14 results about "Virus removal" patented technology

This utility model provides a liquid flow channel structure for a virus removal filter, including an upper cover, several intermediate plates, and a lower cover connected in sequence. Compared with the prior art, the beneficial effects of this utility model are: 1. The venting ports of the pre-filter and post-filter chambers can completely exhaust the gas in the first pre-filter and first post-filter chambers, thereby preventing airlock on the filter membrane due to gas presence and avoiding affecting filtration efficiency; 2. The combination of straight and oblique flow channels, with the oblique flow channel outlet concentrated on one side, facilitates concentrated liquid flow into the second post-filter chamber, and also solves many problems in the filter manufacturing process, improving the utilization rate of the filter membrane; 3. The inlet buffer chamber and outlet buffer chamber allow the filter liquid to be spread smoothly and evenly on the filter membrane, avoiding the impact of liquid inflow on the filter membrane filtration effect.

This invention discloses a method for virus-free tissue culture using terminal buds of *Prunus armeniaca* rootstock, belonging to the field of plant tissue culture technology. The method includes: selecting terminal buds of *Prunus armeniaca* rootstock for pretreatment, sterilizing them with ethanol and mercuric chloride, and then immersing them in a virus-free pretreatment solution containing ribavirin and triazole nucleoside for virus removal; inoculating the virus-free terminal buds into WPM-based differentiation induction medium to obtain clustered buds; culturing the clustered buds in 1 / 2 MS-based proliferation subculture medium to obtain proliferating buds; inoculating the proliferating buds into 1 / 3 MS-based rooting medium to induce rooting; and finally, after hardening off and transplanting into a vermiculite-garden soil-humus substrate for acclimatization. This invention optimizes the virus removal and tissue culture system based on the characteristics of *Prunus armeniaca* rootstock, achieving a virus removal rate ≥95%, a terminal bud differentiation survival rate ≥85%, a rooting rate ≥90%, and a transplant survival rate ≥88%. This enables rapid and large-scale propagation of virus-free *Prunus armeniaca* rootstock seedlings, solving the problem of low propagation efficiency in traditional methods and providing high-quality rootstock support for the *Prunus armeniaca* industry.

The present application relates to the technical field of aquatic plant virus separation, and discloses a method for separating aquatic plant viruses and application thereof, wherein the method comprises mixing amino-functionalized carbon nanomaterials and aquatic bodies containing plant viruses. The amino-functionalized carbon nanomaterials used in the method can be combined with the plant viruses in the aquatic bodies, thereby improving the adsorption capacity of the amino-functionalized carbon nanomaterials for the plant viruses. The method is simple, efficient and low in cost, and does not produce toxic substances, thereby avoiding secondary pollution possibly caused by chemical disinfection, providing an economic and sustainable solution to the problem of plant virus pollution, and showing a broad application prospect in the field of aquatic plant virus removal.

The present invention relates to a method for manufacturing a hydrophilic microporous membrane for virus filtration. Specifically, the hydrophilic microporous membrane is manufactured by performing a hydrophilization treatment without using a crosslinking agent, and the microporous membrane for virus removal thus manufactured has excellent water permeability and protein permeability and exhibits good durability against organic solvents.

This application provides a method for characterizing and / or determining the viral removal capacity of hydrophobic interaction chromatography (HIC), including an experimental design for multivariate analysis of viral removal by HIC. The method provides an understanding of the mechanism of viral removal using HIC by executing a D-Optimal experimental design that includes evaluations of multiple factors, such as pH, buffer concentration, column loading concentration, column flow rate, and hydrophobic strength of the HIC column.

The present invention relates to a method for manufacturing a hydrophilic microporous membrane for virus filtration. Specifically, the hydrophilic microporous membrane is manufactured by performing a hydrophilic treatment without using a crosslinking agent, and the microporous membrane for virus removal thus manufactured has excellent water permeability and protein permeability and exhibits good durability against organic solvents (alcohols).

The present disclosure describes a unique viral peptide (VP) vaccine for preventing or treating viral diseases. The vaccine is produced synthetically and includes no production steps in biological cells (e.g. E. coli, CHO cells, yeast cells) that would require subsequent endotoxin assays / removal or viral clearance procedures. The hC peptide is synthesized separately from the VP, and following self-assembly of the hC, the VP is covalently coupled to form the VP-hC conjugate which can serve as a vaccine for preventing or treating viral diseases. The hC includes heptad repeats following a specific pattern. Optionally, the VP-hC conjugate further includes one or more T-cell epitopes at the N- and / or C-terminus of the one or more amphipathic alpha-helices. The present disclosure also describes compositions comprising immunogenic compositions including VP-hC conjugate.

The application discloses a filtering device for virus removal filtration of a protein-containing feed liquid and a method for virus removal filtration of a protein-containing feed liquid, and relates to the technical field of biological filtration. The filtering device comprises a filtering unit, a packaging layer, a liquid inlet channel, a filtrate channel and a filtering layer, wherein the filtering layer comprises a virus removal membrane, the LRV of the virus removal membrane for virus impurities is not less than 4, and the protein yield is not less than 98%, the virus removal membrane comprises a pre-filtering layer and a separation layer for intercepting viruses and having a pore size smaller than that of the pre-filtering layer, and at least one pre-filtering layer is arranged on the surface of the side of the virus removal membrane away from the filtrate guide screen. The filtering device has the advantages of simple structure, convenient operation, good virus removal effect of the virus removal membrane on the protein-containing feed liquid, and the like.

The application discloses a kind of long-term treatment strategy making methods for cervical cancer prevention and treatment of HPV, belong to medical information processing technical field.The method includes: obtaining the digital HPV health record containing multiple HPV typing detection results, cervical cytology history record and individual physiological characteristic parameters;Three-dimensional treatment situation cube is constructed by virus infection state subspace, cervical tissue state subspace and treatment intervention state subspace;In the cube, state backtracking and forward-looking deduction algorithm based on time axis are used, virus clearance probability field and lesion outcome probability field are calculated;High-risk persistent infection space-time region and low-risk self-healing tendency space-time region are demarcated;Current patient state is projected to the cube, if fall into high-risk region, then trigger long-term treatment strategy generation process including multiple treatment stages, output intervention opportunity, intensity and state transition condition of each stage.

The utility model discloses a kind of nanometer membrane virus removal small test device, belong to biological product and blood product production technical field.The device includes filter tank, nanometer membrane and filtrate collection weighing component, the top of the filter tank is connected with air inlet pipe, air pressure detection assembly and air pressure regulating assembly are equipped on the air inlet pipe, and air pressure detection assembly is located between air pressure regulating assembly and filter tank, the liquid inlet of the nanometer membrane is connected with the bottom of filter tank by pipeline, and hydraulic detection assembly A is equipped on the pipeline, the liquid outlet of the nanometer membrane is connected with drain pipe, and hydraulic detection assembly B is equipped on the drain pipe, the filtrate collection weighing component is placed just below the liquid outlet of drain pipe.The real-time data of power gas source pressure value, feed liquid nanofiltration before and after pressure value, reflect the nanofiltration pressure real-time change situation of nanometer membrane;The real-time data record of feed liquid weight after nanofiltration, the change trend of nanofiltration load and nanofiltration effect of nanometer membrane is evaluated by calculation.

The present application relates to the field of fruit tree cultivation, and specifically discloses a kind of Huanniu apple rootstock rapid and efficient propagation method, comprising: explant preparation: with Huanniu apple seedling stem tip as material, 0.05% nano silver+40kHz ultrasonic disinfection (contamination rate≤2.5%), inoculated in QL+1.0mg / L TDZ+35g / L sorbitol induction medium;Light and temperature synergistic proliferation: under blue light: red light=4:6, day and night temperature difference 8℃ (day 26℃ / night 18℃), using MS+0.5mg / L mT+0.5g / L activated carbon culture medium, realize proliferation coefficient 5.8 times;Anti-lodging rooting: add 1.0mg / L IBA and 0.3mg / L PP333 in 1 / 2QL culture medium, rooting rate≥94.2%, root system roughness improves 40%;Gradient seedling: through closed bottle (100% humidity), open seam (85%), full open (65%) three stages of hardening-off, transplant to apple branch debris: perlite=3:1 matrix, survival rate≥91.5%.Effect: virus removal rate≥98.3% (verified by SGV / ACLSV specific primer);Propagation cycle 45 days, compared with traditional method, shorten 75%;Single plant cost 0.38 yuan, after grafting, orchard yield per mu increases 20%.