251 results about "Concentration protein" patented technology

The present invention discloses a method for extracting rice protein from rice. Said method includes the following steps: cleaning rice, soaking, removing sand, grinding to make pulp, concentrating and drying rice protein; also includes the steps of fine-grinding rice pulp, adopting dished separating machine to make multistage separation and making the protein pulp undergo the process of enzyme treatment. The described enzyme treatment is characterized by that in the multistage separated and combined rice protein pulp the following enzymes are successively added to make reaction: adding 0.05%-0.2% of amylase, temp. is 60-70 deg.C and time is 40-60 min; adding 0.05%-0.2% of cellulase, temp. is 40-50 deg.C and time is 2-3 hr; and adding 0.1%-1% of lipase, temp. is 40-60 deg.C and time is 2-3 hr. Said method can implement industrial production of rice protein.

A system, processes, and milk-based food products made from the system and processes, in which cream is separated from milk to produce an ultra-low fat milk product. The milk product is microfiltered to produce a retentate that is ready to drink and is high in protein and has no or substantially no fat. The permeate from the microfiltration process is ultrafiltered to produce a retentate that is high in protein with few other solids. The permeate may be used to provide protein fortification to other food and beverage products, and is especially useful in its liquid form for such fortification.

The invention discloses a method for continuously extracting and preparing concentrated protein, separated protein and proteolysis products from almond meal. The method comprises the following steps of: degreasing and debitterizing residual oil meal obtained after almond oil is mechanically and coldly pressed, and then extracting with a low-concentration NaCl phosphate buffer solution to obtain the concentrated protein; preparing almond separated protein from the concentrated protein solution by applying an ultrafiltration membrane technology; preparing a proteolysis-almond polypeptide mixed solution from the separated protein solution by applying an enzymolysis technology; and ultrafiltering the polypeptide mixed solution by applying a membrane technology to obtain multifunctional active almond peptide with different class level molecular weights in sequence. The method has the advantages of simple process, strong operability and easiness in control of extracting conditions and can finish the extraction and preparation of the almond concentrated protein, the separated protein and the proteolysis products in sequence; and the obtained products have high purity and wide application and can be directly used as food additives, nutrition reinforcers, health care products, medicaments, and the like needed by the protein foods and different processing foods.

The invention provides, among other things, lyophilized compositions of high surface area that comprise a protein and that reconstitute quickly and efficiently to solution of high protein concentration with minimal formation, if any, of foam, effervescence, bubbles, turbidity, or particulates that might be deleterious. The invention also provides, among other things, methods for making the lyophilized compositions. The invention in additional aspects also provides Raman Imaging Spectrographic methods for real time analyses of polymorphs in a sample using PLS algorithms. By way of particular example, the use of the method for the analysis of mannitol polymorphs is described, and the use of the analysis to determine optimum compositions and lyophilization methods for producing lyophilates of pharmaceutical proteins having a predefined distribution of mannitol polymorphs and having the aforementioned reconstitution properties is also described.

The present invention provides a method for concentrating a protein, in particular a method for concentrating a plasma product, in particular IgG, using glycine in a (two-stage ultrafiltration/diafiltration approach.

The invention discloses a process method for quickly preparing a soybean antioxidant peptide, belonging to the technical fields of deep processing of agricultural and sideline products and comprehensive utilization of byproducts thereof, and relating to the process method for quickly preparing the soybean antioxidant peptide by using a microwave technology. According to the process method, soybean protein concentrate powder with protein content of 70 percent is used as a raw material, the material passes through a microwave extractor through processes of preparing the soybean protein concentrate powder, pretreating soybean protein concentrate powder emulsion, performing microwave auxiliary enzymatic treatment on the soybean protein concentrate powder emulsion, deactivating enzymatic hydrolysate, centrifuging, performing ultrafiltration treatment and performing vacuum freeze drying treatment by using the microwave auxiliary enzymatic hydrolysis technology; the enzymatic hydrolysis time is shortened to 35-38 minutes; and compared with the conventional enzymatic hydrolysis, the efficiency is increased by 3-4 times, and the ABTS (2, 2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid)) free radical clear rate is over 72 percent and is equivalent to that of the conventional enzyme. According to the process method, a new idea is provided for technical research for quickly preparing the soybean antioxidant peptide, and a new approach is provided for development and application of soybean series products of high antioxidant activity.

A method of simultaneously co-purifying and concentrating nucleic acid and protein targets is described. The method includes automation of the entire sample preparation process, performed by having an analyst add a sample into a device that performs all of the steps necessary to prepare a sample for analysis. The method provides for samples that are not split during the sample preparation process and where common purification methods can be used for purifying multiple analytes.

Provided herein are methods for producing a mung bean protein isolate having high functionality for a broad range of food applications. In some embodiments, the methods for producing the isolate comprise one or more steps selected from: (a) extracting one or more mung bean proteins from a mung bean protein source in an aqueous solution, for example, at a pH between about 6.5-10.0; (b) purifying protein from the extract using at least one of two methods: (i) precipitating protein from the extract at a pH near the isoelectric point of a globulin-rich fraction, for example a pH between about 5.0-6.0; and/or (ii) fractionating and concentrating protein from the extract using filtration such as microfiltration, ultrafiltration or ion-exchange chromatography; and (c) recovering purified protein isolate.

The present invention is concentrated soybean protein modifying method and features that water insoluble protein obtained through low temperature separation of defatted soybean residue is produced into concentrated protein powder in high water solubility through water dilution, pH regulation, heating, homogenization, drying and other steps. The concentrated protein powder has water solubility as high as 70-80 %, protein content of 67-70 %, processing performance of being emulsified, thickened, gelatinized, etc. and expanded use range. In addition, the process of the present invention has low cost, less pollution and good product taste.

An electrophoretic cell configuration and related method are disclosed that employ oppositely directed traveling electrical waves. The waves travel across the cell and samples undergoing separation. Various strategies are used to selectively direct the movement and arrangement of the samples and resulting sample patterns.

A process for reclaiming the leftover of shrimp by enzyme method includes such steps as using the composite enzyme to hydrolyze the leftover of fresh shrimp, filtering, concentrating to obtain the concentrated protein rich in astaxanthin, and spray drying. Its product can be used to prepare the flavouring.

The invention provides a method for synchronously preparing peanut oil and peanut peptides through aqueous enzymatic extraction and belongs to the technical field of deep processing of agricultural products and comprehensive utilization of byproducts of the agricultural products, mainly solving the technical problems that protein products obtained according to a conventional technology for preparing oil from peanuts through aqueous enzymatic extraction have low purity and are imperfect in oil flavor and easy in generation of emulsion and the like. The method comprises the following steps of: baking peanut kernels, grinding, adding water so as to adjust acid for extraction, carrying out three phase centrifugation, combining oil bearing emulsion phase and protein concentrate, adjusting alkali, adding protease for enzymatic hydrolysis, carrying out high-temperature enzyme deactivation after the enzymatic hydrolysis is ended, and carrying out three phase centrifugation under a hot condition, thus directly obtaining the free oil and the peanut peptides. Peanuts are processed by adopting the method, so that the emulsion can be basically avoided, and the obtained oil has strong flavor and high quality; the peanut peptides have high purity (75% of protein content) and low relative molecular mass and can be used as a good functional additive to be widely applied to food processing.

The invention provides a low-fat high-protein yoghourt and a preparation method thereof. The yoghourt is prepared from, by weight, the following raw materials: 80-90 parts of low-fat milk, 8-12 partsof composite protein powder, 0.2-8.5 parts of a sweetener and a proper amount of a leavening agent, wherein the total weight is 100 parts, and the use amount of the leavening agent per 100 kg of the raw materials is 10-25U, and the composite protein powder is selected from at least two of concentrated whey protein, isolated whey protein, milk concentrated protein, casein and skimmed milk powder. By screening raw milk indexes, strains, composite protein powder and other auxiliary materials and optimizing the proportion, the problem is effectively solved that high-protein yoghourt products are prone to serious particles and poor in taste. The protein content of the product is 8% or above, and the product has the characteristics of strong fragrance, smoothness and fine taste. In addition, theprocessing method is simple and feasible, devices such as centrifugation, filtration and the like are not needed, and the cost is saved.

The invention discloses no-antibiotic feed for a piglet. The no-antibiotic feed comprises the following materials: expanded corns, soybean meal, expanded soybeans, flour, whey powder, fish meal, soybean concentrated protein, plasma protein powder, soybean phospholipid, sucrose, glucose, sodium chloride, calcium lactate, calcium dihydrogen phoshate, special enzyme for a baby pig, a detoxication agent, citric acid, sodium butyrate, eucommia bark extract, xylooligosaccharide, lactobacillus, a phagostimulant and compound premix, wherein the phagostimulant contains calcined oyster shell powder, fiveleaf gynostemma herb, lalang grass rhizome, pine needle, denucleated wild jujubes, malic acid, radix glycyrrhizae preparata, royal jelly freeze-dried powder and peony cake meal. The no-antibiotic feed and the preparation process disclosed by the invention have the advantages that by the feed formula, the growth performance and the immunity of the piglet can be obviously improved; and by the mode of proportioned nutrition, the diarrhea and the death rate of the piglet are reduced, the effect of preventing and treating diseases is achieved and the feeding attraction and growth-promoting effects are obvious.

Process for preparing a concentrated milk protein ingredient comprising the steps of: providing a membrane retentate solution having kappa-casein milk protein, adjusting the divalent ion content of said protein solution to a predetermined level at which no gel is formed after treatment with milk clotting enzyme, adding a food grade milk clotting enzyme under conditions where kappa-casein is converted to para-kappa casein while remaining in solution, terminating the conversion by removal or inactivation of the enzyme and concentrating said solution. The resultant milk protein concentrate ingredient is used in the production of cheese.

A method of simultaneously co-purifying and concentrating nucleic acid and protein targets into a single volume is described. The method includes automation of the entire sample preparation process, performed by having an analyst add a sample into a device that performs all of the steps necessary to prepare a sample for analysis. The method provides for samples are not split during the sample preparation process and where common purification methods can be used for purifying multiple analytes.

The invention discloses a plant immune activator protein FoPII1 secreted by fusarium oxysporum and its application. The protein is capable of inducing plant resistance and can be applied as a plant immune activator to the field of plant disease control. An amino acid sequence of the protein is shown in SEQ ID NO: 2. The high-concentration protein can be obtained by using engineering strain expression, which can be used to improve the disease resistance of tobacco and soybean, and the involved plant diseases are tobacco mosaic disease and soybean root rot. The plant immune activator protein notonly can significantly improve plant disease resistance, but also has the advantages of low required concentration, quick effect and long acting time. The FoPII1 provides a novel way to improve plantdisease resistance and control plant diseases.

The invention relates to a method for extracting soy protein, oligosaccharide and isoflavone from soybean wastewater by a one-step process. The method is characterized by comprising the steps of: obtaining protein concentrate and ultrafiltration membrane permeate liquid by an ultrafiltration membrane after wastewater pretreatment is carried out; preparing soy protein powder from the protein concentrate; eluting resin by ethanol after adsorbing the ultrafiltration membrane permeate liquid by the resin; preparing soy isoflavone from washing liquor; obtaining oligosaccharide concentrate and nanofiltration membrane permeate liquid after the resin effluent passes through a nanofiltration membrane; preparing oligosaccharide powder from the oligosaccharide concentrate; and obtaining the purified water after the nanofiltration membrane permeate liquid passes through a reverse osmosis membrane. The method has the beneficial effects that high-concentrate organic matters in bean product waste water can be thoroughly extracted. Thus, discharge water achieves grade 1 emission standard; the environment is effectively protected; and the method brings benefits to children. Meanwhile, the blank of extraction of the soy oligosaccharide, the soy protein and the soy isoflavone from the wastewater in bean product production industry in China can be filled by putting the project into production.

The invention provides a method for detoxifying barbadosnut seed dregs and preparing protein concentrates, which belongs to the field of fine and further processing of barbadosnut seed dregs. The method comprises the following steps of: destroying plant cell walls by firstly using an extruding bulking machine as a reactor and then utilizing biological enzymolysis, then eluting by using alcohol with a proper concentration, thereby solving the problem of toxin residual of the barbadosnut seed dregs and simultaneously preparing the protein concentrates of the barbadosnut seed dregs; and finally, carrying out nutrition evaluation on the protein concentrates of the barbadosnut seed dregs. The method has the advantages of low requirement on equipment, simple production process, low production cost and environmental friendliness without using strong acid and strong base for treating the barbadosnut seed dregs, also has low cost of raw materials and low production cost and is suitable for large-scale industrial production. Meanwhile, the method also meets the requirements of fine and further processing field of agricultural products in the 'National Guideline on Medium- and Long-Term Program for Science and Technology Development' and has very obvious social and economic benefits and significance.

The invention discloses a processed cheese spread and a preparation method thereof. The preparation method comprises the steps of (1) evenly mixing 15%-25% of natural cheese, 11%-30% of milk fat, 0.2%-0.5% of emulsifying salt, 1%-5% of milk protein concentrate, 8%-12% of a sweetener, and a stabilizer and water together, (2) further adding 1-15% of kiwi fruit pulp, 1-4% of apple concentrated juice, 0.5-1.2% of cucumber concentrated juice, 0.03-0.42% of an acidity regulator and 0.02%-0.04% of an antioxidant at a stirring speed ranging from 600rpm to 1200rpm, and stirring, (3) carrying out homogenizing at a temperature ranging from 82 DEG C to 90 DEG C and under pressure within the range from 15MPa to 20MPa, and pasteurizing, and (4) performing super-high pressure treatment on 0.05-0.2% of fresh vegetable juice, and then adding the fresh vegetable juice to the slurry obtained in the step (3), and carrying out hot filling and three-stage quick cooling and molding. The processed cheese prepared by the preparation method is high in nutritive value, naturally green, and good in sensory evaluation.

The present invention relates to fluorescent viscosity probes with aggregation-induced luminescence properties, applications of the probes in solution viscosity detection, glucose concentration determination, viscosity (free volume) change monitoring during polymerization, and viscosity measurement in cells or mitochondria, and an application method. The probes may consist of one or more aggregation-induced luminescence fluorophores. Compared with commercial DCVJ, the probes have higher viscosity sensitivity. The probes are two-photon-excitable and have specific mitochondrial targeting properties so that the probes can be applied in biological research. In addition, the probes can be used for indicating mitochondrial membrane potentials, quantitatively measuring low-concentration proteins,bacterial imaging, mechanical force-induced fluorescence discoloration materials, and other applications.

Herein is described and optimum solubility screen in which a panel of buffers and many additives are provided in order to obtain the most homogeneous and monodisperse protein condition for protein crystallization. The present methods are useful for proteins that aggregate and cannot be concentrated prior to setting up crystallization screens. A broad range of buffers is intended for use in this screen. A high-throughput method using the hanging-drop method and vapor diffusion equilibrium and a panel of twenty-four buffers is further provided. After monitoring precipitation, the conditions leading to clear drops are selected for evaluation, preferably dynamic light scattering (DLS) characterization. If the DLS results are not optimal, a series of additives are tested in the presence of the best buffer selected from the initial screen and again DLS is used to determine the best condition. Using the present methods, 14 poorly behaving proteins have been screened, resulting in 11 of the proteins having highly improved DLS results allowing concentration of the proteins, and 9 were crystallized.

The invention discloses a method for synchronously extracting soybean oil and protein concentrate, belonging to the vegetable oil extracting and processing technology. The method comprises the following steps: performing enzymolysis on the soybean meal after extrusion puffing, centrifuging and collecting the hydrolyzate; adding ethanol into the hydrolyzate, performing cold bath, centrifuging and precipitating to obtain high-purity protein concentrate; and (2) rotating and evaporating the supernate, performing ultrasonic and high-speed centrifugal treatment on the residual mixture, and leadingout and collecting the oil to obtain the soybean oil. The method can synchronously obtain high-quality green soybean oil and high-purity protein concentrate, requires simple equipment, is safe to operate, avoids solvent residual in the obtained soybean oil, obtains high-quality oil with high nutritional value, and can realize an oil recovery rate of 99-100%.