64 results about "Concentration protein" patented technology

The invention discloses a plant immune induced resistance protein PpAE4 and application thereof, and the protein can induce plant resistance and can be used as a plant immune activating factor to be applied to the field of prevention and treatment of plant diseases. The amino acid sequence of the protein is as shown in SEQ ID NO: 2. High-concentration protein can be obtained through expression of the engineering strain, the disease resistance of plants to soybean rust germs can be improved when the protein is used for treating the plants, and the protein is low in needed concentration, quick in effect taking and long in acting time. The PpAE4 provides a new way for improving plant disease resistance and preventing and treating plant diseases.

The invention discloses a protein recovery device for chondroitin sulfate production wastewater, and relates to the technical field of sewage recovery, and the technical scheme is characterized in that a separation assembly is used for separating mixed-concentration protein liquid entering the device and comprises a rotational flow layer, the rotational flow layer is used for preliminarily separating low-concentration and high-concentration protein liquid, and a U-shaped pipe and a transmission mechanism are arranged in the rotational flow layer; the device comprises an impeller arranged at the bottom of one end, located outside a rotational flow layer, of a U-shaped pipe, and a scraper located in the screening layer, and has the effects that manual interference is not needed, driving is completed through the natural effect, and under the condition that a protein screen is blocked, the blockage of the protein screen is avoided. And the impeller automatically drives the scraper to scrape and clean the inner wall of the protein screen, so that the problem of equipment blockage in the background art is solved, the frequency of manual operation is reduced, and the collaboration of the device and the working procedure efficiency of low-concentration and high-concentration protein separation and recovery are improved.

The present disclosure provides a process for concentrating proteins including an ultrafiltering, a diafiltering, and a second ultrafiltering sequence, at elevated temperatures, such as above about 30° C. The disclosure also includes a process for preparing highly concentrated antibody compositions, and highly concentrated antibody products.

As a series of viscosity-reducing and stabilizing formulations for high-concentration protein formulations, they are finely tuned to significantly reduce the viscosity of high-concentration biopharmaceuticals and increase their stability. Viscosity-reducing and stabilizing formulations can be used for high-concentration biopharmaceutical products and have significant potential to improve stability during manufacturing and storage, thereby increasing the feasibility of high-concentration biopharmaceutical products.

The invention discloses a method for preparing protein concentrate from high-denaturation oilseed meal, and belongs to the technical field of edible protein processing. The method comprises the following steps: crushing and screening to obtain meal powder with different protein contents by utilizing the distribution rule of proteins and structural carbohydrates in the microstructure of the high-denaturation oilseed meal and the physical property characteristics of components, and carrying out graded processing treatment according to the property difference of the meal powder; an air-electric field separation device is used for treating large-particle oilseed meal, high-fiber components which are difficult to remove in a traditional process are separated out, and high-protein components with solubility (NSI) similar to that of low-denaturation soybean meal and high gel property are obtained; proper concentration or alcoholase treatment is selected according to the protein content, denaturation degree and structural characteristics of the screening component, and a concentrated protein product meeting specific protein content requirements and / or functional characteristics can be obtained.

The invention provides an electrode for detecting protein substances, a preparation method of the electrode, a biosensor and a microflow detector. The electrode comprises a carbon electrode, a Prussian blue layer and a gold nanoparticle layer which are stacked in sequence, the side, away from the Prussian blue layer, of the gold nanoparticle layer is modified with an aptamer, the particle size of gold nanoparticles of the gold nanoparticle layer ranges from 20 nm to 100 nm, and the mass of the gold nanoparticles on the Prussian blue layer per unit area ranges from 4 micrograms / cm < 2 > to 5 micrograms / cm < 2 >; the Prussian blue layer is formed on one side of the carbon electrode by adopting a cyclic voltammetry method, the cyclic voltammetry method adopts a three-electrode system to perform continuous scanning for 20-40 circles in a potential range of-0.2 V-0. 9V, an electrolyte comprises 5-25 mM of potassium ferricyanide, 5-25 mM of ferric chloride, 0.12-0.25 M of hydrochloric acid, 5-50 mM of potassium chloride and the balance of water, and the molar ratio of the potassium ferricyanide to the ferric chloride is 1: 1. The electrode has more excellent sensitivity and stability, and detection of pg / mL-magnitude ultralow-concentration protein substances is realized.

The application discloses a plant immunity elicitor protein PpAE4 and application thereof, the protein can induce plant resistance, and can be applied to the field of plant disease prevention and treatment as a plant immunity activator. The amino acid sequence of the protein is shown as SEQ ID NO:2. High-concentration protein can be obtained by using an engineering strain for expression, and the protein can be used for treating plants to improve the disease resistance of the plants to soybean rust fungi, and the required concentration is low, the effect is quick, and the action time is long. The PpAE4 provides a new way for improving the disease resistance of plants and preventing and treating plant diseases.

The invention provides a solvent dosage accurate regulation and control method for preparing dephenolized cottonseed protein concentrate, which comprises the following steps: shelling, crushing and determining indexes of a cottonseed sample to obtain stable spectrum data and basic label data, and selecting southern and northern Xinjiang mixed cottonseeds for difference analysis to obtain basic processing frequency parameters; based on basic processing frequency parameters, converting total solvent dosage and content rules, preparing dephenolized concentrated cottonseed protein, and performing quality analysis to obtain a quality evaluation result; carrying out correction, exception processing, preprocessing, standardization and data set division on the stable spectral data to obtain a modeling data set; and constructing a hyperspectral quantitative correction model to obtain the predicted solvent dosage, and carrying out cross validation on the hyperspectral quantitative correction model. According to the method, the consumption of the reagent in the extraction process of preparing the dephenolized cottonseed protein concentrate is dynamically regulated and controlled through the model, so that the accurate estimation and regulation and control of the reagent consumption in the treatment process of different varieties of cottonseeds are realized.

The invention relates to diagnostic methods, and particularly, although not exclusively, to the use of oligomeric protein traps in sample diagnostics. The invention extends to the use of oligomeric protein traps as binding agents, for example in methods for enriching samples of low-concentration proteins and / or genetic material, and methods for detecting the same in samples. The invention also encompasses high-affinity protein traps and their application in environmental monitoring, diagnosis and therapy.

The invention discloses a soybean protein concentrate milk beverage and a preparation method thereof, and belongs to the field of food processing. According to the invention, the problem of low solubility of the soybean protein concentrate at present is solved, and the application of the soybean protein concentrate in the beverage market is expanded. The preparation method of the soybean protein concentrate milk beverage comprises the following steps: modifying soybean protein concentrate powder by using an ultrasonic technology to improve the solubility of soybean protein concentrate, then adding xanthan gum to improve the emulsibility and stability of soybean protein concentrate milk, and mixing the soybean protein concentrate milk with ingredients through high-pressure homogenization to obtain the soybean protein concentrate milk beverage. According to the method, the application range of the soybean protein concentrate is expanded, and the soybean protein concentrate can be widely applied to the field of food processing.

The invention discloses abalone feed with gulfweed powder partially replacing kelp powder and a preparation method of the abalone feed, and belongs to the technical field of cultivation. The abalone feed is prepared from the following components in percentage by weight based on the total weight of the feed: 10% of gulfweed powder, 20% of kelp powder, 20% of high gluten flour, 20% of soybean meal, 20% of shell powder, 20% of fish meal, 10% of soybean protein concentrate, 10% of flour, 10% of monocalcium phosphate, 20% of choline chloride, 10% of fish oil, 10% of soybean oil, 10% of vitamin premix and 10% of mineral premix. By reasonably designing the proportion of the gulfweed powder and the kelp powder in the abalone feed formula, the feed preparation cost and abalone feeding cost can be effectively saved, meanwhile, the abalone feed has no significant influence on abalone growth, and healthy and sustainable development of marine fishery resources is facilitated.

Provided herein are methods for producing a mung bean protein isolate having high functionality for a broad range of food applications. In some embodiments, the methods for producing the isolate comprise one or more steps selected from: (a) extracting one or more mung bean proteins from a mung bean protein source in an aqueous solution, for example, at a pH between about 6.5-10.0; (b) purifying protein from the extract using at least one of two methods: (i) precipitating protein from the extract at a pH near the isoelectric point of a globulin-rich fraction, for example a pH between about 5.0-6.0; and / or (ii) fractionating and concentrating protein from the extract using filtration such as microfiltration, ultrafiltration or ion-exchange chromatography; and (c) recovering purified protein isolate.

The application provides a SERS method for detecting protein based on a sandwich method combining gold nanoparticle probes and antibodies, wherein the method can realize high sensitivity and high selectivity by functionalizing specific antibodies on the surface of gold nanoparticles and combining a sandwich detection strategy, is suitable for detecting ultra-low concentration protein, and has the potential to be expanded to multi-marker detection, and by selecting Raman reporter molecules with characteristic peak separation, signal overlap and background interference can be effectively reduced.

The invention discloses a production process of high-purity low-fat animal protein powder, which comprises the following steps: S1, selecting animal fat, and carrying out pretreatment of impurity removal and cleaning; s2, the pretreated raw materials are put into closed cooking equipment for high-temperature and high-pressure cooking, and oil-water protein three-phase layering occurs; s3, collecting a middle-layer protein liquid phase, and performing refined filtration to obtain refined protein liquid; s4, performing vacuum concentration on the refined protein liquid to obtain concentrated protein liquid; and S5, carrying out spray drying on the concentrated protein liquid to prepare an animal protein powder finished product, wherein the protein content of the finished product is greater than or equal to 90%. Compared with the prior art, the production process has the advantages that a production method based on combination of high-temperature cooking vaporization, physical layering and spray drying is provided, and the production process of the high-purity low-fat animal protein powder with the protein content larger than or equal to 90% is achieved.

The invention relates to a method and a device for enhancing immunoblotting and immunoblotting electrophoresis sensitivity. The method comprises the following steps: performing regular stripe standing wave concentration on protein by utilizing regular stripe standing waves generated by superposing surface sound source ultrasonic waves through a gel electrophoresis carrier which is subjected to electrophoretic separation and contains a low-concentration protein molecule band in a direction vertical to electrophoretic separation; and the protein molecules with different densities are pushed to the node or antinode region of the standing wave and are concentrated. The device comprises an area sound source generator operated by the method and an echo carrier plate with an echo plate. An electrophoresis tank for accommodating an echo carrying disc is additionally arranged, so that the electrophoretic separation step and the concentration step are integrated; and a main bearing body is arranged for bearing the electrophoresis tank, a related power supply and a related switch. According to the method and the device, the concentration of the low-abundance protein in the reaction area reaches or is closer to the optimal concentration for antigen-antibody reaction, in the immunoblotting process, the local luminescence or fluorescence peak intensity after membrane transfer meets the condition of reaching a threshold value or above, and the detection rate of the low-abundance target protein is greatly improved.

The invention belongs to the technical field of soybean processing, and particularly relates to a soybean protein concentrate processing technology which comprises the following steps: step 1, raw material pretreatment and acid precipitation; step 2, dehydration treatment; step 3, drying treatment; step 4, dedusting and conveying; step 5, packaging; raw material pretreatment and acid precipitation are as follows: low-temperature squeezed soybean cake powder is put into a stirring tank, treated pure hot water with the temperature being 50-55 DEG C and the volume being 7 times that of the materials is added into the tank, meanwhile, citric acid is added and stirred until the materials are fully dissolved, and the materials are soaked and stirred for 40-45 minutes, so that water-soluble components in the materials are fully dissolved. The method solves the problems of low raw material utilization rate, difficulty in product activity control and byproduct waste in the traditional process, realizes efficient preparation of the soybean protein concentrate and recycling of byproducts, and improves production benefits and product quality.

The present disclosure relates to formulations comprising a protein having an Fc region (e.g., an antibody) and a surfactant such as polysorbate (e.g., polysorbate 20), and methods of purifying the protein that improve the stability of the surfactant such as polysorbate in the formulation.

The utility model provides an ultrafiltration concentration tube device with a sterile filtration function, which solves the problems that the existing ultrafiltration tubes with different specifications and models can only achieve the purpose of protein concentration, different proteins can be confused due to frequent operation after the concentration is finished, the recovery rate is reduced, the risk of introducing bacteria is increased and the like. According to the main scheme, a tube body internally provided with a cavity is included, the top of the tube body is open, the tube body can be sealed into a whole through a tube cover, the tube body is formed by splicing three sections, the adjacent splicing positions are fixed through deformation friction, and the three sections of the tube body sequentially comprise an antibody storage area, a liquid storage area and a liquid discharging area from the upper end opening to the lower end opening; a first supporting table and a second supporting table are fixed to the two sequentially-connected positions of the inner wall of the pipe body respectively, a sterile filter membrane is embedded in the first supporting table, and an ultrafiltration membrane is embedded in the second supporting table.

The application relates to a compound soybean concentrated protein, which comprises the following components: 50-72 parts of soybean protein isolate, 4-13 parts of soybean dietary fiber, 20-25 parts of soybean powder, 0.5-0.8 parts of ethyl maltol, 21-27 parts of water-soluble coating taste-improving particles, and 2-3 parts of an emulsifier, wherein the water-soluble coating taste-improving particles comprise a coating wall mixture and a core mixture, and the mass ratio of the coating wall mixture to the core mixture is 5:1. The beneficial effects are as follows: compared with a product made of traditional compound concentrated protein, the product has significant improvement in elasticity and hardness, the mixing time with sausage meat is shortened, the mixing production efficiency is improved, the product has the advantages of being delicate and glossy, not sticking to sausage casing, lubricated taste, high hardness, good elasticity, no water and oil seepage phenomenon, high yield, good water and oil retention effect, and the like, the edible spices are stored in a closed manner, and are mixed into the sausage at last during mixing and stirring, the sausage has more sufficient fragrance, the yield is improved, and the taste of the product is improved.

The invention discloses a solid-phase extraction-liquid chromatography detection method for organic acid salts in assisted reproduction liquid, and relates to the field of in-vitro assisted reproduction liquid detection, the method comprises the following steps: (1) dissolving and diluting the organic acid salts with a mobile phase respectively to obtain a reference substance stock solution; (2) adding an eluent into the reference substance stock solution, carrying out nitrogen blowing concentration, and fixing the volume by using a mobile phase to obtain a reference substance solution; (3) mixing the assisted reproduction liquid and the treating agent, and incubating to obtain a pretreated test sample; performing solid-phase extraction on the pretreated test sample to obtain a test sample solution; and (4) injecting the blank solution, the test solution and the reference solution into a high performance liquid chromatograph, and testing under a chromatographic condition. According to the method, pretreatment and solid-phase extraction technologies are combined, so that the matrix effect of impurities such as high-concentration protein in detection of organic acid salts in the assisted reproduction liquid is reduced, instruments are protected, the selectivity of the detection method is improved, and the accuracy of qualitative and quantitative detection is improved.

Disclosed herein are methods for determining a concentration of at least one low concentration protein and at least one high concentration protein in a biological sample.

The utility model relates to the technical field of milk protein production, in particular to a membrane filtration system for producing high-activity whole milk protein powder, which comprises an ultrafiltration membrane group, a nanofiltration membrane group and a backwashing component, the ultrafiltration membrane group comprises a first ultrafiltration membrane group and a second ultrafiltration membrane group, and the second ultrafiltration membrane group is arranged behind the first ultrafiltration membrane group; the backwashing assembly is arranged between the first ultrafiltration membrane group and the second ultrafiltration membrane group, and an outlet of the backwashing assembly is connected with an inlet of the second ultrafiltration membrane group; the nanofiltration membrane group is arranged behind the second ultrafiltration membrane group. By adopting the membrane filtration system combining the ultrafiltration membrane group and the nanofiltration membrane group, the protein content in the finally produced concentrated protein liquid can be increased, and the backwashing assembly can take away more lactose, so that the protein content is increased, and the protein content in the finally produced concentrated protein liquid reaches 25%.

The present invention discloses a method for quantitatively detecting amino acids in high-concentration protein preparations without derivatization. The method uses a graphitized carbon chromatographic column for separation, adds the ion-pairing reagent trifluoroacetic acid to the mobile phase, and employs a charged aerosol detector (CAD) for detection. Multiple amino acid standard solutions with varying concentrations ranging from 1 mmol / L to 20 mmol / L are prepared. A quadratic standard curve is plotted, representing the relationship between amino acid standard concentration and peak area, to serve as the detection equation for amino acid concentration. The test sample is diluted multiple times with ultrapure water, then ultrafiltered and centrifuged for protein removal before injection. The corresponding peak area is then integrated from the test sample's chromatographic profile and then substituted into the detection equation to determine the amino acid concentration in the test sample. This method achieves high detection accuracy, high efficiency, and simple operation, while significantly reducing the adverse effects of high-concentration protein on the chromatographic column.

The present invention relates to compositions of highly concentrated protein formulations showing reduced viscosity. The contained proteins in the prepared formulations are stabilized against aggregation and denaturation and are thus sufficiently storage-stable until administration to the patient.

The invention relates to a method for separating and preparing micro-molecule polypeptide from municipal sludge and recycling the micro-molecule polypeptide, which comprises the following steps: obtaining high-purity protein through the technological processes of concentration, purification and the like, further hydrolyzing to generate the micro-molecule polypeptide and recycling the micro-molecule polypeptide. The micromolecule polypeptide is prepared through the five stages of pretreatment, sludge pyrolysis, protein extraction and concentration, protein separation and purification and enzymolysis conversion, the micromolecule polypeptide with extremely high value can be obtained, and other generated by-products such as residual solid powder and residual nutrient solution can be used in the fields of building material industry, soil improvement and the like; and resource comprehensive utilization is realized.

The present application relates to the technical field of feed processing, and discloses a kind of high-protein straw compound feed and preparation method thereof, solve straw burning pollution, and the technical problem of low breeding efficiency and single nutrition of pure straw feed.The present application uses agricultural straw as main material, and innovatively introduces high-concentration protein mother liquor produced by grain deep processing enterprises as core nutritional supplement.The mother liquor is rich in natural protein, amino acids, minerals, vitamins and other core nutrients required for animal growth, which can effectively replace part of artificial feed additives and has the effect of promoting animal growth.The feed prepared by the present application can ensure that the breeding efficiency is not lower than or slightly lower than that of pure grain feed, and can minimize the harmful component residues in the animal body;At the same time, it realizes the efficient resource utilization of agricultural straw, reduces straw burning pollution from the root, reduces feed production cost, realizes the reutilization of grain deep processing byproducts, and can be widely used in various livestock and poultry breeding.

The invention particularly relates to a composite functional pet phagostimulant and a preparation method thereof.The preparation method comprises the following steps that 1, after chicken liver pulp, chicken oil and concentrated protein liquid are subjected to enzymolysis, zymolyte is collected; (2) carrying out Maillard reaction on the zymolyte obtained in the step (1), yeast hydrolysate and reducing sugar step by step; (3) after cooling in the step (2), adding a nutrition enhancer, an intestinal health agent, an antioxidant and a stabilizer, and homogenizing and stabilizing; and (4) adding an acidity regulator and a preservative into the mixture obtained in the step (3), uniformly mixing, filling, sterilizing and packaging. The raw material of the added concentrated protein liquid is cooking residual water of meat, comprehensive utilization can reduce the pressure of wastewater treatment, meanwhile, more nutrient substances are added to the phagostimulant, functional additives in the phagostimulant are stably embedded, the antioxidant slow-release effect and the intestinal tract targeted release effect of prebiotics are enhanced, and the nutritional value of the phagostimulant is improved. The added value of the phagostimulant is improved, and the phagostimulant has a good commercial application prospect.

A process for producing a fermentation product and a concentrated protein co-product from a starch-containing material.

Methods of producing concentrated protein products from non-animal organic matter, such as seaweed, are described herein. According to some implementations, the method includes obtaining the organic matter, which can include algae. In some implementations, the method includes creating a suspension containing the organic matter, and treating the suspension with an enzyme mixture. Some implementations include extracting a high-protein concentrate from the suspension after treatment with the enzyme mixture. Other implementations are also described.

The invention discloses a crucian feed with soybean meal replaced by cottonseed protein concentrate and application of the crucian feed. Cottonseed protein concentrate is used as a main plant protein source and completely replaces soybean meal in a feed formula, and the mass percent of the cottonseed protein concentrate is 19.78%. The feed further comprises the following components: fish meal, casein, flour, fish oil, soybean oil, vitamins, mineral premix and the like. The feed provided by the invention can significantly improve the ingestion speed and the ingestion amount of crucian carps and effectively promote the growth of crucian carps, and the weight gain rate, the specific growth rate and the feed efficiency of the feed are significantly superior to those of a feed containing soybean meal. The feed can regulate and control lipid metabolism related gene expression of crucian muscle and liver, enhance fat synthesis and catabolism, promote muscle protein and fat deposition and increase muscle polyunsaturated fatty acid content, and has no negative influence on liver health and intestinal flora homeostasis. The invention provides an efficient solution for soybean meal decrement substitution, and has important application value.