60 results about "Virus removal" patented technology

The invention provides a nylon 6 nanofiber virus-removing filtering membrane and a preparation method and application thereof.The nylon 6 nanofiber virus-removing filtering membrane comprises a base material and a nylon 6 nanofiber membrane compounded to the surface of the base material, the average pore size of the virus-removing filtering membrane is 20 nm, the virus-removing filtering membrane plays a role in blocking parvoviruses, the surface of the virus-removing filtering membrane is charged, and the surface of the virus-removing filtering membrane is not prone to being damaged. Proteins with the same charges on the surface can be prevented from adhering to the surface of the fiber. In the preparation process of the virus-removing filtering membrane, the nylon 6 nanofibers with a certain diameter range are selected and compounded on the surface of the base material in a stable dispersion state, the uniform microporous virus-removing filtering membrane is prepared, the effect that parvoviruses are blocked and protein molecules are not easily intercepted is achieved, the hydrophilicity of the nylon 6 nanofibers is relatively good, and the virus-removing filtering membrane has a good application prospect. The non-specific adsorption of the filtering membrane on the protein is reduced; the nylon 6 nanofiber virus-removing filtering membrane can be applied to the field of biological medicine products, and has important significance on separation and purification of biological preparations.

The application provides a virus-removing polyether sulfone porous membrane and a preparation method thereof. The virus-removing polyether sulfone porous membrane comprises, in sequence along a thickness direction, an outer layer region and an inner layer region, wherein the average pore size of the outer layer region is 10-20 nm, the thickness of the outer layer region is 20-50 microns, the average pore size of the inner layer region is 5-10 microns, and the thickness of the inner layer region is 60-150 microns; and the virus-removing polyether sulfone porous membrane is prepared by a method comprising at least the following process: taking polyether sulfone as a raw material, and obtaining the virus-removing polyether sulfone porous membrane under the action of a water-soluble diluent and a hydrophilic additive; wherein the water-soluble diluent comprises ethylene carbonate and polyethylene glycol. The virus-removing polyether sulfone porous membrane has a high virus removal rate and a high protein permeation rate.

The application belongs to the technical field of woody plant asexual propagation, and particularly relates to a method for establishing a tissue culture and rapid propagation system of Eucommia ulmoides stem segments. The method comprises the following steps: disinfecting Eucommia ulmoides seeds, obtaining sterile Eucommia ulmoides tissue culture seedlings through seed germination induction, cutting stems of the sterile Eucommia ulmoides tissue culture seedlings into stem segments with a length of 0.5 cm to 1 cm, inoculating the stem segments into an adventitious bud induction medium to perform adventitious bud induction culture, forming clustered adventitious buds after 20 days of the adventitious bud induction culture, separating the clustered adventitious buds into multiple individual adventitious buds, and transferring the individual adventitious buds into a rooting medium to perform rooting and seedling strengthening culture, forming a large number of adventitious roots after 10 days of the rooting and seedling strengthening culture, and obtaining complete regenerated Eucommia ulmoides plants. The method for establishing the tissue culture and rapid propagation system of Eucommia ulmoides stem segments has the advantages of short rapid propagation cycle, high rapid propagation efficiency, no seasonality limitation, good excellent traits maintenance, small space occupation, and virus removal.

A single-use valve arrangement including a valve housing, a diaphragm carried by the valve housing, a pressure sensor, and a controller coupled to the pressure sensor. The valve housing defines an inlet and an outlet, the inlet adapted to be fluidly connected to the outlet of the virus removal filter. The diaphragm divides the valve housing into a first chamber and a second chamber fluidly isolated from the first chamber. The pressure sensor is configured to measure a pressure change in the second chamber due to movement of the diaphragm in response to a pressure change in the first chamber. The controller is configured to determine an actual leak rate of the filter based on the measured pressure change, and the controller is further configured to determine the integrity of the filter by comparing the actual leak rate to an estimated leak rate of the filter.

This utility model provides a liquid flow channel structure for a virus removal filter, including an upper cover, several intermediate plates, and a lower cover connected in sequence. Compared with the prior art, the beneficial effects of this utility model are: 1. The venting ports of the pre-filter and post-filter chambers can completely exhaust the gas in the first pre-filter and first post-filter chambers, thereby preventing airlock on the filter membrane due to gas presence and avoiding affecting filtration efficiency; 2. The combination of straight and oblique flow channels, with the oblique flow channel outlet concentrated on one side, facilitates concentrated liquid flow into the second post-filter chamber, and also solves many problems in the filter manufacturing process, improving the utilization rate of the filter membrane; 3. The inlet buffer chamber and outlet buffer chamber allow the filter liquid to be spread smoothly and evenly on the filter membrane, avoiding the impact of liquid inflow on the filter membrane filtration effect.

The invention belongs to the technical field of membrane materials, and particularly relates to a preparation method of a dumbbell-shaped polyethersulfone virus-removing membrane, which comprises the following steps: S1, mixing a membrane-forming polymer, an amphiphilic polymer, a non-solvent pore-foaming agent and an organic solvent, stirring, dissolving and defoaming to obtain a uniform and stable membrane casting solution; s2, blade-coating the membrane casting liquid on a support body to form a liquid membrane; s3, exposing the liquid film in humidity-controllable air for a period of time, and carrying out a steam-induced phase separation process; s4, immersing the cast film obtained in the step S3 into a coagulating bath to complete phase inversion so as to form a solid-state film; s5, the formed solid membrane is sequentially subjected to water washing, hole preserving treatment and drying, and the dumbbell-shaped polyethersulfone virus-removing membrane is obtained. The compact layer in the dumbbell-shaped structure ensures the high virus rejection rate, and the porous structure with the open interior reduces the transmission resistance of water to the minimum, so that the unification of the high virus rejection rate and the high water flux is realized, and the protein transmittance is ensured to be more than 98%.

This invention discloses a method for virus-free tissue culture using terminal buds of *Prunus armeniaca* rootstock, belonging to the field of plant tissue culture technology. The method includes: selecting terminal buds of *Prunus armeniaca* rootstock for pretreatment, sterilizing them with ethanol and mercuric chloride, and then immersing them in a virus-free pretreatment solution containing ribavirin and triazole nucleoside for virus removal; inoculating the virus-free terminal buds into WPM-based differentiation induction medium to obtain clustered buds; culturing the clustered buds in 1 / 2 MS-based proliferation subculture medium to obtain proliferating buds; inoculating the proliferating buds into 1 / 3 MS-based rooting medium to induce rooting; and finally, after hardening off and transplanting into a vermiculite-garden soil-humus substrate for acclimatization. This invention optimizes the virus removal and tissue culture system based on the characteristics of *Prunus armeniaca* rootstock, achieving a virus removal rate ≥95%, a terminal bud differentiation survival rate ≥85%, a rooting rate ≥90%, and a transplant survival rate ≥88%. This enables rapid and large-scale propagation of virus-free *Prunus armeniaca* rootstock seedlings, solving the problem of low propagation efficiency in traditional methods and providing high-quality rootstock support for the *Prunus armeniaca* industry.

The invention belongs to the technical field of biology, and particularly relates to a linear tandem antigen epitope polypeptide of African swine fever virus RAN polymerase and application of the linear tandem antigen epitope polypeptide. The amino acid sequence of the linear tandem antigen epitope polypeptide is shown as SEQ ID NO.4 or SEQ ID NO.5; the linear tandem antigen epitope polypeptide is obtained by linearly connecting an epitope polypeptide NP1249L-1, an epitope polypeptide H359L-1 and an epitope polypeptide H359L-2 in series, the epitope polypeptide NP1249L-1, the epitope polypeptide H359L-1 and the epitope polypeptide H359L-2 are obtained through screening, and the linear tandem antigen epitope polypeptide has the functions of inducing ASFV specific T cells and assisting in controlling ASFV infection and removing viruses, and experimental results show that the linear tandem antigen epitope polypeptide has the advantages that the linear tandem antigen epitope polypeptide is a novel linear tandem antigen epitope polypeptide; the epitope polypeptide has the capability of inducing ASFV specific T cell response, and a theoretical basis is provided for subsequent development of polypeptide vaccines and diagnostic preparations based on ASFV protein source epitopes.

The invention discloses a duck plague virus LORF2 gene deleted strain as well as a construction method and application thereof, and belongs to the technical field of gene engineering. The construction method comprises the following steps: S1, by taking a pEP-Kan-S plasmid as a template, carrying out PCR (Polymerase Chain Reaction) amplification on a delta LORF2-Kan targeting fragment; s2, electrically transferring the targeting fragment to a competent state to construct a positive bacterium of which the LORF2 gene is replaced by a Kan resistance gene; s3, knockout of Kan resistance genes through homologous recombination; and S4, carrying out virus rescue to obtain the LORF2 gene deleted strain. The duck plague virus LORF2 gene deletion strain constructed by the invention has reduced pathogenicity to ducks and high immune factor level, can completely protect the ducks from being attacked by virulent viruses by immunizing the ducks with an ultra-low dose of 102 TCID50, has a virulent virus removal effect and a neutralizing antibody level which are similar to those of an existing commercial vaccine group, and provides strains, thoughts and technical supports for construction of DPV attenuated live vaccines.

The invention discloses skin mucosa maintenance liquid capable of killing fungi and HPV (human papillomavirus) as well as a preparation method and application of the skin mucosa maintenance liquid, and belongs to the technical field of sterilization and virus removal. The skin mucosa maintenance liquid is prepared from octanol ether, benzalkonium bromide, phenoxyethanol, yermate, parachlorometaxylenol, taetinic acid, chitosan, keratinase, fluconazole, glycerol, borneol, menthol, absolute ethyl alcohol and tween-40. Wherein the skin mucosa maintenance liquid has an effect of regulating apoptosis induction on abnormal cells and an effect on vaginal flora, and has an obvious inhibition effect on cervicitis and colpitis mycotica, namely an anti-inflammatory effect; meanwhile, apoptosis of cells containing HPV16 and HPV18 genes can be induced, shedding of abnormal cells is promoted, diseased cells are killed, and vaginal tissues with pathological changes are removed.

The invention belongs to the technical field of biology, and particularly relates to a T cell epitope polypeptide based on ASFV F1055L or P1192R protein, the T cell epitope polypeptide comprises a polypeptide F1055L-1, a polypeptide F1055L-2, a polypeptide F1055L-3 and a polypeptide P1192R-1, and the amino acid sequence is shown as SEQ ID NO.1-4. The polypeptide is obtained through screening and has the characteristics of inducing ASFV specific T cells and assisting in controlling ASFV infection and virus clearance. In-vitro experiments prove that the epitope polypeptide has the capability of inducing ASFV specific T cell response, and a theoretical basis is provided for subsequent development of polypeptide vaccines and diagnostic preparations based on ASFV protein source epitopes.

A chromatography device having a housing having an inlet and an outlet. At least two layers of media disposed between the inlet and the outlet inside of the housing forming a media stack, with at least one of the layers comprising a functionalized layer. An optional spacer ring disposed between the two layers of media forming an air gap between them. A non-functionalized sealing layer disposed between the inlet and the outlet inside of the housing as the last layer of media in the media stack within the housing as a fluid passes from the inlet to the outlet through the media stack. A margin of the sealing layer in contact with the housing; the margin being compressed by the housing forming a compressive seal to prevent fluid from leaking to the outlet past the compressive seal.

Provided in the present application are a virus removal filter membrane and a preparation method therefor, belonging to the technical field of membrane separation. The filter membrane preparation method comprises blending a hydrophilic polymer and a bulk polymer to prepare casting solutions having different viscosities, and on the basis of a double-layer homogeneous compounding method and a phase conversion method, preparing the filter membrane. The filter membrane has an integrated structure, and comprises a dense separation layer distributed along the thickness direction of the filter membrane and a pre-filter support layer having a loose sponge structure. The separation layer is adjacent to the support layer, but there is no obvious interface or stratification. The pore diameters of the separation layer and the support layer has a continuous gradient change along the thickness direction, wherein the pore diameter of the support layer is greater than the pore diameter of the separation layer.

The chromatography device has a housing with an inlet and an outlet. At least two media layers are disposed within the housing between the inlet and outlet, at least one of the layers having a functionalized layer. An optional spacer ring disposed between the two media layers forms an air gap therebetween. A non-functionalized sealing layer is disposed within the housing between the inlet and outlet as the last layer of media in the media stack within the housing as fluid passes from the inlet to the outlet through the media stack. A margin of the sealing layer contacting the housing is compressed by the housing to form a compression seal to prevent fluid from leaking past the compression seal to the outlet.

The present application relates to the technical field of aquatic plant virus separation, and discloses a method for separating aquatic plant viruses and application thereof, wherein the method comprises mixing amino-functionalized carbon nanomaterials and aquatic bodies containing plant viruses. The amino-functionalized carbon nanomaterials used in the method can be combined with the plant viruses in the aquatic bodies, thereby improving the adsorption capacity of the amino-functionalized carbon nanomaterials for the plant viruses. The method is simple, efficient and low in cost, and does not produce toxic substances, thereby avoiding secondary pollution possibly caused by chemical disinfection, providing an economic and sustainable solution to the problem of plant virus pollution, and showing a broad application prospect in the field of aquatic plant virus removal.

The application provides a PVDF porous membrane for removing viruses, a preparation method and a filter element, the porous membrane is made of PVDF material, comprises a main body, one side of the main body is a first porous surface, the other side of the main body is a second porous surface, the SEM measurement average pore size gradient of the main body is 2-12 nm / μm; the pore size gradient of the porous membrane is small; in the wet state of the porous membrane main body, the part of capturing 20 nm colloidal gold is D20, D20 is the area from the first porous surface and located at 20%-100% of the film thickness, part of the 20 nm colloidal gold is located at the second porous surface; the thickness of D20 is 15-35 μm; the porous membrane has good interception effect on 20 nm colloidal gold; meanwhile, the pore size of the small pore area is large, so that the membrane flux is high and the flux attenuation is slow, the mechanical strength is high, and the porous membrane is particularly suitable for filtering high-concentration protein fluid; the LRV value of the porous membrane for 20 nm colloidal gold is greater than 2 and less than 4, the double-layer stacking is used in actual use, and the risk of virus leakage is avoided; the preparation method is simple, fast, effective, green and environment-friendly.

The invention discloses a technology for removing viruses from tetanus human immune globulin through a nano film. The process comprises the following steps: pretreating a feed liquid, so that the protein concentration of the feed liquid is 50-60g / L, the pH is 3.8-4.4, and the feed liquid contains 90-110g / L maltose and 50-150mmol / L sodium chloride; then, the feed liquid is subjected to two-stage filtration through a 0.1-micron nylon pre-filtration membrane bag with negative charges and a 20-nm regenerated cellulose (RC) virus removal membrane bag in sequence. According to the process, through optimizing the physical and chemical state of the feed liquid and the synergistic effect of the feed liquid and the functionalized filter membrane, the protein aggregation is effectively inhibited, the membrane pollution is reduced, the virus removal effect is ensured not to be lower than 4log10, the protein load of the RC membrane is remarkably improved to be more than 200L / m, and the product purity is more than or equal to 98%. The invention solves the technical problems of low flux and easy blockage of high-concentration immune globulin in small-aperture virus removal filtration, and is suitable for efficient, safe and large-scale production of blood products.

This application describes an asymmetric cellulose virus removal filtration membrane comprising a porous body having a liquid supply surface and a liquid discharge surface. The average pore size measured by SEM is larger on the liquid supply surface than on the liquid discharge surface. The area in the filtration membrane where gold colloids with a particle size of x nm are captured is defined as Dx, and D 20 The region is located within 20-99% of the area of ​​the porous body, and the ratio of its thickness to the thickness of the porous body is 15-40%, and D 40 The present application also discloses an asymmetric cellulose virus removal filtration membrane, in which the region is located within 0-80% of the porous body, the ratio of the thickness of the region to the thickness of the porous body is 30-70%, and the interior of the filtration membrane further includes a transition region having a thickness of 20 μm or less, and a preparation process for the filtration membrane. The filtration membrane of the present application uses a cellulose-based raw material with excellent hydrophilicity as the membrane-forming material, has a distance between the 20 nm gold colloid trap region and the drainage surface, the thickness of the 40 nm gold colloid trap region is large, and the thickness of the transition region inside the filtration membrane is small, resulting in a high virus trapping rate, high load capacity, and high protein yield.

The invention provides a method for removing tomato spotted wilf viruses in tomato seeds. The method comprises the following steps: (1) soaking the tomato seeds with an ozone aqueous solution; and (2) performing soaking treatment on the tomato seeds subjected to soaking treatment in the step (1) by using a detoxification agent composition so as to obtain the tomato seeds without the tomato spotted wilf virus. According to the removal method provided by the invention, the tomato spotted wilf virus carried by the tomato seeds can be effectively removed, the germination rate of the tomato seeds is not reduced, and the problems that the virus removal efficiency is low and the vitality of the tomato seeds is reduced in the prior art are solved. Moreover, the method is economical, efficient, environment-friendly and simple and convenient to operate, has remarkable popularization value, can provide reliable technical support for tomato virus disease prevention and control and non-toxic seedling breeding, and has long-term significance for guaranteeing cross-regional healthy circulation of seeds and agricultural safety production.

The utility model discloses an ultrafiltration membrane for separating viruses and proteins, and belongs to the technical field of virus-removing flat membranes and virus-removing filters. Comprising a spongy macroporous layer, a non-woven fabric layer tightly connected with the spongy macroporous layer, and a spongy microporous layer connected with the non-woven fabric layer, the spongy macroporous layer completely permeates into the non-woven fabric layer, and the spongy microporous layer partially permeates into the non-woven fabric layer. The structure is integrally formed, large-scale preparation can be achieved, meanwhile, film material layers are tightly combined, the falling risk is avoided, the enhanced structure strength is high, and the risks of damage and leakage in the assembly preparation process are greatly reduced.

The present invention relates to artificial antigen presenting cell (aAPC) scaffolds to provide cells with specific functional stimulation to obtain phenotypic and functional properties ideal to mediate tumor regression or viral clearance. In particular, the scaffolds of the present invention comprise stabilized MHC class I molecules comprising a heavy chain comprising an alpha-1 domain and an alpha-2 domain connected by a disulfide bridge, wherein said MHC class I molecules are free of antigenic peptide. The scaffolds can be loaded with antigenic peptide on demand, providing an agile platform for effective expansion and functional stimulation of specific T cells in a peptide-MHC-directed fashion.

The present application provides a virus detoxification method of Chinese yam. The present application can effectively prevent Japanese yam mosaic virus (JYMV), potato virus Y (PVY), potato virus X (PVX) and potato leaf roll virus (PLRV) by peeling the stem tip of Chinese yam and using heat treatment and ribavirin detoxification. The optimal virus removal method is to peel the stem tip to 0.3-0.5mm, heat treatment at 40℃ for 8h, and ribavirin 50mg / L.

The application discloses a preparation method of rabbit round small sac transfer factor, comprising the following steps: 1) mixing water for injection with ground rabbit round small sac, and homogenizing the mixture by a colloid mill to obtain a homogenate; 2) centrifuging the homogenate after cell crushing, performing tangential flow filtration on the harvested supernatant, and collecting the permeate; 3) performing tangential flow filtration on the permeate by using filter membranes with different molecular weight cut-off pore sizes in stages to obtain crude products of rabbit round small sac transfer factor with required molecular weight ranges; 4) mixing at least one crude product with a certain molecular weight range in step 3) to obtain a primary product of rabbit round small sac transfer factor; and 5) adjusting the pH and the osmotic pressure of the primary product of rabbit round small sac transfer factor after virus removal, performing sterilization filtration, and obtaining rabbit round small sac transfer factor. The application adopts the tangential flow filtration in stages, establishes precise and effective separation and concentration technology of rabbit round small sac transfer factor and small molecule purification technology, and can prepare rabbit round small sac transfer factors with various molecular weight ranges according to different technological process flows by selecting filter membranes with different molecular weight cut-off pore sizes.

The present invention relates to a method for manufacturing a hydrophilic microporous membrane for virus filtration. Specifically, the hydrophilic microporous membrane is manufactured by performing a hydrophilization treatment without using a crosslinking agent, and the microporous membrane for virus removal thus manufactured has excellent water permeability and protein permeability and exhibits good durability against organic solvents.

The invention relates to a human immunoglobulin product for intravenous injection, which is prepared by virus removal and filtration of a 20nm nano-film, and the nano-filtration protein load is more than 10kg / m < 2 >. The preparation method of the product comprises the following steps: after the intravenous injection human immune globulin stock solution is prepared and before 20nm nano-film virus removal filtration is carried out, adjusting the glycine content in the protein stock solution to 20g / L, adjusting the sodium acetate concentration to 20mmol / L, adjusting the protein content to 58g / L and adjusting the pH value to 4.1, and then carrying out 20nm nano-film virus removal filtration. According to the method disclosed by the invention, the nano-filtration protein load is effectively improved by more than 10kg / m < 2 > protein through the measures of optimizing a stabilizer in a stock solution before filtration of the human immunoglobulin nano-membrane for intravenous injection, adjusting the ionic strength and the like, so that the use efficiency of the nano-filtration membrane is improved, and meanwhile, the virus removal filtration process time of the nano-membrane is also shortened.

This application provides a method for characterizing and / or determining the viral removal capacity of hydrophobic interaction chromatography (HIC), including an experimental design for multivariate analysis of viral removal by HIC. The method provides an understanding of the mechanism of viral removal using HIC by executing a D-Optimal experimental design that includes evaluations of multiple factors, such as pH, buffer concentration, column loading concentration, column flow rate, and hydrophobic strength of the HIC column.

The invention belongs to the technical field of biological detection, and particularly relates to a method and a kit for detecting the influenza virus removal rate of an air purifier and application of the kit. The detection method comprises the following steps: S.1, preparing virus aerosol; s.2, carrying out initial sampling; s.3, sampling again; s.4, virus RNA (Ribonucleic Acid) extraction: extracting RNA of the collected virus liquid by using a TRIzol method; s.5. RT-qPCR amplification reaction: extracting single virus RNA by using a fluorescent probe and a specific primer pair, and carrying out RT-qPCR amplification reaction; s.6, result judgment: if Ct in an FAM channel is less than or equal to 37 and an amplification curve is S-shaped, the signal is a positive signal, and if no amplification curve exists or Ct is greater than 40, the signal is a negative signal; and S.7, calculating the virus removal rate. The RT-qPCR technology is adopted, rapid amplification and detection of virus nucleic acid are achieved, the detection period is greatly shortened, the detection efficiency is improved, and a low-concentration virus sample can be accurately detected. Non-specific amplification is effectively avoided, and the accuracy of a detection result is ensured.

The invention relates to a method for establishing an elm leaf tissue culture rapid propagation system, which belongs to the technical field of woody plant asexual propagation, and comprises the following steps: firstly, selecting an elm branch which grows robustly and is free from plant diseases and insect pests as an explant, and inducing the elm branch to generate adventitious roots; then, taking leaves of sterile elm tissue culture seedlings as materials, inoculating the leaves into a callus induction culture medium for callus induction culture, transferring the leaves into an adventitious bud induction culture medium with an optimized proportion, and performing induction culture for 30 days to form clustered adventitious buds; transferring the adventitious buds into a rooting culture medium for rooting induction and strong seedling culture, and finally obtaining an elm regenerated plant which has a complete root system and grows robustly. The establishment method of the elm leaf tissue culture and rapid propagation system has the advantages of being short in rapid propagation period, high in rapid propagation efficiency, not limited by seasons, capable of maintaining excellent characters, small in occupied space and capable of removing viruses.

Described herein are methods for viral clearance of samples comprising proteins using affinity chromatography. Methods include contacting an affinity chromatography resin with a liquid sample comprising protein and virus, or suspected of having virus, wherein the affinity chromatography resin comprises a cross-linked polysaccharide with a Protein A functional group and the protein comprises an Ig sequence. Methods include eluting the protein from the affinity chromatography resin with an elution liquid having a pH of about 4 to about 5.5 to produce an eluted sample, whereby the eluted sample has a reduction in virus compared to the liquid sample.

The present invention relates to a method for manufacturing a hydrophilic microporous membrane for virus filtration. Specifically, the hydrophilic microporous membrane is manufactured by performing a hydrophilic treatment without using a crosslinking agent, and the microporous membrane for virus removal thus manufactured has excellent water permeability and protein permeability and exhibits good durability against organic solvents (alcohols).