86 results about "SOY ISOFLAVONES" patented technology

The invention belongs to the field of agriculture, and discloses an extraction method of soybean isoflavone, which comprises the following steps: taking soybean straws as raw materials, and carrying out ultrasonic-assisted alcohol extraction to obtain soybean isoflavone; the extraction rate of the soy isoflavone is not lower than 6mg / g. By means of the method, a considerable amount of soybean isoflavone can be extracted from the soybean straw, and the cognition on the extraction rate of the soybean straw and the soybean isoflavone in the prior art is far better than the cognition on the extraction rate of the soybean straw and the soybean isoflavone. Meanwhile, the invention further provides new application of the soybean straw.

The invention relates to the technical field of cereal milk tea preparation, in particular to sea-buckthorn cereal milk tea and a preparation method thereof.The method comprises the steps that sodium caseinate is stirred and dissolved in deionized water and then subjected to water bath to obtain a sodium caseinate solution, and soy isoflavone is fused into the cooled sodium caseinate solution to obtain a film forming solution; performing vacuum spray drying on the hippophae rhamnoides fruit solution through a vacuum spray dryer to obtain hippophae rhamnoides fruit powder particles, and mixing the film-formed fried rice, the hippophae rhamnoides fruit powder particles and ingredients to obtain the hippophae rhamnoides fruit grain milk tea, determining whether the preparation stability of the hippophae rhamnoides fruit grain milk tea meets the requirement or not based on the maximum difference quantity of the thickness of the film layer on the surface of the film-formed fried rice; determining whether the immersion depth of the stirring blades needs to be increased or not; determining whether the inclination angle of the drum needs to be reduced; and determining the number of cyclic shearing times based on the volume average particle size of the sea buckthorn fruit powder particles. The preparation stability of the hippophae rhamnoides fruit grain milk tea is improved.

The invention relates to the technical field of daily chemical products, in particular to a composition with a tightening effect and application of the composition in the daily chemical products. The composition is prepared from the following raw materials: a vine calli extracting solution, a caramel pedicel extracting solution, a coconut tomato extract, soy isoflavone, a bacillus fermentation product, an aloe barbadensis extract, a centella asiatica leaf extract, hamamelis virginiana water, a radix sophorae flavescentis extract, a fructus cnidii extract, a folium artemisiae argyi extract, a chamomile extract and a rose flower extract. And a scutellaria baicalensis root extract and a solubilizer. The composition disclosed by the invention adopts various natural plant extracts, the safety is high, various combinations are matched with one another, a synergistic tightening effect is achieved, and the added tenuikalii extracting solution and kacip flower pedicel maca extracting solution have remarkable tightening and beautifying effects.

The invention relates to the field of gene engineering, in particular to a daidzein reductase mutant and application of the daidzein reductase mutant in preparation of equol, and the daidzein reductase mutant is prepared by performing one or more of the following mutations on the basis of a wild type: V263F, P107A, H351R, G35L, H171A, A267Q, H351Q, M355Q, H351P, G31V, V176A, H351Y, N64K, V137A, H351W, D63A, W257A and C174N. The catalytic activity of the mutant obtained through precise mutation is remarkably improved under the feedback inhibition condition, especially when high-concentration equol exists, the mutant can still keep high relative activity, and continuity and high efficiency of the biosynthesis process are ensured. When the mutant is applied to biosynthesis of equol, the production titer is remarkably improved, the yield can be improved by about 1.2 times compared with that of a wild type, and the economic benefit and the yield of biosynthesis are directly improved.

The invention provides an external composition for improving the sub-health state of people in a high-glucose lifestyle and application of the external composition, and belongs to the technical field of biological agents. The composition provided by the invention is prepared from the following components: caffeine silanol C, a radix sophorae flavescentis extract, a lotus leaf extract, a corn poppy extract, a bitter gourd extract, soy isoflavone, beta-glucan, a tea leaf extract, an apple extract, resveratrol, a pueraria mirifica extract, a propolis extract, an angelica sinensis root extract, a radix ophiopogonis extract and a rhizoma anemarrhenae root extract. The composition is prepared from a fructus gardeniae extract, a Thermus thermophilus fermentation product, oligopeptide-1 and carnosine. The composition provided by the invention can be prepared into an external preparation, is suitable for people in the high-glucose lifestyle, has the technical effects of improving the sub-health states of the people in the high-glucose lifestyle, such as weakness, skin itch, dark skin color, thirst, frequent urination and the like, and improves the life quality of the people in the high-glucose lifestyle.

A probiotic and prebiotic-embedded synbiotic system for targeted delivery and colonization of functions, comprising a probiotic core; an inner layer of protein-polysaccharide / oligosaccharide complex wrapped around the probiotic surface, which acts as both an adhesion promoter, promoting the attachment of probiotics to the intestinal epithelial cells, and as a prebiotic, promoting the growth of probiotics upon release; and an outer layer of a natural emulsifying system on the synbiotic complex, which minimizes water infiltration, thereby effectively embedding and protecting the probiotics. The present synbiotic system is suitable for production in powder form using freeze-drying technology, and is suitable as a dietary supplement or for addition to any food in dry powder form. The system has the ability to bioconvert soy isoflavones and release bioactive phenolic acids from grains to promote body health.

[Problem] To improve production efficiency in the breeding and farming of crustaceans.　[Solution] Provided are a method for improving the survival rate of crustaceans, a method for producing crustaceans, etc., in which a soybean-isoflavone-containing crustacean feed is fed to crustaceans. In the breeding and farming of crustaceans, a high survival rate during the breeding period can be maintained by feeding a soybean-isoflavone-containing crustacean feed to the crustaceans. Specifically, the number of deaths of crustaceans introduced into breeding and farming can be reduced, and, for example, the number of crustaceans that grow to a level suitable for shipping can be increased; therefore, the production efficiency in the breeding and farming of crustaceans can be improved.

The invention relates to the technical field of traditional Chinese medicines, and discloses a traditional Chinese medicine composition for reducing the content of cholesterol in blood and a preparation method and application thereof. The traditional Chinese medicine composition for reducing the cholesterol content in the blood is prepared from the following raw material medicines in parts by weight: 3-6 parts of black beans, 1-3 parts of vegetables and 0.1-0.3 part of walnuts. The vegetables are selected from spinach or a combination of asparagus and pumpkins; wherein the black beans are black-peel and yellow-kernel varieties, the anthocyanin content is more than or equal to 2.5 mg / g, and the total amount of soy isoflavone is more than or equal to 4.0 mg / g; the spinach is an overwintering variety, and the proportion of soluble dietary fibers is greater than or equal to 16%; according to the traditional Chinese medicine composition for reducing the content of cholesterol in blood, active ingredients such as anthocyanin, isoflavone, unsaturated fatty acid and the like of black beans are fully utilized and have a synergistic effect with dietary fibers and vitamins of specific vegetables, the activity of thermosensitive ingredients is reserved by optimizing a process, the blood cholesterol is effectively reduced, and the traditional Chinese medicine composition is safe, free of side effects, low in cost and suitable for industrial production. The traditional Chinese medicine composition is suitable for prevention or adjuvant treatment of hypercholesteremia and atherosclerosis.

The invention relates to the technical field of microbial fermentation engineering, and particularly discloses natto red yeast rice herb bean flour and a preparation method thereof. The preparation method comprises the following steps: respectively activating specifically preserved bacillus natto and monascus to obtain secondary seed solutions; inoculating the two seed solutions into a solid-state fermentation culture medium for co-fermentation; and inactivating, drying and crushing the fermentation product to obtain a finished product. The solid-state fermentation culture medium is prepared by compounding a bean matrix (soybeans, black beans or chickpeas) and herb tea components (roses, lotus leaves, chrysanthemums, mulberry leaves, haws, cassia seeds and the like). Through the synergistic effect of the bacillus natto and the monascus, nattokinase and monacolin K are efficiently enriched in a single fermentation system at the same time, conversion of soy isoflavone to a high-bioavailability aglycone type is remarkably promoted, and the obtained product has multiple health-care functions of thrombolysis, lipid reduction, efficient absorption and the like.

The invention discloses an oral mucosa absorption gargle tablet based on black cimicifuga foetida extract, and particularly relates to the field of pharmaceutical preparations, the oral mucosa absorption gargle tablet comprises the following components by weight: 30 wt% of a quick release layer and 70 wt% of a slow release layer; the quick release layer is prepared from black cimicifuga foetida nano-liposome, a disintegrating agent and a penetration enhancer, and the mass ratio of the black cimicifuga foetida nano-liposome to the disintegrating agent to the penetration enhancer in the quick release layer is 4: 1: 1; the slow-release layer is prepared from chitosan, soy isoflavone, a pH sensitive material and a flavoring agent, and the mass ratio of the chitosan to the soy isoflavone to the pH sensitive material to the flavoring agent in the slow-release layer is 2: 3: 1: 2. Through the double-layer structure design of the quick release layer and the slow release layer, quick effect and continuous effect of active ingredients are realized, and the oral preparation is obviously superior to a traditional oral preparation; the black cimicifuga foetida nano-liposome and the menthol-beta-cyclodextrin inclusion compound have a synergistic effect, so that the cumulative infiltration capacity of mucous membranes within 24 hours reaches 82.5%, and the transmembrane absorption efficiency of active ingredients is greatly improved.

The invention relates to the field of blood-enriching oral liquid, in particular to Zhenqi blood-enriching oral liquid. Sheep placentas, pearls, red ginseng, Chinese wolfberry fruits, astragalus membranaceus, radix angelicae, coix seeds, fresh ginger, Chinese dates, black beans, angelica sinensis, radix puerariae and Chinese herbaceous peony are used as functional component raw materials. The sheep placenta is hydrolyzed by pancreatin to obtain hydrolysate rich in protein, polypeptide, amino acid, collagen, female progestational hormone and the like, and the palatability of the oral liquid is improved through deodorization and decoloration treatment of activated carbon. Neutral protease is firstly added into pearls for enzymolysis, then hydrochloric acid is added for hydrolysis, and protein is converted into a water-soluble component with smaller molecular weight. The pearl hydrolysate is good in water solubility, high in absorptivity and rich in active substances. Soybean isoflavone contained in the black beans is similar to estrogen secreted by a human body in structure. Paeonia lactiflora has the functions of dredging channels and promoting blood circulation. And the other nine blood-enriching traditional Chinese medicines are mutually coordinated to achieve the purposes of nourishing yin and blood, enriching blood and soothing nerves. Qi and blood are effectively regulated, and hypomenorrhea and thin menstruation are treated.

The invention provides a pulping control method and device, a storage medium and a food processer, the method is applied to the cooking field, and the method comprises the following steps: controlling a heating device, a stirring device or a ventilation device of the food processer to work according to different control parameters in different cooking stages in the pulping process of the food processer, oxygen-containing gas is introduced into the water-material mixture in the cooking cavity through the ventilation device, so that glucoside-type soy isoflavone released in the water-material mixture generates free isoflavone under the action of endogenous enzyme, and the nutritional value of the finally prepared slurry is improved; meanwhile, the Maillard reaction of protein and reducing sugar in the water-material mixture can be promoted, and the aroma and taste of the finally prepared pulp are improved.

The utility model relates to the field of extraction equipment, in particular to an extraction device for producing soy isoflavone, which comprises an extraction box, a cover plate and the like, the extraction box is an extraction main body of the device, the top of the extraction box is a feeding hole for adding raw materials, the cover plate is mounted at the feeding hole in the top of the extraction box, and handles are symmetrically mounted on the left side and the right side of the cover plate; the electric push rods I are symmetrically mounted on two sides of the upper part of the extraction box; and the lock hole blocks are symmetrically and fixedly connected to the two sides of the extraction box. According to the utility model, the quantitative block controlled by the electric push rod II is matched with the guide frame, so that accurate quantitative filling of a solvent can be realized, the consistency of the amount of the solvent added every time is ensured, the standardization of production is facilitated, meanwhile, the scale strip arranged on the inner wall of the extraction box is beneficial to accurately measuring the amount of the added soybean raw material, errors caused by manual estimation are reduced, and the production efficiency is improved. The effect of quantitatively controlling the raw material and solvent filling proportion is achieved.

This invention discloses the application of plant extracts in the preparation of drugs that inhibit canine parvovirus, belonging to the field of biomedical technology. The plant extract is at least one selected from phlorizin, neomethylhesperidin-dihydrochalcone, tribulus terrestris saponin, stevia glycoside, soy isoflavones, berberine hydrochloride, diosgenin, and silymarin. Through in-depth research on various plant extracts, this invention has found that phlorizin, neomethylhesperidin-dihydrochalcone, tribulus terrestris saponin, stevia glycoside, soy isoflavones, berberine hydrochloride, diosgenin, and silymarin all exhibit significant inhibitory effects on canine parvovirus, with soy isoflavones showing particularly outstanding effects. This research provides a novel natural drug option for the prevention and treatment of canine parvovirus. This invention not only expands the application scope of natural plant extracts in the veterinary medicine field but also provides important technical support for the development of novel, highly effective, safe, and side-effect-free drug formulations for the prevention and treatment of canine parvovirus.

This invention discloses an S-estrol composition for delaying ovarian aging and its uses, relating to the fields of biomedicine and health products. The S-estrol composition for delaying ovarian aging comprises, by weight, the following components: 1-5 parts S-estrol, 2-8 parts soy isoflavones, 3-10 parts coenzyme Q10, 2-6 parts vitamin E, 4-12 parts vitamin C, 0.01-0.05 parts folic acid, 0.1-0.3 parts zinc source, 0.5-1.5 parts anti-aging protective agent, 50-80 parts carrier, and 10-15 parts purified water. This invention uses high-purity S-estrol as the core, combined with soy isoflavones, coenzyme Q10 and a variety of antioxidant nutrients in a scientific formulation to form a synergistic system for estrogen receptor signal regulation and antioxidant defense. By constructing a complex antioxidant network and introducing novel anti-aging protectants for synergistic effect, the activity of antioxidant enzymes tends to increase and the level of lipid peroxidation tends to decrease, thereby improving the oxidative stress state in ovarian cells as a whole.

The invention relates to the technical field of food processing, in particular to a method for supercritical carbon dioxide extraction of glycine soja isoflavone and a product. The method comprises the following steps: taking 30-40-mesh defatted soybean meal, putting the defatted soybean meal into an extraction kettle, putting 70% ethanol into an entrainer pump, and carrying out supercritical carbon dioxide extraction under the conditions that the extraction temperature is 50 DEG C, the extraction pressure is 35 mpa, the extraction time is 2 hours, the separation I temperature is 32 DEG C, the separation I pressure is 6 mpa, and the separation II temperature is 30 DEG C and the separation II pressure is 5 mpa; an extracted substance is yellow green liquid, and solanum nigrum isoflavone solid powder can be obtained through subsequent rotary evaporation and freeze-drying treatment. By exploring the interactive influence of the material-liquid ratio, the extraction pressure, the extraction temperature, the extraction time, the particle size and different extraction conditions on the extraction amount of the soybean isoflavone, the method for extracting the soybean isoflavone through the supercritical carbon dioxide is provided, and the extraction amount of the soybean isoflavone can be remarkably increased.

The invention relates to a liquid microbial enzyme as well as a preparation method and application thereof. The liquid microbial enzyme is prepared by taking soybean meal, fish meal, rice husk and water as raw materials, sterilizing and fermenting through a compound microbial agent, and the compound microbial agent comprises bacillus subtilis, bacillus licheniformis and bacillus velezensis. In the application, the soybean meal serves as a core nitrogen source and an energy source, soybean isoflavone of the soybean meal promotes functional bacteria to proliferate and enhances enzyme activity, and a fiber structure can optimize fermentation conditions; the fish meal provides high-quality protein which can be decomposed to generate organic acid, so that the pH value is reduced, and putrefying bacteria are inhibited; the rice husks maintain the material structure, and the porous characteristic improves ventilation and adsorbs peculiar smell; the bacillus velezensis as an auxiliary enzyme source can enhance the variety coverage of soluble enzymes, so that the substrate degradation capacity is more comprehensive. After the liquid microbial enzyme is prepared, the liquid microbial enzyme and the hermetia illucens are combined, livestock and poultry manure is used as a conversion object, and the conversion efficiency of the livestock and poultry manure on the manure is improved by regulating and controlling intestinal microflora of larvae of the hermetia illucens.

The present application relates to the technical field of traditional Chinese medicine composition, and discloses a traditional Chinese medicine composition for relieving rheumatoid arthritis and a preparation method thereof, which comprises Qingfengteng, Huangqi, Chuanshanyang, propolis, turmeric, Fuling, soybean isoflavone peptide, chitosan, Gancao, Chufu, Guizhi and Dangui, and the preparation method comprises raw material pretreatment, extraction, active ingredient mixing and preparation forming. The traditional Chinese medicine composition prepared by the present application has a maximum inhibition rate of 88.6% and 86.4% on TNF-alpha and IL-6, a bioavailability of 87.5%, and can improve immune function and quickly relieve joint swelling and pain. Moreover, the preparation process of the composition is stable and suitable for industrial production, and the composition has significant clinical application value in the treatment of rheumatoid arthritis.

The invention discloses a method for producing high-activity soybean isoflavone by fermenting soybean milk through lactobacillus pentosus and a solid beverage prepared through the method, and relates to the technical field of microbial fermention.The method comprises the following steps that the soybean milk is inoculated with the lactobacillus pentosus 068-1, fermentation is conducted for 36-60 h at the temperature of 32-40 DEG C, and the high-activity soybean isoflavone is obtained. After fermentation with lactobacillus pentosus, the soybean isoflavone is hydrolyzed from combined soybean isoflavone to free soybean isoflavone, the fermentation process is simple and easy to control, and the method has the advantage of high repeatability of microbial fermentation, is not influenced by territory and environmental factors, and has a good hydrolysis effect.

This invention provides a composition containing soy isoflavones, its preparation method, and its application, relating to the technical field of cosmetics. The soy isoflavone-containing composition includes soy isoflavones, an oil phase matrix, liposome excipients, a transdermal absorption enhancer, a moisturizer, an emulsifier, an antioxidant, a chelating agent, and a preservative; wherein the liposome excipients include a first liposome excipient serving as a membrane framework and a second liposome excipient serving as a membrane modulator. The composition of this invention significantly improves the transdermal absorption efficiency of soy isoflavones in cosmetics, achieving a synergistic effect of multiple functions such as oil control and acne treatment, moisturizing and repairing, brightening, and anti-aging; simultaneously, it further reduces skin irritation, improving the safety and effectiveness of the product in people with sensitive and acne-prone skin.

The invention relates to the technical field of biological medicine and health care, in particular to a production method and formula for balancing female hormone level, and the production method comprises the following steps: mixing plant extract, microbial metabolite and functional lipid to form a basic active mixture, adding a trace element compound, and performing microencapsulation treatment. The composition comprises an evening primrose oil extract, a soy isoflavone extract and the like, and accurate regulation and control are realized by regulating a hormone synthesis route and signal transduction. The invention further provides an application method and an effect evaluation mode, the formula proportion can be adjusted according to individual hormone detection results, and the composition is suggested to be taken after breakfast so as to improve the absorption efficiency. The personalized health management requirement can be efficiently and safely met, and meanwhile the long-term effect stability is improved.

The invention provides a pulping control method and device, a storage medium and a food processer, the method is applied to the cooking field, and the method comprises the steps that a stirring device and a heating device of the food processer are controlled to work according to different control parameters in different cooking stages in the pulping process of the food processer, introducing air flow into a cooking cavity of the food processor in at least one of the first cooking stage and the third cooking stage so as to introduce oxygen-containing gas into the water-material mixture in the cooking cavity, so that glucoside-type soy isoflavone released in the water-material mixture generates free isoflavone under the action of endogenous enzyme; according to the present invention, the nutritional value of the finally prepared slurry is improved, the rich protein, the reducing sugar and other sweet substances are released during the first cooking stage and the second cooking stage, the Maillard reaction of the protein and the reducing sugar in the water-material mixture can be promoted, and the aroma and the sweet taste of the finally prepared slurry are improved.

The application discloses a kind of compound enzyme and its application, belong to the field of biotechnology.The compound enzyme provided by the application includes: pectinase 5-15 parts by weight, glucose glycosidase 5-15 parts, glycerol 2-8 parts and water 15-20 parts, the compound enzyme also includes phytase 2-6 parts, lactic acid dehydrogenase 1-4 parts.The compound enzyme provided by the application can significantly improve the extraction rate of soybean isoflavones, and the content of isoflavones is significantly improved.

The invention discloses a method for purifying S-equol in genetic engineering escherichia coli fermentation liquor. According to the method, daidzein is used as a fermentation substrate, and S-equol-producing genetic engineering escherichia coli is used for fermenting escherichia coli BL21-G2 to obtain fermentation liquor; then, according to the characteristic of high polarity of the S-equol, extracting the S-equol generated by fermentation from the liquid phase by using ethyl acetate; and finally, separating and purifying S-equol and other soy isoflavones by using a specific ratio of chloroform and methanol chromatography liquid through a silica gel column, further separating and purifying S-equol and other impurities in the fermentation liquid through an ethyl acetate chromatography liquid, and finally obtaining S-equol with the purity close to 100%. Compared with a traditional ethanol column-passing extraction method, a one-step silica gel column-passing separation method and the like, the method is economical, practical and good in effect, the purity of the obtained S-equol is higher, and the process research prospect is wide.

The utility model relates to soybean isoflavone processing technical field especially relates to a soybean isoflavone raw material screening and cleaning device, it includes the soybean cleaning cylinder for soybean isoflavone raw material screening, and the inside of soybean cleaning cylinder is provided with screening and cleaning subassembly, screening and cleaning subassembly includes hollow shaft, and the inside of soybean cleaning cylinder is rotatably installed in hollow shaft, and the lower pipe and the upper pipe are installed in the circumferential equidistance communication of hollow shaft lateral wall, the bottom of lower pipe and the upper side of upper pipe all are screw -jointed installation a plurality of stirring vertical pipes, and a plurality of gas holes are seted up on the lateral wall of stirring vertical pipe, and the side of soybean cleaning cylinder is provided with air pump, and the output end of air pump is connected and installed with gas pipe, and the lower end of gas pipe is fixedly installed with sealing sleeve, and the upper end of hollow shaft is rotatably connected with sealing sleeve, the utility model discloses through setting screening and cleaning subassembly, adopt the comprehensive impurity removal mode of soaking, stirring and pneumatic suspension, avoid the drawback of traditional filter screen blockage, improve the cleaning efficiency of soybean.

The invention discloses sea cucumber peptide health-care food with liver protection activity and a preparation method thereof, and belongs to the technical field of health-care food. The health-care food comprises the following raw materials in parts by weight: 80-90 parts of sea cucumber peptide, 5-12 parts of a Chinese magnoliavine fruit extract, 2-8 parts of a cassia seed extract, 0.5-2 parts of vitamins and 0.1-0.5 part of soy isoflavone. The preparation method comprises the following steps: firstly, respectively preparing the sea cucumber peptide, the schisandra chinensis extract and the semen cassiae extract, and then mixing, drying and forming all the raw materials. The sea cucumber polypeptide is prepared from the sea cucumber byproducts, so that high-value utilization of resources is realized; all the raw material components are scientifically matched, the obvious liver protection effect is achieved through multi-target and multi-way synergism, and the product is high in safety and suitable for daily health care and auxiliary conditioning of people suffering from liver diseases. The process is simple and feasible, and is suitable for industrial production.

The invention discloses an InDel molecular marker related to the isoflavone content of soybean grains and application of the InDel molecular marker. The marker is located at 57221341-57221412 sites of a No.18 chromosome of a soybean genome version GlycinemaxWm82. A2. V1 and comprises 72 bp insertion / deletion mutation, a high-isoflavone-content material carries an A-type allele containing a 72 bp insertion fragment, and the A-type allele is located at the site of the No.18 chromosome of the soybean genome version GlycinemaxWm82. A2. V1. The low-isoflavone-content material carries a B-type allele without the fragment. According to the present invention, the 72 bp InDel molecular marker significantly related to the isoflavone content is identified at the 57221341-57221412 site of the No.18 chromosome of the soybean genome version GlycinemaxWm82. A2. V1 for the first time, and according to the InDel molecular marker, the InDel primer sequence is designed, the PCR amplification is performed, the detection result is observed, and the isoflavone content phenotype is identified; the primer disclosed by the invention can be used for identifying and screening phenotypes of the isoflavone content of the soybean, and can be used for rapidly, accurately and effectively breeding soybean varieties with high isoflavone content, so that the breeding process of crops is accelerated, and the breeding efficiency and success rate are improved.

The present invention provides the use of soy isoflavones in the treatment of keratoconus. The present invention finds that soy isoflavones are non-toxic and do not inhibit cell growth in corneal stromal cells. Furthermore, soy isoflavones are easy to prepare and store, have wide clinical applications, and exhibit minimal side effects, making them suitable for formulation into eye drops. Experimental results demonstrate that soy isoflavones effectively inhibit the release of matrix metalloproteinase 1 and matrix metalloproteinase 3 from corneal stromal cells. Furthermore, soy isoflavones effectively enhance the elastic modulus of corneal stromal tissue.

The invention discloses a nutritional supplement for pregnant pets and a preparation method of the nutritional supplement. According to the preparation method, after chickpeas are hydrolyzed by protease, GAD enzyme and calcium chloride are added, glutamic acid is induced to be converted into GABA through calcium ions, and meanwhile free amino acid reacts with the calcium ions to generate amino acid chelated calcium; the egg yolk powder and the lactoferrin are utilized to construct a double-emulsion system, so that the GABA is protected from being damaged in advance, the amino acid chelated calcium is slowly released, and the absorption antagonism of mineral substances such as calcium, iron and zinc is reduced. The chicken breast, the chicken liver, the traditional Chinese medicines and other raw materials are chopped, mixed and sterilized to obtain a finished product. The nutritional powder is rich in GABA, soybean isoflavone, Omega-3 fatty acid, vitamin B6, lactoferrin, folic acid and various minerals, can directly or indirectly support progestational hormone secretion and relieve anxiety during pregnancy, meets the requirements of maternal blood replenishing and immunity improving and fetal development nutrition supply, and is suitable for nutrition supplement of pets during pregnancy.