36 results about "Concentration protein" patented technology

As a series of viscosity-reducing and stabilizing formulations for high-concentration protein formulations, they are finely tuned to significantly reduce the viscosity of high-concentration biopharmaceuticals and increase their stability. Viscosity-reducing and stabilizing formulations can be used for high-concentration biopharmaceutical products and have significant potential to improve stability during manufacturing and storage, thereby increasing the feasibility of high-concentration biopharmaceutical products.

The application discloses a plant immunity elicitor protein PpAE4 and application thereof, the protein can induce plant resistance, and can be applied to the field of plant disease prevention and treatment as a plant immunity activator. The amino acid sequence of the protein is shown as SEQ ID NO:2. High-concentration protein can be obtained by using an engineering strain for expression, and the protein can be used for treating plants to improve the disease resistance of the plants to soybean rust fungi, and the required concentration is low, the effect is quick, and the action time is long. The PpAE4 provides a new way for improving the disease resistance of plants and preventing and treating plant diseases.

The invention provides a solvent dosage accurate regulation and control method for preparing dephenolized cottonseed protein concentrate, which comprises the following steps: shelling, crushing and determining indexes of a cottonseed sample to obtain stable spectrum data and basic label data, and selecting southern and northern Xinjiang mixed cottonseeds for difference analysis to obtain basic processing frequency parameters; based on basic processing frequency parameters, converting total solvent dosage and content rules, preparing dephenolized concentrated cottonseed protein, and performing quality analysis to obtain a quality evaluation result; carrying out correction, exception processing, preprocessing, standardization and data set division on the stable spectral data to obtain a modeling data set; and constructing a hyperspectral quantitative correction model to obtain the predicted solvent dosage, and carrying out cross validation on the hyperspectral quantitative correction model. According to the method, the consumption of the reagent in the extraction process of preparing the dephenolized cottonseed protein concentrate is dynamically regulated and controlled through the model, so that the accurate estimation and regulation and control of the reagent consumption in the treatment process of different varieties of cottonseeds are realized.

The invention relates to diagnostic methods, and particularly, although not exclusively, to the use of oligomeric protein traps in sample diagnostics. The invention extends to the use of oligomeric protein traps as binding agents, for example in methods for enriching samples of low-concentration proteins and / or genetic material, and methods for detecting the same in samples. The invention also encompasses high-affinity protein traps and their application in environmental monitoring, diagnosis and therapy.

The invention discloses abalone feed with gulfweed powder partially replacing kelp powder and a preparation method of the abalone feed, and belongs to the technical field of cultivation. The abalone feed is prepared from the following components in percentage by weight based on the total weight of the feed: 10% of gulfweed powder, 20% of kelp powder, 20% of high gluten flour, 20% of soybean meal, 20% of shell powder, 20% of fish meal, 10% of soybean protein concentrate, 10% of flour, 10% of monocalcium phosphate, 20% of choline chloride, 10% of fish oil, 10% of soybean oil, 10% of vitamin premix and 10% of mineral premix. By reasonably designing the proportion of the gulfweed powder and the kelp powder in the abalone feed formula, the feed preparation cost and abalone feeding cost can be effectively saved, meanwhile, the abalone feed has no significant influence on abalone growth, and healthy and sustainable development of marine fishery resources is facilitated.

Provided herein are methods for producing a mung bean protein isolate having high functionality for a broad range of food applications. In some embodiments, the methods for producing the isolate comprise one or more steps selected from: (a) extracting one or more mung bean proteins from a mung bean protein source in an aqueous solution, for example, at a pH between about 6.5-10.0; (b) purifying protein from the extract using at least one of two methods: (i) precipitating protein from the extract at a pH near the isoelectric point of a globulin-rich fraction, for example a pH between about 5.0-6.0; and / or (ii) fractionating and concentrating protein from the extract using filtration such as microfiltration, ultrafiltration or ion-exchange chromatography; and (c) recovering purified protein isolate.

The application provides a SERS method for detecting protein based on a sandwich method combining gold nanoparticle probes and antibodies, wherein the method can realize high sensitivity and high selectivity by functionalizing specific antibodies on the surface of gold nanoparticles and combining a sandwich detection strategy, is suitable for detecting ultra-low concentration protein, and has the potential to be expanded to multi-marker detection, and by selecting Raman reporter molecules with characteristic peak separation, signal overlap and background interference can be effectively reduced.

The invention discloses a production process of high-purity low-fat animal protein powder, which comprises the following steps: S1, selecting animal fat, and carrying out pretreatment of impurity removal and cleaning; s2, the pretreated raw materials are put into closed cooking equipment for high-temperature and high-pressure cooking, and oil-water protein three-phase layering occurs; s3, collecting a middle-layer protein liquid phase, and performing refined filtration to obtain refined protein liquid; s4, performing vacuum concentration on the refined protein liquid to obtain concentrated protein liquid; and S5, carrying out spray drying on the concentrated protein liquid to prepare an animal protein powder finished product, wherein the protein content of the finished product is greater than or equal to 90%. Compared with the prior art, the production process has the advantages that a production method based on combination of high-temperature cooking vaporization, physical layering and spray drying is provided, and the production process of the high-purity low-fat animal protein powder with the protein content larger than or equal to 90% is achieved.

The invention relates to a method and a device for enhancing immunoblotting and immunoblotting electrophoresis sensitivity. The method comprises the following steps: performing regular stripe standing wave concentration on protein by utilizing regular stripe standing waves generated by superposing surface sound source ultrasonic waves through a gel electrophoresis carrier which is subjected to electrophoretic separation and contains a low-concentration protein molecule band in a direction vertical to electrophoretic separation; and the protein molecules with different densities are pushed to the node or antinode region of the standing wave and are concentrated. The device comprises an area sound source generator operated by the method and an echo carrier plate with an echo plate. An electrophoresis tank for accommodating an echo carrying disc is additionally arranged, so that the electrophoretic separation step and the concentration step are integrated; and a main bearing body is arranged for bearing the electrophoresis tank, a related power supply and a related switch. According to the method and the device, the concentration of the low-abundance protein in the reaction area reaches or is closer to the optimal concentration for antigen-antibody reaction, in the immunoblotting process, the local luminescence or fluorescence peak intensity after membrane transfer meets the condition of reaching a threshold value or above, and the detection rate of the low-abundance target protein is greatly improved.

The invention belongs to the technical field of soybean processing, and particularly relates to a soybean protein concentrate processing technology which comprises the following steps: step 1, raw material pretreatment and acid precipitation; step 2, dehydration treatment; step 3, drying treatment; step 4, dedusting and conveying; step 5, packaging; raw material pretreatment and acid precipitation are as follows: low-temperature squeezed soybean cake powder is put into a stirring tank, treated pure hot water with the temperature being 50-55 DEG C and the volume being 7 times that of the materials is added into the tank, meanwhile, citric acid is added and stirred until the materials are fully dissolved, and the materials are soaked and stirred for 40-45 minutes, so that water-soluble components in the materials are fully dissolved. The method solves the problems of low raw material utilization rate, difficulty in product activity control and byproduct waste in the traditional process, realizes efficient preparation of the soybean protein concentrate and recycling of byproducts, and improves production benefits and product quality.

The application relates to a compound soybean concentrated protein, which comprises the following components: 50-72 parts of soybean protein isolate, 4-13 parts of soybean dietary fiber, 20-25 parts of soybean powder, 0.5-0.8 parts of ethyl maltol, 21-27 parts of water-soluble coating taste-improving particles, and 2-3 parts of an emulsifier, wherein the water-soluble coating taste-improving particles comprise a coating wall mixture and a core mixture, and the mass ratio of the coating wall mixture to the core mixture is 5:1. The beneficial effects are as follows: compared with a product made of traditional compound concentrated protein, the product has significant improvement in elasticity and hardness, the mixing time with sausage meat is shortened, the mixing production efficiency is improved, the product has the advantages of being delicate and glossy, not sticking to sausage casing, lubricated taste, high hardness, good elasticity, no water and oil seepage phenomenon, high yield, good water and oil retention effect, and the like, the edible spices are stored in a closed manner, and are mixed into the sausage at last during mixing and stirring, the sausage has more sufficient fragrance, the yield is improved, and the taste of the product is improved.

The invention discloses a solid-phase extraction-liquid chromatography detection method for organic acid salts in assisted reproduction liquid, and relates to the field of in-vitro assisted reproduction liquid detection, the method comprises the following steps: (1) dissolving and diluting the organic acid salts with a mobile phase respectively to obtain a reference substance stock solution; (2) adding an eluent into the reference substance stock solution, carrying out nitrogen blowing concentration, and fixing the volume by using a mobile phase to obtain a reference substance solution; (3) mixing the assisted reproduction liquid and the treating agent, and incubating to obtain a pretreated test sample; performing solid-phase extraction on the pretreated test sample to obtain a test sample solution; and (4) injecting the blank solution, the test solution and the reference solution into a high performance liquid chromatograph, and testing under a chromatographic condition. According to the method, pretreatment and solid-phase extraction technologies are combined, so that the matrix effect of impurities such as high-concentration protein in detection of organic acid salts in the assisted reproduction liquid is reduced, instruments are protected, the selectivity of the detection method is improved, and the accuracy of qualitative and quantitative detection is improved.

The present application relates to the technical field of feed processing, and discloses a kind of high-protein straw compound feed and preparation method thereof, solve straw burning pollution, and the technical problem of low breeding efficiency and single nutrition of pure straw feed.The present application uses agricultural straw as main material, and innovatively introduces high-concentration protein mother liquor produced by grain deep processing enterprises as core nutritional supplement.The mother liquor is rich in natural protein, amino acids, minerals, vitamins and other core nutrients required for animal growth, which can effectively replace part of artificial feed additives and has the effect of promoting animal growth.The feed prepared by the present application can ensure that the breeding efficiency is not lower than or slightly lower than that of pure grain feed, and can minimize the harmful component residues in the animal body;At the same time, it realizes the efficient resource utilization of agricultural straw, reduces straw burning pollution from the root, reduces feed production cost, realizes the reutilization of grain deep processing byproducts, and can be widely used in various livestock and poultry breeding.

The invention particularly relates to a composite functional pet phagostimulant and a preparation method thereof.The preparation method comprises the following steps that 1, after chicken liver pulp, chicken oil and concentrated protein liquid are subjected to enzymolysis, zymolyte is collected; (2) carrying out Maillard reaction on the zymolyte obtained in the step (1), yeast hydrolysate and reducing sugar step by step; (3) after cooling in the step (2), adding a nutrition enhancer, an intestinal health agent, an antioxidant and a stabilizer, and homogenizing and stabilizing; and (4) adding an acidity regulator and a preservative into the mixture obtained in the step (3), uniformly mixing, filling, sterilizing and packaging. The raw material of the added concentrated protein liquid is cooking residual water of meat, comprehensive utilization can reduce the pressure of wastewater treatment, meanwhile, more nutrient substances are added to the phagostimulant, functional additives in the phagostimulant are stably embedded, the antioxidant slow-release effect and the intestinal tract targeted release effect of prebiotics are enhanced, and the nutritional value of the phagostimulant is improved. The added value of the phagostimulant is improved, and the phagostimulant has a good commercial application prospect.

A process for producing a fermentation product and a concentrated protein co-product from a starch-containing material.

The invention discloses a crucian feed with soybean meal replaced by cottonseed protein concentrate and application of the crucian feed. Cottonseed protein concentrate is used as a main plant protein source and completely replaces soybean meal in a feed formula, and the mass percent of the cottonseed protein concentrate is 19.78%. The feed further comprises the following components: fish meal, casein, flour, fish oil, soybean oil, vitamins, mineral premix and the like. The feed provided by the invention can significantly improve the ingestion speed and the ingestion amount of crucian carps and effectively promote the growth of crucian carps, and the weight gain rate, the specific growth rate and the feed efficiency of the feed are significantly superior to those of a feed containing soybean meal. The feed can regulate and control lipid metabolism related gene expression of crucian muscle and liver, enhance fat synthesis and catabolism, promote muscle protein and fat deposition and increase muscle polyunsaturated fatty acid content, and has no negative influence on liver health and intestinal flora homeostasis. The invention provides an efficient solution for soybean meal decrement substitution, and has important application value.

A process for producing a fermentation product and a concentrated protein co-product from a starch-containing material e.g. by (a) liquefying a starch-containing material at a temperature above the initial gelatinization temperature of the starch with an alpha-amylase to produce a liquefied mash; (b) subjecting the liquefied mash to a protein concentration process to produce a concentrated protein co-product, a soluble starch stream and a sugar stream; (c) saccharifying the soluble starch stream and the sugar stream with a glucoamylase to produce a fermentable sugar; (d) fermenting the sugar with a fermenting organism to produce the fermentation product, wherein at least one separation step is performed after the liquefaction in step (a) resulting in a liquid portion comprising soluble starch / oligosacchrides and an insoluble portion comprising solids from the liquefied mash, said insoluble portion undergoing said protein concentration process.

The invention discloses a special emulsifier for a high-protein cake and a preparation method thereof, and belongs to the field of food additives. Aiming at potential safety hazards and nutritional defects of a traditional chemical synthetic emulsifier, a novel plant-based emulsification system is constructed by compounding soybean protein isolate, soybean protein concentrate, starch, dietary fibers and a functional thickening agent. According to the emulsifying agent, 40-60 parts of soybean protein isolate is used as a core emulsifying component, 5-15 parts of soybean protein concentrate is supplemented to enhance the water binding capacity, 10-30 parts of starch is combined to regulate and control the rheological property of paste, and 2-10 parts of soybean dietary fibers and 1-6 parts of a thickening agent are added to form a synergistic network. The emulsifier disclosed by the invention can obviously improve the stability of cake batter (bubble height difference is less than or equal to 4mm), increase the specific volume of a finished product (4.95-5.25 ml / g), and endow the product with high protein content (greater than or equal to 15g / 100g) and natural nutritional characteristics. The traditional chemical emulsifier can be completely replaced, and the requirements of the healthy baking industry for safe, nutritional and functional products are met.

This invention discloses a protein solution concentration device, comprising: a concentration mechanism; a pressure mechanism; and fluid pipelines. The concentration mechanism includes: a first housing and a second housing, the first housing having an inlet for the protein solution to enter the concentration mechanism and a first outlet for the concentrated solution to exit the concentration mechanism; the second housing having a second outlet for waste liquid to exit the concentration mechanism; and an ultrafiltration membrane assembly disposed within the first and second housings. One side of the ultrafiltration membrane assembly has a first flow channel connecting the inlet to the outlet, and the other side of the ultrafiltration membrane assembly has a second flow channel connecting to the second outlet. With the above configuration, the concentration mechanism has a simple and practical structure, enabling rapid and efficient protein concentration while ensuring that the protein's activity and structure are not damaged. Simultaneously, the ultrafiltration membrane assembly of this invention exhibits low protein adhesion and high flux, avoiding waste due to protein adhesion during concentration.

The invention provides a preparation method of ultra-high-pressure sterilized sheep yoghourt with a long shelf life. The preparation method comprises the following steps: S1) carrying out ultra-high-pressure treatment on concentrated protein powder; s2) dissolving, hydrating, degassing and homogenizing the concentrated protein powder subjected to ultrahigh pressure treatment and raw goat milk to obtain a mixed raw material; s3) performing first ultrahigh-pressure sterilization treatment on the mixed raw materials to obtain sterilized raw materials; s4) adding a strain into the sterilized raw material for fermentation to obtain a fermented material; and S5) performing secondary ultrahigh-pressure sterilization treatment on the fermented material to obtain the ultrahigh-pressure sterilized long-shelf-life sheep yogurt. Compared with the prior art, a two-stage sterilization mode is adopted, raw goat milk can be treated, needed auxiliary materials and goat yoghurt can be processed, and therefore the sensory property and the shelf life of the goat yoghurt are improved, and the sensory property and the shelf life of the ultra-high pressure goat yoghurt are better than those of heat sterilization normal-temperature goat yoghurt.

The invention relates to the technical field of feed processing, and discloses a compound growth-promoting feed for breeding beef cattle and sheep. The feed additive is prepared from the following raw materials: protoparanase activity, cellulase activity, beta-glucanase activity, protease activity, bacillus subtilis, lactobacillus plantarum, high-activity saccharomycetes, biological cell fission, enveloped compound vitamins, compound amino acids, sodium butyrate, sodium humate, guanidinoacetic acid, amino acid chelated iron, copper, zinc, disease-resistant factors and health-care factors. The feed is prepared from bioactive small peptides, lysozyme, TA-85, soybean protein concentrate, peanut protein, whey protein powder, bee pollen, yolk powder, fish meal and the like. According to the compound growth-promoting feed for beef cattle and sheep breeding as well as the preparation method and the application of the compound growth-promoting feed, the compound growth-promoting feed is based on rumen digestion characteristics of beef cattle and sheep, and is scientifically matched with an enzyme preparation, a multi-element protein raw material and nutrient enrichment components in an accurate ratio, so that the compound growth-promoting feed can fully meet nutritional requirements of beef cattle and sheep in different growth stages.

The present disclosure relates, according to some embodiments, to methods and systems for processing a high-concentration protein product from a microcrop (e.g., aquatic species, Lemna) and compositions thereof. According to some embodiments, the present disclosure relates to a method of processing a biomass comprising a microcrop (e.g., Lemna), where the method may include: blanching a first portion of the biomass in a blanching solution to form a wet protein concentrate; separating the first wet protein concentrate from a separated solution (e.g., using a screw press, using a vibratory screen); and drying the first wet protein concentrate to form at least one of a first protein concentrate flake and a first protein concentrate granule. In some embodiments at least one of the first protein concentrate flake and the first protein concentrate granule may comprise at least 45% protein and a Protein Digestibility Corrected Amino Acid Score (PDCAAS) value of at least 0.88. In some embodiments, the present disclosure relates to protein products and compositions derived from a microcrop (e.g., derived from Lemna).

This invention provides a functional feed for improving the quality of farmed large yellow croaker, belonging to the field of feed technology. The formula of the functional feed provided by this invention includes the following components: fish meal, chicken meal, Clostridium ethanol, cottonseed protein concentrate, enzymatically hydrolyzed chicken syrup, tapioca flour, wheat flour, fish oil, soybean oil, soybean lecithin, vitamin premix, mineral premix, choline chloride, calcium dihydrogen phosphate, compound vitamin supplement, compound amino acid supplement, kelp powder containing gum, ethoxyquin, antifungal agent, antioxidant TBHQ, microcrystalline cellulose, guanidinoacetic acid, 5-aminoacetic acid propionic acid, and Eucommia ulmoides leaf extract. This invention, through the combination of different formulas and components, produces a synergistic effect in reducing drip loss and cooking loss in large yellow croaker muscle, further enhancing the role of functional feed in improving quality.

The application belongs to the technical field of nano drugs, and particularly relates to a protein cage nano drug with a slow-release effect and a preparation method and application thereof. The protein cage nano drug with the slow-release effect is provided in the application, a protein binding peptide is modified at a C end of a protein subunit monomer of a nano protein cage, and is combined with a protein with a long half-life in a body, so that a drug action time is prolonged, a drug efficacy is improved, and a possible drug toxic side effect is weakened. Meanwhile, the protein binding peptide is selected from a serum albumin binding peptide with an amino acid sequence as shown in SEQ ID NO. 1, so that the protein cage nano drug provided in the technical solution can be combined with the most abundant endogenous protein in blood, and the half-life of the high-concentration protein cage nano drug can also be prolonged. The technical problem that there is a lack of slow-release protein cage nano drugs and it is difficult to release drugs for a long time in the prior art is solved.

The invention discloses a method for efficiently extracting cannabis sativa seed protein concentrate. The method comprises the following steps: S1, shelling cannabis sativa seeds; s2, squeezing the cannabis sativa seeds at low temperature or pressing the cannabis sativa seeds into blank pieces, and then performing low-temperature subcritical extraction; s3, performing low-temperature subcritical extraction; s4, crushing: crushing the meal obtained in the step S4 into 40-120 meshes; s5, acid precipitation; s6, separating the sediment; s7, adjusting the pH value of the sediment to be 7, and controlling the material temperature to be 15-65 DEG C in the adjusting process; s8, evaporating and drying to obtain cannabis sativa seed protein concentrate; in the steps, no other steps of dissolving and extracting protein are needed before acid precipitation. According to the method, the extraction rate of the protein is greatly improved.

The invention discloses a special high-concentration lysate for exosome protein extraction, and belongs to the technical field of exosome lysate. The reagent is mainly prepared by mixing a reagent SDS (Sodium Dodecyl Sulfate), NP-40, sodium deoxycholate, TritonX-100 and NaCl. The specific preparation method comprises the following steps: taking 10ml of 50mM. Tris-HCl buffer solution, adding 1g. SDS, 1g. NP-40, 0.1 g of sodium deoxycholate, 100. Mu l. TritonX-100 and 0.58 g. NaCl, and fully blowing, beating and uniformly mixing. A subversive exosome protein extraction solution is provided by organically integrating the two functional modules of'mild splitting 'and'synchronous concentration' into a whole. According to the formula of the lysate, exosomes can be mildly and specifically lysed under the condition that the concentration of effective components is extremely high, and the integrity of key membrane proteins such as CD9, CD63 and CD81 is perfectly protected; meanwhile, due to the built-in concentration capacity, a high-concentration protein sample can be obtained without relying on tedious steps such as super-separation in the cracking process. The special high-concentration lysate for exosome protein extraction achieves the effect of improving the efficiency and yield of exosome protein extraction.

Methods of making meat substitute food products and meat substitute food products are provided. To improve texture, mouthfeel, and / or prevent or delay hardening of a protein matrix, a meat substitute food product is made using a high moisture protein texturization process using in an extruder under conditions resulting in a continuous protein fiber matrix structure containing a disruption, i) at least one protein matrix forming ingredient, such as an isolated protein or a concentrated protein, ii) a starch-containing grain selected from the group consisting of ii.a) steeped grains, ii.b) germinated grains, ii.c) malted grains, ii.d) sprouted grains, or ii.e) any combination of two, three, or four of these starch-containing grains as one of the ingredients, and iii) water or an aqueous liquid, the extrudate containing starch located at the disruption and not emulsified with the protein fiber matrix structure.

The application belongs to the field of bioinformatics, and relates to a protein salt sensitivity prediction method based on feature engineering and ensemble learning. First, by inputting known low, medium and high salt concentration protein sequence data and performing data cleaning and length standardization, the system automatically extracts ten key physical and chemical characteristics such as net charge density and charge asymmetry. Second, a multi-model learning framework is built using random forest, gradient boosting tree and support vector machine, the integrated weight is dynamically allocated based on the accuracy of each model on the validation set, and the final classification result is generated by using the weighted voting mechanism. Finally, the integrated model is quantitatively evaluated and saved, and then new protein sequences are predicted, and the prediction category, confidence and feature importance analysis are output. The application realizes high-precision and interpretable prediction of protein salt sensitivity, and provides condition optimization support for liquid-liquid phase separation research and drug delivery system.

The invention discloses a method for predicting protein interaction, which comprises the following steps of: determining interaction data among proteins in the presence of different types and concentrations of pharmaceutic adjuvants through experiments, specifically, permeation second Wirie coefficients, and constructing a data set covering the interaction relationship between the types and the concentrations of the adjuvants and the proteins; fitting the data of the data set to an xDLVO model to obtain corresponding auxiliary material and protein model parameters; and inputting the types and concentrations of the auxiliary materials in the to-be-predicted solution environment and the obtained model parameters into the xDLVO model, and outputting a prediction result of protein-protein interaction. According to the method, the type and concentration data of the pharmaceutic adjuvants are collected, the protein-protein interaction data are combined, the prediction model is constructed, the protein interaction and the influence of the adjuvants on the protein stability can be efficiently evaluated, an effective tool is provided for early screening of the adjuvants in preparation development, and the method is suitable for popularization and application. And the development of a stable high-concentration protein preparation is promoted.

The application realizes significant and stable improvement of soybean protein taste by screening 100~1000 Da specific molecular weight chitooligosaccharide and optimizing whole process parameters. The application determines the interaction mechanism of chitooligosaccharide and soybean protein, changes the protein secondary structure through hydrogen bond, electrostatic and hydrophobic interaction, remodels the protein aggregation state and competitively combines with phytic acid, and fundamentally solves the core taste defects of soybean protein such as roughness, astringency and beany taste. The soybean protein product prepared by the application has delicate and warm taste, and at the same time, the nutritional ingredients are maximally retained, which meets the natural and healthy consumption demand. The preparation process does not need special large equipment, has low cost and small energy consumption, is suitable for various raw materials such as soybean protein isolate and soybean protein concentrate, can prepare various form products such as powder and liquid, is widely applied in the fields of food processing, health product research and development, feed additive and the like, and has significant industrial application value and market prospect.