101 results about "SOY ISOFLAVONES" patented technology

The invention discloses a soybean yellow serofluid fermentation product rich in aglycone type soybean isoflavone as well as a preparation method and application of the soybean yellow serofluid fermentation product, and relates to the technical field of microbial fermentation. The preparation method comprises the following steps: inoculating double yeasts into a soybean yellow serofluid culture medium rich in glucoside type soybean isoflavone, adjusting the pH value of the soybean yellow serofluid culture medium, and performing constant-temperature fermentation to obtain a soybean yellow serofluid fermentation product; the double yeast is prepared from Wickerhamomyces anomalus NNB-1 and Candida utilis LYY1 according to a proportion, the Wickerhamomyces anomalus NNB-1 is preserved in the China Center for Type Culture Collection on April 3, 2023, and the preservation number is CCTCC (China Center for Type Culture Collection) NO: M2023451. The invention further discloses a preparation method of the double yeast for the Wickerhamomyces anomalus strain capable of improving the yield of the Wickerhamomyces anomalus strain capable of improving the yield of the Wickerhamomyces anomalus strain capable of improving the yield of the Wickerhamomyces anomalus strain capable of improving the yield of the Wickerhamomyces anomalus strain. According to the present invention, the soybean yellow serofluid rich in glucoside type soybean isoflavone is adopted as the fermentation substrate, and the Wickerhamomyces anomalus NNB-1 and the Candida utilis LYY1 are subjected to double-yeast fermentation so as to significantly improve the content of daidzein, daidzein, genistein and other aglycone type soybean isoflavone in the soybean yellow serofluid fermentation product.

The invention belongs to the field of agriculture, and discloses an extraction method of soybean isoflavone, which comprises the following steps: taking soybean straws as raw materials, and carrying out ultrasonic-assisted alcohol extraction to obtain soybean isoflavone; the extraction rate of the soy isoflavone is not lower than 6mg / g. By means of the method, a considerable amount of soybean isoflavone can be extracted from the soybean straw, and the cognition on the extraction rate of the soybean straw and the soybean isoflavone in the prior art is far better than the cognition on the extraction rate of the soybean straw and the soybean isoflavone. Meanwhile, the invention further provides new application of the soybean straw.

The invention relates to the technical field of cereal milk tea preparation, in particular to sea-buckthorn cereal milk tea and a preparation method thereof.The method comprises the steps that sodium caseinate is stirred and dissolved in deionized water and then subjected to water bath to obtain a sodium caseinate solution, and soy isoflavone is fused into the cooled sodium caseinate solution to obtain a film forming solution; performing vacuum spray drying on the hippophae rhamnoides fruit solution through a vacuum spray dryer to obtain hippophae rhamnoides fruit powder particles, and mixing the film-formed fried rice, the hippophae rhamnoides fruit powder particles and ingredients to obtain the hippophae rhamnoides fruit grain milk tea, determining whether the preparation stability of the hippophae rhamnoides fruit grain milk tea meets the requirement or not based on the maximum difference quantity of the thickness of the film layer on the surface of the film-formed fried rice; determining whether the immersion depth of the stirring blades needs to be increased or not; determining whether the inclination angle of the drum needs to be reduced; and determining the number of cyclic shearing times based on the volume average particle size of the sea buckthorn fruit powder particles. The preparation stability of the hippophae rhamnoides fruit grain milk tea is improved.

The invention relates to the technical field of medical preparations, and particularly discloses a selenium-based anti-postmenopausal osteoporosis medicine and a preparation method and application thereof.The selenium-based anti-postmenopausal osteoporosis medicine comprises a main active component, a secondary active component and an auxiliary active component, the main active component is a nano-selenium-phytoestrogen compound and is formed by covalent coupling of selenomethionine and soy isoflavone, the particle size of the main active component is 50-200 nm, and the particle size of the auxiliary active component is 100-200 nm; the mass of the selenium element accounts for 5-30% of the mass of the compound; the auxiliary active component is a collagen peptide-calcium chelate and is prepared from marine collagen peptide and calcium citrate through chelation reaction, and the mass ratio of the calcium element to the collagen peptide is (1: 3)-(1: 5); the targeted delivery carrier is folic acid modified chitosan nanoparticles and is used for loading the main active component and the auxiliary active component; the auxiliary materials comprise a disintegrating agent, a lubricating agent and a pH-sensitive enteric coating material; through the synergistic effect of the main active ingredient nano-selenium-phytoestrogen compound and the auxiliary active ingredient collagen peptide-calcium chelate, bone formation can be promoted and bone resorption can be inhibited at the same time, and dual-function treatment is achieved.

The invention relates to the technical field of daily chemical products, in particular to a composition with a tightening effect and application of the composition in the daily chemical products. The composition is prepared from the following raw materials: a vine calli extracting solution, a caramel pedicel extracting solution, a coconut tomato extract, soy isoflavone, a bacillus fermentation product, an aloe barbadensis extract, a centella asiatica leaf extract, hamamelis virginiana water, a radix sophorae flavescentis extract, a fructus cnidii extract, a folium artemisiae argyi extract, a chamomile extract and a rose flower extract. And a scutellaria baicalensis root extract and a solubilizer. The composition disclosed by the invention adopts various natural plant extracts, the safety is high, various combinations are matched with one another, a synergistic tightening effect is achieved, and the added tenuikalii extracting solution and kacip flower pedicel maca extracting solution have remarkable tightening and beautifying effects.

The invention belongs to the technical field of cell culture, and particularly relates to a serum-free mesenchymal stem cell culture medium and a preparation method thereof. The serum-free mesenchymal stem cell culture medium provided by the invention is prepared by taking a DMEM (Dulbecco Modified Eagle Medium) / F12 basal culture medium as a basis and adding supplementary factors, namely yeast hydrolysate, insulin, albumin, dexamethasone, taurine, salidroside, soy isoflavone, baicalein, human platelet lysate, momordica saponin and hyperoside. According to the serum-free mesenchymal stem cell culture medium provided by the invention, the normal form of cells can be maintained without supplementing serum, rapid proliferation is realized, and the biological characteristics of the mesenchymal stem cells are maintained.

The invention relates to the field of gene engineering, in particular to a daidzein reductase mutant and application of the daidzein reductase mutant in preparation of equol, and the daidzein reductase mutant is prepared by performing one or more of the following mutations on the basis of a wild type: V263F, P107A, H351R, G35L, H171A, A267Q, H351Q, M355Q, H351P, G31V, V176A, H351Y, N64K, V137A, H351W, D63A, W257A and C174N. The catalytic activity of the mutant obtained through precise mutation is remarkably improved under the feedback inhibition condition, especially when high-concentration equol exists, the mutant can still keep high relative activity, and continuity and high efficiency of the biosynthesis process are ensured. When the mutant is applied to biosynthesis of equol, the production titer is remarkably improved, the yield can be improved by about 1.2 times compared with that of a wild type, and the economic benefit and the yield of biosynthesis are directly improved.

The invention provides an external composition for improving the sub-health state of people in a high-glucose lifestyle and application of the external composition, and belongs to the technical field of biological agents. The composition provided by the invention is prepared from the following components: caffeine silanol C, a radix sophorae flavescentis extract, a lotus leaf extract, a corn poppy extract, a bitter gourd extract, soy isoflavone, beta-glucan, a tea leaf extract, an apple extract, resveratrol, a pueraria mirifica extract, a propolis extract, an angelica sinensis root extract, a radix ophiopogonis extract and a rhizoma anemarrhenae root extract. The composition is prepared from a fructus gardeniae extract, a Thermus thermophilus fermentation product, oligopeptide-1 and carnosine. The composition provided by the invention can be prepared into an external preparation, is suitable for people in the high-glucose lifestyle, has the technical effects of improving the sub-health states of the people in the high-glucose lifestyle, such as weakness, skin itch, dark skin color, thirst, frequent urination and the like, and improves the life quality of the people in the high-glucose lifestyle.

A probiotic and prebiotic-embedded synbiotic system for targeted delivery and colonization of functions, comprising a probiotic core; an inner layer of protein-polysaccharide / oligosaccharide complex wrapped around the probiotic surface, which acts as both an adhesion promoter, promoting the attachment of probiotics to the intestinal epithelial cells, and as a prebiotic, promoting the growth of probiotics upon release; and an outer layer of a natural emulsifying system on the synbiotic complex, which minimizes water infiltration, thereby effectively embedding and protecting the probiotics. The present synbiotic system is suitable for production in powder form using freeze-drying technology, and is suitable as a dietary supplement or for addition to any food in dry powder form. The system has the ability to bioconvert soy isoflavones and release bioactive phenolic acids from grains to promote body health.

[Problem] To improve production efficiency in the breeding and farming of crustaceans.　[Solution] Provided are a method for improving the survival rate of crustaceans, a method for producing crustaceans, etc., in which a soybean-isoflavone-containing crustacean feed is fed to crustaceans. In the breeding and farming of crustaceans, a high survival rate during the breeding period can be maintained by feeding a soybean-isoflavone-containing crustacean feed to the crustaceans. Specifically, the number of deaths of crustaceans introduced into breeding and farming can be reduced, and, for example, the number of crustaceans that grow to a level suitable for shipping can be increased; therefore, the production efficiency in the breeding and farming of crustaceans can be improved.

The invention relates to the technical field of traditional Chinese medicines, and discloses a traditional Chinese medicine composition for reducing the content of cholesterol in blood and a preparation method and application thereof. The traditional Chinese medicine composition for reducing the cholesterol content in the blood is prepared from the following raw material medicines in parts by weight: 3-6 parts of black beans, 1-3 parts of vegetables and 0.1-0.3 part of walnuts. The vegetables are selected from spinach or a combination of asparagus and pumpkins; wherein the black beans are black-peel and yellow-kernel varieties, the anthocyanin content is more than or equal to 2.5 mg / g, and the total amount of soy isoflavone is more than or equal to 4.0 mg / g; the spinach is an overwintering variety, and the proportion of soluble dietary fibers is greater than or equal to 16%; according to the traditional Chinese medicine composition for reducing the content of cholesterol in blood, active ingredients such as anthocyanin, isoflavone, unsaturated fatty acid and the like of black beans are fully utilized and have a synergistic effect with dietary fibers and vitamins of specific vegetables, the activity of thermosensitive ingredients is reserved by optimizing a process, the blood cholesterol is effectively reduced, and the traditional Chinese medicine composition is safe, free of side effects, low in cost and suitable for industrial production. The traditional Chinese medicine composition is suitable for prevention or adjuvant treatment of hypercholesteremia and atherosclerosis.

The invention relates to the technical field of microbial fermentation engineering, and particularly discloses natto red yeast rice herb bean flour and a preparation method thereof. The preparation method comprises the following steps: respectively activating specifically preserved bacillus natto and monascus to obtain secondary seed solutions; inoculating the two seed solutions into a solid-state fermentation culture medium for co-fermentation; and inactivating, drying and crushing the fermentation product to obtain a finished product. The solid-state fermentation culture medium is prepared by compounding a bean matrix (soybeans, black beans or chickpeas) and herb tea components (roses, lotus leaves, chrysanthemums, mulberry leaves, haws, cassia seeds and the like). Through the synergistic effect of the bacillus natto and the monascus, nattokinase and monacolin K are efficiently enriched in a single fermentation system at the same time, conversion of soy isoflavone to a high-bioavailability aglycone type is remarkably promoted, and the obtained product has multiple health-care functions of thrombolysis, lipid reduction, efficient absorption and the like.

The invention discloses an oral mucosa absorption gargle tablet based on black cimicifuga foetida extract, and particularly relates to the field of pharmaceutical preparations, the oral mucosa absorption gargle tablet comprises the following components by weight: 30 wt% of a quick release layer and 70 wt% of a slow release layer; the quick release layer is prepared from black cimicifuga foetida nano-liposome, a disintegrating agent and a penetration enhancer, and the mass ratio of the black cimicifuga foetida nano-liposome to the disintegrating agent to the penetration enhancer in the quick release layer is 4: 1: 1; the slow-release layer is prepared from chitosan, soy isoflavone, a pH sensitive material and a flavoring agent, and the mass ratio of the chitosan to the soy isoflavone to the pH sensitive material to the flavoring agent in the slow-release layer is 2: 3: 1: 2. Through the double-layer structure design of the quick release layer and the slow release layer, quick effect and continuous effect of active ingredients are realized, and the oral preparation is obviously superior to a traditional oral preparation; the black cimicifuga foetida nano-liposome and the menthol-beta-cyclodextrin inclusion compound have a synergistic effect, so that the cumulative infiltration capacity of mucous membranes within 24 hours reaches 82.5%, and the transmembrane absorption efficiency of active ingredients is greatly improved.

The invention relates to the field of blood-enriching oral liquid, in particular to Zhenqi blood-enriching oral liquid. Sheep placentas, pearls, red ginseng, Chinese wolfberry fruits, astragalus membranaceus, radix angelicae, coix seeds, fresh ginger, Chinese dates, black beans, angelica sinensis, radix puerariae and Chinese herbaceous peony are used as functional component raw materials. The sheep placenta is hydrolyzed by pancreatin to obtain hydrolysate rich in protein, polypeptide, amino acid, collagen, female progestational hormone and the like, and the palatability of the oral liquid is improved through deodorization and decoloration treatment of activated carbon. Neutral protease is firstly added into pearls for enzymolysis, then hydrochloric acid is added for hydrolysis, and protein is converted into a water-soluble component with smaller molecular weight. The pearl hydrolysate is good in water solubility, high in absorptivity and rich in active substances. Soybean isoflavone contained in the black beans is similar to estrogen secreted by a human body in structure. Paeonia lactiflora has the functions of dredging channels and promoting blood circulation. And the other nine blood-enriching traditional Chinese medicines are mutually coordinated to achieve the purposes of nourishing yin and blood, enriching blood and soothing nerves. Qi and blood are effectively regulated, and hypomenorrhea and thin menstruation are treated.

The invention discloses a quantitative trait locus related to soybean isoflavone content and an application thereof, and provides a soybean molecular marker-assisted breeding method based on a high-density genetic linkage map. A cultivated soybean variety JD12ZDD23040 is selected to be hybridized with a wild soybean variety Y9ZYD02739, and a control group consisting of 185 recombinant inbred lines (RILs) from F8 sources is constructed by a single seed descent SSD method; genotyping is performed by sequencing and SNP calling, and map construction, QTL detection and QTL integration analysis are performed; based on statistical analysis, an integrated QTL significantly related to the soybean isoflavone content trait is obtained; based on the obtained integrated QTL, a molecular marker and a primer thereof are developed for soybean molecular marker-assisted breeding related to the soybean isoflavone content trait.

The invention relates to a food uric acid reducing composition. The food uric acid reducing composition comprises main materials and auxiliary materials, the mass ratio of the main material to the auxiliary material is 5: 1; the main material is prepared from the following raw materials in parts by weight: 10 to 15 parts of frankincense powder, 10 to 15 parts of tuna peptide, 50 to 100 parts of freeze-dried mulberry powder, 50 to 100 parts of soy isoflavone, 30 to 50 parts of red yeast rice powder, 10 to 20 parts of turmeric powder, 40 to 80 parts of marine red algae powder and 100 to 200 parts of celery seed powder; the auxiliary materials comprise at least one of the following auxiliary materials: a first auxiliary material which is selected from at least one of chicory powder, poria cocos powder and B vitamins; the second auxiliary material is selected from at least one of radix puerariae powder and lophatherum gracile powder; and the third auxiliary material is selected from at least one of sour cherry powder and licorice powder. According to the food uric acid reducing composition, the uric acid reducing effect is improved through combination of specific raw materials, the taste and taste of the product can be improved through food-borne materials, side effects are reduced, and the food uric acid reducing composition is suitable for being taken for a long time.

The invention provides a pulping control method and device, a storage medium and a food processer, the method is applied to the cooking field, and the method comprises the following steps: controlling a heating device, a stirring device or a ventilation device of the food processer to work according to different control parameters in different cooking stages in the pulping process of the food processer, oxygen-containing gas is introduced into the water-material mixture in the cooking cavity through the ventilation device, so that glucoside-type soy isoflavone released in the water-material mixture generates free isoflavone under the action of endogenous enzyme, and the nutritional value of the finally prepared slurry is improved; meanwhile, the Maillard reaction of protein and reducing sugar in the water-material mixture can be promoted, and the aroma and taste of the finally prepared pulp are improved.

The utility model relates to the field of extraction equipment, in particular to an extraction device for producing soy isoflavone, which comprises an extraction box, a cover plate and the like, the extraction box is an extraction main body of the device, the top of the extraction box is a feeding hole for adding raw materials, the cover plate is mounted at the feeding hole in the top of the extraction box, and handles are symmetrically mounted on the left side and the right side of the cover plate; the electric push rods I are symmetrically mounted on two sides of the upper part of the extraction box; and the lock hole blocks are symmetrically and fixedly connected to the two sides of the extraction box. According to the utility model, the quantitative block controlled by the electric push rod II is matched with the guide frame, so that accurate quantitative filling of a solvent can be realized, the consistency of the amount of the solvent added every time is ensured, the standardization of production is facilitated, meanwhile, the scale strip arranged on the inner wall of the extraction box is beneficial to accurately measuring the amount of the added soybean raw material, errors caused by manual estimation are reduced, and the production efficiency is improved. The effect of quantitatively controlling the raw material and solvent filling proportion is achieved.

This invention discloses the application of plant extracts in the preparation of drugs that inhibit canine parvovirus, belonging to the field of biomedical technology. The plant extract is at least one selected from phlorizin, neomethylhesperidin-dihydrochalcone, tribulus terrestris saponin, stevia glycoside, soy isoflavones, berberine hydrochloride, diosgenin, and silymarin. Through in-depth research on various plant extracts, this invention has found that phlorizin, neomethylhesperidin-dihydrochalcone, tribulus terrestris saponin, stevia glycoside, soy isoflavones, berberine hydrochloride, diosgenin, and silymarin all exhibit significant inhibitory effects on canine parvovirus, with soy isoflavones showing particularly outstanding effects. This research provides a novel natural drug option for the prevention and treatment of canine parvovirus. This invention not only expands the application scope of natural plant extracts in the veterinary medicine field but also provides important technical support for the development of novel, highly effective, safe, and side-effect-free drug formulations for the prevention and treatment of canine parvovirus.

This invention discloses an S-estrol composition for delaying ovarian aging and its uses, relating to the fields of biomedicine and health products. The S-estrol composition for delaying ovarian aging comprises, by weight, the following components: 1-5 parts S-estrol, 2-8 parts soy isoflavones, 3-10 parts coenzyme Q10, 2-6 parts vitamin E, 4-12 parts vitamin C, 0.01-0.05 parts folic acid, 0.1-0.3 parts zinc source, 0.5-1.5 parts anti-aging protective agent, 50-80 parts carrier, and 10-15 parts purified water. This invention uses high-purity S-estrol as the core, combined with soy isoflavones, coenzyme Q10 and a variety of antioxidant nutrients in a scientific formulation to form a synergistic system for estrogen receptor signal regulation and antioxidant defense. By constructing a complex antioxidant network and introducing novel anti-aging protectants for synergistic effect, the activity of antioxidant enzymes tends to increase and the level of lipid peroxidation tends to decrease, thereby improving the oxidative stress state in ovarian cells as a whole.

The invention relates to the technical field of food processing, in particular to a method for supercritical carbon dioxide extraction of glycine soja isoflavone and a product. The method comprises the following steps: taking 30-40-mesh defatted soybean meal, putting the defatted soybean meal into an extraction kettle, putting 70% ethanol into an entrainer pump, and carrying out supercritical carbon dioxide extraction under the conditions that the extraction temperature is 50 DEG C, the extraction pressure is 35 mpa, the extraction time is 2 hours, the separation I temperature is 32 DEG C, the separation I pressure is 6 mpa, and the separation II temperature is 30 DEG C and the separation II pressure is 5 mpa; an extracted substance is yellow green liquid, and solanum nigrum isoflavone solid powder can be obtained through subsequent rotary evaporation and freeze-drying treatment. By exploring the interactive influence of the material-liquid ratio, the extraction pressure, the extraction temperature, the extraction time, the particle size and different extraction conditions on the extraction amount of the soybean isoflavone, the method for extracting the soybean isoflavone through the supercritical carbon dioxide is provided, and the extraction amount of the soybean isoflavone can be remarkably increased.

The invention relates to a liquid microbial enzyme as well as a preparation method and application thereof. The liquid microbial enzyme is prepared by taking soybean meal, fish meal, rice husk and water as raw materials, sterilizing and fermenting through a compound microbial agent, and the compound microbial agent comprises bacillus subtilis, bacillus licheniformis and bacillus velezensis. In the application, the soybean meal serves as a core nitrogen source and an energy source, soybean isoflavone of the soybean meal promotes functional bacteria to proliferate and enhances enzyme activity, and a fiber structure can optimize fermentation conditions; the fish meal provides high-quality protein which can be decomposed to generate organic acid, so that the pH value is reduced, and putrefying bacteria are inhibited; the rice husks maintain the material structure, and the porous characteristic improves ventilation and adsorbs peculiar smell; the bacillus velezensis as an auxiliary enzyme source can enhance the variety coverage of soluble enzymes, so that the substrate degradation capacity is more comprehensive. After the liquid microbial enzyme is prepared, the liquid microbial enzyme and the hermetia illucens are combined, livestock and poultry manure is used as a conversion object, and the conversion efficiency of the livestock and poultry manure on the manure is improved by regulating and controlling intestinal microflora of larvae of the hermetia illucens.

The invention relates to a feed additive for optimizing nutritional ingredients of poultry eggs. The feed additive comprises natural grains, soybean flavone, lotus leaves, microalgae and a microalgae extract. The feed additive comprises the following raw materials in percentage by weight: 20-70% of corn, 5-10% of wheat bran, 5-10% of soybean meal, 2-5% of soybean isoflavone, 5-10% of lotus leaf powder, 10-20% of spirulina powder, 5-10% of chlorella powder and 5-20% of haematococcus pluvialis. The preparation method comprises the following steps: fully stirring and uniformly mixing the components, sub-packaging, adding into laying fowl feed according to the proportion of 1-2wt%, fully and uniformly mixing, feeding laying hens, and domesticating and feeding for 2-3 weeks to obtain eggs rich in nutritional ingredients such as proteins, flavonoid compounds, algae source carotenoids, vitamins, inorganic salts and the like. Compared with common eggs, the egg yolk cholesterol content can be reduced by more than 1 / 4; the egg white and the yolk are clear in boundary, the yolk is orange red, the fishy smell is obviously smaller than that of common eggs, and the yolk tastes fine and smooth; the obtained feed additive has a remarkable optimization effect on nutritional ingredients of poultry eggs, meanwhile, the taste and quality are improved, and economic benefits are increased.

The present application relates to the technical field of traditional Chinese medicine composition, and discloses a traditional Chinese medicine composition for relieving rheumatoid arthritis and a preparation method thereof, which comprises Qingfengteng, Huangqi, Chuanshanyang, propolis, turmeric, Fuling, soybean isoflavone peptide, chitosan, Gancao, Chufu, Guizhi and Dangui, and the preparation method comprises raw material pretreatment, extraction, active ingredient mixing and preparation forming. The traditional Chinese medicine composition prepared by the present application has a maximum inhibition rate of 88.6% and 86.4% on TNF-alpha and IL-6, a bioavailability of 87.5%, and can improve immune function and quickly relieve joint swelling and pain. Moreover, the preparation process of the composition is stable and suitable for industrial production, and the composition has significant clinical application value in the treatment of rheumatoid arthritis.

The invention discloses a method for producing high-activity soybean isoflavone by fermenting soybean milk through lactobacillus pentosus and a solid beverage prepared through the method, and relates to the technical field of microbial fermention.The method comprises the following steps that the soybean milk is inoculated with the lactobacillus pentosus 068-1, fermentation is conducted for 36-60 h at the temperature of 32-40 DEG C, and the high-activity soybean isoflavone is obtained. After fermentation with lactobacillus pentosus, the soybean isoflavone is hydrolyzed from combined soybean isoflavone to free soybean isoflavone, the fermentation process is simple and easy to control, and the method has the advantage of high repeatability of microbial fermentation, is not influenced by territory and environmental factors, and has a good hydrolysis effect.

This invention provides a composition containing soy isoflavones, its preparation method, and its application, relating to the technical field of cosmetics. The soy isoflavone-containing composition includes soy isoflavones, an oil phase matrix, liposome excipients, a transdermal absorption enhancer, a moisturizer, an emulsifier, an antioxidant, a chelating agent, and a preservative; wherein the liposome excipients include a first liposome excipient serving as a membrane framework and a second liposome excipient serving as a membrane modulator. The composition of this invention significantly improves the transdermal absorption efficiency of soy isoflavones in cosmetics, achieving a synergistic effect of multiple functions such as oil control and acne treatment, moisturizing and repairing, brightening, and anti-aging; simultaneously, it further reduces skin irritation, improving the safety and effectiveness of the product in people with sensitive and acne-prone skin.

The invention relates to the technical field of biological medicine and health care, in particular to a production method and formula for balancing female hormone level, and the production method comprises the following steps: mixing plant extract, microbial metabolite and functional lipid to form a basic active mixture, adding a trace element compound, and performing microencapsulation treatment. The composition comprises an evening primrose oil extract, a soy isoflavone extract and the like, and accurate regulation and control are realized by regulating a hormone synthesis route and signal transduction. The invention further provides an application method and an effect evaluation mode, the formula proportion can be adjusted according to individual hormone detection results, and the composition is suggested to be taken after breakfast so as to improve the absorption efficiency. The personalized health management requirement can be efficiently and safely met, and meanwhile the long-term effect stability is improved.

The invention provides a pulping control method and device, a storage medium and a food processer, the method is applied to the cooking field, and the method comprises the steps that a stirring device and a heating device of the food processer are controlled to work according to different control parameters in different cooking stages in the pulping process of the food processer, introducing air flow into a cooking cavity of the food processor in at least one of the first cooking stage and the third cooking stage so as to introduce oxygen-containing gas into the water-material mixture in the cooking cavity, so that glucoside-type soy isoflavone released in the water-material mixture generates free isoflavone under the action of endogenous enzyme; according to the present invention, the nutritional value of the finally prepared slurry is improved, the rich protein, the reducing sugar and other sweet substances are released during the first cooking stage and the second cooking stage, the Maillard reaction of the protein and the reducing sugar in the water-material mixture can be promoted, and the aroma and the sweet taste of the finally prepared slurry are improved.

The application discloses a kind of compound enzyme and its application, belong to the field of biotechnology.The compound enzyme provided by the application includes: pectinase 5-15 parts by weight, glucose glycosidase 5-15 parts, glycerol 2-8 parts and water 15-20 parts, the compound enzyme also includes phytase 2-6 parts, lactic acid dehydrogenase 1-4 parts.The compound enzyme provided by the application can significantly improve the extraction rate of soybean isoflavones, and the content of isoflavones is significantly improved.

The invention discloses a method for purifying S-equol in genetic engineering escherichia coli fermentation liquor. According to the method, daidzein is used as a fermentation substrate, and S-equol-producing genetic engineering escherichia coli is used for fermenting escherichia coli BL21-G2 to obtain fermentation liquor; then, according to the characteristic of high polarity of the S-equol, extracting the S-equol generated by fermentation from the liquid phase by using ethyl acetate; and finally, separating and purifying S-equol and other soy isoflavones by using a specific ratio of chloroform and methanol chromatography liquid through a silica gel column, further separating and purifying S-equol and other impurities in the fermentation liquid through an ethyl acetate chromatography liquid, and finally obtaining S-equol with the purity close to 100%. Compared with a traditional ethanol column-passing extraction method, a one-step silica gel column-passing separation method and the like, the method is economical, practical and good in effect, the purity of the obtained S-equol is higher, and the process research prospect is wide.