparticles formed by natural polysaccharide with negative charge and quaternized chitosan with positive charge are obtained by polyelectrolyte complexation, the particle size is 316.4 nm, and the potential is 31.1 mV; the quaternized chitosan with positive charge and the natural polysaccharide with negative charge are mixed in a mass ratio of 6.25-10:1-4. After immunization of mice, the secretion amount of immunoglobulin IgG and immune factors in the mice is determined, which shows that the nanoparticles have good immune activity, thereby playing an effect in vaccine adjuvant. The application provides a method and guidance for the research on natural polysaccharide and chitosan derivatives as immune adjuvants in recent years.

The application discloses a Bifidobacterium breve capable of relieving psoriasis and an application thereof, and belongs to the technical field of microorganisms. The application screens a Bifidobacterium breve CCFM683, and the Bifidobacterium breve CCFM683 has the effect of relieving psoriasis caused by imiquimod, which is specifically embodied in the following aspects: (1) significantly reducing the weight loss of psoriasis mice; (2) significantly improving the pathological characteristics such as scales, thickening and redness of the skin of psoriasis-like mice; (3) regulating the level of skin immune factors of psoriasis-like mice; and (4) significantly inhibiting the proliferation of keratinocytes of the skin of psoriasis mice. The Bifidobacterium breve CCFM683 has great application prospect in the preparation of food or medicine for preventing and / or treating psoriasis.

A strain of Streptomyces pseudo-light gray XH-1, its microecological preparation and its preparation method. The Streptomyces pseudo-light gray XH-1 of the present invention was deposited in the China Center for Type Culture Collection on August 26, 2024, with the strain deposit number CCTCC NO: M 20241850; the classification name is: Streptomyces pseudo-light gray XH-1, the Latin name Streptomyces pseudogriseolus XH‑1. Its microecological preparation is made by fermenting Streptomyces pseudogriseus XH‑1. The present invention also includes a method for preparing a microecological preparation of Streptomyces pseudogriseus XH‑1. Feeding fish with a fermentation liquid of an appropriate concentration of the strain can significantly improve the immune performance of the fish, especially the specific immune performance. The microecological preparation prepared by the strain also has an antibacterial effect on common fish pathogens such as Aeromonas vernix, Shigella-like Pseudomonas, and Aeromonas anomala. When Streptomyces pseudogriseus XH‑1 is added to the feed to feed fish, it is found that it can significantly increase the expression of specific immune factors and improve the disease resistance of fish.

Disclosed in the present invention are a pyridazinone compound and a preparation method therefor, a pharmaceutical composition thereof, and an application thereof. The pyridazinone compound has a structure as shown in formula (I), and contains an isomer thereof, a pharmaceutically acceptable salt thereof, or a mixture of the isomer and the pharmaceutically acceptable salt. The pyridazinone compound has excellent pharmacokinetic properties in vivo, can also promote the release of immune factors, and in particular, also has excellent antitumor activity in vivo. In addition, the preparation method for the pyridazinone compound is simple and easy.

The present invention discloses an octapeptide with immune-enhancing effect, its preparation and application, belonging to the technical field of biomedicine. The amino acid sequence of the octapeptide is Gly-Phe-Asn-Asp-Leu-Gly-Lys-Arg. This peptide segment can be obtained by artificial synthesis or directional enzymatic hydrolysis of wheat protein. Function verification shows that this octapeptide has the effect of promoting the proliferation of immune cells, mainly manifested in significantly increasing the number of macrophages and neutrophils, and increasing the level of immune factor interferon-γ. The present invention provides a new active substance for enhancing immune function, which can be applied to the preparation of health foods or drugs for preventing and improving immune hypofunction, and has good market prospects and application potential.