44 results about "SOY ISOFLAVONES" patented technology

The invention relates to the field of gene engineering, in particular to a daidzein reductase mutant and application of the daidzein reductase mutant in preparation of equol, and the daidzein reductase mutant is prepared by performing one or more of the following mutations on the basis of a wild type: V263F, P107A, H351R, G35L, H171A, A267Q, H351Q, M355Q, H351P, G31V, V176A, H351Y, N64K, V137A, H351W, D63A, W257A and C174N. The catalytic activity of the mutant obtained through precise mutation is remarkably improved under the feedback inhibition condition, especially when high-concentration equol exists, the mutant can still keep high relative activity, and continuity and high efficiency of the biosynthesis process are ensured. When the mutant is applied to biosynthesis of equol, the production titer is remarkably improved, the yield can be improved by about 1.2 times compared with that of a wild type, and the economic benefit and the yield of biosynthesis are directly improved.

A probiotic and prebiotic-embedded synbiotic system for targeted delivery and colonization of functions, comprising a probiotic core; an inner layer of protein-polysaccharide / oligosaccharide complex wrapped around the probiotic surface, which acts as both an adhesion promoter, promoting the attachment of probiotics to the intestinal epithelial cells, and as a prebiotic, promoting the growth of probiotics upon release; and an outer layer of a natural emulsifying system on the synbiotic complex, which minimizes water infiltration, thereby effectively embedding and protecting the probiotics. The present synbiotic system is suitable for production in powder form using freeze-drying technology, and is suitable as a dietary supplement or for addition to any food in dry powder form. The system has the ability to bioconvert soy isoflavones and release bioactive phenolic acids from grains to promote body health.

[Problem] To improve production efficiency in the breeding and farming of crustaceans.　[Solution] Provided are a method for improving the survival rate of crustaceans, a method for producing crustaceans, etc., in which a soybean-isoflavone-containing crustacean feed is fed to crustaceans. In the breeding and farming of crustaceans, a high survival rate during the breeding period can be maintained by feeding a soybean-isoflavone-containing crustacean feed to the crustaceans. Specifically, the number of deaths of crustaceans introduced into breeding and farming can be reduced, and, for example, the number of crustaceans that grow to a level suitable for shipping can be increased; therefore, the production efficiency in the breeding and farming of crustaceans can be improved.

The invention relates to the technical field of microbial fermentation engineering, and particularly discloses natto red yeast rice herb bean flour and a preparation method thereof. The preparation method comprises the following steps: respectively activating specifically preserved bacillus natto and monascus to obtain secondary seed solutions; inoculating the two seed solutions into a solid-state fermentation culture medium for co-fermentation; and inactivating, drying and crushing the fermentation product to obtain a finished product. The solid-state fermentation culture medium is prepared by compounding a bean matrix (soybeans, black beans or chickpeas) and herb tea components (roses, lotus leaves, chrysanthemums, mulberry leaves, haws, cassia seeds and the like). Through the synergistic effect of the bacillus natto and the monascus, nattokinase and monacolin K are efficiently enriched in a single fermentation system at the same time, conversion of soy isoflavone to a high-bioavailability aglycone type is remarkably promoted, and the obtained product has multiple health-care functions of thrombolysis, lipid reduction, efficient absorption and the like.

The invention provides a pulping control method and device, a storage medium and a food processer, the method is applied to the cooking field, and the method comprises the following steps: controlling a heating device, a stirring device or a ventilation device of the food processer to work according to different control parameters in different cooking stages in the pulping process of the food processer, oxygen-containing gas is introduced into the water-material mixture in the cooking cavity through the ventilation device, so that glucoside-type soy isoflavone released in the water-material mixture generates free isoflavone under the action of endogenous enzyme, and the nutritional value of the finally prepared slurry is improved; meanwhile, the Maillard reaction of protein and reducing sugar in the water-material mixture can be promoted, and the aroma and taste of the finally prepared pulp are improved.

This invention discloses the application of plant extracts in the preparation of drugs that inhibit canine parvovirus, belonging to the field of biomedical technology. The plant extract is at least one selected from phlorizin, neomethylhesperidin-dihydrochalcone, tribulus terrestris saponin, stevia glycoside, soy isoflavones, berberine hydrochloride, diosgenin, and silymarin. Through in-depth research on various plant extracts, this invention has found that phlorizin, neomethylhesperidin-dihydrochalcone, tribulus terrestris saponin, stevia glycoside, soy isoflavones, berberine hydrochloride, diosgenin, and silymarin all exhibit significant inhibitory effects on canine parvovirus, with soy isoflavones showing particularly outstanding effects. This research provides a novel natural drug option for the prevention and treatment of canine parvovirus. This invention not only expands the application scope of natural plant extracts in the veterinary medicine field but also provides important technical support for the development of novel, highly effective, safe, and side-effect-free drug formulations for the prevention and treatment of canine parvovirus.

This invention discloses an S-estrol composition for delaying ovarian aging and its uses, relating to the fields of biomedicine and health products. The S-estrol composition for delaying ovarian aging comprises, by weight, the following components: 1-5 parts S-estrol, 2-8 parts soy isoflavones, 3-10 parts coenzyme Q10, 2-6 parts vitamin E, 4-12 parts vitamin C, 0.01-0.05 parts folic acid, 0.1-0.3 parts zinc source, 0.5-1.5 parts anti-aging protective agent, 50-80 parts carrier, and 10-15 parts purified water. This invention uses high-purity S-estrol as the core, combined with soy isoflavones, coenzyme Q10 and a variety of antioxidant nutrients in a scientific formulation to form a synergistic system for estrogen receptor signal regulation and antioxidant defense. By constructing a complex antioxidant network and introducing novel anti-aging protectants for synergistic effect, the activity of antioxidant enzymes tends to increase and the level of lipid peroxidation tends to decrease, thereby improving the oxidative stress state in ovarian cells as a whole.

The invention relates to a liquid microbial enzyme as well as a preparation method and application thereof. The liquid microbial enzyme is prepared by taking soybean meal, fish meal, rice husk and water as raw materials, sterilizing and fermenting through a compound microbial agent, and the compound microbial agent comprises bacillus subtilis, bacillus licheniformis and bacillus velezensis. In the application, the soybean meal serves as a core nitrogen source and an energy source, soybean isoflavone of the soybean meal promotes functional bacteria to proliferate and enhances enzyme activity, and a fiber structure can optimize fermentation conditions; the fish meal provides high-quality protein which can be decomposed to generate organic acid, so that the pH value is reduced, and putrefying bacteria are inhibited; the rice husks maintain the material structure, and the porous characteristic improves ventilation and adsorbs peculiar smell; the bacillus velezensis as an auxiliary enzyme source can enhance the variety coverage of soluble enzymes, so that the substrate degradation capacity is more comprehensive. After the liquid microbial enzyme is prepared, the liquid microbial enzyme and the hermetia illucens are combined, livestock and poultry manure is used as a conversion object, and the conversion efficiency of the livestock and poultry manure on the manure is improved by regulating and controlling intestinal microflora of larvae of the hermetia illucens.

The present application relates to the technical field of traditional Chinese medicine composition, and discloses a traditional Chinese medicine composition for relieving rheumatoid arthritis and a preparation method thereof, which comprises Qingfengteng, Huangqi, Chuanshanyang, propolis, turmeric, Fuling, soybean isoflavone peptide, chitosan, Gancao, Chufu, Guizhi and Dangui, and the preparation method comprises raw material pretreatment, extraction, active ingredient mixing and preparation forming. The traditional Chinese medicine composition prepared by the present application has a maximum inhibition rate of 88.6% and 86.4% on TNF-alpha and IL-6, a bioavailability of 87.5%, and can improve immune function and quickly relieve joint swelling and pain. Moreover, the preparation process of the composition is stable and suitable for industrial production, and the composition has significant clinical application value in the treatment of rheumatoid arthritis.

The invention discloses a method for producing high-activity soybean isoflavone by fermenting soybean milk through lactobacillus pentosus and a solid beverage prepared through the method, and relates to the technical field of microbial fermention.The method comprises the following steps that the soybean milk is inoculated with the lactobacillus pentosus 068-1, fermentation is conducted for 36-60 h at the temperature of 32-40 DEG C, and the high-activity soybean isoflavone is obtained. After fermentation with lactobacillus pentosus, the soybean isoflavone is hydrolyzed from combined soybean isoflavone to free soybean isoflavone, the fermentation process is simple and easy to control, and the method has the advantage of high repeatability of microbial fermentation, is not influenced by territory and environmental factors, and has a good hydrolysis effect.

This invention provides a composition containing soy isoflavones, its preparation method, and its application, relating to the technical field of cosmetics. The soy isoflavone-containing composition includes soy isoflavones, an oil phase matrix, liposome excipients, a transdermal absorption enhancer, a moisturizer, an emulsifier, an antioxidant, a chelating agent, and a preservative; wherein the liposome excipients include a first liposome excipient serving as a membrane framework and a second liposome excipient serving as a membrane modulator. The composition of this invention significantly improves the transdermal absorption efficiency of soy isoflavones in cosmetics, achieving a synergistic effect of multiple functions such as oil control and acne treatment, moisturizing and repairing, brightening, and anti-aging; simultaneously, it further reduces skin irritation, improving the safety and effectiveness of the product in people with sensitive and acne-prone skin.

The invention relates to the technical field of biological medicine and health care, in particular to a production method and formula for balancing female hormone level, and the production method comprises the following steps: mixing plant extract, microbial metabolite and functional lipid to form a basic active mixture, adding a trace element compound, and performing microencapsulation treatment. The composition comprises an evening primrose oil extract, a soy isoflavone extract and the like, and accurate regulation and control are realized by regulating a hormone synthesis route and signal transduction. The invention further provides an application method and an effect evaluation mode, the formula proportion can be adjusted according to individual hormone detection results, and the composition is suggested to be taken after breakfast so as to improve the absorption efficiency. The personalized health management requirement can be efficiently and safely met, and meanwhile the long-term effect stability is improved.

The invention provides a pulping control method and device, a storage medium and a food processer, the method is applied to the cooking field, and the method comprises the steps that a stirring device and a heating device of the food processer are controlled to work according to different control parameters in different cooking stages in the pulping process of the food processer, introducing air flow into a cooking cavity of the food processor in at least one of the first cooking stage and the third cooking stage so as to introduce oxygen-containing gas into the water-material mixture in the cooking cavity, so that glucoside-type soy isoflavone released in the water-material mixture generates free isoflavone under the action of endogenous enzyme; according to the present invention, the nutritional value of the finally prepared slurry is improved, the rich protein, the reducing sugar and other sweet substances are released during the first cooking stage and the second cooking stage, the Maillard reaction of the protein and the reducing sugar in the water-material mixture can be promoted, and the aroma and the sweet taste of the finally prepared slurry are improved.

The application discloses a kind of compound enzyme and its application, belong to the field of biotechnology.The compound enzyme provided by the application includes: pectinase 5-15 parts by weight, glucose glycosidase 5-15 parts, glycerol 2-8 parts and water 15-20 parts, the compound enzyme also includes phytase 2-6 parts, lactic acid dehydrogenase 1-4 parts.The compound enzyme provided by the application can significantly improve the extraction rate of soybean isoflavones, and the content of isoflavones is significantly improved.

The invention discloses a method for purifying S-equol in genetic engineering escherichia coli fermentation liquor. According to the method, daidzein is used as a fermentation substrate, and S-equol-producing genetic engineering escherichia coli is used for fermenting escherichia coli BL21-G2 to obtain fermentation liquor; then, according to the characteristic of high polarity of the S-equol, extracting the S-equol generated by fermentation from the liquid phase by using ethyl acetate; and finally, separating and purifying S-equol and other soy isoflavones by using a specific ratio of chloroform and methanol chromatography liquid through a silica gel column, further separating and purifying S-equol and other impurities in the fermentation liquid through an ethyl acetate chromatography liquid, and finally obtaining S-equol with the purity close to 100%. Compared with a traditional ethanol column-passing extraction method, a one-step silica gel column-passing separation method and the like, the method is economical, practical and good in effect, the purity of the obtained S-equol is higher, and the process research prospect is wide.

The utility model relates to soybean isoflavone processing technical field especially relates to a soybean isoflavone raw material screening and cleaning device, it includes the soybean cleaning cylinder for soybean isoflavone raw material screening, and the inside of soybean cleaning cylinder is provided with screening and cleaning subassembly, screening and cleaning subassembly includes hollow shaft, and the inside of soybean cleaning cylinder is rotatably installed in hollow shaft, and the lower pipe and the upper pipe are installed in the circumferential equidistance communication of hollow shaft lateral wall, the bottom of lower pipe and the upper side of upper pipe all are screw -jointed installation a plurality of stirring vertical pipes, and a plurality of gas holes are seted up on the lateral wall of stirring vertical pipe, and the side of soybean cleaning cylinder is provided with air pump, and the output end of air pump is connected and installed with gas pipe, and the lower end of gas pipe is fixedly installed with sealing sleeve, and the upper end of hollow shaft is rotatably connected with sealing sleeve, the utility model discloses through setting screening and cleaning subassembly, adopt the comprehensive impurity removal mode of soaking, stirring and pneumatic suspension, avoid the drawback of traditional filter screen blockage, improve the cleaning efficiency of soybean.

The invention discloses sea cucumber peptide health-care food with liver protection activity and a preparation method thereof, and belongs to the technical field of health-care food. The health-care food comprises the following raw materials in parts by weight: 80-90 parts of sea cucumber peptide, 5-12 parts of a Chinese magnoliavine fruit extract, 2-8 parts of a cassia seed extract, 0.5-2 parts of vitamins and 0.1-0.5 part of soy isoflavone. The preparation method comprises the following steps: firstly, respectively preparing the sea cucumber peptide, the schisandra chinensis extract and the semen cassiae extract, and then mixing, drying and forming all the raw materials. The sea cucumber polypeptide is prepared from the sea cucumber byproducts, so that high-value utilization of resources is realized; all the raw material components are scientifically matched, the obvious liver protection effect is achieved through multi-target and multi-way synergism, and the product is high in safety and suitable for daily health care and auxiliary conditioning of people suffering from liver diseases. The process is simple and feasible, and is suitable for industrial production.

The invention discloses a nutritional supplement for pregnant pets and a preparation method of the nutritional supplement. According to the preparation method, after chickpeas are hydrolyzed by protease, GAD enzyme and calcium chloride are added, glutamic acid is induced to be converted into GABA through calcium ions, and meanwhile free amino acid reacts with the calcium ions to generate amino acid chelated calcium; the egg yolk powder and the lactoferrin are utilized to construct a double-emulsion system, so that the GABA is protected from being damaged in advance, the amino acid chelated calcium is slowly released, and the absorption antagonism of mineral substances such as calcium, iron and zinc is reduced. The chicken breast, the chicken liver, the traditional Chinese medicines and other raw materials are chopped, mixed and sterilized to obtain a finished product. The nutritional powder is rich in GABA, soybean isoflavone, Omega-3 fatty acid, vitamin B6, lactoferrin, folic acid and various minerals, can directly or indirectly support progestational hormone secretion and relieve anxiety during pregnancy, meets the requirements of maternal blood replenishing and immunity improving and fetal development nutrition supply, and is suitable for nutrition supplement of pets during pregnancy.

The utility model discloses a soy isoflavone plant essence extracting device, and particularly relates to the technical field of bean processing, the soy isoflavone plant essence extracting device comprises a soy isoflavone extracting device, an extracting container is arranged on the soy isoflavone extracting device, a placing groove is formed in the bottom end of the soy isoflavone extracting device, the extracting container is placed above the placing groove, and the extracting container is arranged above the placing groove. A sliding groove is formed in the bottom end of the soybean isoflavone extraction equipment, a sliding block is slidably connected into the sliding groove, a stabilizing rod is fixedly connected into the sliding groove, the stabilizing rod is slidably sleeved with the sliding block, a clamping pad is arranged on the inner surface of a clamping block in an attached mode, and the clamping pad is attached to the outer wall of an extraction container. Through the design of the placing groove, the anti-skid lines, spring clamping and the like, the stability of the extraction container is improved, the manual operation cost is reduced, manual supporting by personnel is not needed, the extraction efficiency is improved, the stability of the extraction process is guaranteed, and the equipment operation efficiency can be effectively improved.

The invention belongs to the technical field of medicines and disease treatment, and particularly relates to application of genistein to preparation of a medicine for improving brain cognitive impairment caused by low pressure and low oxygen. Research results show that low pressure and low oxygen can cause brain cognitive dysfunction of mice. The genistein can relieve brain dysfunction after low-pressure and low-oxygen exposure of mice by inhibiting neuroinflammation, recovering hippocampal neurological functions and improving hippocampal oxidative aggression. The invention provides a novel therapy for brain cognitive impairment caused by low pressure and low oxygen in various leguminous plant components, which is mainly derived from soy isoflavone by verifying a pharmaceutical active component, namely genistein.

The invention relates to the technical field of biological medicine, and discloses an exosome preparation for treating thin endometrium and a preparation method thereof, and the preparation method comprises the following steps: step 1, culturing mesenchymal stem cells; step 2, extracting an exosome; step 3, preparing a collagen solution; step 4, preparing traditional Chinese medicine components; and step 5, uniformly stirring the collagen solution and the sheep placenta powder extracting solution under the condition of 4 DEG C, then adding the exosome solution, mixing, then adding the soy isoflavone solution and auxiliary materials, fully reacting to obtain exosome gel, and storing under the condition of 4 DEG C. According to the exosome preparation for treating the thin endometrium and the preparation method of the exosome preparation, collagen gel is used as a slow-release matrix, three-dimensional structure support is provided for active substances, degradation of the active substances is slowed down, meanwhile, growth factors in the sheep fetus powder extracting solution and the exosome act together, revascularization is activated, blood supply is improved, and the effect of treating the thin endometrium is achieved. The regrowth of the endometrium is promoted.

The invention discloses an acid soil conditioner as well as a preparation method and application thereof, and belongs to the technical field of soil improvement. The acid soil conditioner is prepared from the following raw materials in parts by weight: 25 to 35 parts of rice hull biochar, 15 to 25 parts of humic acid, 10 to 20 parts of soy isoflavone and 15 to 25 parts of bean dreg fermentation products. The prepared acid soil conditioner has a good treatment effect on acid soil, few raw material components are used, the preparation process is simple, the pH value of the acid soil can be reduced, the carbon sequestration and emission reduction effects of the acid soil are improved, and application and popularization are facilitated.

The invention discloses a breed selection and cultivation method for increasing the egg yield of a Wulong goose group, and relates to the technical field of poultry breeding, and the method comprises the following steps: S1, a hatched seedling stage; s2, a young goose stage; s3, a production starting preparation period; s4, an adult goose stage. Soybean isoflavone and flaxseeds are fed to promote the synthesis of E2 and TG, and low-dose diethylstilbestrol and fish oil are added into the feed after the geese are 180 days old, so that the generation of TG is promoted. Soybean isoflavone serves as phytoestrogen, ovarian granular cell proliferation and follicle development related genes can be activated, primary follicles are promoted to be converted into mature follicles, and the storage life of a follicle library is prolonged; diethylstilbestrol is used as a synthetic estrogen, so that the hypothalamus-pituitary-ovarian axis function can be accurately adjusted, ovulation synchronization is promoted, the egg laying interval prolonging caused by disordered ovulation is avoided, the follicular cell function of an individual with the hypoovarian function is repaired, and the egg laying capacity is recovered.

The invention provides a method for optimizing ultrasound-assisted compound enzyme enrichment daidzein based on a response surface method. The method comprises the following steps: dissolving soy isoflavone in a phosphate-citric acid buffer solution; the method comprises the following steps: carrying out enzymolysis on soy isoflavone by using ultrasonic-assisted compound enzyme, after the reaction is finished, inactivating the enzyme in a boiling water bath, cooling to room temperature, extracting, separating by using macroporous adsorption resin, eluting by using water and ethanol, concentrating the eluent by using a rotary evaporator, and carrying out vacuum freeze drying to obtain daidzein powder. According to the method, daidzein is enriched by adopting an ultrasonic-assisted enzymolysis method, and soy isoflavone enzymolysis is subjected to process optimization by combining a single-factor experiment with a response surface test. The method provided by the invention realizes higher resource utilization rate and lower environmental pollution risk, and conforms to the concepts of green chemistry and sustainable development; the defect that in the prior art, damage to daidzein is large is overcome, and the enzymolysis rate of soy isoflavone and the purity of daidzein can be remarkably increased.

The invention relates to the technical field of food processing, in particular to a method for preparing crisp radish peel from bean curd yellow water. The invention relates to a method for preparing crisp radish peel by using yellow bean curd water. The crisp radish peel comprises the following raw materials: yellow bean curd water, radish peel, edible acid, table salt, sugar and spices. The method has the beneficial effects that soluble protein and carbohydrates in yellow water promote lactobacillus fermentation, and the crispness and flavor of the radish peel are improved; the yellow water is rich in soy isoflavone and oligosaccharide, so that the functionality of the product is improved; the traditional pickling salt dosage is reduced, and the health requirement is better met; waste is turned into wealth, and tofu industry pollution is reduced.

This invention provides a pet functional food formula and its preparation method that can effectively activate pet joints. The pet functional food comprises the following components in parts by weight: 10-13 parts mussel powder, 4-8 parts plant extract, 5-9 parts modified starch, 6-9 parts egg yolk powder, 10-15 parts milk powder, 0.2-0.4 parts soy isoflavones, 1-2 parts chondroitin, 4-6 parts basa fish meat, 3-8 parts carrot, 1-2 parts salt, 2-6 parts peanut oil, and 1-5 parts vitamin A. The product prepared from the pet functional food formula of this invention effectively reduces osteoarthritis in dogs, improves coat shine, and exhibits good storage stability.

The application discloses a method for improving the proportion of genistein in soybean isoflavone glycosides through directional fermentation. Lactobacillus sp. The method is characterized in that Lactobacillus (RS-03027) is used for fermentation after induced pre-culture, and the substrate is fed in batches, so that high selective conversion of isoflavone glycosides to genistein is realized. The strain has high beta-glucosidase activity related conversion capacity, has been preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.21302. The process realizes high accumulation and high selective enrichment of the target product, effectively inhibits non-target aglycone, and has industrial amplification application potential.

The invention belongs to the technical field of soybean milk powder, and particularly relates to a dendrobium-containing soybean milk powder composition which comprises the following components: 70-85% of soybean milk powder, 3-10% of a dendrobium extract, 5-15% of maltose and 2-8% of fructo-oligosaccharide, and specifically comprises the following components: 70% of soybean milk powder, 10% of a dendrobium extract, 12% of maltose and 8% of fructo-oligosaccharide. The dendrobium extract is an extract obtained by water extraction of dendrobium stems, and the solid content is 20-50%; in the dendrobium-containing soybean milk powder composition disclosed by the invention, the soybeans provide high-quality protein and soy isoflavone; the dendrobe extract is rich in dendrobe polysaccharide and alkaloid, so that the immune regulation capability of a body can be remarkably enhanced, and the gastrointestinal absorption function is improved; the fructo-oligosaccharide serving as prebiotics can promote reproduction of beneficial bacteria in intestinal tracts and improve the micro-ecological environment of the intestinal tracts.

The invention relates to application of a soybean GmSAUR gene in regulating and controlling the content of isoflavone in plants, and belongs to the technical field of gene engineering. In order to improve the content of plant isoflavone and solve the technical problems existing in the prior art for improving the content of plant isoflavone, expression of GmSAUR protein in soybean seeds is proved by cultivating soybean plants with overexpressed GmSAUR genes, and the content of isoflavone in the soybean seeds with overexpressed GmSAUR genes is measured by using a high performance liquid chromatography method. It is found that overexpression of the GmSAUR gene can significantly increase the content of genistin and daidzin. In addition, it is proved through fluorescent quantitative PCR that the GmSAUR gene promotes expression of downstream isoflavone synthesis related genes. The discovery not only helps to reveal the molecular mechanism of soybean isoflavone synthesis, but also provides a potential molecular target for improving the soybean quality and increasing the nutritional value of the soybean.