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218 results about "Virus removal" patented technology

Virus removal and rapid propagation technology of sweet potato variety 'Shangshu 19' and culture material

InactiveCN101611697AImprove immunitySolve the serious sweet potato virus diseaseCultivating equipmentsPlant tissue cultureDiseaseGermplasm
The invention is a virus removal and rapid propagation technology of sweet potato variety 'Shangshu 19', comprising the following steps: selecting plants which are robust without diseases and insect pests and have characteristics of the variety from growing plants; clipping stem with stem tip; removing visible leaves; sterilizing; peeling the stem tip with 1-2 leaf primodia via a dissecting needle; quickly inoculating into a differentiation and induction culture medium; trimming the sterilized seedling into stem segments with 1-2 internode(s) and switching into an enrichment medium after virus detection; selecting sterilized seedling in vigorous growth to cut into small segments with 1-2 internode(s) and switch into a rooting medium; carrying out domestication on seedling after rooting. The invention has stem tip in virus removal and stem segment in rapid propagation, solves the problems of severe virus disease for sweet potato, poor quality of new cultured breed, germplasm degradation, low yield and the like propagated by using sweet potato block and stem. The invention can artificially control the growing conditions, can not be influenced by nature, and has the advantages of small amount of material use, economical culturing materials, short growth cycle, large propagation coefficient and convenient management and is favor of industrial production.
Owner:周玉玲

Method for removing PVS viruses of potato test-tube plantlets

The invention provides a method for removing PVS viruses of potato test-tube plantlets. The method comprises the steps of stripping the stem tips of the pretreated test-tube plantlets and then putting the test-tube plantlets into a stem tip culture medium with the stem tip facing upwards and the section facing downwards for culture, next, culturing the stem tips for 40-50 days under the conditions of a temperature within the range of 22+/-2 DEG C, the illumination time of 12h-15h/d and the illumination intensity of 2000-3000lx, in the period, transferring the stem tips above 0.5cm long to an MS culture medium, and continuously culturing the undifferentiated stem tip callus or small plantlets less than 0.5cm long, and finally, detecting the stem tip seedlings by use of enzyme linked immunosorbent assay, and remaining the satisfied stem tip seedlings, thereby obtaining the potato seedlings without PVS through the whole period of 120-180 days. The method is high in PVS virus removal rate, suitable for batch operations of removing the PVS viruses of the potato variety resources, and capable of obtaining the satisfied seedlings at a time and avoiding repeated labor; besides, the method is high in virus removal rate and stable in virus removal effect, and the detection cost can be reduced.
Owner:云南省农业科学院经济作物研究所

Method for preparing human thrombin from cold-removing glue plasma

The invention discloses a method for preparing human thrombin from cold-removing glue plasma. Firstly, anion resins are used to absorb prothrombin in the cold-removing glue plasma, then elution and filtering are conducted, and a prothrombin solution is obtained; secondly, the prothrombin solution is activated through a CaCL2 solution, and a thrombin solution is obtained; thirdly, S/D viral inactivation is conducted on the thrombin solution; fourthly, chromatography is conducted through cation columns, and a purified thrombin solution is obtained; fifthly, ultrafiltration, dialysis and concentrate are conducted on the thrombin solution, and the thrombin solution with the titer meeting the product specification requirement is obtained; sixthly, a filter element of 20 nm in size is used to conduct virus removal filtering; seventhly, sterilization filtering is conducted through a filter element of 0.22 microns in size, and then subpackage is conducted according to the required specifications; eighthly, freeze-drying is conducted, and dry heat viral inactivation is conducted on freeze-drying powder, and a human thrombin product is obtained. According to the method, the thrombin is purified through the one-step cation columns, operation is simple, thrombin specific activity is high, and safety of clinic use of the product is guaranteed through a three-step virus elimination mode.
Owner:上海洲跃生物科技有限公司

Outdoor transplanting and temporary planting method of stock seedlings of healthy sugarcane seedlings

InactiveCN102577785AImprove tillering rateStrong tillering rateHorticultureAxillary budSucrose
The invention relates to an outdoor transplanting and temporary planting method of stock seedlings of healthy sugarcane seedlings, comprising the following processes: outdoor land preparation and bed preparation; transplantation matrix preparation; sparse planting and transplanting of the stock seedlings; short-term heat preservation and moisture preservation; management of water, fertilizers andpesticides; and outplanting. In the invention, the production technology system of the biochemical virus removal of healthy sugarcane seedlings is established, the technical problems of virus removaland rapid propagation of sugarcane axillary buds are effectively solved; the seedling-growing cost is low; the seedling-growing time is short; the seedling-growing survival rate is high; the obtainedbiochemical virus-free healthy sugarcane seedlings have the characteristics of strong tillering rate, high stem forming rate, rapid growth speed, high ratooning rate and the like; the yield per unit area is improved by more than 30%; the sucrose content is improved by 0.5-1.0%; and the using amount of seedlings is saved by more than 60%. The outdoor transplanting and temporary planting method of the invention has great significance for solving the problems of serious degeneration of the sugarcane varieties and low breeding speed of the certified seedlings, plays an active role of promoting the popularization and application of healthy sugarcane seedlings and has important economic and social benefits.
Owner:INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI

Human coagulation factor VIII preparation method

The invention discloses a human coagulation factor VIII preparation method. In the preparation process of a human coagulation factor VIII, a two-step filter press technique is adopted during treatment of human plasma initial materials, that is, a K700 filter plate is adopted for filter pressing after cryoprecipitate dissolution, the filter press liquor is collected, the pH value is adjusted, a 2% aluminium hydroxide gel is added for adsorption, and then an EK filter plate is adopted for filter pressing. The two-step filter press technique is adopted to improve the separation effect, meanwhile, the aluminium hydroxide gel adsorption method is used together to remove a coagulation factor on which vitamin K depends, the aluminium hydroxide gel does not adsorb the human coagulation factor VIII, and the product yield is high. In the preparation process, the two-step filter press technique replaces a traditional two-step high-speed centrifuging method, a CUNO DELP deep filter element is adopted for filtration, a two-step gradient dialysis method is adopted, a re-dissolution and re-freezing process is added to a freeze-drying process, and meanwhile, various virus removal/inactivation technologies are adopted in the production process, so that the product yield can be improved, the risk of virus spreading can be reduced, and the clinical medication security is improved.
Owner:华润博雅生物制药集团股份有限公司
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