107 results about "Virus removal" patented technology

To provide a method of using a virus removal device that enables the rapid elimination of viruses contained in the air to be treated and viruses contained in the liquid by selecting the liquid to be sprayed from a liquid spray mechanism.SOLUTION: As the liquid to be sprayed from a liquid spray mechanism 3 in a virus removal device, used is an aqueous solution containing an ammonium salt as a radical generation catalyst and a halous acid salt as a radical generation source.SELECTED DRAWING: Figure 1

The invention provides a nylon 6 nanofiber virus-removing filtering membrane and a preparation method and application thereof.The nylon 6 nanofiber virus-removing filtering membrane comprises a base material and a nylon 6 nanofiber membrane compounded to the surface of the base material, the average pore size of the virus-removing filtering membrane is 20 nm, the virus-removing filtering membrane plays a role in blocking parvoviruses, the surface of the virus-removing filtering membrane is charged, and the surface of the virus-removing filtering membrane is not prone to being damaged. Proteins with the same charges on the surface can be prevented from adhering to the surface of the fiber. In the preparation process of the virus-removing filtering membrane, the nylon 6 nanofibers with a certain diameter range are selected and compounded on the surface of the base material in a stable dispersion state, the uniform microporous virus-removing filtering membrane is prepared, the effect that parvoviruses are blocked and protein molecules are not easily intercepted is achieved, the hydrophilicity of the nylon 6 nanofibers is relatively good, and the virus-removing filtering membrane has a good application prospect. The non-specific adsorption of the filtering membrane on the protein is reduced; the nylon 6 nanofiber virus-removing filtering membrane can be applied to the field of biological medicine products, and has important significance on separation and purification of biological preparations.

The invention belongs to the technical field of protein purification, and particularly relates to a recombinant human metapneumovirus pre-fusion F protein purification method which comprises the following steps: filtering fermentation liquor by using a deep filter to obtain fermentation clear liquor; capturing the recombinant hMPV F protein from the fermentation clarified liquid by using hydroxyapatite to obtain a purified liquid 1; carrying out virus inactivation on the purified solution 1 by adopting a low-pH method to obtain an inactivated solution; carrying out anion exchange chromatography on the inactivated solution to obtain a purified solution 2; carrying out composite weak anion chromatography on the purified solution 2 to obtain a purified solution 3; performing UF / DF on the purified liquid 3 to obtain an ultrafiltrate; and carrying out virus removal nanofiltration and sterile filtration on the ultrafiltrate to obtain a recombinant hMPV F stock solution. The method is simple and convenient, has good repeatability, is suitable for large-scale production, and can be effectively used for producing the recombinant human metapneumovirus pre-fusion F protein vaccine.

The invention discloses a method for extracting human placenta tissue and preparing human placenta tissue injection, which comprises the following steps: screening placenta, unfreezing, soaking, rinsing, draining, weighing, preparing liquid, mincing, grinding and hydrolyzing, adding acid water to adjust the pH value for acid hydrolysis, adding alkaline water to adjust the pH value for alkaline hydrolysis after hydrolysis, and then inactivating viruses. The preparation method comprises the following steps: preparing a human placenta tissue solution, carrying out virus inactivation, carrying out rough filtration and fine ultrafiltration to remove bacteria and filtration, carrying out nanofiltration virus removal treatment by using a nano-membrane to obtain a sterile human placenta tissue solution stock solution, carrying out dilution preparation on the human placenta tissue solution stock solution according to the stock solution concentration, carrying out sterile subpackaging in bottles, sealing and detecting. The prepared human placenta tissue fluid is high in biological activity, the biological activity of the human placenta tissue fluid is effectively guaranteed, and meanwhile the biological safety of the human placenta tissue fluid is guaranteed by adopting a two-time virus inactivation and removal method.

The invention provides a novel three-antigen HSV-2 adjuvant subunit vaccine as well as a preparation method and application thereof. The vaccine comprises herpes simplex virus recombinant protein and a composite adjuvant, the herpes simplex virus recombinant protein comprises extracellular domains of three proteins, namely HSV-2gE, gD and gC, wherein the amino acid sequences of the extracellular domains of the HSV-2gE, the gD and the gC are respectively shown as SEQ ID NO.1, SEQ ID NO.2 and SEQ ID NO.3; and the composite adjuvant is composed of CpG oligodeoxynucleotide and QS-21. Experiments prove that by adding the composite adjuvant composed of CpG oligodeoxynucleotide and QS-21, the immunogenicity, in-vivo protectiveness, virus removal and other immune functions of the HSV-2 subunit vaccine can be remarkably improved; meanwhile, the vaccine is a cross immune protection vaccine of an HSV-2 virus strain and an HSV-1 virus strain.

The invention relates to a verification device and a verification method for verifying filterability of a virus removal filter. The verification device comprises a sample introduction part, a pre-filter, a mixing part, a to-be-verified virus removal filter and a collection part which are sequentially connected in series, the verification device further comprises a virus adding part and a sampling part, the virus adding part is communicated with an inlet of the mixing part and used for adding viruses into the mixing part, and the sampling part is communicated with an outlet of the pre-filter. The sampling part is communicated with the outlet of the mixing part and is used for sampling the mixed liquid flowing out of the mixing part; the sampling component is further provided with a pressure balancing component used for balancing the pressure difference between the mixing component and the sampling component when sampling is started. According to the verification device and the verification method, the problem of re-aggregation in the verification process can be avoided, so that more real and credible filterable data support is provided for application of the virus removal filter.

The invention relates to the field of microorganisms, and particularly discloses a highly pathogenic gosling plague virus and a preparation method of an egg yolk antibody of the highly pathogenic gosling plague virus. The invention relates to a gosling plague virus, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), is named as the gosling plague virus RD2311B-E21, is preserved on April 22, 2024, and has the preservation number of CGMCC NO.45964. The gosling plague virus is named as the gosling plague virus RD2311B-E21. The preparation method of the egg yolk antibody comprises the following steps: proliferating and inactivating gosling plague virus RD2311B-E21 to prepare an immunogen; respectively injecting the immunogen into the laying hens for four times to obtain four-immunized eggs; the preparation method comprises the following steps: soaking a tetra-immunized egg in a bromogeramine solution, performing spray fumigation with glutaraldehyde, then separating yolk, treating the yolk with disodium hydrogen phosphate to obtain a turbid solution, and performing acidification extraction, degreasing with octanoic acid, degerming filtration, virus removal by ultrafiltration, ultrafiltration concentration and subpackaging to obtain a finished product. The compound has the advantages of high purity, high titer and good treatment, prevention and control effects.

Disclosed are antibodies to anti-hepatitis B surface antigen (HBsAg) (especially humanized antibodies), nucleic acid molecules encoding same, methods for preparing same, and pharmaceutical compositions containing same. The antibodies have higher affinity for HBsAg at neutral pH than at acidic pH, thereby significantly enhancing the virus clearance efficiency and prolonging the virus inhibition time. The antibodies and the pharmaceutical compositions can be used for preventing and / or treating HBV infections or diseases related to HBV infections (e.g., hepatitis B), for neutralizing the virulence of HBV in a subject (e.g., a human), for reducing the serum level of HBV DNA and / or HBsAg in the body of the subject, or for activating the humoral immune response of the subject (e.g., a chronic HBV infected or chronic hepatitis B patient) against HBV.

The invention belongs to the technical field of sweet potato seedling detoxification, and particularly relates to a sweet potato seedling detoxification rapid propagation method and application, and the sweet potato seedling detoxification rapid propagation method comprises the following steps: carrying out heat treatment culture on sweet potato seedlings at 36-38 DEG C, disinfecting, and taking stem segments with the length of 0.5-1.5 cm as explants; inoculating the explant into a primary culture medium for culturing; after multiple shoots are induced from the explants, inoculating the explants into a subculture medium for culturing; cutting the cultured cluster buds into single buds, inoculating the single buds into a rooting culture medium for culturing, and detecting the detoxification effect after bud seedlings root to obtain detoxified sweet potato tissue culture seedlings. By means of the method, virus detoxification of the sweet potato seedlings can be achieved, the detoxification rate reaches up to 95%, the survival rate reaches 96.7%, the seedling breeding period of 2-3 months is shortened to 45-50 days, the health level and breeding efficiency of the seedlings are improved, and the yield and quality of sweet potatoes are further improved.

The application provides a virus-removing polyether sulfone porous membrane and a preparation method thereof. The virus-removing polyether sulfone porous membrane comprises, in sequence along a thickness direction, an outer layer region and an inner layer region, wherein the average pore size of the outer layer region is 10-20 nm, the thickness of the outer layer region is 20-50 microns, the average pore size of the inner layer region is 5-10 microns, and the thickness of the inner layer region is 60-150 microns; and the virus-removing polyether sulfone porous membrane is prepared by a method comprising at least the following process: taking polyether sulfone as a raw material, and obtaining the virus-removing polyether sulfone porous membrane under the action of a water-soluble diluent and a hydrophilic additive; wherein the water-soluble diluent comprises ethylene carbonate and polyethylene glycol. The virus-removing polyether sulfone porous membrane has a high virus removal rate and a high protein permeation rate.

The application belongs to the technical field of woody plant asexual propagation, and particularly relates to a method for establishing a tissue culture and rapid propagation system of Eucommia ulmoides stem segments. The method comprises the following steps: disinfecting Eucommia ulmoides seeds, obtaining sterile Eucommia ulmoides tissue culture seedlings through seed germination induction, cutting stems of the sterile Eucommia ulmoides tissue culture seedlings into stem segments with a length of 0.5 cm to 1 cm, inoculating the stem segments into an adventitious bud induction medium to perform adventitious bud induction culture, forming clustered adventitious buds after 20 days of the adventitious bud induction culture, separating the clustered adventitious buds into multiple individual adventitious buds, and transferring the individual adventitious buds into a rooting medium to perform rooting and seedling strengthening culture, forming a large number of adventitious roots after 10 days of the rooting and seedling strengthening culture, and obtaining complete regenerated Eucommia ulmoides plants. The method for establishing the tissue culture and rapid propagation system of Eucommia ulmoides stem segments has the advantages of short rapid propagation cycle, high rapid propagation efficiency, no seasonality limitation, good excellent traits maintenance, small space occupation, and virus removal.

Fluorescent viral particles, including viral particles and a fluorescent dye conjugated to the viral particles, are used for viral contaminant removal testing as part of viral clearance evaluation for biological medical product preparation processes. The conjugated fluorescent dye adds only marginally to viral particle size, but provides versatility both for rapid screening of alternative viral removal approaches during process development and for final process validation, as the fluorescent viral particles have fluorescent activity throughout the viral clearance evaluation process, permitting rapid and consistent quantification of initial test solutions, resulting purified solutions, and intermediate solutions of interest at any point during processing under evaluation.

An ultrafiltration membrane for separating viruses and proteins and a preparation method thereof, belonging to the technical field of virus removal flat membranes and virus removal filters. A casting liquid L for forming a sponge-like macroporous layer and a casting liquid S for forming a sponge-like microporous layer are prepared respectively, the casting liquid L is applied to a mobile carrier to form a sponge-like macroporous layer, a non-woven fabric layer is tightly attached to the liquid film of the sponge-like macroporous layer before the sponge-like macroporous layer is solidified, and then the casting liquid S is applied to the non-woven fabric layer to form a sponge-like microporous layer, and the carrier and the double-layer liquid film formed by the casting liquid L and the casting liquid S and the tightly attached non-woven fabric layer are immersed in a coagulation bath after passing through an air section for phase separation and forming, and then washed and dried to obtain an ultrafiltration membrane. The ultrafiltration membrane provided by the present invention has both high flux and high separation accuracy, and meets the actual needs of removing viruses from protein solutions.

A virus removal filtration device, comprising an air inlet device, a pressure reducing device, a pressure control module, a liquid storage device, a virus removal filter column and a collection device. A pressure reducing device and a pressure control module are connected to the pipeline between the air inlet device and the liquid storage device, and electromagnetic solenoid valves are respectively arranged on the pipelines of each liquid storage device; The control system of the virus removal filtration device includes a central processor and a display. The signal acquisition ends of the central processor are respectively connected to the signal output ends of a pressure sensor, a temperature sensor, a pressure sensor before the virus removal filter column and a weighing sensor, for collecting and processing the acquisition signals of each sensor; The signal output ends of the central processor are respectively connected to the signal input ends of the actuators of an automatic regulating valve and an electromagnetic solenoid valve. The device and system of the present invention can accurately control and regulate the pressure, and perform online and timely control on the virus removal filtration device.

A single-use valve arrangement including a valve housing, a diaphragm carried by the valve housing, a pressure sensor, and a controller coupled to the pressure sensor. The valve housing defines an inlet and an outlet, the inlet adapted to be fluidly connected to the outlet of the virus removal filter. The diaphragm divides the valve housing into a first chamber and a second chamber fluidly isolated from the first chamber. The pressure sensor is configured to measure a pressure change in the second chamber due to movement of the diaphragm in response to a pressure change in the first chamber. The controller is configured to determine an actual leak rate of the filter based on the measured pressure change, and the controller is further configured to determine the integrity of the filter by comparing the actual leak rate to an estimated leak rate of the filter.

This utility model provides a liquid flow channel structure for a virus removal filter, including an upper cover, several intermediate plates, and a lower cover connected in sequence. Compared with the prior art, the beneficial effects of this utility model are: 1. The venting ports of the pre-filter and post-filter chambers can completely exhaust the gas in the first pre-filter and first post-filter chambers, thereby preventing airlock on the filter membrane due to gas presence and avoiding affecting filtration efficiency; 2. The combination of straight and oblique flow channels, with the oblique flow channel outlet concentrated on one side, facilitates concentrated liquid flow into the second post-filter chamber, and also solves many problems in the filter manufacturing process, improving the utilization rate of the filter membrane; 3. The inlet buffer chamber and outlet buffer chamber allow the filter liquid to be spread smoothly and evenly on the filter membrane, avoiding the impact of liquid inflow on the filter membrane filtration effect.

The present invention relates to a polymer separation membrane for virus removal capable of protein permeability and a method for manufacturing the same. More specifically, it relates to a polymer separation membrane for virus removal manufactured by dip-coating DPVDF onto a polyethersulfone polymer substrate and then introducing branched polyethyleneimine and amphoteric ions. The present invention can provide a method for manufacturing a separation membrane capable of selectively permeating proteins while maximizing virus removal efficiency by optimizing the type of polymer substrate and the concentration of DPVDF.

The invention relates to the technical field of membrane separation, in particular to a preparation method of a steam-sterilization-resistant hollow fiber membrane component, which is used for preparing a hollow fiber membrane capable of resisting moist heat steam sterilization at 121 DEG C, and the membrane can resist moist heat sterilization under the pressure of 2bar. The preparation method comprises the following steps: bundling the hollow fiber membrane filaments and loading the hollow fiber membrane filaments into a membrane shell, selecting a steam-resistant sterilization type hollow fiber membrane as the hollow fiber membrane filaments, selecting a polysulfone material as the membrane shell material, and then preparing the hollow fiber membrane module by using epoxy resin pouring sealant through centrifugal casting, and the module can be applied to the field of medicine. According to the preparation method of the steam-sterilization-resistant hollow fiber membrane module, the epoxy resin pouring sealant is subjected to the steps of centrifugal pouring, hollow fiber membrane module assembling treatment and hollow fiber membrane module post-treatment, so that high-temperature steam sterilization treatment can be carried out in an environment of 121 DEG C, and the requirements of cell perfusion culture and virus removal are met; therefore, the pharmaceutical process is more effectively applied.

The invention belongs to the technical field of membrane materials, and particularly relates to a preparation method of a dumbbell-shaped polyethersulfone virus-removing membrane, which comprises the following steps: S1, mixing a membrane-forming polymer, an amphiphilic polymer, a non-solvent pore-foaming agent and an organic solvent, stirring, dissolving and defoaming to obtain a uniform and stable membrane casting solution; s2, blade-coating the membrane casting liquid on a support body to form a liquid membrane; s3, exposing the liquid film in humidity-controllable air for a period of time, and carrying out a steam-induced phase separation process; s4, immersing the cast film obtained in the step S3 into a coagulating bath to complete phase inversion so as to form a solid-state film; s5, the formed solid membrane is sequentially subjected to water washing, hole preserving treatment and drying, and the dumbbell-shaped polyethersulfone virus-removing membrane is obtained. The compact layer in the dumbbell-shaped structure ensures the high virus rejection rate, and the porous structure with the open interior reduces the transmission resistance of water to the minimum, so that the unification of the high virus rejection rate and the high water flux is realized, and the protein transmittance is ensured to be more than 98%.

This invention discloses a method for virus-free tissue culture using terminal buds of *Prunus armeniaca* rootstock, belonging to the field of plant tissue culture technology. The method includes: selecting terminal buds of *Prunus armeniaca* rootstock for pretreatment, sterilizing them with ethanol and mercuric chloride, and then immersing them in a virus-free pretreatment solution containing ribavirin and triazole nucleoside for virus removal; inoculating the virus-free terminal buds into WPM-based differentiation induction medium to obtain clustered buds; culturing the clustered buds in 1 / 2 MS-based proliferation subculture medium to obtain proliferating buds; inoculating the proliferating buds into 1 / 3 MS-based rooting medium to induce rooting; and finally, after hardening off and transplanting into a vermiculite-garden soil-humus substrate for acclimatization. This invention optimizes the virus removal and tissue culture system based on the characteristics of *Prunus armeniaca* rootstock, achieving a virus removal rate ≥95%, a terminal bud differentiation survival rate ≥85%, a rooting rate ≥90%, and a transplant survival rate ≥88%. This enables rapid and large-scale propagation of virus-free *Prunus armeniaca* rootstock seedlings, solving the problem of low propagation efficiency in traditional methods and providing high-quality rootstock support for the *Prunus armeniaca* industry.

The invention belongs to the technical field of biology, and particularly relates to a linear tandem antigen epitope polypeptide of African swine fever virus RAN polymerase and application of the linear tandem antigen epitope polypeptide. The amino acid sequence of the linear tandem antigen epitope polypeptide is shown as SEQ ID NO.4 or SEQ ID NO.5; the linear tandem antigen epitope polypeptide is obtained by linearly connecting an epitope polypeptide NP1249L-1, an epitope polypeptide H359L-1 and an epitope polypeptide H359L-2 in series, the epitope polypeptide NP1249L-1, the epitope polypeptide H359L-1 and the epitope polypeptide H359L-2 are obtained through screening, and the linear tandem antigen epitope polypeptide has the functions of inducing ASFV specific T cells and assisting in controlling ASFV infection and removing viruses, and experimental results show that the linear tandem antigen epitope polypeptide has the advantages that the linear tandem antigen epitope polypeptide is a novel linear tandem antigen epitope polypeptide; the epitope polypeptide has the capability of inducing ASFV specific T cell response, and a theoretical basis is provided for subsequent development of polypeptide vaccines and diagnostic preparations based on ASFV protein source epitopes.

The utility model discloses a hollow fiber virus removal filter assembly with a very small cavity volume before filtration. The assembly comprises a liquid inlet joint (1), a hollow pipe (2), an outlet joint (3) and hollow fiber membrane filaments (4). The two ends of the hollow pipe (2) are connected with the liquid inlet connector (1) and the outlet connector (3) to form a closed cavity. And one end of the hollow fiber membrane filament (4) in the hollow tube (2) is hermetically connected with the inner connector (5) through glue and is opened on the end surface, and the other end is self-sealed. The volume of the cavity can be smaller than 0.1 ml before filtration, no residue exists, and feed liquid is saved; only two connectors are arranged, the hollow fiber membrane filaments are only sealed with the liquid inlet, production is simple, and operation is convenient.

The invention discloses a duck plague virus LORF2 gene deleted strain as well as a construction method and application thereof, and belongs to the technical field of gene engineering. The construction method comprises the following steps: S1, by taking a pEP-Kan-S plasmid as a template, carrying out PCR (Polymerase Chain Reaction) amplification on a delta LORF2-Kan targeting fragment; s2, electrically transferring the targeting fragment to a competent state to construct a positive bacterium of which the LORF2 gene is replaced by a Kan resistance gene; s3, knockout of Kan resistance genes through homologous recombination; and S4, carrying out virus rescue to obtain the LORF2 gene deleted strain. The duck plague virus LORF2 gene deletion strain constructed by the invention has reduced pathogenicity to ducks and high immune factor level, can completely protect the ducks from being attacked by virulent viruses by immunizing the ducks with an ultra-low dose of 102 TCID50, has a virulent virus removal effect and a neutralizing antibody level which are similar to those of an existing commercial vaccine group, and provides strains, thoughts and technical supports for construction of DPV attenuated live vaccines.

The invention discloses skin mucosa maintenance liquid capable of killing fungi and HPV (human papillomavirus) as well as a preparation method and application of the skin mucosa maintenance liquid, and belongs to the technical field of sterilization and virus removal. The skin mucosa maintenance liquid is prepared from octanol ether, benzalkonium bromide, phenoxyethanol, yermate, parachlorometaxylenol, taetinic acid, chitosan, keratinase, fluconazole, glycerol, borneol, menthol, absolute ethyl alcohol and tween-40. Wherein the skin mucosa maintenance liquid has an effect of regulating apoptosis induction on abnormal cells and an effect on vaginal flora, and has an obvious inhibition effect on cervicitis and colpitis mycotica, namely an anti-inflammatory effect; meanwhile, apoptosis of cells containing HPV16 and HPV18 genes can be induced, shedding of abnormal cells is promoted, diseased cells are killed, and vaginal tissues with pathological changes are removed.

The invention belongs to the technical field of biology, and particularly relates to a T cell epitope polypeptide based on ASFV F1055L or P1192R protein, the T cell epitope polypeptide comprises a polypeptide F1055L-1, a polypeptide F1055L-2, a polypeptide F1055L-3 and a polypeptide P1192R-1, and the amino acid sequence is shown as SEQ ID NO.1-4. The polypeptide is obtained through screening and has the characteristics of inducing ASFV specific T cells and assisting in controlling ASFV infection and virus clearance. In-vitro experiments prove that the epitope polypeptide has the capability of inducing ASFV specific T cell response, and a theoretical basis is provided for subsequent development of polypeptide vaccines and diagnostic preparations based on ASFV protein source epitopes.

A chromatography device having a housing having an inlet and an outlet. At least two layers of media disposed between the inlet and the outlet inside of the housing forming a media stack, with at least one of the layers comprising a functionalized layer. An optional spacer ring disposed between the two layers of media forming an air gap between them. A non-functionalized sealing layer disposed between the inlet and the outlet inside of the housing as the last layer of media in the media stack within the housing as a fluid passes from the inlet to the outlet through the media stack. A margin of the sealing layer in contact with the housing; the margin being compressed by the housing forming a compressive seal to prevent fluid from leaking to the outlet past the compressive seal.

Provided in the present application are a virus removal filter membrane and a preparation method therefor, belonging to the technical field of membrane separation. The filter membrane preparation method comprises blending a hydrophilic polymer and a bulk polymer to prepare casting solutions having different viscosities, and on the basis of a double-layer homogeneous compounding method and a phase conversion method, preparing the filter membrane. The filter membrane has an integrated structure, and comprises a dense separation layer distributed along the thickness direction of the filter membrane and a pre-filter support layer having a loose sponge structure. The separation layer is adjacent to the support layer, but there is no obvious interface or stratification. The pore diameters of the separation layer and the support layer has a continuous gradient change along the thickness direction, wherein the pore diameter of the support layer is greater than the pore diameter of the separation layer.

The chromatography device has a housing with an inlet and an outlet. At least two media layers are disposed within the housing between the inlet and outlet, at least one of the layers having a functionalized layer. An optional spacer ring disposed between the two media layers forms an air gap therebetween. A non-functionalized sealing layer is disposed within the housing between the inlet and outlet as the last layer of media in the media stack within the housing as fluid passes from the inlet to the outlet through the media stack. A margin of the sealing layer contacting the housing is compressed by the housing to form a compression seal to prevent fluid from leaking past the compression seal to the outlet.

The invention relates to the technical field of water plant virus separation, and discloses a water plant virus separation method and application thereof, and the method comprises the step of mixing an amino-functionalized carbon nanomaterial and a water body containing plant viruses. The amino-functionalized carbon nanomaterial adopted by the method can be combined with plant viruses in a water body, so that the adsorption capacity of the amino-functionalized carbon nanomaterial to the plant viruses is improved, and the method is simple to operate, efficient and low in cost, does not generate toxic substances, avoids secondary pollution possibly caused by chemical disinfection, and is suitable for industrial production. The invention provides an economic and sustainable solution for solving the problem of plant virus pollution, and shows a wide application prospect in the field of water plant virus removal.

The present application relates to the technical field of aquatic plant virus separation, and discloses a method for separating aquatic plant viruses and application thereof, wherein the method comprises mixing amino-functionalized carbon nanomaterials and aquatic bodies containing plant viruses. The amino-functionalized carbon nanomaterials used in the method can be combined with the plant viruses in the aquatic bodies, thereby improving the adsorption capacity of the amino-functionalized carbon nanomaterials for the plant viruses. The method is simple, efficient and low in cost, and does not produce toxic substances, thereby avoiding secondary pollution possibly caused by chemical disinfection, providing an economic and sustainable solution to the problem of plant virus pollution, and showing a broad application prospect in the field of aquatic plant virus removal.