82 results about "Concentration protein" patented technology

The invention discloses a non-derivatization quantitative detection method for amino acid in a high-concentration protein preparation, which comprises the following steps: separating by using a graphitized carbon chromatographic column, adding an ion pair reagent trifluoroacetic acid into a mobile phase, and detecting by using an electric fog detector (CAD); the method comprises the following steps: preparing a plurality of groups of amino acid standard substance solutions with different concentration groups, wherein the concentration range is 1mmol / L-20mmol / L; drawing a quadratic equation standard curve of the amino acid standard product concentration and the peak area as a detection equation for detecting the amino acid concentration; the method comprises the following steps: diluting a test sample by using ultrapure water for multiple times, carrying out ultrafiltration centrifugal treatment, removing protein, introducing the sample, obtaining a corresponding peak area according to the spectrum integral of the test sample, and substituting the peak area into the detection equation to obtain the concentration of amino acid in the test sample. The method has the advantages of high detection precision, high detection efficiency and simple operation, and greatly reduces the adverse effect of high-concentration protein on the chromatographic column.

The invention discloses a plant immune induced resistance protein PpAE4 and application thereof, and the protein can induce plant resistance and can be used as a plant immune activating factor to be applied to the field of prevention and treatment of plant diseases. The amino acid sequence of the protein is as shown in SEQ ID NO: 2. High-concentration protein can be obtained through expression of the engineering strain, the disease resistance of plants to soybean rust germs can be improved when the protein is used for treating the plants, and the protein is low in needed concentration, quick in effect taking and long in acting time. The PpAE4 provides a new way for improving plant disease resistance and preventing and treating plant diseases.

The invention discloses a protein recovery device for chondroitin sulfate production wastewater, and relates to the technical field of sewage recovery, and the technical scheme is characterized in that a separation assembly is used for separating mixed-concentration protein liquid entering the device and comprises a rotational flow layer, the rotational flow layer is used for preliminarily separating low-concentration and high-concentration protein liquid, and a U-shaped pipe and a transmission mechanism are arranged in the rotational flow layer; the device comprises an impeller arranged at the bottom of one end, located outside a rotational flow layer, of a U-shaped pipe, and a scraper located in the screening layer, and has the effects that manual interference is not needed, driving is completed through the natural effect, and under the condition that a protein screen is blocked, the blockage of the protein screen is avoided. And the impeller automatically drives the scraper to scrape and clean the inner wall of the protein screen, so that the problem of equipment blockage in the background art is solved, the frequency of manual operation is reduced, and the collaboration of the device and the working procedure efficiency of low-concentration and high-concentration protein separation and recovery are improved.

The present disclosure provides a process for concentrating proteins including an ultrafiltering, a diafiltering, and a second ultrafiltering sequence, at elevated temperatures, such as above about 30° C. The disclosure also includes a process for preparing highly concentrated antibody compositions, and highly concentrated antibody products.

The invention discloses a baked pudding which comprises the following components in parts by weight: 600-650 parts of cow milk, 60-80 parts of yolk, 200-250 parts of single cream, 4-6 parts of whey protein powder and 10-15 parts of milk protein concentrate. Under the condition that colloid additives (such as carrageenan, gelatin and the like) are not added, the whey protein powder and the milk protein concentrate are compounded into the pudding components, so that the prepared baked pudding not only has excellent congelation strength (the congelation strength reaches 35-40g), but also has a stable product system within the shelf life (within 21 days), and is suitable for industrial production. And the phenomena of water separation and product breakage are avoided.

The invention provides a novel compound feed for leuciscus waleckii, which comprises the following components: 10 to 30 percent of fish meal, 8 to 12 percent of soybean protein concentrate, 15 to 20 percent of flour, 3 to 6 percent of casein, 0.5 to 2 percent of gelatin, 4 to 6 percent of corn protein powder, 2 to 3 percent of soybean oil, 2 to 3 percent of fish oil, 18 to 24 percent of dextrin, 0.3 to 0.6 percent of mineral substance and vitamin premix, 0.2 to 0.6 percent of choline chloride, 1.5 to 2.5 percent of monocalcium phosphate, 0.05 to 0.12 percent of vitamin C ester, 2.5 to 3.5 percent of microcrystalline cellulose and the balance of water. The non-grain novel protein source defatted tenebrio molitor powder is used for partially replacing the fish meal, and the replacement proportion is 0-60%. The invention further provides a preparation method of the novel compound feed for the leuciscus waleckii, and the obtained feed is used for the leuciscus waleckii and can promote the growth performance, the digestive function, the protein metabolism function, the lipid metabolism function, the immune function and the oxidation resistance of the leuciscus waleckii. The invention provides a theoretical reference for developing a novel non-grain protein source, saving fish meal protein, reducing the feed cost and optimizing the artificial compound feed for the leuciscus waleckii.

The invention relates to the technical field of treatment of thallus mash, in particular to a treatment method and system of thallus protein mash and application of the treatment method and system. The treatment method comprises the following steps: carrying out vacuum concentration on mycoprotein mash to obtain concentrated protein mash; carrying out wall breaking treatment on the concentrated protein mash to obtain concentrated protein mash containing thallus fragments; carrying out neutralization reaction on the concentrated protein mash by using an alkaline solution to obtain a concentrated protein reaction solution; sequentially carrying out enzymolysis reaction and enzyme deactivation treatment on the concentrated protein reaction solution by using a compound enzyme system to obtain a proteolysis reaction solution; wherein the compound enzyme system comprises the following raw materials: lysozyme, alkaline protease, nuclease and flavourzyme; and carrying out purification treatment on the proteolysis reaction liquid to obtain the mycoprotein treatment liquid. According to the treatment method, operations such as vacuum treatment, wall breaking treatment, neutralization reaction treatment and enzymolysis reaction are combined, and the content of peptide substances in the mycoprotein mash is effectively increased to 25% or above.

The invention relates to the field of feed raw materials, and discloses a feed plant enzymolysis protein with a strong food calling effect and a preparation method thereof, the preparation method comprises the following steps: S1, dispersing and reducing viscosity: reducing the viscosity of a protein powder raw material through shearing force generated by high-speed rotation of a dispersing disc; step S2, enzymolysis of polysaccharide substances: plant protein powder is added into a reaction kettle in which water is added in advance, then polysaccharide enzymes are added, and enzymolysis is performed; step S3: proteolysis: adding sodium carbonate to adjust pH, adding compound protease into the reaction kettle, and performing enzymolysis; and S4, conveying the wet material to a spray drying tower for spray drying. According to the method, the pea powder syrup protein powder, the wheat protein powder and the soybean concentrated protein powder are used as raw materials, through dispersion, enzymolysis and spray drying, flavor substances are generated, the bitter taste is eliminated, the palatability of plant protein is remarkably improved, the strong food calling performance is shown in feeding experiments of piglets and cats, and the method is few in steps, mild in reaction condition and low in cost.

As a series of viscosity-reducing and stabilizing formulations for high-concentration protein formulations, they are finely tuned to significantly reduce the viscosity of high-concentration biopharmaceuticals and increase their stability. Viscosity-reducing and stabilizing formulations can be used for high-concentration biopharmaceutical products and have significant potential to improve stability during manufacturing and storage, thereby increasing the feasibility of high-concentration biopharmaceutical products.

The invention relates to the field of cereal food processing, in particular to a method for enhancing the film-forming property of enzymolysis cereal food produced through roller drying, and the enzymolysis cereal food is prepared from the following raw materials in parts by weight: 500-1000 parts of cereals and 0-150 parts of protein concentrate. 0-270 parts of edible vegetable oil and 10-100 parts of non-starch polysaccharide. Comprising two processes of performing enzymolysis on non-high-protein cereal food and performing enzymolysis on high-protein cereals. The preparation method of the enzymolysis non-high-protein cereal film comprises the following steps: grinding cereals into powder and mixing pulp; performing enzymolysis; preparing materials: adding 10-100 parts of non-starch polysaccharide into the enzymolysis slurry; roller drying and enzyme deactivation; 2, performing enzymolysis on the high-protein cereal to form a film, namely performing enzymolysis on protein, grinding the cereal into powder, performing size mixing and performing enzymolysis; preparing materials; combining the enzymolysis protein slurry and the enzymolysis grain slurry; and roller drying and enzyme deactivation. The non-starch polysaccharide is one or a mixture of more of caesalpinia spinosa gum, guar gum, propylene glycol alginate, calcium alginate, potassium alginate, sodium alginate, locust bean gum, xanthan gum, tamarind polysaccharide gum and the like.

The invention discloses a method for preparing protein concentrate from high-denaturation oilseed meal, and belongs to the technical field of edible protein processing. The method comprises the following steps: crushing and screening to obtain meal powder with different protein contents by utilizing the distribution rule of proteins and structural carbohydrates in the microstructure of the high-denaturation oilseed meal and the physical property characteristics of components, and carrying out graded processing treatment according to the property difference of the meal powder; an air-electric field separation device is used for treating large-particle oilseed meal, high-fiber components which are difficult to remove in a traditional process are separated out, and high-protein components with solubility (NSI) similar to that of low-denaturation soybean meal and high gel property are obtained; proper concentration or alcoholase treatment is selected according to the protein content, denaturation degree and structural characteristics of the screening component, and a concentrated protein product meeting specific protein content requirements and / or functional characteristics can be obtained.

The invention provides an electrode for detecting protein substances, a preparation method of the electrode, a biosensor and a microflow detector. The electrode comprises a carbon electrode, a Prussian blue layer and a gold nanoparticle layer which are stacked in sequence, the side, away from the Prussian blue layer, of the gold nanoparticle layer is modified with an aptamer, the particle size of gold nanoparticles of the gold nanoparticle layer ranges from 20 nm to 100 nm, and the mass of the gold nanoparticles on the Prussian blue layer per unit area ranges from 4 micrograms / cm < 2 > to 5 micrograms / cm < 2 >; the Prussian blue layer is formed on one side of the carbon electrode by adopting a cyclic voltammetry method, the cyclic voltammetry method adopts a three-electrode system to perform continuous scanning for 20-40 circles in a potential range of-0.2 V-0. 9V, an electrolyte comprises 5-25 mM of potassium ferricyanide, 5-25 mM of ferric chloride, 0.12-0.25 M of hydrochloric acid, 5-50 mM of potassium chloride and the balance of water, and the molar ratio of the potassium ferricyanide to the ferric chloride is 1: 1. The electrode has more excellent sensitivity and stability, and detection of pg / mL-magnitude ultralow-concentration protein substances is realized.

Disclosed are an excipient composition for reducing the viscosity of a protein drug and a low-viscosity high-concentration protein composition preparation comprising the same, which have very low viscosity at a high protein concentration of about 20% or more, thereby being easily prepared, stored and used, and having a low protein drug viscosity and a low-viscosity high-concentration protein composition preparation comprising the same. And the method is particularly suitable for preparing injections for antibody therapy for subcutaneous and intramuscular administration. In particular, the high-concentration protein preparation can maintain a low viscosity even when stored at a low temperature, and thus can be used immediately after storage without separate preheating.

The application discloses a plant immunity elicitor protein PpAE4 and application thereof, the protein can induce plant resistance, and can be applied to the field of plant disease prevention and treatment as a plant immunity activator. The amino acid sequence of the protein is shown as SEQ ID NO:2. High-concentration protein can be obtained by using an engineering strain for expression, and the protein can be used for treating plants to improve the disease resistance of the plants to soybean rust fungi, and the required concentration is low, the effect is quick, and the action time is long. The PpAE4 provides a new way for improving the disease resistance of plants and preventing and treating plant diseases.

The invention provides a solvent dosage accurate regulation and control method for preparing dephenolized cottonseed protein concentrate, which comprises the following steps: shelling, crushing and determining indexes of a cottonseed sample to obtain stable spectrum data and basic label data, and selecting southern and northern Xinjiang mixed cottonseeds for difference analysis to obtain basic processing frequency parameters; based on basic processing frequency parameters, converting total solvent dosage and content rules, preparing dephenolized concentrated cottonseed protein, and performing quality analysis to obtain a quality evaluation result; carrying out correction, exception processing, preprocessing, standardization and data set division on the stable spectral data to obtain a modeling data set; and constructing a hyperspectral quantitative correction model to obtain the predicted solvent dosage, and carrying out cross validation on the hyperspectral quantitative correction model. According to the method, the consumption of the reagent in the extraction process of preparing the dephenolized cottonseed protein concentrate is dynamically regulated and controlled through the model, so that the accurate estimation and regulation and control of the reagent consumption in the treatment process of different varieties of cottonseeds are realized.

The invention relates to diagnostic methods, and particularly, although not exclusively, to the use of oligomeric protein traps in sample diagnostics. The invention extends to the use of oligomeric protein traps as binding agents, for example in methods for enriching samples of low-concentration proteins and / or genetic material, and methods for detecting the same in samples. The invention also encompasses high-affinity protein traps and their application in environmental monitoring, diagnosis and therapy.

The invention discloses a soybean protein concentrate milk beverage and a preparation method thereof, and belongs to the field of food processing. According to the invention, the problem of low solubility of the soybean protein concentrate at present is solved, and the application of the soybean protein concentrate in the beverage market is expanded. The preparation method of the soybean protein concentrate milk beverage comprises the following steps: modifying soybean protein concentrate powder by using an ultrasonic technology to improve the solubility of soybean protein concentrate, then adding xanthan gum to improve the emulsibility and stability of soybean protein concentrate milk, and mixing the soybean protein concentrate milk with ingredients through high-pressure homogenization to obtain the soybean protein concentrate milk beverage. According to the method, the application range of the soybean protein concentrate is expanded, and the soybean protein concentrate can be widely applied to the field of food processing.

The invention discloses abalone feed with gulfweed powder partially replacing kelp powder and a preparation method of the abalone feed, and belongs to the technical field of cultivation. The abalone feed is prepared from the following components in percentage by weight based on the total weight of the feed: 10% of gulfweed powder, 20% of kelp powder, 20% of high gluten flour, 20% of soybean meal, 20% of shell powder, 20% of fish meal, 10% of soybean protein concentrate, 10% of flour, 10% of monocalcium phosphate, 20% of choline chloride, 10% of fish oil, 10% of soybean oil, 10% of vitamin premix and 10% of mineral premix. By reasonably designing the proportion of the gulfweed powder and the kelp powder in the abalone feed formula, the feed preparation cost and abalone feeding cost can be effectively saved, meanwhile, the abalone feed has no significant influence on abalone growth, and healthy and sustainable development of marine fishery resources is facilitated.

Provided herein are methods for producing a mung bean protein isolate having high functionality for a broad range of food applications. In some embodiments, the methods for producing the isolate comprise one or more steps selected from: (a) extracting one or more mung bean proteins from a mung bean protein source in an aqueous solution, for example, at a pH between about 6.5-10.0; (b) purifying protein from the extract using at least one of two methods: (i) precipitating protein from the extract at a pH near the isoelectric point of a globulin-rich fraction, for example a pH between about 5.0-6.0; and / or (ii) fractionating and concentrating protein from the extract using filtration such as microfiltration, ultrafiltration or ion-exchange chromatography; and (c) recovering purified protein isolate.

The application provides a SERS method for detecting protein based on a sandwich method combining gold nanoparticle probes and antibodies, wherein the method can realize high sensitivity and high selectivity by functionalizing specific antibodies on the surface of gold nanoparticles and combining a sandwich detection strategy, is suitable for detecting ultra-low concentration protein, and has the potential to be expanded to multi-marker detection, and by selecting Raman reporter molecules with characteristic peak separation, signal overlap and background interference can be effectively reduced.

The invention provides walnut concentrated protein powder. The walnut concentrated protein powder is prepared by adopting the following method: (1) squeezing walnut kernels with seed coats at low temperature to prepare oil; (2) crushing the walnut cake meal, and degreasing through a subcritical extraction technology, so that the residual oil rate of the cake meal is reduced to 1-3%; (3) preliminarily removing seed coats in the walnut cake meal; and 4) finely separating the seed coat fragments from the walnut concentrated protein powder. The method provided by the invention adopts a normal-temperature dry method for preparation, realizes efficient and sufficient separation of walnut kernel seed coats and proteins in a powder state, obtains the walnut concentrated protein powder with the protein content of more than 70%, has the advantages of high efficiency, no pollution, water saving, energy saving and the like, and has a wide application prospect.

The invention discloses a production process of high-purity low-fat animal protein powder, which comprises the following steps: S1, selecting animal fat, and carrying out pretreatment of impurity removal and cleaning; s2, the pretreated raw materials are put into closed cooking equipment for high-temperature and high-pressure cooking, and oil-water protein three-phase layering occurs; s3, collecting a middle-layer protein liquid phase, and performing refined filtration to obtain refined protein liquid; s4, performing vacuum concentration on the refined protein liquid to obtain concentrated protein liquid; and S5, carrying out spray drying on the concentrated protein liquid to prepare an animal protein powder finished product, wherein the protein content of the finished product is greater than or equal to 90%. Compared with the prior art, the production process has the advantages that a production method based on combination of high-temperature cooking vaporization, physical layering and spray drying is provided, and the production process of the high-purity low-fat animal protein powder with the protein content larger than or equal to 90% is achieved.

The invention relates to a method and a device for enhancing immunoblotting and immunoblotting electrophoresis sensitivity. The method comprises the following steps: performing regular stripe standing wave concentration on protein by utilizing regular stripe standing waves generated by superposing surface sound source ultrasonic waves through a gel electrophoresis carrier which is subjected to electrophoretic separation and contains a low-concentration protein molecule band in a direction vertical to electrophoretic separation; and the protein molecules with different densities are pushed to the node or antinode region of the standing wave and are concentrated. The device comprises an area sound source generator operated by the method and an echo carrier plate with an echo plate. An electrophoresis tank for accommodating an echo carrying disc is additionally arranged, so that the electrophoretic separation step and the concentration step are integrated; and a main bearing body is arranged for bearing the electrophoresis tank, a related power supply and a related switch. According to the method and the device, the concentration of the low-abundance protein in the reaction area reaches or is closer to the optimal concentration for antigen-antibody reaction, in the immunoblotting process, the local luminescence or fluorescence peak intensity after membrane transfer meets the condition of reaching a threshold value or above, and the detection rate of the low-abundance target protein is greatly improved.

The present invention provides a single-molecule detection and analysis method for detecting low-abundance biomarker proteins based on proximity ligation, which solves the technical problems of low sensitivity and low precision of existing real-time fluorescence quantitative PCR detection methods based on PLA. The present invention divides the reaction system into a plurality of micro-droplet systems, completes QPCR or PCR cyclic amplification reactions inside the micro-droplets, realizes signal amplification, and thus accurately quantifies the protein concentration. The present invention evenly distributes the reaction system in a plurality of droplet systems, performs signal amplification on them through nucleic acid amplification technology, thereby improving the signal detection rate of low-concentration proteins in the droplets, and can perform multiple protein detection quantification.

The invention belongs to the technical field of soybean processing, and particularly relates to a soybean protein concentrate processing technology which comprises the following steps: step 1, raw material pretreatment and acid precipitation; step 2, dehydration treatment; step 3, drying treatment; step 4, dedusting and conveying; step 5, packaging; raw material pretreatment and acid precipitation are as follows: low-temperature squeezed soybean cake powder is put into a stirring tank, treated pure hot water with the temperature being 50-55 DEG C and the volume being 7 times that of the materials is added into the tank, meanwhile, citric acid is added and stirred until the materials are fully dissolved, and the materials are soaked and stirred for 40-45 minutes, so that water-soluble components in the materials are fully dissolved. The method solves the problems of low raw material utilization rate, difficulty in product activity control and byproduct waste in the traditional process, realizes efficient preparation of the soybean protein concentrate and recycling of byproducts, and improves production benefits and product quality.

The present disclosure relates to formulations comprising a protein having an Fc region (e.g., an antibody) and a surfactant such as polysorbate (e.g., polysorbate 20), and methods of purifying the protein that improve the stability of the surfactant such as polysorbate in the formulation.

The present invention relates to hydrogels that are capable of binding to proteins, and additionally concentrating, labelling and releasing said labelled and concentrated proteins, are provided. Corresponding formulations, oral formulations, biomaterials, uses, methods and kits of parts are also provided.

The utility model provides an ultrafiltration concentration tube device with a sterile filtration function, which solves the problems that the existing ultrafiltration tubes with different specifications and models can only achieve the purpose of protein concentration, different proteins can be confused due to frequent operation after the concentration is finished, the recovery rate is reduced, the risk of introducing bacteria is increased and the like. According to the main scheme, a tube body internally provided with a cavity is included, the top of the tube body is open, the tube body can be sealed into a whole through a tube cover, the tube body is formed by splicing three sections, the adjacent splicing positions are fixed through deformation friction, and the three sections of the tube body sequentially comprise an antibody storage area, a liquid storage area and a liquid discharging area from the upper end opening to the lower end opening; a first supporting table and a second supporting table are fixed to the two sequentially-connected positions of the inner wall of the pipe body respectively, a sterile filter membrane is embedded in the first supporting table, and an ultrafiltration membrane is embedded in the second supporting table.

The application relates to a compound soybean concentrated protein, which comprises the following components: 50-72 parts of soybean protein isolate, 4-13 parts of soybean dietary fiber, 20-25 parts of soybean powder, 0.5-0.8 parts of ethyl maltol, 21-27 parts of water-soluble coating taste-improving particles, and 2-3 parts of an emulsifier, wherein the water-soluble coating taste-improving particles comprise a coating wall mixture and a core mixture, and the mass ratio of the coating wall mixture to the core mixture is 5:1. The beneficial effects are as follows: compared with a product made of traditional compound concentrated protein, the product has significant improvement in elasticity and hardness, the mixing time with sausage meat is shortened, the mixing production efficiency is improved, the product has the advantages of being delicate and glossy, not sticking to sausage casing, lubricated taste, high hardness, good elasticity, no water and oil seepage phenomenon, high yield, good water and oil retention effect, and the like, the edible spices are stored in a closed manner, and are mixed into the sausage at last during mixing and stirring, the sausage has more sufficient fragrance, the yield is improved, and the taste of the product is improved.

The invention provides an analysis method for accurately measuring the concentration of high-concentration protein, which comprises the following steps: adopting an electric pipette, optimizing the specification, power, gun head rinsing times and the like of the pipette, limiting the standing time of a sample, eliminating a bubble method and determining operation conditions such as proper sample detection quantity, detection wavelength, protein concentration range and the like. The technical problem that high-concentration protein is difficult to accurately transfer and measure the concentration due to high viscosity is solved. The method can effectively reduce the measurement error, improve the detection precision and stability, ensure that the concentration detection result of the high-concentration protein is extremely small in relative standard deviation and high in accuracy, provide reliable technical guarantee for research and development and industrial production of high-concentration protein preparations, and powerfully promote the development of related research and production in the biotechnology field.