Method for extracting soy protein, oligosaccharide and isoflavone from soybean wastewater by one-step process

A technology of soybean product wastewater and soybean isoflavones, applied in the field of extracting nutrients, can solve the problems of product decolorization, complex operation and comprehensive utilization treatment, large amount of organic solvent, etc., and achieve the effect of protecting the environment

Inactive Publication Date: 2013-08-28
YIBIN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the process involved is long, the amount of organic solvent added is large, the operation is complicated, and the soybean protein and soybean oligosaccharides in the soybean whey wastewater have not been further comprehensively utilized and processed, and the problem of product decolorization has not been solved.
[0010] So far, there have been

Method used

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  • Method for extracting soy protein, oligosaccharide and isoflavone from soybean wastewater by one-step process
  • Method for extracting soy protein, oligosaccharide and isoflavone from soybean wastewater by one-step process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1: as figure 1 Shown:

[0036] Add 101 metal remover to the yellow water discharged from soybean products, send it into a bag filter and filter it through a microfilter to obtain clarified yellow water discharged.

[0037] Under the conditions of temperature 10-45°C and pressure 0.5-6 Pa, the clarified and discharged yellow water is sent to the ultrafiltration membrane, and the ultrafiltration membrane cuts off between 6000Da and 10000Da, so the soybean protein liquid and permeate are separated and concentrated.

[0038] The concentrated soybean protein liquid is spray-dried to obtain white soybean protein powder.

[0039] Ultrafiltration membrane permeate (containing soy isoflavones and oligosaccharides) absorbs soy isoflavones through weakly polar macroporous resin at a flow rate of 2-4BV / h. Use 20-30% ethanol and 70-80% ethanol to elute the soybean isoflavones adsorbed by the resin respectively, combine the lotions, concentrate under reduced pressure and...

Embodiment 2

[0043] Embodiment 2: as figure 2 Shown:

[0044] Add 101 metal remover to the yellow water discharged from soybean products, send it into a bag filter and filter it through a microfilter to obtain clarified yellow water discharged.

[0045] Under the conditions of temperature 10-45°C and pressure 0.5-6 Pa, the clarified and discharged yellow water is sent to the ultrafiltration membrane, and the ultrafiltration membrane cuts off between 6000Da and 10000Da, so that soybean protein liquid and permeate are separated.

[0046] The concentrated soybean protein liquid is spray-dried to obtain white soybean protein powder.

[0047] The ultrafiltration membrane permeate (containing soybean isoflavones and oligosaccharides) is sent to the nanofiltration membrane at a temperature of 25-45°C and a pressure of 1.5-6 Pa. The nanofiltration membrane cuts off between 66Da-300Da, so it is separated and concentrated to obtain Soybean oligosaccharide concentrate, soy isoflavone concentrate a...

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Abstract

The invention relates to a method for extracting soy protein, oligosaccharide and isoflavone from soybean wastewater by a one-step process. The method is characterized by comprising the steps of: obtaining protein concentrate and ultrafiltration membrane permeate liquid by an ultrafiltration membrane after wastewater pretreatment is carried out; preparing soy protein powder from the protein concentrate; eluting resin by ethanol after adsorbing the ultrafiltration membrane permeate liquid by the resin; preparing soy isoflavone from washing liquor; obtaining oligosaccharide concentrate and nanofiltration membrane permeate liquid after the resin effluent passes through a nanofiltration membrane; preparing oligosaccharide powder from the oligosaccharide concentrate; and obtaining the purified water after the nanofiltration membrane permeate liquid passes through a reverse osmosis membrane. The method has the beneficial effects that high-concentrate organic matters in bean product waste water can be thoroughly extracted. Thus, discharge water achieves grade 1 emission standard; the environment is effectively protected; and the method brings benefits to children. Meanwhile, the blank of extraction of the soy oligosaccharide, the soy protein and the soy isoflavone from the wastewater in bean product production industry in China can be filled by putting the project into production.

Description

technical field [0001] The invention relates to a method for extracting nutrients from waste water in food processing industry. Background technique [0002] In the bean product industry, the source of brine product wastewater mainly comes from the soaking, washing, pulping and pressing wastewater of raw soybeans. During the above process, the soybean protein, soybean oligosaccharides and soybean isoflavones in soybeans are partially dissolved In the water, the wastewater has high organic content and strong biodegradability. Direct discharge will significantly increase the COD (chemical oxygen demand) and BOD (biological oxygen demand) values ​​of the water quality. In severe cases, it will cause suffocation of aquatic animals and plants in rivers. Rot, thereby destroying our own survival and living environment, so the above-mentioned wastewater is the most serious high-concentration organic wastewater that pollutes the environment. However, the cost of wastewater treatment...

Claims

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Application Information

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IPC IPC(8): C07K1/34C07H3/06C07H1/08C07D311/36C07D311/40
Inventor 王健王金月姜波
Owner YIBIN UNIV
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